Life Sciences Commons^™

Reliability Of Popliteal Artery Flow-Mediated Dilation In The Seated Position, Taskina Akhter

Rehabilitation Sciences Theses & Dissertations

Flow-mediated dilation (FMD) is a noninvasive measurement of endothelial function, which is a useful prognostic tool for cardiovascular disease risk. Despite its widespread use since 1992, the reproducibility of FMD varies widely between studies. This variability in reproducibility is especially significant in the case of the popliteal artery due to different methodological approaches. Studies perform popliteal FMD in various body positions, with the prone and seated positions most common. However, no studies have examined the reproducibility of both the seated and prone positions of the popliteal artery FMD. Therefore, the aim of this study is to examine the test-retest and …

Growing South Dakota (Spring 2024), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 3Empowering the Next Generation of Agriculture
[Page] 5 Celebration of Faculty Excellence
[Page] 7 Judging Teams Provide Connection, Critical Skills
[Page] 11 Melanie Caffe: SDSU Oat Breeder Finds Motivation in Making a Difference for Farmers
[Page] 13 2024 CAFES Outstanding Seniors
[Page] 14 SDSU Celebrates Students Accepted to Veterinary Schools through Stethoscope Ceremony"
[Page] 15 New Researchers in CAFES
[Page] 17 Hitting the Mark – Shooting Sports Driving Growth in SDSU Extension 4-H Program
[Page] 19 A New Century Leads to a New Barn
[Page] 20 CAFES News & Updates
[Page] 21 Jackrabbits Now & Then
[Page] 22 What …

Quantifying Costs And Benefits Of Alternative Overwintering Strategies In A Southeastern Population Of Timber Rattlesnakes (Crotalus Horridus), John Powers, Amber Stubbs, Alfred J. Mead, Dominic L. Desantis

Graduate Research Showcase

Temperate reptile populations are under strong selective pressure to minimize the costs of extreme winter temperatures. For high latitude and high elevation snake populations, this has often favored communal overwintering behavior, wherein large numbers of conspecifics aggregate at optimal sites given the limited availability of such features in the environment. Timber Rattlesnakes (Crotalus horridus) are distributed across a wide latitudinal gradient in North America, and therefore represent ideal models for exploring how variable winter selective regimes might favor different strategies. Here, we leverage a unique intermediate population of C. horridus from central Georgia, USA, that exhibits both communal …

Documenting The Southern Range Terminus Of The Wood Frog (Lithobates Sylvaticus) In North America, Christian Braswell

Theses

The Wood Frog (Lithobates sylvaticus) holds a remarkable position in North American amphibian biology, with its range extending from the Arctic Circle down to the near sub-tropical southeastern United States. This thesis presents a novel quantitative polymerase chain reaction analysis (qPCR) primer specific to L. sylvaticus and a survey effort regarding the southernmost distribution and detection of this species in Alabama through the application of environmental DNA (eDNA) sampling techniques. By investigating historical data and employing advanced genetic methodologies, this research provides insights into the contemporary status and distribution of the Wood Frog. This research is important to …

The Casnr L.I.N.K.S. Newsletter: Learning Innovation Network For K-12 Schools, Volume 3, Edition 4, April 2024, Bailey Feit, Tammy Mittelstet

CASNR L.I.N.K.S. Newsletter

The purpose of this L.I.N.K.S. newsletter is to provide a monthly update of opportunities and resources for K-12 schools to connect with the University of Nebraska's CASNR Food, Energy, Water, and Societal Systems (FEWS²) programs and partners.

Included in this edition:

K-12 curriculum and resources

Join us at Youth Energy Leadership Camp, July 22-26, 2024

Summer Discovery Day camp, Morrill Hall, June 3-August 2

Explore Food Science summer camps 2024

Save the date: Haskell Ag Lab family field day, August 7, 2024

Other upcoming events

Educator workshops

Compact Teacher Summit-Haskell Ag Lab, Wheat variety field day-High Plains Lab, NAYI conference-East …

How To Spark Bio Curiosity: An Innovative High School Biology Curriculum, Audrie Charles

