Medicine and Health Sciences Commons^™

A Translational Approach Towards More Cost-Effective Lung Cancer Treatments, Tuula Klaavuniemi, Subhash C. Chauhan, Ville Paappanen, Artturi Mäkinen, Tuomas Rauramaa, Juha Väyrynen, Arja Jukkola, Hanne Kuitunen, Outi Kuittinen

Research Symposium

Lung cancer, globally the second most common cancer, causes 1.8 million deaths annually with 2 million new cases. It's also one of Finland's deadliest cancers. Chemotherapeutic agents, targeted drugs and radiation therapy have been the mainstay in oncologic treatment for lung cancer patients for decades. Checkpoint inhibitors (e.g. nivolumab, pembrolizumab, atezolizumab) have changed the landscape also in lung cancer. These drugs awake patients own immune system to attack cancer cells. These drugs are widely used in different cancers and they have efficacy also in lung cancer, but only in a minority of patients. Checkpoint inhibitors also induce autoimmune side effects. …

A Novel Exo-Glow Nano-System For Bioimaging, Nycol Cotto, Benilde Adriano, Neeraj Chauhan, Deepak S. Chauhan, Meena Jaggi, Subhash C. Chauhan, Murali M. Yallapu

Research Symposium

Background: Milk exosomes are widely used to improve the performance of various small macromolecules, oligonucleotides, and imaging agents for delivery and imaging applications. Indocyanine green (ICG) based Near-Infrared (NIR) fluorescent imaging is an attractive and safer technique used for number of clinical applications. However, ICG tend to have poor photostability, short half-life, non-specific proteins binding, and concentration-dependent aggregation. Therefore, there is an unmet clinical need to develop newer modalities to package and deliver ICG. Bovine milk exosomes are natural, biocompatible, safe, and feasible nanocarriers that facilitate the delivery of micro and macro molecules. Herein, we developed a novel exosomes based …

Near-Neutral Balanced Selection Theory (Nnbst): Implication On The Molecular Evolution Of Sars-Cov-2 And Zika Virus, Nicholas J. Paradis

Graduate Student and Postdoctoral Fellow Symposium

Virus pandemics have significantly impacted human welfare and development. Understanding the true molecular evolution of viruses is critical for preventing future pandemics and developing the next-generation drugs and vaccines. Unfortunately, viruses exhibiting constant genomic substitution rates (GSRs) despite increasing vaccinations and human infections (SARS-CoV-2) and viruses under seemingly extreme conservation (ZIKV) paints a confusing picture of viral evolution, and the main evolutionary theories (Selectionist Theory, Kimura’s Neutral Theory) cannot explain their molecular evolution. Here, we developed a first-principle model, c/u, to characterize reproductive fitness changes in viruses based on variations in the nucleotide/codon mutation rate (c) with respect to the …

The Imitation Game: Drug Reaction Presenting As Autoimmunity Presenting As Syphilis, Victoria W. Murray, Liam Courtney, Judith Lightfoot

Graduate Student and Postdoctoral Fellow Symposium

Introduction: Syphilis screening, a common medical test in the US, is recommended annually in sexually active women younger than 25, annually for women older than 25, and annually for men(1). Standard tests include rapid reagin tests and Venereal Disease Research Laboratory tests, which react to membrane antigens of Treponema pallidum(2). Case reports of patients with autoimmune conditions have been described (3) but rarely considered in clinical practice. Additionally, patients may be underdiagnosed with autoimmune conditions with clinically relevant antibodies (4). Here, we describe one such case where a patient received delay in care prior to referral to our service. Case …

Repetitive Negative Thinking As A Prospective Predictor Of Depression And Anxiety Symptoms In Autistic And Non-Autistic Incoming College Students, Erin Kckenney

Graduate Student and Postdoctoral Fellow Symposium

Background: Improving the understanding and treatment of mental health concerns, including depression and anxiety, are significant priorities for autistic adults (Benevides et al., 2020; Crane et al., 2019; Pellicano et al., 2014). While several theories have been proposed to explain the high prevalence of internalizing symptoms in autistic people, little longitudinal research has been done to investigate potential causal mechanisms. One potentially fruitful area of investigation is to explore how well-known contributors to depression/anxiety in the general population predict and/or moderate the development of internalizing symptoms in autistic individuals. Objectives: The current study investigates the relation of one established risk …

