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Full-Text Articles in Life Sciences

Development Of A Rapid Drug Detection Method For Insects Using Paper Spray Ionization Mass Spectrometry (Psi-Ms), Alexandria Plyler May 2021

Development Of A Rapid Drug Detection Method For Insects Using Paper Spray Ionization Mass Spectrometry (Psi-Ms), Alexandria Plyler

Undergraduate Research and Scholarship Symposium

Insects have the ability to reflect the chemical environment that they have developed in. Thus, necrogenous insects can reflect the chemical environment of cadavers they have fed on. Forensic scientists can potentially utilize insect larvae as an alternate biological matrix to detect drugs in cadavers that are skeletonized, burned, or otherwise badly damaged. Analysis of insects using paper spray ionization mass spectrometry (PSI-MS), an analytical method requiring little to no sample preparation, could prove a rapid, cost-effective, and non-destructive alternative form of toxicological analysis. Traditional tissue analysis via GC and LC-MS often require lengthy sample preparation, use expensive reagents, and ...


Nebline, May 2021 May 2021

Nebline, May 2021

The NEBLINE Newsletter Archive from UNL Extension in Lancaster County

Building Health Equity in Lincoln: Extension Launches Health Equity Coalition

Recipe of the Month

Intensive Gardening Techniques

Garden Guide Things to Do This Month

Naturally Occurring Elements in Groundwater--Part 3 of a Series — Fluoride

Cash Rent Survey

Preparing for the Inevitable: Japanese Beetles

Challenge Accepted! Children Thrive Outside

Heart of 4-H Volunteer Award: Rusty & Heidi Hanley

4-H Announcements for 4-H'ers and Volunteers

Clover College: June 15–18, 2021

EXTENSION CALENDAR

4-H’ers Qualify for State Speech & PSA Contest

4-H Horse Stampede Results


Characterizing The Susceptibility And Biological Fitness Of Nebraska Western Corn Rootworm Populations To Pyramided Plant-Incorporated Protectants, Jordan D. Reinders May 2021

Characterizing The Susceptibility And Biological Fitness Of Nebraska Western Corn Rootworm Populations To Pyramided Plant-Incorporated Protectants, Jordan D. Reinders

Dissertations and Student Research in Entomology

The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, is a pest of field corn, Zea mays L., across the United States (U.S.) Corn Belt. Transgenic corn expressing two or more insecticidal proteins (‘pyramid’) derived from Bacillus thuringiensis Berliner (Bt) is a common management strategy employed in areas of continuous corn production. The newest rootworm-Bt pyramid registered in the U.S. is SmartStax® PRO, which contains two rootworm-Bt proteins (Cry3Bb1, Cry34/35Ab1) and DvSnf7 dsRNA. With the pending commercialization of SmartStax® PRO, understanding the current susceptibility of Nebraska WCR populations to the Cry3Bb1 + Cry34/35Ab1 pyramid (SmartStax®) and characterizing ...


Investigating The Role Of Spiders In Integrated Pest Management For Biological Control Of Nebraska Crop Pests, Samantha Daniel May 2021

Investigating The Role Of Spiders In Integrated Pest Management For Biological Control Of Nebraska Crop Pests, Samantha Daniel

Dissertations and Student Research in Entomology

As generalist predators, spiders are capable of fulfilling a wide variety of ecological niches allowing them to intercept and prey upon an array of insect species. This characteristic could be particularly advantageous within agricultural ecosystems. This project sought to reveal the composition and abundance of spider communities within cornfields in western Nebraska and the impact of agronomic practices on those communities as well as determine the potential for spider predation upon two key pests of corn. Spiders were collected from eight corn fields from May to August 2017 and four corn fields from May to August 2018. Additionally, the populations ...