Graduate Research Showcase

This project is a researched creative endeavor focused around the creation of an exemplary high school biology course. This presentation will showcase an original high school biology curriculum designed to ignite students’ innate curiosity and empower meaningful learning of core concepts like cell structure, genetics, evolution and ecology. Students revisit key ideas through a spiral curriculum that builds complexity, tackling open-ended problems and collaborating on hands-on inquiries like formulating hypotheses, designing experiments, collecting data and drawing evidence-based conclusions. Tangible projects allow students to demonstrate content knowledge in addition to critical thinking abilities like synthesizing concepts, evaluating claims and applying learning …

Turbidimetric Bioassays: A Solution To Antimicrobial Activity Detection In Asymptomatic Bacteriuria Isolates Against Uropathogenic Escherichia Coli, Ciara Kenneally, Craig P. Murphy, Roy D. Sleator, Eamonn P. Culligan

Department of Biological Sciences Publications

Traditional bacteriocin screening methods often face limitations due to diffusion-related challenges in agar matrices, which can prevent the peptides from reaching their target organism. Turbidimetric techniques offer a solution to these issues, eliminating diffusion-related problems and providing an initial quantification of bacteriocin efficacy in producer organisms. This study involved screening the cell-free supernatant (CFS) from eight uncharacterized asymptomatic bacteriuria (ABU) isolates and Escherichia coli 83972 for antimicrobial activity against clinical uropathogenic E. coli (UPEC) strains using turbidimetric growth methods. ABU isolates exhibiting activity against five or more UPEC strains were further characterized (PUTS 37, PUTS 58, PUTS 59, S-07-4, and …

Kinematics Of Bowing The Re-Strung Violin, Sarah Hayden

Master's Theses (2009 -)

Building upon existing research in motor learning and sensorimotor control, this thesis explores how the performance of a real-world task (playing a simple musical piece on the violin) is impacted by the spatial re-arrangement of the violin’s strings, and how performance recovers with practice (relearning). I recorded audio performances and bow kinematics as violinists with a wide range of prior skills played a familiar 2-octave G-Major arpeggio 50 times. Some subjects played all 50 arpeggios on a violin with the standard string arrangement (the control violin). Another set of subjects played the middle 30 arpeggios with a “similar” violin with …

Development And Validation Of A Vehicle Front Profile Finite Element Model To Evaluate Pedestrian Impacts, James Wolf

Master's Theses (2009 -)

In 2021, there were nearly 7500 pedestrians killed in traffic crashes in the U.S., the highest since 1981. Since the year 2000, Europe and Japan have adopted pedestrian crash protection programs for vehicles and have seen a decline in pedestrian fatalities; the U.S. has not yet adopted pedestrian crash protection and has seen an increase in pedestrian fatalities in that same time. European New Car Assessment Programme (Euro NCAP) outlines a detailed procedure for evaluations of new vehicles for pedestrian safety through headform, upper legform, and lower legform tests. Euro NCAP also uses generic finite element (FE) front-end vehicle models, …

Ecological Niche Modeling Of Endemic Texas Crayfish Species Under Current Conditions, Sarah A. Burgett

Biology Theses

Crayfish play many important roles in freshwater ecosystems such as ecosystem engineering, bioindicator, and food resource. Even though they are important to ecosystems, they are highly understudied. There has been previous work done on ecological niche modeling on Texas crayfish species, but this study takes it a step further by combining soil, climate, and hydrology variables to model the ecological niches of three endemic species: Procambarus texanus, Procambarus nueces, and Procambarus fayettei. Ecological niche models were created for the three species of interest using the program Maxent. I found that P. texanus and P. fayettei have indistinguishable …

Role Of Toll-Like Receptor 2 In Macrophage Recognition And Response To Borrelia Burgdorferi, Yukiye A. Koide

Master's Theses

Lyme disease is the most common vector-borne disease in the US and will experience an uptick as the insect host, the ixodid tick, gains habitat with climate change. The causative agent of Lyme disease, Borrelia burgdorferi, is recognized by Toll-like receptor 2 (TLR2), which initiates the innate immune response. Here, I used ELISA and SEAP detection assays to determine that knocking out TLR2 in THP-1 macrophages reduces the amount of NFkB activation, as well as IL-10 and IL-1β secretion. Then, I used fluorescence microscopy and Incucyte assays to quantify the amount of phagocytosis performed by wild-type and knock-out THP-1 cells. …

Did God Create Viruses?, James Hayward

Faculty Publications

No abstract provided.

