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2008

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Full-Text Articles in Life Sciences

Gene Signature Of Stromal Cells Which Support Dendritic Cell Development., Geneviève Despars, Pravin Periasamy, Jonathan Tan, Janice Abbey, Terence O'Neill, Helen O'Neill Sep 2015

Gene Signature Of Stromal Cells Which Support Dendritic Cell Development., Geneviève Despars, Pravin Periasamy, Jonathan Tan, Janice Abbey, Terence O'Neill, Helen O'Neill

Jonathan Tan

Spleen stromal cells are critical determinants of dendritic cell (DC) development in spleen. The spleen stromal line, namely STX3, supports DC differentiation in vitro from overlaid bone marrow cells while the lymph node stromal line, namely 2RL22, does not. Here we have characterised the hematopoietic support capacity of each stroma, and analyzed lineage origin of the stromal cell lines by gene profiling using microarrays. Stromal co-culture experiments were performed using bone marrow cells as a source of hematopoietic progenitors. A characteristic immature myeloid-like CD11c+CD11b+CD86+MHC-II−/loB220−CD8α− DC is produced after 14 days in STX3 cocultures, while 2RL22 ...


North Atlantic Vibrio Vulnificus Surveillance From Postharvest Oysters At A Us Shellfish Processing Facility, Kenneth Lavalley, Joseph Dealteris, Michael Rice, Marta Gomez-Chiarri Dec 2013

North Atlantic Vibrio Vulnificus Surveillance From Postharvest Oysters At A Us Shellfish Processing Facility, Kenneth Lavalley, Joseph Dealteris, Michael Rice, Marta Gomez-Chiarri

Marta Gomez-Chiarri

Postharvest surveillance for Vibrio vulnificus by a commercial processing facility was conducted from May 2001 to September 2003. Harvest areas included the Delaware Bay, Long Island Sound and Prince Edward Island. Occurrence followed a seasonal distribution. Low densities were observed in June, increased through August, and became rare by September. Given the ubiquitous nature of marine Vibrios, it was not surprising to find V. vulnificus everywhere, which was investigated. Observations confirmed the importance of strict time/temperature and product handling practices by shellfish harvesters, dealers and processors. Mishandling led to a potentially dangerous and uncharacteristically high V. vulnificus observation.


Histamine Induces Egr-1 Expression In Human Aortic Endothelial Cells Via The H1 Receptor-Mediated Protein Kinase Cdelta-Dependent Erk Activation Pathway, F Hao, M Tan, Xuemin Xu, Mei-Zhen Cui Jun 2013

Histamine Induces Egr-1 Expression In Human Aortic Endothelial Cells Via The H1 Receptor-Mediated Protein Kinase Cdelta-Dependent Erk Activation Pathway, F Hao, M Tan, Xuemin Xu, Mei-Zhen Cui

Mei-Zhen Cui

Histamine, a potent inflammatory mediator, has multiple effects on the pathogenesis of atherosclerosis. This study investigates the effect of histamine on the expression of early growth response factor 1 (Egr-1), a master transcription factor that regulates the expression of an array of atherogenic genes in atherosclerotic lesions. Histamine markedly and rapidly induces Egr-1 mRNA and protein expression in primary human aortic endothelial cells (HAECs). Histamine-induced Egr-1 expression is dependent on the activation of the H1 receptor. Histamine also rapidly and transiently activates protein kinase C-delta (PKCdelta), extracellular signal-regulated kinase (ERK)1/2, p38 kinase, and c-Jun N-terminal kinase (JNK) prior ...


Effects Of Gamma-Secretase Cleavage-Region Mutations On App Processing And Abeta Formation: Interpretation With Sequential Cleavage And Alpha-Helical Model, Jianxin Tan, Guozhang Mao, Mei-Zhen Cui, Shin-Chung Kang, Bruce Lamb, Boon-Seng Wong, Man-Sun Sy, Xuemin Xu Jun 2013

Effects Of Gamma-Secretase Cleavage-Region Mutations On App Processing And Abeta Formation: Interpretation With Sequential Cleavage And Alpha-Helical Model, Jianxin Tan, Guozhang Mao, Mei-Zhen Cui, Shin-Chung Kang, Bruce Lamb, Boon-Seng Wong, Man-Sun Sy, Xuemin Xu

