Medicine and Health Sciences Commons^™

Advantages Of Whole Genome Sequencing In Mitigating The Helicobacter Pylori Antimicrobial Resistance Problem, Kartika Afrida Fauzia, Ricky Indra Alfaray, Yoshio Yamaoka

Faculty, Staff and Students Publications

Helicobacter pylori antimicrobial resistance is a critical public health issue. Typically, antimicrobial resistance epidemiology reports include only the antimicrobial susceptibility test results for H. pylori. However, this phenotypic approach is less capable of answering queries related to resistance mechanisms and specific mutations found in particular global regions. Whole genome sequencing can help address these two questions while still offering quality control and is routinely validated against AST standards. A comprehensive understanding of the mechanisms of resistance should improve H. pylori eradication efforts and prevent gastric cancer.

Perspectives On Non-Prescription Antibiotic Use Among Hispanic Patients In The Houston Metroplex, Lindsey Laytner, Patricia Chen, Susan Nash, Michael K Paasche-Orlow, Richard Street, Roger Zoorob, Barbara Trautner, Larissa Grigoryan

Faculty, Staff and Students Publications

BACKGROUND: Non-prescription antibiotic use includes taking an antibiotic without medical guidance (e.g., leftovers, from friends/relatives, or purchased without a prescription). Non-prescription use contributes to antimicrobial resistance, adverse drug reactions, interactions, superinfection, and microbiome imbalance. Qualitative studies exploring perspectives regarding non-prescription use among Hispanic patients are lacking. We used the Kilbourne Framework for Advancing Health Disparities Research to identify factors influencing patients' non-prescription use and organize our findings.

METHODS: Our study includes Hispanic primary care clinic patients with different types of health insurance coverage in the Houston metroplex who endorsed non-prescription use in a previous survey. Semistructured interviews explored the factors …

Dna Supercoiling-Induced Shapes Alter Minicircle Hydrodynamic Properties, Radost Waszkiewicz, Maduni Ranasinghe, Jonathan M Fogg, Daniel J Catanese, Maria L Ekiel-Jeżewska, Maciej Lisicki, Borries Demeler, Lynn Zechiedrich, Piotr Szymczak

Faculty, Staff and Students Publications

DNA in cells is organized in negatively supercoiled loops. The resulting torsional and bending strain allows DNA to adopt a surprisingly wide variety of 3-D shapes. This interplay between negative supercoiling, looping, and shape influences how DNA is stored, replicated, transcribed, repaired, and likely every other aspect of DNA activity. To understand the consequences of negative supercoiling and curvature on the hydrodynamic properties of DNA, we submitted 336 bp and 672 bp DNA minicircles to analytical ultracentrifugation (AUC). We found that the diffusion coefficient, sedimentation coefficient, and the DNA hydrodynamic radius strongly depended on circularity, loop length, and degree of …

Country-Level Gender Inequality Is Associated With Structural Differences In The Brains Of Women And Men, André Zugman, Luz María Alliende, Vicente Medel, Richard A.I. Bethlehem, Jakob Seidlitz, Grace Ringlein, Celso Arango, Aurina Arnatkevičiūtė, Laila Asmal, Anderson M. Winkler

School of Medicine Publications

Significance

Gender inequality is associated with worse mental health and academic achievement in women. Using a dataset of 7,876 MRI scans from healthy adults living in 29 different countries, we here show that gender inequality is associated with differences between the brains of men and women: cortical thickness of the right hemisphere, especially in limbic regions such as the right caudal anterior cingulate and right medial orbitofrontal, as well as the left lateral occipital, present thinner cortices in women compared to men only in gender-unequal countries. These results suggest a potential neural mechanism underlying the worse outcome of women in …

Rbm47 Regulates Intestinal Injury And Tumorigenesis By Modifying Proliferation, Oxidative Response, And Inflammatory Pathways, Saeed Soleymanjahi, Valerie Blanc, Elizabeth A. Molitor, David M. Alvarado, Yan Xie, Vered Gazit, Jeffrey W. Brown, Kathleen Byrnes, Ta-Chiang Liu, Jason C. Mills, Matthew A. Ciorba, Deborah C. Rubin, Nicholas O. Davidson