Design, Protocol And Baseline Data Of Nurturing Healthy Teachers, A Cluster Non-Randomized Controlled Trial To Improve The Health, Well-Being, And Food Security Of Preschool And Elementary School Teachers In Houston, Texas, Shreela V Sharma, Mackenzie Senn, Angela Zieba, Miao Tang, Ru-Jye Chuang, Courtney Byrd-Williams, Mike Pomeroy, Azar Gaminian, Jill Cox, Katherine French, Nalini Ranjit

Journal Articles

BACKGROUND: We present the conceptual framework, design, and study measures of Nurturing Healthy Teachers, a quasi-experimental study to examine the short- and long-term effectiveness of the Nurturing Healthy Teachers (NHT) nutrition intervention on food insecurity, dietary behaviors, mental health and cardiometabolic health among preschool and elementary school teachers.

METHODS: A convenience sample of 28 elementary schools with pre-kindergarten and elementary classrooms were recruited in Houston, Texas. Nurturing Healthy Teacher intervention includes Brighter Bites, an evidence-based coordinated school health program that combines access to fresh produce and nutrition education, and Create Healthy Futures, a web-based nutrition education program that targets nutrition …

Adsorbents From Rice Husk And Shrimp Shell For Effective Removal Of Heavy Metals And Reactive Dyes In Water, Md Ibrahim H Mondal, Shovra Chandra Chakraborty, Md Saifur Rahman, Shaik Merkatur Hakim Marjuban, Firoz Ahmed, John L Zhou, Mohammad Boshir Ahmed, Masoumeh Zargar

Journal Articles

Widespread contamination by heavy metals (HMs) and dyes poses a major health risk to people and ecosystems requiring effective treatment. In this work, rice husk (RH) and shrimp shells were extracted to obtain amorphous silica and chitosan, respectively, which were utilized to produce nano-chitosan-coated silica (NCCS). To ensure the stability of the nanoparticles, silica was freeze-dried after being coated with nano-chitosan. Functional groups (-NH

Serum Metabolomic Markers Of Protein-Rich Foods And Incident Ckd: Results From The Atherosclerosis Risk In Communities Study, Lauren Bernard, Jingsha Chen, Hyunju Kim, Kari E Wong, Lyn M Steffen, Bing Yu, Eric Boerwinkle, Andrew S Levey, Morgan E Grams, Eugene P Rhee, Casey M Rebholz

Journal Articles

RATIONALE & OBJECTIVE: While urine excretion of nitrogen estimates the total protein intake, biomarkers of specific dietary protein sources have been sparsely studied. Using untargeted metabolomics, this study aimed to identify serum metabolomic markers of 6 protein-rich foods and to examine whether dietary protein-related metabolites are associated with incident chronic kidney disease (CKD).

STUDY DESIGN: Prospective cohort study.

SETTING & PARTICIPANTS: A total of 3,726 participants from the Atherosclerosis Risk in Communities study without CKD at baseline.

EXPOSURES: Dietary intake of 6 protein-rich foods (fish, nuts, legumes, red and processed meat, eggs, and poultry), serum metabolites.

OUTCOMES: Incident CKD (estimated …

A Multitrait Genetic Study Of Hemostatic Factors And Hemorrhagic Transformation After Stroke Treatment, Cristina Gallego-Fabrega, Gerard Temprano-Sagrera, Jara Cárcel-Márquez, Elena Muiño, Natalia Cullell, Miquel Lledós, Laia Llucià-Carol, Jesús M Martin-Campos, Tomás Sobrino, José Castillo, Mònica Millán, Lucía Muñoz-Narbona, Elena López-Cancio, Marc Ribó, Jose Alvarez-Sabin, Jordi Jiménez-Conde, Jaume Roquer, Silvia Tur, Victor Obach, Juan F Arenillas, Tomas Segura, Gemma Serrano-Heras, Joan Marti-Fabregas, Marimar Freijo-Guerrero, Francisco Moniche, Maria Del Mar Castellanos, Alanna C Morrison, Nicholas L Smith, Paul S De Vries, Israel Fernández-Cadenas, Maria Sabater-Lleal

Journal Articles

BACKGROUND: Thrombolytic recombinant tissue plasminogen activator (r-tPA) treatment is the only pharmacologic intervention available in the ischemic stroke acute phase. This treatment is associated with an increased risk of intracerebral hemorrhages, known as hemorrhagic transformations (HTs), which worsen the patient's prognosis.