Effect Of A Near-Zero Magnetic Field On Development And Flight Of Oriental Armyworm (Mythimna Separata), Meng-Meng Yan, Lei Zhang, Yun-Xia Cheng, Thomas W. Sappington, Wei-Dong Pan, Xing-Fu Jiang May 2021

Effect Of A Near-Zero Magnetic Field On Development And Flight Of Oriental Armyworm (Mythimna Separata), Meng-Meng Yan, Lei Zhang, Yun-Xia Cheng, Thomas W. Sappington, Wei-Dong Pan, Xing-Fu Jiang

Entomology Publications

The geomagnetic field affects all living organisms on the Earth. In this study we investigated the developmental and behavioral effects of rearing Mythimna separata in a near-zero magnetic field (<500 nT) compared to the local geomagnetic field (approximately 50 µT). The near-zero magnetic field produced by a Helmholtz coil system significantly lengthened larval and pupal development durations, increased male longevity, and reduced pupal weight, female reproduction, and the relative expression level of the vitellogenin (Vg) gene in newly emerged females. Moreover, the near-zero magnetic field had a considerable negative effect on the mating ratio of M. separata adults. In addition, the moths in the near-zero magnetic field displayed less flight activity late in the night than those in the Earth's normal geomagnetic field, indicating that the flight rhythm of M. separata may be affected by the near-zero magnetic field. Reduction in magnetic field intensity may have negative effects on the development and flight of oriental armyworm, with consequent additional effects on its migration.


Design, Simulation And Testing Of Biomimetic Directional Acoustic Sensors, Brendan Francis Apr 2021

Design, Simulation And Testing Of Biomimetic Directional Acoustic Sensors, Brendan Francis

Thinking Matters Symposium

The Ormia ochracea, a species of parasitic fly, has become the focal point in sound localization research because of its finely tuned hearing abilities. The female of this species uses its super highly directional hearing to pinpoint the call of a host cricket, with hypersensitivity of frequency and phase difference, to reach and dispose of its eggs on the host. The goal of this study was to further the research of a previous Project in Professor Guvench’s group which implemented MEMS (Micro Electro-Mechanical System) technology on a chip to replicate these abilities. In this iteration, however, some commercially available ...


Mems Directional Acoustic Sensors, Colby Damren Apr 2021

Mems Directional Acoustic Sensors, Colby Damren

Thinking Matters Symposium

The purpose of my project is to test and verify two Micro-Electro-Mechanical-System (MEMS) microphones. The two MEMS devices are biomimetic microphones that imitate the eardrums of the Ormia Ochracea, a parasitic fly that listens for host crickets to lay their eggs. The MEMS microphones mimic the eardrum spacing of the Ormia Ochracea with a spacing of 1000um. This is roughly twice the opening of the Ormia Ochracea. The microphones will be tested for directionality inside a wooden box. This box will be lined with beveled foam to prevent any echo or outside noise from interfering with the results. These results ...


208— Describing Genetic Diversity In A Non-Native Ant-Mimicking Spider, Cassidy Mills, Jennifer L. Apple Apr 2021

208— Describing Genetic Diversity In A Non-Native Ant-Mimicking Spider, Cassidy Mills, Jennifer L. Apple

GREAT Day

The ant-mimicking spider Myrmarachne formicaria (Salticidae) is a species native to Eurasia and was first identified in North America in 2001. It has since been found in many locations in the Northeast including western New York, western Pennsylvania, northeastern Ohio, and southern Ontario. Little is known about its introduction to North America and how it has dispersed since. By characterizing the mitochondrial genetic diversity of this species, we can learn about its introduction history and dispersal patterns in North America. Sequencing of a 600-bp mitochondrial DNA gene region spanning the 16s rRNA, leucine tRNA, and part of the ND1 gene ...


Piojos En Los Humanos, Erin W. Hodgson, Celina Wille Apr 2021

Piojos En Los Humanos, Erin W. Hodgson, Celina Wille

All Current Publications

Las infestaciones de piojos en humanos, llamadas pediculosis, son muy comunes en todo el mundo. Los piojos pueden transmitir enfermedades y son insectos parásitos en humanos, mascotas y ganado. Los piojos pertenecen al orden Phthiraptera y generalmente se dividen en dos grupos según las piezas bucales y las preferencias alimentarias. Los piojos masticadores (Mallophaga) tienen mandíbulas bien desarrolladas y comen piel, plumas y pelaje de aves y mamíferos.