Mei-Zhen Cui

Overwhelming evidence supports the amyloid hypothesis of Alzheimer's disease that stipulates that the relative level of the 42 amino acid beta-amyloid peptide (Abeta(42)) in relationship to Abeta(40) is critical to the pathogenesis of the disease. While it is clear that the multi-subunit gamma secretase is responsible for cleavage of the amyloid precursor protein (APP) into Abeta(42) and Abeta(40), the exact molecular mechanisms regulating the production of the various Abeta species remain elusive. To elucidate the underlying mechanisms, we replaced individual amino acid residues from positions 43 to 52 of Abeta with phenylalanine to examine the ...


Both The N-Terminal Fragment And The Protein-Protein Interaction Domain (Pdz Domain) Are Required For The Pro-Apoptotic Activity Of Presenilin-Associated Protein Psap, Guozhang Mao, Jianxin Tan, W Gao, Y Shi, Mei-Zhen Cui, Xuemin Xu Jun 2013

Both The N-Terminal Fragment And The Protein-Protein Interaction Domain (Pdz Domain) Are Required For The Pro-Apoptotic Activity Of Presenilin-Associated Protein Psap, Guozhang Mao, Jianxin Tan, W Gao, Y Shi, Mei-Zhen Cui, Xuemin Xu

Mei-Zhen Cui

Presenilin-associated protein (PSAP) was originally identified as a PS1-associated, PDZ domain protein. In a subsequent study, PSAP was found to be a mitochondrial apoptotic molecule. In this study, we cloned the PSAP gene and found that it is composed of 12 exons and localizes on chromosome 6. To better understand the structure and function of PSAP, we have generated a series of antibodies that recognize different regions of PSAP. Using these antibodies, we found that PSAP is expressed in four isoforms as a result of differential splicing of exon 8 in addition to the use of either the first or ...


A Simple Spectrophotometric Streptavidin-Biotin Binding Assay Utilizing Biotin-4-Fluorescein., Mark Waner, David Mascotti May 2013

A Simple Spectrophotometric Streptavidin-Biotin Binding Assay Utilizing Biotin-4-Fluorescein., Mark Waner, David Mascotti

Mark J. Waner

A new assay for biotin binding capacity of Streptavidin (SA) is presented in this work. The assay is based on the large decrease in the extinction coefficient at 493 nm that accompanies binding of biotin-4-fluorescein (B4F) to SA. This decrease is attributed to formation of a charge transfer complex between the B4F-donor and one or more SA residues. We show that one may observe the stoichiometric binding via monitoring the absorbance at 493 nm using either SA or B4F as the titrant. The sensitivity of the assay is at the lower end of similar fluorimetric and photometric assays. Though the ...


Proteome Of Geobacter Sulfurreducens Through Genome-Wide Expression Data And Sequence Analysis, Derek Lovley, Yan-Huai Ding, Kim Hixson, Muktak Aklujkar, Mary Lipton, Richard Smith, Tϋnde Mester Mar 2012

Proteome Of Geobacter Sulfurreducens Through Genome-Wide Expression Data And Sequence Analysis, Derek Lovley, Yan-Huai Ding, Kim Hixson, Muktak Aklujkar, Mary Lipton, Richard Smith, Tϋnde Mester

Muktak Aklujkar

No abstract provided.


Evaluation Of Antibodies Against Feline Coronavirus 7b Protein For Diagnosis Of Feline Infectious Peritonitis In Cats, Melissa Kennedy, M Abd-Eldaim, S Zika, Joseph Mankin, Stephen Kania Jan 2012

Evaluation Of Antibodies Against Feline Coronavirus 7b Protein For Diagnosis Of Feline Infectious Peritonitis In Cats, Melissa Kennedy, M Abd-Eldaim, S Zika, Joseph Mankin, Stephen Kania

Melissa A. Kennedy

OBJECTIVE: To determine whether expression of feline coronavirus (FCoV) 7b protein, as indicated by the presence of specific serum antibodies, consistently correlated with occurrence of feline infectious peritonitis (FIP) in cats. SAMPLE POPULATION: 95 serum samples submitted for various diagnostic assays and 20 samples from specific-pathogen-free cats tested as negative control samples. PROCEDURES: The 7b gene from a virulent strain of FCoV was cloned into a protein expression vector. The resultant recombinant protein was produced and used in antibody detection assays via western blot analysis of serum samples. Results were compared with those of an immunofluorescence assay (IFA) for FCoV-specific ...


