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Full-Text Articles in Life Sciences

Agrobacterium Mediated Transformation Optimizations For Sugarcane (Saccharum Officinarum L.) Cultivar Spf-234 With Direct Organogenesis, Muhammad Nawaz, Naeem Iqbal, Rabia Hameed, Mehwish Mehwish, Shakra Jamil May 2021

Agrobacterium Mediated Transformation Optimizations For Sugarcane (Saccharum Officinarum L.) Cultivar Spf-234 With Direct Organogenesis, Muhammad Nawaz, Naeem Iqbal, Rabia Hameed, Mehwish Mehwish, Shakra Jamil

Journal of Bioresource Management

Sugarcane (Saccharum officinarum L.) is the most important food and energy crop worldwide. In the present study, an efficient Agrobacterium mediated transformation and regeneration system for sugarcane cultivar SPF-234 was established. Agrobacterium tumefaciens strains EHA101and LBA4404 using vector pIG121 Hm, having GUS, HPTII and NPTII genes were used. Polymerase chain reaction (PCR) and histochemical assays confirmed the GUS gene expression. A 620 bp fragment from GUS positive plants was amplified. The GUS expressing putative transformants were 35% of the total plants formed under 30 minute immersion time and 72 hr of incubation period. The co-cultivation media having 60 µM acetosyringone ...


Responses Of The Carnivorous Pink Spoonleaf Sundew (Drosera Capillaris) To Nitrogen Addition, Phosphorus Addition, And Simulated Fire, Karina Rodriguez Castillo May 2021

Responses Of The Carnivorous Pink Spoonleaf Sundew (Drosera Capillaris) To Nitrogen Addition, Phosphorus Addition, And Simulated Fire, Karina Rodriguez Castillo

Honors Theses

This study investigated how the carnivorous plant, Drosera capillaris(pink sundew), responded to changes in the resources of its habitat, the fire-prone, nutrient-poor wet savannas. It is of interest to determine what soil nutrient(s), nitrogen or phosphorus, limits the survival and growth of pink sundew, and to what extent, if any, does fire affect the survival and growth of this carnivorous plant. My field experiments had twenty plots with two replicate groups consisting of four pink sundews in each plot. From the fall of 2017 to the fall of 2019, only half of the plots were clipped annually, and ...


Evaluating Silicon Foliar Sprays As A Strategy To Improve Postproduction Performance Of Potted Basil (Ocimum Basilicum L.), Joshua Tebow May 2021

Evaluating Silicon Foliar Sprays As A Strategy To Improve Postproduction Performance Of Potted Basil (Ocimum Basilicum L.), Joshua Tebow

Horticulture Undergraduate Honors Theses

The objective of the study was to evaluate foliar silicon (Si) applications for effects on the growth and performance of container-grown basil during production and resistance to postproduction wilt in retail. Basil (Ocimum basilicum ‘Genovese’ L.) seedling plugs were transplanted into 10-cm diameter plastic containers with peat-based substrate and grown for 42 d in a polycarbonate greenhouse. Plants were irrigated with fertilizer solution consisting of a 17.0 nitrogen (N)-1.3 phosphorus-14.1 potassium water-soluble fertilizer dissolved in tap water at 150 mg∙L-1 N. Foliar sprays containing sodium silicate at 0, 50, 100, 200, and 400 mg∙L-1 ...


Not All Pollinator Gardens Are Created Equally: Determining Factors Pertinent To Improving Pollinator Garden Effectiveness, Travis Watson May 2021

Not All Pollinator Gardens Are Created Equally: Determining Factors Pertinent To Improving Pollinator Garden Effectiveness, Travis Watson

Electronic Theses and Dissertations

Increasing evidence documenting the decline of insect populations, resulting from increasing human disturbances has resulted in efforts to establish pollinator gardens to provide additional resources for insect populations. However, our understanding of biotic and abiotic garden characteristics important for attracting and sustaining pollinator diversity is limited. Here, we evaluated 17 pollinator gardens to evaluate the effect of five biotic and three abiotic garden characteristics on pollinator species richness, abundance, and proportional representation of four pollinator functional groups. Plant species richness positively influenced pollinator richness and negatively influenced flower visitation. Bombus proportional abundance responded to several variables (distance to vegetation, plant ...


