Medicine and Health Sciences Commons^™

Batf Is A Major Driver Of Nk Cell Epigenetic Reprogramming And Dysfunction In Aml, Bijender Kumar, Anand Singh, Rafet Basar, Nadima Uprety, Ye Li, Huihui Fan, Ana Karen Nunez Cortes, Mecit Kaplan, Sunil Acharya, Hila Shaim, Anna C Xu, Manrong Wu, Emily Ensley, Dexing Fang, Pinaki P Banerjee, Luciana Melo Garcia, Silvia Tiberti, Paul Lin, Hind Rafei, Maliha Nuzhat Munir, Madison Moore, Mayra Shanley, Mayela Mendt, Lucila N Kerbauy, Bin Liu, Alexander Biederstädt, Elif Gokdemir, Susmita Ghosh, Kiran Kundu, Francia Reyes-Silva, Xin Ru Jiang, Xinhai Wan, April L Gilbert, Merve Dede, Vakul Mohanty, Jinzhuang Dou, Patrick Zhang, Enli Liu, Luis Muniz-Feliciano, Gary M Deyter, Abhinav K Jain, Juan Jose Rodriguez-Sevilla, Simona Colla, Guillermo Garcia-Manero, Elizabeth J Shpall, Ken Chen, Hussein A Abbas, Kunal Rai, Katayoun Rezvani, May Daher

Faculty, Staff and Student Publications

Myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) belong to a continuous disease spectrum of myeloid malignancies with poor prognosis in the relapsed/refractory setting necessitating novel therapies. Natural killer (NK) cells from patients with myeloid malignancies display global dysfunction with impaired killing capacity, altered metabolism and exhausted phenotype at the single cell transcriptomic and proteomic levels. In this study we identified that this dysfunction was mediated through a crosstalk between NK cells and myeloid blasts necessitating cell-cell contact. NK cell dysfunction could be prevented by targeting the αvβ integrin/TGF-β/SMAD pathway but, once established, was persistent due to profound epigenetic reprogramming. …

Constitutive Upregulation Of Transcription Factors Underlies Permissive Bradyzoite Differentiation In A Natural Isolate Of Toxoplasma Gondii, Jing Xia, Yong Fu, Wanyi Huang, Taher Uddin, L David Sibley

2020-Current year OA Pubs

UNLABELLED:

IMPORTANCE:

Adverse Cardiovascular And Kidney Outcomes In People With Sars-Cov-2 Treated With Sglt2 Inhibitors, Taeyoung Choi, Yan Xie, Ziyad Al-Aly

2020-Current year OA Pubs

BACKGROUND: Whether use of SGLT2 inhibitors reduces the risk of cardiovascular and kidney events in people who contracted SARS-CoV-2 infection is not clear.

METHODS: We used the healthcare databases of the United States Department of Veterans Affairs to build a cohort of 107,776 participants on antihyperglycemic therapy and had SARS-CoV-2 infection between March 01, 2020 and June 10, 2023. Within them, 11,588 used SGLT2 inhibitors and 96,188 used other antihyperglycemics. We examined the risks of major adverse cardiovascular events (MACE)-a composite of death, myocardial infarction and stroke, and major adverse kidney events (MAKE)-a composite of death, eGFR decline > 50%, and …

Prenatal Environment Is Associated With The Pace Of Cortical Network Development Over The First Three Years Of Life, Ursula A Tooley, Aidan Latham, Jeanette K Kenley, Dimitrios Alexopoulos, Tara A Smyser, Ashley N Nielsen, Lisa Gorham, Barbara B Warner, Joshua S Shimony, Jeffrey J Neil, Joan L Luby, Deanna M Barch, Cynthia E Rogers, Christopher D Smyser

2020-Current year OA Pubs

Environmental influences on brain structure and function during early development have been well-characterized, but whether early environments are associated with the pace of brain development is not clear. In pre-registered analyses, we use flexible non-linear models to test the theory that prenatal disadvantage is associated with differences in trajectories of intrinsic brain network development from birth to three years (n = 261). Prenatal disadvantage was assessed using a latent factor of socioeconomic disadvantage that included measures of mother's income-to-needs ratio, educational attainment, area deprivation index, insurance status, and nutrition. We find that prenatal disadvantage is associated with developmental increases in …

Diagnostic Utility Of Brain Mri Volumetry In Comparing Traumatic Brain Injury, Alzheimer Disease And Behavioral Variant Frontotemporal Dementia, Cyrus A Raji, Somayeh Meysami, Verna R Porter, David A Merrill, Mario F Mendez

2020-Current year OA Pubs

BACKGROUND: Brain MRI with volumetric quantification, MRI volumetry, can improve diagnostic delineation of patients with neurocognitive disorders by identifying brain atrophy that may not be evident on visual assessments.

