Life Sciences Commons^™

Adapting Cell-Free Protein Synthesis As A Platform Technology For Education, Grace W. Chu, Max Z. Levine, Nicole E. Gregorio, Javin P. Oza

STAR (STEM Teacher and Researcher) Presentations

Cell-free protein synthesis (CFPS) has emerged as an enabling biotechnology for research and biomanufacturing as it allows for the production of protein without the need for a living cell. Applications of CFPS include the construction of libraries for functional genomics and structural biology, the production of personalized medicine, and the expression of virus-like particles. The absence of a cell wall provides an open platform for direct manipulation of the reaction conditions and biological machinery. This project focuses on adapting the CFPS biotechnology to the classroom, making a hands-on bioengineering approach to learning protein synthesis accessible to students grades K-16 through ...

The Cal Poly Basic Needs Task Force Report - Fall 2018, Aydin Nazmi

Food Science and Nutrition

No abstract provided.

Comparison Of An Endangered Species’ Seed Bank Before And After Ungulate Removal, Melanie K. Phillips, Kathryn Mceachern

STAR (STEM Teacher and Researcher) Presentations

The purpose of this study was to assess the impact of the removal of ungulates on the soil seed bank of the endemic Santa Rosa Island manzanita (Arctostaphylos confertiflora). A. confertiflora was trampled and browsed by non-native ungulates between the years 1850 and 2011 which reduced its ability to produce seeds.This species is an obligate seeder, meaning that sprouting from seeds in the soil is its only method of reproduction. Due in part to the lack of its soil seed bank, this species was federally listed as endangered by the US Fish and Wildlife Service in 1997. In order ...

Microfluidic Electrical Impedance Spectroscopy, John J. Foley

Master's Theses and Project Reports

The goal of this study is to design and manufacture a microfluidic device capable of measuring changes in impedance valuesof microfluidic cell cultures. Tocharacterize this, an interdigitated array of electrodes was patterned over glass, where it was then bonded to a series of fluidic networks created in PDMS via soft lithography. The device measured ethanol impedance initially to show that values remain consistent over time. Impedance values of water and 1% wt. saltwater were compared to show that the device is able to detect changes in impedance, with up to a 60% reduction in electrical impedance in saltwater. Cells were ...

Comparative Analysis Of Body Composition And Reference Values Of Visceral Adipose Tissues In Various American Collegiate Sports, Eshan M. Dandekar

Master's Theses and Project Reports

Background: Currently, body composition (BC) assessment is usually performed to diagnose disease states and accurately estimate certain types of tissues. In athletes, performing BC assessments helps gauge training and nutrition programs to see if they are adequately meeting the athlete’s needs to improve performance. Annual Dual Energy X-ray Absorptiometry (DXA) scans before the start of an athlete’s season can help identify an athlete’s preparedness or health before training begins.

Objective: To assess the preseason BC of four collegiate sports: Men’s basketball, men’s baseball, women’s volleyball, and women’s soccer, for Fat Mass (FM), bone ...

An Assessment Of The Greatest Impacts On Distribution Uniformity For Drip And Micro Irrigation, Brooke Ashley Worden

Master's Theses and Project Reports

Using the Cal Poly Irrigation Training and Research Centers (ITRC) drip/micro evaluation program, global, or system, DU_lq is computed by combining the component DU_lq values of: pressure variation, uneven spacing between emitters, unequal drainage and “other” causes. “Other” causes include plugging, wear and manufacturing coefficient. The program also computes what percentage of the non-uniformity is due to each component. Burt (2004) showed that over 95% of the non-uniformity is due to “Other” causes and pressure differences. This thesis looks at what specifically in those components is driving the non-uniformity by analyzing various equipment and field practices and ...