Key Factors Involved In Reduction Of Damage To Sunflower By The European Sunflower Moth In China Through Late Planting, Yunxia Cheng, Thomas W. Sappington, Lizhi Luo, Chenguang Liu, Yongjun Wang, Shuangping Liu, Zongze Zhang, Lijun Wang, Xingfu Jiang Apr 2021

Key Factors Involved In Reduction Of Damage To Sunflower By The European Sunflower Moth In China Through Late Planting, Yunxia Cheng, Thomas W. Sappington, Lizhi Luo, Chenguang Liu, Yongjun Wang, Shuangping Liu, Zongze Zhang, Lijun Wang, Xingfu Jiang

Entomology Publications

The European sunflower moth, Homoesoma nebulellum (Denis et Schiffermüller), emerged as a major new pest in Bayannur, China, in 2006. Insecticidal control with a single application is problematic because timing is critical, and multiple applications increase production and environmental costs. Management of H. nebulellum by planting date adjustment can be effective, but the optimal time window for late planting is unknown. Natural levels of H. nebulellum infestation were compared among sunflowers planted on five dates from April 25 to June 5 in two years, and the relationship between timing of adult abundance and flowering assessed. Delaying planting of sunflower from ...


Insects Provide Unique Systems To Investigate How Early-Life Experience Alters The Brain And Behavior, Rebecca R. Westwick, Clare C. Rittschof Apr 2021

Insects Provide Unique Systems To Investigate How Early-Life Experience Alters The Brain And Behavior, Rebecca R. Westwick, Clare C. Rittschof

Entomology Faculty Publications

Early-life experiences have strong and long-lasting consequences for behavior in a surprising diversity of animals. Determining which environmental inputs cause behavioral change, how this information becomes neurobiologically encoded, and the functional consequences of these changes remain fundamental puzzles relevant to diverse fields from evolutionary biology to the health sciences. Here we explore how insects provide unique opportunities for comparative study of developmental behavioral plasticity. Insects have sophisticated behavior and cognitive abilities, and they are frequently studied in their natural environments, which provides an ecological and adaptive perspective that is often more limited in lab-based vertebrate models. A range of cues ...


Chinche De La Semilla Del Olmo, Ryan Davis, Celina Wille Apr 2021

Chinche De La Semilla Del Olmo, Ryan Davis, Celina Wille

All Current Publications

En julio del 2014, el Laboratorio de Diagnóstico de Plagas de Plantas de Utah y USDA APHIS confirmaron el primer reporte de chinche de semilla del olmo (Lygaeidae: Arocatus melanocephalus) en el estado de Utah. Originaria de Europa, la chinche de la semilla del olmo se identificó por primera vez en los Estados Unidos en Idaho en 2012. Desde entonces se ha encontrado en Oregón, Washington, Columbia Británica, Canadá y en varios lugares de Asia y Europa. Si bien el primer reporte en Utah se originó en el condado de Salt Lake, la chinche de la semilla del olmo ahora ...


Surveys Of Bees At Midewin National Tallgrass Prairie With Special Focus On The Possible Presence Of A Rusty Patched Bumble Bee Populations, Nicole Dede Apr 2021

Surveys Of Bees At Midewin National Tallgrass Prairie With Special Focus On The Possible Presence Of A Rusty Patched Bumble Bee Populations, Nicole Dede

Scholar Week 2016 - present

Native bees are one of the most diverse and important groups of pollinators in many ecosystems. However, pollinators are declining around the world and bumble bees (Bombus sp.) have been significantly affected. Prairie grasslands like those at Midewin National Tallgrass Prairie (Wilmington, IL) are likely prime locations for many species of bees due to rich and consistent floral resources. An important example is the rusty patched bumble bee, B. affinis, which was found at Midewin in 2018. B. affinis is federally listed as a critically endangered species that has experienced a 90% range reduction since 2000. This study is meant ...