OBJECTIVE: To investigate diagnostic utility of MRI volumetry in traumatic brain injury (TBI), early-onset Alzheimer disease (EOAD), late-onset Alzheimer disease, and behavioral variant frontotemporal dementia (bvFTD).

METHOD: We utilized 137 participants of TBI (n = 40), EOAD (n = 45), LOAD (n = 32), and bvFTD (n = 20). Participants had 3D T1 brain MRI imaging amendable to MRI volumetry. Scan volumes were analyzed with Neuroreader. One-way ANOVA compared brain volumes …

Birth Defect Trends Within Texas Public Health Region 11, 2000-2019: An Analysis Of Texas Department Of State Health Services Public Data, Jonathan M. Hebert, Miguel A. Lopez, Padmanabhan Rengasamy

Research Colloquium

Background: South Texas is a medically underserved area with pockets of low socioeconomic status and poor access to healthcare, including prenatal care. This paper aims to monitor the changes in prevalence for cardiac, circulatory, gastrointestinal, genitourinary, and neural tube birth defects in this region in the last two decades to monitor the effectiveness of current public health efforts.

Methods: Public data on 20 birth defects from the Texas Department of State Health Services were obtained for decades 2000-2010 and 2010-2019 in Texas Public Health Region 11 and the remaining regions of Texas. Prevalences and odds ratios for all birth defects …

Atypical Clinical Presentation Of Murine Typhus Infection In Relative Immunosuppressed Patient In South Texas, Johanna Mora, Kirhyn Stein, Hernan Guanajuato, Sobia Memon, Laura Garcia, Jose Campo Maldonado

Research Colloquium

Background: Murine typhus, caused by Rickettsia typhi, is a zoonotic infection transmitted to humans via fleas. It typically begins with nonspecific symptoms such as fever, headache, and nausea. Most cases resolve without complications after a 7–14-day incubation period. The primary reservoirs are rats, opossums, cats, and dogs. This case highlights an atypical presentation of murine typhus in an immunosuppressed patient in South Texas.

Case Presentation: 34-year-old female with ankylosing spondylitis treated recently with secukinumab presented to her Primary Care Physician (PCP) due to fevers. PCP ordered laboratory workup which was unrevealing. Four days later, the patient went to the …

Assessing The Impact Of An Educational Healthcare Competition In Enhancing Student Knowledge Of Public Health, Alyssa L. Sepulveda, Ronald A. Shaju, Nura Salhadar, Brianna Guillen, Alexa Zarate

Research Colloquium

Background: Educational disparities have a huge impact on unequal healthcare outcomes. Students from underserved areas often lack awareness of healthcare disparities impacting their communities. This study aimed to develop and assess the effectiveness of a program conducted by medical students to enhance high school students’ understanding of these disparities in their home, the Rio Grande Valley.

Methods: The Education Collaboration for Aspiring Health Professionals (ECAHP) competition is a one-day annual program. It is designed and implemented by UTRGV medical students in collaboration with the School of Medicine’s Area Health Education Center Program (AHEC). The program targets high school students …

Assessing The Impact Of A Comprehensive Care Model On Reducing A1c Levels Compared To Primary Care Alone: A Comprehensive Study, Hector Trejo, Lizette Ingle, Karla Soberanis

Research Colloquium

Introduction: Diabetes significantly challenges the Rio Grande Valley (RGV), especially in low-income rural areas where prevalence and mortality rates are higher than in urban regions. Limited access to routine healthcare and specialists exacerbates these issues, delaying medical attention and reducing diagnostic testing and management adherence. Early diagnosis and lifestyle modification interventions show promise, but objective evaluation of these models by local organizations in the RGV. Further analysis is needed to assess their effectiveness in reducing diabetes prevalence and improving health outcomes in the RGV.

