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Articles 159121 - 159150 of 325133

Full-Text Articles in Medicine and Health Sciences

The Internationlization Of Sport Industry: A Study Of Thailand Sport Equipment Firms, Apithai Bumrungpanictarworn Jan 2020

The Internationlization Of Sport Industry: A Study Of Thailand Sport Equipment Firms, Apithai Bumrungpanictarworn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Entering international markets is one of many key strategies to ensure high and sustainable growth of the firm. It refers to how firms want to do business activities and their engagement in foreign markets. Many studies on the international market have been focused on entry mode strategies which is one of the most critical decisions in a firm's internationalization strategy. This research aimed to examine which factors influenced the entry mode decisions to enter foreign markets for sports equipment firms. A qualitative approach and multiple case study technique were used in this research. Important theories related to entry mode, such …

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Effects Of Social Interaction And Transformational Leadership On Sport Event Volunteers' Perception Of Team Member Exchange, Kasidech Treethong Jan 2020

Effects Of Social Interaction And Transformational Leadership On Sport Event Volunteers' Perception Of Team Member Exchange, Kasidech Treethong

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study contributes to the management of sport event volunteers, where particular team member exchange is crucial in promoting teamwork among sport event volunteers. The objectives of this study are 1) to examine the effects of social interaction and transformational leadership on the sport event volunteers' perception of team member exchange and 2) to examine the interaction effect between social interaction and transformational leadership on the sport event volunteers' perception of team member exchange. This study employed a field experiment and a post-experiment interview at the 2019 FIVB Volleyball Nations League Thailand. Subjects (n = 64) were assigned by stratified …

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Recovery Of Isolated Pancreatic Isletsderived From Gut Leak-Induced Diabetes Type Ii Mouse Model, Adretta Soedarmanto Jan 2020

Recovery Of Isolated Pancreatic Isletsderived From Gut Leak-Induced Diabetes Type Ii Mouse Model, Adretta Soedarmanto

Chulalongkorn University Theses and Dissertations (Chula ETD)

Gut leak in the obese patient was known to be one of the predisposing factors causing diabetes type II. Indeed, the interleukin-10 (IL-10) plays a key role in gut mucosa homeostasis preventing the gut leak. Nevertheless, the linkage of diabetes type 2 caused by gut leak with IL-10 deficiency in the non-diabetes patient has not been investigated yet. Islets transplantation is known as the best alternative treatment for diabetes. The culture of isolated islets is still facing difficulties to preserve the morphology, viability, and functionality. Therefore, the present study was aiming to prove the concept of gut-leak on IL-10 deficient …

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The Influence Of Sports Facilities' Accessibility, Motivation, And Satisfaction On Word-Of-Mouth And Re-Participation Intentions Of Athletes With Physical Disabilities, Thee Trongjitpituk Jan 2020

The Influence Of Sports Facilities' Accessibility, Motivation, And Satisfaction On Word-Of-Mouth And Re-Participation Intentions Of Athletes With Physical Disabilities, Thee Trongjitpituk

Chulalongkorn University Theses and Dissertations (Chula ETD)

More than a billion people are estimated to live with some form of disability, or about 15% of the world's population and the number is still growing. People with disabilities are less likely to participate in sports due to several physical barriers they face. In an effort to find ways to increase the sports participation rate of people with disabilities, the concept of marketing (satisfaction, word-of-mouth intention, and re-participation intention) was applied to the field of accessibility for the first time. This study consisted of three objectives: (1) to find out the dimension of sports facilities' accessibility and motivation, (2) …

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Effect Of Dietary Microencapsulated Organic Acid And Essential Oil On Sow Performance And Nursery Piglet Growth Performance And Fecal Bacteria, Aprilia Rizky Riadini Jan 2020

Effect Of Dietary Microencapsulated Organic Acid And Essential Oil On Sow Performance And Nursery Piglet Growth Performance And Fecal Bacteria, Aprilia Rizky Riadini

Chulalongkorn University Theses and Dissertations (Chula ETD)

The effect of feed supplementation with microencapsulated organic acids and essential oils (MOE, Porcinate+) in feed was investigated according to sow and nursery pig growth performances, and fecal bacteria population. Experiment 1: healthy three hundred and twenty sows during its late gestation period (7 days before farrowing) until the weaning phase (28 days after farrowing) were randomly divided as control group (160 sows) which was fed with basal diet, and treatment group (160 sows), which was fed with basal diets supplemented with 2 kilograms (kg) of MOE in 1 ton of feed. Each of 4 replications was composed of 40 …

