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2004

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Full-Text Articles in Life Sciences

Angiotensin Ii–Induced Protein Kinase D Activation Is Regulated By Protein Kinase C{Delta} And Mediated Via The Angiotensin Ii Type 1 Receptor In Vascular Smooth Muscle Cells, Mingqi Tan, Xuemin Xu, Motoi Ohba, Mei-Zhen Cui Jun 2013

Angiotensin Ii–Induced Protein Kinase D Activation Is Regulated By Protein Kinase C{Delta} And Mediated Via The Angiotensin Ii Type 1 Receptor In Vascular Smooth Muscle Cells, Mingqi Tan, Xuemin Xu, Motoi Ohba, Mei-Zhen Cui

Mei-Zhen Cui

Objective— Angiotensin II (Ang II), through its specific signaling cascades, exerts multiple effects on vascular smooth muscle cells (SMCs). It has been shown that Ang II stimulates activation of protein kinase D (PKD), a member of a new class of serine–threonine kinases. However, little is known regarding the upstream cascade of the intracellular signaling that leads to PKD activation. In the present study, we investigated upstream molecules that mediate Ang II–induced PKD activation in SMCs. Methods and Results— Protein kinase C (PKC) inhibitors completely block Ang II–induced PKD activation, and pretreatment with phorbol 12,13-dibutyrate downregulates Ang ...


Identification Of A New Presenilin-Dependent Zeta-Cleavage Site Within The Transmembrane Domain Of Amyloid Precursor Protein, G Zhao, G Mao, J Tan, Y Dong, Mei-Zhen Cui, Sh Kim, Xuemin Xu Jun 2013

Identification Of A New Presenilin-Dependent Zeta-Cleavage Site Within The Transmembrane Domain Of Amyloid Precursor Protein, G Zhao, G Mao, J Tan, Y Dong, Mei-Zhen Cui, Sh Kim, Xuemin Xu

Mei-Zhen Cui

Gamma-secretase cleavage of beta-amyloid precursor protein (APP) is crucial in the pathogenesis of Alzheimer disease, because it is the decisive step in the formation of the C terminus of beta-amyloid protein (Abeta). To better understand the molecular events involved in gamma-secretase cleavage of APP, in this study we report the identification of a new intracellular long Abeta species containing residues 1-46 (Abeta46), which led to the identification of a novel zeta-cleavage site between the known gamma- and epsilon-cleavage sites within the transmembrane domain of APP. Our data clearly demonstrate that the new zeta-cleavage is a presenilin-dependent event. It is also ...


Natural Resources Outreach Coalition: Coordination And Program Delivery, Stone, A, Frank Mitchell, Amanda Stone Dec 2004

Natural Resources Outreach Coalition: Coordination And Program Delivery, Stone, A, Frank Mitchell, Amanda Stone

PREP Reports & Publications

The Natural Resources Outreach Coalition (NROC) is a multi-organizational initiative providing technical and educational assistance to communities in New Hampshire’s coastal watersheds dealing with the effects of growth. The NROC program offered to communities includes an initial educational public presentation, Dealing with Growth, followed by a series of follow-up meetings to help the community focus their goals, develop an action-oriented work plan, and provide the technical and educational assistance needed to help the community meet its goals.


Wetlands Assessment, Inventory, And Prime Wetlands Designation Project In The Taylor River Watershed, Town Of Hampton, Hampton Conservation Commission, Rockingham County Conservation District Dec 2004

Wetlands Assessment, Inventory, And Prime Wetlands Designation Project In The Taylor River Watershed, Town Of Hampton, Hampton Conservation Commission, Rockingham County Conservation District

PREP Reports & Publications

The purpose of this project was to provide significant educational efforts on wetlands functions and values, prime wetlands designation, on-the-ground inventory findings, wildlife habitat, and New Hampshire's Prime Wetlands laws and rules for the two communities located within the project focus area. The focus area for this project was the Taylor River Watershed, which comprises the Taylor River as it flows through both

Hampton and Hampton Falls, a bit of Ash Brook and Old River as it flows through Hampton, and Grapevine Run as it flows through Hampton Falls. This project assessed over 152 wetland areas containing approximately 620 ...


