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Faculty Publications

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Articles 1 - 30 of 1979

Full-Text Articles in Life Sciences

Environmental Rnai Pathways In The Two-Spotted Spider Mite, Mosharrof Mondal, Jacob Peter, Obrie Scarbrough, Alex Flynt Dec 2021

Environmental Rnai Pathways In The Two-Spotted Spider Mite, Mosharrof Mondal, Jacob Peter, Obrie Scarbrough, Alex Flynt

Faculty Publications

© 2020, The Author(s).

Background:RNA interference (RNAi) regulates gene expression in most multicellular organisms through binding of small RNA effectors to target transcripts. Exploiting this process is a popular strategy for genetic manipulation and has applications that includes arthropod pest control. RNAi technologies are dependent on delivery method with the most convenient likely being feeding, which is effective in some animals while others are insensitive. The two-spotted spider mite, Tetranychus urticae, is prime candidate for developing RNAi approaches due to frequent occurrence of conventional pesticide resistance. Using a sequencing-based approach, the fate of ingested RNAs was explored to identify ...


Advance Quantity Meal Preparation Pilot Program Improves Home-Cooked Meal Consumption, Cooking Attitudes, And Self-Efficacy, Shannon Mendez, Jamie Kubota, Adrianne Widaman, John Gieng Feb 2021

Advance Quantity Meal Preparation Pilot Program Improves Home-Cooked Meal Consumption, Cooking Attitudes, And Self-Efficacy, Shannon Mendez, Jamie Kubota, Adrianne Widaman, John Gieng

Faculty Publications

Objective

To evaluate the effects of a group-based Advance Quantity Meal Preparation (AQMP) program on the consumption of home-cooked meals, cooking attitudes, and self-efficacy in healthy adults.

Methods

Participants (n = 10) in a group setting prepared healthy meals weekly consisting of 10 entrees and 5 snacks for 6 weeks. A survey assessing cooking attitudes, cooking self-efficacy, and cooking behavior and consumption at 3 time points: preprogram, postprogram (T2), and 3 months postprogram (T3).

Results

The AQMP program increased the proportion of overall home-cooked meal consumption (T2, P = 0.03), home-cooked dinner consumption (T2, P = 0.04), cooking attitudes (T3, P ...


Payment For Ecosystem Services: Rewarding The Landowner Who Conserves The Public Good, Steven H. Bullard, Sarah Fuller Jan 2021

Payment For Ecosystem Services: Rewarding The Landowner Who Conserves The Public Good, Steven H. Bullard, Sarah Fuller

Faculty Publications

It has been said that money doesn’t grow on trees, but any forest landowner or manager will tell you that’s not exactly true— especially when observing a harvesting operation or managing dues from your hunting lease. While timber production and recreation are the most frequently monetized services provided by forests, what about the other goods and services they provide on a continuing basis? Are you or other forest landowners in your area being monetarily rewarded for soil stability, flood control, water filtration, air quality, and the other critical services—known as ecosystem services—provided by forests?


Propranolol Sensitizes Vascular Sarcoma Cells To Doxorubicin By Altering Lysosomal Drug Sequestration And Drug Efflux, Jhuma Saha, Jong Hyuk Kim, Clarissa N. Amaya, Caleb M. Witcher, Ali Khammanivong, Derek M. Korpela, David R. Brown, Josephine Taylor, Brad A. Bryan, Erin B. Dickerson Jan 2021

Propranolol Sensitizes Vascular Sarcoma Cells To Doxorubicin By Altering Lysosomal Drug Sequestration And Drug Efflux, Jhuma Saha, Jong Hyuk Kim, Clarissa N. Amaya, Caleb M. Witcher, Ali Khammanivong, Derek M. Korpela, David R. Brown, Josephine Taylor, Brad A. Bryan, Erin B. Dickerson

Faculty Publications

Angiosarcoma is a rare cancer of blood vessel–forming cells with a high patient mortality and few treatment options. Although chemotherapy often produces initial clinical responses, outcomes remain poor, largely due to the development of drug resistance. We previously identified a subset of doxorubicin-resistant cells in human angiosarcoma and canine hemangiosarcoma cell lines that exhibit high lysosomal accumulation of doxorubicin. Hydrophobic, weak base chemotherapeutics, like doxorubicin, are known to sequester within lysosomes, promoting resistance by limiting drug accessibility to cellular targets. Drug synergy between the beta adrenergic receptor (β-AR) antagonist, propranolol, and multiple chemotherapeutics has been documented in vitro, and ...


