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2002

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Full-Text Articles in Life Sciences

The Novel Presenilin-1-Associated Protein Is A Proapoptotic Mitochondrial Protein, Xuemin Xu, Yc Shi, W Gao, G Mao, G Zhao, S Agrawal, Gm Chisolm, D Sui, Mei-Zhen Cui Jun 2013

The Novel Presenilin-1-Associated Protein Is A Proapoptotic Mitochondrial Protein, Xuemin Xu, Yc Shi, W Gao, G Mao, G Zhao, S Agrawal, Gm Chisolm, D Sui, Mei-Zhen Cui

Mei-Zhen Cui

Recent studies have suggested a possible role for presenilin proteins in apoptotic cell death observed in Alzheimer's disease. The mechanism by which presenilin proteins regulate apoptotic cell death is not well understood. Using the yeast two-hybrid system, we previously isolated a novel protein, presenilin-associated protein (PSAP) that specifically interacts with the C terminus of presenilin 1 (PS1), but not presenilin 2 (PS2). Here we report that PSAP is a mitochondrial resident protein sharing homology with mitochondrial carrier protein. PSAP was detected in a mitochondria-enriched fraction, and PSAP immunofluorescence was present in a punctate pattern that colocalized with a mitochondrial ...


Detection Of Feline Coronavirus In Captive Felidae In The Usa, Melissa Kennedy, S Citino, A Mcnabb, A Moffatt, K Gertz, Stephen Kania Mar 2012

Detection Of Feline Coronavirus In Captive Felidae In The Usa, Melissa Kennedy, S Citino, A Mcnabb, A Moffatt, K Gertz, Stephen Kania

Stephen A Kania

Feline coronavirus (FCoV) is an important pathogen of domestic and nondomestic Felidae. Investigation into the prevalence of FCoV in exotic Felidae has relied primarily on serology. The usefulness of genetic detection of FCoV using reverse transcription and nested polymerase chain reaction (RT/nPCR) for viral screening was investigated. Seventy-five biologic samples, primarily feces, from captive felids from 11 institutions were tested using PCR. Serum samples collected from all but 12 of these animals were tested for antibodies to type I and type II FCoV by indirect immunofluorescence. Twenty-four animals were positive using RT/nPCR for virus. Twenty-nine animals were seropositive ...


The Role Of The Lissencephaly Protein Pac1 During Nuclear Migration In Budding Yeast, Wei-Lih Lee, J. R. Oberle, J. A. Cooper Dec 2002

The Role Of The Lissencephaly Protein Pac1 During Nuclear Migration In Budding Yeast, Wei-Lih Lee, J. R. Oberle, J. A. Cooper

Wei-Lih Lee

During mitosis in Saccharomyces cerevisiae, the mitotic spindle moves into the mother–bud neck via dynein-dependent sliding of cytoplasmic microtubules along the cortex of the bud. Here we show that Pac1, the yeast homologue of the human lissencephaly protein LIS1, plays a key role in this process. First, genetic interactions placed Pac1 in the dynein/dynactin pathway. Second, cells lacking Pac1 failed to display microtubule sliding in the bud, resulting in defective mitotic spindle movement and nuclear segregation. Third, Pac1 localized to the plus ends (distal tips) of cytoplasmic microtubules in the bud. This localization did not depend on the ...


Community Conservation Assistance, Frank Mitchell Dec 2002

Community Conservation Assistance, Frank Mitchell

PREP Reports & Publications

The Community Conservation Assistance Project had the following goal and objectives: Goal: Sustain and protect open space lands identified by communities and land trusts as critical to water quality and biodiversity through voluntary land conservation. Objectives: 1. With the SPNHF and other partners, establish a seacoast office for the Center for Land Conservation Assistance. 2. Provide education to various audiences in the seacoast region. 3. Create a library of relevant materials 4. Develop and compile publications 5. Establish with the SPNHF and other partners and participate in the first annual NH Land Conservation Conference 6. Evaluate program effectiveness 7. Coordinate ...