Correlation Between Abundance Of Fossils In Harvester Ant Nest And Proximity Of Fossil Sites, Hayeong Woo Apr 2021

Correlation Between Abundance Of Fossils In Harvester Ant Nest And Proximity Of Fossil Sites, Hayeong Woo

Campus Research Day

Harvester ants have an interesting behavior of bone collecting. Therefore, harvester ant nests that are positioned around the fossil sites contain various fossil fragments. If there is a statistically significant correlation between the abundance of fossil fragments and the distance of the ant nests from the main fossil sites, ant nests can potentially be used to predict the proximity of the main fossil sites.


High Tunnel Pest Management - Aphids, Nick Volesky, Zachery R. Schrumm Apr 2021

High Tunnel Pest Management - Aphids, Nick Volesky, Zachery R. Schrumm

All Current Publications

Aphids are a common pest found on high tunnel crops such as fruits, vegetables, ornamentals, grasses, and weeds. Four aphid species commonly found in Utah in high tunnels are green peach aphid (Myzus persicae), melon aphid (Aphis gossypii), potato aphid (Macrosiphum euphorbiae), and cabbage aphid (Brevicoryne brassicae). This fact sheet describes different aphids and reviews the aphid life cycle. It also addresses how to monitor for damage caused by aphids and cultural, biological, and chemical controls.


What’S The Buzz?, Christine L. Moskalik Apr 2021

What’S The Buzz?, Christine L. Moskalik

Teacher Resources

No abstract provided.


Blue Orchard Bee (Osmia Lignaria): Commercial Pollinator For Orchards, Theresa Pitts-Singer Mar 2021

Blue Orchard Bee (Osmia Lignaria): Commercial Pollinator For Orchards, Theresa Pitts-Singer

All Current Publications

The most common mason bee in Utah is the blue orchard bee. Blue orchard bees collect pollen from a large variety of flowering plants but prefer fruit trees from the family Rosaceae. Recent concerns about honey bees, coupled with the blue orchard bee’s superior pollination of tree fruits, have increased interest in rearing blue orchard bees.


Two New Species Of Palm-Leaf Skeletonizers (Lepidoptera: Pterolonchidae: Homaledra Busck), James E. Hayden Mar 2021

Two New Species Of Palm-Leaf Skeletonizers (Lepidoptera: Pterolonchidae: Homaledra Busck), James E. Hayden

Insecta Mundi

Two species of palm-leaf skeletonizers (Lepidoptera: Pterolonchidae: Homaledra Busck) that are invasive in Florida, USA are newly described. Homaledra howardi new species is described from Florida and the Dominican Republic. Homaledra knudsoni new species is described from Florida, southern Texas, and Mexico. A lectotype is designated for Homaledra sabalella (Chambers). The three are diagnosed and com­pared with other congeners, and Homaledra is compared with other Pterolonchidae. The larvae of the three common species feed on epidermis of palm foliage, causing significant damage.

Se describen dos especies de palomillas de hojas de palma (Lepidoptera: Pterolonchidae: Homale­dra Busck) que son ...


A Catalogue Of The Cybocephalidae (Coleoptera: Cucujoidea) Of The World, Trevor Randall Smith Mar 2021

A Catalogue Of The Cybocephalidae (Coleoptera: Cucujoidea) Of The World, Trevor Randall Smith

Insecta Mundi

A world catalogue and bibliography of the family Cybocephalidae (Coleoptera) is presented. The catalogue contains 207 species within 16 genera. Each taxon is accompanied by a complete taxonomic his­tory, including a full annotated synonymy with original references cited. The reversal of the recent synonymy of Cybocephalus brevis Grouvelle over Cybocephalus minimus Grouvelle is proposed. Cybocephalus aeneus Reiche is removed from synonymy with Cybocephalus micans Reitter and considered incertae sedis.

The Cybocephalidae are a family of small predatory beetles found throughout the world (Smith 2020). While cybocephalids are typically extremely convex, shiny and dark in coloration, the defining characteristic of ...