An Analysis Of The Pollinators Of Echinacea Purpurea In Relation To Their Perceived Efficiency And Color Preferences, Carmen Black May 2021

An Analysis Of The Pollinators Of Echinacea Purpurea In Relation To Their Perceived Efficiency And Color Preferences, Carmen Black

Honors Theses

This study aimed to better understand how insects interacted with species of Echinacea in Tennessee and specifically their preference to floral color. Based on previous studies I expected the main visitors to be composed of various bees, beetles and butterflies. Based on previous studies, I hypothesized that most bees (especially social bees) would most likely pollinate the purple/violet morphs of the flower versus the white morph. I also expected the bees would have the most visits to the coneflowers versus any other taxonomic group. I believe bees to be the majority of pollinators based upon the results of Stucky ...


Three Centuries Of Vegetation Change In The William & Mary College Woods Reconstructed Using Phytoliths, Timothy Terlizzi May 2021

Three Centuries Of Vegetation Change In The William & Mary College Woods Reconstructed Using Phytoliths, Timothy Terlizzi

Undergraduate Honors Theses

The College Woods, west of William & Mary’s campus, consists of ~900 acres of protected southern mixed hardwood forest. The woods surround Lake Matoaka, a former millpond established in ~1700. Despite the rich history of the area, little is known about how the dominant vegetative landcover has shifted over the last 300 years. This study set out to quantify the modern vegetation within the College Woods via the phytolith assemblages within the soil and identify shifts in the assemblages since the creation of Lake Matoaka and whether these changes are distinct from the vegetation that existed in the area before the lake. To accomplish this, I studied the composition and preservation of phytoliths – silica bodies generated within and between plant cells. The study focused on the two questions: do the modern phytolith assemblages in the soil of the College Woods reflect the vegetation present and can phytoliths within the sediments of Lake Matoaka be used to identify the dominant vegetative communities over the last ~300 years? I addressed these questions with three approaches: 1) Identify the primary phytolith producing taxa within the College Woods; 2) Identify the modern phytolith assemblages within the soil of the College Woods; 3) Identify the differences between phytolith assemblages from the lake sediment core samples. I found the following: 1) The production of phytoliths varies heavily between and within different common taxa of the College Woods, with species of oaks (Quercus spp.) unpredictably producing phytoliths and beeches (Fagus grandifolia) likely contributing the majority of dicot phytoliths; 2) The modern phytolith assemblages of the College Woods reflect a low phytolith producing environment, and the vegetative homogeneity is reflected in the phytolith record; 3) The ...


Subtidal Seaweed And Eelgrass Monitoring Photographs 2020, David M. Burdick, Gregg E. Moore Apr 2021

Subtidal Seaweed And Eelgrass Monitoring Photographs 2020, David M. Burdick, Gregg E. Moore

Jackson Estuarine Laboratory

Records of photographs of subtidal seaweed and eelgrass sampling plots for cover (50 by 50 cm) collected by Jackson Estuarine Laboratory research staff in 2020.


Seaweed Monitoring In The Great Bay Estuary, Nh: 2020 Annual Report, Andrew Payne, David M. Burdick, Gregg Moore, Natalie White, Christopher Peter, Arthur Mathieson Apr 2021

Seaweed Monitoring In The Great Bay Estuary, Nh: 2020 Annual Report, Andrew Payne, David M. Burdick, Gregg Moore, Natalie White, Christopher Peter, Arthur Mathieson

Jackson Estuarine Laboratory

As water temperatures rise due to global warming and nitrogen inputs change, it is important to understand how these changes are impacting vegetative communities that form the basic habitat structure in the Great Bay Estuary, NH. The abundance and taxa of intertidal seaweeds have been monitored at fixed locations throughout the Estuary since 2013. In 2020, percent cover and biomass were collected from five intertidal and four subtidal sampling locations. Data from 2013-2020 show appreciable amounts of nuisance seaweeds (primarily reds), including several introduced species. Cover of green seaweeds decreased significantly over time at the two intertidal sites (Depot Road ...