Shellfish Population And Bed Dimension Assessment In The Great Bay Estuary, Brian Smith Dec 2002

Shellfish Population And Bed Dimension Assessment In The Great Bay Estuary, Brian Smith

PREP Reports & Publications

This final report details a study funded by the NHEP over the course of 2001-02. The NHF&G Department was funded to delineate oyster bed size and density, monitor oyster disease, and examine the density of clam concentrations in the Great Bay Estuary.


Viability Of A Drug-Resistant Human Immunodeficiency Virus Type 1 Protease Variant: Structural Insights For Better Antiviral Therapy, Moses Prabu-Jeyabalan, Ellen A. Nalivaika, Nancy M. King, Celia A. Schiffer Dec 2002

Viability Of A Drug-Resistant Human Immunodeficiency Virus Type 1 Protease Variant: Structural Insights For Better Antiviral Therapy, Moses Prabu-Jeyabalan, Ellen A. Nalivaika, Nancy M. King, Celia A. Schiffer

Open Access Articles

Under the selective pressure of protease inhibitor therapy, patients infected with human immunodeficiency virus (HIV) often develop drug-resistant HIV strains. One of the first drug-resistant mutations to arise in the protease, particularly in patients receiving indinavir or ritonavir treatment, is V82A, which compromises the binding of these and other inhibitors but allows the virus to remain viable. To probe this drug resistance, we solved the crystal structures of three natural substrates and two commercial drugs in complex with an inactive drug-resistant mutant (D25N/V82A) HIV-1 protease. Through structural analysis and comparison of the protein-ligand interactions, we found that Val82 interacts ...


Environmental Indicator Report: Water Quality, Phil Trowbridge Dec 2002

Environmental Indicator Report: Water Quality, Phil Trowbridge

PREP Reports & Publications

During the fall and winter of 2001-2002, the New Hampshire Estuaries Project’s Technical Advisory Committee (TAC) developed a suite of environmental indicators to track progress toward the NHEP’s management goals and objectives. These indicators were fully described in terms of their performance criteria, statistical methods, and measurable goals in the NHEP’s Monitoring Plan published in May 2002. The next step is to use these indicators to produce an updated “State of the Estuaries” report by the spring of 2003. The TAC decided to break this task into three sections: shellfish indicators in the fall of 2002; water ...


Juvenile Clam Mortality Study At Three Intertidal Flats In Hampton Harbor, New Hampshire, Brian F. Beal Dec 2002

Juvenile Clam Mortality Study At Three Intertidal Flats In Hampton Harbor, New Hampshire, Brian F. Beal

PREP Reports & Publications

Soft-shell clams, Mya arenaria L., represent an important recreational fishery along the New Hampshire coast. Intertidal flats in the Hampton-Seabrook Estuary are among the most heavily harvested. As recently as the fall of 1998, the sandy flats in this area supported more than 900 clammers, who, on weekends, easily harvested their 9.5-liter limit (Varney, 1999). Recently, however, quantitative benthic surveys have suggested that the abundance of adult clams (> 50 mm shell length, SL) has dwindled on the three estuarine flats (Brown’s Flat, Common Island, and Middle Ground). Clammers support this contention and many have been disappointed at the ...


Differential Lipid Biosynthesis Underlies A Tradeoff Between Reproduction And Fl Ight Capability In A Wing-Polymorphic Cricket, Zhangwu Zhao, Anthony J. Zera Dec 2002

Differential Lipid Biosynthesis Underlies A Tradeoff Between Reproduction And Fl Ight Capability In A Wing-Polymorphic Cricket, Zhangwu Zhao, Anthony J. Zera

Anthony Zera Publications

The biochemical basis of life-history tradeoffs is a poorly studied aspect of life-history evolution. We used radiotracer and endocrine approaches to investigate the extent to which morphs of a wing-polymorphic insect differ in the biosynthesis of lipid classes important for dispersal capability vs. reproduction (ovarian growth). The flightcapable genotype of Gryllus firmus biosynthesized a greater amount of total lipid and triglyceride (main flight fuel), which was preferentially allocated to somatic tissue during early adulthood. By contrast, the fl ightless genotype biosynthesized a significantly greater amount of phospholipid (important in egg development), which was preferentially allocated to ovaries. Topical application of ...


