Medicine and Health Sciences Commons^™

Variant-Specific Pathophysiological Mechanisms Of Aff3 Differently Influence Transcriptome Profiles, Sissy Bassani, Jacqueline Chrast, Giovanna Ambrosini, Norine Voisin, Frédéric Schütz, Alfredo Brusco, Fabio Sirchia, Lydia Turban, Susanna Schubert, Rami Abou Jamra, Jan-Ulrich Schlump, Desiree Demille, Pinar Bayrak-Toydemir, Gary Rex Nelson, Kristen Nicole Wong, Laura Duncan, Mackenzie Mosera, Christian Gilissen, Lisenka E L M Vissers, Rolph Pfundt, Rogier Kersseboom, Hilde Yttervik, Geir Åsmund Myge Hansen, Marie Falkenberg Smeland, Kameryn M Butler, Michael J Lyons, Claudia M B Carvalho, Chaofan Zhang, James R Lupski, Lorraine Potocki, Leticia Flores-Gallegos, Rodrigo Morales-Toquero, Florence Petit, Binnaz Yalcin, Annabelle Tuttle, Houda Zghal Elloumi, Lane Mccormick, Mary Kukolich, Oliver Klaas, Judit Horvath, Marcello Scala, Michele Iacomino, Francesca Operto, Federico Zara, Karin Writzl, Aleš Maver, Maria K Haanpää, Pia Pohjola, Harri Arikka, Anneke J A Kievit, Camilla Calandrini, Christian Iseli, Nicolas Guex, Alexandre Reymond

Faculty, Staff and Students Publications

BACKGROUND: We previously described the KINSSHIP syndrome, an autosomal dominant disorder associated with intellectual disability (ID), mesomelic dysplasia and horseshoe kidney, caused by de novo variants in the degron of AFF3. Mouse knock-ins and overexpression in zebrafish provided evidence for a dominant-negative mode of action, wherein an increased level of AFF3 resulted in pathological effects.

METHODS: Evolutionary constraints suggest that other modes-of-inheritance could be at play. We challenged this hypothesis by screening ID cohorts for individuals with predicted-to-be damaging variants in AFF3. We used both animal and cellular models to assess the deleteriousness of the identified variants.

RESULTS: We identified …

Propofol Enhancement Of Slow Wave Sleep To Target The Nexus Of Geriatric Depression And Cognitive Dysfunction: Protocol For A Phase I Open Label Trial, Rachel Lynn Rios, Michael Green, S Kendall Smith, Mohammadmehdi Kafashan, Shinung Ching, Nuri B Farber, Nan Lin, Brendan P Lucey, Charles F Reynolds, Eric J Lenze, Ben Julian Agustin Palanca, Swiped Study Team

2020-Current year OA Pubs

INTRODUCTION: Late-life treatment-resistant depression (LL-TRD) is common and increases risk for accelerated ageing and cognitive decline. Impaired sleep is common in LL-TRD and is a risk factor for cognitive decline. Slow wave sleep (SWS) has been implicated in key processes including synaptic plasticity and memory. A deficiency in SWS may be a core component of depression pathophysiology. The anaesthetic propofol can induce electroencephalographic (EEG) slow waves that resemble SWS. Propofol may enhance SWS and oral antidepressant therapy, but relationships are unclear. We hypothesise that propofol infusions will enhance SWS and improve depression in older adults with LL-TRD. This hypothesis has …

Tcaf1 Promotes Trpv2-Mediated Ca2+ Release In Response To Cytosolic Dna To Protect Stressed Replication Forks, Lingzhen Kong, Chen Cheng, Abigael Cheruiyot, Jiayi Yuan, Yichan Yang, Sydney Hwang, Daniel Foust, Ning Tsao, Emily Wilkerson, Nima Mosammaparast, Michael B Major, David W Piston, Shan Li, Zhongsheng You

2020-Current year OA Pubs

The protection of the replication fork structure under stress conditions is essential for genome maintenance and cancer prevention. A key signaling pathway for fork protection involves TRPV2-mediated Ca

Abdominal Congestion As A Predictor Of Worsening Renal Function In Patients With Acute Decompensated Heart Failure, Hossameldin Rezk, Ghada (Sayed) Youssef, Karim Said, Iman (Atef) Mandour, Magdy Abdelhamid