Influence Of A Guanidine Riboswitch In Bacterial Cells, Erik S. Timsak, Jefferey A. Kimbrel, Chris S. Ward, Rhona K. Stuart, Michael P. Thelen

STAR (STEM Teacher and Researcher) Presentations

Bacteria live in environments containing complex ecologies of other microbes that communicate and survive through the action of a variety of small metabolic compounds. One common yet relatively unstudied metabolite is guanidine. Although it can be toxic to cells, recent studies have revealed that guanidine may function as a cellular metabolite through a specialized RNA sequence known as a riboswitch. Within our overall project on improving algal productivity, the focus of this study is to: (a) describe various guanidine riboswitch sequences in bacteria that interact with biofuel producing algae; and (b) determine if guanidine has a positive or negative influence ...

Tof-Sims Analysis Of Plant Seed Interactions With Plant Growth-Promoting Bacteria, Tammy Pheuphong, Rachel Komorek, Xiao-Ying Yu

STAR (STEM Teacher and Researcher) Presentations

The use of time-of-flight secondary ion mass spectrometry (ToF-SIMS) in plant biology is a relatively unexplored and quickly developing field. The majority of research in plant SIMS involves the application of ToF-SIMS to study dried wood tissues, and only a handful of studies apply SIMS on plant stems, roots, and/or seeds. Our project provides a brief description and review of previous work using SIMS on plant stems, roots, and/or seeds, along with an emphasis on the sample preparation in each study. Additionally, the use of Brachypodium distachyon (Brachypodium) as a model system for research on grasses has also ...

Impacts Of Nutrient Enrichment On Native And Non-Native Plants In Serpentine Soil, Amber R. Williams, Mary Devlin, N. Rajakaruna

STAR (STEM Teacher and Researcher) Presentations

Serpentine soil, derived from serpentinite, California’s state rock, is characterized by its harsh nature - due to its low levels of essential nutrients and exceptionally high levels of toxic heavy metals.In California, serpentine soils are characterized by high levels of plant endemism and rarity. Plants occurring in serpentine soil exhibit extreme tolerance of harsh soil conditions, and are typically out-competed in ‘normal’ soils due to the cost associated with physiological specialization. Atmospheric nutrient deposition is contributing to a greater invasion of non-native grass species on serpentine soils, outcompeting the native species and threatening them with local extinction. Our greenhouse ...

Scale-Invariant Geometric Data Analysis (Sigda), Marina Girgis, Max Robinson

STAR (STEM Teacher and Researcher) Presentations

The purpose of this research is to introduce a new data analysis method called Scale Invariant Geometric Data Analysis (SIGDA). SIGDA has been shown to be more informative than more common data analysis methods, such as Principal Component Analysis (PCA). SIGDA is used to visualize complex data sets in a way that accurately preserves data patterns and behavior. SIGDA is designed to preserve relative ratios in a numerical matrix, and the number of entries has to be more than the total number of rows and columns. Our research involved providing a simple explanation of SIGDA's mathematical process—simple enough ...

Modifying The Mineral Profile Of Crickets (Acheta Domesticus) Using A Supplemented Diet, Rhianne Morgan Le Maxwell

Master's Theses and Project Reports

Captive insectivores may consume invertebrates as all, or part of their overall diet. The challenge with feeding captive insectivores involves the limited number of invertebrate species that are commercially available, and the lack of key nutrients provided by these insects. Among these insects, a naturally occurring low concentration of calcium and an inverse calcium to phosphorus ratio may put insectivores at the risk of developing hypocalcemia. A strategy to correct this nutrient imbalance involves supplementing the insect diet with high concentrations of targeted nutrients – a term referred to as gut-loading. Current industry guidelines recommend feeding a supplemented diet for 48 ...

Detecting A Probiotic Product Within The Gut Of Broiler Chickens, Anneka Pisula

Master's Theses and Project Reports

As of January 2017, the U.S. poultry industry banned the use of antibiotics and now relies on alternatives such as probiotics to help protect animal health. Although probiotic use is not a new concept in the poultry industry, identifying the best combination of bacterial strains to generate an effective probiotic formula requires further investigation. This study aimed to detect a probiotic product of four bacterial strains (Pedioccoccus acidilactici, Pediococcus pentosaceus, Lactobacillus plantarum, and Bacillus subtilis) in a feeding trial with broiler chickens. Birds given the probiotic were predicted to show an improved growth performance with the probiotics colonizing the ...

