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Full-Text Articles in Life Sciences

An Orange Fluorescent Protein Tagging System For Real-Time Pollen Tracking, J. Rice, R. Mundell, R. Millwood, O. Chambers, L. Abercrombie, H. Davies, C. Stewart Dec 2102

An Orange Fluorescent Protein Tagging System For Real-Time Pollen Tracking, J. Rice, R. Mundell, R. Millwood, O. Chambers, L. Abercrombie, H. Davies, C. Stewart

C. Neal Stewart Jr.

Background Monitoring gene flow could be important for future transgenic crops, such as those producing plant-made-pharmaceuticals (PMPs) in open field production. A Nicotiana hybrid (Nicotiana. tabacum × Nicotiana glauca) shows limited male fertility and could be used as a bioconfined PMP platform. Effective assessment of gene flow from these plants is augmented with methods that utilize fluorescent proteins for transgenic pollen identification. Results We report the generation of a pollen tagging system utilizing an orange fluorescent protein to monitor pollen flow and as a visual assessment of transgene zygosity of the parent plant. This system was created to generate a tagged ...


Untangling The Economic And Social Impediments To Producer Adoption Of Organic Wheat, Donya L. Ralph-Quarnstrom May 2019

Untangling The Economic And Social Impediments To Producer Adoption Of Organic Wheat, Donya L. Ralph-Quarnstrom

All Graduate Theses and Dissertations

Consumer demand for organic products has shown double-digit growth in recent years encouraging the development of a wider range of goods (Greene, 2017). Americans with an annual household income under $30,000 actively purchase organic foods at nearly the same rate as households with over $75,000 in annual incomes, 42% versus 49% (Greene et al., 2017). Previous research observed the adoption of organic farming practices on a combination of different grains, fruits and vegetables, meat, and dairy products from across the globe. However, this is the first study to examine the adoption of organic wheat in the Western U ...


Exterranean [Table Of Contents], Phillip John Usher Mar 2019

Exterranean [Table Of Contents], Phillip John Usher

Literature

“For anyone who might be suffering from Anthropocene fatigue, this is a book to jolt you from your slumbers. What happens to the globe when we shift attention from the outward projection of emissions to extraction ? The Earth we thought we knew, and were already mourning, takes on a stunning new critical light.”—Claire Colebrook, Pennsylvania State University

Exterranean concerns the extraction of stuff from the Earth, a process in which matter goes from being sub- to exterranean. By bringing a rich archive of nonmodern texts and images from across Europe, into conversation with the contemporary work of Bruno Latour ...


Stability Analysis Of Kernel Quality Traits In Exotic-Derived Doubled Haploid Maize Lines, Adam Vanous, Candice Gardner, Michael Blanco, Adam Martin-Schwarze, Jinyu Wang, Xianran Li, Alexander E. Lipka, Sherry Flint-Garcia, Martin Bohn, Jode Edwards, Thomas Lubberstedt Mar 2019

Stability Analysis Of Kernel Quality Traits In Exotic-Derived Doubled Haploid Maize Lines, Adam Vanous, Candice Gardner, Michael Blanco, Adam Martin-Schwarze, Jinyu Wang, Xianran Li, Alexander E. Lipka, Sherry Flint-Garcia, Martin Bohn, Jode Edwards, Thomas Lubberstedt

Agronomy Publications

Variation in kernel composition across maize (Zea mays L.) germplasm is affected by a combination of the plant’s genotype, the environment in which it is grown, and the interaction between these two elements. Adapting exotic germplasm to the US Corn Belt is highly dependent on the plant’s genotype, the environment where it is grown, and the interaction between these components. Phenotypic plasticity is ill-defined when specific exotic germplasm is moved over large latitudinal distances and for the adapted variants being created. Reduced plasticity (or stability) is desired for the adapted variants, as it allows for a more rapid ...