2004 Support For Shellfish Program And Estuarine Education, Meeker, S & Reid, A, B. Sharon Meeker, Ann S. Reid Dec 2004

2004 Support For Shellfish Program And Estuarine Education, Meeker, S & Reid, A, B. Sharon Meeker, Ann S. Reid

PREP Reports & Publications

Working within the University of New Hampshire (UNH) Cooperative Extension/Sea Grant Program, the Great Bay Coast Watch (GBCW) is New Hampshire’s most wide ranging program for direct citizen involvement in monitoring estuarine and coastal systems. GBCW has a fifteen year history of educating citizens about the Great Bay Estuary, New Hampshire Seacoast, and Gulf of Maine watershed through active participation in monitoring and an accompanying education program. Based in Durham, NH, GBCW coordinates over 100 volunteers drawn from 19 New Hampshire and Southern Maine communities. In 1990, volunteers began monitoring eight sites on a monthly basis April through ...


Dover Natural Resource Mapping And Community Outreach Initiative, Bird, Dover Planning Department Dec 2004

Dover Natural Resource Mapping And Community Outreach Initiative, Bird, Dover Planning Department

PREP Reports & Publications

The grant funding has allowed us to further address the continuing need for community awareness for open space and natural resource protection in the City of Dover. This funding has enabled the City of Dover Planning Department in partnership with the City of Dover Open Lands Committee to present several events throughout October and November 2004. The events offered participants information about natural resource and open space protection as well as an opportunity to enjoy nature. They increased public awareness about the Open Lands Committee and its function. Activities and workshops from October to November 2004 included Apple Harvest Day ...


Modulating Hiv-1 Replication By Rna Interference Directed Against Human Transcription Elongation Factor Spt5, Yueh-Hsin Ping, Chia-Ying Chu, Hong Cao, Jean-Marc Jacque, Mario Stevenson, Tariq M. Rana Dec 2004

Modulating Hiv-1 Replication By Rna Interference Directed Against Human Transcription Elongation Factor Spt5, Yueh-Hsin Ping, Chia-Ying Chu, Hong Cao, Jean-Marc Jacque, Mario Stevenson, Tariq M. Rana

Open Access Articles

BACKGROUND: Several cellular positive and negative elongation factors are involved in regulating RNA polymerase II processivity during transcription elongation in human cells. In recruiting several of these regulatory factors to the 5' long terminal repeat (LTR) promoter during transcription elongation, HIV-1 modulates replication of its genome in a process mediated by the virus-encoded transactivator Tat. One particular cellular regulatory factor, DSIF subunit human SPT5 (hSpt5), has been implicated in both positively and negatively regulating transcriptional elongation but its role in Tat transactivation in vivo and in HIV-1 replication has not been completely elucidated. RESULTS: To understand the in vivo function ...


Systematics Of Dirca (Thymelaeaceae) Based On Its Sequences And Issr Polymorphisms, James A. Schrader, William R. Graves Dec 2004

Systematics Of Dirca (Thymelaeaceae) Based On Its Sequences And Issr Polymorphisms, James A. Schrader, William R. Graves

Horticulture Publications

The genus Dirca consists of three disjunct species of shrubs. Dirca palustris is found in the eastern United States and adjacent Canada; D, occidcntahs is Umited to six counties near the San Francisco Bay in California; and the recently discovered D, mcxicana is known from one isolated population in northeastern Mexico. The three species have been described and classified according to morphological characters, but the morphological evidence does not provide a clear assessment of the relationships among the species. Morphologically D. mexicana most closely resembles D. occidenlalis., but known biogeographical trends raise doubt regarding how the three species are interrelated ...


Regulation Of Cell Cycle By The Anaphase Spindle Midzone, Maki Murata-Hori, Greenfield Sluder, Yu-Li Wang Dec 2004

Regulation Of Cell Cycle By The Anaphase Spindle Midzone, Maki Murata-Hori, Greenfield Sluder, Yu-Li Wang

Open Access Articles

BACKGROUND: A number of proteins accumulate in the spindle midzone and midbody of dividing animal cells. Besides proteins essential for cytokinesis, there are also components essential for interphase functions, suggesting that the spindle midzone and/or midbody may play a role in regulating the following cell cycle.