Methods: This retrospective study aims to evaluate the Comprehensive Care Model (CCM) on Improved Health Outcomes …

Assessing Preparedness, Attitudes, And Knowledge Of Knapp-Utrgv Family Medicine Residents On Geriatric Nursing Home Care: Phase 1 - Creation Of Interactive Modules, Maria M. Ferreira Caceres, Miguel A. Alvarez Silva, Juan C. Lopez-Alvarenga

Research Colloquium

Background: Training in geriatrics and nursing home care are a critical element of medical education. This gap in knowledge becomes increasingly significant given the changing demographics and the need to provide adequate care for an aging population. Improving geriatrics curriculum and implementing nursing home rotations are essential to ensure optimal care for the elderly, thereby fulfilling both educational and healthcare imperatives.

Objective: We aim to analyze and assess the influence of geriatrics training through a survey to collect attitudes, skills, and knowledge of UTRGV Knapp Family Medicine residents. Additionally, the goal is to create interactive modules to boost their skills …

Assessing Awareness, Knowledge, And Beliefs Regarding Opiates And Benzodiazepines And Deprescribing Protocols By Residents At Utrgv Knapp Family Medicine Residency Program., Ivana Y. Mangione Leon, Adrian Sandoval, Juan Lopez-Alvarenga, Gerardo Munoz-Monaco

Research Colloquium

Background: Controlled substances encompass drugs and specific chemicals subject to strict regulation due to their potential for abuse or risk. Opioid overdose remains a significant public health issue in the United States. Prescription opioids contribute to 18% of these deaths, while benzodiazepines are implicated in 31% to 61%. Clinicians encounter challenges in avoiding co-prescription, especially in patients with chronic pain and anxiety or sleep disorders. Despite growing interest in teaching mindful opioid prescribing, there is a lack of interventions to educate primary care clinicians and medical trainees on providing opioid overdose education and prevention. This quality improvement project aims to …

A Symphony Of Struggle: The Tender Connection Between Bad Health And Poverty, Jashkumar Choudhari

be Still

In the journey of crafting this essay, I sought to explore the profound connection between bad health and poverty, recognizing how these two facets of human existence intricately interlace, influencing and shaping one another in ways both profound and far-reaching. The process of delving into this theme was like unraveling a multi-layered tapestry, each thread revealing a new perspective on the unbreakable bond that exists between these seemingly distinct aspects of life.

Through the creative process, I aimed to capture the emotional resonance of this theme, evoking a sense of empathy and understanding in the reader. The imagery of haunting …

Evaluation Of The Influence Of Training In An Immersive Virtual Reality Environment On Sports Skills In Table Tennis, Martin Škopek, Josef Heidler, Štefan Balkó, Lenka Vojtíková, Radka Ulrichová

Baltic Journal of Health and Physical Activity

Introduction: This study explores the possibilities of leveraging immersive virtual reality (VR) training for the improvement of table tennis skills. The primary objective was to assess the in-fluence of VR intervention on the development of table tennis skills. Materials and Methods: Thirty-eight participants, whose mean age was 17.37 ± 0.84 years and who lacked previous table tennis experience, were divided into experimental (n = 21) and control (n = 17) groups. The experimental group engaged in six sessions of VR training aimed at enhancing table tennis skills, and their performance was evaluated using a comprehensive test battery. In contrast, the …

Chronic Guillain-Barré Syndrome From Misdiagnosed Lyme Disease, Carlos H. Cisneros Jr, Alberto E. Almeida

MEDI 9331 Scholarly Activities Clinical Years

Lyme disease (borreliosis) is caused by transmission of Borrelia burgdorferi from deer ticks in the United States. Neurological symptoms can manifest in the early disseminated stage of the disease and have the worst involvement in the late dissemination stage. Few, but important case reports exist of patients experiencing disseminated Lyme with associated Guillain-Barre Syndrome (GBS). GBS manifests as an ascending flaccid paralysis with additional sensory deficits and is characterized as an immune-mediated polyneuropathy attacking the peripheral nervous system. A college-aged, wheelchair bound, quadriplegic female presented to the clinic to establish care in October 2023. She reported being bitten by a …

Synthesis, Antiproliferative Activity And Molecular Docking Studies Of Neo‑ And Isocryptolepine Conjugates, Asmaa Taha

Nanotechnology Research Centre

A series of twelve conjugates 9a-c, 10a-c, 11a-c, and 12a-c based on naturally occurring neo- and isocryptolepines I and II were synthesized and characterized. The synthetic pathways involved nucleophilic substitution reaction of the preprepared amines 4a-c and 5a-c with 1-(4-bromobutyl)indole derivatives 8a and 8b, respectively, in aprotic solvent under heating. The structures of the synthesized conjugates were elucidated by FTIR, ¹H NMR, ¹³C NMR, and MS spectrometry and showed data consistence with the expected structures. The antiproliferative activity of 9a-c, 10a-c, 11a-c and 12a-c was evaluated against HepG-2 and HCT-116 cancer cell lines using normal …