Farmer Attitudes Toward Cooperative Approaches To Herbicide Resistance Management: A Common Pool Ecosystem Service Challenge, David E. Ervin, Elise H. Breshears, George B. Frisvold, Terrance M. Hurley, Katherine E. Dentzman, Jeffrey L. Gunsolus, Raymond A. Jussaume, Micheal D. K. Owen, Jason Norsworthy, Mustofa Mahmud Al Mamun, Wesley Everman Mar 2019

Farmer Attitudes Toward Cooperative Approaches To Herbicide Resistance Management: A Common Pool Ecosystem Service Challenge, David E. Ervin, Elise H. Breshears, George B. Frisvold, Terrance M. Hurley, Katherine E. Dentzman, Jeffrey L. Gunsolus, Raymond A. Jussaume, Micheal D. K. Owen, Jason Norsworthy, Mustofa Mahmud Al Mamun, Wesley Everman

Environmental Science and Management Faculty Publications and Presentations

Dramatic growth in herbicide-resistant (HR) weeds in the United States threatens farm profitability and may undercut environmentally beneficial farming practices. When HR weeds move across farm boundaries due to ecological processes or human action, a common pool resource challenge emerges, requiring farmer cooperation to manage such weeds effectively. We investigate the scope for cooperative management using responses to a national survey on HR weed issues to test a recursive model of three preconditions for collective action: (1) concern about HR weeds migrating from nearby lands; (2) communication with neighbors about HR weeds; and (3) belief that cooperation is necessary for ...


Wildfire Activity And Land Use Drove 20th‐Century Changes In Forest Cover In The Colorado Front Range, Kyle Rodman, Thomas T. Veblen, Sara Saraceni, Teresa B. Chapman Feb 2019

Wildfire Activity And Land Use Drove 20th‐Century Changes In Forest Cover In The Colorado Front Range, Kyle Rodman, Thomas T. Veblen, Sara Saraceni, Teresa B. Chapman

Geography Graduate Contributions

Recent shifts in global forest area highlight the importance of understanding the causes and consequences of forest change. To examine the influence of several potential drivers of forest cover change, we used supervised classifications of historical (1938–1940) and contemporary (2015) aerial imagery covering a 2932‐km2study area in the northern Front Range (NFR) of Colorado and we linked observed changes in forest cover with abiotic factors, land use, and fire history. Forest cover in the NFR demonstrated broad‐scale changes 1938–2015 and overall cover increased 7.8%, but there was notable spatial variability and many sites also experienced ...


Interacting Stressors Matter: Diet Quality And Virus Infection In Honeybee Health, Adam G. Dolezal, Jimena Carrillo-Tripp, Timothy M. Judd, W. Allen Miller, Bryony C. Bonning, Amy L. Toth Feb 2019

Interacting Stressors Matter: Diet Quality And Virus Infection In Honeybee Health, Adam G. Dolezal, Jimena Carrillo-Tripp, Timothy M. Judd, W. Allen Miller, Bryony C. Bonning, Amy L. Toth

Ecology, Evolution and Organismal Biology Publications

Honeybee population declines have been linked to multiple stressors, including reduced diet diversity and increased exposure to understudied viral pathogens. Despite interest in these factors, few experimental studies have explored the interaction between diet diversity and viral infection in honeybees. Here, we used a mixture of laboratory cage and small semi-field nucleus hive experiments to determine how these factors interact. In laboratory experiments, we found that high-quality diets (polyfloral pollen and high-quality single-source pollen) have the potential to reduce mortality in the face of infection with Israeli acute paralysis virus (IAPV). There was a significant interaction between diet and virus ...


Heterodera Glycines Utilizes Promiscuous Spliced Leaders And Demonstrates A Unique Preference For A Species-Specifc Spliced Leader Over C. Elegans Sl1, Stacey N. Barnes, Rick E. Masonbrink, Tom R. Maier, Arun S. Seetharam, Anoop S. Sindhu, Andrew J. Severin, Thomas J. Baum Feb 2019

Heterodera Glycines Utilizes Promiscuous Spliced Leaders And Demonstrates A Unique Preference For A Species-Specifc Spliced Leader Over C. Elegans Sl1, Stacey N. Barnes, Rick E. Masonbrink, Tom R. Maier, Arun S. Seetharam, Anoop S. Sindhu, Andrew J. Severin, Thomas J. Baum

Office of Biotechnology Publications

Spliced leader trans-splicing (SLTS) plays a part in the maturation of pre-mRNAs in select species across multiple phyla but is particularly prevalent in Nematoda. The role of spliced leaders (SL) within the cell is unclear and an accurate assessment of SL occurrence within an organism is possible only after extensive sequencing data are available, which is not currently the case for many nematode species. SL discovery is further complicated by an absence of SL sequences from high-throughput sequencing results due to incomplete sequencing of the 5'-ends of transcripts during RNA-seq library preparation, known as 5'-bias. Existing datasets and ...


