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2007

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Articles 1 - 30 of 5044

Full-Text Articles in Life Sciences

Hematopoiesis Of Immature Myeloid Dendritic Cells In Stroma-Dependent Spleen Long-Term Cultures Occurs Independently Of Nf-Kb/Relb Function, Jonathan Tan, Keping Ni, Fei Le, Helen O'Neill Sep 2015

Hematopoiesis Of Immature Myeloid Dendritic Cells In Stroma-Dependent Spleen Long-Term Cultures Occurs Independently Of Nf-Kb/Relb Function, Jonathan Tan, Keping Ni, Fei Le, Helen O'Neill

Jonathan Tan

Objective The nuclear factor-κB (NF-KB)/RelB transcription factor plays an essential role in development of some dendritic cell (DC) subsets in mice. In this laboratory, immature myeloid DC are produced in vitro in a stroma-dependent murine spleen long-term culture (LTC) system. In LTC, DC differentiate from hematopoietic progenitors maintained within the stromal cell matrix. Expression and function of RelB in development of LTC-DC has been investigated, with a view to assessing the relationship between DC produced in this system and other known subsets of DC. Materials and Methods RelB expression by LTC-DC was confirmed by detection of protein by Western ...


Concise Review: Dendritic Cell Development In The Context Of The Spleen Microenvironment, Jonathan Tan, Helen O'Neill Sep 2015

Concise Review: Dendritic Cell Development In The Context Of The Spleen Microenvironment, Jonathan Tan, Helen O'Neill

Jonathan Tan

The dendritic cell (DC) population in spleen comprises a mixture of cells including endogenous DC progenitors, DC precursors migrating in from blood and bone marrow, and DC in different states of differentiation and activation. A role for different microenvironments in supporting the dynamic development of murine DC of different types or lineages is considered here. Recent evidence for production of DC dependent on splenic stromal cells is reviewed in the light of evidence that cell production is dependent on cells comprising an endothelial niche in spleen. The possibility that self-renewing progenitors in spleen give rise to DC with tolerogenic or ...


Lysophosphatidic Acid Induces Prostate Cancer Pc3 Cell Migration Via Activation Of Lpa(1), P42 And P38alpha, F Hao, M Tan, Xuemin Xu, J Han, Dd Miller, G Tigyi, Mei-Zhen Cui Jun 2013

Lysophosphatidic Acid Induces Prostate Cancer Pc3 Cell Migration Via Activation Of Lpa(1), P42 And P38alpha, F Hao, M Tan, Xuemin Xu, J Han, Dd Miller, G Tigyi, Mei-Zhen Cui

Mei-Zhen Cui

Prostate cancer cell migration is an essential event both in the progression of prostate cancer and in the steps leading to metastasis. We report here that lysophosphatidic acid (LPA), a potent bioactive phospholipid, induces prostate cancer PC3 cell migration via the activation of the LPA(1) receptor, which is linked to a PTX-sensitive activation mechanism of the mitogen-activated protein kinases (MAPK). Our results demonstrate that parallel activation of ERK1/2 and p38, but not JNK, is responsible for LPA-stimulated PC3 cell migration. Furthermore, using small interfering RNA (siRNA) technology, and overexpressing dominant-negative mutants of p38 MAPK isotypes of alpha, beta ...


A Canine Aflatoxicosis Outbreak And Guidelines For Handling Commercial Dog Food Contaminations, J Smith, Katherine Stenske, Shelley Newman, Claudia Kirk Jun 2013

A Canine Aflatoxicosis Outbreak And Guidelines For Handling Commercial Dog Food Contaminations, J Smith, Katherine Stenske, Shelley Newman, Claudia Kirk

Shelley Newman

No abstract provided.


Detection Of Nitrate/Nitrite Bioavailability In Wastewater Using A Luxcdabebased Klebsiella Oxytoca Bioluminescent Bioreporter, Gary Sayler, Steven Ripp, Desoukky Abd-Elhaleem, Sahar Zaki Jun 2013

Detection Of Nitrate/Nitrite Bioavailability In Wastewater Using A Luxcdabebased Klebsiella Oxytoca Bioluminescent Bioreporter, Gary Sayler, Steven Ripp, Desoukky Abd-Elhaleem, Sahar Zaki

Gary S. Sayler

In the present study, we have constructed a bioluminescent bioreporter for the assessment of nitrate/nitrite bioavailability in wastewater. Specifically, an approximately 500-bp DNA fragment containing a nitrate/nitrite-activated nasR-like promoter (regulating expression of genes encoding nitrite reductase in the genus Klebsiella) was fused upstream of the Vibrio fischeri luxCDABE gene cassette in a modified mini-Tn5 vector. Characterization of this strain, designated W6-1, yielded dose-dependent increased bioluminescence coincident with increased nitrate, nitrite, and ammonium added to the growth medium from 1 to 11 ppm. Bioluminescence in response to nitrogen species addition was light dependent up to 10, 7, and 8 ...