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Porcine Reproductive And Respiratory Syndrome Virus: Genetic Diversity, Pathogenesis And Modified-Live Vaccines, Adthakorn Madapong Jan 2020

Porcine Reproductive And Respiratory Syndrome Virus: Genetic Diversity, Pathogenesis And Modified-Live Vaccines, Adthakorn Madapong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine reproductive and respiratory syndrome virus (PRRSV) is a causative agent of PRRS that reproductive failure in sows and respiratory problems in piglets is the hallmark of the disease. Recently, several PRRSV modified live vaccines (MLV) are available, and vaccine selection is a concern. Therefore, the objectives of this study were: 1) to investigate the genetic diversity of Thai PRRSV isolates during 2001-2017; 2) to evaluate the pathogenicity of Thai PRRSV isolates; 3) to determine the efficacy of PRRSV MLV against Thai field PRRSV infection and 4) to investigate the effectiveness of PRRSV MLV when administered via intramuscular (IM) and …

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Genetic Characteristics Of Antimicrobial Resistance In Escherichia Coli Isolated From Pigs, Pork And Humans In Thailand And Lao Pdr Border Provinces, Chanika Pungpian Jan 2020

Genetic Characteristics Of Antimicrobial Resistance In Escherichia Coli Isolated From Pigs, Pork And Humans In Thailand And Lao Pdr Border Provinces, Chanika Pungpian

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of this study were to monitor the prevalence of antimicrobial resistance (AMR) among Escherichia coli isolated from pigs, pork and humans in Thailand-Laos border areas, characterize AMR of E. coli isolated from pigs, pork and humans in Thailand-Laos border areas, and compare the resistance to last-line antimicrobials of E. coli and Salmonella isolated from pigs and pork in Thailand-Laos and Thai-Cambodia border areas. Three study projects were conducted. Project 1 demonstrated the results of phenotypic and genotypic monitoring of AMR in E. coli in pigs, pork, and humans in Thailand and Lao PDR border provinces. A total of …

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Microbiological Quality Of And Antimicrobial Resistance In Commercial Probiotic Products For Food Animals In Thailand, Hoang My Tran Jan 2020

Microbiological Quality Of And Antimicrobial Resistance In Commercial Probiotic Products For Food Animals In Thailand, Hoang My Tran

Chulalongkorn University Theses and Dissertations (Chula ETD)

A total of 45 commercial probiotic products for food animals were investigated for the number of viable cells, bacterial species and the presence of antimicrobial resistance (AMR) genes. All products were enumerated for viable bacterial cells of Lactobacillus, Bacillus and Enterococcus. Confirmation of species was carried out by multiplex Polymerase Chain Reaction (PCR) for Lactobacillus (n=20), Enterococcus (n=20), and Amplified Ribosomal DNA Restriction Analysis (ARDRA) for Bacillus (n=190). The presence of Clostridium species was examined by PCR. The contamination of E. coli and Salmonella was also determined. Minimal Inhibitory Concentrations (MICs) for 14 antimicrobials was examined in the bacterial isolates …

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Localization Of Tilapia Tilapinevirus By In Situ Hybridization In The Brain Of Nile Tilapia (Oreochromis Niloticus), Hung Dinh Nguyen Jan 2020

Localization Of Tilapia Tilapinevirus By In Situ Hybridization In The Brain Of Nile Tilapia (Oreochromis Niloticus), Hung Dinh Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Tilapia tilapinevirus or tilapia lake virus (TiLV) is an emerging virus that poses a significant negative impact on farmed tilapia globally. Abnormal behaviours such as lethargy, loss of appetite, erratic swimming, stop schooling have been recorded during disease occurrence, which is possibly linked to brain activities. Previous studies described detection of the virus in multiple organs of infected fish; however, little is known about the in-depth localization of the virus in the central nervous system. Herein, we determined the distribution of TiLV and histopathological alterations in the entire brain of Nile tilapia experimentally infected fish. By in situ hybridization (ISH) …