OBJECTIVES: to investigate the association between genetically determined natural hemostatic factors' levels and increased risk of HT after r-tPA treatment.

METHODS: Using data from genome-wide association studies on the risk of HT after r-tPA treatment and data on 7 hemostatic factors (factor [F]VII, FVIII, von Willebrand factor [VWF], FXI, fibrinogen, plasminogen activator inhibitor-1, and tissue plasminogen activator), we performed …

Parasite Diversity Of The King Cobra (Ophiophagus Hannah), Indian Rat Snake (Ptyas Mucosa), And Spectacled Cobra (Naja Naja), Bailey Tator

ALL - Honors Theses

Thus far, there has been little research on the parasitic biodiversity in wild reptiles, particularly in India. Research has been done on the parasite diversity of certain species, such as the King cobra (Ophiophagus hannah) and the Spectacled cobra (Naja naja), however these samples were purely from captive animals (Rajesh, 2015). This gap in knowledge is important to address because of how critical parasites are to the ecosystem and understanding their host population (Hudson, 2006). Parasites are some of the most biodiverse organisms, yet there is little work done to describe them in biodiversity hotspots such as the Western Ghats …

Hilling As A Management Tactic And Tool For Understanding Soybean Gall Midge (Resseliella Maxima Gagné), Pragya Gupta

Department of Entomology: Dissertations, Theses, and Student Research

Soybean gall midge (Resseliella maxima Gagné, SGM) emerged as a new species in the United States Midwest in 2019, causing injury to soybean (Glycine max L.) Infestation of R. maxima starts with females laying eggs in the fissures or cracks that develop at the base of soybean during the V2-V3 growth stage. The emerged larvae feed on the stem internally, leading to wilting and death of the plants. With knowledge gaps in biology and behavior, management strategies for this pest are challenging. Hilling is a cultural practice causing soil to cover the base of stems and is …

Demographic Consequences Of Off-River Nesting For Piping Plover (Charadrius Melodus) And Interior Least Tern (Sternula Antillarum Athalassos) In The Lower Platte River System, Nebraska, Elsa M. Forsberg

School of Natural Resources: Dissertations, Theses, and Student Research

Piping plovers (Charadrius melodus; hereafter, plovers) and interior least terns (Sternula antillarum athalassos; hereafter, terns) are two avian species that nest along the Lower Platte River system (LPRS) in Nebraska. In the LPRS, river sandbars provide natural nesting habitat, but off-river sites provide substantial nesting habitat especially when sandbar habitat is scarce. Although presumed to be important for the persistence of plovers and terns, off-river habitat is not self-sustaining and predicted to decline. Understanding the vital rates of plovers and terns in the LPRS will inform the role of off- river sites in the future conservation …

Sustainable Living At Imsa, Vignesh Tiruvannamalai, Zuyu Liu

Goal 4: Intrapersonal Intelligence Narrative

Students will collect any recyclable materials they have discarded to create wing decorations in conjunction with everyone in the wing and learn about sustainability, including statistics and lifestyle guidance.

Experimental Test Of Reforestation With Maesopsis Eminii Engl. And Millettia Drastica Welw. In The Eco-Climatic Conditions Of Kinshasa In The Drc, Bozele Nyabobola Alphonse, Dande Assikpu Patrick, Belesi Katula Honore, Lubini Ayingweu Constantin

Journal of Bioresource Management

The study focuses on the the experimental test of reforestation with Maesopsis eminii and Millettia drastica in the eco-climatic conditions of Kinshasa, with the aim of assessing the development and growth of the species through germination, and their behaviour in relation to the soil in the conditions of Kinshasa, with a view to proposing advice for responsible afforestation and reforestation. The results show that both species tend to reduce soil acidity. After cultivation, the pHs were 4.49 and 4.69 respectively for soils 1 and 2, compared with 3.98 for soil 0. In addition, Millettia drastica neutralises aluminium in the soil …