Breast Cancer Cell Proliferation Is Inhibited By Bad: Regulation Of Cyclin D1, R Fernando, Js Foster, A Bible, A Ström, Rg Pestell, M Rao, Arnold Saxton, Seung Baek, K Yamaguchi, Robert Donnell, Maria Cekanova, J Wimalasena Jul 2012

Breast Cancer Cell Proliferation Is Inhibited By Bad: Regulation Of Cyclin D1, R Fernando, Js Foster, A Bible, A Ström, Rg Pestell, M Rao, Arnold Saxton, Seung Baek, K Yamaguchi, Robert Donnell, Maria Cekanova, J Wimalasena

Seung J Baek

Recent investigations suggest that functions of the proapoptotic BCL2 family members, including BAD, are not limited to regulation of apoptosis. Here we demonstrate that BAD inhibits G(1) to S phase transition in MCF7 breast cancer cells independent of apoptosis. BAD overexpression inhibited G(1) transit and cell growth as well as cyclin D1 expression. Inhibition of cyclin D1 expression was mediated through inhibition of transcription activated by AP1. Chromatin immunoprecipitation assays indicated that BAD is localized at the 12-O-tetradecanoylphorbol-13-acetate-response element (TRE) and cAMP-response element (CRE) in the cyclin D1 promoter. This was shown to reflect direct binding interactions of ...


Influence Of Prednisolone On Urinary Calcium Oxalate And Struvite Relative Supersaturation In Healthy Young Adult Female Domestic Shorthaired Cats, N Geyer, Joe Bartges, Claudia Kirk, Sherry Cox, A Hezel, T Moyers, J Hayes Apr 2012

Influence Of Prednisolone On Urinary Calcium Oxalate And Struvite Relative Supersaturation In Healthy Young Adult Female Domestic Shorthaired Cats, N Geyer, Joe Bartges, Claudia Kirk, Sherry Cox, A Hezel, T Moyers, J Hayes

Sherry Cox

Prednisolone (10 mg PO q24h) or placebo was administered to healthy cats for 2 weeks in a masked, placebo-controlled, crossover-design study, and 24-hour urine samples were collected. When cats received prednisolone, 24-hour urine pH was lower and 24-hour urine excretion of creatinine, magnesium, phosphate, and potassium was higher than when cats received placebo. No significant difference was found in urinary relative supersaturation for calcium oxalate (CaOx) or struvite between treatment groups. Prednisolone administration did not induce diuresis, nor was it associated with increased calcium excretion or urinary saturation for CaOx in these healthy cats. Results of this study, however, should ...


Influence Of Hydrochlorothiazide On Urinary Calcium Oxalate Relative Supersaturation In Healthy Young Adult Female Domestic Shorthaired Cat, A Hezel, Joe Bartges, Claudia Kirk, Sherry Cox, N Geyer, T Moyers, J Hayes Apr 2012

Influence Of Hydrochlorothiazide On Urinary Calcium Oxalate Relative Supersaturation In Healthy Young Adult Female Domestic Shorthaired Cat, A Hezel, Joe Bartges, Claudia Kirk, Sherry Cox, N Geyer, T Moyers, J Hayes

Sherry Cox

Hydrochlorothiazide (1 mg/kg PO q12h) or placebo was administered to healthy cats for 2 weeks in a masked, placebo-controlled, crossover-design study, and 24-hour urine samples were collected. When cats received hydrochlorothiazide, 24-hour urine volume, ammonia, chloride, creatinine, magnesium, oxalic acid, phosphate, potassium, and sodium were significantly higher than when cats received placebo. Hydrochlorothiazide was associated with significantly lower urinary saturation for calcium oxalate, but no difference was found in 24-hour urine calcium and citrate, urinary saturation for struvite, or blood ionized calcium. Hydrochlorothiazide decreased urinary saturation for calcium oxalate and could be useful in managing cats with calcium oxalate ...