Measuring Building Height Using Point Cloud Data Derived From Unmanned Aerial System Imagery In An Undergraduate Geospatial Science Course, David L. Kulhavy, I-Kuai Hung, Daniel R. Unger, Reid Viegut, Yanli Zhang Jan 2021

Measuring Building Height Using Point Cloud Data Derived From Unmanned Aerial System Imagery In An Undergraduate Geospatial Science Course, David L. Kulhavy, I-Kuai Hung, Daniel R. Unger, Reid Viegut, Yanli Zhang

Faculty Publications

The use of Unmanned Aerial Systems (UAS), also known as drones is increasing in geospatial science curricula within the United States. Within the Arthur Temple College of Forestry and Agriculture (ATCOFA) at Stephen F. Austin State University, Texas, seniors in the geospatial science program complete capstone projects to evaluate current geospatial technology to investigate complex ecological, social and environmental issues. Under the umbrella of a student initiated and designed senior project, students designed a study to estimate height of buildings with UAS data incorporating UAS data, LP360 and ArcScene programs, and Pictometry web-based interface. Results from a statistical analysis of ...


Identification And Functional Analysis Of Thadh1 And Thadh4 Genes Involved In Tolerance To Waterlogging Stress In Taxodium Hybrid ‘Zhongshanshan 406’, Lei Xuan, Jianfeng Hua, Fan Zhang, Zhiquan Wang, Xiaoxiao Pei, Ying Yang, Yunlong Yin, David Creech Jan 2021

Identification And Functional Analysis Of Thadh1 And Thadh4 Genes Involved In Tolerance To Waterlogging Stress In Taxodium Hybrid ‘Zhongshanshan 406’, Lei Xuan, Jianfeng Hua, Fan Zhang, Zhiquan Wang, Xiaoxiao Pei, Ying Yang, Yunlong Yin, David Creech

Faculty Publications

The Taxodium hybrid ‘Zhongshanshan 406’ (T. hybrid ‘Zhongshanshan 406’) [Taxodium mucronatum Tenore × Taxodium distichum (L.). Rich] has an outstanding advantage in flooding tolerance and thus has been widely used in wetland afforestation in China. Alcohol dehydrogenase genes (ADHs) played key roles in ethanol metabolism to maintain energy supply for plants in low-oxygen conditions. Two ADH genes were isolated and characterized—ThADH1 and ThADH4 (GenBank ID: AWL83216 and AWL83217—basing on the transcriptome data of T. hybrid ‘Zhongshanshan 406’ grown under waterlogging stress. Then the functions of these two genes were investigated through transient expression and overexpression. The results showed that ...


Swimming Against The Flow: Environmental Dna Can Detect Bull Sharks (Carcharhinus Leucas) Across A Dynamic Deltaic Interface, James Marcus Drymon, Katherine E. Schweiss, Emily A. Seubert, Ryan N. Lehman, Toby S. Daly-Engel, Mariah Pfleger, Nicole M. Phillips Jan 2021

Swimming Against The Flow: Environmental Dna Can Detect Bull Sharks (Carcharhinus Leucas) Across A Dynamic Deltaic Interface, James Marcus Drymon, Katherine E. Schweiss, Emily A. Seubert, Ryan N. Lehman, Toby S. Daly-Engel, Mariah Pfleger, Nicole M. Phillips

Faculty Publications

© 2020 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd Human activities in coastal areas are accelerating ecosystem changes at an unprecedented pace, resulting in habitat loss, hydrological modifications, and predatory species declines. Understanding how these changes potentially cascade across marine and freshwater ecosystems requires knowing how mobile euryhaline species link these seemingly disparate systems. As upper trophic level predators, bull sharks (Carcharhinus leucas) play a crucial role in marine and freshwater ecosystem health. Telemetry studies in Mobile Bay, Alabama, suggest that bull sharks extensively use the northern portions of the bay, an estuarine–freshwater interface known as ...