Journal of the Saudi Heart Association

Objective: Worsening renal function (WRF) is a frequent finding in patients with acute decompensated heart failure (ADHF) and is a powerful independent prognostic factor for adverse outcomes. The link between abdominal congestion and worsening renal function in such patients is not yet fully addressed. The aim of this study was to evaluate the role of abdominal congestion in the early prediction of worsening renal function in hospitalized patients with acute decompensated heart failure. Methods: This was a prospective study that enrolled 100 patients with a diagnosis of ADHF and received intravenous diuretic therapy. Intra-abdominal pressure (IAP), splenic Doppler impedance indices …

Tracking Population Decline In India Between 1950-2023 And Total Deaths Cases By Cause – A Case Study, Sumayya Rehaman Research Scientist

International Journal of Health and Allied Sciences

The Case study digs the facets of the India’s population decline from 1950-2023. Focusing Total fertility rate recorded 2.0 per woman in 2023 as similar as Birth Rate since 1950-2023 and found to be 16.9 births per 1000 people in 2023. Contradictorily, may surpass China’s population in Future. It focuses Population pyramid with annual Percent growth rate divulges Main cities population and exemplifies death occurrence. The median age - 28.7 years, life expectancy - 70.4 years, Infant Mortality Rate - 26.6 deaths per 1000 live births, with 3.89% in 2023.This study reveals comparative illustrative graphs of Indian Population 1950-2023.

Effectiveness Of Oil Pulling Using Coconut Oil And Sesame Oil In Comparison With 0.2% Chlorhexidine And Placebo In Plaque Reduction- A Randomized Control Trial’, Bharath Shivakumar, Sunitha S, Chandrashekar Br, Mirunalini Sundaravadivelu

International Journal of Health and Allied Sciences

Context: Oil pulling has multiple oral health benefits toward gingival and overall health. Aims: To evaluate the effectiveness of oil pulling using sesame and coconut oils. Settings and Design: JSS High School, Suttur, Nanjangud Taluk, Mysuru Randomised controlled double-blinded parallel-arm study. Methods and Material: Participants selected were divided into four groups randomly. Mouth rinse bottles containing coconut oil, sesame oil, chlorhexidine and placebo were coded and distributed. Follow up was done on the seventh day and fourteenth day. Results: The study groups showed statistically significant difference. Conclusions: Oil pulling is effective in oral plaque control.

Examining The Impact Of Internal Family Systems (Ifs) Theory Versus Emotionally Focused Individual Therapy (Efit) On Depression For College Students: A Comparative Study, Laura Anne Bowlin, Hillary Bucher-Brown

School of Psychology, Family, and Community Research Conference

In light of the prevalence of depression in modern society, researchers persist in the quest for effective treatment approaches. This research proposal aims to examine the impact of Internal Family Systems (IFS) Theory and Emotionally Focused Individual Therapy (EFIT) on depression among college students. The study will involve 20 college students recruited from local universities experiencing depression, as measured by the Beck Depression Inventory (BDI). Each participant will undergo ten psychotherapy sessions, randomly assigned to a certified IFS or EFIT therapist. Subsequently, participants will complete a post-test using the BDI. Comparative analysis of the outcomes between the two groups will …

Effect Of Emotionally Focused Couples Therapy On Couples' Reported Satisfaction And Intimacy: A Comparison Between Participants With And Without Simultaneous Individual Therapy, Aishleen O'Brien

School of Psychology, Family, and Community Research Conference

While there is ample Emotionally Focused Therapy based research, exploration of simultaneous individual therapy’s importance is limited. This study aims to establish the significance of participating in individual therapy while also joining a partner in emotionally focused couples’ therapy. Individual therapy, in tandem with couples therapy, provides an opportunity to explore one’s own attachment style, as it is interwoven into a romantic relationship. Ultimately, by discerning the nuanced interplay between individual and couples therapy, clinicians can offer tailored, efficacious recommendations that enhance the likelihood of successful outcomes and promote enduring relationship satisfaction.

