Medicine and Health Sciences Commons^™

Pengaruh Deep Neck Flexor Exercise Terhadap Neck Pain Non-Specific Pada Penjahit, Salwa Maylani, Fendy Nugroho, Yoni Rustiana

Jurnal Fisioterapi Terapan Indonesia

Background: tailors tend to bow for a long time which results in excessive activation of the postural muscles in maintaining the position of the neck which causes weakness, decreased activity, and muscle imbalance which results in pain in the neck area. In overcoming these disorders, the intervention that can be given is deep neck flexor exercise. Objective: to determine the effect of deep neck flexor exercise on non-specific neck pain in tailors. Methods: this study used an experimental one group pre and post test with control design which was carried out in May-July 2024 at PT. Iskandar Indah Printing Textile …

Kecemasan Orang Tua Yang Memiliki Anak Disabilitas Intelektual Di Slbn 4 Jakarta, Hermito Gidion, Purnama Betty Sitorus, Zahrah Nabila Humayrah

Jurnal Fisioterapi Terapan Indonesia

Anxiety is a common mental condition characterized by feelings of nervousness, fear, and restlessness that can interfere with daily life. Parents who have children with intellectual disabilities often experience anxiety due to various factors, including psychological pressure and high emotional burdens. The aim of this study was to analyze the level of anxiety among parents who have children with intellectual disabilities at SLBN 4 Jakarta and to identify the relationship between predisposing and precipitating factors and the level of anxiety. This research employed a quantitative descriptive approach with a cross-sectional design. The sample consisted of 81 parents at SLBN 4 …

Hubungan Alignment Knee Joint Terhadap Kecepatan Berlari Pada Anak Usia 4-6 Tahun, Rifka Alifia Haura, Miranti Yolanda Anggita, Abdul Chalik Meidian

Jurnal Fisioterapi Terapan Indonesia

Alignment Knee joint alignment is a critical component in the biomechanical analysis of the body, particularly in locomotor activities such as running. Imbalance or malalignment in the knee joint can disrupt movement patterns and increase the risk of musculoskeletal injuries. In pediatric physiotherapy, early identification of lower limb posture is essential to support optimal gross motor development. This study aims to examine the relationship between knee joint alignment and running speed in children aged 4–6 years. A crosssectional design was used, involving 45 children who met the inclusion criteria. Measurement of knee alignment was performed using a goniometer to assess …

Pengaruh Biofeedback Terhadap Program Peningkatan Kekuatan Otot, Aditya Johan Romadhon, Fendy Nugroho, Pajar Haryatno

Jurnal Fisioterapi Terapan Indonesia

Background: In sport the muscle fiber composition surely affect athlete performances, selective training technic and using advance technology may improve and set a new standards for athlete optimal performances, technology innovation such as computer simulator has been use to generate realistic match simulation in several sport, but the technology is not cheap that many sport clubs can afford, so biofeedback may become innovation to improve athletes performance by monitoring muscle activation to achieve selective muscle contraction in muscle strengthening programs. Objective: The objective of this study was to evaluate whether auditory feedback as biofeedback can improve muscle activation in a …

Analysis Of The Acute Effects Of Self-Myofascial Release And Dynamic Stretching On Hamstring Flexibility In Individuals With Hamstring Tightness, Raisyah Intantri, Yulianto Wahyono, Sugiono -. -

Jurnal Fisioterapi Terapan Indonesia

Background: Sedentary behavior among college students leads to decreased physical activity, which negatively affects physical fitness, especially by reducing muscle flexibility. Self-Myofascial Release (SMFR) and Dynamic stretching (DS) are exercise interventions that have the potential to improve muscle flexibility. Objective : This study aimed to compare the acute effects of Self-Myofascial Release and Dynamic stretching on hamstring flexibility in students with hamstring tightness. Methods: This study employed a two-group pre-test and post-test design conducted in May at Poltekkes Surakarta. Participants were selected using simple random sampling and divided into two groups: SMFR (n=19) and DS (n=19). Each group received a …

Quality Improvement Project: Nursing Education In Relation To The Use And Effect Of Neuromuscular Blockade Agents, Lauren Danielle Bowen