Hurricane Irma Induces Divergent Behavioral And Hormonal Impacts On An Urban And Forest Population Of Invasive Anolis Lizards: Evidence For An Urban Resilience Hypothesis, Haralambos Fokidis, Taylor Brock Jan 2021

Hurricane Irma Induces Divergent Behavioral And Hormonal Impacts On An Urban And Forest Population Of Invasive Anolis Lizards: Evidence For An Urban Resilience Hypothesis, Haralambos Fokidis, Taylor Brock

Faculty Publications

Hurricanes can have both profound short-term effects on animal populations and serve as long-term drivers of evolutionary change. Animals inhabiting varying habitats may differ in their response to hurricane impacts. Increasing evidence sug- gests that animals from urban areas exhibit different behavioral and physiological traits compared to rural counterparts, in- cluding attenuated hormonal stress responses and a lowered propensity for flight behavior. A unique opportunity was pre- sented when Hurricane Irma hit Florida on 10 September 2017 and interrupted a study of invasive brown anoles (Anolis sagrei) at an urban and a forest. Using data collected before and after Hurricane ...


Two Hundred And Fifty-Four Metagenome-Assembled Bacterial Genomes From The Bank Vole Gut Microbiota, Anton Lavrinienko, Eugene Tukalenko, Timothy A. Mousseau, Luke R. Thompson, Rob Knight, Tapio Mappes, Phillip C. Watts Dec 2020

Two Hundred And Fifty-Four Metagenome-Assembled Bacterial Genomes From The Bank Vole Gut Microbiota, Anton Lavrinienko, Eugene Tukalenko, Timothy A. Mousseau, Luke R. Thompson, Rob Knight, Tapio Mappes, Phillip C. Watts

Faculty Publications

Vertebrate gut microbiota provide many essential services to their host. To better understand the diversity of such services provided by gut microbiota in wild rodents, we assembled metagenome shotgun sequence data from a small mammal, the bank vole Myodes glareolus (Rodentia, Cricetidae). We were able to identify 254 metagenome assembled genomes (MAGs) that were at least 50% (n = 133 MAGs), 80% (n = 77 MAGs) or 95% (n = 44 MAGs) complete. As typical for a rodent gut microbiota, these MAGs are dominated by taxa assigned to the phyla Bacteroidetes (n = 132 MAGs) and Firmicutes (n = 80), with some Spirochaetes (n = 15 ...


Biochemical And Genetic Analysis Of Ecm14, A Conserved Fungal Pseudopeptidase, R. Christian Mcdonald, Matthew James Schott, Temitope A. Idowu, Peter J. Lyons Dec 2020

Biochemical And Genetic Analysis Of Ecm14, A Conserved Fungal Pseudopeptidase, R. Christian Mcdonald, Matthew James Schott, Temitope A. Idowu, Peter J. Lyons

Faculty Publications

© 2020, The Author(s). Background: Like most major enzyme families, the M14 family of metallocarboxypeptidases (MCPs) contains a number of pseudoenzymes predicted to lack enzyme activity and with poorly characterized molecular function. The genome of the yeast Saccharomyces cerevisiae encodes one member of the M14 MCP family, a pseudoenzyme named Ecm14 proposed to function in the extracellular matrix. In order to better understand the function of such pseudoenzymes, we studied the structure and function of Ecm14 in S. cerevisiae. Results: A phylogenetic analysis of Ecm14 in fungi found it to be conserved throughout the ascomycete phylum, with a group of ...


Structure–Activity Relationship-Based Chemical Classification Of Highly Imbalanced Tox21 Datasets, Gabriel Idakwo, Sundar Thangapandian, Joseph Luttrell, Yan Li, Nan Wang, Zhaoxian Zhou, Huixiao Hong, Bei Yang, Chaoyang Zhang, Ping Gong Dec 2020

Structure–Activity Relationship-Based Chemical Classification Of Highly Imbalanced Tox21 Datasets, Gabriel Idakwo, Sundar Thangapandian, Joseph Luttrell, Yan Li, Nan Wang, Zhaoxian Zhou, Huixiao Hong, Bei Yang, Chaoyang Zhang, Ping Gong

Faculty Publications

The specificity of toxicant-target biomolecule interactions lends to the very imbalanced nature of many toxicity datasets, causing poor performance in Structure–Activity Relationship (SAR)-based chemical classification. Undersampling and oversampling are representative techniques for handling such an imbalance challenge. However, removing inactive chemical compound instances from the majority class using an undersampling technique can result in information loss, whereas increasing active toxicant instances in the minority class by interpolation tends to introduce artificial minority instances that often cross into the majority class space, giving rise to class overlapping and a higher false prediction rate. In this study, in order to ...