Flora Of The Dreat Mountains (Center Of Algeria) - Inventory, Taxonomy And Biogeography, Nabila Ouadeh, Khellaf Rebbas

Journal of Bioresource Management

Following the publication of the article on the synthaxonomic study of the Dreat forest, the aim of this inventory of the Dreat forest’s flora (semi-arid zone in central Algeria) to allow us a quantitative analysis on the basis of biogeographic parameters such as specific richness, geography of distribution, endemism and rarity. A subjective sampling was carried out between 2018 and 2021 at the level of the various formations (forest, matorral, altitude grasslands, and steppe). This analysis showed considerable species richness, estimated at around 468 taxa belonging to 278 genera and 66 families. Therophytes dominate the other living forms, according to …

Climate Change-Associated Declines In Water Clarity Impair Feeding By Common Loons, Walter H. Piper, Max R. Glines, Kevin C. Rose

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Climate change has myriad impacts on ecosystems, but the mechanisms by which it affects individual species can be difficult to pinpoint. One strategy to discover such mechanisms is to identify a specific ecological factor related to survival or reproduction and determine how that factor is affected by climate. Here we used Landsat imagery to calculate water clarity for 127 lakes in northern Wisconsin from 1995 to 2021 and thus investigate the effect of clarity on the body condition of an aquatic visual predator, the common loon (Gavia immer). In addition, we examined rainfall and temperature as potential predictors …

A Comparison Of In Vitro Studies Between Cobalt(Iii) And Copper(Ii) Complexes With Thiosemicarbazone Ligands To Treat Triple Negative Breast Cancer, Duaa R. Alajroush, Chloe B. Smith, Brittney F. Anderson, Ifeoluwa T. Oyeyemi, Stephen J. Beebe, Alvin A. Holder

Undergraduate Research Symposium

Triple negative breast cancer (TNBC) is one of the most aggressive forms of breast cancer, and disproportionately affects African American women. TNBC cells lack the common hormone receptors that many pre-existing cancer treatments target. Fortunately, metal-based complexes with thiosemicarbazone ligands have gained significant attention for their potential as anti-cancer agents. Cobalt(III) complex ([Co(phen)2(MeATSC)](NO3)3•1.5H2O•C2H5OH]) and Copper(II) complex ([Cu(acetylethTSC)Cl]Cl•0.25C2H5OH) specifically have properties of high toxicity, which can contribute to decreased cancer cell activity. The effects of these complexes are currently being investigated on cancerous and non-cancerous breast cell lines. The cytotoxic effect of the cobalt(lll) complex and the copper(ll) complex was analyzed …

Ornithofauna Diversity Of Tehsil Pakpattan, Punjab, Pakistan, Muhammad Amir Sial

Journal of Bioresource Management

Tehsil Pakpattan is situated in the Doab region of Punjab, Pakistan, along the Sutlej River. The present study aimed to evaluate the avian biodiversity of Tehsil Pakpattan. The study included the evaluation of species diversity, IUCN Red List status, migratory description, and feeding habits of the bird populations of Tehsil Pakpattan. Regular surveys were conducted at ten different localities in Tehsil Pakpattan, including Islam Colony, Kanipur, Farid Kot, 14 S/P, Chak Khagga, Musewal, 8 S/P, Noorpur, Green Town, and Bonga Niaz Khan, from January 2022 to April 2023. The Shannon‒Wiener diversity index value (R' = 3.41) indicated the notable diversity …

Assessing The Effectiveness Of Copper Oxide Nanoparticles (Cuo-Nps) As Larvicidal Agents Against Aedes Aegypti Larvae In A Laboratory Setup, Muhammad Ahsan Riaz, Ayesha Riaz, Muhammad Asif Mahmood, Muhammad Uzair Mukhtar, Waseem Akram, Ehtisham Arshad, Zaib Un Nisa, Beenish Ijaz, Muhammad Shahid Rasool