Forage News [2019-02], University Of Kentucky Department Of Plant And Soil Sciences Feb 2019

Visualizing Relative Potential For Aquatic Ecosystem Toxicity Using The Epa Toxics Release Inventory And Life Cycle Assessment Methods, Theodore Langlois, Michael Carbajales-Dale, Elizabeth Carraway Jan 2019

Visualizing Relative Potential For Aquatic Ecosystem Toxicity Using The Epa Toxics Release Inventory And Life Cycle Assessment Methods, Theodore Langlois, Michael Carbajales-Dale, Elizabeth Carraway

Journal of South Carolina Water Resources

The U.S. EPA Toxic Release Inventory has been available since 1987 as a record of industrial releases of toxic chemicals following the 1986 Emergency Planning and Community Right-to-Know Act. Combining this release data with estimates of relative toxicity of these chemicals to aquatic systems increases the value of the database by providing a common basis for comparison. The Tool for Reduction and Assessment of Chemicals and Other Environmental Impacts is a database of characterization factors to assess environmental impacts. It was used to develop relative ecotoxicity impacts and interpreted using Life Cycle Assessment concepts. The visualization software Tableau was ...


Understanding Greenhouse Gases, Julie M. Mueller, Laura Edwards, Madhav P. Nepal, Larry B. Browning, Matthew L. Miller, P. Troy White Jan 2019

Understanding Greenhouse Gases, Julie M. Mueller, Laura Edwards, Madhav P. Nepal, Larry B. Browning, Matthew L. Miller, P. Troy White

iLEARN Curriculum Resources

Students will conduct hands-on experiments to see how greenhouse gases interact with the Earth’s atmosphere and how greenhouse gases affect temperature. This lesson introduces National Geographic’s Geo-Inquiry Process, where students will identify a Geo-inquiry question, collect data, and create a project around the answer to their question. Students will then present their findings to their peers and evaluate their Geo-Inquiry process.


Grazing Myths That Reduce Profitability, Greg S. Halich Jan 2019

Grazing Myths That Reduce Profitability, Greg S. Halich

Forage Symposium at the Kentucky Cattlemen’s Convention

Many cattle farms have moved to rotational grazing in the past decade, and there are significant benefits associated with this grazing technique. However, one thing that I have noticed is that costs are not always accounted for when it comes to recommended rotational grazing practices. If you ignore costs, partially or fully, you will invariably do too much of whatever you are considering. In this light, I will highlight four grazing recommendations (myths) that many folks are often taking to extremes related to rotational grazing, and one additional myth (generic to grazing style) that is reducing the overall profitability of ...


Economics Of Grazing Cover Crops, Ed Ballard Jan 2019

Economics Of Grazing Cover Crops, Ed Ballard

Forage Symposium at the Kentucky Cattlemen’s Convention

Feed costs represent the major cost in most livestock production systems. A completed analysis of 225 Standardized Performance Analysis (SPA) Beef Cow Records on herds in Illinois and Iowa showed that feed cost was the overriding factor determining profitability, explaining over 57 percent of the herd-to-herd variation.


Profitability At Eldon Farms: Guiding Principles, John Genho Jan 2019

Profitability At Eldon Farms: Guiding Principles, John Genho

Forage Symposium at the Kentucky Cattlemen’s Convention

Eldon Farms is a 7,000-acre farm in the Northern Piedmont of Virginia. Over the past 15 years, we have adopted a grazing program based on three principles. While the specifics of each operation are different, I believe these principles can be applied across a host of situations.


2018 Long-Term Summary Of Kentucky Forage Variety Trials, Gene L. Olson, S. Ray Smith, Jimmy C. Henning Jan 2019

2018 Long-Term Summary Of Kentucky Forage Variety Trials, Gene L. Olson, S. Ray Smith, Jimmy C. Henning

Forage Symposium at the Kentucky Cattlemen’s Convention

Forage crops occupy approximately 7 million acres in Kentucky. Forages provide a majority of the nutrition for beef, dairy, horse, goat, sheep, and wildlife in the state. In addition, forage crops play an environmentally friendly role in soil conservation, water quality, and air quality. There are over 60 forage species adapted to the climate and soil conditions of Kentucky. Only 10 to 12 of these species occupy the majority of the acreage, but within these species there is a tremendous variation in varieties.