2020-Current year OA Pubs

RNA-binding protein 47 (RBM47) is required for embryonic endoderm development, but a role in adult intestine is unknown. We studied intestine-specific Rbm47-knockout mice (Rbm47-IKO) following intestinal injury and made crosses into ApcMin/+ mice to examine alterations in intestinal proliferation, response to injury, and tumorigenesis. We also interrogated human colorectal polyps and colon carcinoma tissue. Rbm47-IKO mice exhibited increased proliferation and abnormal villus morphology and cellularity, with corresponding changes in Rbm47-IKO organoids. Rbm47-IKO mice adapted to radiation injury and were protected against chemical-induced colitis, with Rbm47-IKO intestine showing upregulation of antioxidant and Wnt signaling pathways as well as stem cell and …

Polarized Localization Of Phosphatidylserine In The Endothelium Regulates Kir2.1, Claire A. Ruddiman, Richard Peckham, Melissa A. Luse, Yen-Lin Chen, Maniselvan Kuppusamy, Bruce A. Corliss, P. Jordan Hall, Chien-Jung Lin, Shayn M. Peirce, Swapnil K. Sonkusare, Robert P. Mecham, Jessica E. Wagenseil, Brant E. Isakson

2020-Current year OA Pubs

Lipid regulation of ion channels is largely explored using in silico modeling with minimal experimentation in intact tissue; thus, the functional consequences of these predicted lipid-channel interactions within native cellular environments remain elusive. The goal of this study is to investigate how lipid regulation of endothelial Kir2.1 - an inwardly rectifying potassium channel that regulates membrane hyperpolarization - contributes to vasodilation in resistance arteries. First, we show that phosphatidylserine (PS) localizes to a specific subpopulation of myoendothelial junctions (MEJs), crucial signaling microdomains that regulate vasodilation in resistance arteries, and in silico data have implied that PS may compete with phosphatidylinositol …

Phase Ib And Expansion Study Of Gemcitabine, Nab-Paclitaxel, And Ficlatuzumab In Patients With Metastatic Pancreatic Cancer, Kimberly Perez, Koushik Das, Et Al.

2020-Current year OA Pubs

BACKGROUND: In preclinical pancreatic ductal adenocarcinoma (PDAC) models, inhibition of hepatocyte growth factor (HGF) signaling using ficlatuzumab, a recombinant humanized anti-HGF antibody, and gemcitabine reduced tumor burden.

METHODS: Patients with previously untreated metastatic PDAC enrolled in a phase Ib dose escalation study with 3 + 3 design of 2 dose cohorts of ficlatuzumab 10 and 20 mg/kg administered intravenously every other week with gemcitabine 1000 mg/m2 and albumin-bound paclitaxel 125 mg/m2 given 3 weeks on and 1 week off. This was followed by an expansion phase at the maximally tolerated dose of the combination.

RESULTS: Twenty-six patients (sex, 12 male:14 …

Introducing Motivational Interviewing Skills To Community Health Workers, Stella Antony

DNP Qualifying Manuscripts

Abstract

Background: Rural population accounts for 15% of the population in the United States of America. Rural residents have a higher risk of health disparities than urban populations. The significant health inequities in the underprivileged population include a higher incidence of chronic diseases, lack of access to healthy foods, unhealthy eating habits, lack of access to health care, poor socioeconomic status, lack of health insurance, cultural and language barriers, and lack of health literacy. Local Problem: The population in Fresno and Tulare Counties in Central Valley, California is underprivileged, lacks access to proper health care and has higher rates of …

Promoting Mindfulness Based Self-Care In Healthcare Staff, Harkirat K. Bajwa

DNP Qualifying Manuscripts

Background: Healthcare personnel (HCP) like physicians, nurses, advanced practice nurses etc. are at high risk of stress, burnout, and compassion fatigue (CF). Nearly half of the physicians and one-third of the nurses experience burnout in the United States. The physical and mental health of HCPs is impacted by burnout and CF, leading to increased practice errors and decreased patient safety.

