Medicine and Health Sciences Commons^™

The Sineb1 Element In The Long Non-Coding Rna Malat1 Is Necessary For Tdp-43 Proteostasis, Tuan M Nguyen, Elena B Kabotyanski, Lucas C Reineke, Jiaofang Shao, Feng Xiong, Joo-Hyung Lee, Julien Dubrulle, Hannah Johnson, Fabio Stossi, Phoebe S Tsoi, Kyoung-Jae Choi, Alexander G Ellis, Na Zhao, Jin Cao, Oluwatoyosi Adewunmi, Josephine C Ferreon, Allan Chris M Ferreon, Joel R Neilson, Michael A Mancini, Xi Chen, Jongchan Kim, Li Ma, Wenbo Li, Jeffrey M Rosen

Faculty, Staff and Students Publications

Transposable elements (TEs) comprise a large proportion of long non-coding RNAs (lncRNAs). Here, we employed CRISPR to delete a short interspersed nuclear element (SINE) in Malat1, a cancer-associated lncRNA, to investigate its significance in cellular physiology. We show that Malat1 with a SINE deletion forms diffuse nuclear speckles and is frequently translocated to the cytoplasm. SINE-deleted cells exhibit an activated unfolded protein response and PKR and markedly increased DNA damage and apoptosis caused by dysregulation of TDP-43 localization and formation of cytotoxic inclusions. TDP-43 binds stronger to Malat1 without the SINE and is likely 'hijacked' by cytoplasmic Malat1 to the …

Discovery Of A Bacterial Peptide As A Modulator Of Glp-1 And Metabolic Disease, Catherine Tomaro-Duchesneau, Stephanie L Levalley, Daniel Roeth, Liang Sun, Frank T Horrigan, Markus Kalkum, Joseph M Hyser, Robert A Britton

Faculty, Staff and Students Publications

Early work in rodents highlighted the gut microbiota's importance in metabolic disease, including Type II Diabetes Mellitus (T2DM) and obesity. Glucagon-like peptide-1 (GLP-1), an incretin secreted by L-cells lining the gastrointestinal epithelium, has important functions: promoting insulin secretion, insulin sensitivity, and β-cell mass, while inhibiting gastric emptying and appetite. We set out to identify microbial strains with GLP-1 stimulatory activity as potential metabolic disease therapeutics. Over 1500 human-derived strains were isolated from healthy individuals and screened for GLP-1 modulation by incubating bacterial cell-free supernatants with NCI H716 L-cells. Approximately 45 strains capable of increasing GLP-1 were discovered. All GLP-1 positive …

Using Active Learning To Build A Foundation For Bioinformatics Training., Stacey E. Wahl Ph.D., Amy L. Olex Ms

Transforming Libraries for Graduate Students

As Health Sciences Libraries evolve, the support they offer graduate students has evolved to incorporate many aspects of the research life cycle. At Tompkins-McCaw Library for the Health Sciences, we have partnered with the Wright Center for Clinical and Translational Research to offer training workshops for graduate students who are interested in using bioinformatics to plan, analyze, or execute scientific experiments. We offer two series: 1) an 8-week, 1-hour per week seminar series providing a general overview of available techniques and 2) a week-long intensive, two hours per session, series on utilizing free databases from the National Center for Biotechnology …

Helping Stem Graduate Students Fall Into Research, Diana Hartle, Kelsey Forester

Transforming Libraries for Graduate Students

Each Fall and Spring semester, the UGA Science Library hosts a series of workshops for undergraduate and graduate students during one consolidated week focused on research needs. In the past year, librarians at the Science Library noticed a large and growing need for research and wellness support for our STEM graduate students. This led us to begin to collaborate with the graduate school, University Health Center, and other science and medical librarians. Through this collaboration, we reconstructed our semesterly workshop series to be tailored specifically to STEM graduate students. We offered workshops on citation management, tools for tracking scholarly presence, …

Managing Uncertainty In Chronic Illness From Patient Perspectives, Amanda Brown, Susan Hayden, Karen Klingman, Leslie C. Hussey