Black Boys Om: A Case Study Of The Impacts Of Community And Culture On Wellness Practices, Khanh Pham

Graduate Student and Postdoctoral Fellow Symposium

Black men continue to face health disparities due to systemic oppression. Research indicates that mind-body-spirit practices (MBSPs) can effectively improve mental and physical health, thereby reducing health disparities among Black men. However, MBSPs are often White-female-dominated practices, thus putting Black male practitioners at continued risks of under-representation and marginalization in MBSP spaces. The culturally specific nature of MBSP spaces may then be an important factor in MBSPs’ influence on Black men’s health. Therefore, we aimed to explore how culturally specific MBSP spaces impact Black male practitioners’ MBSPs. We collaborated with Black Boys OM (BBO), a global community of Black male …

The Impact Of Covid-19 On The Utilization Of Early Magnetic Resonance Imaging (Emri) In The Assessment Of Acute Uncomplicated Low Back Pain (Lbp) And The Subsequent Effect On Health Care Service Utilization And Patient Outcomes, Kimberly F. Radcliffe

MUSC Theses and Dissertations

OBJECTIVES: To examine changes in MRI rates between Pre-COVID-19 period (Feb-April 2019) and COVID-19 period (Feb-April 2020) among commercially insured patients aged 18-60 with a diagnosis of acute LBP, and to analyze differences in patient characteristics and outcomes between the time periods. Additionally, to examine the impact of eMRI on patient outcomes and health care costs for patients in the COVID-19 period.

DESIGN/METHODS: Using IBM® MarketScan® Commercial Database (MarketScan) we performed a quantitative pre-post comparative retrospective observational study of 117,150 total patients to examine adjusted differences in patient characteristics and rates of MRIs between time periods. We analyzed 49,020 patients …

Readmission Rates Of Mothers Experiencing Preeclampsia Or Complications After Delivery In Underserved Populations, Sadia M. Robinson

MUSC Theses and Dissertations

Hospital readmissions during pregnancy nationwide impacts maternal health outcomes elevating the risk of maternal deaths after discharge. The accessibility and affordability of necessary prenatal care is integral to the field of health across the lifespan. Socioeconomic barriers within rural locations place expecting mothers at an adverse risk of developing conditions including preeclampsia and other complications that may result in hospital readmission. Economic barriers including hospital closures, shortages of specialty healthcare providers, unstable internet access and, ease of care access on further contribute to this disparity among rural women.

A retrospective analysis was conducted using data from Health Care Cost and …

Methionyl-Trna Synthetase Synthetic And Proofreading Activities Are Determinants Of Antibiotic Persistence, Whitney N. Wood, Miguel Angel Rubio, Lorenzo Eugenio Leiva, Gregory J. Phillips, Michael Ibba

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Bacterial antibiotic persistence is a phenomenon where bacteria are exposed to an antibiotic and the majority of the population dies while a small subset enters a low metabolic, persistent, state and are able to survive. Once the antibiotic is removed the persistent population can resuscitate and continue growing. Several different molecular mechanisms and pathways have been implicated in this phenomenon. A common mechanism that may underly bacterial antibiotic persistence is perturbations in protein synthesis. To investigate this mechanism, we characterized four distinct metG mutants for their ability to increase antibiotic persistence. Two metG mutants encode changes near the catalytic site …

Examining Childhood Trauma And Substance Use Problems Among Justice-Involved Adolescents In Florida, Skye C. Bristol

USF Tampa Graduate Theses and Dissertations

The purpose of this dissertation was to assess the relationship between childhood trauma and substance use problems as latent constructs among justice-involved adolescents (JIA). Additionally, racial differences and spatial analyses were examined. Previous research underscores the limitations of conceptualizing childhood trauma solely through Adverse Childhood Experiences (ACEs). This justified integrating child welfare involvement, or out-of-home placement, as an additional indicator to develop a comprehensive scope of childhood trauma among JIA. Furthermore, current literature established that childhood trauma is a key predictor of substance misuse among JIA. However, the lack of a standardized measure of substance misuse across juvenile justice systems …