Molecular Characterisation Of Canine Nonsteroidal Anti-Inflammatory Drug-Activated Gene (Nag-1), K Yamaguchi, Nichelle Whitlock, Jason Liggett, Alfred Legendre, Michael Fry, Seung Baek Dec 2010

Molecular Characterisation Of Canine Nonsteroidal Anti-Inflammatory Drug-Activated Gene (Nag-1), K Yamaguchi, Nichelle Whitlock, Jason Liggett, Alfred Legendre, Michael Fry, Seung Baek

Alfred M Legendre DVM, MS, DACVIM

Nonsteroidal anti-inflammatory drug (NSAID)-activated gene (NAG-1), a divergent member of the transforming growth factor beta superfamily, was previously identified as a gene induced by several anti-tumorigenic compounds, including NSAIDs and peroxisome proliferator-activated receptor gamma (PPARgamma) ligands in humans. In this study, canine NAG-1 was characterised from a canine genomic database. Gene induction by some NSAIDs and PPARgamma ligands was demonstrated in canine osteosarcoma cell lines. Phylogenetic analysis indicates that canine NAG-1 is more homologous with the corresponding mouse and rat genes than with human NAG-1. Expression of canine NAG-1 was increased by treatment with piroxicam and SC-560 (NSAIDs) and ...


Multiple Mechanisms Are Involved In 6-Gingerol-Induced Cell Growth Arrest And Apoptosis In Human Colorectal Cancer Cells, Seong Lee, Maria Cekanova, Seung Baek Oct 2010

Multiple Mechanisms Are Involved In 6-Gingerol-Induced Cell Growth Arrest And Apoptosis In Human Colorectal Cancer Cells, Seong Lee, Maria Cekanova, Seung Baek

Maria Cekanova MS, RNDr, PhD

6-Gingerol, a natural product of ginger, has been known to possess anti-tumorigenic and pro-apoptotic activities. However, the mechanisms by which it prevents cancer are not well understood in human colorectal cancer. Cyclin D1 is a proto-oncogene that is overexpressed in many cancers and plays a role in cell proliferation through activation by beta-catenin signaling. Nonsteroidal anti-inflammatory drug (NSAID)-activated gene-1 (NAG-1) is a cytokine associated with pro-apoptotic and anti-tumorigenic properties. In the present study, we examined whether 6-gingerol influences cyclin D1 and NAG-1 expression and determined the mechanisms by which 6-gingerol affects the growth of human colorectal cancer cells in ...


Peroxisome Proliferator-Activated Receptor Ligand Mcc-555 Suppresses Intestinal Polyps In Apcmin/+ Mice Via Extracellular Signal-Regulated Kinase And Peroxisome Proliferator-Activated Receptor-Dependent Pathways, K Yamaguchi, Maria Cekanova, Michael Mcentee, J Yoon, S Fischer, I Renes, I Van Seungnigen, Seung Baek Oct 2010

Peroxisome Proliferator-Activated Receptor Ligand Mcc-555 Suppresses Intestinal Polyps In Apcmin/+ Mice Via Extracellular Signal-Regulated Kinase And Peroxisome Proliferator-Activated Receptor-Dependent Pathways, K Yamaguchi, Maria Cekanova, Michael Mcentee, J Yoon, S Fischer, I Renes, I Van Seungnigen, Seung Baek

Maria Cekanova MS, RNDr, PhD

A large body of studies has suggested that peroxisome proliferator-activated receptor gamma (PPARgamma) ligands, such as thiazolidinedione, are potent candidates for chemopreventive agents. MCC-555 is a PPARgamma/alpha dual agonist and has been shown previously to induce apoptosis in vitro; however, the molecular mechanisms by which MCC-555 affects antitumorigenesis in vivo are poorly understood. In this study, we explored the antitumorigenic effects of MCC-555 both in cell culture and in Apc-deficient mice, an animal model for human familial adenomatous polyposis. MCC-555 increased MUC2 expression in colorectal and lung cancer cells, and treatment with the PPARgamma antagonist GW9662 revealed that MUC2 ...