Is Emotionally Focused Family Therapy Effective In Alleviating Symptoms Of Bulimia Nervosa In Adolescents?, Susan Leonardson

School of Psychology, Family, and Community Research Conference

This study will investigate the effectiveness of Emotionally Focused Family Therapy (EFFT) in alleviating symptoms of bulimia nervosa (BN) in adolescents. Participants will consist of twenty adolescents enrolled in the Emily Program, with a DSM diagnosis of moderate to severe BN, and at least one of their caregivers. The subjects will attend twelve family therapy sessions with a therapist trained in EFFT, and complete the Eating Disorder Examination (EDE) questionnaire. This study aims to determine if EFFT can effectively improve family dynamics to reduce symptoms of BN in adolescents.

Internal Family Systems And Codependency: Finding Self In Relationship With Another Person’S Addiction, Jillian Ostlund, Mandah Gombosuren

School of Psychology, Family, and Community Research Conference

Codependency is a concept that is currently not considered a disorder in the DSM-V, yet people have been identifying with the traits of codependency since the 1940's (Bacon et al., 2018). In IFS the concept of blending means Parts are not able to differentiate from one another, making it difficult to sense Self Leadership (Schwartz & Sweezy, 2020). The researchers for this proposal were not able to find literature on the treatment of codependency using IFS. It is believed that IFS can help a codependent person to gain access to Self and be able to recover from the effects of …

Therapist Secret Policies And Client Retention, Alexandria Packard, Allison Hanigan

School of Psychology, Family, and Community Research Conference

No-secrets policies are commonly used by many couple therapists to ensure transparency between each individual and the therapist. The current literature is divided on whether the existence of a no-secrets policy creates barriers to treatment. The present study aims to assess the impact of a no-secrets policy on couple retention rates in therapy. We propose to study this by assigning a group of couples to a therapist without a no-secrets policy, and another group of couples to a therapist with a no-secrets policy. Therapy retention will be measured and analyzed as influenced by the independent variable: the no-secrets policy.

What Is The Comparative Effectiveness Of Specific Therapy Modalities In Improving Mental Health Outcomes For Black And Indigenous Clients?, Hailey Clum

School of Psychology, Family, and Community Research Conference

Black and Indigenous clients are frequently underrepresented in therapeutic research and treatment, despite the increase of individuals from these communities seeking therapy services. This qualitative study has been initiated to highlight the lack of supporting evidence for best treatment practices for Black and Indigenous-identifying clients. The goal of this study is to provide evidence-based treatment for master's and doctoral-level clinicians who work with Black and Indigenous patients. The study will analyze various treatment groups, including Black and Indigenous adult participants; and will utilize treatments and interventions from Internal Family Systems (IFS) therapy and Art Therapy.

Navigating Transnational Ties And Belonging: An Intergenerational Family Therapy Approach With South Asian Immigrant Families, Shreya Rangwani

School of Psychology, Family, and Community Research Conference

The diversity of transnational experiences of families and the challenges to their sense of belonging have important implications for the broader sociocultural mental health system. This study aims to examine intergenerational family therapy to support South Asian immigrant families as they deal with the challenges of maintaining cross-cultural relationships. Family therapy for South Asian immigrants have focused on cultural stress and intergenerational conflicts, rather than family-level methods and systemic elements that form the reports of those transnational families. Through this research, the study targets to provide vital insights and realistic techniques for MFT therapists working with South Asian immigrant populations. …

Impact Of Sibling Support On Mitigating The Effects Of Parental Divorce On Children's Attachment Security, Alana Harris

School of Psychology, Family, and Community Research Conference

The study aims to explore the potential mitigating effects of supportive sibling relationships on the attachment security of children from divorced families. Utilizing the Child Attachment Interview (CAI) and Sibling Relationship Questionnaire (SRQ), attachment security and sibling relationship quality will be assessed. We anticipate that supportive sibling relationships will alleviate the adverse impacts of parental divorce on children's attachment security. This study will involve a sample of 80 siblings (40 sibling pairs) aged 7-17 years from divorced families. Findings may inform interventions enhancing attachment and resilience in children during family transitions, emphasizing sibling relationships as a valuable resource for well-being.