The Role Of The Msaabcr Operon In Implant-Associated Chronic Osteomyelitis In Staphylococcus Aureus Usa300 Lac, Gyan S. Sahukhal, Michelle Tucci, Hamed Benghuzzi, Gerri Wilson, Mohamed O. Elasri Dec 2020

The Role Of The Msaabcr Operon In Implant-Associated Chronic Osteomyelitis In Staphylococcus Aureus Usa300 Lac, Gyan S. Sahukhal, Michelle Tucci, Hamed Benghuzzi, Gerri Wilson, Mohamed O. Elasri

Faculty Publications

The msaABCR operon regulates several staphylococcal phenotypes such as biofilm formation, capsule production, protease production, pigmentation, antibiotic resistance, and persister cells formation. The msaABCR operon is required for maintaining the cell wall integrity via affecting peptidoglycan cross-linking. The msaABCR operon also plays a role in oxidative stress defense mechanism, which is required to facilitate persistent and recurrent staphylococcal infections. Staphylococcus aureus is the most frequent cause of chronic implant-associated osteomyelitis (OM). The CA-MRSA USA300 strains are predominant in the United States and cause severe infections, including bone and joint infections.


Transport Of N-Cd And Pre-Sorbed Pb In Saturated Porous Media, Salahaddin Kamrani, Vahab Amiri, Mosleh Kamrani, Mohammed Baalousha Nov 2020

Transport Of N-Cd And Pre-Sorbed Pb In Saturated Porous Media, Salahaddin Kamrani, Vahab Amiri, Mosleh Kamrani, Mohammed Baalousha

Faculty Publications

Carbon dots (CDs) are a new type of nanomaterials of the carbon family with unique characteristics, such as their small size (e.g., <10 nm), high water solubility, low toxicity, and high metal affinity. Modification of CDs by Nitrogen functional groups (N-CDs) enhances their metal adsorption capacity. This study investigated the influences of pH (4, 6, and 9), ionic strength (1, 50, and 100 mM), and cation valency (Na+ and Ca2+) on the competitive adsorption of Pb to quartz and N-CD surfaces, the transport and retention of N-CDs in saturated porous media, and the capacity of N-CDs to mobilize pre-adsorbed Pb in quartz columns. Pb adsorption was higher on N-CDs than on quartz surfaces and decreased with increases in ionic strength (IS) and divalent cations (Ca2+) concentration. N-CD mobility in quartz columns was highest at pH of 9- and 1-mM monovalent cations (Na+) and ...


Inferring Life History Characteristics Of The Oceanic Whitetip Shark Carcharhinus Longimanus From Vertebral Bomb Radiocarbon, Michelle S. Passerotti, Allen H. Andrews, Lisa J. Natanson Nov 2020

Inferring Life History Characteristics Of The Oceanic Whitetip Shark Carcharhinus Longimanus From Vertebral Bomb Radiocarbon, Michelle S. Passerotti, Allen H. Andrews, Lisa J. Natanson

Faculty Publications

Oceanic whitetip sharks Carcharhinus longimanus are a cosmopolitan epipelagic species that was once prolific throughout the tropics and subtropics but was recently listed as Critically Endangered by the International Union for the Conservation of Nature and as Threatened under the United States Endangered Species Act. Although historically conspicuous in oceanic fisheries catches, relatively little is known about their habitat use, movement, and life history during migration. Given the paucity of data on migratory patterns and lack of age estimate validation available for this species, we evaluated vertebral growth bands for bomb radiocarbon (14C) patterns to derive additional information on these ...


A Novel Mutation Of The Narrow Leaf 1 Gene Adversely Affects Plant Architecture In Rice (Oryza Sativa L.), Prasanta K. Subudhi, Richard S. Garcia, Sapphire Coronejo, Teresa B. De Leon Nov 2020

A Novel Mutation Of The Narrow Leaf 1 Gene Adversely Affects Plant Architecture In Rice (Oryza Sativa L.), Prasanta K. Subudhi, Richard S. Garcia, Sapphire Coronejo, Teresa B. De Leon

Faculty Publications

Plant architecture is critical for enhancing the adaptability and productivity of crop plants. Mutants with an altered plant architecture allow researchers to elucidate the genetic network and the underlying mechanisms. In this study, we characterized a novel nal1 rice mutant with short height, small panicle, and narrow and thick deep green leaves that was identified from a cross between a rice cultivar and a weedy rice accession. Bulked segregant analysis coupled with genome re-sequencing and cosegregation analysis revealed that the overall mutant phenotype was caused by a 1395-bp deletion spanning over the last two exons including the transcriptional end site ...