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Detection Of Indicator And Pathogenic Bacteria In Raw Vegetables And Fruits Sold In Fresh Markets And Supermarkets, Bangkok, Thailand, Jutanat Srisamran Jan 2020

Detection Of Indicator And Pathogenic Bacteria In Raw Vegetables And Fruits Sold In Fresh Markets And Supermarkets, Bangkok, Thailand, Jutanat Srisamran

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to compare the concentrations of fecal coliforms and Escherichia coli, and the contamination of Salmonella, Shigella, E. coli O157: H7 and Listeria monocytogenes in raw vegetables and fruits between fresh markets and supermarkets in Bangkok, Thailand. A total of 405 produce samples, consisting of sweet basil, spring onion, coriander, cabbage, lettuce, cucumber and tomato, were randomly collected from seven fresh markets (n=203) and 29 supermarkets (n=202) in seven districts of Bangkok between June 2018 and July 2019. T-test and one-way ANOVA were used to compare the concentrations of fecal coliforms and E. coli (log10MPN/g) between fresh markets …

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A Study Of Using Corncob, Aspen Shaving, Dried Water Hyacinth And Banana Midrib As Bedding For Laboratory Mice, Nantaporn Maytayapirom Jan 2020

A Study Of Using Corncob, Aspen Shaving, Dried Water Hyacinth And Banana Midrib As Bedding For Laboratory Mice, Nantaporn Maytayapirom

Chulalongkorn University Theses and Dissertations (Chula ETD)

A study of using corncob, aspen shaving, dried water hyacinth and banana midrib as bedding for laboratory mice. The objective of this study was to evaluate the physical characteristics (i.e., absorbency and surface moisture), the physiological effects and intracage ammonia level of 4 types of beddings in laboratory mice. This study was divided into two parts. In the first part, the fluid absorption properties of these four beddings were studied using measurement of liquid absorption and the surface moisture. The results showed that the volumetric absorbency of aspen shaving was significantly higher than other beddings. While, the surface moisture showed …

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The Study Of Changes In Gene Regulation Of Serotonin Signaling Pathway And Circulating Serotonin Concentration In Dogs With Pulmonary Hypertension Due To Degenerative Mitral Valve Disease, Nattawan Tangmahakul Jan 2020

The Study Of Changes In Gene Regulation Of Serotonin Signaling Pathway And Circulating Serotonin Concentration In Dogs With Pulmonary Hypertension Due To Degenerative Mitral Valve Disease, Nattawan Tangmahakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Pulmonary hypertension (PH) is an abnormal increase in pulmonary vascular pressure. In dogs, PH is commonly found secondary to degenerative mitral valve disease (DMVD), especially in small breed dogs. Serotonin, a biogenic amine playing an essential role in both physiology and abnormalities of several organs, is a vasoactive substance that has been one of the suspicious mediators for the development of PH. Both local and circulating effects of serotonin have been investigated to discover the involvement of serotonin and the pathogenesis of PH in both humans and animals. The present study aimed to investigate the local serotonin signaling in lung …

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Targeted Surveillance Of Potential Zoonotic Respiratory And Enteric Viruses In Dogs In Bangkok, Thailand, Kamonpan Charoenkul Jan 2020

Targeted Surveillance Of Potential Zoonotic Respiratory And Enteric Viruses In Dogs In Bangkok, Thailand, Kamonpan Charoenkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Human-dog interface poses a risk of transmission and spread of zoonotic viruses. The objective of the thesis entitled is to survey and genetic characterize zoonotic viruses in dogs and humans who have high-risk occupations and in close contact with dogs. This thesis contains 8 topics. The results of the first to third topics were the surveillance of respiratory viruses in dogs including Canine Parainfluenza type 5 (CPIV-5), Canine influenza virus (CIV) and Coronavirus (CoV). The nasal swab samples were collected from dogs with respiratory signs during 2015-2018. The results showed that the occurrence of CPIV-5, CIV and CoV were 5.6% …

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Comparison Of Laser Class 1m And Class 4 Applications In Canine Cranial Cruciate Ligament Rupture Evaluated By Clinical Outcomes And Ultrasonography, Noppakrit Ekakaraworapong Jan 2020