Certified Crop Adviser Short Courses In 2003, Richard Larson Dec 2002

Certified Crop Adviser Short Courses In 2003, Richard Larson

Integrated Crop Management News

Several certified crop adviser (CCA) short courses will be offered starting in February. All participants must be preregistered before the day of the school. Courses will be taught at various locations in Ames. Please call (515) 294-6429 for registration information.


Rootworm Insecticides Evaluated, Marlin E. Rice, James Oleson Dec 2002

Rootworm Insecticides Evaluated, Marlin E. Rice, James Oleson

Integrated Crop Management News

Two integrated pest management strategies are commonly used to protect corn roots from corn rootworm injury: crop rotation and insecticides. If corn is not rotated, or if extended diapause northern corn rootworms occur in a field, a soil insecticide might be necessary to protect the roots in 2003. The reason we say it might be necessary is because many fields do not have a rootworm population of a sufficient size to cause economic damage. There are thousands of continuous cornfields across Iowa in which a rootworm insecticide is not used and is not necessary.


Crop Adviser Institute Is Online, Brent Brueland Dec 2002

Crop Adviser Institute Is Online, Brent Brueland

Integrated Crop Management News

The Crop Adviser Institute (CAI), a new distance education institute at Iowa State University, is now online and delivering continuing education courses to ag professionals in Iowa and throughout North America. Ag professionals are interested in advanced and continuing education, whether it is to fulfill a requirement for certification or solely as a supplemental educational opportunity. Agricultural continuing education has traditionally been delivered on-site, but temporal, geographical, and other constraints often prevent individuals from attending these courses.


Volunteer For Iowater In 2003, Mahdi Al-Kaisi, H. Mark Hanna, Lynette Seigley, Michael J. Tidman Dec 2002

Volunteer For Iowater In 2003, Mahdi Al-Kaisi, H. Mark Hanna, Lynette Seigley, Michael J. Tidman

Integrated Crop Management News

Water quality is an important issue for agriculture. The protection of Iowa's rivers, streams, and lakes from nonpoint source pollution is one of the greatest environmental challenges facing our state. Despite the accomplishments of Iowa producers over the past few years (buffer strips, grassed waterways, no-till, and similar efforts), some water bodies have fallen short of water quality standards.


Extra 2002 Icm Conference Proceedings, Richard Larson Dec 2002

Extra 2002 Icm Conference Proceedings, Richard Larson

Integrated Crop Management News

The Agribusiness Education Program office has extra copies of the 2002 Integrated Crop Management Conference proceedings available for purchase. If you pick up copies at the office, the cost is $15 each. If copies are shipped to you, they are $17.50 each and this price includes the shipping cost. To place an order, make your check payable to Iowa State University, or provide your credit card number, expiration date, and cardholder name if paying by credit card (Visa/MasterCard/Discover) and mail it to Agribusiness Education Program, 2104 Agronomy Hall, Iowa State University, Ames, Iowa, 50011.


Autoantibodies To Centrosomes Are Diagnostic For Human Scleroderma And Can Be Induced By Experimental Mycoplasma Infection In Mice: A Dissertation, Irina Catrinel Gavanescu Dec 2002

Autoantibodies To Centrosomes Are Diagnostic For Human Scleroderma And Can Be Induced By Experimental Mycoplasma Infection In Mice: A Dissertation, Irina Catrinel Gavanescu

GSBS Dissertations and Theses

The overall objective of this thesis work was to develop new insights into the etiology of scleroderma, a human systemic autoimmune disease, by analyzing the autoantibodies to centrosome antigens that develop during the disease. Centrosomes are perinuclear organelles that form microtubule arrays, including mitotic spindles that ensure the faithful segregation of chromosomes during mitosis.