Ly6c+ Monocytes In The Skin Promote Systemic Alphavirus Dissemination, Autumn C Holmes, Cormac J Lucas, Morgan E Brisse, Brian C Ware, Heather D Hickman, Thomas E Morrison, Michael S Diamond

2020-Current year OA Pubs

Alphaviruses are mosquito-transmitted pathogens that induce high levels of viremia, which facilitates dissemination and vector transmission. One prevailing paradigm is that, after skin inoculation, alphavirus-infected resident dendritic cells migrate to the draining lymph node (DLN), facilitating further rounds of infection and dissemination. Here, we assess the contribution of infiltrating myeloid cells to alphavirus spread. We observe two phases of virus transport to the DLN, one that occurs starting at 1 h post infection and precedes viral replication, and a second that requires replication in the skin, enabling transit to the bloodstream. Depletion of Ly6C

Impact Of Imaging Frequency On Progression-Free Survival In Alliance Trials Enrolling Patients With Follicular Lymphoma, Sarah C Rutherford, Jun Yin, Levi D Pederson, Kristie A Blum, Peter Martin, Sin-Ho Jung, Barbara Grant, Cara Rosenbaum, Bruce D Cheson, Nancy L Bartlett, Sumithra J Mandrekar, John P Leonard

2020-Current year OA Pubs

No abstract provided.

Older Adults Are At Greater Risk Of Opioid Use Disorder In Communities With High Social Vulnerability, Tse-Chuan Yang, Seulki Kim, Stephen A. Matthews, Carla Shoff

Population Health Research Brief Series

The prevalence of opioid use disorder (OUD) among older adults has increased and imposes a heavy burden on the health care system in the United States. This brief summarizes findings from a study that used data from the Centers for Medicare and Medicaid Services to examine how county characteristics contributed to the prevalence of OUD among Medicare beneficiaries age 65+ in U.S. counties in 2021. They found that the prevalence of OUD among these adults is higher in counties with more place-level social vulnerability (e.g., socioeconomic disadvantage, high housing cost burden).

Phase 2 Study Of Add-On Parsaclisib For Patients With Myelofibrosis And Suboptimal Response To Ruxolitinib: Final Results, Abdulraheem Yacoub, Stephen T Oh, Et Al.

2020-Current year OA Pubs

Ruxolitinib reduces spleen volume, improves symptoms, and increases survival in patients with intermediate- or high-risk myelofibrosis. However, suboptimal response may occur, potentially because of signaling via the phosphoinositide 3-kinase (PI3K)/protein kinase B pathway. This phase 2 study evaluated dosing, efficacy, and safety of add-on PI3Kδ inhibitor parsaclisib for patients with primary or secondary myelofibrosis with suboptimal response to ruxolitinib. Eligible patients remained on a stable ruxolitinib dose and received add-on parsaclisib 10 or 20 mg, once daily for 8 weeks, and once weekly thereafter (daily-to-weekly dosing; n = 32); or parsaclisib 5 or 20 mg, once daily for 8 weeks, …

Efficacy And Feasibility Of Short-Stretch Compression Therapy For Filarial Lymphedema In Sri Lanka, Jaimee M Hall, Nirmitha Lalindi De Silva, Janaka Ruben, Sandani S Thilakarathne, Thishan Channa Yahathugoda, Philip J Budge

2020-Current year OA Pubs

The WHO-recommended essential package of care (EPC) for filarial limb lymphedema consists of daily limb washing, entry lesion management, limb protection, exercises, and elevation. Decongestive therapy (DT) with compression bandaging by trained lymphedema therapists adds additional benefit but is unavailable for most in low- and middle-income countries (LMICs). To determine whether DT using self-adjustable, short-stretch compression garments (SSCG), prefitted using portable, three-dimensional infrared imaging (3DII), would be effective and feasible in LMIC settings, we conducted a pilot 6-week, interventional, single-group, open-label pilot study in Galle, Sri Lanka. Ten participants with Dreyer stage 3 lymphedema used SSCG for 2 weeks after …

Articulation Versus Motor Speech? Supervisor Perspectives Of Slp Graduate Students: A Qualitative Study, Maddison Verser