Photographs Of Seaweeds In Intertidal Monitoring Plots, 2020, David M. Burdick, Gregg Moore Apr 2021

Photographs Of Seaweeds In Intertidal Monitoring Plots, 2020, David M. Burdick, Gregg Moore

Jackson Estuarine Laboratory

Records of photographs of intertidal seaweed sampling plots for cover (50 by 50 cm) and biomass (25 by 25 cm) collected by Jackson Estuarine Laboratory research staff in 2020.


Sciadopitys Verticillata (Japanese Umbrella Pine), #1065-1067, Grace C. Kubat Apr 2021

Sciadopitys Verticillata (Japanese Umbrella Pine), #1065-1067, Grace C. Kubat

BIO 140 Arboretum Project

Over the course of the 2021 spring semester, I observed a grove of Japanese Umbrella Pines. These trees are a part of the University's arboretum. In addition to making weekly observations, I researched the cultural significance of the Umbrella Pines. My research will hopefully contribute to future projects regarding the trees and help to enrich the University's arboretum project.


The Phytotherapeutic Arsenal In The Guerbes-Sanhadja Wetlands Complex (North East Of Algeria), Belaidi Abdelouahab, Beghami Yassine, Francisco Maria Vázquez, Souilah Nabila, Bendif Hamdi Apr 2021

The Phytotherapeutic Arsenal In The Guerbes-Sanhadja Wetlands Complex (North East Of Algeria), Belaidi Abdelouahab, Beghami Yassine, Francisco Maria Vázquez, Souilah Nabila, Bendif Hamdi

Journal of Bioresource Management

This study documents indigenous medicinal flora - utilization, and was carried out in the Guerbes-Sanhadja wetland complex, between January 2018 and December 2020. The detailed information was gathered from users of medicinal plants mainly based on semi-structured interviews. A total of 102 plant species distributed in 48 families were found in practice by the natives for fulfilling their daily life requirements. The most frequently cited plants are: Myrtus communis L. (158 citations), Pistacia lentiscus L. (122) and Thymus vulgaris L. (97). Most of the medicinal species listed were herbs (68.93%). The phanerophytes (33.33%) and hemicriptophytes (27.45%) were the ...


Phenotypic Plasticity Of Diverse Sorghum Varieties In Response To Nitrogen Deficit Stress, Mackenzie Zwiener Apr 2021

Phenotypic Plasticity Of Diverse Sorghum Varieties In Response To Nitrogen Deficit Stress, Mackenzie Zwiener

Theses, Dissertations, and Student Research in Agronomy and Horticulture

The movement of nutrients from old vegetative tissue to young leaves and reproductive organs is known as senescence. Senescence can be influenced by the environment of a plant, such as, drought, heat, and nutrient stress. These environmental stressors can also influence canopy architecture. Crop canopy consists of several traits including: leaf number, angle, length and width. Past research in crops like maize and rice have shown significant influences of more erect leaves, including, the ability for increased planting density, which optimizes light interception and increases yield. In a field trial of a sorghum association panel including 345 different sorghum varieties ...


A Baseline Documentation Report For The Delta Wind Birds Sky Lake Nature Reserve, Preston T. Perkins, J Hays Dubberly, Michael J. Thomas Apr 2021

A Baseline Documentation Report For The Delta Wind Birds Sky Lake Nature Reserve, Preston T. Perkins, J Hays Dubberly, Michael J. Thomas

Honors Theses

The purpose of this baseline documentation is to conduct a report on our subject property and discuss the findings. A conservation easement is established by upholding a certain standard of a property’s current condition which makes a baseline documentation necessary for an easement to be enacted. Baseline documentation reports are used as a resource to monitor and enforce the legal agreements of the conservation easement. The subject property is currently owned by the Delta Wind Birds organization. In our scenario, this documentation report will serve to aid in the conversion of our subject property into a conservation easement in ...