Assessment Of Granulovirus, Spinosad, And Mating Disruption For Controlling Cydia Pomonella L. [Lepidoptera: Tortricidae] In Organic Coastal California Apple Orchards, Raven Lukehart

Master's Theses and Project Reports

Codling moth, Cydia pomonella[Lepidoptera: Tortricidae], is a major entomological pest of apples, pears, and walnuts cross the world (Pajac et al. 2016). Female codling moths lay eggs on the apple exocarp and larvae burrow within the fruit causing economic losses to fruit growers.Organic apple orchards in San Luis Obispo, CA currently have three codling moth, Cydia pomonella,control options commercially available including granulovirus (CpVG), spinosad, and mating disruption. In field tests on apple (Malus), we compare percent fruit injury between treatments of granulovirus (2.43 oz/ha Cyd-X® organically approved, Certis USA, Columbia MD), spinosad (4.05 oz ...

Evaluation Of Endothelial Cell Responses To Elevated Glucose, Gabriella Sugerman

Master's Theses and Project Reports

Developing a tissue-engineered Blood Vessel Mimic (BVM) to represent diabetic macrovascular disease could expedite design of new vascular devices specifically tailored to diabetic patients. In contribution toward this model, this thesis assessed Human Umbilical Vein Endothelial Cell (HUVEC) responses to high glucose conditions. Interleukin 6 (IL-6) and Cluster of Differentiation 36 (CD36) were selected to signify oxidative stress activity, a hallmark of diabetic macrovascular disease. Next, activity of potential reference genes B2M, HPRT1, and ACTB was assessed. All genes were found to exceed acceptable variability, so the E-ΔC T method of data analysis was selected. Next, cellular responses to high ...

Crossing Corridors: Wildlife Use Of Jumpouts And Undercrossings Along A Highway With Wildlife Exclusion Fencing, Alex J. Jensen, Alex Joseph Jensen

Master's Theses and Project Reports

Roads pose two central problems for wildlife: wildlife-vehicle collisions (WVCs) and habitat fragmentation. Wildlife exclusion fencing can reduce WVCs but can exacerbate fragmentation. In Chapter 1, I summarize the relevant studies addressing these two problems, with a focus on large mammals in North America. Chapters 2 and 3 summarize field assessments of technologies to reduce WVCs and maintain connectivity, specifically jumpout ramps and underpasses, along Highway 101 near San Luis Obispo, CA. In a fenced highway, some animals inevitably breach the fence and become trapped, which increases the risk of a wildlife-vehicle collision. Earthen escape ramps, or “jumpouts”, can allow ...

The Effect Of Destoning And Enzymatic Pretreatments On The Biofuel Production From Olive Cake, Patrick Tai

Master's Theses and Project Reports

More than 16,000 tons of olive cake was produced in the United States in 2017. Olive cake is a by-product of olive oil extraction, which has limited animal feed potential, and poses an environmental threat when landfilled due to its high organic load and polyphenol content. This residue has potential for biofuel (bioethanol and biomethane) production because it is rich in polysaccharides such as pectin, hemicellulose, and cellulose. Yet, olive cake contains olive stones that can impede its conversion to biofuel. Therefore, two methods of destoning, centrifugation and screening by horizontal screw press, were first compared. Both methods removed ...

The Future Of Strawberry Production, Audrey Rebecca Ponce

Agricultural Education and Communication

California’s agriculture industry is greatly diverse, with strawberries as a sector that most native Californians wouldn’t recognize as a large contributor to California’s agricultural economy. Within the last couple of years, strawberry production has undergone massive changes that are rapidly modifying the way strawberries are cultivated. The future of strawberry production will have completely new methods for growing, fumigating, and harvesting in just five to ten years (Guthman, 2016). The Cal Poly Strawberry Center opened in 2014 with a mission to “increase the sustainability of the California strawberry industry through research and education that addresses industry needs ...