These studies used a novel methodology to determine the prevalence of anti-centrosome autoantibodies in patients with scleroderma. Recombinant centrosome antigens were used to determine the antigenic specificity of anti-centrosome antibody subsets by immunoblotting. Centrosome marker antibodies were used in indirect immunofluorescence assays to distinguish centrosomes within ...


Estimation Of Relative Abundance Of Recreationally Important Juvenile Finfish In The Virginia Portion Of Chesapeake Bay Project Rfol-3 July 2001 - June 2002, Marcel M. Montaine, Herbert M. Austin, Patrick J. Geer, Wendy A. Lowery Dec 2002

Estimation Of Relative Abundance Of Recreationally Important Juvenile Finfish In The Virginia Portion Of Chesapeake Bay Project Rfol-3 July 2001 - June 2002, Marcel M. Montaine, Herbert M. Austin, Patrick J. Geer, Wendy A. Lowery

Reports

No abstract provided.


Estimating The Latitudinal Origins Of Migratory Birds Using Hydrogen And Sulfur Stable Isotopes In Feathers: Influence Of Marine Prey Base, Casey A. Lott, Timothy D. Meehan, Julie A. Heath Dec 2002

Estimating The Latitudinal Origins Of Migratory Birds Using Hydrogen And Sulfur Stable Isotopes In Feathers: Influence Of Marine Prey Base, Casey A. Lott, Timothy D. Meehan, Julie A. Heath

Julie Heath

Hydrogen stable isotope analysis of feathers is an important tool for estimating the natal or breeding latitudes of nearctic-neotropical migratory birds. This method is based on the latitudinal variation of hydrogen stable isotope ratios in precipitation in North America (iDp) and the inheritance of this variation in newly formed feathers (iDf). We hypothesized that the typically strong relationship between iDp and iDf would be decoupled in birds that forage in marine food webs because marine waters have relatively high iD values compared to iD values for local precipitation. Birds that forage on marine prey bases should also have feathers with ...


Surface And Deep Ocean Circulation In The Subpolar North Atlantic During The Mid-Pleistocene Revolution, Amy K. Wright, Benjamin P. Flower Dec 2002

Surface And Deep Ocean Circulation In The Subpolar North Atlantic During The Mid-Pleistocene Revolution, Amy K. Wright, Benjamin P. Flower

Marine Science Faculty Publications

We investigated surface and deep ocean variability in the subpolar North Atlantic from 1000 to 500 thousand years ago (ka) based on two Ocean Drilling Program (ODP) sites, Feni drift site 980 (55°29′N, 14°42′W) and Bjorn drift site 984 (61°25′N, 24°04′W). Benthic foraminiferal stable isotope data, planktic foraminiferal faunas, ice‐rafted debris data, and faunally based sea‐surface temperature estimates help test the hypothesis that oceanographic changes in the North Atlantic region were associated with the onset of the 100‐kyr world during the mid‐Pleistocene revolution. Based on percentage of Neogloboquadrina ...


Sea Surface Salinity Measurements In The Historical Database, Frederick M. Bingham, Stephan D. Howden, Chester J. Koblinsky Dec 2002

Sea Surface Salinity Measurements In The Historical Database, Frederick M. Bingham, Stephan D. Howden, Chester J. Koblinsky

Faculty Publications

We have examined historical distributions of sea surface salinity (SSS) observations in a data set consisting of a combination of the World Ocean Database 1998 (WOD98) and a thermosalinograph and bucket salinity database collected from volunteer observing ships. It is well known that SSS in much of the world's ocean is measured infrequently or not at all. We find that 27% of one-degree squares in the world ocean (open and coastal, excluding the Arctic Ocean) had no observations of SSS in the historical database, and 70% had 10 or fewer. Systematic sampling of SSS (more than 10,000 observations ...


Northeast Research Station Watertown, South Dakota Annual Progress Report, 2002, Agricultural Experiment Station, Plant Science Department Dec 2002

Northeast Research Station Watertown, South Dakota Annual Progress Report, 2002, Agricultural Experiment Station, Plant Science Department

Agricultural Experiment Station and Research Farm Annual Reports

This is the 2002 annual progress report for the Northeast Research Station in Watertown, South Dakota. This report is issued by the South Dakota Agricultural Experiment Station and the South Dakota State University Plant Science Department. This report includes weather data, yield comparisons, crop performance trials, corn trials, soybean trials, winter wheat performance testing, Barley Foliar information, weed and pest control, herbicide demonstrations, canola and flax variety trials and more.