The 4 X 4 Semantic Model: Exploiting Data, Functional, Non-Functional And Execution Semantics Across Business Process, Workflow, Partner Services And Middleware Services Tiers, Amit P. Sheth, Karthik Gomadam Dec 2008

The 4 X 4 Semantic Model: Exploiting Data, Functional, Non-Functional And Execution Semantics Across Business Process, Workflow, Partner Services And Middleware Services Tiers, Amit P. Sheth, Karthik Gomadam

Kno.e.sis Publications

Business processes in the global environment increasingly encompass multiple partners and complex, rapidly changing requirements. In this context it is critical that strategic business objectives align with and map accurately to systems that support flexible and dynamic business processes. To support the demanding requirements of global business processes, we propose a comprehensive, unifying 4 X 4 Semantic Model that uses Semantic Templates to link four tiers of implementation with four types of semantics. The four tiers are the Business Process Tier, the Workflow Enactment Tier, the Partner Services Tier, and the Middleware Services Tier. The four types of semantics are ...


Assessing The State Of Clinically Applicable Research For Evidence Based Practice In Prosthetics And Orthotics, Mark Geil Dec 2008

Assessing The State Of Clinically Applicable Research For Evidence Based Practice In Prosthetics And Orthotics, Mark Geil

Kinesiology Faculty Publications

Evidence-based practice combines a practitioner’s training and experience with evidence established through scientific research. Fundamental to the evidence-based process for prosthetics and orthotics is the ongoing availability of clinically applicable research on relevant conditions, components, and patient populations. In the past, research has been successfully applied to practice, sometimes producing substantial changes. Examples include clinically applicable research that has assessed treatment effectiveness, altered clinical patient interaction, led to the development of new components and technologies, and challenged or changed long-standing clinical opinion. Despite past successes, obstacles remain in the application of research to practice. Practitioners have stated a desire ...


Automatic Reaction To A Chemical Event Detected By A Low-Cost Wireless Chemical Sensing Network, Stephen Beirne, King Tong Lau, Brian Corcoran, Dermot Diamond Dec 2008

Automatic Reaction To A Chemical Event Detected By A Low-Cost Wireless Chemical Sensing Network, Stephen Beirne, King Tong Lau, Brian Corcoran, Dermot Diamond

Faculty of Science - Papers (Archive)

A test-scale wireless chemical sensor network (WCSN) has been deployed within a controlled Environmental Chamber (EC). The combined signals from the WCSN were used to initiate a controllable response to the detected chemical event. When a particular sensor response pattern was obtained, a purging cycle was initiated. Sensor data were continuously checked against user-defined action limits, to determine if a chemical event had occurred. An acidic contaminant was used to demonstrate the response of the sensor network. Once the acid plume was simultaneously detected by a number of wireless chemical sensor nodes, an automatic response action, which was the purging ...


Total Nitrogen Concentrations In Wastewater Treatment Plant Effluent In The Great Bay Estuary Watershed In 2008, Philip Trowbridge Dec 2008

Total Nitrogen Concentrations In Wastewater Treatment Plant Effluent In The Great Bay Estuary Watershed In 2008, Philip Trowbridge

PREP Reports & Publications

Nitrogen enrichment is a growing concern for the Great Bay Estuary. For the 2006 State of the Estuaries report (NHEP, 2006), the NHEP calculated the nitrogen load from wastewater treatment facilities (WWTF) using data on total dissolved nitrogen in WWTF effluent in 2002 from Bolster et al. (2003). The NHEP needs to update this indicator for the 2009 State of the Estuaries report. Not only is more recent data needed but also measurements of total nitrogen are needed to avoid assumptions about the ratio of dissolved to total nitrogen. Therefore, the NHEP allocated staff time to collect effluent samples from ...


Isinglass River Conservation Corridor Project, Daniel E. Kern Dec 2008

Isinglass River Conservation Corridor Project, Daniel E. Kern

PREP Reports & Publications

The New Hampshire Estuaries Project (NHEP) provided a $9,075 grant to Bear-Paw Regional Greenways to support the Isinglass River Conservation Corridor Project. The grant helped fund some of the transaction costs associated with the project that protected high-value conservation land along the Isinglass River and in other areas of the watershed in Strafford, New Hampshire. The $2.9 million project was a collaboration of Bear-Paw, the Trust for Public Land (TPL), and the Town of Strafford that protected five properties and 868 acres of forest, over one mile of road frontage, more than three miles of frontage on streams ...