The Strange Case Of Colophotia Miranda Olivier, 1886 (Coleoptera: Lampyridae: Luciolinae), Lesley Ballantyne, Wan F.A. Jusoh Mar 2021

The Strange Case Of Colophotia Miranda Olivier, 1886 (Coleoptera: Lampyridae: Luciolinae), Lesley Ballantyne, Wan F.A. Jusoh

Insecta Mundi

A single male specimen identified as Colophotia miranda Olivier (Coleoptera: Lampyridae: Lucio­linae) by Blair and housed in the Natural History Museum, London, is redescribed, and its identification and affinities explored.

Ernest Olivier, who first described Colophotia miranda in 1886, was then curator of insects at the Muséum natio­nale d’Histoire naturelle, Paris (MNHN). Effectively for lampyridologists he was the first in a narrow line of firefly taxonomists. Thanks to Olivier we have our first subfamily classifications, keys, catalogues and species descrip­tions; work that has undergone much revision and expansion in the last 100 years.

One of Olivier ...


Subsequent Spelling Ritchiei For Mnematium Ritchii Macleay, 1821 (Coleoptera: Scarabaeoidea: Scarabaeidae: Scarabaeini) To Be Maintained For Prevailing Usage, Stefano Ziani Mar 2021

Subsequent Spelling Ritchiei For Mnematium Ritchii Macleay, 1821 (Coleoptera: Scarabaeoidea: Scarabaeidae: Scarabaeini) To Be Maintained For Prevailing Usage, Stefano Ziani

Insecta Mundi

The specific name in Mnematium ritchiei Macleay, 1821 (Coleoptera: Scarabaeidae: Scarabaeini) is an incorrect subsequent spelling—the original spelling is ritchii—but, because of its prevailing usage here documented, is to be preserved.


Electrophysiological And Behavioral Responses Of An Ambrosia Beetle To Volatiles Of Its Nutritional Fungal Symbiont, Christopher M. Ranger, Marek Dzurenko, Jenny Barnett, Ruchika Geedi, Louela Castrillo, Matthew Ethington, Matthew Ginzel, Karla Addesso, Michael E. Reding Mar 2021

Electrophysiological And Behavioral Responses Of An Ambrosia Beetle To Volatiles Of Its Nutritional Fungal Symbiont, Christopher M. Ranger, Marek Dzurenko, Jenny Barnett, Ruchika Geedi, Louela Castrillo, Matthew Ethington, Matthew Ginzel, Karla Addesso, Michael E. Reding

Agricultural and Environmental Sciences Faculty Research

Ambrosia beetles (Coleoptera: Scolytinae) cultivate their fungal symbiont within host substrates as the sole source of nutrition on which the larvae and adults must feed. To investigate a possible role for semiochemicals in this interaction, we characterized electrophysiological and behavioral responses of Xylosandrus germanus to volatiles associated with its fungal symbiont Ambrosiella grosmanniae. During still-air walking bioassays, X. germanus exhibited an arrestment response to volatiles of A. grosmanniae, but not antagonistic fungi Beauveria bassiana, Metarhizium brunneum, Trichoderma harzianum, the plant pathogen Fusarium proliferatum, or malt extract agar. Solid phase microextraction-gas chromatography-mass spectrometry identified 2-ethyl-1-hexanol, 2-phenylethanol, methyl benzoate and 3-methyl-1-butanol in ...


Protura (Arthropoda: Hexapoda) In Slovenian Caves, Loris Galli, Franc Janžekovič, Peter Kozel, Tone Novak Mar 2021

Protura (Arthropoda: Hexapoda) In Slovenian Caves, Loris Galli, Franc Janžekovič, Peter Kozel, Tone Novak

International Journal of Speleology

Protura is a small class of Hexapoda, generally poorly known, and rather scarce data on its occurrence in caves are scattered throughout literature on fauna in caves and in some papers on Protura. Although the cave-dwelling fauna is relatively well studied in Slovenia, published records on Protura are rare. In this paper, data on the occurrence and abundance of Protura in Slovenian caves are considered. Various statistical analyses were performed to compare caves with Protura to those without in 60 intensively monitored cavities to detect any differences in the selected environmental conditions. No significant difference was obtained. Samples collected from ...