Idiopathic Systematic Granulomatous Disease, Sandra Sargent, Benjamin Buchanan, Linda Frank, Stephen Kania, David Rotstein, Carla Sommardahl Aug 2011

Idiopathic Systematic Granulomatous Disease, Sandra Sargent, Benjamin Buchanan, Linda Frank, Stephen Kania, David Rotstein, Carla Sommardahl

Carla S. Sommardahl

No abstract provided.


Virtual Microscopy In A Veterinary Curriculum, Michael Sims, S.M. Mendis-Handagama, Robert Moore Jun 2011

Virtual Microscopy In A Veterinary Curriculum, Michael Sims, S.M. Mendis-Handagama, Robert Moore

Michael H. Sims

Teaching faculty in the University of Tennessee College of Veterinary Medicine assist students in their professional education by providing a new way of viewing microscopic slides digitally. Faculty who teach classes in which glass slides are used participate in a program called Virtual Microscopy. Glass slides are digitized using a state-of-the-art integrated system, and a personal computer functions as the "microscope." Additionally, distribution of the interactive images is enhanced because they are available to students online. The digital slide offers equivalent quality and resolution to the original glass slide viewed on a microscope and has several additional advantages over microscopes ...


The Birds Of Nebraska, Revised Edition 2007, Paul A. Johnsgard Dec 2007

The Birds Of Nebraska, Revised Edition 2007, Paul A. Johnsgard

Papers in Ornithology

Persons living in Nebraska often feel that they are living in a cultural wasteland; its citizenry preoccupied with violent sports such as hunting and football. Yet many are unaware that they are actually residing in one of the prime locations in the entire world for observing and enjoying some of the most aesthetically appealing of all the world's biological attractions. The area around Kingsley Dam and Lake McConaughy, for example, is known to have attracted more than 300 bird species, including 104 breeders (plus 17 probable breeders) making it the third-most species-rich bird location in the entire U.S ...


A Guide To The Tallgrass Prairies Of Eastern Nebraska And Adjacent States, Paul A. Johnsgard Dec 2007

A Guide To The Tallgrass Prairies Of Eastern Nebraska And Adjacent States, Paul A. Johnsgard

Papers in Ornithology

Contents

Part 1: Ecology of the Tallgrass Prairie

Part 2: Plants of the Tallgrass Prairies:
Checklist of Prairie & Grassland Plants of Nebraska; Relative Frequencies of Grasses, Spring Creek and Nine-mile Prairies; Twenty Most Abundant Fall Forbs, Spring Creek and Nine-mile Prairies; Typical Shrubs, Forbs, Grasses & Sedges of Eastern Nebraska Tallgrass Prairies; English: Latin Name Equivalents of 370 Tallgrass Prairie Plants; Latin: English Name Equivalents of 370 Tallgrass Prairie Plants Identification Keys to Some Common Nebraska Grassland Forbs

Part 3: Animals of the Tallgrass Prairies
Greater Prairie-chickens and Native Prairies; Seasonal Checklist of Nebraska Tallgrass Ecoregion Birds (Gage County); Tallgrass Prairie Landbirds of Spring Creek & Konza Prairies; Mammals of Nebraska's Eastern Prairies ...


A Guide To The Natural History Of The Central Platte Valley Of Nebraska, Paul A. Johnsgard Dec 2007

A Guide To The Natural History Of The Central Platte Valley Of Nebraska, Paul A. Johnsgard

Papers in Ornithology

This book has been written in the hope that visitors to the Platte Valley may gain a greater appreciation for it through learning some of its animals and plants in addition to the Platte Valley's star spring attraction, its sandhill cranes. Besides the cranes, we have a world-class migration of geese and ducks, a slightly later migration of shorebirds, including what is probably the buff-breasted sandpiper's most important spring staging area between its South American wintering grounds and its arctic breeding grounds. Nebraska also has what may be the largest surviving population of greater prairie-chickens of any state ...


Buffer Projects In The Town Of Wakefield And City Of Somersworth, Julie Labranche Dec 2007

Buffer Projects In The Town Of Wakefield And City Of Somersworth, Julie Labranche

PREP Reports & Publications

Participants in the Wakefield Buffer Project include SRPC staff, members of the Acton Wakefield Watershed Alliance (AWWA), local board and commission members, and the Wakefield Town Planner, Kathy Menici. AWWA members included: Linda Schier, Carol Lafond, Marge Kimball and Adam Soukimas. Town Staff included Joe Fluet (Planning board Chair) and Nancy Spencer Smith (Conservation Commission). The goals of AWWA are to educate and inform shoreland property owners about the harmful effects of erosion and uncontrolled stormwater runoff on water quality of its lakes and ponds. AWWA focuses their on- the-ground efforts to repair, restore and preserve healthy functioning buffers and ...