Journal of Excellence in Nursing and Healthcare Practice

Chronic illness is an increasing concern in the United States as the Centers for Disease Control and Prevention reports that 50% of adults have at least one chronic illness. When individuals must adjust to chronic illness, they may experience uncertainty regarding the illness, prognosis, and symptoms. The purpose of this qualitative, phenomenological study was to explore the management strategies of uncertainty in chronic illness from patients’ perspectives. Merle Mishel’s uncertainty in illness theory was the framework used to explore the lives of individuals diagnosed and living with chronic illness. In-depth interviews were conducted with eight individuals who have chronic disease. …

Gallagher Bluedorn And Unitix Box Offices Closed, March 17, 2020, University Of Northern Iowa

UNI Response to COVID-19

A memo announcing the closure of the Gallagher-Bluedorn Performing Arts Center and the UNI tickets office.

Gov. Reynolds Issues A State Of Public Health Disaster Emergency, March 17, 2020, University Of Northern Iowa

UNI Response to COVID-19

A press release from the Governor declaring a state of public health disaster emergency, including the text of the proclamation.

2020 March 17 - Medical Student Education Committee Minutes, Medical Student Education Committee, East Tennessee State University

Medical Student Education Committee Minutes

No abstract provided.

2020-03-17_Michiganoverview_Covidstats, Michigan Department Of Health & Human Services

Cumulative Michigan COVID-19 Data

The COVID Overview page was obtained from Michigan's main COVID-19 page: https:/www.michigan.gov/coronavirus/0,9753,7-406-98163-520743--,00.html. Data was compiled by the Michigan Department of Health and Human Services (MDHHS). The information was updated daily at 2 p.m., with yesterday’s COVID-19 positive results included as of midnight.

The website contained the tables Confirmed COVID-19 Cases by County, Percentage of Cases by Sex, Percentage of Cases by Age, and Hospitalization.

A Literature-Based Approach To Morphological Awareness Intervention, Morgan Stone

Student Theses and Dissertations

This study was designed to assess the effectiveness of a literature-based approach to a morphological awareness intervention on the literacy and language skills of a fourth-grade child with a language impairment. One child, diagnosed with a language impairment, participated in this study. The intervention focused on increasing her knowledge of suffixes in morphologically complex words and teaching her to use this information to improve her vocabulary knowledge, reading, and reading comprehension. Clinically relevant gains were made in the areas of reading and reading comprehension. The results suggest that a literature-based approach in morphological awareness intervention is beneficial for improving the …

Gender Differences In The Knowledge-Base Of Autism Spectrum Disorders, Kayla Williams

Student Theses and Dissertations

Research suggests that the earlier a child with autism spectrum disorder (ASD) is diagnosed, the better their chances for improved functioning. Previous studies have shown the importance of early intervention and parents’ roles in recognizing ASD in their children. Prior studies have not, however, examined which parent recognized the early warning signs. Therefore, the purpose of the current study was to discover which gender had a better understanding of the early indicators of ASD. The primary investigator administered an online true/false and multiple-choice questionnaire regarding the developmental milestones associated with ASD to 110 students at Arkansas State University. The female …

The Parasite Giardia, Ashley Anderson

Honors Program: Senior Projects (Public)

The focus of this research is to better understand how this parasite works. The protozoan Giardia spp is found all over the world and in many species such as dogs, cats, and humans. From its two different forms to the microscopic size of both forms to its ability to destroy intestinal villi and cause symptoms like other parasitic infections, Giardia spp is continuously misdiagnosed. Upon further observation at veterinary clinics and researching science articles, Giardia spp is most commonly found in pets but has the potential to spread to humans via contaminated water. The most commonly infected humans are hikers, …

Association Of Drowning Mortality With Preventive Interventions: A Quarter Of A Million Deaths Evaluation In Brazil, David Szpilman, Danielli B. Mello, Ana Catarina Queiroga, Rogerio Ferreira Emygdio Rfe

International Journal of Aquatic Research and Education

In 2015, drowning in Brazil was responsible for 6,043 deaths and was the second leading cause of death in children. Although several prevention strategies have been promoted to reduce drowning, most are still based on low levels of evidence. This study evaluated the effectiveness of prevention and water safety interventions in reducing drowning mortality. Data obtained from the National Mortality System for 36 years were split in two time periods to allow the comparison of drowning mortality numbers before and after implementation of SOBRASA’s drowning prevention and water safety programs and to check for any positive effects attributable to such …