Lake Mead National Recreation Area Vegetation Monitoring And Management: Quarterly Progress Report, October 1, 2008 To December 31, 2008, Margaret N. Rees Dec 2008

Lake Mead National Recreation Area Vegetation Monitoring And Management: Quarterly Progress Report, October 1, 2008 To December 31, 2008, Margaret N. Rees

Vegetation Monitoring

Executive Summary

  • The Weed Sentry program surveyed over 62 miles of federal land by vehicle and foot in Clark County for incipient populations of exotic plants.
  • New weed monitoring botanists and botany assistant joined the staff during this period.
  • Vegetation staff presented four presentations at the Natural Areas Association annual conference in Nashville, TN and eight poster presentations at the “wildfires and invasive plants in American deserts” meeting in Reno, NV.
  • Several new Brassica tournefortii experiments were initiated.


Lake Mead National Recreation Area Sensitive Wildlife Species Monitoring And Analysis: Quarterly Progress Report, Period Ending December 31, 2008, Margaret N. Rees Dec 2008

Lake Mead National Recreation Area Sensitive Wildlife Species Monitoring And Analysis: Quarterly Progress Report, Period Ending December 31, 2008, Margaret N. Rees

Wildlife Monitoring

Project 1. Relict Leopard Frog Monitoring, Management, and Research

  • All milestones and deliverables associated with the MSHCP project are on schedule
  • Completion of 2008 monitoring survey efforts
  • Coordination is ongoing to identify potential translocation sites, including assisting efforts to identify a potential site in the western Grand Canyon
  • RLFCT meeting hosted and minutes of meeting drafted
  • Annual report provided to RLFCT, final draft in review
  • Sampling for the amphibian chytrid fungus conducted at many sites

Project 2. Bald Eagle Winter Monitoring and Evaluation

  • All MSHCP milestones and deliverables are on schedule
  • Planning and coordination of the 2009 Eagle Count conducted ...


Joint Fire Science Program – Lake Mead National Recreation Area: Revegetating Burned Arid Lands: Identifying Successful Native Species Using Trait And Competition Analysis, Quarterly Progress Report, October 1 — December 31, 2008, Margaret N. Rees Dec 2008

Joint Fire Science Program – Lake Mead National Recreation Area: Revegetating Burned Arid Lands: Identifying Successful Native Species Using Trait And Competition Analysis, Quarterly Progress Report, October 1 — December 31, 2008, Margaret N. Rees

Fire Science

• Presented poster at Natural Areas Conference in Nashville, TN

• Initiated granivory study at Goodsprings.

• Outplanted 2376 plants for Experiment 1 at Lake Mead nursery.

• Presented poster at Wildfires and Invasive Plants in American Deserts conference in Reno, NV.

• Submitted manuscript covering our distance from road - microsite invasibility study to Journal of Arid Environments.


Limnological Assistance For The Lake Mead National Recreation Area In Meeting The Challenge Of The Water 2025 Initiative: Quarterly Report, Period Ending December 31, 2008, Margaret N. Rees Dec 2008

Limnological Assistance For The Lake Mead National Recreation Area In Meeting The Challenge Of The Water 2025 Initiative: Quarterly Report, Period Ending December 31, 2008, Margaret N. Rees

Limnological Studies

Project 1: Technical input has been provided at four advisory team meetings attended this quarter.

Project 2: A formal report titled, Surface Water Monitoring for Indicator Bacteria in High-use Sites of the Lake Mead National Recreation Area, has been prepared; an accompanying poster presentation for the Lake Mead Science Symposium is in preparation.

Project 3: A draft document titled, “Interagency Monitoring Action Plan (I-MAP): Quagga Mussels in Lakes Mead and Mohave” has been presented to an interagency core group to review and input. Six oral presentations related to quagga mussels are in preparation by this group for the Lake Mead ...


