Medicine and Health Sciences Commons^™

Longitudinal Analysis Of Change In Mammographic Density In Each Breast And Its Association With Breast Cancer Risk, Shu Jiang, Debbie L Bennett, Bernard A Rosner, Graham A Colditz

2020-Current year OA Pubs

IMPORTANCE: Although breast density is an established risk factor for breast cancer, longitudinal changes in breast density have not been extensively studied to determine whether this factor is associated with breast cancer risk.

OBJECTIVE: To prospectively evaluate the association between change in mammographic density in each breast over time and risk of subsequent breast cancer.

DESIGN, SETTING, AND PARTICIPANTS: This nested case-control cohort study was sampled from the Joanne Knight Breast Health Cohort of 10 481 women free from cancer at entry and observed from November 3, 2008, to October 31, 2020, with routine screening mammograms every 1 to 2 …

Acinetobacter Baumannii Atcc 17978 Encodes A Microcin System With Antimicrobial Properties For Contact-Independent Competition, Fabiana Bisaro, Howard A Shuman, Mario F Feldman, Michael J Gebhardt, Stefan Pukatzki

2020-Current year OA Pubs

No abstract provided.

Crypticproteindb: An Integrated Database Of Proteome And Immunopeptidome Derived Non-Canonical Cancer Proteins, Ghofran Othoum, Christopher A Maher

2020-Current year OA Pubs

Translated non-canonical proteins derived from noncoding regions or alternative open reading frames (ORFs) can contribute to critical and diverse cellular processes. In the context of cancer, they also represent an under-appreciated source of targets for cancer immunotherapy through their tumor-enriched expression or by harboring somatic mutations that produce neoantigens. Here, we introduce the largest integration and proteogenomic analysis of novel peptides to assess the prevalence of non-canonical ORFs (ncORFs) in more than 900 patient proteomes and 26 immunopeptidome datasets across 14 cancer types. The integrative proteogenomic analysis of whole-cell proteomes and immunopeptidomes revealed peptide support for a nonredundant set of …

Peptidoglycan Recognition In Drosophila Is Mediated By Lysmd3/4, Mark Snee, Jason Wever, Jennifer Guyton, Ryan Beehler-Evans, Christine C Yokoyama, Craig A Micchelli

2020-Current year OA Pubs

Microbial recognition is a key step in regulating the immune signaling pathways of multicellular organisms. Peptidoglycan, a component of the bacterial cell wall, exhibits immune stimulating activity in both plants and animals. Lysin motif domain (LysMD) family proteins are ancient peptidoglycan receptors that function in bacteriophage and plants. This report focuses on defining the role of LysMD-containing proteins in animals. Here, we characterize a novel transmembrane LysMD family protein. Loss-of-function mutations at the lysMD3/4 locus in Drosophila are associated with systemic innate immune activation following challenge, so we refer to this gene as immune active (ima). We show that Ima …

Evaluating Motion Of Pancreatic Tumors And Anatomical Surrogates Using Cine Mri In 0.35t Mrgrt Under Free Breathing Conditions, Benjamin Lewis, Anamaria Guta, Jaeik Shin, Zhen Ji, Jin Sung Kim, Taeho Kim

2020-Current year OA Pubs

Treatment tolerability is a significant limitation to pancreatic cancer treatment with radiotherapy due to proximity to highly radiosensitive organs and respiratory motion necessitating expanded target margins. Further, pancreatic tumors are difficult to visualize on conventional radiotherapy systems. Surrogates are often used to locate the tumor but are often inconsistent and do not provide strong positional relations throughout the respiratory cycle. This work utilizes a retrospective dataset of 45 pancreatic cancer patients treated on an MR-Linac system with cine MRI acquired for real-time target tracking. We investigated intra-fraction motion of tumors and two abdominal surrogates, leading to prediction models between the …

Temperament & Character Account For Brain Functional Connectivity At Rest: A Diathesis-Stress Model Of Functional Dysregulation In Psychosis, Igor Zwir, Javier Arnedo, Alberto Mesa, Coral Del Val, Gabriel A De Erausquin, C Robert Cloninger