Exploring Intergenerational Attachment Transmission: How Are Children's Attachment Styles Affected By Changes In Their Caregiver’S Attachment?, Aisa Gibbons, Anahi Tapia

School of Psychology, Family, and Community Research Conference

Past studies have researched various connections between attachment styles of children and parents. However, no studies have explored how changing parents’ attachment style could impact change in children’s attachment style. This research proposal aims to investigate the relationship between caregiver attachment and child attachment change. We hope to recruit 100 parent-child pairings from schools and therapy clinics. Subjects will complete attachment measuring scales pre- and post parent-treatment. Parents will have 10-15 sessions of EFT to increase secure attachment. Attachment change differences will be analyzed through one-way ANOVA methods. We hypothesize that as parents’ secure attachment increases, their children’s will too.

Exploring The Impact Of The Blamer-Softening Change Event In Emotionally Focused Couples Therapy On Partners' Self-Esteem: A Longitudinal Intervention Study, Grace Lee

School of Psychology, Family, and Community Research Conference

It is no question that positive romantic relationships are connected to deep feelings of belonging, life satisfaction and positive self-esteem in individuals. However, throughout the course of the relationship, individuals can experience depressive symptoms, anxiety symptoms and negative affect. A significant marker of progress for couples in Emotionally Focused Couples Therapy is the presence of the blamer-softening change event. This event can help alleviate some of the negative symptoms partners experience. Given the well-accepted and understood importance of self-esteem on one’s overall mental health, this study seeks to understand the impact of the blamer-softening change event on each partner’s self-esteem.

Parenting Styles Of American And Filipino Mothers: Internal Family System’S Influence On Self-Leadership, Parent-Adolescent Communication, Conflict, Self-Esteem, And Depression, Maria-Sofia Paco, Gracie Cannon

School of Psychology, Family, and Community Research Conference

This abstract investigates the levels of Self-leadership among Filipino, European-American, and African-American mothers, and explores its implications for mother-daughter communication, conflict, depression, and overall self-esteem . Research has demonstrated that Filipino mothers have significantly higher mother-daughter communication and conflict compared to European-American and African-American mothers (Paco, 2007), positing a potential correlation between Filipino mothers (in the Philippines) and their Self-led nature compared to their European-American and African-American counterparts. Furthermore, this study proposes implementing Internal Family Systems (IFS) therapy as a means to enhance Self-leadership and improve mother-daughter communication and self-esteem across these three racial groups, potentially mitigating conflicts and depression. …

Group Narrative Therapy For Adolescents With Eating Disorders, Ramsey Ramsey

School of Psychology, Family, and Community Research Conference

Eating Disorders tend to be treatment-resistant, moreover, adolescents with eating disorders tend to disengage from standard treatments, such as (CBT-E) the standard intervention in treating eating disorders. Research shows that children and adults have greater positive outcomes with the addition of narrative approaches to treatment. Studies show that children and adults have positive outcomes with the addition of narrative approaches to treatment, in addition, group narrative therapy with adults is effective at treating eating disorders beyond behavioral change. This proposal is to explore the efficacy and client engagement of a group narrative therapy curriculum alongside standard CBT-E group treatments.

Multi-Class Emotion Classification With Xgboost Model Using Wearable Eeg Headband Data, James Khamthung, Nibhrat Lohia, Seement Srivastava

SMU Data Science Review

Electroencephalography (EEG) or brainwave signals serve as a valuable source for discerning human activities, thoughts, and emotions. This study explores the efficacy of EXtreme Gradient Boosting (XGBoost) models in sentiment classification using EEG signals, specifically those captured by the MUSE EEG headband. The MUSE device, equipped with four EEG electrodes (TP9, AF7, AF8, TP10), offers a cost-effective alternative to traditional EEG setups, which often utilize over 60 channels in laboratory-grade settings. Leveraging a dataset from previous MUSE research (Bird, J. et al., 2019), emotional states (positive, neutral, and negative) were observed in a male and a female participant, each for …

Unveiling The Influence Of Hyponatremia On Morbidity, Mortality, And Resource Utilization In Colorectal Carcinoma: Insights From A 5-Year Nationwide Study., Priya Hotwani Md, Vikash Kumar, Bhavyakumar Vachhani, Mrunanjali Gaddam, Jayalekshmi Jayakumar, Manasa Ginjupalli, Ankushi Sanghvi, Jincy Clement, Dhairya Nanavaty

Internal Medicine Residency

Meeting Abstract: 2024 ASCO Annual Meeting

Background: Historically, Hyponatremia has been associated with higher mortality rates and longer hospital stays in patients with solid tumors. The existing literature contains limited information on the connection between hyponatremia and Colorectal cancer (CRC). Thus, our study aimed to assess the impact of hyponatremia on mortality, morbidity, and resource usage in patients with CRC.