Rna Secondary Structure Detection Programs With An Emphasis On Covariance Models, Justin Slotman Dec 2008

Rna Secondary Structure Detection Programs With An Emphasis On Covariance Models, Justin Slotman

Theses

RNA secondary structure prediction requires a different approach from traditional alignment methods. Functional RNAs often have their secondary structure better conserved than their primary structure. Covariance models, probabilistic models that utilize stochastic-context-free grammars, are one approach. CMs allow for homology to be detected where purely sequence-based methods would fail. A background on CMs is given, as well as a background of the major classes of non-coding RNAs (ncRNAs). Comparisons are made between some CM-using tools (the Infernal suite and CMfinder) and some other RNA secondary structure tools (CARNAC, miRNAminer, Pfold, Mfold) as well as between Infernal and the primary alignment ...


Integrated Management Strategies For Phytophthora Sojae Combining Host Resistance And Seed Treatments, A. E. Dorrance, A. E. Robertson, S. Cianzo, L. J. Giesler, C. R. Grau, M. A. Draper, A. U. Tenuta, T. R. Anderson Dec 2008

Integrated Management Strategies For Phytophthora Sojae Combining Host Resistance And Seed Treatments, A. E. Dorrance, A. E. Robertson, S. Cianzo, L. J. Giesler, C. R. Grau, M. A. Draper, A. U. Tenuta, T. R. Anderson

Papers in Plant Pathology

Phytophthora sojae has re-emerged as a serious soybean pathogen in the past decade. This may be due in part to changes in resistance levels in current cultivars, adoption of P. sojae populations to deployed Rps genes, and highly favorable environments in the past decade. This multilocation study evaluated the effect of seed treatments on the incidence and severity of Phytophthora root and stem rot on soybeans with different combinations of Rps genes and levels of partial resistance. The efficacy of the seed treatments was highly variable across locations. Seed treatments (metalaxyl and mefenoxam) provided protection and increased yields across cultivars ...


Front Matter, Vol. 68 No. 4 Dec 2008

Front Matter, Vol. 68 No. 4

Western North American Naturalist

No abstract provided.


End Matter, Vol. 68 No. 4 Dec 2008

End Matter, Vol. 68 No. 4

Western North American Naturalist

No abstract provided.


Topographic Effects On The Path And Evolution Of Loop Current Eddies, Kyung Hoon Hyun, Patrick J. Hogan Dec 2008

Topographic Effects On The Path And Evolution Of Loop Current Eddies, Kyung Hoon Hyun, Patrick J. Hogan

Faculty Publications

Eddy-topography (ET) interactions are important in determining the path and evolution of oceanic eddies, including Loop Current Eddies (LCE) in the Gulf of Mexico (GOM). We use the Hybrid Coordinate Ocean Model and satellite altimetry data to investigate the ET interactions and the impact on LCE pathway evolution in the GOM. Satellite altimetry reveals that LCEs translate dominantly westward in the central GOM and strongly collide and reflect against topography near the continental slope in the northern and western GOM. The result is the frequent generation of an anticyclone-cyclone (AC) pair in conjunction with the LCEs. In the absence of ...


The Golgin Gmap210/Trip11 Anchors Ift20 To The Golgi Complex, John A. Follit, Jovenal T. San Agustin, Fenghui Xu, Julie A. Jonassen, Rajeev Samtani, Cecilia W. Lo, Gregory J. Pazour Dec 2008

The Golgin Gmap210/Trip11 Anchors Ift20 To The Golgi Complex, John A. Follit, Jovenal T. San Agustin, Fenghui Xu, Julie A. Jonassen, Rajeev Samtani, Cecilia W. Lo, Gregory J. Pazour

Open Access Articles

Eukaryotic cells often use proteins localized to the ciliary membrane to monitor the extracellular environment. The mechanism by which proteins are sorted, specifically to this subdomain of the plasma membrane, is almost completely unknown. Previously, we showed that the IFT20 subunit of the intraflagellar transport particle is localized to the Golgi complex, in addition to the cilium and centrosome, and hypothesized that the Golgi pool of IFT20 plays a role in sorting proteins to the ciliary membrane. Here, we show that IFT20 is anchored to the Golgi complex by the golgin protein GMAP210/Trip11. Mice lacking GMAP210 die at birth ...