Seasonal Appearance And Monitoring Of Invasive Species In The Great Bay Estuarine System, Larry G. Harris, Jennifer A. Dijkstra Dec 2007

Seasonal Appearance And Monitoring Of Invasive Species In The Great Bay Estuarine System, Larry G. Harris, Jennifer A. Dijkstra

PREP Reports & Publications

The University of New Hampshire Zoology Department reports on a study designed to synthesize existing data on invasive species in the estuary and the surrounding area, compare succession between two panel studies (1979 to 1982 and 2003 to 2006), seasonally monitor invasive species in the Great Bay Estuary, and identify predators of invasive species. Researchers identified species most likely to invade the Great Bay Estuary, analyzed succession between two long-term panel studies separated by approximately 25 years, collected presence/absence and abundance data of invasive species at four sites within the Great Bay Estuarine System and identified potential predators of ...


Predicting The Success Of Invasive Species In The Great Bay Estuarine Researve, Jennifer A. Dijkstra, Larry G. Harris Dec 2007

Predicting The Success Of Invasive Species In The Great Bay Estuarine Researve, Jennifer A. Dijkstra, Larry G. Harris

PREP Reports & Publications

The University of New Hampshire Zoology Department reports on a study designed to continue monitoring the distribution of invasive species in the Great Bay Estuary and to carry out laboratory experiments designed to test the effects of salinity on ascidian mortality and determine predators of ascidian species. Researchers collected presence/absence and abundance data of invasive species at four sites within the Great Bay Estuarine System. The report gives a brief description of the results of the monitoring program to compare results obtained from 2006 to 2007 and to assess the response of ascidians to varying salinity and predators. This ...


Interagency Science And Research: Quarterly Progress Report, Period Ending December 31, 2007, Margaret N. Rees Dec 2007

Interagency Science And Research: Quarterly Progress Report, Period Ending December 31, 2007, Margaret N. Rees

Interagency Science and Research Strategy

  • Meetings were held with the SNAP Recreation Team and the SNAP Cultural Resources Team to request assistance in the development of the science strategy. Draft versions of interagency goals, subgoals, science questions, and associated tasks were given to each team for review and input.
  • Plans were prepared for the peer review of science proposals submitted for consideration as Conservations Initiatives in SNPLMA Round 9.
  • Proceedings of the natural resources management workshop held on September 13 were completed and sent to the Science & Research Team for review by members.
  • An important focus of the Science and Research Team was the development ...


Lake Mead National Recreation Area Vegetation Monitoring And Management: Quarterly Progress Report, October 1, 2007 To December 31, 2007, Margaret N. Rees Dec 2007

Lake Mead National Recreation Area Vegetation Monitoring And Management: Quarterly Progress Report, October 1, 2007 To December 31, 2007, Margaret N. Rees

Vegetation Monitoring

Executive Summary

  • The Weed Sentry program surveyed more than 750 miles of federal lands in Clark County for invasive, exotic plant species.
  • Weed Sentry also removed more than 600,000 individual invasive plants from federal
    lands. These removals represent pro-active efforts that may have forestalled large
    infestations difficult and costly to eradicate.
  • In response to a request by the manager of the Fish and Wildlife Service Desert National
    Wildlife Refuge, a major effort of surveying springs for plant community composition
    and invasive plants provided unique knowledge services by the Weed Sentry program.
    No such baseline information existed for the Sheep ...


Lake Mead National Recreation Area Sensitive Wildlife Species Monitoring And Analysis: Quarterly Progress Report, Period Ending December 31, 2007, Margaret N. Rees Dec 2007

Lake Mead National Recreation Area Sensitive Wildlife Species Monitoring And Analysis: Quarterly Progress Report, Period Ending December 31, 2007, Margaret N. Rees

Wildlife Monitoring

Project 1. Relict Leopard Frog Monitoring, Management, and Research

  • Fall surveys completed at all natural and translocation sites.
  • Coordination for a potential translocation site on BLM lands in the Gold Butte area ongoing – the aim is that this site will be ready for translocations in 2008.
  • Coordination ongoing for evaluation of a translocation site on BLM lands in the Black Mountains, AZ – the plan is to complete compliance in 2008.
  • Coordination and assistance provided to UNLV research efforts on habitat improvements at Blue Point, Rogers, and Pupfish Refuge springs.
  • Relict Leopard Frog Conservation Team meeting held in December.
  • Draft annual ...