Comparison Of Laser Class 1m And Class 4 Applications In Canine Cranial Cruciate Ligament Rupture Evaluated By Clinical Outcomes And Ultrasonography, Noppakrit Ekakaraworapong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Nineteen dogs diagnosed of cranial cruciate ligament rupture were included in this study before TPLO was performed. They were classified into 3 groups which consisted of control, low-level laser therapy class 1M and low-level laser therapy class 4 groups. The number of each group were 4, 8 and 7 dogs, respectively, and the duration of treatment protocol was 4 weeks consecutively. On the first day of the study, dogs were examined and assessed of the clinical signs such as lameness score, synovial fluid analysis, canine brief pain inventory questionnaire (CBPI) and ultrasonographic score. In ultrasonographic monitoring, ultrasonographic scores on synovial …

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Efficiency And Clinical Outcomes Of Platlet-Rich Plasma Therapy On Caninecoxofemoral Osteoarthritis, Patama Arunrattanakul Jan 2020

Efficiency And Clinical Outcomes Of Platlet-Rich Plasma Therapy On Caninecoxofemoral Osteoarthritis, Patama Arunrattanakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

The purpose of this study was to find out for the optimal technique to prepare plasma with highest platelet concentration in platelet rich plasma (PRP), as well as determine the result of autologous PRP from the dogs with osteoarthritis(OA) condition. It was shown that the PRP could reduce inflammation, cell regeneration, and enhance angiogenesis to support the injured tissue. This study was composed of two phases; the first phase was aimed to find the best centrifugation speed and time that could be able to produce the plasma with the highest platelet concentration by conducting the experiment from a total of …

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Surveillance Of Coronaviruses In Fruit Bats (Pteropus Lylei) Of Thailand, Pichamon Moolthep Jan 2020

Surveillance Of Coronaviruses In Fruit Bats (Pteropus Lylei) Of Thailand, Pichamon Moolthep

Chulalongkorn University Theses and Dissertations (Chula ETD)

Coronavirus (CoV) is an enveloped single positive-stranded RNA virus CoV can cause enteric and respiratory diseases in mammals, avian and human. Bats are the natural reservoir of CoVs and distribute all over Thailand. Sample collection was performed in two bat colonies in Ayutthaya and Saraburi provinces during March 2018 - February 2019. Total 1,487 samples were collected from 730 bats. The bat species identification via morphological measurement and cytochrome B detection showed that bats in both colonies are Lyle's flying fox (Pteropus lylei). The results of CoV detection showed that 4.30% of samples were positive to CoV. Phylogenetic analysis showed …

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Effects Of Algae-Derived Ss-Glucan Supplementation On Production Performances And Specific Immune Responses In Nursery To Growing Pigs, Sh.Boonnithi Chuaychu Jan 2020

Effects Of Algae-Derived Ss-Glucan Supplementation On Production Performances And Specific Immune Responses In Nursery To Growing Pigs, Sh.Boonnithi Chuaychu

Chulalongkorn University Theses and Dissertations (Chula ETD)