The Effectiveness Of The Gnana Laryngeal Mask Airway Ii (Gla-Ii) With Novel Suction Tubing In Gastrointestinal (Gi) Cases, Shahab Ahmadzadeh, Landyn D. Johnson, William T. Barham, James Ilochi, Matthew Fredericks, Giustino Varrassi, Sahar Shekoohi, Alan D. Kaye

School of Medicine Faculty Publications

INTRODUCTION: The Gnana laryngeal mask airway II (GLA-II) is a supraglottic airway device similar to the classic laryngeal mask airway, except it has an additional suction port. This suction port allows for the removal of secretions and saliva. A previous version of the Gnana laryngeal airway 4 was made of silicone, while this newer version is made of polyvinyl chloride (PVC), which is more affordable. This study aimed to demonstrate the effectiveness and tolerability of this PVC-designed GLA-II and evaluate its ability to suction secretions. METHODS: The prospective cohort study included 100 gastrointestinal (GI) cases to determine the effectiveness and …

Non-Redundant Roles For The Human Mrna Decapping Cofactor Paralogs Dcp1a And Dcp1b, Ivana Vukovic, Samantha M. Barnada, Jonathan W. Ruffin, Jon Karlin, Ravi Kumar Lokareddy, Gino Cingolani, Steven B. Mcmahon

Department of Medicine Faculty Papers

Eukaryotic gene expression is regulated at the transcriptional and post-transcriptional levels, with disruption of regulation contributing significantly to human diseases. The 5' m7G mRNA cap is a central node in post-transcriptional regulation, participating in both mRNA stabilization and translation efficiency. In mammals, DCP1a and DCP1b are paralogous cofactor proteins of the mRNA cap hydrolase DCP2. As lower eukaryotes have a single DCP1 cofactor, the functional advantages gained by this evolutionary divergence remain unclear. We report the first functional dissection of DCP1a and DCP1b, demonstrating that they are non-redundant cofactors of DCP2 with unique roles in decapping complex integrity and specificity. …

Differences In Happiness And Perceived Meaning In Life Between U.S. Working-Age Adults With Versus Without A Self-Care Disability, Nastassia Vaitsiakhovich, Scott D. Landes, Shannon M. Monnat

Population Health Research Brief Series

Subjective wellbeing (SWB) - being happy or perceiving one’s life has meaning, is critical to good physical health. People who are happier and who report that their lives have meaning are healthier and live longer. In general, individuals with disabilities have worse SWB compared to those without disabilities. This brief summarizes findings from a study that used data from the National Wellbeing Survey collected in early-2021 to examine differences in happiness and perceived meaning in life between U.S. working-age adults (ages 18-64) with versus without a self-care disability (such as difficulty eating, using the toilet, or dressing without assistance) and …

Immunoactive Signatures Of Circulating Trna- And Rrna-Derived Rnas In Chronic Obstructive Pulmonary Disease, Megumi Shigematsu, Takuya Kawamura, Deepak Deshpande, Yohei Kirino

Computational Medicine Center Faculty Papers

Chronic obstructive pulmonary disease (COPD) is the most prevalent lung disease, and macrophages play a central role in the inflammatory response in COPD. We here report a comprehensive characterization of circulating short non-coding RNAs (sncRNAs) in plasma from patients with COPD. While circulating sncRNAs are increasingly recognized for their regulatory roles and biomarker potential in various diseases, the conventional RNA sequencing (RNA-seq) method cannot fully capture these circulating sncRNAs due to their heterogeneous terminal structures. By pre-treating the plasma RNAs with T4 polynucleotide kinase, which converts all RNAs to those with RNA-seq susceptible ends (5′-phosphate and 3′-hydroxyl), we comprehensively sequenced …

Hypercalcemia-Driven Spontaneous Tumor Lysis Syndrome In A Case Of Recently Diagnosed Multiple Myeloma: An Unusual Clinical Encounter, Edgar Dorsey Trevino, Taha Al Hassan, Olga G. Cantu-Rodriguez