Evidence For An Epigenetic Mechanism By Which Hsp90 Acts As A Capacitor For Morphological Evolution, Vincent E. Sollars, Xiangyi Lu, Li Xiao, Xiaoyan Wang, Mark D. Garfinkel, Douglas M. Ruden Dec 2002

Evidence For An Epigenetic Mechanism By Which Hsp90 Acts As A Capacitor For Morphological Evolution, Vincent E. Sollars, Xiangyi Lu, Li Xiao, Xiaoyan Wang, Mark D. Garfinkel, Douglas M. Ruden

Biochemistry and Microbiology

Morphological alterations have been shown to occur in Drosophila melanogaster when function of Hsp90 (heat shock 0-kDa protein 1α, encoded by Hsp83) is compromised during development1. Genetic selection maintains the altered phenotypes in subsequent generations1. Recent experiments have shown, however, that phenotypic variation still occurs in nearly isogenic recombinant inbred strains of Arabidopsis thaliana2. Using a sensitized isogenic D. melanogaster strain, iso-KrIf-1, we confirm this finding and present evidence supporting an epigenetic mechanism for Hsp90’s capacitor function, whereby reduced activity of Hsp90 induces a heritably altered chromatin state. The altered chromatin state is evidenced by ectopic expression ...


Evaluation Of Striped Bass Stocks In Virginia, Monitoring And Tagging Studies, 1999-2003 Annual Report, 1 September 2001 - 31 October 2002, Philip W. Sadler, Robert J. Latour, Robert E. Harris, Kristin L. Maki, John E. Olney Dec 2002

Evaluation Of Striped Bass Stocks In Virginia, Monitoring And Tagging Studies, 1999-2003 Annual Report, 1 September 2001 - 31 October 2002, Philip W. Sadler, Robert J. Latour, Robert E. Harris, Kristin L. Maki, John E. Olney

Reports

This report presents the results of striped bass (Marone saxatilis) tagging and monitoring activities in Virginia during the penod 1 September 2001 through 31 October 2002. It includes an assessment of the biological characteristics of striped bass taken from the 2002 spring spawning run, estimates of annual survtval based on annual spring tagging, and the results of the fall 2001 directed mortality study that is cooperative with the Maryland Department of Natural Resources. The information contained in this report is required by the Atlantic States Marine Fisheries Commission and is used to implement a coordinated management plan for striped bass ...


West River Ag Center Crops And Soils Research Annual Progress Report, 2002, Agricultural Experiment Station Dec 2002

West River Ag Center Crops And Soils Research Annual Progress Report, 2002, Agricultural Experiment Station

Agricultural Experiment Station and Research Farm Annual Reports

This is the 2002 progress report of the West River Crops and Soils Research Projects, South Dakota Agricultural Experiment Station. This document includes reports on: weather and climate, wheat and grain variety trials, management and tillage, and weed and pest control.


The Effects Of Prescribed Fire On Millipede And Salamander Populations In A Southern Appalachian Deciduous Forest., Alison Baird Gagan Dec 2002

The Effects Of Prescribed Fire On Millipede And Salamander Populations In A Southern Appalachian Deciduous Forest., Alison Baird Gagan

Electronic Theses and Dissertations

Prescribed fire has increased as a forest management practice in southern Appalachia, but investigations into the effects of this silvicultural treatment on non-game wildlife inhabiting the region is limited. This study investigated the effects of prescribed fire on millipede and salamander populations. Seventeen sites within the Cherokee National Forest in east Tennessee that were treated once with prescribed fire between 1998 and 2002 were examined in the spring of 2002. Each burned plot was paired with an adjacent unburned plot.