Health Literacy Related To Modified Barium Swallow Studies, Katherine Johnson

Student Theses and Dissertations

Health literacy is an important factor in client outcomes. Inadequate health literacy can cause individuals to misunderstand health information, seek inappropriate medical treatment, avoid medical treatment, or result in problematic outcomes such as a decrease in health. The purpose of this study was to determine if patients who underwent a modified barium swallow study understood the information (purpose, process, results, and recommendations) regarding the study. There were 15 participants, which included 9 males and 6 females. The Short Assessment of Health Literacy-English (SAHL-E) was used to determine health literacy levels. Out of the 15 participants, 7 males and 5 females …

Student Knowledge Of Preterm Infant Feeding And Swallowing Therapy Protocols, Hailey Ashlyn Hickerson

Student Theses and Dissertations

Given that SLPs have such a wide scope of practice, graduate programs have a large amount of curriculum to cover: language, cognition, articulation, swallowing, pragmatics, and other areas. As a result, graduate students may feel inadequately prepared to work in infant feeding and swallowing settings. If graduate programs adequately educate their students on infant feeding and swallowing, more SLP students may decide to pursue employment and specialize in infant feeding and swallowing intervention to aid in therapy services for vulnerable infants with feeding and swallowing difficulties. The purpose of this study was to examine whether graduate school students feel adequately …

High School Students' Knowledge Of Autism Spectrum Disorder, Kaitlyn Danielle Wiles

Student Theses and Dissertations

There is a major gap and disparity in the age in which children are being diagnosed with autism spectrum disorder (ASD). Research has shown that several factors can contribute to some children being diagnosed at later ages compared to others. Parental age and education are two factors that have been linked to a later diagnosis. The purpose of this study was to identify and examine high school students’ knowledge of the characteristics and diagnosis of ASD. The primary investigator also examined the differences in knowledge between individuals enrolled in rural and urban area high schools. The study revealed some minor …

Exposure, Knowledge, And Experience Of Graduate Students In Communication Disorders On Treating Speech/Language Issues In Adults With Neurological Disorders Using Singing-Based Therapy, Tassie Lee Murphree

Student Theses and Dissertations

There are speech-language pathologists (SLPs) who use singing while in therapy to treat a variety of disorders for adults with neurological disorders. This study was conducted to investigate graduate student exposure to, knowledge of, and experience with utilizing singing in therapy with this population. Specifically, this survey was distributed to first- and second-year graduate students in communication disorders. The survey consisted of both general and specific questions to more effectively analyze the students’ familiarity levels with the topic, overall. The results indicated that the majority of students demonstrated limited knowledge of and exposure to singing in therapy. The students’ comfort …

Collegiate Rugby Players' Knowledge Of Concussions, Julia E. Bell

Student Theses and Dissertations

This investigation sought to discover if collegiate rugby players who have had a concussion were more knowledgeable about concussions than the players who had not had a concussion. The investigation also looked at the general amount of knowledge that the collegiate rugby players had about concussions, including the effects and prevention measures. This study was performed using a survey distributed to the Arkansas State University club rugby team. The survey consisted of several questions about concussions, the side effects of concussions, and players’ opinions on prevention and education of concussions. The data collection revealed that there were differing amounts of …

Modified Glucose-Insulin-Potassium Regimen Provides Cardioprotection With Improved Tissue Perfusion In Patients Undergoing Cardiopulmonary Bypass Surgery, Kun Zhao, Yue Zhang, Jia Li, Qin Cui, Rong Zhao, Wensheng Chen, Jincheng Liu, Bijun Zhao, Yi Wan, Xin-Liang Ma, Shiqiang Yu, Dinghua Yi, Feng Gao