Local Problem: HCPs have poor knowledge about mindfulness and its benefits. Mindfulness-based self-care measures are not promoted in HCP.

Methods: An extensive database search was done to find evidence about using mindfulness-based strategies to relieve burnout, stress, and compassion fatigue …

5-Prong Approach Taken By Tsu College Of Pharmacy And Health Sciences To Mitigate Covid-19, Zuri Dale, Rashid Mosavin, Cynthia Johnson

COVID Research

The TSU COVID-19 Implementation and Response White Paper outlines Texas Southern University's (TSU) multifaceted approach to managing the COVID-19 pandemic, led by the College of Pharmacy and Health Sciences (COPHS). The initiative, focused on the safety of the TSU community, included creating a COVID Prevention Center (CPC) to enhance collaboration, public health adherence, and campus-wide surveillance.

Key Strategies:

1. Education and Communication: Weekly informational broadcasts, social media engagement, and an internal COVID guide aimed to inform the TSU community and counter vaccine hesitancy.
2. Integration and Coordination: Campus services were streamlined to improve communication and enhance response efficiency, with a comprehensive guide …

Breaking The Stigma: Challenging The Mental Health Crisis Through A New Way Of Designing A Recovery Facility In Salt Lake City, Utah, Lindsey Denig

Bachelor of Architecture Theses - 5th Year

Mental health is a highly stigmatized topic within today’s society. The mental health system in the United States has failed to consistently create safe and successful spaces for recovering patients with psychological disorders.

Facilities are often described as “inhumane” by patients in how they are designed. Patients have expressed that there is lack of safe, private spaces. The spaces that are considered “private” are solitary confinement wards with no windows or furniture. It is said that interior design is not a priority as well, with no colors or art on walls. Spaces are cold and dead feeling, which does not …

Structure-Guided Mutagenesis Reveals The Catalytic Residue That Controls The Regiospecificity Of C6-Indole Prenyltransferases, Ahmed R. Aoun, Nagaraju Mupparapu, Diem N. Nguyen, Tae Ho Kim, Christopher M. Nguyen, Zhengfeiyue Pan, Sherif I. Elshahawi

Pharmacy Faculty Articles and Research

Indole is a significant structural moiety and functionalization of the C−H bond in indole-containing molecules expands their chemical space, and modifies their properties and/or activities. Indole prenyltransferases (IPTs) catalyze the direct regiospecific installation of prenyl moieties on indole-derived compounds. IPTs have shown relaxed substrate flexibility enabling them to be used as tools for indole functionalization. However, the mechanism by which certain IPTs target a specific carbon position is not fully understood. Herein, we use structure-guided site-directed mutagenesis, in vitro enzymatic reactions, kinetics and structural-elucidation of analogs to verify the key catalytic residues that control the regiospecificity of all characterized regiospecific …

Elucidating The Impact Of Sos-Response Timing In On Escherichia Coli Survival Following Treatment With Fluoroquinolone Topoisomerase Inhibitors, Stephanie Schofield

Honors Scholar Theses

Antibiotic treatment failure is a public health crisis, with a 2019 report stating that roughly 35,000 deaths occur in the United States yearly due to bacterial infections that are unresponsive to antibiotics (1). One complication in the treatment of bacterial infection is antibiotic persistence which further compromises our battle to effectively treat infection. Bacterial persisters can exist in clonal bacterial cultures and can tolerate antibiotic treatment by undergoing reversible phenotypic changes. They can survive drug concentrations that their genetically identical kin cannot. Some persisters remain in a slow growing state and are difficult to target with current antibiotics. A specific …

Telehealth And Its Impact On The Management Of Type 2 Diabetes Mellitus, Melissa N. Toulios

Evidence-Based Practice Project Reports

Diabetes mellitus type 2 (T2DM) is a growing epidemic that affects millions of people throughout the world. The recent COVID-19 pandemic has created many new challenges, including access to health care for treatment of chronic diseases, such as diabetes (Robson & Hosseinzadeh, 2021). Telehealth has offered a promising approach to improve the management of diabetes(Bellman, 2021). People with diabetes are less likely to manage their diabetes through the usual care because of risk of contracting COVID-19 (CDC, 2022). The purpose of this EBP project was to evaluate the effectiveness of T2DM management through a telehealth approach. This project has addressed …