2020-Current year OA Pubs

The human brain's resting-state functional connectivity (rsFC) provides stable trait-like measures of differences in the perceptual, cognitive, emotional, and social functioning of individuals. The rsFC of the prefrontal cortex is hypothesized to mediate a person's rational self-government, as is also measured by personality, so we tested whether its connectivity networks account for vulnerability to psychosis and related personality configurations. Young adults were recruited as outpatients or controls from the same communities around psychiatric clinics. Healthy controls (n = 30) and clinically stable outpatients with bipolar disorder (n = 35) or schizophrenia (n = 27) were diagnosed by structured interviews, and …

Ex Vivo Nonbiomaterial Gel-Based Model For Endoscopic Training, Ameya Deshmukh, Juan Reyes Genere, Ahmad Najdat Bazarbashi

2020-Current year OA Pubs

Video 1Use of artificial model for endoscopic training.

Defects In Lysosomal Function And Lipid Metabolism In Human Microglia Harboring A Trem2 Loss Of Function Mutation, Fabia Filipello, Shih-Feng You, Farzaneh S Mirfakhar, Sidhartha Mahali, Bryan Bollman, Mariana Acquarone, Jacob A Marsh, Anirudh Sivaraman, Rita Martinez, Claudia Cantoni, Luca De Feo, Laura Ghezzi, Miguel A Minaya, Arun Renganathan, Anil G Cashikar, Wandy Beatty, Abhirami K Iyer, Marina Cella, Laura Piccio, Celeste M Karch, Et Al.

2020-Current year OA Pubs

TREM2 is an innate immune receptor expressed by microglia in the adult brain. Genetic variation in the TREM2 gene has been implicated in risk for Alzheimer's disease and frontotemporal dementia, while homozygous TREM2 mutations cause a rare leukodystrophy, Nasu-Hakola disease (NHD). Despite extensive investigation, the role of TREM2 in NHD pathogenesis remains poorly understood. Here, we investigate the mechanisms by which a homozygous stop-gain TREM2 mutation (p.Q33X) contributes to NHD. Induced pluripotent stem cell (iPSC)-derived microglia (iMGLs) were generated from two NHD families: three homozygous TREM2 p.Q33X mutation carriers (termed NHD), two heterozygous mutation carriers, one related non-carrier, and two …

Toward Blood-Based Precision Medicine: Identifying Age-Sex-Specific Vascular Biomarker Quantities On Circulating Vascular Cells, Yingye Fang, Ling Chen, P I Imoukhuede

2020-Current year OA Pubs

INTRODUCTION: Abnormal angiogenesis is central to vascular disease and cancer, and noninvasive biomarkers of vascular origin are needed to evaluate patients and therapies. Vascular endothelial growth factor receptors (VEGFRs) are often dysregulated in these diseases, making them promising biomarkers, but the need for an invasive biopsy has limited biomarker research on VEGFRs. Here, we pioneer a blood biopsy approach to quantify VEGFR plasma membrane localization on two circulating vascular proxies: circulating endothelial cells (cECs) and circulating progenitor cells (cPCs).

METHODS: Using quantitative flow cytometry, we examined VEGFR expression on cECs and cPCs in four age-sex groups: peri/premenopausal females (aged < 50 years), menopausal/postmenopausal females (≥ 50 years), and younger and older males with the same age cut-off (50 years).

RESULTS: …

Replicative Acinetobacter Baumannii Strains Interfere With Phagosomal Maturation By Modulating The Vacuolar Ph, Jesus S. Distel, Gisela Di Venanzio, Joseph J. Mackel, David A. Rosen, Mario F. Feldman

2020-Current year OA Pubs

Bacterial pneumonia is a common infection of the lower respiratory tract that can afflict patients of all ages. Multidrug-resistant strains of Acinetobacter baumannii are increasingly responsible for causing nosocomial pneumonias, thus posing an urgent threat. Alveolar macrophages play a critical role in overcoming respiratory infections caused by this pathogen. Recently, we and others have shown that new clinical isolates of A. baumannii, but not the common lab strain ATCC 19606 (19606), can persist and replicate in macrophages within spacious vacuoles that we called Acinetobacter Containing Vacuoles (ACV). In this work, we demonstrate that the modern A. baumannii clinical isolate 398, …