Department of Emergency Medicine Faculty Papers

Background Laboratory studies demonstrate glucose-insulin-potassium (GIK) as a potent cardioprotective intervention, but clinical trials have yielded mixed results, likely because of varying formulas and timing of GIK treatment and different clinical settings. This study sought to evaluate the effects of modified GIK regimen given perioperatively with an insulin-glucose ratio of 1:3 in patients undergoing cardiopulmonary bypass surgery. Methods and Results In this prospective, randomized, double-blinded trial with 930 patients referred for cardiac surgery with cardiopulmonary bypass, GIK (200 g/L glucose, 66.7 U/L insulin, and 80 mmol/L KCl) or placebo treatment was administered intravenously at 1 mL/kg per hour 10 minutes …

The Gender Gap In Alcohol Deaths Is Much Larger In Some States Than Others, Erin Bisesti

Population Health Research Brief Series

Alcohol-related deaths have been on the rise in the U.S. over the past several years. Men have higher rates of alcohol-related death than women, and the gender gap is largest in the western and southern regions of the U.S. This brief describes which states have the highest rates of alcohol-related death among men and women.

2020-03-17 A Message From Chancellor Roth, Paul B. Roth

HSC Messages from the Chancellor

No abstract provided.

Proteins Inform Survival-Based Differences In Patients With Glioblastoma, L. C. Stetson, Daniela M. Schlatzer, Peter Liao, Karen Devine, Kristin Waite, Marta E. Couce, Andrew E. Sloan, Mark R. Chance, Jill S. Barnholtz-Sloan

Faculty Scholarship

Background: Improving the care of patients with glioblastoma (GB) requires accurate and reliable predictors of patient prognosis. Unfortunately, while protein markers are an effective readout of cellular function, proteomics has been underutilized in GB prognostic marker discovery. Methods: For this study, GB patients were prospectively recruited and proteomics discovery using liquid chromatography-mass spectrometry analysis (LC-MS/MS) was performed for 27 patients including 13 short-term survivors (STS) (≤10 months) and 14 long-term survivors (LTS) (≥18 months). Results: Proteomics discovery identified 11 941 peptides in 2495 unique proteins, with 469 proteins exhibiting significant dysregulation when comparing STS to LTS. We verified the differential …

Abrogation Of Esophageal Carcinoma Development In Mir-31 Knockout Rats., Louise Y Fong, Cristian Taccioli, Alexey Palamarchuk, Guidantonio Malagoli Tagliazucchi, Ruiyan Jing, Karl J Smalley, Sili Fan, Joseph Altemus, Oliver Fiehn, Kay Huebner, John L Farber, Carlo M Croce

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

MicroRNA-31 (miR-31) is overexpressed in esophageal squamous cell carcinoma (ESCC), a deadly disease associated with dietary Zn deficiency and inflammation. In a Zn deficiency-promoted rat ESCC model with miR-31 up-regulation, cancer-associated inflammation, and a high ESCC burden following N-nitrosomethylbenzylamine (NMBA) exposure, systemic antimiR-31 delivery reduced ESCC incidence from 85 to 45% (P = 0.038) and miR-31 gene knockout abrogated development of ESCC (P = 1 × 10-6). Transcriptomics, genome sequencing, and metabolomics analyses in these Zn-deficient rats revealed the molecular basis of ESCC abrogation by miR-31 knockout. Our identification of EGLN3, a known negative regulator of nuclear …

Unrevealing Sequence And Structural Features Of Novel Coronavirus Using In Silico Approaches: The Main Protease As Molecular Target, Joseph Thomas Ortega

Faculty Scholarship

Direct-acting antivirals are effective tools to control viral infections. SARS-CoV-2 is a coronavirus associated with the epidemiological outbreak in late 2019. Previous reports showed that HIV-1 protease inhibitors could block SARS-CoV main protease. Based on that and using an in silico approach, we evaluated SARS-CoV-2 main protease as a target for HIV-1 protease inhibitors to reveal the structural features related to their antiviral effect. Our results showed that several HIV inhibitors such as lopinavir, ritonavir, and saquinavir produce strong interaction with the active site of SARS-CoV-2 main protease. Furthermore, broad library protease inhibitors obtained from PubChem and ZINC (www.zinc.docking.org) were …