RESULTS: We microsurgically severed NRK epithelial cells during anaphase or telophase, such that the spindle midzone/midbody was associated with only one of the daughter cells. Time-lapse recording of cells severed during early anaphase indicated that the cell with midzone underwent cytokinesis-like cortical contractions and progressed normally through the interphase, whereas the cell ...


Towards A Systems Approach To Understanding Plant Cell Walls, Chris R. Somerville, Stefan Bauer, Ginger Brininstool, Michelle Facette, Thorsten Hamann, Jennifer Milne, Erin Osborne, Alex Paradez, Staffan Persson, Ted K. Raab, Sonja Vorwerk, Heather Youngs Dec 2004

Towards A Systems Approach To Understanding Plant Cell Walls, Chris R. Somerville, Stefan Bauer, Ginger Brininstool, Michelle Facette, Thorsten Hamann, Jennifer Milne, Erin Osborne, Alex Paradez, Staffan Persson, Ted K. Raab, Sonja Vorwerk, Heather Youngs

Ted K. Raab

One of the defining features of plants is a body plan based on the physical properties of cell walls. Structural analyses of the polysaccharide components, combined with highresolution imaging, have provided the basis for much of the current understanding of cell walls. The application of genetic methods has begun to provide new insights into how walls are made, how they are controlled, and how they function. However, progress in integrating biophysical, developmental, and genetic information into a useful model will require a system-based approach.


The Effect Of Prostaglandin Inhibitor On Pregnancy Rates Of Heifer Embryo Transfer Recipients, Jared William Mcnaughtan Dec 2004

The Effect Of Prostaglandin Inhibitor On Pregnancy Rates Of Heifer Embryo Transfer Recipients, Jared William Mcnaughtan

All Theses and Dissertations

Manipulation of the reproductive tract results in increased levels of prostaglandin, which may, in turn, reduce pregnancy rates in embryo recipients. Administration of a prostaglandin inhibitor prior to embryo transfer improves pregnancy rates in cows. Embryo transfer into heifers is more difficult and often requires additional manipulation of the uterus. This study was designed to determine whether administration of the prostaglandin inhibitor, flunixin meglumine, immediately prior to embryo transfer increases pregnancy rates in heifers. Heifers (n=466) were divided into two equal groups based on BCS (range=6-7) and weight (range=256-455). Estrus was synchronized in heifers by giving two ...


On-Line Resource Clearinghouse For Rapidly Growing Communities, Amy Seif Dec 2004

On-Line Resource Clearinghouse For Rapidly Growing Communities, Amy Seif

PREP Reports & Publications

According to the Environmental Protection Agency, sprawl is among the biggest environmental challenges facing New England, where more than 1,200 acres of open space are lost to development each week. New Hampshire is the fastest growing state in New England, and much of this growth is located within the 42 community coastal watershed served by the New Hampshire Estuaries Project. The Resource Clearinghouse for Rapidly Growing Communities project was created out of an interest in getting community decision makers the information and access to resources that they need to make informed decisions in this challenging time. The clearinghouse is ...


Dietary Isoflavones Alter Regulatory Behaviors, Metabolic Hormones And Neuroendocrine Function In Long-Evans Male Rats, Edwin D. Lephart, James P. Proter, Lihong H. Bu, Trent D. Lund, Kenneth Setchell, Gina Ramoz, William R. Crowley Dec 2004

Dietary Isoflavones Alter Regulatory Behaviors, Metabolic Hormones And Neuroendocrine Function In Long-Evans Male Rats, Edwin D. Lephart, James P. Proter, Lihong H. Bu, Trent D. Lund, Kenneth Setchell, Gina Ramoz, William R. Crowley

All Faculty Publications

Phytoestrogens derived from soy foods (or isoflavones) have received prevalent usage due to their 'health benefits' of decreasing: a) age-related diseases, b) hormone-dependent cancers and c) postmenopausal symptoms. However, little is known about the influence of dietary phytoestrogens on regulatory behaviors, such as food and water intake, metabolic hormones and neuroendocrine parameters. This study examined important hormonal and metabolic health issues by testing the hypotheses that dietary soy-derived isoflavones influence: 1) body weight and adipose deposition, 2) food and water intake, 3) metabolic hormones (i.e., leptin, insulin, T3 and glucose levels), 4) brain neuropeptide Y (NPY) levels, 5) heat ...