Methods: A retrospective analysis using the National Inpatient Sample database and International Classification of Diseases, Tenth Revision codes was conducted to examine the impact of hyponatremia on mortality, morbidity, and resource utilization in patients with CRC. Categorical variables were compared …

Zoom Dysmorphia: An Eye-Tracking Study Of Self-View And Attention During Video Conferences, Kathleen H. Stimson

Cognitive Science Senior Theses

This study investigates Zoom Dysmorphia, a heightened self-awareness and self-criticism of perceived physical flaws due to prolonged self-view on video conferencing platforms, with associated behaviors resembling symptoms of Body Dysmorphic Disorder (BDD). Drawing on Veale’s (2001, 2004) and Neziroglu’s (2004) cognitive-behavioral models of BDD and prior studies on BDD which suggest the development and maintenance of BDD through excessive self-focused attention and attentional bias, this study explores the potential cognitive and emotional implications of this phenomenon. Participants engaged in two mock video conferences with self-view enabled in one meeting and disabled the other for comparison. Eye tracking technology monitored their …

Closing The Communication Gap: Alignment Of Competency Performance Levels Between Ume And Gme, Anita Wilson, Phd, Katherine Berg, Md, Aaron Douglas, Phd, John Caruso, Md, Gretchen Diemer, Md, Kathleen Day, Ms, Rosemary Frasso, Phd, Steven Herrine, Md, David Abraham, Phd

JeffMD Academic Affairs

Purpose and Background

Graduate medical education (GME) program directors receive a minimal amount of information from the undergraduate medical education (UME) programs regarding an intern’s specific level of competence. This project’s purpose was to align the local undergraduate competency-based medical education program objectives (MEPOs) and GME outcomes measured during intern year. Previous studies show the gap in communication between UME and GME exists due to a lack of a shared mental model regarding competency performance.^{1, 2}

Triaging Your Health Concerns, James Bato Borja, Jasleen Mendoza, Addie Mccuistion, Jordan Artz, Julia Greeley, Ashley Diloreto

Nursing Leadership in Community Engagement Projects

Improving Health Literacy on Appropriate Use of Medical Services

As part of a nursing leadership project, a team of nursing students at Seattle Pacific University (SPU) collaborated with a Washington State affordable independent living facility for seniors; they focused on providing information on the appropriate use of emergency care, urgent care, and primary care services for various health concerns. This project began with a team member recognizing a pattern within the older adult population entering the Emergency Department (ED). This observation showed that a noticeable portion of the older adult population could benefit from clarification about when to seek out …

Generation And Characterization Of Lipid Nanoparticles For Dna Plasmid Delivery And Pharmacological Characterization, Sahana John

Biotechnology Theses

Lipid nanoparticles (LNPs) have shown promise for delivering nucleic acids like DNA plasmids, but packaging large plasmids remains challenging. The findings and experimental work detailed in this thesis are geared towards providing a proof of concept and providing preliminary confirmation of the guiding principle.

We synthesized LNPs containing cationic lipids (D-Lin-MC3) to encapsulate the large pLVX-ZsGreen and psiCHECK (luciferase) plasmids (~10-14kb) using a microfluidic system. Characterization included size, charge, morphology, plasmid encapsulation efficiency and in vitro/in vivo studies. The LNP-pDNA particles were ~100nm in size with high encapsulation efficiency. The LNPs efficiently transfected 293T cells with low cytotoxicity, enabling strong …

Coming Alongside Women Experiencing Homelessness, Alexis Dang, Brianna Cowan, Tashayla Braxton, Ellen Jacobs, Elizabeth Cortes, Erin Smith