Tilia Cordata (Littleleaf Linden) #1059, Heather Noel Mckee Apr 2021

Tilia Cordata (Littleleaf Linden) #1059, Heather Noel Mckee

BIO 140 Arboretum Project

In this paper I studied the Tilia cordata, more commonly known as the Littleleaf Linden, over the course of three months. I included the history and uses of the Linden tree along with its symbolic value in European culture. This tree symbolizes friendship and peace which coincides with the critical concerns of the Sisters of Mercy.


The Influence Of Adjuvants On Physical Properties, Droplet-Size, And Efficacy Of Glufosinate And Dicamba Plus Glyphosate Solutions, Estefania Gomiero Polli Apr 2021

The Influence Of Adjuvants On Physical Properties, Droplet-Size, And Efficacy Of Glufosinate And Dicamba Plus Glyphosate Solutions, Estefania Gomiero Polli

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Adjuvants are used in agriculture to improve herbicide activity or application performance. The addition of adjuvants to herbicide solution can enhance its penetration, wettability, and evaporation rates by altering density, viscosity, contact angle between the droplet and plant surface, and droplet surface tension. Furthermore, those alterations in the physical properties of the herbicide solution can result in changes in the droplet-size distribution that directly impact herbicide efficacy. The adoption of glufosinate-based herbicide programs has increased with the widespread occurrence of glyphosate-resistance (GR) weeds in recent years. Also, tank mixture of dicamba and glyphosate has been largely adopted for broad-spectrum weed ...


Seed Rain–Successional Feedbacks In Wet Tropical Forests, Nohemi Huanca Nuñez, Robin L. Chazdon, Sabrina E. Russo Apr 2021

Seed Rain–Successional Feedbacks In Wet Tropical Forests, Nohemi Huanca Nuñez, Robin L. Chazdon, Sabrina E. Russo

Faculty Publications in the Biological Sciences

Abstract

Tropical forest regeneration after abandonment of former agricultural land depends critically on the input of tree seeds, yet seed dispersal is increasingly disrupted in contemporary human-modified landscapes. Here, we introduce the concept of seed rain–successional feedbacks as a deterministic process in which seed rain is shaped by successional dynamics internal to a forest site and that acts to reinforce priority effects. We used a combination of time series and chronosequence approaches to investigate how the quantity and taxonomic and functional composition of seed rain change during succession and to evaluate the strength of seed rain–successional feedbacks, relative ...


Maize Nitrogen Management Using Reactive Sensor And Proactive Maize-N Model Via Fertigation, Mohammed A. Naser Apr 2021

Maize Nitrogen Management Using Reactive Sensor And Proactive Maize-N Model Via Fertigation, Mohammed A. Naser

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Applying a portion of total nitrogen (N) during the growing season has the potential to improve nitrogen use efficiency (NUE) by achieving greater synchrony between N supply and crop N demand, allowing for responsive adjustments to actual field conditions. Three studies from 2017-2019 evaluated using reactive sensor and proactive Maize-N model for determining in-season N requirements via fertigation in corn. The first study evaluated the integration of reactive sensor and proactive Maize-N model for determining the timing and rate of in-season N via fertigation. Overall, reactive and proactive fertigation treatments reduced total N applied by 35 to 65 kg N ...