Finding Motifs Using Dna Images Derived From Sparse Representations, Shane K Chu, Gary D Stormo

2020-Current year OA Pubs

MOTIVATION: Motifs play a crucial role in computational biology, as they provide valuable information about the binding specificity of proteins. However, conventional motif discovery methods typically rely on simple combinatoric or probabilistic approaches, which can be biased by heuristics such as substring-masking for multiple motif discovery. In recent years, deep neural networks have become increasingly popular for motif discovery, as they are capable of capturing complex patterns in data. Nonetheless, inferring motifs from neural networks remains a challenging problem, both from a modeling and computational standpoint, despite the success of these networks in supervised learning tasks.

RESULTS: We present a …

Participant- And Disease-Related Factors As Independent Predictors Of Treatment Outcomes In The Restore-Imi 2 Clinical Trial: A Multivariable Regression Analysis, Ignacio Martin-Loeches, Andrew F Shorr, Marin H Kollef, Jiejun Du, Maria C Losada, Amanda Paschke, C Andrew Deryke, Michael Wong, Erin H Jensen, Luke F Chen

2020-Current year OA Pubs

BACKGROUND: In the RESTORE-IMI 2 trial, imipenem/cilastatin/relebactam (IMI/REL) was noninferior to piperacillin/tazobactam in treating hospital-acquired bacterial pneumonia/ventilator-associated bacterial pneumonia. This post hoc analysis was conducted to determine independent predictors of efficacy outcomes in the RESTORE-IMI 2 trial, to assist in treatment decision making.

METHODS: A stepwise multivariable regression analysis was conducted to identify variables that were independently associated with day 28 all-cause mortality (ACM), favorable clinical response at early follow-up (EFU), and favorable microbiologic response at end of treatment (EOT). The analysis accounted for the number of baseline infecting pathogens and in vitro susceptibility to randomized treatment.

RESULTS: Vasopressor use, …

Assessment Of Brain Magnetic Resonance And Spectroscopy Imaging Findings And Outcomes After Pediatric Cardiac Arrest, Ericka L Fink, Stuart Friess, Et Al.

2020-Current year OA Pubs

IMPORTANCE: Morbidity and mortality after pediatric cardiac arrest are chiefly due to hypoxic-ischemic brain injury. Brain features seen on magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) after arrest may identify injury and aid in outcome assessments.

OBJECTIVE: To analyze the association of brain lesions seen on T2-weighted MRI and diffusion-weighted imaging and N-acetylaspartate (NAA) and lactate concentrations seen on MRS with 1-year outcomes after pediatric cardiac arrest.

DESIGN, SETTING, AND PARTICIPANTS: This multicenter cohort study took place in pediatric intensive care units at 14 US hospitals between May 16, 2017, and August 19, 2020. Children aged 48 hours …

Safety And Efficacy Of Vanzacaftor-Tezacaftor-Deutivacaftor In Adults With Cystic Fibrosis: Randomised, Double-Blind, Controlled, Phase 2 Trials, Ahmet Z Uluer, Ronald C Rubenstein, Et Al.

2020-Current year OA Pubs

BACKGROUND: Elexacaftor-tezacaftor-ivacaftor has been shown to be safe and efficacious in people with cystic fibrosis and at least one F508del allele. Our aim was to identify a novel cystic fibrosis transmembrane conductance regulator (CFTR) modulator combination capable of further increasing CFTR-mediated chloride transport, with the potential for once-daily dosing.

METHODS: We conducted two phase 2 clinical trials to assess the safety and efficacy of a once-daily combination of vanzacaftor-tezacaftor-deutivacaftor in participants with cystic fibrosis who were aged 18 years or older. A phase 2 randomised, double-blind, active-controlled study (VX18-561-101; April 17, 2019, to Aug 20, 2020) was carried out to …

A Functional Genomics Pipeline To Identify High-Value Asthma And Allergy Cpgs In The Human Methylome, Andréanne Morin, Katherine Rivera-Spoljaric, Et Al.