Microbiological, Physicochemical, And Immunological Analysis Of A Commercial Cashew Nut-Based Yogurt, Christopher P. Mattison, Kayanush Aryana, Kristen Clermont, Eric Prestenburg, Steven W. Lloyd, Casey .. Grimm, Richard L. Wasserman Nov 2020

Microbiological, Physicochemical, And Immunological Analysis Of A Commercial Cashew Nut-Based Yogurt, Christopher P. Mattison, Kayanush Aryana, Kristen Clermont, Eric Prestenburg, Steven W. Lloyd, Casey .. Grimm, Richard L. Wasserman

Faculty Publications

Nut-based milks and yogurts are gaining popularity, but may not offer the same benefits as dairy yogurts to consumers. Cashew nuts often cause severe allergic reactions, and cashew nut allergens are stable to several types of processing. To compare its characteristics to dairy yogurt and characterize the effects of fermentation on the Ana o 1-3 cashew nut allergens, a commercial yogurt made from cashew nuts (Cashewgurt) was evaluated for microbiological, physiochemical, and immunological properties. Average counts for lactobacilli and Streptococcus thermophilus were greater than 10 million colony forming units per milliliter, indicating the capacity to provide a health benefit. Cashewgurt ...


Speciation-By-Depth On Coral Reefs: Sympatric Divergence With Gene Flow Or Cryptic Transient Isolation?, Carlos Prada, Michael E. Hellberg Nov 2020

Speciation-By-Depth On Coral Reefs: Sympatric Divergence With Gene Flow Or Cryptic Transient Isolation?, Carlos Prada, Michael E. Hellberg

Faculty Publications

The distributions of many sister species in the sea overlap geographically but are partitioned along depth gradients. The genetic changes leading to depth segregation may evolve in geographic isolation as a prerequisite to coexistence or may emerge during primary divergence leading to new species. These alternatives can now be distinguished via the power endowed by the thousands of scorable loci provided by second-generation sequence data. Here, we revisit the case of two depth-segregated, genetically isolated ecotypes of the nominal Caribbean candelabrum coral Eunicea flexuosa. Previous analyses based on a handful of markers could not distinguish between models of genetic exchange ...


Hydroxychloroquine Prophylaxis For Covid-19, David D. Busath Oct 2020

Hydroxychloroquine Prophylaxis For Covid-19, David D. Busath

Faculty Publications

It is common in medicine that simple, inexpensive remedies are held hostage to medical prejudice, financial or political interest, and legal precedent. The purpose of this review is to take the point of view of the victims of COVID-19 and address whether scientific information, including randomized-control trials, can answer whether the hydroxychloroquine should be dispensed for those testing positive and their immediate recent contacts at the point of testing. Although the FDA in the United States is yet to be convinced, the demonstration of positive effects in clinical trials cannot be ignored and there is a large amount of information ...


Use Of Altmetrics To Analyze Scholarworks In Natural Resource Management, David L. Kulhavy, R Philip Reynolds, Daniel R. Unger, Matthew W. Mcbroom, I-Kuai Hung, Yanli Zhang Oct 2020

Use Of Altmetrics To Analyze Scholarworks In Natural Resource Management, David L. Kulhavy, R Philip Reynolds, Daniel R. Unger, Matthew W. Mcbroom, I-Kuai Hung, Yanli Zhang

Faculty Publications

Digital preservation of library materials has increased the need for methods to access the documents and contents maintained in digital archives. The use of altmetrics to quantify the impact of scholarly works, including PlumX, is increasing readership by listing articles in reference services. The outreach from the digital repository ScholarWorks at Stephen F. Austin State University (SFASU) highlights the impact within the natural resources community from Digital Commons, Forest Sciences Commons; and from the Natural Products Chemistry and Pharmacognosy Commons. The use of PlumX altmetrics was examined to evaluate usage, impact, and digital audience downloads for the Arthur Temple College ...