This publication was developed to provide a user-friendly guide to choosing the best variety for producers based ...


Forages At Kca Foreword [2019], Christopher D. Teutsch, Christi L. Forsythe, Rehanon Pampell Jan 2019

Forages At Kca Foreword [2019], Christopher D. Teutsch, Christi L. Forsythe, Rehanon Pampell

Forage Symposium at the Kentucky Cattlemen’s Convention

No abstract provided.


Influence Of Nitrogen And Sprig Planting Rates On Surface Runoff Losses During 'Latitude 36' Bermudagrass Establishment, Lance A. Rice Jan 2019

Influence Of Nitrogen And Sprig Planting Rates On Surface Runoff Losses During 'Latitude 36' Bermudagrass Establishment, Lance A. Rice

LSU Master's Theses

The hybrid bermudagrass (Cynodon dactylon x C. transvaalensis) ‘Latitude 36’ is grown because of its quality, excellent wear tolerance and fast recuperation. Hybrid bermudagrasses are vegetatively propagated as sprigs with N applied to accelerate establishment. Since losses of soil and associated nutrients in runoff are relatively high when ground coverage is low, rapid establishment is desirable from an environmental besides aesthetic basis. However, neither the interactive effect of sprig density and N fertilization on the rate of bermudagrass expansion nor soil and nutrient loss during establishment has been carefully examined. The current work measured bermudagrass coverage, runoff volume and concentrations ...


Lingnan Gardeners Newsletter (No. 41) = 嶺南彩園通訊 (第41期), Lingnan Gardeners, Kwan Fong Cultural Research And Development Programme, Lingnan University Jan 2019

Lingnan Gardeners Newsletter (No. 41) = 嶺南彩園通訊 (第41期), Lingnan Gardeners, Kwan Fong Cultural Research And Development Programme, Lingnan University

Lingnan Gardeners Newsletter 嶺南彩園通訊

Pomegranate 石榴花


A Botanist's Take On The Fruits Of The Spirit, Jeff Ploegstra Jan 2019

A Botanist's Take On The Fruits Of The Spirit, Jeff Ploegstra

Faculty Work Comprehensive List

"Love, joy, peace, patience, kindness, goodness, faithfulness, gentleness, and self-control are all signs of a vital and thriving relationship with Christ, but at the heart of these fruits lies a seed."

Posting about living out Christ-like virtues from In All Things - an online journal for critical reflection on faith, culture, art, and every ordinary-yet-graced square inch of God’s creation.

https://inallthings.org/a-botanists-take-on-the-fruits-of-the-spirit/


Forage Quality Of Cool Season Perennial Grass Horse Pastures In The Transition Zone, Annmarie Christine Riley Jan 2019

Forage Quality Of Cool Season Perennial Grass Horse Pastures In The Transition Zone, Annmarie Christine Riley

Theses and Dissertations--Plant and Soil Sciences

Cool season perennial grasses are the foundation of equine nutrition in the transition zone. The objective of this study was to evaluate forage quality using ADF, NDF, IVTDMD, CP, WSC, and ESC and changes in vegetative swards seasonally, diurnally, across species (Kentucky bluegrass, tall fescue, orchardgrass, and perennial ryegrass) and cultivar. This study was conducted in 2015 and 2017 and plots were maintained vegetatively with two to four week mowing. Morning and afternoon sample collection occurred monthly during the growing season. Samples were flash frozen; freeze dried, ground, and scanned using Near Infrared Reflectance Spectroscopy (NIRS) to predict forage quality ...