เบต้ากลูแคนเป็นสารเสริมในอาหารสัตว์ที่มีการใช้กันอย่างแพร่หลายในอุตสาหกรรมการเลี้ยงสุกร ถึงแม้ว่าการเสริมสารเบต้ากลูแคนในสูตรอาหารสุกร จะเชื่อว่าสามารถช่วยพัฒนาการเจริญเติบโตและปรับการทำงานของภูมิคุ้มกันในสุกรได้ แต่อย่างไรก็ตาม ในปัจจุบันยังไม่มีข้อมูลการศึกษาที่ชัดเจนเกี่ยวกับผลของการเสริมเบต้ากลูแคนที่สกัดมาจากสาหร่ายต่ออัตราการเจริญเติบโตและการตอบสนองทางภูมิคุ้มกันโดยเฉพาะระบบภูมิคุ้มกันแบบจำเพาะในสุกร ดังนั้นการศึกษานี้จึงมีวัตถุประสงค์เพื่อประเมินผลของการใช้สารเสริมเบต้ากลูแคนจากสาหร่ายในอาหารสุกรต่อประสิทธิภาพการเจริญเติบโตและการตอบสนองทางภูมิคุ้มกันแบบจำเพาะในสุกรอนุบาลและสุกรขุนเล็ก โดยแบ่งสุกรหย่านมสามสายพันธุ์เป็น 3 กลุ่มการทดลอง ได้แก่ กลุ่มควบคุมที่ได้รับอาหารสูตรมาตรฐาน กลุ่มทดลองที่ 2 และ 3 ได้รับอาหารชนิดเดียวกันที่เสริมด้วยสารเสริมเบต้ากลูแคนจากสาหร่ายในปริมาณ 100 และ 200 กรัมต่อตันตามลำดับ จากการทดลอง ไม่พบความแตกต่างอย่างมีนัยสำคัญของประสิทธิภาพการผลิตและอัตราการเจริญเติบโตของสุกรสุกร การทดสอบการตอบสนองของระบบภูมิคุ้มกันที่จำเพาะหลังการฉีดวัคซีน CSFV และ PRRSV พบว่าสุกรที่ได้รับสารเสริมเบต้ากลูแคนจากสาหร่ายในอาหาร มีระดับ neutralizing antibody ต่อ CSFV สูงขึ้นอย่างมีนัยยะสำคัญ นอกจากนี้ พบการเพิ่มขึ้นของจำนวนเซลล์ CD4+(T lymphocytes) และ CD4+CD8+ (putative memory T lymphocytes) ซึ่งสร้างไซโตคายน์ IFN-g+ ที่มีความจำเพาะต่อ CSFV และ PRRSV ในกลุ่มสุกรที่ได้รับสารเสริมเบต้ากลูแคนจากสาหร่ายอีกด้วย จากการศึกษาสรุปได้ว่า ถึงแม้ว่าการให้สารเสริมเบต้ากลูแคนจะไม่สามารถเสริมสมรรถภาพการผลิตของสุกรได้ แต่มีคุณสมบัติทางชีวภาพในการเป็นสารกระตุ้นภูมิคุ้มกันซึ่งอาจเป็นการเพิ่มความต้านทานโรคติดเชื้อไวรัสในสุกรอนุบาลและสุกรขุนเล็กได้

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Development Of Conventional And Taqman Real-Time Polymerase Chain Reaction Methods For Detection Of Liver Fluke Infection With Platynosomum Spp. In Cat Feces, Shane Thiha Soe Jan 2020

Development Of Conventional And Taqman Real-Time Polymerase Chain Reaction Methods For Detection Of Liver Fluke Infection With Platynosomum Spp. In Cat Feces, Shane Thiha Soe

Chulalongkorn University Theses and Dissertations (Chula ETD)

The Platynosomum spp. fluke is the etiologic agent of platynosomiasis responsible for hepatobiliary diseases in cats. Coprological examination using centrifugal sedimentation technique is the gold standard method for detection of Platynosomum spp.'s eggs. However, microscopic examination method has a limited sensitivity, particularly in cases of complete biliary obstruction and inconsistent egg shedding. To circumvent these problems, this study aims to develop molecular-based methods for detecting Platynosomum spp. infection in cat feces including conventional PCR (cPCR) and TaqMan real-time PCR methods targeting ITS1 region. From a total number of 120 cat fecal samples, when compared to microscopic examination, the cPCR method …

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Surveillance And Histopathological Study Of Microplastics In Marine Fish From The Gulf Of Thailand, Sirawich Srisiri Jan 2020

Surveillance And Histopathological Study Of Microplastics In Marine Fish From The Gulf Of Thailand, Sirawich Srisiri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Microplastic (MP) problems have been threatened aquatic environment worldwide for decades. This study demonstrated current MP contamination characteristics in marine food fishes from the upper Gulf of Thailand. MPs were found in gastrointestinal tracts of 46.86% of fish samples, which were 1.556±0.47 pieces per fish or 0.035±0.014 pieces per gram of fish bodyweight. No plastics were detected from muscle, liver, kidney or gonad of the fishes. There was a significant relationship between MP contamination and fish species or the fish grouping. Benthic fish had a higher contamination rate than pelagic fish. Fiber-type and blue color were the most abundant MPs …

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The Effect Of Maternally-Derived Antibodies Against Antibody Production In Vaccinated Piglets With Modified-Live Porcine Reproductive And Respiratory Syndrome Virus Vaccines, Sunit Mebumroong Jan 2020

The Effect Of Maternally-Derived Antibodies Against Antibody Production In Vaccinated Piglets With Modified-Live Porcine Reproductive And Respiratory Syndrome Virus Vaccines, Sunit Mebumroong