Cultural Humility When Caring For Lgbtqia+ Older Adults: A Resource Guide For Occupational Therapy Practitioners And Students, Michele Ramos, Deb Meyers, Mary Ann Smith

Spring 2023 Virtual OTD Capstone Symposium

The LGBTQIA+ older adult population has unique needs due to their experience as diverse individuals in a cisgender, heteronormative society. Experiences and effects of discrimination need to be considered when providing care. Occupational therapists have a role in addressing disparities of all marginalized groups, including LGBTQIA+ older adults. Practitioners may utilize cultural humility and trauma-informed practices when treating the LGBTQIA+ population. Existing resources to guide culturally humble occupational therapy care for LGBTQIA+ older adults are insufficient. The purpose of this project was to build on existing cultural humble resources and create a website on the focus of occupational therapy cultural …

An Exploratory Qualitative Study Of Oncology Nurses’ Experience In Providing Care For Terminally Ill Patients In Salmaniya Medical Complex In Bahrain, Layla S. Turki, Leena Khonji, Magda Bayoumi

The Qualitative Report

Oncology nurses are essential in palliative care for cancer patients, especially at end-of-life stages. Caring for terminally ill cancer patients is stressful, accompanied by negative and positive feelings, but oncology nurses have an unavoidable responsibility. However, little is known about oncology nurses' experience in the Kingdom of Bahrain caring for terminally ill cancer patients in their final weeks or days. This study aimed to explore the experience of oncology staff nurses in providing care for terminally ill patients in the Kingdom of Bahrain, and the research answered those questions: (a) What is the experience of oncology staff nurses in providing …

Supporting Adoption Of Atraumatic Care By Rural Hospital Nursing Staff Through Education, Angie Sowers

Doctoral Projects

Problem Statement: While atraumatic care is often practiced in children's specialty hospitals, it is less likely to be a part of the care provided in rural hospitals (Li et al., 2018). Nurses in this setting may not feel equipped or supported to provide atraumatic care.

Purpose: The purpose of this DNP Project is to support increased knowledge and utilization of atraumatic care by nursing staff providing care for pediatric patients in rural community hospital through a professional development education program. Methods: An educational program development project was created, implemented, and evaluated to support the utilization, awareness, and knowledge of atraumatic …

Evaluation Of The Effectiveness Of The Ihi Joy In Work Framework Among Nurses, Jezlynn M. Vonnahme

Doctor of Nursing Practice Projects: College of Nursing

Background: Registered nurses are leaving the bedside at alarming numbers with many of them suffering from burnout, a decrease in compassion satisfaction, a lack of joy in work, and/or lack of work-life balance. It is critical these issues within nursing are identified and mitigated in order to reduce the number of nurses planning on leaving the bedside as 33% of nurses are currently considering leaving their role or reducing their hours. This statistic does not help with the current nursing shortage and its prediction to continue to grow with employment needs for nurses expected to increase 9% more than any …

International Strategies For Addressing The Needs Of Children With Disabilities: Comparing Pediatric Therapy Services In Italy And The United States, Cassidy Hearn, Lillian Hsiao, Lavinia Fanelli, Regina Harbourne

Physical Therapy Projects

This paper introduces and compares the professions of Neuro and Psychomotor Therapist of Developmental Age and pediatric physical therapy. Although pediatric physical therapy practice is prevalent in the United States, Italy utilizes another profession which encompasses many features of physical therapy, but also incorporates neuropsychological factors, specifically for children.