Soil Erosion Modelling: A Bibliometric Analysis, Nejc Bezak, Matjǎz Mikǒs, Pasquale Borrelli, Christine Alewell, Dinesh Panday, Panos Panagos, 59 Co-Authors Mar 2021

Soil Erosion Modelling: A Bibliometric Analysis, Nejc Bezak, Matjǎz Mikǒs, Pasquale Borrelli, Christine Alewell, Dinesh Panday, Panos Panagos, 59 Co-Authors

Agronomy & Horticulture -- Faculty Publications

Soil erosion can present a major threat to agriculture due to loss of soil, nutrients, and organic carbon. Therefore, soil erosion modelling is one of the steps used to plan suitable soil protection measures and detect erosion hotspots. A bibliometric analysis of this topic can reveal research patterns and soil erosion modelling characteristics that can help identify steps needed to enhance the research conducted in this field. Therefore, a detailed bibliometric analysis, including investigation of collaboration networks and citation patterns, should be conducted. The updated version of the Global Applications of Soil Erosion Modelling Tracker (GASEMT) database contains information about ...


Project Home Hydroponic Operations For Mars Exploration, Deanna Demattio, Nick Mcguire, Ruben A. Rosa Polonia, Benjamin T. Hufendick Mar 2021

Project Home Hydroponic Operations For Mars Exploration, Deanna Demattio, Nick Mcguire, Ruben A. Rosa Polonia, Benjamin T. Hufendick

Beyond: Undergraduate Research Journal

This report considers the challenges NASA, SpaceX, and other private companies will face in the approaching two decades when sending astronauts on missions to Mars. The longest exploration is planned to take place in the 2030's, sending a crew of, at minimum, four astronauts to Mars for a year of research. The research conducted is assisting space exploration companies’ with ways to grow a complete diet on a planet that does not receive enough sunlight. Agriculture in enclosed and buried structures on Mars will enable astronauts to conduct extended surface exploration missions. We evaluated a deep-water culture indoor hydroponics ...


Wayne E. Sabbe Arkansas Soil Fertility Studies 2020, Nathan A. Slaton Mar 2021

Wayne E. Sabbe Arkansas Soil Fertility Studies 2020, Nathan A. Slaton

Arkansas Agricultural Experiment Station Research Series

Rapid technological changes in crop management and production require that the research efforts be presented in an expeditious manner. The contributions of soil fertility and fertilizers are major production factors in all Arkansas crops. The studies described within will allow producers to compare their practices with the university’s research efforts. Additionally, soil-test data and fertilizer sales are presented to allow comparisons among years, crops, and other areas within Arkansas.


Ascorbic Acid (Asa) Improves Salinity Tolerance In Wheat (Triticum Aestivum L.) By Modulating Growth And Physiological Attributes, Hasnain Ishaq, Muhammad Nawaz, Muhammad Azeem, Mehwish Mehwish, Muhammad Bedar Bekhat Naseem Jan 2021

Ascorbic Acid (Asa) Improves Salinity Tolerance In Wheat (Triticum Aestivum L.) By Modulating Growth And Physiological Attributes, Hasnain Ishaq, Muhammad Nawaz, Muhammad Azeem, Mehwish Mehwish, Muhammad Bedar Bekhat Naseem

Journal of Bioresource Management

Wheat (Triticum aestivum L.) is one of the most important staple foods. High concentration of sodium chloride severely affects plants in general and wheat in specific. In this study the ameliorative effects of ascorbic acid (AsA) against sodium chloride stress were investigated in two commercial wheat cultivars (Galaxy 2013 and Akbar 2019). Experiments were conducted in three replicates. Two levels of salt (0, 150 mM) along with exogenous application of ascorbic acid (0, 30 mM, 60 mM) were applied at three leaves seedling stage. At the establishment of treatments, data regarding physiological, biochemical and yield attributes were recorded and subjected ...


Diversity, Ecology And Therapeutic Properties Of The Medicinal Plants In Ziban Region (Algeria), Nouidjem Yassine, Hadjab Ramzi, Khammar Hichem, Merouani Sakina, Bensaci Etayeb Jan 2021

Diversity, Ecology And Therapeutic Properties Of The Medicinal Plants In Ziban Region (Algeria), Nouidjem Yassine, Hadjab Ramzi, Khammar Hichem, Merouani Sakina, Bensaci Etayeb

Journal of Bioresource Management

With the aim to assess the floristic population diversity, distribution and therapeutic properties in the Ziban region (Algeria), a survey was conducted along two successive study years in the main area. The objective of this study was to contribute to the knowledge and description of this plant groups with exhaustive inventory spontaneous vegetation. In order to study the dynamics of medicinal plants, we used a quantitative method based essentially on the classic technique of linear surveys and floristic inventory using the minimum area method. The results showed that the floristic procession of medicinal plants is represented by 21 species belonging ...