Chulalongkorn University Theses and Dissertations (Chula ETD)

The humoral antibody responses following vaccination with different lineage of modified live genotype 2 porcine reproductive and respiratory syndrome virus (PRRSV) vaccines (MLVs) were investigated. Moreover, the mortality and growth performance in pigs were observed. Twenty-four pigs with high maternally derived antibody (MDA) levels (SN titer ≥ 8) from each PRRSV positive Herd 1 and Herd 2 were allocated to the H1, H1-1, and H1-2 groups (Herd 1) and H2, H2-1, and H2-2 groups (Herd 2). Twenty-four pigs with low MDA levels (SN titer <8) were allocated to the L1, L1-1, and L1-2 (Herd 1) and L2, L2-1, and L2-2 groups (Herd 2). Twenty-four pigs from the negative herd (Herd 3) were allocated to 3 groups: N, N-1, and N-2 groups. The H1, H2, L1, L2, and N groups were unvaccinated. The H1-1, H2-1, L1-1, L2-1, and N-1 groups were vaccinated with MLV (Ingelvac® PRRS MLV) at 2 weeks of age. The 5 other groups: H1-2, H2-2, L1-2, L2-2, and N-2 groups were vaccinated with MLV2 (Prime Pac® PRRS MLV) at 2 week of age. The antibody responses were measured by ELISA and serum neutralization (SN). The mortality was not observed in all vaccinated groups. Additionally, pigs with high MDA levels from the H1 and H2 groups did not exhibit the mortality throughout the study. ADG in pigs vaccinated with each MLV1 and MLV2 was significant higher than that in unvaccinated pigs. However, pigs vaccinated with MLV2 showed the highest values of ADG. Seroconversion was observed in all vaccinated groups with no significance difference in antibody titers between pigs with low and high MDA levels. However, pigs with low MDA levels showed the earliest and highest antibody level post vaccination. The highest SN-titer against MLV1 isolate and MLV2 isolate was observed in pig from the L1-1 and L2-2 groups, respectively. The field PRRSV-2 isolates: THA_SP/RB_S1/P1/0120-18 and THA_WC/RB_F165/20-22, the SN titers against these isolates were not observed in vaccinated groups. These results indicated that MDAs might not affect the immune response post vaccination. PRRSV-2 MLVs reduced the mortality and improved growth performance of pigs in the endemically PRRSV infected herds. Although, the vaccine was changed in weaning pigs, the antibody responses were detected post vaccination in both pigs with low and high level of MDAs. Moreover, the genetic similarity between vaccine and field virus was not related to the protection. Vaccine selection should depend on the induction of immune response and protection against heterologous PRRSV infection.

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Genetic Characteristics Of Antimicrobial Resistance Of Salmonella Enterica And Escherichia Coli Isolated From Broilers, Pigs And Their Meat Products In Sa Kaeo, Banteay Meanchey And Siem Reap Provinces, Suthathip Trongjit Jan 2020

Genetic Characteristics Of Antimicrobial Resistance Of Salmonella Enterica And Escherichia Coli Isolated From Broilers, Pigs And Their Meat Products In Sa Kaeo, Banteay Meanchey And Siem Reap Provinces, Suthathip Trongjit

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research study aimed to investigate the incidence and characterize antimicrobial resistance (AMR) in Salmonella enterica and Escherichia coli isolated from broilers, pigs and their meat products in Thailand–Cambodia border provinces. Resistance to clinically importance antimicrobials was additionally examined in the E. coli isolates from other provinces with high pig production in Thailand. A total of 941 samples were collected from broilers and pigs at slaughterhouses and their carcasses at local fresh markets in Sa Kaeo, Thailand (n=554) and Banteay Meanchey and Siem Reap, Cambodia (n=387) during 2014-2015. Three hundred forty-five Salmonella isolates were collected from Thailand (n = 145; …

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Detection And Genetic Characterization Of Emerging And Re-Emerging Viral Enteric Diseases In Swine In Thailand, Taveesak Janetanakit Jan 2020