Joint Fire Science Program – Lake Mead National Recreation Area: Revegetating Burned Arid Lands: Identifying Successful Native Species Using Trait And Competition Analysis, Quarterly Progress Report, October 1, 2007 To December 31, 2007, Margaret N. Rees Dec 2007

Joint Fire Science Program – Lake Mead National Recreation Area: Revegetating Burned Arid Lands: Identifying Successful Native Species Using Trait And Competition Analysis, Quarterly Progress Report, October 1, 2007 To December 31, 2007, Margaret N. Rees

Fire Science

The task agreement was awarded to UNLV on October 1, 2007. This report covers the period October 1, 2007 to December 31, 2007. The following activities have been conducted toward meeting or exceeding deliverables in the statement of work: hiring and project start and experimental set-up


Limnological Assistance For The Lake Mead National Recreation Area In Meeting The Challenge Of The Water 2025 Initiative: Quarterly Progress Report, Period Ending: December 1, 2007, Margaret N. Rees Dec 2007

Limnological Assistance For The Lake Mead National Recreation Area In Meeting The Challenge Of The Water 2025 Initiative: Quarterly Progress Report, Period Ending: December 1, 2007, Margaret N. Rees

Limnological Studies

  • The limnologist position has been announced.
  • Project 1: Review of the draft Boulder Basin Adaptive Management Plan is underway.
  • Project 2: Development of a white paper expressing the joint needs of the entities involved in Water 2025 projects is being coordinated.
  • Project 3: A bibliographic reference spreadsheet organizing literature citations related to quagga and zebra mussel research has been completed.
  • Project 4: A comprehensive literature search of Lake Mead limnology is well underway; an Access database has been designed to organize reference citations; and a special collections library at UNLV is being established to house original rare documents and other ...


Pioneering Lobster Aquaculture In Rhode Island, Michael A. Rice Dec 2007

Pioneering Lobster Aquaculture In Rhode Island, Michael A. Rice

Michael A Rice

The 1898 establishment and operation of a lobster hatchery in Wickford Harbor under the auspices of the Rhode Island Commission on Inland Fisheries is reviewed. Dr. Herman Carey Bumpus a professor at Brown University and director of the United States Fisheries Commission undertook this effort to culture lobsters as a result of a decline in lobster landings, and concern for the stocks. Work at the hatchery led do considerable information about lobster reproductive and larval biology, and pioneering aquaculture methods for the species. By the mid-1920s, the hatchery was producing upwards of a million larvae per year and releasing them ...


Regional Body Composition In College-Aged Caucasians From Anthropometric Measures, Robert T. Davidson, Cameron B. Ritchie Dec 2007

Regional Body Composition In College-Aged Caucasians From Anthropometric Measures, Robert T. Davidson, Cameron B. Ritchie

All Faculty Publications

Quantitating fat and lean tissue in isolated body regions may be helpful or required in obesity and health-outcomes research. However, current methods of regional body composition measurement require specialized, expensive equipment such as that used in computed tomography or dual energy x-ray absorptiometry (DEXA). Simple body size or circumference measurement relationships to body composition have been developed but are limited to whole-body applications. We investigated relationships between body size measurements and regional body composition. Methods Using DEXA technology we determined the fat and lean tissue composition for six regions of the body in predominantly Caucasian, college-aged men (n = 32) and ...


Front Matter, Vol. 67 No. 4 Dec 2007

Front Matter, Vol. 67 No. 4

Western North American Naturalist

No abstract provided.


End Matter, Vol. 67 No. 4 Dec 2007

End Matter, Vol. 67 No. 4

Western North American Naturalist

No abstract provided.