Tax1bp1 Interacts With Papillomavirus E2 And Regulates E2-Dependent Transcription And Stability, Xiaoyu Wang, Samisubbu R. Naidu, Francis Sverdrup, Elliot J. Androphy Dec 2008

Tax1bp1 Interacts With Papillomavirus E2 And Regulates E2-Dependent Transcription And Stability, Xiaoyu Wang, Samisubbu R. Naidu, Francis Sverdrup, Elliot J. Androphy

Open Access Articles

The papillomavirus E2 proteins regulate viral replication, gene transcription, and genome maintenance by interacting with other viral and host proteins. From a yeast two-hybrid screen, we identified the cellular protein Tax1BP1 as a novel binding partner of human papillomavirus type 18 (HPV18) E2. Tax1BP1 also interacts with the HPV16 and bovine papillomavirus type 1 (BPV1) E2 proteins, with the C-terminal region of Tax1BP1 interacting with the N-terminal transactivation domain of BPV1 E2. Tax1BP1 complexes with p300 and acts synergistically as a coactivator with p300 to enhance E2-dependent transcription. Using chromatin immunoprecipitation assays, we show that Tax1BP1 and E2 localize to ...


Structure And Dynamics Of Metalloproteins In Live Cells, Jeremy D. Cook, James E. Penner-Hahn, Timothy L. Stemmler Dec 2008

Structure And Dynamics Of Metalloproteins In Live Cells, Jeremy D. Cook, James E. Penner-Hahn, Timothy L. Stemmler

Biochemistry and Molecular Biology Faculty Publications

X-ray absorption spectroscopy (XAS) has emerged as one of the premier tools for investigating the structure and dynamic properties of metals in cells and in metal containing biomolecules. Utilizing the high flux and broad energy range of X-rays supplied by synchrotron light sources, one can selectively excite core electronic transitions in each metal. Spectroscopic signals from these electronic transitions can be used to dissect the chemical architecture of metals in cells, in cellular components and in biomolecules at varying degrees of structural resolution. With the development of ever-brighter X-ray sources, X-ray methods have grown into applications that can be utilized ...


The Effect Of Oxidant And The Non-Oxidant Alteration Of Cellular Thiol Concentration On The Formation Of Protein Mixed-Disulfides In Hek 293 Cells, Jasen Lee Gilge, Michael Fisher, Yuh-Cherng Chai Dec 2008

The Effect Of Oxidant And The Non-Oxidant Alteration Of Cellular Thiol Concentration On The Formation Of Protein Mixed-Disulfides In Hek 293 Cells, Jasen Lee Gilge, Michael Fisher, Yuh-Cherng Chai

Chemistry

Cellular molecules possess various mechanisms in responding to oxidant stress. In terms of protein responses, protein S-glutathionylation is a unique post-translational modification of protein reactive cysteines forming disulfides with glutathione molecules. This modification has been proposed to play roles in antioxidant, regulatory and signaling in cells under oxidant stress. Recently, the increased level of protein S-glutathionylation has been linked with the development of diseases. In this report, specific S-glutathionylated proteins were demonstrated in human embryonic kidney 293 cells treated with two different oxidative reagents: diamide and hydrogen peroxide. Diamide is a chemical oxidizing agent whereas hydrogen peroxide is a physiological ...


Sex Differences In Mu-Opioid Receptor Expression In The Rat Midbrain Periaqueductal Gray Are Essential For Eliciting Sex Differences In Morphine Analgesia, Dayna R. Loyd, Xioaya Wang, Anne Z. Murphy Phd Dec 2008

Sex Differences In Mu-Opioid Receptor Expression In The Rat Midbrain Periaqueductal Gray Are Essential For Eliciting Sex Differences In Morphine Analgesia, Dayna R. Loyd, Xioaya Wang, Anne Z. Murphy Phd

Neuroscience Institute Faculty Publications

Opioid-based narcotics are the most widely prescribed therapeutic agent for the alleviation of persistent pain; however, it is becoming increasingly clear that morphine is significantly less potent in women compared with men. Morphine primarily binds to mu-opioid receptors (MORs), and the periaqueductal gray (PAG) contains a dense population of MOR-expressing neurons. Via its descending projections to the rostral ventromedial medulla and the dorsal horn of the spinal cord, the PAG is considered an essential neural substrate for opioid-based analgesia. We hypothesized that MOR expression in the PAG was sexually dimorphic, and that these sex differences contribute to the observed sex ...