School of Medicine Publications

Tumor lysis syndrome (TLS) represents an oncological emergency characterized by the rapid disintegration of neoplastic cells and subsequent release of their intracellular content into the systemic circulation due to cytotoxic therapy. However, a rare variant, spontaneous TLS (STLS), can occur without an evident precipitating factor. Prompt recognition of high-risk individuals and initiation of prophylactic interventions are crucial to forestall complications of electrolyte imbalance, such as cardiac arrhythmias or sudden death. We present the case of a 74-year-old male who was referred to the hematologist/oncologist’s office after exhibiting pelvic pain, progressive weakness, fatigue, unintended weight loss over the last five months, …

Neuroinflammation And Brain Health Risks In Veterans Exposed To Burn Pit Toxins, Athena Brooks, Brian Sandri, Joshua Nixon, Timothy Nurkiewicz, Paul Barach, Janeen Trembley, Tammy Butterick

College of Population Health Faculty Papers

Military burn pits, used for waste disposal in combat zones, involve the open-air burning of waste materials, including plastics, metals, chemicals, and medical waste. The pits release a complex mixture of occupational toxic substances, including particulate matter (PM), volatile organic compounds (VOCs), heavy metals, dioxins, and polycyclic aromatic hydrocarbons (PAHs). Air pollution significantly impacts brain health through mechanisms involving neuroinflammation. Pollutants penetrate the respiratory system, enter the bloodstream, and cross the blood-brain barrier (BBB), triggering inflammatory responses in the central nervous system (CNS). Chronic environmental exposures result in sustained inflammation, oxidative stress, and neuronal damage, contributing to neurodegenerative diseases and …

Pleural Effusions In Patients Diagnosed With Pneumonia In Urgent Care Clinics, Nathan C. Dean, Jacqueline R. Eve, Allison M. Butler, James H. Hart, Theadora Sakata, Karen E. Connor, Edward A. Stenehjem, Y.C.G. Lee

The University of Louisville Journal of Respiratory Infections

Introduction: Pleural effusions in patients with community-acquired pneumonia have been associated with worse outcomes in emergency department and hospital inpatient populations, but the incidence of effusions and their outcomes in outpatient urgent care clinics is unknown. This observational cohort study describes patients diagnosed with pneumonia and pleural effusions on upright 2-view chest X-rays performed in urgent care clinics.

Methods: We extracted electronic health record data from January 2019 through December 2020 on all patients over 12 years old with an International Statistical Classification of Diseases 10^th revision diagnosis of pneumonia entered by the treating clinician, plus “possible” or “likely” …

10-03-24 Hsc Committee Meeting Notice, Helen Maestas

HSC Committee (A subcommittee of the UNM Board of Regents)

10-03-24 HSC Committee Meeting Notice

Polyvinyl Alcohol Capped Silver Nanostructures For Fortified Apoptotic Potential Against Human Laryngeal Carcinoma Cells Hep-2 Using Extremely-Low Frequency Electromagnetic Field, Sara Abdelwahed, Hany Attia, Dalia El-Telbany, Mai Hamouda

Dentistry

Purpose: : Polyvinyl alcohol-capped silver nanostructures (cAgNSs) were investigated in order to enhance the cytotoxicity, proapoptotic, and oxidant patterns of in human laryngeal carcinoma Hep-2 cells by employing a 50 mT electromagnetic field (LEMF) for 30 min. Methods: Wet chemical reduction was used to synthesize the cAgNSs, and after they had been capped with polyvinyl alcohol, they were specifically examined for particle size analysis and structural morphology. To visualize how the silver may attach to the protein targets, a molecular docking study was conducted. Estimation of cytotoxicity, cell cycle progression supported by mRNA expression of three apoptotic-promoting genes and one …

Preferences For Attributes Of Oral Antipsychotic Treatments: Results From A Discrete-Choice Experiment In Respondents With Schizophrenia Or Bipolar I Disorder, Martha Sajatovic

Faculty Scholarship

Background: Antipsychotic medications are effective treatments for schizophrenia (SZ) and bipolar I disorder (BD-I), but when presented with different treatment options, there are tradeoffs that individuals make between clinical improvement and adverse effects. As new options become available, understanding the attributes of antipsychotic medications that are valued and the tradeoffs that individuals consider when choosing among them is important. Methods: A discrete-choice experiment (DCE) was administered online to elicit preferences across 5 attributes of oral antipsychotics: treatment efficacy (i.e., improvement in symptom severity), weight gain over 6 months, sexual dysfunction, sedation, and akathisia. Eligible respondents were aged 18–64 years with …

Inhibiting Endothelial Cell Mst1 Attenuates Acute Lung Injury In Mice, Zhi-Fu Guo, Nopprarat Tongmuang, Chao Li, Chen Zhang, Louis Hu, Daniel Capreri, Mei-Xing Zuo, Ross Summer, Jianxin Sun