Student Theses and Dissertations

This qualitative study was to determine clinical supervisors' perceptions of graduate students’ skill set of articulation disorders compared to motor speech disorders. Participants in this study included SLP clinical supervisors of Master’s level graduate students in Communication Disorders across various pediatric settings, including private practice, school-based or outpatient clinics in north central Arkansas. Face-to-face/phone interviews were recorded with a digital recorder and transcribed using the speech-to-text transcription service at Temi.com. The data were analyzed by the primary investigator to identify concepts and themes. The results of this study showed three main themes: (1) students are perceived as more independent and …

Hearing Loss Vs Length Of Employment In The Steel Industry, Sarah Jane Hattle

Student Theses and Dissertations

The purpose of this research was to investigate if there was a relationship between hearing loss and length of employment in the steel industry. Participants included 11 males that worked in the steel industry. Participants completed questionnaires regarding demographics, employment, and noise exposure, as well as took part in an audiological exam. The results of the audiological evaluations showed that 45% of participants had hearing loss in the right ear, 36% of participants had hearing loss in the left ear, and 36% of participants had loss in both ears. While there was no significant relationship between hearing loss and the …

Raf Inhibitor Re-Challenge Therapy In Braf-Aberrant Pan-Cancers: The Re-Raffle Study, Blessie Elizabeth Nelson, Jason Roszik, Jibran Ahmed, Carmelia Maria Noia Barretto, Mirella Nardo, Erick Campbell, Amber M Johnson, Sarina A Piha-Paul, Isabella C Glitza Oliva, Shiao-Pei Weathers, Maria Cabanillas, Milind Javle, Funda Meric-Bernstam, Vivek Subbiah

Faculty, Staff and Student Publications

Previous studies have shown the clinical benefit of rechallenging the RAF pathway in melanoma patients previously treated with BRAF inhibitors. 44 patients with multiple tumors harboring RAF alterations were rechallenged with a second RAF inhibitor, either as monotherapy or in combination with other therapies, after prior therapy with a first RAF inhibitor. This retrospective observational study results showed that rechallenging with RAFi(s) led to an overall response rate of 18.1% [PR in thyroid (1 anaplastic; 3 papillary), 1 ovarian, 2 melanoma, 1 cholangiocarcinoma, and 1 anaplastic astrocytoma]. The clinical benefit rate was 54.5%; more than 30% of patients had durable …

The Effect Of Virtual Standardized Patient Integrated Health Coach Training On Professional Confidence Gain In Veterans Health Administration Primary Care Nurses, David Mullins

Graduate Theses, Dissertations, and Capstones

Poor health behaviors cost the United States billions of dollars and tens of thousands of lives every year. The Veterans Affairs primary care delivery model uses health coaching as a catalyst to improve health behaviors in veterans. Health coaching is a complex skill that requires specialized training, repetitive practice, and professional confidence. Albert Bandura believed most people acquire skills and develop the confidence to use those skills through social learning. He posited that professional confidence played a critical role in skill acquisition, and people with low professional confidence were less likely to attempt to perform a skill. Bandura’s Theory of …

Biomarkers For Managing Neurodegenerative Diseases, Lara Cheslow, Adam E. Snook, Scott A. Waldman

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

Neurological disorders are the leading cause of cognitive and physical disability worldwide, affecting 15% of the global population. Due to the demographics of aging, the prevalence of neurological disorders, including neurodegenerative diseases, will double over the next two decades. Unfortunately, while available therapies provide symptomatic relief for cognitive and motor impairment, there is an urgent unmet need to develop disease-modifying therapies that slow the rate of pathological progression. In that context, biomarkers could identify at-risk and prodromal patients, monitor disease progression, track responses to therapy, and parse the causality of molecular events to identify novel targets for further clinical investigation. …

Hesitation Towards The Covid-19 Vaccine In The United States: A Digital Ethnographic Study [Vacilación Ante La Vacuna Contra El Covid-19 En Estados Unidos De América: Un Estudio Etnográfico Digital], Rosalynn A. Vega

Anthropology Faculty Publications

Following the authorization of the use of COVID-19 vaccines in babies age 6 months through children 4 years old in the United States, some individuals (parents, pediatricians, and communicators) framed COVID-19 vac-cination as an issue of access, while many others expressed hesitancy, and some resisted recommendations from the US Centers for Disease Control and Prevention. In this context, this study aimed to explore: 1) divergent reactions to the authorization of COVID-19 vaccine use in children aged 6 months to 4 years; and 2) opposing logics underlying attitudes towards pro-vaccination, anti-vaccination, and vaccine hesitancy regarding COVID-19 vaccines. To achieve this, a …