Preparation And Characterization Of Eva/Cort Microparticles For Rattlesnake Endocrinology Studies, Alexander C. Pauer

Materials Engineering

No abstract provided.

Hosting The Third Annual Young Women In Agriculture Career And Leadership Seminar, Emma E. Morris, Madyline J. Braught

Agricultural Education and Communication

Agriculture has traditionally been a male-dominated industry; however, recent years have shown a significant growth in female presence. Women are beginning to take leadership roles both on the farm and within advocacy, lobbying, and business entities in the industry. According to the latest agricultural census data from the United States Department of Agriculture (USDA), in 2012, women farmers controlled seven percent of the nation’s farmland (Census, 2014). Additionally, women held leadership roles in 14 percent of the nation’s two million farms (Census, 2014). This data is several years old, and likely the numbers have grown since the last ...

Soil Science In Precision Agriculture Lesson Plan, Carson Ellis Cronkright

Agricultural Education and Communication

Due to the increasing demands for the American agricultural industry to provide exceptional products at an increasing rate of production, as well as implement the practice of sustainable farming, it is imperative that more modern concepts be taught at a younger age (Ray, 2013). In order to inform students of advancements and the future of agriculture in the United States, curriculum and lesson plans should reflect this movement in industry in order to provide relevant information and maintain the highest quality of education possible. This project will provide a constructed lesson plan as one example of adopting more progressive methods ...

Comparative Analysis Of Physiological Measurements And Environmental Metrics On Predicting Heat Stress Related Events, Mckenzie Lee Barlow

Master's Theses and Project Reports

Exposure to high heat and humidity can lead to serious health risks, including heat exhaustion and heat stroke. Wet Bulb Globe Temperature (WBGT) and heat index have historically been used to predict heat stress events, but individualized factors are not included in the measurement. It has been shown that there is a relationship between cardiovascular measurements and heat stress, which could be used to measure heat stress risk on an individual level. Research has been done to find relationships between cardiovascular metrics in a workplace environment, however the study did not include the use of a controlled environment as a ...

Bird Abundance At Bird Feeders In Response To Temperature, Wind Speed And Precipitation During The Winter Season, Siddhant Kahal

Master's Theses and Project Reports

The goal of this project is to explore how 23 different bird species respond to 3 climatic attributes. These attributes are lower than average temperatures, wind speed and precipitation level. Information about the bird species and all of the data associated with them is provided by Project FeederWatch (PFW). This is a citizen based survey study that provides key information about bird species abundance through the use of backyard and community feeders. The study volunteers from across the United States and Canada monitor these bird feeders and note important information about the species such as the number of individuals seen ...

Effect Of Bioaugmentation Product Biowish® Aqua™ On Nitrogen Removal In Wastewater, Patrick Cassidy Kalvass

Master's Theses and Project Reports

Biological nutrient removal (BNR) from wastewater, and specifically nitrogen removal, is a growing concern to wastewater dischargers such as municipalities. Excess nutrients in effluent can create problems such as eutrophication, toxicity to aquatic life, and dissolved oxygen depletion in receiving waters. BNR systems have been installed in many locations with success, but their operation presents operational and financial demands greater than conventional biological treatment. Nitrogen removal is typically performed in sequential autotrophic nitrification and denitrification, which increases needed energy input, operational complexity, and therefore cost. Simultaneous nitrification-denitrification (SNdN) achieved in a single system has also been successfully implemented, however operational ...

Pink And Dude Chefs: Effectiveness Of An After-School Nutrition Knowledge And Culinary Skills Program For Middle School Students To Increase Fruit And Vegetable Consumption, Alyssa S. Vaziri

Master's Theses and Project Reports

The rate of overweight and obesity among adolescents aged 12-19 years has more than tripled since 1980, and disproportionately impacts low-income and marginalized populations. Reduction in adolescent obesity rates may result in decreased health risks, decrease healthcare costs, and increased quality of life. Effective intervention methods for adolescent participants have incorporated nutrition knowledge and culinary skill building into afterschool programs. This study examines whether building knowledge, skills, and confidence through a culinary intervention can improve adolescent participants’ choices of healthful foods through increased fruit and vegetable intake.