Episodic Physical Forcing And The Structure Of Phytoplankton Communities In The Coastal Waters Of New Jersey, Mark A. Moline, Shelley M. Blackwell, Robert Chant, Matthew J. Oliver, Trisha Bergmann, Scott Glenn, Oscar M.E. Schofield Dec 2004

Episodic Physical Forcing And The Structure Of Phytoplankton Communities In The Coastal Waters Of New Jersey, Mark A. Moline, Shelley M. Blackwell, Robert Chant, Matthew J. Oliver, Trisha Bergmann, Scott Glenn, Oscar M.E. Schofield

Biological Sciences

The high variability in physical, biological, and chemical properties in coastal waters have limited our ability to sample the appropriate timescale and space scale to resolve physical forcing of the ecosystem. To improve our understanding, a multiplatform adaptive sampling program at the Long-term Ecosystem Observatory(LEO-15) off the coast of New Jersey examined the relationship between episodic summertime upwelling and downwelling events and the corresponding dynamics in bulk phytoplankton biomass and community structure. Inherent and apparent optical properties were concurrently measured to evaluate the use of optics to improve future sampling coverage in coastal regions. Results indicate peak chlorophyll biomass ...


In Vivo Dose-Response Of Insects To Hz-2v Infection, John P. Burand, Christopher P. Rallis Dec 2004

In Vivo Dose-Response Of Insects To Hz-2v Infection, John P. Burand, Christopher P. Rallis

John Burand

Background Hz-2V infection of female Helicoverpa zea moths is manifested as insects that are either sterile "agonadal" individuals with malformed reproductive tissues or fertile asymptomatic carriers which are capable of transmitting virus on to their progeny. Virus infected progeny arising from eggs laid by asymptomatic carrier females may themselves be either sterile agonadals or asymptomatic carriers. Results By injecting virus into female moths, a correlation was established between virus doses administered to the females and the levels of resulting asymptomatic and sterile progeny. Conclusions The results of these experiments indicate that high virus doses produced a higher level of agonadal ...


In Vivo Dose-Response Of Insects To Hz-2v Infection, John Burand, Christopher Rallis Dec 2004

In Vivo Dose-Response Of Insects To Hz-2v Infection, John Burand, Christopher Rallis

Microbiology Department Faculty Publication Series

Background Hz-2V infection of female Helicoverpa zea moths is manifested as insects that are either sterile "agonadal" individuals with malformed reproductive tissues or fertile asymptomatic carriers which are capable of transmitting virus on to their progeny. Virus infected progeny arising from eggs laid by asymptomatic carrier females may themselves be either sterile agonadals or asymptomatic carriers. Results By injecting virus into female moths, a correlation was established between virus doses administered to the females and the levels of resulting asymptomatic and sterile progeny. Conclusions The results of these experiments indicate that high virus doses produced a higher level of agonadal ...


Behavioral And Physiological Differences In Migratory Strategies Of A Long-Distance Migrant, The Blackpoll Warbler, And A Facultative Short-Distance Migrant, The Yellow-Rumped Warbler, Rebecca L. Holberton Dec 2004

Behavioral And Physiological Differences In Migratory Strategies Of A Long-Distance Migrant, The Blackpoll Warbler, And A Facultative Short-Distance Migrant, The Yellow-Rumped Warbler, Rebecca L. Holberton

University of Maine Office of Research Administration: Grant Reports

The primary focus of these studies has been to investigate the functional role of corticosterone in the development of migratory condition (i.e. hyperphagia, lipogenesis, and migratory restlessness) and to investigate the underlying endocrine mechanisms of migratory condition in two species that differ dramatically in their strategies for reaching their wintering grounds. The main approach has been to measure and to manipulate the peripheral signal molecule, corticosterone, and look at the resulting patterns of feeding behavior, body mass and fat reserves, migratory activity, and metabolites of energy use (fat deposition and protein use). In the comparative component, the Blackpoll warbler ...