Doctor of Nursing Practice Projects

Neuromuscular blockade agents are a medication class that requires education and knowledge to ensure protocols, assessments, and tools are utilized in acute care facilities. Quality improvement (QI) project focused on the use of neuromuscular blockage agents to ensure nursing staff, who have direct patient contact, received proper education. A quasi-experimental, pre-post project intervention design was used to evaluate evidence-based practice interventions with a literature review, assessment, and tools used by the population studied. Aim and purpose of the QI project was to increase nursing staff educational knowledge on the use of neuromuscular blockade agents in patients who are sedated requiring …

Restorative Masculine Integration Theory, Todd Angelucci, Julian L. Gallegos

School of Nursing Faculty Publications

The Restorative Masculine Integration Theory (RMIT) introduces a strengths-based, systems-oriented framework for promoting healthy masculinity through healing, leadership, and relational engagement. Developed in response to the adverse effects of masculine suppression, such as emotional disengagement, burnout, and polarity collapse, RMIT outlines a five-phase Cycle of Masculine Restoration: reclaimed masculinity, emotional safety and trust, grounded leadership, rebalanced gender polarity, and healing with cultural renewal. These stages are underpinned by core concepts including psychological safety, peer mentorship, emotional literacy, and authentic masculinity. Drawing from interdisciplinary foundations in gender studies, emotional intelligence, trauma-informed practice, polarity theory, and servant leadership, RMIT offers an adaptable …

Biological Impact Of Chemical-Based Medications On Human Dna: Mechanisms, Clinical Consequences, And Research Gaps, Israa M. Ali Alameen, Sumayah Mahmood Rafid, Baqer Khaled Hothefa

Al-Esraa University College Journal for Medical Sciences

Background: Not long ago DNA has become the subject of interest for the pharmaceutical companies, that because its proven by medical and scientifical fields, that it’s the fundamental sources of proteins, which is the basic element in the cell, therefore, the interaction between the Drug and the deoxyribonucleic acid (DNA) is very important to therapeutic regimens success but in other side it has a dangerous effect like toxicity, and long-term genomic instability. Objective: The aim of this review is to focus on the different studies which examines the effect of different drug like the chemotherapy, antivirals, antibiotics, and immune-suppressants on …

Application Of The Surgical Apgar Score To Predict Intensive Care Unit Admission And Post-Operative Outcomes In Cesarean Hysterectomy For Placenta Accreta Spectrum, Emily Root, Jacqueline Curbelo, Patrick Ramsey, Jessian L Munoz

Faculty, Staff and Students Publications

Background and Objective: Placenta Accreta Spectrum (PAS) encompasses a continuum of abnormal placentation conditions associated with significant maternal and fetal morbidity. Management of PAS requires coordinated cesarean hysterectomy. Associated morbidities include blood transfusion, coagulopathy, and intensive care unit (ICU) admission. Accurate prediction of ICU admission allows for enhanced multidisciplinary management, coordination of care and utilization of resources. Scoring systems exist in other surgical specialties that can predict the likelihood of ICU admission, but these have not been applied to an obstetric population. The SAS is a 10-point scale that has been validated for the prediction of ICU-level care requirements within …

Sex Differences In Cardiovascular Disease And Associated Factors In People Living With Hiv: Evidence From All Of Us Program, Hao Zhang, Huiyi Xia, Fanghui Shi, Qingyang Li, Sharon Weissman, Xiaoming Li, Xueying Yang

Faculty Publications

Background

People living with HIV (PLWH) face a higher risk of cardiovascular disease (CVD), with significant sex differences in outcomes. However, it is unclear whether these differences are driven by distinct risk factor profiles or by a differential impact of shared risk factors. This study aimed to identify factors associated with CVD among PLWH and to specifically assess for effect modification by sex using a large, diverse nationwide database.

Methods

We utilized data from the All of Us Research Program (AoU). The primary outcome was a composite of coronary artery disease or stroke. We used multivariable logistic regression to identify …

Psychological Distress And Quality Of Life Among Infertility Couples Undergoing Infertility Treatment In Malaysia, Farrahdilla Hamzah, Zamzaliza Abdul Mulud, Malini Mat Napes, Sarah Abdul Mubarak, Rosliza Shafie

Jurnal Keperawatan Indonesia

Infertility often leads to stress, anxiety, and depression, significantly affecting the quality of life of affected couples. This study explored the sociodemographic and psychological factors influencing the quality of life among infertile couples undergoing in vitro fertilization (IVF) treatment in Malaysia, using a cross-sectional design. A total of 126 infertile couples were purposively sampled from three public hospitals offering IVF treatment. The Depression, Anxiety, and Stress Scale (DASS-21) measured psychological distress levels, while the Fertility Quality of Life (Ferti-QoL) scale assessed fertility-related quality of life. Data collection adhered to strict ethical standards, with confidentiality ensured. Participants provided informed consent and …