Center for Translational Medicine Faculty Papers

Lung endothelium plays a pivotal role in the orchestration of inflammatory responses to acute pulmonary insults. Mammalian sterile 20-like kinase 1 (Mst1) is a serine/threonine kinase that has been shown to play an important role in the regulation of apoptosis, stress responses, and organ growth. This study investigated the role of Mst1 in lung endothelial activation and acute lung injury (ALI). We found that Mst1 was significantly activated in inflamed lung endothelial cells (ECs) and mouse lung tissues. Overexpression of Mst1 promoted nuclear factor κ-B (NF-κB) activation through promoting JNK and p38 activation in lung ECs. Inhibition of Mst1 by …

Targeting Prmt3 Impairs Methylation And Oligomerization Of Hsp60 To Boost Anti-Tumor Immunity By Activating Cgas/Sting Signaling, Yunxing Shi, Zongfeng Wu, Shaoru Liu, Dinglan Zuo, Yi Niu, Yuxiong Qiu, Liang Qiao, Wei He, Jiliang Qiu, Yunfei Yuan, Guocan Wang, Binkui Li

Faculty, Staff and Student Publications

Immune checkpoint blockade (ICB) has emerged as a promising therapeutic option for hepatocellular carcinoma (HCC), but resistance to ICB occurs and patient responses vary. Here, we uncover protein arginine methyltransferase 3 (PRMT3) as a driver for immunotherapy resistance in HCC. We show that PRMT3 expression is induced by ICB-activated T cells via an interferon-gamma (IFNγ)-STAT1 signaling pathway, and higher PRMT3 expression levels correlate with reduced numbers of tumor-infiltrating CD8+ T cells and poorer response to ICB. Genetic depletion or pharmacological inhibition of PRMT3 elicits an influx of T cells into tumors and reduces tumor size in HCC mouse models. Mechanistically, …

Reduce Unplanned Extubations In Seven Questions Or Less, Leslie Huntington

EBP Fellowship

No abstract provided.

Less Is More: Allele-Specific Removal Of Dysfunctional Ryr1 Channel Subunits, Derek Sun, William R Lagor

Faculty, Staff and Students Publications

No abstract provided.

Final Outcomes From A Phase 2 Trial Of Posoleucel In Allogeneic Hematopoietic Cell Transplant Recipients, Sanjeet S Dadwal, Rajat Bansal, Michael W Schuster, Jean A Yared, Gary Douglas Myers, Michelle Matzko, Sama Adnan, David Mcneel, Julie Ma, Sarah A Gilmore, Spyridoula Vasileiou, Ann M Leen, Joshua A Hill, Jo-Anne H Young

Faculty, Staff and Students Publications

Allogeneic hematopoietic cell transplantation (allo-HCT) recipients are susceptible to viral infections. We conducted a phase 2 trial evaluating the safety and rate of clinically significant infections (CSIs; viremia requiring treatment or end-organ disease) after infusion of posoleucel, a partially HLA-matched, allogeneic, off-the-shelf, multivirus-specific T-cell investigational product for preventing CSIs with adenovirus, BK virus, cytomegalovirus, Epstein-Barr virus, human herpesvirus-6, or JC virus. This open-label trial enrolled allo-HCT recipients at high risk based on receiving grafts from umbilical cord blood, haploidentical, mismatched, or matched unrelated donors; post-HCT lymphocytes of < 180/mm3; or use of T-cell depletion. Posoleucel dosing was initiated within 15 to 49 days of allo-HCT and subsequently every 14 days for up to 7 doses. The primary end point was the number of CSIs due to the 6 target viruses by week 14. Of the 26 patients enrolled, only 3 (12%) had a CSI by week 14, each with a single target virus. In vivo expansion of functional virus-specific T cells detected via interferon-γ enzyme-linked immunosorbent spot assay was associated with viral control. Persistence of posoleucel-derived T-cell clones for up to 14 weeks after the last infusion was confirmed by T-cell–receptor deep sequencing. Five patients (19%) had acute graft-versus-host disease grade 2 to 4. No patient experienced cytokine release syndrome. All 6 deaths were due to relapse or disease progression. allo-HCT recipients at high risk who received posoleucel had low rates of CSIs from 6 targeted viruses. Repeat posoleucel dosing was generally safe and well tolerated and associated with functional immune reconstitution.