2020-Current year OA Pubs

BACKGROUND: DNA methylation of cytosines at cytosine-phosphate-guanine (CpG) dinucleotides (CpGs) is a widespread epigenetic mark, but genome-wide variation has been relatively unexplored due to the limited representation of variable CpGs on commercial high-throughput arrays.

OBJECTIVES: To explore this hidden portion of the epigenome, this study combined whole-genome bisulfite sequencing with in silico evidence of gene regulatory regions to design a custom array of high-value CpGs. This study focused on airway epithelial cells from children with and without allergic asthma because these cells mediate the effects of inhaled microbes, pollution, and allergens on asthma and allergic disease risk.

METHODS: This study …

Early Experiences Of Parents Of Children Who Are Deaf Or Hard Of Hearing: Navigating Through Identification, Intervention, And Beyond, Gabriella Reynolds, Krystal L. Werfel, Morgan Vachio, Emily A. Lund

Journal of Early Hearing Detection and Intervention

Guidelines created by the Joint Committee on Infant Hearing ([JCIH], 2019) were designed to aid in the early identification of infant hearing loss. Despite these guidelines, a quarter of children who fail their initial screening are lost to the follow-up process and many more do not receive care in line with the 1-3-6 guidelines (Centers for Disease Control and Prevention [CDC], 2018; JCIH, 2019). To acquire more information about the experiences of families and identify specific barriers to timely diagnosis and intervention, interviews were conducted with 13 parents of children who are deaf or hard of hearing whose children were …

The Signed Linguistic Input Of Hearing Mothers To Children Who Are Deaf Or Hard Of Hearing, Aleah S. Brock

Journal of Early Hearing Detection and Intervention

This study investigated the distribution of ten facilitative language techniques (FLTs) in the linguistic input of hearing, signing mothers to their children who are deaf or hard of hearing (DHH). Four hearing mothers and their DHH children under the age of three participated in six, ten-minute caregiver-child interaction sessions via Zoom. The recorded sessions were coded for mother FLTs and child utterances. Results indicated that the mothers tended to use more initiative than responsive types of FLTs, consistent with findings of previous studies that examined the input of mothers who were using spoken-only language with their children who are DHH. …

Improving South Dakota Parents’ Knowledge Of Congenital Cytomegalovirus, Hannah M. Williams, Jessica Messersmith, Jacynda Gellhaus

Journal of Early Hearing Detection and Intervention

Introduction: Congenital cytomegalovirus (cCMV) is the most common infectious condition present at birth and the leading non-genetic cause of sensorineural hearing loss in children. Despite CMV being common and preventable, knowledge of CMV remains low among individuals in the United States (Doutre et al., 2016). Sudden infant death syndrome (SIDS) is also common among infants in the United States, but unlike cCMV, several studies have researched which educational methods have been most effective for improving parents’ knowledge of safe sleep practices. The purpose of this study was to investigate whether educational methods shown to be most effective for improving parents’ …

Adverse Childhood Experiences In Mothers And Their Children With Hearing Loss, Elizabeth A. Rosenzweig

Journal of Early Hearing Detection and Intervention

This study examined the relationship between maternal and child ACEs in children with hearing loss ages 3-12. One hundred twenty-four mother-child dyads completed assessments of mother and child ACEs. Adverse cChildhood eExperiences (ACEs) were measured using the Center for Youth Wellness Adverse Childhood Experiences Questionnaire (CYW ACE-Q; Burke Harris & Renschler, 2015).

Both maternal and child participants in this study reported higher levels of ACE exposure than previously reported in studies of the general population. Maternal and child ACEs were significantly correlated. White/Caucasian mothers experienced significantly fewer ACEs than mothers of other races/ethnicities. Children living in adoptive, foster, or guardianship …

Implementing A Hearing Screening Readiness Assessment Tool For Preterm And Term Neonates In The Newborn Intensive Care Unit: A Pilot Project, Mckenzie Blatt, Adrienne Johnson, Jennifer L. Hamilton, Carly Alston, Belinda Chan