Assessing Community Readiness And Benefits Of A Nursing Program: Implications For Healthcare And Education, Mary Anne Frances D. Reyes, Aaron A. Funa, Robert Jamisola, Ma. Joahna D. Perdigon, Jerica D. Latonero

Jurnal Keperawatan Indonesia

In response to recent policy shifts allowing the establishment of new nursing programs in the Philippines, this study assesses the perceived readiness of Sorsogon, a geographically underserved province, to implement a bachelor of science in nursing program. It also explores the anticipated benefits that such a program may offer to students and the local community. A cross-sectional survey of 375 stakeholders, selected through purposive sampling, was conducted using a validated instrument developed through expert consultation. Quantitative data were analyzed using descriptive statistics, while qualitative responses were examined through thematic analysis. The findings reveal widespread support for the proposed program, emphasizing …

Development Of A Telenursing-Based Self-Assessment Questionnaire For Diabetic Foot Ulcer Risk, Ratna Wirawati Rosyida, Sugiyarto Sugiyarto

Jurnal Keperawatan Indonesia

Development of a Telenursing-Based Self-Assessment Questionnaire for Diabetic Foot Ulcer Risk. Early identification of the risk of diabetic foot ulcers (DFU) is crucial in preventing ulcers. Caring for diabetic patients, including early detection of the risk of DFU with telenursing, will improve accessibility to health facilities and also help the work of nurses. Currently, there are many instruments or tools to detect the risk of DFU, but instruments that apply telenursing and can be used independently and easily by patients are still limited. This study aimed to develop a digital self-assessment questionnaire for DFU risk in type 2 Diabetes Mellitus …

Effectiveness Of Intradialytic Resistance Training On Muscle Mass And Strength In Patients On Hemodialysis, Sandra Vasquien, I Ketut Swarjana, A.A.A. Yuliati Darmini, I Nyoman Arya Maha Putra

Jurnal Keperawatan Indonesia

Hemodialysis (HD) patients with chronic kidney disease (CKD) lose muscle mass and strength due to protein energy wasting (PEW). The purpose of this study was to test the effectiveness of intradialytic resistance training (IRT) in increasing muscle mass and strength. It used a pre-experimental, one-group pretest-posttest design. Based on a sample size estimate using G*power software, 33 CKD patients undergoing HD at a private hospital in Bali were included. The patients were selected using purposive sampling. Muscle mass was measured using Bioelectrical Impedance Analysis (BIA), while muscle strength was measured using the Medical Research Council Muscle Scale. The Wilcoxon test …

Promoting Competence And Confidence: Simulation-Based Basic Life Support Training For Jordanian Nurses, Yousef Shukry Abu-Wardeh, Wan Muhamad Amir W Ahmad, Mohd Shaharudin Shah Che Hamzah, Intan Idiana Hassan

Jurnal Keperawatan Indonesia

Cardiopulmonary arrest is a major health issue that affects healthcare providers. The COVID-19 pandemic has added a new risk to rescuers who may be attempting to resuscitate victims. It is essential to strike a balance between resuscitation and the need to protect oneself from infection. The study aims to evaluate the effectiveness of simulation training in enhancing the Basic Life Support (BLS) knowledge, skills, and confidence levels of newly employed nurses. The study employed a single-masked, prospective, randomized controlled trial design, which consisted of three phases: a pre-test, an immediate post-test, and a post-test conducted three months later. The American …

Stage- And Smoking-Associated Microrna Expression In Lung Adenocarcinoma, Min Huang, Yimin Ge, Huiqin Chen, Caimiao Wei, David Cogdell, Cristina Ivan, Meng Chen, Wei Zhang, George A Calin, Ming Guo

Faculty, Staff and Student Publications

Background: Altered expression of microRNAs (miRNAs) is implicated in lung carcinogenesis, but little research has investigated the association of miRNA alterations with lung cancer stage or smoking status. To identify such alterations in lung adenocarcinoma, we conducted miRNA profiling.