Implementation Of A Nurse-Led Clinical Algorithm For Pressure Injury Prevention Associated With Non-Invasive Ventilation Medical Device Use, Capri Steinkuhler, Kaitlin Rogge

Doctor of Nursing Practice Projects: College of Nursing

Purpose and Aims

Medical device related pressure injuries (PI) account for as much as 61-81% of all hospital reported wound incidents (Gefen et al., 2020) and are frequently caused by respiratory devices such as non-invasive ventilation (NIV). Use of NIV in critically ill patients dramatically increased during the pandemic. PI are one of the leading causes of injury to hospitalized patients and lengthens hospital stays (National Pressure Injury Advisory Panel [NPIAP], 2019). The NPIAP recommends routine skin assessment under devices, proper mask fit, protective barrier, moisture reduction, and interdisciplinary collaboration to prevent PIs from NIV masks. This feasibility study’s purpose …

Implementation Of Chronic Obstructive Pulmonary Disease Action Plan On The Step Down Critical Care Unit: A Pilot Study, Peter Chambers, Kaelee Dameron, Carolyn Kiefer

Doctor of Nursing Practice Projects: College of Nursing

Background: Hospital readmissions due to chronic obstructive pulmonary disease (COPD) exacerbations are a significant economic and resource burden on the United States healthcare system. Current professional guidelines for the prevention of acute exacerbations of chronic obstructive pulmonary disease (AECOPD) include providing patient-focused education with a written action plan such as a COPD Action Plan.

Aims: The primary purpose of this project was to determine if providing an educational intervention to patients admitted for AECOPD would reduce hospital readmission rates for COPD and improve patient outcomes. Method: We performed a quasi-experimental pilot study of 10 participants admitted with a COPD exacerbation …

Pediatric Diabetic Ketoacidosis Fluid Composition And Rate In Care Of Type 1 Diabetic Patients, Gabriel Hansen, Janae Langner, Michaela Tarpley

Doctor of Nursing Practice Projects: College of Nursing

Objective

Complications of diabetic ketoacidosis (DKA) include iatrogenic hyperchloremia and acute kidney injury (AKI). The objective of this study was to evaluate the association of decreasing sodium chloride (NaCl) composition in standard DKA fluid resuscitation and the rates/severity of hyperchloremia and AKI in pediatric patients.

Methods

In December 2020, Children’s Hospital and Medical Center (CH&MC) changed the standard base composition of DKA fluids from 0.9% to 0.675% sodium chloride (NaCl) and increased the fluid resuscitation rate for patients over 30kg as a quality improvement project. A retrospective chart review analyzed patients admitted with DKA from January 2019-December 2021, excluding patients …

Improving Residents' Access To Occupational Therapy Services In Long-Term Care Facilities: A Screen For Declining Performance In Activities Of Daily Living, Amy N. Scheidecker

Occupational Therapy Capstone Presentations

The purpose of this study was to develop a tool for long-term care (LTC) nursing staff and occupational therapy (OT) practitioners to use as a method for tracking LTC residents’ decline in activities of daily living (ADL) performance and overall function. LTC residents with intact cognition were recommended by OT practitioners and verbally recruited to participate in the background interview process. LTC nursing staff interviews were distributed at nursing stations on the LTC units for voluntary participation from nursing staff. OT/OTA interviews were manually distributed to OT practitioners. The interviews used for each population varied slightly to accommodate their role …

Welcoming Wellness: A Health Promotion And Chronic Condition Prevention Program Rooted In The Foundations Of Occupational Therapy, Karlee G. Duncan

Occupational Therapy Capstone Presentations

Background: Despite the national $3.8 billion spent annually on chronic condition management, they are still the leading cause of death and disability in the United States. Chronic conditions have a variety of global implications. The development of chronic conditions can be managed through education and lifestyle behavior modifications, which lies within the scope of occupational therapy. Method: Simultaneous experiences at Logan Health and Logan Health Medical Fitness Center informed the development and implementation of a health promotion and prevention program, Welcoming Wellness. Results: Treating clients in acute care improved my understanding of chronic condition presentation and population needs, and connecting …

Evaluating Nurse Use And Satisfaction With Video-Based Discharge Education For Adults With Heart Failure, Krystyna Simon Rn, Bsn, Molly Hendrickson Rn, Bsn, Windy Alonso Phd, Rn, Fhfsa

Doctor of Nursing Practice Projects: College of Nursing

Background

Heart failure (HF) readmission rates are high and influenced by self-care practices and self-efficacy for managing chronic disease. Using digital education via tablet can help improve the discharge education process and may increase self-care knowledge.