Intranasal Vaccination With Streptococcal Fibronectin Binding Protein Sfb1 Fails To Prevent Growth And Dissemination Of Streptococcus Pyogenes In A Murine Skin Infection Model, Jason D. Mcarthur, E. Medina, J. Chin, B. J. Currie, K. S. Sriprakash, S. R. Talay, G. S. Chhatwal, Mark J. Walker Dec 2004

Intranasal Vaccination With Streptococcal Fibronectin Binding Protein Sfb1 Fails To Prevent Growth And Dissemination Of Streptococcus Pyogenes In A Murine Skin Infection Model, Jason D. Mcarthur, E. Medina, J. Chin, B. J. Currie, K. S. Sriprakash, S. R. Talay, G. S. Chhatwal, Mark J. Walker

Faculty of Science - Papers (Archive)

Fibronectin binding protein F1 (Sfb1) of Streptococcus pyogenes (group A streptococcus [GAS]) is a well-characterized adhesin that has been shown to induce protection in mice against a lethal intranasal GAS challenge after intranasal immunization with cholera toxin B subunit (CTB) as adjuvant. With a murine skin infection model, we have shown that Sfb1/CTB vaccination neither elicits opsonizing antibodies nor prevents systemic bacterial growth and dissemination to internal organs after a subcutaneous GAS challenge. These results indicate that an Sfb1-based vaccine should be complemented with additional protective antigens in order to be used in areas such as the tropical north ...


Tsc2 Modulates Actin Cytoskeleton And Focal Adhesion Through Tsc1-Binding Domain And The Rac1 Gtpase, Elena Goncharova, Dmitry Goncharov, Daniel J. Noonan, Vera P Krymskaya Dec 2004

Tsc2 Modulates Actin Cytoskeleton And Focal Adhesion Through Tsc1-Binding Domain And The Rac1 Gtpase, Elena Goncharova, Dmitry Goncharov, Daniel J. Noonan, Vera P Krymskaya

Molecular and Cellular Biochemistry Faculty Publications

Tuberous sclerosis complex (TSC) 1 and TSC2 are thought to be involved in protein translational regulation and cell growth, and loss of their function is a cause of TSC and lymphangioleiomyomatosis (LAM). However, TSC1 also activates Rho and regulates cell adhesion. We found that TSC2 modulates actin dynamics and cell adhesion and the TSC1-binding domain (TSC2-HBD) is essential for this function of TSC2. Expression of TSC2 or TSC2-HBD in TSC2-/- cells promoted Rac1 activation, inhibition of Rho, stress fiber disassembly, and focal adhesion remodeling. The down-regulation of TSC1 with TSC1 siRNA in TSC2-/- cells activated Rac1 and induced loss of ...


2004 Water Quality Education Onboard The Gundalow, Bolster, M, Molly Bolster Dec 2004

2004 Water Quality Education Onboard The Gundalow, Bolster, M, Molly Bolster

PREP Reports & Publications

The Gundalow Company (a non-profit 501(c)3 organization) sponsors maritime history and environmental educational programs for school groups and the public onboard the replica gundalow Captain Edward Adams from May through November in 12-15 riverfront locations in the Piscataqua region. The project funded by the NHEP in 2004 included the development of an expanded Gundalow Company education program curriculum in order to motivate all gundalow visitors to appreciate the relationship between regional maritime history, contemporary water quality issues and future stewardship responsibilities. The NHEP grant also supported the Gundalow Company’s efforts to bring the gundalow to Durham and ...


The Action Component Of Recognition Systems: A Focus On The Response, Aviva E. Liebert, Philip T. Starks Dec 2004

The Action Component Of Recognition Systems: A Focus On The Response, Aviva E. Liebert, Philip T. Starks

Biology Department Faculty Publications

The action component of recognition systems is concerned with the response of an evaluator to perceived cues, based on the dissimilarity of those cues compared with a template. Building upon the historical focus on kin recognition, we apply the framework of conspecific acceptance thresholds to recognition in a broader sense, including interactions within and between organisms, between species, and between living and nonliving things. We review examples from a variety of taxa and recognition contexts to demonstrate when a discriminatory response is expected, and how this response may be flexible depending on the costs and benefits of recognition errors and ...