An Optical Model For Heat And Salt Budget Estimation For Shallow Seas, Hari Warrior, Kendall L. Carder Dec 2007

An Optical Model For Heat And Salt Budget Estimation For Shallow Seas, Hari Warrior, Kendall L. Carder

Marine Science Faculty Publications

The effects of the underwater light field on heat-budget calculations for shallow waters are developed and applied for the region of Bahamas. Most of the general circulation models use a simplified heat budget scheme based on Jerlov water types, and do not account for optical bottom effects. by optical bottom effect, we mean the bottom absorption and reflection of the short-wave radiation, which in turn affects the thermal stratification and heat exchange with the atmosphere. In this paper, this optical bottom effect is added to a 3D turbulence model (a 1D model called GOTM is coupled to a 3D model ...


A Comparative Study Of Leptospirosis And Dengue In Thai Children, Daniel H. Libraty, Khin S. A. Myint, Clinton K. Murray, Robert V. Gibbons, Mammen P. Mammen, Timothy P. Endy, Wenjun Li, David W. Vaughn, Ananda Nisalak, Siripen Kalayanarooj, Duane R. Hospenthal, Sharone Green, Alan L. Rothman, Francis A. Ennis Dec 2007

A Comparative Study Of Leptospirosis And Dengue In Thai Children, Daniel H. Libraty, Khin S. A. Myint, Clinton K. Murray, Robert V. Gibbons, Mammen P. Mammen, Timothy P. Endy, Wenjun Li, David W. Vaughn, Ananda Nisalak, Siripen Kalayanarooj, Duane R. Hospenthal, Sharone Green, Alan L. Rothman, Francis A. Ennis

Open Access Articles

BACKGROUND: Leptospirosis is an emerging zoonosis that is often under-recognized in children and commonly confused with dengue in tropical settings. An enhanced ability to distinguish leptospirosis from dengue in children would guide clinicians and public health personnel in the appropriate use of limited healthcare resources. METHODOLOGY/PRINCIPAL FINDINGS: We conducted a prospective, hospital-based, study of children with acute febrile illnesses and dengue in Thailand. Among the children without dengue, we identified those with leptospirosis using anti-leptospira IgM and microscopic agglutination titers in paired acute and convalescent blood samples. We then performed a case-control comparison of symptoms, signs, and clinical laboratory ...


Mechanisms Of Ionic Current Changes Underlying Rhythmic Activity Recovery After Decentralization, Olga E. Khorkova Sherman Dec 2007

Mechanisms Of Ionic Current Changes Underlying Rhythmic Activity Recovery After Decentralization, Olga E. Khorkova Sherman

Dissertations

Neuronal networks capableof generating rhythmic output in the absence of patterned sensory or central inputs are widely represented in the nervous system where they support a variety of functions, from learning and memory to rhythmic motor activity such as breathing. To perfectly function in a living organism, rhythm-generating networks have to combine the capability of producing a stable output with the plasticity needed to adapt to the changing demands of the organism and environment. This dissertation used the pyloric network of the crab Cancer borealis to identify potential mechanisms that ensure stability and adaptation of rhythm generation by neuronal networks ...


Effect Of The Co2 Grinding On Modified Atmosphere And Color Shelf Life Of Ground Beef, Sofiane Ghris Dec 2007

Effect Of The Co2 Grinding On Modified Atmosphere And Color Shelf Life Of Ground Beef, Sofiane Ghris

All Theses

Beef ground under air and carbon dioxide (CO2) conditions were evaluated for their storage stability at 2 ± 1¼C starting after 2 days in the dark to simulate transportation, followed by 7 days in lighted display by measuring color (CIE L*, a*, b*, the hue and chroma), gas headspace, microbiological content, and pigment content. AC (ground in air then packaged in 100% CO2 MAP) and CC (ground in CO2 then packaged in 100% CO2 MAP) treatments showed the highest a* and chroma values from day 3 to 9 compared with AV (ground in air then vacuum packaged) and CV (ground in ...


Sara Of Staphylococcus Aureus Binds To The Sara Promoter To Regulate Gene Expression, Ambrose L. Cheung, Koren Nishina, Adhar C. Manna Dec 2007

Sara Of Staphylococcus Aureus Binds To The Sara Promoter To Regulate Gene Expression, Ambrose L. Cheung, Koren Nishina, Adhar C. Manna

Open Dartmouth: Faculty Open Access Articles

The 375-bp sarA open reading frame is driven by three promoters, P1, P3, and P2. Using gel shift and DNase I footprinting assays, we found that SarA binds to two 26-bp sequences and one 31-bp sequence within the P1 and P3 promoters, respectively. Together with the results of transcription analyses, our data indicate that SarA binds to its own promoter to down-regulate sarA expression.