Microhabitat Selection Of A Sicilian Subterranean Woodlouse And Its Implications For Cave Management, Giuseppe Nicolosi, Stefano Mammola, Salvatore Costanzo, Giorgio Sabella, Rosolino Cirrincione, Giovanni Signorello, Marco Isaia Mar 2021

Microhabitat Selection Of A Sicilian Subterranean Woodlouse And Its Implications For Cave Management, Giuseppe Nicolosi, Stefano Mammola, Salvatore Costanzo, Giorgio Sabella, Rosolino Cirrincione, Giovanni Signorello, Marco Isaia

International Journal of Speleology

Human activities in subterranean environments can affect different ecosystem components, including the resident fauna. Subterranean terrestrial invertebrates are particularly sensitive to environmental change, especially microclimatic variations. For instance, microclimate modifications caused by the visitors may directly affect local fauna in caves opened to the public. However, since numerous factors act synergistically in modulating the distribution and abundance of subterranean species, it remains challenging to differentiate the impact of human intervention from that of other factors. Therefore, evidence of the impact of tourism on cave invertebrate fauna remains scarce. Over a year and with approximately two visits a month, we investigated ...


The Importance Of Time And Place: Nutrient Composition And Utilization Of Seasonal Pollens By European Honey Bees (Apis Mellifera L.), Gloria Degrandi-Hoffman, Vanessa Corby-Harris, Mark Carroll, Amy L. Toth, Stephanie Gage, Emily Watkins Dejong, Henry Graham, Mona Chambers, Charlotte Meador, Bethany Obernesser Mar 2021

The Importance Of Time And Place: Nutrient Composition And Utilization Of Seasonal Pollens By European Honey Bees (Apis Mellifera L.), Gloria Degrandi-Hoffman, Vanessa Corby-Harris, Mark Carroll, Amy L. Toth, Stephanie Gage, Emily Watkins Dejong, Henry Graham, Mona Chambers, Charlotte Meador, Bethany Obernesser

Entomology Publications

Honey bee colonies have a yearly cycle that is supported nutritionally by the seasonal progression of flowering plants. In the spring, colonies grow by rearing brood, but in the fall, brood rearing declines in preparation for overwintering. Depending on where colonies are located, the yearly cycle can differ especially in overwintering activities. In temperate climates of Europe and North America, colonies reduce or end brood rearing in the fall while in warmer climates bees can rear brood and forage throughout the year. To test the hypothesis that nutrients available in seasonal pollens and honey bee responses to them can differ ...


Changes In Both Trans- And Cis-Regulatory Elements Mediate Insecticide Resistance In A Lepidopteron Pest, Spodoptera Exigua, Bo Hu, He Huang, Songzhu Hu, Miaomiao Ren, Qi Wei, Xiangrui Tian, Mohammed Esmail Abdalla Elzaki, Chris Bass, Jianya Su, Subba Reddy Palli Mar 2021

Changes In Both Trans- And Cis-Regulatory Elements Mediate Insecticide Resistance In A Lepidopteron Pest, Spodoptera Exigua, Bo Hu, He Huang, Songzhu Hu, Miaomiao Ren, Qi Wei, Xiangrui Tian, Mohammed Esmail Abdalla Elzaki, Chris Bass, Jianya Su, Subba Reddy Palli

Entomology Faculty Publications

The evolution of insect resistance to insecticides is frequently associated with overexpression of one or more cytochrome P450 enzyme genes. Although overexpression of CYP450 genes is a well-known mechanism of insecticide resistance, the underlying regulatory mechanisms are poorly understood. Here we uncovered the mechanisms of overexpression of the P450 gene, CYP321A8 in a major pest insect, Spodoptera exigua that is resistant to multiple insecticides. CYP321A8 confers resistance to organophosphate (chlorpyrifos) and pyrethroid (cypermethrin and deltamethrin) insecticides in this insect. Constitutive upregulation of transcription factors CncC/Maf are partially responsible for upregulated expression of CYP321A8 in the resistant strain. Reporter gene ...