Effects Of Harvest Time On The Aroma Of White Wines Made From Cold-Hardy Brianna And Frontenac Gris Grapes Using Headspace Solid-Phase Microextraction And Gas Chromatography-Mass Spectrometry-Olfactometry, Somchai Rice, Madina Tursumbayeva, Matthew Clark, David Greenlee, Murlidhar Dharmadhikari, Anne Fennell, Jacek A. Koziel Jan 2019

Effects Of Harvest Time On The Aroma Of White Wines Made From Cold-Hardy Brianna And Frontenac Gris Grapes Using Headspace Solid-Phase Microextraction And Gas Chromatography-Mass Spectrometry-Olfactometry, Somchai Rice, Madina Tursumbayeva, Matthew Clark, David Greenlee, Murlidhar Dharmadhikari, Anne Fennell, Jacek A. Koziel

Agricultural and Biosystems Engineering Publications

The Midwest wine industry has shown a marked increase in growers, hectares planted, wineries, and wine production. This growth coincides with the release of cold-hardy cultivars such as Brianna and Frontenac gris, in 2001 and 2003, respectively. These white grape varieties account for one-third of the total area grown in the state of Iowa. It is generally accepted that the wine aroma profile plays a crucial role in developing a local, sustainable brand. However, the identity of Brianna/Frontenac Gris-based wine aromas and their link to the grape berry chemistry at harvest is unknown. This study aims to preliminarily characterize ...


Arabidopsis Bioinformatics Resources: The Current State, Challenges, And Priorities For The Future, Colleen Doherty, Justin Walley, Eve Wurtele, Et Al. Jan 2019

Arabidopsis Bioinformatics Resources: The Current State, Challenges, And Priorities For The Future, Colleen Doherty, Justin Walley, Eve Wurtele, Et Al.

Genetics, Development and Cell Biology Publications

Effective research, education, and outreach efforts by the Arabidopsis thalianacommunity, as well as other scientific communities that depend on Arabidopsis resources, depend vitally on easily available and publicly‐shared resources. These resources include reference genome sequence data and an ever‐increasing number of diverse data sets and data types. TAIR (The Arabidopsis Information Resource) and Araport (originally named the Arabidopsis Information Portal) are community informatics resources that provide tools, data, and applications to the more than 30,000 researchers worldwide that use in their work either Arabidopsis as a primary system of study or data derived from Arabidopsis. Four ...


Strain-Specific Protein Interaction And Localization Of Two Strains Of Potato Yellow Dwarf Virus And Functional Domains Of Their Matrix Protein, Chanyong Jang Jan 2019

Strain-Specific Protein Interaction And Localization Of Two Strains Of Potato Yellow Dwarf Virus And Functional Domains Of Their Matrix Protein, Chanyong Jang

Theses and Dissertations--Plant Pathology

Potato yellow dwarf virus (PYDV) is the type species of the genus nucleorhabdovirus which is typified by its nucleotropic characters of the members. The virus accomplishes its replication and morphogenesis in the nuclei of infected cells. Two strains, Constricta strain (CYDV) and Sanguinolenta strain (SYDV) have been described at the level of vector-specificity. CYDV is vectored by Agallia constricta and SYDV is transmitted by Aceratagllia sanguinolenta. The full-length genome of CYDV was sequenced. The 12,792 nt antisense genome encodes seven open reading frames in the order of, nucleocapsid protein (N), unknown protein (X), phosphoprotein (P), movement protein (Y), matrix ...


Forage News [2019-01], University Of Kentucky Department Of Plant And Soil Sciences Jan 2019

Planting Date, Hybrid Maturity, And Weather Effects On Maize Yield And Crop Stage, M. E. Baum, S. V. Archontoulis, M. A. Licht Jan 2019

Planting Date, Hybrid Maturity, And Weather Effects On Maize Yield And Crop Stage, M. E. Baum, S. V. Archontoulis, M. A. Licht

Agronomy Publications

Unfavorable weather conditions frequently cause farmers to plant maize (Zea mays L.) outside the optimum planting timeframe. We analyzed maize yield and phenology from a multilocation, year, hybrid relative maturity, and planting date experiment performed in Iowa, USA. Our objectives were to determine the optimum combination of planting date and relative maturity to maximize maize grain yield per environment and to elucidate the risk associated with the use of “full-season hybrids” when planting occurs beyond the optimum planting date. Analysis of variance (ANOVA) attributed 70% of the variability in grain yield to planting date and only 10% to relative maturity ...


Leaf Nutrients, Not Specific Leaf Area, Are Consistent Indicators Of Elevated Nutrient Inputs, Jennifer Firn, Kimberly J. La Pierre, Lori Biederman, Et Al. Jan 2019

Leaf Nutrients, Not Specific Leaf Area, Are Consistent Indicators Of Elevated Nutrient Inputs, Jennifer Firn, Kimberly J. La Pierre, Lori Biederman, Et Al.