Soil Properties Limiting Vegetation Establishment Along Roadsides, Shad D. Mills, Martha Mamo, Sabrina J. Ruis, Humberto Blanco-Canqui, Walter Schacht, Tala Awada, Xu Li, Pamela Sutton Jan 2021

Soil Properties Limiting Vegetation Establishment Along Roadsides, Shad D. Mills, Martha Mamo, Sabrina J. Ruis, Humberto Blanco-Canqui, Walter Schacht, Tala Awada, Xu Li, Pamela Sutton

Agronomy & Horticulture -- Faculty Publications

Roadside vegetation provides a multitude of ecosystem services, including pollutant remediation, runoff reduction, wildlife habitat, and aesthetic scenery. Establishment of permanent vegetation along paved roads after construction can be challenging, particularly within 1 m of the pavement. Adverse soil conditions could be one of the leading factors limiting roadside vegetation growth. In this study, we assessed soil physical and chemical properties along a transect perpendicular to the road at six microtopographic positions (road edge, shoulder, side slope, ditch, backslope, and field edge) along two highway segments near Beaver Crossing and Sargent, NE. At the Beaver Crossing site, Na concentration was ...


Environmental Data: Geospatial And Elevation Data, Albert J. Burky, Julia I. Chapman, Ryan W. Mcewan Jan 2021

Environmental Data: Geospatial And Elevation Data, Albert J. Burky, Julia I. Chapman, Ryan W. Mcewan

Data Files: Drew Woods Permanent Plots

GPS Coordinates, Elevation, and Distance to Edge data for 32 sample plots in Drew Woods State Nature Preserve in Darke County, OH. Data key is provided in a supplemental file.


A Comparison Of Media To Determine Optimal Growth In Aquaponics: Prg Virtual Showcase January 2021, Rachel L. Fogle, Andrea Nagy, Claribel Asare, Steven Berry, Jordan Brown, Aquila Durham-Lewis Jan 2021

A Comparison Of Media To Determine Optimal Growth In Aquaponics: Prg Virtual Showcase January 2021, Rachel L. Fogle, Andrea Nagy, Claribel Asare, Steven Berry, Jordan Brown, Aquila Durham-Lewis

Presidential Research Grants

No abstract provided.


Does Biochar Improve All Soil Ecosystem Services?, Humberto Blanco-Canqui Jan 2021

Does Biochar Improve All Soil Ecosystem Services?, Humberto Blanco-Canqui

Agronomy & Horticulture -- Faculty Publications

Biochar is considered to sequester C and deliver other soil ecosystem services, but an overview that synthesizes the current knowledge of biochar implications on all essential soil ecosystem services is difficult to find in the ample biochar literature. Most previous research and review articles on this topic focused on a single ecosystem service and did not integrate all essential soil ecosystem services. This overview paper (1) synthesizes the impacts of biochar on water and wind erosion, C sequestration, soil water, nutrient leaching, soil fertility, crop yields, and other soil ecosystem services based on published literature and (2) highlights remaining research ...


James B Beard: The Father Of Contemporary Turfgrass Science, G.K. Stahnke, P.E. Rieke, Robert C. Shearman, Roch E. Gaussoin, David Kopec Jan 2021

James B Beard: The Father Of Contemporary Turfgrass Science, G.K. Stahnke, P.E. Rieke, Robert C. Shearman, Roch E. Gaussoin, David Kopec

Agronomy & Horticulture -- Faculty Publications

James B Beard (24 Sept 1935 to 8 May 2018) can rightly be considered the “Father” of contemporary turfgrass science. During his career, he was known for setting a standard that provided the foundation for turfgrass science through his thorough approach to research, teaching, mentoring and communications. The books he published outlined a vision for an evolving scientific discipline. He trained and mentored >45 domestic and international doctoral and master students and numerous post-doctoral trainees, who in turn continued to raise the quality of contemporary turfgrass science in the USA and internationally. He led the effort to change the name ...