Methods: Lung adenocarcinoma specimens and paired nonneoplastic lung tissues from 58 patients who had received no preoperative therapy and underwent tumor resection from 1991 to 2006 were collected from tumor tissue banks. Thirty (52%) of the tumors were stage I and 28 (48%) stage II or higher. Twenty-five (43%) patients were nonsmokers and 33 (57%) were smokers. To identify miRNAs of …

Real-World First-Line Outcomes Of Alectinib And Brigatinib In Anaplastic Lymphoma Kinase-Positive Non-Small Cell Lung Cancer: A Nationwide South Korean Cohort Study Using The Health Insurance Review And Assessment Data, Hee Jun Kim, Eunyoung Angela Lee, Jin-Hee Park, Hyun Woo Lee

Faculty, Staff and Student Publications

Background: Lung cancer remains the leading cause of cancer-related mortality worldwide. Anaplastic lymphoma kinase (ALK)-positive non-small cell lung cancer (NSCLC) presents unique clinical challenges, including frequent central nervous system (CNS) metastases. At present, comparative real-world data on ALK inhibitors remain limited. This study aimed to compare real-world progression-free survival (PFS) and overall survival (OS) among patients with ALK-positive NSCLC treated with first-line alectinib or brigatinib using nationwide South Korean data.

Methods: This retrospective cohort study analyzed anonymized data from South Korea's Health Insurance Review and Assessment Service, covering January 2007 to December 2023. Patients diagnosed with ALK-positive NSCLC (ICD-10: C34x) …

Assessing Hesitancy Towards Paediatric Vaccines Among Healthcare Professionals: A Descriptive Cross-Sectional Study From The United Arab Emirates, Drishti D. Kampani, Kamel A. Samara, Mohammed Mahfouz, Ahmad Hisham Al-Anoud, Amna Basel Al-Beer, Humaid Altaheri, Hiba Jawdat Barqawi, Eman Abu-Gharbieh

Abington Jefferson Health Papers

BACKGROUND: Vaccination is one of the most effective public health interventions, preventing millions of deaths globally. However, vaccine hesitancy persists, not only among the public, but also among healthcare professionals (HCPs), who serve as key sources of vaccine information. This study assesses HCPs' vaccination attitudes and estimates the prevalence and determinants of vaccine hesitancy in the UAE.

DESIGN AND METHODS: This cross-sectional, descriptive study surveyed HCPs across the UAE from March to October 2024 using a 57-item questionnaire adapted from previous literature. Univariate, bivariate (chi-squared test), and multivariate (logistic regression) analyses were conducted.

RESULTS: HCPs demonstrated generally positive attitudes towards …

Peptide Vaccines For Pediatric High-Grade Glioma And Diffuse Midline Glioma: Current Progress And Future Perspectives, Aron K Mebrahtu, Vatsal Jain, Eliese M Moelker, Alexandra M Hoyt-Miggelbrink, Katayoun Ayasoufi, Eric M Thompson

2020-Current year OA Pubs

High-grade gliomas (HGGs) and diffuse midline gliomas (DMGs) in pediatric patients carry a poor prognosis, necessitating the rapid development of novel therapies. Peptide vaccines represent a safe, repeatable, and rational immunotherapeutic modality aimed at inducing potent, tumor-specific T-cell responses. In this review, we define the scope of current progress by arguing that immunogenicity in children with HGG/DMG hinges on three factors: appropriate antigen class (neoantigen vs. TAA), the use of potent immunoadjuvants, and successful navigation of immune suppression. To address the gap between biological promise and clinical reality, we analyze clinical trials targeting shared tumor-associated antigens (e.g., CMV pp65, Survivin) …

Early And Mid-Term Outcomes Of Mitral Annular Calcification And Transcatheter Mitral Therapy: A Systematic Review, Yosuke Sakurai, Ashton Mcdonald, Tsuyoshi Kaneko

2020-Current year OA Pubs

BACKGROUND: Mitral annular calcification (MAC) poses technical and clinical challenges in patients undergoing mitral valve intervention. Patients with severe MAC were excluded from major previous randomized controlled trials evaluating mitral therapies. Consequently, outcomes following mitral transcatheter edge-to-edge repair (M-TEER) in this population remain controversial.