Ecology, Evolution and Organismal Biology Publications

Leaf traits are frequently measured in ecology to provide a ‘common currency’ for predicting how anthropogenic pressures impact ecosystem function. Here, we test whether leaf traits consistently respond to experimental treatments across 27 globally distributed grassland sites across 4 continents. We find that specific leaf area (leaf area per unit mass)—a commonly measured morphological trait inferring shifts between plant growth strategies—did not respond to up to four years of soil nutrient additions. Leaf nitrogen, phosphorus and potassium concentrations increased in response to the addition of each respective soil nutrient. We found few significant changes in leaf traits when ...


Three Sister Crops: Understanding American Indian Agricultural Practices Of Corn, Beans And Squash, Sara Colombe, Madhav P. Nepal, Larry B. Browning, Matthew L. Miller, P. Troy White Dec 2018

Three Sister Crops: Understanding American Indian Agricultural Practices Of Corn, Beans And Squash, Sara Colombe, Madhav P. Nepal, Larry B. Browning, Matthew L. Miller, P. Troy White

iLEARN Curriculum Resources

American Indians have practiced an inter-planting system to produce corn, beans, and squash, for generations. These crops are known as the “Three Sisters”. In this lesson developed for secondary agriscience curriculum, students will understand the past, current and future production practices of the three important crops. Students will also apply their knowledge to understand the crop selection process and relate to the changing environment.


Multi-Tactic Ecological Weed Management In A Changing Climate, Sonja K. Birthisel Dec 2018

Multi-Tactic Ecological Weed Management In A Changing Climate, Sonja K. Birthisel

Electronic Theses and Dissertations

Climate change is expected to impact weed communities in Maine, and the efficacy of tools and tactics farmers use to manage them. Through seedbank sampling and surveys of Maine organic farms, we identified currently rare weeds that are known to be especially abundant or problematic in warmer areas of the USA and might therefore represent an emerging agronomic risk. Many ecological weed management strategies that focus on depleting the weed seedbank are expected to remain effective in a changing climate, and become increasingly important as efficacy of cultivation and some herbicide applications diminish or become more variable. Through field experiments ...


Living Shoreline Stabilization To Increase Resiliency At De Soto National Memorial, Linda Walters, Melinda Donnelly, Christian Pilato, Suzanne Connor Dec 2018

Living Shoreline Stabilization To Increase Resiliency At De Soto National Memorial, Linda Walters, Melinda Donnelly, Christian Pilato, Suzanne Connor

CEELAB Research Data

De Soto National Memorial (Bradenton, FL) is an important location where both Native Americans and De Soto's army of soldiers once lived. We have been working closely with the National Park Service and the University of Central Florida to understand how to best stabilize this shoreline using living shoreline techniques combined with long-term natural recruitment. Stabilizing De Soto's shoreline is essential to decrease erosion and shoreline loss to protect the Park's cultural and natural resources, especially the numerous historically significant shell middens. Through pre-restoration monitoring, we determined that high-energy boat wakes were limiting natural mangrove recruitment. Lack ...


Establishment Of Turfgrass From Seed And Sod In Sand Based Systems Combining Sprinkler And Subsurface Drip Irrigation Systems, Roshaan Ahmed Khan Niazi Dec 2018

Establishment Of Turfgrass From Seed And Sod In Sand Based Systems Combining Sprinkler And Subsurface Drip Irrigation Systems, Roshaan Ahmed Khan Niazi

Electronic Theses and Dissertations

The establishment of turfgrass using subsurface drip irrigation (SDI) in sand based media has limited success. However, it is unknown if starting with overhead (sprinkler) irrigation and transitioning to SDI can enhance turfgrass establishment. Two experiments were conducted at Stephen F. Austin State University (SFASU) to investigate the establishment of “L-93” creeping bentgrass (Agrostis stolonifera) from seed and “TifEagle” ultra dwarf hybrid bermudagrass (Cynodon dactylon x Cynodon transvaalensis) from sod using sand based media. Each experiment consisted of two trials conducted in summer 2017 and spring 2018. Both creeping bentgrass trials and the spring ultra dwarf hybrid bermudagrass trial were ...