Journal of Early Hearing Detection and Intervention

Newborn intensive care unit (NICU) patients are at risk for hearing loss. Early detection mitigates consequences of speech and language delay. The Joint Committee on Infant Hearing (JCIH) recommends hearing screening (HS) on all infants by 1 month of age. Often hearing screening is performed around the time of NICU discharge, often beyond JCIH recommendations. Automated auditory brainstem response (AABR) screening can be performed once an infant reaches 32 to 34 weeks corrected gestational age (GA). Our project aimed to reduce HS delay among NICU infants. We created and implemented a HS assessment tool defining gestational age and medical stability …

Highlights Of The 2022 Brano Heart Failure Forum: Part One, Ugo Livi, Rajko Radovancevic, Bojan Vrtovec, Igor D. Gregoric

The VAD Journal

Since 2007, the Branislav “Brano” Radovancevic Heart Failure Forum (BHFF) has been held annually to provide a venue for experts to present and discuss “Innovations and New Treatment Strategies in Heart Failure.” Clinicians and researchers gather yearly in a different Eastern European city to discuss the latest in heart failure diagnostics and therapeutics. The 2022 BHHF forum was held on the 6^th thru 8^th of September 2022 in Trieste, Italy. It was attended by over 94 faculty from 14 countries. In addition, participation through online streaming was available. Throughout the forum, 17 sessions focused on challenges and solutions …

Effectiveness Of Couples-Based Exposure And Response Prevention (Erp) Therapy Vs. Individual Erp Treatment For Ocd Symptom Reduction And Overall Couple Satisfaction, Priscilla Nguyen, Shana O'Connor-Wolf

School of Psychology, Family, and Community Research Conference

Obsessive-Compulsive Disorder (OCD) is a debilitating, chronic condition characterized by recurrent intrusive thoughts followed by persistent behavioral urges and time-consuming rituals that attempt to neutralize anxiety and distress caused by those unwanted obsessive thoughts (American Psychiatric Association, 2013). This research study aims to explore the effectiveness of Couples-Based ERP in decreasing individual symptoms of OCD and evaluating the impact of treatment on relationship satisfaction. Using the Yale-Brown Obsessive Compulsive Scale (Y-BOCS) and the Dyadic-Adjustment Scale (DAS) to measure our constructs, we hypothesize that Couples-Based ERP will be effective in not only decreasing OCD symptoms, but also increasing overall relationship satisfaction.

A Systematic Review Of Bottom-Up And Top-Down Psychotherapy Methods For The Treatment Of Posttraumatic Stress Disorder (Ptsd), Kristen Sievert

School of Psychology, Family, and Community Research Conference

While numerous studies have investigated the efficacy of either Top-Down or Bottom-Up modalities in reducing PTSD symptoms, there is a paucity of research focused on comparing differences in treatment effects of Bottom-Up and Top-Down therapies. Top-Down interventions are built on an individual’s ability to become conscious of their thoughts and their subsequent capacity to change those thoughts, while Bottom-Up interventions rely on an individual’s body sensations an/or movements to access and process trauma. We investigated this gap by conducting a systematic review of Top-down and Bottom-up treatments for PTSD. To be included in the review, studies needed to examine adults …

The Effectiveness Of Combining Play Therapy With Emotionally Focused Family Therapy For The Treatment Of Children, Ages 3-6., Britney Roark

School of Psychology, Family, and Community Research Conference

Present literature on emotionally focused family therapy (EFFT) overlooks treatment involving young children (Willis et al., 2016). Because preschool-aged children learn and express emotion best through the medium of play (Schaefer & Drewes, 2011), I hypothesize that EFFT will prove most effective with children ages 3-6 when combined with play therapy techniques. Marriage and family therapy interns, trained in EFFT concepts, who treat children in this age group will complete a training course on combining play therapy with EFFT. A control group will not. Initial and final measures will determine the success of treatment over a 12-week period.