Registered Dietitans Practicing Advanced Level Skills In The State Of Tennessee And Their Perceived Job Satisfaction., Charlotte Norene Cochran Dec 2004

Registered Dietitans Practicing Advanced Level Skills In The State Of Tennessee And Their Perceived Job Satisfaction., Charlotte Norene Cochran

Electronic Theses and Dissertations

The purpose of this study was to ascertain the number of registered dietitians in Tennessee who perceive they are practicing at advanced levels versus those making recommendations only. Job satisfaction according to order writing privileges was also assessed. A five question survey was sent to hospitals meeting selection criteria. Thirty-three surveys (89%) were returned. Eighty-nine percent of dietitians with order writing privileges considered themselves to be advanced level practitioners compared to 60% in the group of dietitians who did not have order writing privileges. Dietitians with order writing privileges indicated greater job satisfaction compared to dietitians that did not have ...


Conservation Easement Outreach Project, Wenninger, Ray Wenninger Dec 2004

Conservation Easement Outreach Project, Wenninger, Ray Wenninger

PREP Reports & Publications

Stratham voters approved a 5 million dollar conservation bond issue in 2002 to preserve open space through easement or outright purchase. The Ad-hoc Conservation Bond Subcommittee to the Conservation Commission is helping achieve Stratham’s open space goals and is continuing to reach out to landowners that have undeveloped land that may be suitable for Stratham’s open space initiative. It was an opportune time for the subcommittee to provide updated outreach materials when a grant by the Natural Resources Outreach Coalition (NROC) Implementation Grant Program became available. The grant, managed by the New Hampshire Estuaries Project, provided funding to ...


A Comparison Of Linear And Daily Undulating Periodizied Strength Training Programs., Andrew Morrow Caldwell Dec 2004

A Comparison Of Linear And Daily Undulating Periodizied Strength Training Programs., Andrew Morrow Caldwell

Electronic Theses and Dissertations

The purpose of this study is to compare linear periodized (LP) and daily undulating (DUP) periodized strength training programs, and determine if either method of periodization elicits superior gains in 1 Repetitions maximums (1RM) for back squat (BS) and bench press (BP) (core exercises). Nineteen subjects (n=19) underwent a 6-week resistance-training (RT) program. Participants performed both BS and BP two day a week. The LP group linearly increased intensity while decreasing volume. While the DUP group daily changed intensity and volume. Total volume for both BS and BP were equal for both LP and DUP. Both BP and BS ...


An Svd-Based Comparison Of Nine Whole Eukaryotic Genomes Supports A Coelomate Rather Than Ecdysozoan Lineage, Gary W. Stuart, Michael W. Berry Dec 2004

An Svd-Based Comparison Of Nine Whole Eukaryotic Genomes Supports A Coelomate Rather Than Ecdysozoan Lineage, Gary W. Stuart, Michael W. Berry

Faculty Publications and Other Works -- Division of Biology

Background

Eukaryotic whole genome sequences are accumulating at an impressive rate. Effective methods for comparing multiple whole eukaryotic genomes on a large scale are needed. Most attempted solutions involve the production of large scale alignments, and many of these require a high stringency pre-screen for putative orthologs in order to reduce the effective size of the dataset and provide a reasonably high but unknown fraction of correctly aligned homologous sites for comparison. As an alternative, highly efficient methods that do not require the pre-alignment of operationally defined orthologs are also being explored.

Results

A non-alignment method based on the Singular ...