The number of individual millipedes and salamanders collected from burned plots was compared to the corresponding control plot. Millipedes declined ...


Cases, Regulations, And Statutes, Robert P. Achenbach Jr Dec 2002

Cases, Regulations, And Statutes, Robert P. Achenbach Jr

Agricultural Law Digest

No abstract provided.


When Are Livestock Exchanges "Like-Kind"?, Neil E. Harl Dec 2002

When Are Livestock Exchanges "Like-Kind"?, Neil E. Harl

Agricultural Law Digest

Although less common than like-kind exchanges of real estate or machinery, exchanges of livestock appear to be occurring more frequently in recent years. Except for the statutory bar for exchanges of livestock of different sexes, the rules governing livestock exchanges are less well known. The regulations adopted in 1991 have provided more definitive guidelines for like-kind exchanges of livestock (and other assets) than were available previously.


Index To Volume 13, Nos. 1-24, Agricultural Law Digest Dec 2002

Index To Volume 13, Nos. 1-24, Agricultural Law Digest

Agricultural Law Digest

No abstract provided.


The Novel Presenilin-1-Associated Protein Is A Proapoptotic Mitochondrial Protein, Xuemin Xu, Yc Shi, W Gao, G Mao, G Zhao, S Agrawal, Gm Chisolm, D Sui, Mei-Zhen Cui Dec 2002

The Novel Presenilin-1-Associated Protein Is A Proapoptotic Mitochondrial Protein, Xuemin Xu, Yc Shi, W Gao, G Mao, G Zhao, S Agrawal, Gm Chisolm, D Sui, Mei-Zhen Cui

Xuemin Xu

Recent studies have suggested a possible role for presenilin proteins in apoptotic cell death observed in Alzheimer's disease. The mechanism by which presenilin proteins regulate apoptotic cell death is not well understood. Using the yeast two-hybrid system, we previously isolated a novel protein, presenilin-associated protein (PSAP) that specifically interacts with the C terminus of presenilin 1 (PS1), but not presenilin 2 (PS2). Here we report that PSAP is a mitochondrial resident protein sharing homology with mitochondrial carrier protein. PSAP was detected in a mitochondria-enriched fraction, and PSAP immunofluorescence was present in a punctate pattern that colocalized with a mitochondrial ...


Epigenetic History Of An Arabidopsis Trans-Silencer Locus And A Test For Relay Of Trans-Silencing Activity, Huaxia Qin, Albrecht G. Von Arnim Dec 2002

Epigenetic History Of An Arabidopsis Trans-Silencer Locus And A Test For Relay Of Trans-Silencing Activity, Huaxia Qin, Albrecht G. Von Arnim

Plant Sciences Publications and Other Works

Background

Meiotically heritable epimutations affecting transgene expression are not well understood, even and in particular in the plant model species, Arabidopsis thaliana. The Arabidopsis trans-silencer locus, C73, which encodes a fusion protein between the repressor of photomorphogenesis, COP1, and green fluorescent protein (GFP-COP1), heritably modifies the expression pattern and cop1-like cosuppression phenotypes of multiple GFP-COP1 target loci by transcriptional gene silencing.

Results

Here we describe three additional features of trans-silencing by the C73 locus. First, the silencing phenotype of C73 and of similar complex loci was acquired epigenetically over the course of no more than two plant generations via ...


What Makes An Animal Choose A Forage, Elizabeth A. Burritt, Frederick D. Provenza Dec 2002

What Makes An Animal Choose A Forage, Elizabeth A. Burritt, Frederick D. Provenza

Wildland Resources Faculty Publications

Consider the following scenarios: To cut the costs of ranching a researcher explores feeding cattle ammoniated straw in winter. Some of the cows maintain themselves on the diet while others lose weight, produce less milk and fail to conceive. A producer in Missouri plants a pasture rich in legumes and high in crude protein, yet his cattle prefer moldy hay and endophyte infected tall fescue to the legumes. Why do animals behave this way? Animals are thought to prefer foods that are palatable but what is palatability? We define palatability as the interrelationship between a food's flavor and postingestive ...