Reconstruction Of Piñon–Juniper Woodlands In The Sky Islands Of The Davis Mountains, Texas, Usa, Mohammad Bataineh, Brian P. Oswald, Hans M. Williams, Dan Unger, I-Kuai Hung Oct 2020

Reconstruction Of Piñon–Juniper Woodlands In The Sky Islands Of The Davis Mountains, Texas, Usa, Mohammad Bataineh, Brian P. Oswald, Hans M. Williams, Dan Unger, I-Kuai Hung

Faculty Publications

Piñon (Pinus spp. L.)–juniper (Juniperus spp. L.) woodlands’ historical stand structures were recreated to provide reference conditions and document long‐term changes in the Sky Islands of the Davis Mountains, Texas. Restoration of these isolated woodlands requires insights into the range of variability in current and historical stand structures, as well as an understanding of the spatiotemporal establishment and recruitment patterns of tree species. With drastic changes in forests and woodlands of the Southwestern United States widely reported, the main objective of this study was to reconstruct woodland tree temporal and spatial establishment patterns. A stratified random sampling approach ...


The Dynamic Trophic Niche Of An Island Bird Of Prey, Ulises Balza, Nicolas A. Lois, Michael J. Polito, Klemens Puetz, Amira Saalom, Andrea Raya Rey Oct 2020

The Dynamic Trophic Niche Of An Island Bird Of Prey, Ulises Balza, Nicolas A. Lois, Michael J. Polito, Klemens Puetz, Amira Saalom, Andrea Raya Rey

Faculty Publications

Optimal foraging theory predicts an inverse relationship between the availability of preferred prey and niche width in animals. Moreover, when individuals within a population have identical prey preferences and preferred prey is scarce, a nested pattern of trophic niche is expected if opportunistic and selective individuals can be identified. Here, we examined intraspecific variation in the trophic niche of a resident population of striated caracara (Phalcoboenus australis) on Isla de los Estados (Staten Island), Argentina, using pellet and stable isotope analyses. While this raptor specializes on seabird prey, we assessed this population's potential to forage on terrestrial prey, especially ...


Fish Assemblages Associated With Artificial Reefs Assessed Using Multiple Gear Types In The Northwest Gulf Of Mexico, Jeffrey D. Plumlee, Kaylan M. Dance, Michael A. Dance, Hay R. Rooker, Thomas C. Tinhan, J. Brooke Shipley, R. J. David Wells Oct 2020

Fish Assemblages Associated With Artificial Reefs Assessed Using Multiple Gear Types In The Northwest Gulf Of Mexico, Jeffrey D. Plumlee, Kaylan M. Dance, Michael A. Dance, Hay R. Rooker, Thomas C. Tinhan, J. Brooke Shipley, R. J. David Wells

Faculty Publications

Quantitative surveys of fishes associated with artificial reefs in the northwest Gulf of Mexico were conducted over a 4-yr period (2014-2017). Artificial reefs surveyed were comprised of three types: concrete structures, rig jackets, and decommissioned ships. All reefs were surveyed using vertical long line ( VLL), fish traps, and Adaptive Resolution Imaging Sonar (ARIS 1800). Mean fish abundance did not significantly differ using VLL [1.7 ind set(-1) (SD 2.2)] among the three reef types. However, relative abundance among all fishes collected was significantly highest on rig reefs using traps [6.2 ind soak(-1) (SD 3.8)], while ...


Reducing Host Dna Contamination In 16s Rrna Gene Surveys Of Anthozoan Microbiomes Using Pna Clamps, Alicia M. Reigel, Sarah M. Owens, Michael E. Hellberg Oct 2020

Reducing Host Dna Contamination In 16s Rrna Gene Surveys Of Anthozoan Microbiomes Using Pna Clamps, Alicia M. Reigel, Sarah M. Owens, Michael E. Hellberg

Faculty Publications

Efforts to study the microbial communities associated with corals can be limited by inefficiencies in the sequencing process due to high levels of host amplification by universal bacterial 16S rRNA gene primers. Here, we develop an inexpensive peptide nucleic acid (PNA) clamp that binds to a target sequence of host DNA during PCR and blocks amplification. We then test the ability of this PNA clamp to mitigate host contamination and increase overall microbial sequence coverage on samples from three coral species: the gorgoniansEunicea flexuosaandGorgonia ventalina,and the scleractinianPorites panamensis. The 20-bp PNA clamp was designed using DNA fromE. flexuosa. Adding ...