Squash Bug Integrated Pest Management, Nick Volesky, Mair Murray Mar 2021

Squash Bug Integrated Pest Management, Nick Volesky, Mair Murray

All Current Publications

This infographic reviews integrated pest management for squash bugs. It includes information on the following: resistant varieties of vegetables to plant and crop rotation, homemade traps, mechanical destruction, mulches, trap crops, natural enemies, and organic and synthetic insecticides.


Spatio-Temporal Selection Of Reference Genes In The Two Congeneric Species Of Glycyrrhiza, Yuping Li, Xiaoju Liang, Xuguo Zhou, Yu An, Ming Li, Ling Yuan, Yongqing Li, Ying Wang Mar 2021

Spatio-Temporal Selection Of Reference Genes In The Two Congeneric Species Of Glycyrrhiza, Yuping Li, Xiaoju Liang, Xuguo Zhou, Yu An, Ming Li, Ling Yuan, Yongqing Li, Ying Wang

Entomology Faculty Publications

Glycyrrhiza, a genus of perennial medicinal herbs, has been traditionally used to treat human diseases, including respiratory disorders. Functional analysis of genes involved in the synthesis, accumulation, and degradation of bioactive compounds in these medicinal plants requires accurate measurement of their expression profiles. Reverse transcription quantitative real-time PCR (RT-qPCR) is a primary tool, which requires stably expressed reference genes to serve as the internal references to normalize the target gene expression. In this study, the stability of 14 candidate reference genes from the two congeneric species G. uralensis and G. inflata, including ACT, CAC, CYP, DNAJ, DREB, EF1, RAN, TIF1 ...


Nebline, March 2021 Mar 2021

Nebline, March 2021

The NEBLINE Newsletter Archive from UNL Extension in Lancaster County

Naturally Occurring Elements in Groundwater - Part 1 of a Series — Calcium and Magnesium

Celebrate National Nutrition Month

RECIPE OF THE MONTH

2021 All-America Selections Vegetable Winners

Starting Seeds Indoors

Garden Guide Things to Do This Month

Planning for Spring Calving Season

Chemigation Trainings

2021 Nebraska On-Farm Research Annual Results Update Meetings

Houseplants: Simple Solutions to Plant Pests

Heart of 4-H Volunteer Award: Whitney Lehn

Meet 2020–2021 4-H Council

4-H Announcements for 4-H'ers and Volunteers

Private Pesticide Applicator Trainings by Zoom

EXTENSION CALENDAR

Upcoming Early Childhood Trainings


De Novo Whole Genome Assembly Of The Swede Midge (Contarinia Nasturtii), A Specialist Of Brassicaceae, Using Linked-Read Sequencing, Boyd A. Mori, Cathy Coutu, Yolanda H. Chen, Erin O. Campbell, Julian R. Dupuis, Martin A. Erlandson, Dwayne D. Hegedus Feb 2021

De Novo Whole Genome Assembly Of The Swede Midge (Contarinia Nasturtii), A Specialist Of Brassicaceae, Using Linked-Read Sequencing, Boyd A. Mori, Cathy Coutu, Yolanda H. Chen, Erin O. Campbell, Julian R. Dupuis, Martin A. Erlandson, Dwayne D. Hegedus

Entomology Faculty Publications

The swede midge, Contarinia nasturtii, is a cecidomyiid fly that feeds specifically on plants within the Brassicaceae. Plants in this family employ a glucosinolate-myrosinase defense system, which can be highly toxic to non-specialist feeders. Feeding by C. nasturtii larvae induces gall formation, which can cause substantial yield losses thus making it a significant agricultural pest. A lack of genomic resources, in particular a reference genome, has limited deciphering the mechanisms underlying glucosinolate tolerance in C. nasturtii, which is of particular importance for managing this species. Here, we present an annotated, scaffolded reference genome of C. nasturtii using linked-read sequencing from ...