Efficacy Of Solution Focused Therapy With At Risk Youth, Garret Patterson

School of Psychology, Family, and Community Research Conference

Dating back to the 1980’s, Solution Focused Brief Therapy (SFBT) is a therapy approach that helps clients put problems in their life into context as measurable, manageable, and fixable. For many, this solution forward approach helps to improve symptoms and resolve stuck behavior patterns often stemming from childhood. This client driven, not knowing approach helps clients discover their own autonomy and understand that not only are their problems solvable, but that they themselves have the internal resources by which to solve them. However, there is still much interest as to who can benefit most from this form of therapy. However, …

Engagement In Religious Activity Compared To Peer Support: An Intervention For Suicidal Ideation Among High School Students., Meradythe Edson, Natalie Goobes, Danielle Thayer

School of Psychology, Family, and Community Research Conference

Suicidal ideation (SI) can lead to suicide. Suicide is the second leading cause of death among children ages 10-14 and the third leading cause of death among adolescents ages 15-24 (CDC). This study compares active engagement in religious activities versus peer support as an effective resource in decreasing SI among high school students in the United States. The research explains how active engagement in religious activities will produce better results than peer support alone for decreasing SI in the targeted population. Semi-structured interviews of high school students and self-report questionnaires are utilized in this study. Our research hopes to support …

The Effectiveness Of Video Game Therapy In Helping Adolescent Clients To Build Connections With Their Families, Qichang Yang

School of Psychology, Family, and Community Research Conference

This study explores the role of video game therapy in enhancing family connections among adolescents. Using a mixed-methods approach, we use surveys and interviews to assess changes in family relationships for adolescents undergoing video game therapy. Preliminary findings indicate that video game therapy could be a great tool for improving familial bonds. This research adds to the understanding of non-traditional therapeutic interventions and their potential impact on family dynamics and adolescent mental health.

Impacts Of Receiving An Autism Spectrum Diagnosis As An Adult On Relationship Satisfaction And Relationship-Specific Attachment, Erika Fisch, Molly Hammond, Katie Roberts

School of Psychology, Family, and Community Research Conference

Our research will explore how receiving an Autism Spectrum Disorder diagnosis impacts adult relationship satisfaction and relationship-specific attachment. Previous research has demonstrated benefits of ASD diagnosis for individuals. We hope to expand this body of research by examining the effects of ASD diagnosis on couples. We will survey adult couples with one partner on a waitlist for an ASD evaluation using the Dyadic Adjustment Scale and the Experiences in Close Relationships-Revised questionnaire. After evaluation, we will ask adults diagnosed with ASD and their partners to take the survey twice more. We hypothesize that both relationship satisfaction and attachment will improve.

Effectiveness Of Emotionally Focused Family Therapy (Efft) On Adolescent Non-Suicidal Self-Injury (Nssi) Vs Dialectical Behavioral Therapy For Adolescence (Dbt-A), Aneta Merlino

School of Psychology, Family, and Community Research Conference

This study aims to explore the effectiveness of Emotionally Focused Family Therapy (EFFT) in reducing the incidence of adolescent Non-Suicidal Self Injury (NSSI). 80 adolescents who meet the NSSI criteria, age 13-19, will be randomized to participate in a total of 12 weekly, in-person EFFT sessions, or 16 weekly in-person Dialectical Behavioral Therapy for Adolescence (DBT-A) sessions. It is hypothesized EFFT will be more effective in long term reduction of NSSI frequency, measured post treatment, and at 12 month follow up, due to an increase in interpersonal protective factors as compared to DBT-A.

Are Telehealth Interventions Efficacious In Reducing Symptoms Of Depression And Anxiety?, Megan Hoback, Mario Gaspar De Alba

School of Psychology, Family, and Community Research Conference

This study aims to explore the efficacy of therapeutic interventions via telehealth on reducing depression and anxiety symptoms. The focus will be on individuals living in the United States diagnosed with Major Depressive Disorder (MDD) and/or Generalized Anxiety Disorder (GAD). Subjects will participate in 12 individual telehealth sessions conducted across three months with a pre-test, immediate post-test, and 3-month follow up including completion of the Patient Health Questionnaire (PHQ-9), Generalized Anxiety Disorder Screener (GAD-7), and the Telehealth Satisfaction Questionnaire (TSQ) to measure efficacy. We hypothesize therapeutic interventions via telehealth will be effective in reducing symptoms of depression and anxiety.