Journal of Bioresource Management

The control of Aedes aegypti (A. aegypti), the primary vector for dengue virus, requires effective larvicidal agents to target larvae breeding in various water sources. This study aimed to assess the effectiveness of Copper Oxide Nanoparticles (CuO-NPs) as larvicidal agents against A. aegypti larvae in a laboratory setup. Larvae were exposed to CuO-NPs at five contrasting concentrations (0, 5, 10, 20, and 30 mg/l). Twenty-five fourth instar larvae were subjected to each concentration with three replicates and one control with the same number (n=25) of larvae under optimum laboratory conditions. The larval mortality was calculated after 24 hours. …

Genomic Analysis Of Seven Mycobacteriophages Identifies Three Novel Species With Differing Phenotypic Stabilities, Laura O'Connell, Aidan Coffey Prof., Jim O'Mahony

Department of Biological Sciences Publications

Recently, case studies have been published regarding the application of mycobacteriophage (MP) therapy (MPT) in patients with multi-antibiotic-resistant infections. A major limitation in the development of MPT is the paucity of therapeutically useful MP. As there are approximately 10,000 MP that have yet to be sequenced, it is possible that characterization of this cohort would increase the repertoire of useful MP. This study aims to contribute to such a strategy, by characterizing a cohort of 7 mycobacteriophages. Sequencing analyses revealed that the MP have unique sequences, and subsequent gene annotation revealed differences in gene organization. Notably, MP LOCARD has the …

A Review On Bioremediation Of Heavy Metals And Hydrocarbons Through Plant Growth-Promoting Bacteria And Composting, Hafsa Saleem, Hadia Farooq, Rimsha Mazhar, Sadaf Shakil, Sahar Fazal

Journal of Bioresource Management

Soil is an important ecosystem component; it serves as a habitat for many microscopic organisms that play an important role in the ecosystem's maintenance and crops grown in soil. Unfortunately, human activities have adversely affected not only the environment but also the soil health. Soil has been contaminated with heavy metals hydrocarbons and hydrocarbon-based products that are affecting soil health and the growth of plants. In this review two methods of heavy metal remediation are discussed; one is the role of plant growth-promoting bacteria (PGPB) in the enhancement of phytoremediation and the other is composting. PGPB help plants to grow …

Simultaneous Extraction And Quantitative Analysis Of S-Methyl-L-Cysteine Sulfoxide, Sulforaphane And Glucosinolates In Cruciferous Vegetables By Liquid Chromatography Mass Spectrometry, Armaghan Shafaei, Caroline R. Hill, Jonathan M. Hodgson, Lauren C. Blekkenhorst, Mary C. Boyce

Research outputs 2022 to 2026

Sulfur containing compounds including glucosinolates (GLS), sulforaphane (SFN) and S-methyl-L-cysteine sulfoxide (SMCSO) have been proposed to be partly responsible for the beneficial health effects of cruciferous vegetables. As such, greater understanding of their measurements within foods is important to estimate intake in humans and to inform dietary intervention studies. Herein is described a simple and sensitive method for simultaneous analysis of 20 GLS, SFN and SMCSO by liquid chromatography mass spectrometry. Analytes were effectively retained and resolved on an Xbridge C18 column. Detection can be achieved using high resolution or unit resolution mass spectrometry; the latter making the method more …

Sickle Cell Disease Phenotypes And Obstructive Sleep Apnea; Are They Related?, Suhail Alsaleh, Norah Alshehri, Sara Alsiddiqi, Mohmmed Rayis, Safa Eltahir, Khaled Aldajjam, Mohammed Alzaid, Wadha Alotaibi

Hematology/Oncology and Stem Cell Therapy

ABSTRACT: OBJECTIVE: This study aims to compare the polysomnographic features between Arab-Indian and Benin phenotypes of sickle cell disease (SCD). MATERIALS AND METHODS: This prospective cross-sectional study was conducted in the Children’s Hospital at King Fahad Medical City, in Riyadh where children were recruited from the pediatric hematology clinic and pediatric sleep medicine. All families were approached and patients who met the inclusion criteria and agreed to participate were included in the study. RESULTS: Eighty four children (37 of whom were females) with SCD were included in the study. Their median (interquartile) age was 9 (6.65, 11) years and their …