Detection And Genetic Characterization Of Emerging And Re-Emerging Viral Enteric Diseases In Swine In Thailand, Taveesak Janetanakit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Emerging and re-emerging important enteric viruses in pigs including porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV) and enterovirus G (EVG) are important pathogens of food security and public health concerns. In Thailand, the information on the occurrences and status of PEDVs, PDCoVs and EVGs is limited. Especially, Thai-EVGs have never been reported before. This thesis composes of three study phases. Phase 1 is surveillance of swine enteric viruses in pig farms. Phase 2 is genetic characterization and phylogenetic analysis of swine enteric viruses. Phase 3 is development of rapid diagnostic tests using RT-LAMP with lateral flow device (LFD) and …

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Bacteriophage And Ozone-Nanobubble Based Approaches For Combating Multidrug-Resistant Aeromonas Hydrophila In Nile Tilapia (Oreochromis Niloticus) Culture System, Thanh Dien Le Jan 2020

Bacteriophage And Ozone-Nanobubble Based Approaches For Combating Multidrug-Resistant Aeromonas Hydrophila In Nile Tilapia (Oreochromis Niloticus) Culture System, Thanh Dien Le

Chulalongkorn University Theses and Dissertations (Chula ETD)

Aeromonas hydrophila infection is considered one of the most important bacterial diseases responsible for the loss of millions of dollars in the global freshwater aquaculture industry. Multidrug-resistant (MDR) A. hydrophila is becoming a global issue of concern. Non-antibiotic approaches can minimize the requirement for antimicrobials to combat infectious diseases and antimicrobial resistance. This study aims to investigate the potential application of lytic bacteriophage, ozone nanobubbles (NB-O3), a potential combination of bacteriophage and nanobubble-based technology in combatting MDR A. hydrophila in Nile tilapia. A newly Myoviridae phage pAh6.2TG was isolated, identified, and characterized in this study. The application of this phage …

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Relationship Between Insulin-Like Growth Factor-1 And Semen Quality In Asian Elephants (Elephas Maximus), Yuqing Yang Jan 2020

Relationship Between Insulin-Like Growth Factor-1 And Semen Quality In Asian Elephants (Elephas Maximus), Yuqing Yang

Chulalongkorn University Theses and Dissertations (Chula ETD)

Evidence has been increasingly suggested that insulin-like growth factor-1 (IGF-1) is related to steroidogenesis and spermatogenesis. This may be conducive to find the causes of poor sperm quality in Asian elephants (Elephas maximus). The present study aimed to investigate whether correlations among serum IGF-1 concentration, serum testosterone level and semen variables exist in elephants. A total of 17 ejaculates (1–3 ejaculates/bull) were collected by performing transrectal massage. Before each ejaculate, blood samples were obtained (n = 17). Subsequently, semen characteristics of each ejaculate were evaluated. Assessments of precision (calculations of the intra- and inter-assay coefficient of variation, CV) and accuracy …

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Toe Clearance And Lower Limb Kinematics During Swing Phase Of Walking Over The Obstacles In Postoperative Total Knee Arthroplasty, Archrawadee Srijaroon Jan 2020

Toe Clearance And Lower Limb Kinematics During Swing Phase Of Walking Over The Obstacles In Postoperative Total Knee Arthroplasty, Archrawadee Srijaroon

Chulalongkorn University Theses and Dissertations (Chula ETD)

The purpose of this study was to determine minimum toe clearance and lower limb kinematics at initial swing and mid-swing phase during walking over the obstacles in patients with knee osteoarthritis after total knee arthroplasty (TKA). Twenty patients aged 65-85 years with knee OA were included to the study before surgery, three and six months following TKA and twenty age-matched healthy controls were collected the data for comparisons. All participants were asked to perform range of motion test, muscle length test, leg length, leg muscles strength test, joint position sense test, single-leg balance test, Knee Injury Osteoarthritis Outcome Score (KOOS). …

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Study Of Platelet Production From Megakaryocyte By Using Induced Pluripotent Stem Cell, Jaturawat Pawinwongchai Jan 2020

Study Of Platelet Production From Megakaryocyte By Using Induced Pluripotent Stem Cell, Jaturawat Pawinwongchai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Thrombopoiesis is the process of platelet production from hematopoietic stem cells (HSCs). Glycoproteins (GP) Ib-IX-V that is expressed on the surface of megakaryocytes and platelets binds von Willebrand factor (VWF) plays roles in platelet production. Either the GPIb deficiency or hyper-function can cause macrothrombocytopenia, the molecular mechanisms remain unclear. In this study, the pathogenesis investigations were performed in the human induced pluripotent stem cell (hiPSC) model. CRISPR-Cas9 was used to generate the hiPSCs carrying a gain-of-function GP1BA p.M255V mutation which was described in platelet-type von Willebrand disease (PT-VWD). The GPIb deficiency hiPSCs were previously derived from a Bernard Soulier syndrome …