Isolation Of Cancer Stem Cells From Adult Glioblastoma Multiforme, Xianpeng Yuan, James Curtin, Yizhi Xiong, Gentao Liu, Sebastian Waschsmann-Hogiu, Daniel Farkas, Keith Black, John Yu Dec 2004

Isolation Of Cancer Stem Cells From Adult Glioblastoma Multiforme, Xianpeng Yuan, James Curtin, Yizhi Xiong, Gentao Liu, Sebastian Waschsmann-Hogiu, Daniel Farkas, Keith Black, John Yu

Articles

Glioblastoma multiforme (GBM) is the most common adult primary brain tumor and is comprised of a heterogeneous population of cells. It is unclear which cells within the tumor mass are responsible for tumor initiation and maintenance. In this study, we report that brain tumor stem cells can be identified from adult GBMs. These tumor stem cells form neurospheres, possess the capacity for self-renewal, express genes associated with neural stem cells (NSCs), generate daughter cells of different phenotypes from one mother cell, and differentiate into the phenotypically diverse populations of cells similar to those present in the initial GBM. Having a ...


Global Expression Changes Resulting From Loss Of Telomeric Dna In Fission Yeast, Jeffrey G. Mandell, Jürg Bähler, Thomas A. Volpe, Robert A. Martienssen, Thomas R. Cech Dec 2004

Global Expression Changes Resulting From Loss Of Telomeric Dna In Fission Yeast, Jeffrey G. Mandell, Jürg Bähler, Thomas A. Volpe, Robert A. Martienssen, Thomas R. Cech

Chemistry & Biochemistry Faculty Contributions

Background

Schizosaccharomyces pombe cells lacking the catalytic subunit of telomerase (encoded by trt1+) lose telomeric DNA and enter crisis, but rare survivors arise with either circular or linear chromosomes. Survivors with linear chromosomes have normal growth rates and morphology, but those with circular chromosomes have growth defects and are enlarged. We report the global gene-expression response of S. pombe to loss of trt1+.

Results

Survivors with linear chromosomes had expression profiles similar to cells with native telomeres, whereas survivors with circular chromosomes showed continued upregulation of core environmental stress response (CESR) genes. In addition, survivors with circular chromosomes had altered ...


Central Crops And Soils Research Station Highmore, South Dakota: Annual Progress Report, 2004, Agricultural Experiment Station, Plant Science Department Dec 2004

Central Crops And Soils Research Station Highmore, South Dakota: Annual Progress Report, 2004, Agricultural Experiment Station, Plant Science Department

Agricultural Experiment Station and Research Farm Annual Reports

This is the 2004 progress report for the Central Crops and Soils Research Station at Highmore, South Dakota State College. This document represents the research conducted at the Station during the 2004 crop season including: temperature and precipitation data, field evaluations of woody plant materials, optimal management of drought-tolerant legumes and warm season annual grasses, winter wheat breeding, oat research, weed control fertilizer and soil test effects, aphid infestations, barley yellow dwarf Incidence, plant growth and yield of winter wheat in relation to planting date and seed treatment, small grain variety performance trials, corn breeding, and an evaluation of native ...


West River Ag Center Crops And Soils Research Annual Progress Report, 2004, Agricultural Experiment Station Dec 2004

West River Ag Center Crops And Soils Research Annual Progress Report, 2004, Agricultural Experiment Station

Agricultural Experiment Station and Research Farm Annual Reports

This is the 2004 progress report of the West River Crops and Soils Research Projects, South Dakota Agricultural Experiment Station. This document includes reports on: weather and climate, wheat and grain variety trials, management and tillage, and weed and pest control.


Chromosomal Duplications And Cointegrates Generated By The Bacteriophage Lamdba Red System In Escherichia Coli K-12, Anthony R. Poteete, Anita C. Fenton, Ashwini Nadkarni Dec 2004

Chromosomal Duplications And Cointegrates Generated By The Bacteriophage Lamdba Red System In Escherichia Coli K-12, Anthony R. Poteete, Anita C. Fenton, Ashwini Nadkarni

Open Access Articles

BACKGROUND: An Escherichia coli strain in which RecBCD has been genetically replaced by the bacteriophage lambda Red system engages in efficient recombination between its chromosome and linear double-stranded DNA species sharing sequences with the chromosome. Previous studies of this experimental system have focused on a gene replacement-type event, in which a 3.5 kbp dsDNA consisting of the cat gene and flanking lac operon sequences recombines with the E. coli chromosome to generate a chloramphenicol-resistant Lac- recombinant. The dsDNA was delivered into the cell as part of the chromosome of a non-replicating lambda vector, from which it was released by ...