Intervention Of Climate-Smart Practices In Wheat Under Rice-Wheat Cropping System In Nepal, Janma Jaya Gairhe, Mandeep Adhikari, Deepak Ghimire, Arun Khatri-Chhetri, Dinesh Panday Jan 2021

Intervention Of Climate-Smart Practices In Wheat Under Rice-Wheat Cropping System In Nepal, Janma Jaya Gairhe, Mandeep Adhikari, Deepak Ghimire, Arun Khatri-Chhetri, Dinesh Panday

Agronomy & Horticulture -- Faculty Publications

Besides a proper agronomic management followed by Nepalese farmers, wheat (Triticum aestivum L.) production has been severely affected by changing climate. There are many interventions, including climate-smart practices, to cope with this situation and possibly enhance crop and soil productivity. Field experiments were set up in a randomized complete block design with six treatments (TRT) with four replications in three locations (LOC) during wheat-growing seasons in Nepal from 2014 to 2016. Treatments included (i) Controlled Practice (CP), (ii) Improved Low (IL), (iii) Improved High (IH), (iv) Climate Smart Agriculture Low (CSAL), (v) Climate Smart Agriculture Medium (CSAM), and (vi) Climate ...


Development Of Mping-Based Activation Tags For Crop Insertional Mutagenesis, Alexander Johnson, Edward Mcassey, Stephanie Diaz, Jacod Reagin, Priscilla S. Redd, Daymond R. Parrilla, Hanh Nguyen, Adrian Stec, Lauren A.L. Mcdaniel, Thomas E. Clemente, Robert M. Stupar, Wayne A. Parrott, C. Nathan Hancock Jan 2021

Development Of Mping-Based Activation Tags For Crop Insertional Mutagenesis, Alexander Johnson, Edward Mcassey, Stephanie Diaz, Jacod Reagin, Priscilla S. Redd, Daymond R. Parrilla, Hanh Nguyen, Adrian Stec, Lauren A.L. Mcdaniel, Thomas E. Clemente, Robert M. Stupar, Wayne A. Parrott, C. Nathan Hancock

Agronomy & Horticulture -- Faculty Publications

Modern plant breeding increasingly relies on genomic information to guide crop im- provement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to in- duce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing-based activation tags and the appropriate transposase expression cassettes showed evidence of transposition ...


Responses Of Soil Surface Greenhouse Gas Emissions To Nitrogen And Sulfur Fertilizer Rates To Brassica Carinata Grown As A Bio-Jet Fuel, Dwarika Bhattarai, Gandura O. Abagandura, Thandiwe Nleya, Sandeep Kumar Jan 2021

Responses Of Soil Surface Greenhouse Gas Emissions To Nitrogen And Sulfur Fertilizer Rates To Brassica Carinata Grown As A Bio-Jet Fuel, Dwarika Bhattarai, Gandura O. Abagandura, Thandiwe Nleya, Sandeep Kumar

Agronomy & Horticulture -- Faculty Publications

Carinata (Brassica carinata A. Braun), a non-food oilseed crop and an alternative bio-jet fuel feedstock, has received attention for its potential as a low-input option for production in the semi-arid region of the Northern Great Plains of the United States. Research addressing the impacts of nitrogen (N) and sulfur (S) fertilizers on soils and greenhouse gas (GHG; CO2, N2O, and CH4) emissions from carinata production are limited. Thus, objective of this study was to evaluate the impact of different rates of N and S fertilizers applied to carinata on soil properties and GHG emissions. Field experiments were conducted in 2017 ...