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Effect Of Ovalitenone On Non-Small Cell Lung Cancer Cells, Kittipong Sanookpan Jan 2020

Effect Of Ovalitenone On Non-Small Cell Lung Cancer Cells, Kittipong Sanookpan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Cancer metastasis is the major cause of about 90% of cancer deaths. As epithelial-to-mesenchymal transition (EMT) is known for potentiating metastasis. In the present study aimed to elucidate the effect of ovalitenone on the suppression of EMT and metastasis-related behaviors, including cell movement and growth under detached conditions, and cancer stem cells (CSCs), of lung cancer cells. Ovalitenone, as a flavonoid was extracted from roots of Millettia erythrocalyx Gapnep., was the first reported to show the anti-migration activity of H460 and A549 lung cancer cells. Ovalitenone was used at concentrations of 0–200 μM. While it caused no cytotoxic effects on …

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Microencapsulation Of Black Cumin Seed Oil With Hydrocolloids Via Spray Drying, Najwa Yanya Santiworakun Jan 2020

Microencapsulation Of Black Cumin Seed Oil With Hydrocolloids Via Spray Drying, Najwa Yanya Santiworakun

Chulalongkorn University Theses and Dissertations (Chula ETD)

Nigella sativa is one of historical and miracle herbs been used for many centuries in different civilizations. Although its oil provides high nutritious values of fatty acids and phytochemicals, the strong odor and peppery taste limits its application in food products. In additions, the black cumin seed oil also poor solubility and rapid deterioration. A wise strategy to enhance organoleptic property and promote oil solubility is encapsulating the cold press black cumin seed oil with suitable wall materials via spray drying. In this study, eight water soluble hydrocolloids were used for formulating the mixed microcapsules. Only four formulations of microcapsules …

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Synergistic Activities Of Antibiotic Combinations Against Carbapenem-Resistant Klebsiella Pneumoniae And Mechanisms Of Carbapenem Resistance In Klebsiella Pneumoniae Clinical Isolates., Netchanok Muhummudaree Jan 2020

Synergistic Activities Of Antibiotic Combinations Against Carbapenem-Resistant Klebsiella Pneumoniae And Mechanisms Of Carbapenem Resistance In Klebsiella Pneumoniae Clinical Isolates., Netchanok Muhummudaree

Chulalongkorn University Theses and Dissertations (Chula ETD)

Carbapenem-resistant Klebsiella pneumoniae (CRKP) has been increasing reported worldwide. The aims of this study were to investigate the mechanisms of carbapenem resistance in K. pneumoniae Thai isolates and study the synergistic activities of antibiotic combinations against CRKP isolates. Two-hundred and forty CRKP clinical isolates were obtained from patients at King Chulalongkorn Memorial Hospital between September 2016 and April 2020. The antibiotic susceptibility testing was determined by agar dilution to imipenem, meropenem, amikacin, fosfomycin, ciprofloxacin and by broth microdilution to colistin. The prevalence rates of resistance to imipenem, meropenem, ciprofloxacin, fosfomycin, amikacin, and colistin were 90.42%, 91.67%, 95.84%, 41.25%, 20%, and …

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Application Of Phage Display Technology For The Production Of Antibodies Againststreptococcus Suis Serotype 2, Pattarawadee Sulong Jan 2020

Application Of Phage Display Technology For The Production Of Antibodies Againststreptococcus Suis Serotype 2, Pattarawadee Sulong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Streptococcus suis (S. suis) serotype 2 infection is problematic in the swine industry and responsible for most cases of human infection worldwide. Since current multiplex PCR cannot differentiate between serotypes 2 and 1/2, then serotype-specific antibodies (Abs) are required for serotype identification to confirm S. suis serotype 2 infection. This study aimed to generate Abs specific to S. suis serotype 2 by phage display from a human heavy chain variable domain (VH) antibody library. For biopanning, whole cells of S. suis serotype 2 were used as the target antigen. With increasing selection stringency, we could select the VH Abs that …

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