Graduate Occupational Therapy Students & Information Literacy: Does A Research Consultation Increase Information Literacy Skills While Lowering Library Anxiety?, Mary C. Rickelman, Christine Moghimi

Transforming Libraries for Graduate Students

In today’s academic environment, students equipped with self-regulated learning and information literacy skills have an excellent opportunity for professional success, given our current information-based practices in health care. Information literacy instruction provided to students early in their coursework will aide them in acquiring competency for the remainder of their studies, especially scholarly projects. Along with information literacy issues, library anxiety has been identified in the literature as a contributing factor to poor academic performance in students. The purpose of this study was to explore components of library anxiety, if present, as well as to help students become more comfortable and …

Acoustic Characteristics Of Word-Final American English Liquids Produced By L2 Adult Speakers, Judith A. Espinal

LSU Master's Theses

In this study, the acoustic differences between native English speakers’ (L1) and native-Korean speakers’ (L2) production of American English liquids /ɹ/, /l/ and /ɹl/ were examined among 14 Korean speakers and 13 English speakers. Temporal measures included (1) relative timing of maximum constriction and (2) duration of vocalic nuclei. Spectral measures included (1) Euclidean distance between /ɹ/ and /l/ and (2) frequency difference between F2 and F3. The results indicated a significant interaction between speaker group and phonetic stimuli. That is, L2 speakers produced a similar degree of constriction across semivowels, whereas L1 speakers produced varying degrees of F2-F3 constrictions …

Students As Investigators: Utilizing Active Learning To Engage Genetic Counseling Students, Stacey E. Wahl Ph.D., Dana L. Ladd Ph.D.

Transforming Libraries for Graduate Students

Providing one-shot instruction sessions can be difficult, particularly in the graduate learning environment. As librarians, we want to provide students with the skills to search effectively for health information without overwhelming or confusing them. In health science graduate programs, we are expected to connect literature searching skills with the content of the courses in a manner that is engaging to students. This challenge can be exacerbated when students are new to graduate school and have not yet become familiar with scientific literature searching or the research process. Two medical librarians sought to overcome these challenges by empowering genetic counseling students …

Librarian At The Colloquium: Delivering Unique Library Content For Phd Students, Susan Franzen

Transforming Libraries for Graduate Students

PhD students have unique needs and require different resources and services from the library than undergraduates, which is especially true of professionals in a nursing program. As clinicians, many do not have experience with the research and writing intensive requirements of a doctoral degree. The majority have not taken classes for years, and their master’s degrees were more hands-on, clinically-based. They often do not feel confident in their ability to search the literature, read closely, or write expansively. A unique avenue through which to meet their needs and share library resources is a PhD colloquium course.

Students take the colloquium …

Trends In The Sources Of Endophthalmitis At Kresge Eye Institute, Anita Vaishampayan, Kresge Eye Institute

Medical Student Research Symposium

Purpose

Endophthalmitis is a defined as a purulent inflammation of the intraocular fluids, namely the vitreous and aqueous humor. Endophthalmitis most commonly develops from infection, but can also be acquired from inflammatory and neoplastic conditions. We sought to determine these sources at our institute and to look for trends in our urban population.

Methods

A retrospective chart review of patients who attended the Kresge Eye Institute in Detroit, MI between 2003 to 2018 was performed. Patient list was identified from our EMR using the diagnoses codes related endophthalmitis. Information gathered included demographics, type of tissue/fluid cultured, the presumed source of …

Management Of Childhood Migraine By Headache Specialist Versus Non-Headache Specialists, Kelly Valentini, Radhika Gutta, Gunjanpreet Kaur, Ahmad Farooqi, Lalitha Sivaswamy

Medical Student Research Symposium

This study aims to compare the management practices of a headache specialist with non-headache specialists in the treatment of pediatric migraine. The use of appropriate rescue medications and prophylactic agents, application of neuro-imaging, and short-term outcomes are compared in children treated by the two groups of physicians. A retrospective cohort study was conducted utilizing the electronic medical records of children 3-18 years of age with migraine, who were evaluated at a tertiary care children’s hospital from 2016-2018. Of the 849 patients that met the study criteria, 469 were classified as having chronic migraine or high-frequency episodic migraine and were followed …