Objective

The purpose of the study is to assess the current discharge processes implemented for HF patients in cardiovascular units at a Midwestern academic medical center.

Methods

Participants for this two-phase study were recruited from two inpatient cardiovascular units within a 700-bed, Midwestern academic medical center. Phase one used a pre-post quasiexperimental design to evaluate a discharge educational intervention using the teach-back method in …

Identifying The Effects Of The Covid-19 Pandemic On Individuals With Dual Diagnoses (Mental Health And Substance Use) At Centerpointe Residential Treatment Center, Casey Granger, Dakota Sonderup, William Stowell-Flores

Doctor of Nursing Practice Projects: College of Nursing

Background: Social distancing measures associated with the COVID-19 pandemic began on March 28^th, 2020in the United States. With these implemented measures, the mental health of the population was worsening due to isolation, closures, and fear. Rates of anxiety, depression, and co-occurring substance abuse increased as well. CenterPointe is a behavioral health organization that has a residential treatment facility in Omaha, Nebraska for patients with mental health and substance use disorder dual diagnoses. They utilize the DLA-20 tool with their patients at intake, every 90 days, and at discharge. The DLA-20 is a standardized tool that assesses an individual’s …

Self-Managment Of Urinary Incontinence In Women, Abbey Klein

Theses & Dissertations

Urinary incontinence is experienced by approximately 60% of adult women in the United States and negatively impacts many aspects of physical and psychological health. Many women do not seek care for urinary incontinence due to social stigma. However, those who do seek care do not often receive treatment options that align with clinical guidelines. Urinary incontinence self-management has been used interchangeably in the literature with other terms, like self-care and coping, but no conceptual definition has been delineated. The overall purpose of this dissertation was to describe urinary incontinence self-management in women. To accomplish this, a concept analysis was conducted …

Mechanisms Of Mutated Myosin Vb Mediated Cholestasis In Microvillus Inclusion Disease, Melissa S. Holzapfel

Theses & Dissertations

Microvillus inclusion disease (MVID) is a constitutive intestinal epithelial disease which causes definitive intestinal failure and cholestatic liver disease primarily caused by mutations in the Myosin Vb gene. Genotype-clinical phenotype associations suggest that the progression of liver disease can be predicted through a correlation between myosin Vb residual function and variable retention of bile salt transport machinery in intestinal enterocytes and liver hepatocytes. We proposed that aberrant distribution of bile salt transport proteins disrupts enterohepatic regulation of bile acids and contributes to the progression of cholestatic liver disease. To investigate the genotype-phenotype correlation, samples from MVID patients were evaluated for …

Investigating Host Cell Transcriptional Heterogeneity And Metabolism In Staphylococcus Aureus Biofilm Infections, Christopher M. Horn

Theses & Dissertations

Staphylococcus aureus (S. aureus) is a leading cause of prosthetic joint infection (PJI). These infections are often intractable due to biofilm formation, which are complex bacterial communities that adhere to biotic and abiotic surfaces. Within a biofilm, there is considerable heterogeneity in bacterial metabolism and gene expression that contributes to the establishment an anti-inflammatory milieu at the site of infection, which favors bacterial persistence even in the context of an immunocompetent host. This is due, in part, to the preferential recruitment of granulocytic myeloid-derived suppressor cells (G-MDSCs), which are a population of pathologically activated immature leukocytes that develop …

Multilingualism And Augmentative Alternative Communication: A Review Of The Literature, Hannah N. Lamarca, Natalie R. Andzik

Honors Capstones

Purpose: Individuals from culturally and linguistically diverse backgrounds who use Alternative and Augmentative Communication (AAC) are often offered English-only AAC systems that do not meet their language needs. Devices that host multiple languages often only offer one at a time.

Method: The authors reviewed the literature by searching relevant databases using a variety of search strings. Following our inclusionary and exclusionary criteria, 15 articles were yielded for analysis.

Results: Authors found that more than half of the included articles were discussion-based papers on AAC delivery, barriers to AAC and multilingualism, and codeswitching using AAC. The included interview studies aimed to …