METHODS: MEDLINE and EMBASE were searched through June 2025 to identify studies reporting outcomes after M-TEER in patients with MAC. This systematic review assessed the difference in MAC grading and M-TEER eligibility across studies. The definition of procedural success was documented to highlight inconsistencies. The primary endpoint was all-cause mortality during follow-up. Secondary endpoints included procedural …

Potential Mechanisms Of Influence Between Spiritual Practices And Cognitive Health: A Systematic Review And Conceptual Model, Katherine Carroll Britt, Hayoung Oh, Augustine Cassis Obeng Boateng, Sherry Morgan, Sato Ashida, Corey Nagel, Roland J Thorpe, Harold G Koenig

Staff and Researcher Publications

Background/Objectives: This systematic review summarizes the evidence regarding potential mechanisms underlying the relationship between spiritual practices and cognitive health in adults. The review was performed based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist.

Methods: An extensive search of six electronic databases (i.e., PubMed, PsycINFO, Embase, Sociological Abstracts, ATLA, CINAHL) was conducted using keywords related to spiritual practices, religious activities, and cognitive health from 1 January 2000 to December 2025. The quality of the included studies was assessed using the Mixed Methods Appraisal tool aligned with the study design.

Results: A total of …

Young Adults And Early-Onset Psychosis: A Grounded Theory Study Of Experiences Of Identity Development, Katherine Hayden-Lewis, Deborah Rubel

The Qualitative Report

While identity development is a crucial task for young individuals ages 15-25, current research inadequately centralizes identity development in the context of first-time psychosis related to schizophrenia spectrum disorders. This study addresses the knowledge gap by exploring the grand research question, “How does a young person experience their identity development after the first episode of psychosis?”. Constructivist grounded theory methods influenced by critical frameworks were used to collect and analyze data with seven young adults engaged in first episode psychosis programs. Participants were young adults, between the ages of 19-24, that at the time of the study were enrolled in …

Accuracy Of Student Career Choice: Analysis Of Interest And Personality Match In The Industry 4.0 Era, Nikon Aritonang, I Nyoman Sudana Degeng, M Ramli, Adi Atmoko

Buletin Konseling Inovatif

This study aims to clarify the high number of college students choosing unsuitable career choices. The impact of choosing an unsuitable career path can significantly hinder the development of Indonesian human resources in the context of the industry 4.0 era. This is caused by shifting paradigms in the recruitment process of most companies or institutions that no longer prioritize a diploma as the key factor in seeking new hires. Instead, there is greater emphasis on the employee's skills, interests, creativity, and innovation, followed by a diploma and achievement index. Descriptive-qualitative design was employed. Samples were collected from college students at …

Quality Improvement Project: Standardized Medication Reconciliation Process Training: A Path To Improve Staff Medication Confidence, Christologus Obi

Doctor of Nursing Practice Projects

Medication reconciliation represents a critical safety process in psychiatric healthcare, where medication discrepancies affect up to 67% of hospitalized patients, with 30-40% potentially causing significant harm. This quality improvement project implemented a standardized medication reconciliation training program at a Texas mental health facility to address knowledge and confidence gaps among clinical staff. Using Kotter's 8-Step Change Model and the Iowa Model of Evidence-Based Practice, this quasi-experimental pre-post intervention study delivered three 30-minute interactive sessions to 12 multidisciplinary healthcare professionals. The evidence-based intervention introduced a 3-phase reconciliation model focused on obtaining accurate medication histories, documentation, and discrepancy resolution. Data collection used …

A Rare Titin Gene Mutation Causing Hypertrophic Cardiomyopathy In An Omani Individual, Kumayl Al-Lawati, Madan M. Maddali

Sultan Qaboos University Medical Journal

Hypertrophic cardiomyopathy is a genetic disorder in which the interventricular septum is typically more severely hypertrophied compared to other segments of the myocardium. We report a 18-year-old woman who presented in 2024 to a tertiary cardiac care centre in Muscat, Oman, with a history of anterolateral accessory pathway ablation who developed burning chest pain after stress. Transthoracic echocardiography revealed generalised left ventricular hypertrophy with maximal thickness in the infero-lateral wall with mid-cavity obstruction. The symptoms necessitated bisoprolol and disopyramide treatment. As there was an increased risk of sudden cardiac death due to left ventricular wall thickness of more than 30 …

Ensuring Safety And Optimization In Total Joint Arthroplasty Of Cancer Patients: A Comprehensive Analysis Of Complication Risks And Modifiable Risk Factors, Tara Korbal, Robert Branstetter Iv, Matthew Cable, Deryk Jones, Lizheng Shi, Lauren Hall, Andrew Chapple