Amino Acid Formula Induces Microbiota Dysbiosis And Depressive-Like Behavior In Mice, Ji Hu, Kaixin He, Yifei Yang, Chuan Huang, Yiping Dou, Hao Wang, Guorong Zhang, Jingyuan Wang, Chaoshi Niu, Guoqiang Bi, Lan Zhang, Shu Zhu

Faculty, Staff and Student Publications

Amino acid formula (AAF) is increasingly consumed in infants with cow's milk protein allergy; however, the long-term influences on health are less described. In this study, we established a mouse model by subjecting neonatal mice to an amino acid diet (AAD) to mimic the feeding regimen of infants on AAF. Surprisingly, AAD-fed mice exhibited dysbiotic microbiota and increased neuronal activity in both the intestine and brain, as well as gastrointestinal peristalsis disorders and depressive-like behavior. Furthermore, fecal microbiota transplantation from AAD-fed mice or AAF-fed infants to recipient mice led to elevated neuronal activations and exacerbated depressive-like behaviors compared to that …

Nurses’ Attitudes And Knowledge Differences Toward Pain In Patients With Sickle Cell Disease, Peggy Boyd

Walden Dissertations and Doctoral Studies

No abstract provided.

Exploring The Associations Between Drug Resistant Mutations, Low-Level Viremia, And Virological Failure In Persons With Hiv In Florida, Adriana Campos

USF Tampa Graduate Theses and Dissertations

Virologic suppression is an essential part to a successful regimen for Persons with HIV (PWH) and the 95-95-95 initiative. Yet, PWH experiencing episodes of low-level viremia (LLV) may be more prone to experience virological failure (VF). Currently, studies debate the clinical relevance of LLV and its effect on virological outcomes, but none have explored its interconnectedness with drug resistant mutations (DRM) and antiretroviral treatment (ART) adherence – all integral elements in predicting virological outcomes.

The goal of the proposed study was to contribute to the knowledge gap regarding the interconnectivity of drug resistant mutations, low-level viremia, adherence, and virological failure …

Community-Centered Early Warning For Outbreaks In Humanitarian Emergencies: Evaluating Community-Based Surveillance For Priority Communicable Diseases Among Internally Displaced Populations In Iraq, Caitlin M. Wolfe

USF Tampa Graduate Theses and Dissertations

Background: Community based surveillance (CBS) is the organized and rapid collection of information from health events identified in the community and includes the systematic detection and reporting of events of public health significance. While used globally to enhance the detection of priority diseases, CBS has not been routinely deployed in Iraq, where a once-robust health system has been devastated by decades of war, international sanctions, and large-scale population displacement. During infectious disease outbreaks, it is imperative to quickly detect and isolate suspect cases, investigate confirmed cases, and trace and monitor the contacts of confirmed cases. The COVID-19 pandemic underscored the …

Correction: The Novel Phosphatase Nudt5 Is A Critical Regulator Of Triple-Negative Breast Cancer Growth, Jing Qian, Yanxia Ma, William M Tahaney, Cassandra L Moyer, Amanda Lanier, Jamal Hill, Darian Coleman, Negar Koupaei, Susan G Hilsenbeck, Michelle I Savage, Brent D G Page, Abhijit Mazumdar, Powel H Brown

Faculty, Staff and Student Publications

No abstract provided.

Effect Of Early Vs Late Inguinal Hernia Repair On Serious Adverse Event Rates In Preterm Infants: A Randomized Clinical Trial, Martin L Blakely, Andrea Krzyzaniak, Melvin S Dassinger, Claudia Pedroza, Jorn-Hendrik Weitkamp, Ankush Gosain, Michael Cotten, Susan R Hintz, Henry Rice, Sherry E Courtney, Kevin P Lally, Namasivayam Ambalavanan, Catherine M Bendel, Kim Chi T Bui, Casey Calkins, Nicole M Chandler, Roshni Dasgupta, Jonathan M Davis, Katherine Deans, Daniel A Deugarte, Jeffrey Gander, Carl-Christian A Jackson, Martin Keszler, Karen Kling, Stephen J Fenton, Kimberley A Fisher, Tyler Hartman, Eunice Y Huang, Saleem Islam, Frances Koch, Shabnam Lainwala, Aaron Lesher, Monica Lopez, Meghna Misra, Jamie Overbey, Brenda Poindexter, Robert Russell, Steven Stylianos, Douglas Y Tamura, Bradley A Yoder, Donald Lucas, Donald Shaul, P Ben Ham, Colleen Fitzpatrick, Kara Calkins, Aaron Garrison, Diomel De La Cruz, Shahab Abdessalam, Charlotte Kvasnovsky, Bradley J Segura, Joel Shilyansky, Lynne M Smith, Jon E Tyson