Nursing Leadership in Community Engagement Projects

Coming Alongside Women Experiencing Homelessness

Executive Summary

Six nursing students attending Seattle Pacific University (SPU), partnered with a women’s day center to provide and support healthcare needs for a leadership project. During the first few weeks of being at the shelter, we visited the agency site to get a better understanding of the population and have a greater idea of what the focus problems should be. Our assessments consisted of a windshield survey, in-person observations, and conversations with the shelter director, staff, and women to identify barriers within their healthcare services. We further gathered assessment information from our instructor, who …

Early Outcomes Of Mr-Guided Sbrt For Patients With Recurrent Pancreatic Adenocarcinoma, Spencer Poiset, Sophia Shah, Louis Cappelli, Rani Anné, Karen Mooney, Maria Werner-Wasik, Talya Laufer, James Posey, Daniel Lin, Atrayee Mallick, Harish Lavu, Babar Bashir, Charles Yeo, Adam Mueller

Kimmel Cancer Center Faculty Papers

BACKGROUND: Local treatment options for locally recurrent pancreatic adenocarcinoma (LR-PAC) are limited, with median survival time (MST) of 9-13 months (mos) following recurrence. MRI-guided stereotactic body radiation therapy (MRgSBRT) provides the ability to dose escalate while sparing normal tissue. Here we report on the early outcomes of MRgSBRT for LR-PAC.

METHODS: Patients with prior resection of pancreatic adenocarcinoma with local recurrence treated with MRgSBRT at a single tertiary referral center from 5-2021 to 2-2023 were identified from our prospective database. MRgSBRT was delivered to 40-50 Gy in 4-5 fractions with target and OAR delineation per institutional standards. Endpoints included local …

Emotional Regulation On Modulating Associations Between Depression And Physical Activity As Characterized Via Deep Learning, Franklin Ye Ruan

Computer Science Senior Theses

Emotional regulation and physical activity are known to be associated with depression; however, a deeper understanding of how emotional regulation may strengthen or weaken the bonds between depressive symptoms and physical activity may aid clinicians and researchers in developing cognitive behavioral therapy (CBT) for those adversely affected by depression. As part of the Tracking Depression Study, this analysis uses data collected from 306 participants diagnosed with Major Depressive Disorder. To study their behavior, we analyze actigraphy data, or longitudinal physical activity intensity data, as it relates to depression severity, quantified by the daily PHQ-9 questionnaires. We study these associations through …

Socioeconomic Factors Influencing Hematological Health In Latin America, Lina M. Barrios, Ms

Student Papers, Posters & Projects

Hematological malignancies, also known as blood cancers, such as leukemia, lymphomas, and myelomas, are a significant health concern globally. The incidence of these malignancies has dramatically increased over time, leukemias being the leading cause. The incidence of blood cancers in Latin America exhibits regional variability, with leukemia being the emerging cause as the most prevalent type in children and young adults. However, the overall incidences are oddly lower than in developed countries, potentially due to the underreporting of the cases and the lack of cancer registries. This situation implies a critical need for improving surveillance and data collection methods to …

A Quantitative Study Examining The Mental Health Needs Of African American Students Compared To Their Counterparts In Higher Education, Luxury Bryant

College of Education Theses and Dissertations

African American students are entering into higher education institutions at higher rates. This population has specific needs and faces various challenges entering college at predominantly white institutions compared to their counterparts of different races. While college is already a stressful time with academic pressures as well as navigating a new setting, African American students also face unique experiences that can impact their mental health. It has been found that African American students are less likely to seek out mental health services especially those that are attending predominantly White institutions (Anderson, 2018). This study will highlight African American undergraduate students' perceptions …

Novel Role And Mechanisms Of Brd4 In Mediating Mesothelial To Mesenchymal Transition And Pleural Fibrosis, Felix Kankam

Biotechnology Theses

Pleural fibrosis is characterized by myofibroblast accumulation and increased expression of collagen and other extracellular matrices due to injury to the pleura and remodeling. Mesothelial to mesenchymal transition (MesoMT) is an important pathological event that contributes to the pool of myofibroblasts although its regulation remains incompletely understood. Recent research has reported the role of BRD4, a member of the bromodomain extra terminal domain (BET) family, in developing fibrosis in the heart and the lungs. One recent study showed that MEOX1 is a downstream target of BRD4 and mediated cardiac fibroblast activation. Our joint work also demonstrated that NOX4 is implicated …