School of Medicine Faculty Publications

AIMS & OBJECTIVES: The primary objective of this study is to determine whether an active cancer diagnosis results in an increased risk of perioperative TJA complications and postoperative mortality. The secondary objective is to analyze the effects of demographic factors on perioperative complication rates in cancer patients undergoing TJA. MATERIALS & METHODS: Patients with active cancer diagnoses undergoing total joint arthroplasty from 2014 to 2020 were included in this retrospective analysis. Patient data was obtained through ReachNet, which consisted of data from the University Medical Center, Tulane University Medical Center, Ochsner Health System, and Baylor Scott & White Health. ICD-9/10 …

Early Determination Of The Dorsal-Ventral Axis In Endochondral Ossification In Mice, Sixun Wu, Hirotaka Matsumoto, Jumpei Morita, Mina Yamabe, Azumi Noguchi, Shinsuke Ohba, Noriaki Ono, Yuki Matsushita

Faculty, Staff and Student Publications

Endochondral ossification is a highly coordinated process involving distinct progenitor cell populations within the mesenchymal condensation and subsequent cartilage anlage and perichondrium, all of which drive skeletal formation. Cell-type specific lineage tracing conducted to understand fetal bone development has revealed various fates of early skeletal cells. However, the underlying continuous and precise cellular dynamics of fetal skeletal cells, particularly along the dorsoventral axis, remain unclear. Here, we show that spatiotemporally specific skeletal progenitor cells in the early developmental stage contribute to the dorsal-ventral axis in a manner that is strictly determined during initial developmental stages. Lineage-tracing experiments using Fgfr3-creER and …

The Gut Microbiome In Stevens-Johnson Syndrome And Sjögren's Disease: Correlations With Dry Eye, Luciana Frizon, Talita Trevizani Rocchetti, André Frizon, Rafael Jorge Alves De Alcântara, Cintia S De Paiva, José Álvaro Pereira Gomes

Faculty, Staff and Students Publications

Changes in gut microbial composition may influence mucosal immune responses and contribute to systemic autoimmune manifestations. In this pilot exploratory study, we investigated and compared the gut microbiome in patients with Stevens–Johnson syndrome (SJS), patients with Sjögren’s disease (SjD), and healthy controls, using next-generation sequencing (NGS), and assessed correlations with dry eye parameters. The study included 10 patients with SJS matched by age and sex to 10 healthy controls, and 10 patients with SjD matched to an additional set of 10 healthy controls. Dry eye parameters were employed to evaluate dry eye disease (DED). Microbiome profiles were determined using next-generation …

Implementing And Evaluating Visual Key Information Pages Compared To Text-Based Key Information: Protocol For A Stepped-Wedge Cluster Randomized Trial, Krista E Cooksey, Eliana C Goldstein, Clara N Lee, Jessica Mozersky, Kimberly A Kaphingst, Jingxia Liu, Shuang Wu, Molly Volkmar, Victor Catalan-Gallegos, Mary C Politi

2020-Current year OA Pubs

BACKGROUND: Informed consent is a key aspect of conducting research studies, yet many participants do not understand critical study details after reviewing long, complex consent documents. The 2018 Common Rule aimed to address this challenge by requiring a key information section, a "concise and focused" presentation of important and relevant study information. In our ongoing work, we developed a template and toolkit to improve the presentation of key information by incorporating health literacy best practices, including color and visual icons. The goal of this study is to assess the impact of the visual key information (KI) pages at both the …

Protrack3d: A Comprehensive Tool For Segmentation And Tracking Of Proteins With Split And Fusion, Ramu Gautam, Yang Jiao, Yasong Pang, Mo Weng, Mei Yang

Electrical & Computer Engineering Faculty Research

Background: Quantitatively tracking subcellular protein puncta over time is critical for understanding protein functions and related cellular processes, as many proteins assemble into discrete protein puncta when performing functions. Monitoring these protein puncta over an extended period enables the quantitation of their dynamic behaviors, such as repositioning, remodeling, and inter-object interactions such as fusion and split. These characteristics are essential for deciphering the underlying regulatory mechanisms of protein function. However, tracking protein puncta is challenging due to these clusters undergoing rapid and complex temporal changes.

Results: To address these challenges, a comprehensive tool ‘ProTrack3D’ is developed. It implements a multi-stage …