Faculty, Staff and Student Publications

Importance: Inguinal hernia repair in preterm infants is common and is associated with considerable morbidity. Whether the inguinal hernia should be repaired prior to or after discharge from the neonatal intensive care unit is controversial.

Objective: To evaluate the safety of early vs late surgical repair for preterm infants with an inguinal hernia.

Design, setting, and participants: A multicenter randomized clinical trial including preterm infants with inguinal hernia diagnosed during initial hospitalization was conducted between September 2013 and April 2021 at 39 US hospitals. Follow-up was completed on January 3, 2023.

Interventions: In the early repair strategy, infants underwent inguinal …

Author Correction: Cytoskeleton Remodeling Induced By Smyd2 Methyltransferase Drives Breast Cancer Metastasis, Alexandre G Casanova, Gael S Roth, Simone Hausmann, Xiaoyin Lu, Ludivine J M Bischoff, Emilie M Froeliger, Lucid Belmudes, Ekaterina Bourova-Flin, Natasha M Flores, Ana Morales Benitez, Tourkian Chasan, Marcello Caporicci, Jessica Vayr, Sandrine Blanchet, Francesco Ielasi, Sophie Rousseaux, Pierre Hainaut, Or Gozani, Muriel Le Romancer, Yohann Couté, Andres Palencia, Pawel K Mazur, Nicolas Reynoird

Faculty, Staff and Student Publications

No abstract provided.

Integration Of Kupffer Cells Into Human Ipsc-Derived Liver Organoids For Modeling Liver Dysfunction In Sepsis, Yang Li, Yunzhong Nie, Xia Yang, Yang Liu, Xiaoshan Deng, Yoshihito Hayashi, Riana Plummer, Qinglin Li, Na Luo, Toshiharu Kasai, Takashi Okumura, Yu Kamishibahara, Takemasa Komoto, Takuya Ohkuma, Satoshi Okamoto, Yumiko Isobe, Kiyoshi Yamaguchi, Yoichi Furukawa, Hideki Taniguchi

Faculty, Staff and Student Publications

Maximizing the potential of human liver organoids (LOs) for modeling human septic liver requires the integration of innate immune cells, particularly resident macrophage Kupffer cells. In this study, we present a strategy to generate LOs containing Kupffer cells (KuLOs) by recapitulating fetal liver hematopoiesis using human induced pluripotent stem cell (hiPSC)-derived erythro-myeloid progenitors (EMPs), the origin of tissue-resident macrophages, and hiPSC-derived LOs. Remarkably, LOs actively promote EMP hematopoiesis toward myeloid and erythroid lineages. Moreover, supplementing with macrophage colony-stimulating factor (M-CSF) proves crucial in sustaining the hematopoietic population during the establishment of KuLOs. Exposing KuLOs to sepsis-like endotoxins leads to significant …

A Novel Machine Learning Algorithm Selects Proteome Signature To Specifically Identify Cancer Exosomes, Bingrui Li, Fernanda G Kugeratski, Raghu Kalluri

Faculty, Staff and Student Publications

Non-invasive early cancer diagnosis remains challenging due to the low sensitivity and specificity of current diagnostic approaches. Exosomes are membrane-bound nanovesicles secreted by all cells that contain DNA, RNA, and proteins that are representative of the parent cells. This property, along with the abundance of exosomes in biological fluids makes them compelling candidates as biomarkers. However, a rapid and flexible exosome-based diagnostic method to distinguish human cancers across cancer types in diverse biological fluids is yet to be defined. Here, we describe a novel machine learning-based computational method to distinguish cancers using a panel of proteins associated with exosomes. Employing …