Pink and Dude Chefs (PDC) is an afterschool nutrition education and culinary skills ...

Effect Of Aquaponic Vs. Hydroponic Nutrient Solution, Led Light Intensity And Photoperiod On Indoor Plant Growth Of Butterhead, Romaine And Kale (L. Sativa, B. Oleracea), Sean M. Foster

Master's Theses and Project Reports

Vertical farming has been proposed as a solution for providing food security for an increasing, urbanized human population. Light-emitting diode (LED) technology has become increasingly affordable and efficient, making it an ideal choice as artificial lighting for indoor farms. Still largely undiscovered parameters are the optimal plant varieties and types of production systems for plant growth, profit, and human nutrition. Aquaponics may be able to provide sustainable animal protein for vertical farms, increasing their ability to provide more substantial nutrition to consumers. This research aimed to better understand vertical farming as a food production system, and to determine if aquaponics ...

Farmers For America Documentary Screening, Tiffany Jane Thompson

Agricultural Education and Communication

The agriculture industry is seeing a decrease in the number of beginning farms, operations that have been in existence for 10 years or less, across the United States. According to the most current Census of Agriculture by the United States Department of Agriculture (USDA), beginning farms make up approximately a quarter of the 2.1 million total farms across the country. This is a 20 percent drop between the 2007 and the 2012 censuses (2012 Census Highlights, Beginning Farmers, 2017). At the same time, the average age of the U.S. farmer is increasing and is now at 58 (Williamson ...

Malene Wines: The Beginning Of A Brand, Tyler Menane

Agricultural Education and Communication

Malene Wines is the most recently developing brand under the Crimson Wine Group. Currently, the brand is being produced and managed under Chamisal Vineyards. Malene Wines started off with one brand of rosé wine, that has only been available for purchase in the Chamisal tasting room or wholesale. This has caused confusion for the consumer in differentiating the two brands. To help begin the separation of the two brands, Malene Wines has recently received a modified Airstream trailer to serve as a mobile tasting room.

Whereas Chamisal Vineyards is a brand focused on Chardonnay and Pinot Noir with small production ...

Cal Poly Farmers Picnic, Emily Anne Dale, Riley Nilsen, Jack Gavron

Agricultural Education and Communication

Agriculture can be seen as the world’s most essential industry. Many products people use daily come directly from agriculture, including the food placed on people’s tables. Knowing where that food comes from, and the overall importance of agricultural education is vital in the United States and the world. This message needs to be shared beyond the classroom: we need agriculture to survive.

According to the United States Department of Agriculture, “in 2012, there were 3.2 million farmers, ranchers and other agricultural managers and an estimated 757,900 agricultural workers legally employed in the U.S.” (USDA, 2012 ...

Cal Poly's Western Bonanza Junior Livestock Show Hosts First Annual Livestock Auction: The Best In The West Livestock Auction, Karl W. Montague, Tatum E. Holdener

Agricultural Education and Communication

Through this senior project, the authors researched whether or not Cal Poly's Western Bonanza Junior Livestock Show's first ever livestock auction was viable. Through their research, they found the addition of a livestock show to be successful and something that should continue.

Safety Videos For Major Shop Equipment In Cal Poly Brae Lab 1, Trey Joseph Johnston

Agricultural Education and Communication

At California Polytechnic State University, San Luis Obispo, students from all majors can take a variety of classes in the BioResource and Agriculture Engineering Department (BRAE). It is essential for future high school mechanics educators to learn the basic shop tools and the importance of safety in the laboratory. In shop 1 of the BRAE Department, there are many different tools students will find in a mechanics classroom. The five main power tools are the Table Saw, Drill Press, Bench Grinder, Radial Arm Saw, and Disk/Belt Sander.

In BRAE 121 Agricultural Mechanics, students learn basic shop skills about lab ...