All-Atomic Molecular Dynamic Studies Of Human And Drosophila Cdk8: Insights Into Their Kinase Domains, The Lxxll Motifs, And Drug Binding Site, Wu Xu, Xiao-Jun Xie, Ali K. Faust, Mengmeng Liu, Xiao Li, Feng Chen, Ashlin A. Naquin, Avery C. Walton, Peter W. Kishbaugh, Jun-Yuan Ji Oct 2020

All-Atomic Molecular Dynamic Studies Of Human And Drosophila Cdk8: Insights Into Their Kinase Domains, The Lxxll Motifs, And Drug Binding Site, Wu Xu, Xiao-Jun Xie, Ali K. Faust, Mengmeng Liu, Xiao Li, Feng Chen, Ashlin A. Naquin, Avery C. Walton, Peter W. Kishbaugh, Jun-Yuan Ji

Faculty Publications

Cyclin-dependent kinase 8 (CDK8) and its regulatory partner Cyclin C (CycC) play conserved roles in modulating RNA polymerase II (Pol II)-dependent gene expression. To understand the structure and function relations of CDK8, we analyzed the structures of human and Drosophila CDK8 proteins using molecular dynamics simulations, combined with functional analyses in Drosophila. Specifically, we evaluated the structural differences between hCDK8 and dCDK8 to predict the effects of the LXXLL motif mutation (AQKAA), the P154L mutations, and drug binding on local structures of the CDK8 proteins. First, we have observed that both the LXXLL motif and the kinase activity of ...


Oil-Producing Metabolons Containing Dgat1 Use Separate Substrate Pools From Those Containing Dgat2 Or Pdat, Anushobha Regmi, Jay Shockey, Hari Kiran Kotapati, Philip D. Bates Oct 2020

Oil-Producing Metabolons Containing Dgat1 Use Separate Substrate Pools From Those Containing Dgat2 Or Pdat, Anushobha Regmi, Jay Shockey, Hari Kiran Kotapati, Philip D. Bates

Faculty Publications

© 2020 American Society of Plant Biologists. All rights reserved. Seed triacylglycerol (TAG) biosynthesis involves a metabolic network containing multiple different diacylglycerol (DAG) and acyl donor substrate pools. This network of pathways overlaps with those for essential membrane lipid synthesis and utilizes multiple different classes of TAG biosynthetic enzymes. Acyl flux through this network ultimately dictates the final oil fatty acid composition. Most strategies to alter seed oil composition involve the overexpression of lipid biosynthetic enzymes, but how these enzymes are assembled into metabolons and which substrate pools are used by each is still not well understood. To understand the roles ...


No Mow May Lawns Have Higher Pollinator Richness And Abundances: An Engaged Community Provides Floral Resources For Pollinators, Relena R. Ribbons, Israel Del Toro Sep 2020

No Mow May Lawns Have Higher Pollinator Richness And Abundances: An Engaged Community Provides Floral Resources For Pollinators, Relena R. Ribbons, Israel Del Toro

Faculty Publications

No Mow May is a community science initiative popularized in recent years that encourages property owners to limit their lawn mowing practices during the month of May. The goal of No Mow May is to provide early season foraging resources for pollinators that emerge in the spring, especially in urban landscapes when few floral resources are available. We worked with the city council of Appleton, Wisconsin, USA. to allow No Mow May to take place in May 2020. Four hundred and thirty-five property owners registered for No Mow May in Appleton. We measured floral and bee richness and abundance in ...


Elementary Classroom Teachers’ Self-Reported Use Of Movement Integration Products And Perceived Facilitators And Barriers Related To Product Use, Roddrick Dugger, Aaron Rafferty, Ethan Hunt, Michael W. Beets, Collin Andrew Webster, Brian Chen, Jeffrey Michael Rehling, Robert Glenn Weaver Sep 2020

Elementary Classroom Teachers’ Self-Reported Use Of Movement Integration Products And Perceived Facilitators And Barriers Related To Product Use, Roddrick Dugger, Aaron Rafferty, Ethan Hunt, Michael W. Beets, Collin Andrew Webster, Brian Chen, Jeffrey Michael Rehling, Robert Glenn Weaver

Faculty Publications

Movement integration (MI) products are designed to provide children with physical activity during general education classroom time. The purpose of this study was to examine elementary classroom teachers’ self-reported use of MI products and subsequent perceptions of the facilitators of and barriers to MI product use. This study utilized a mixed-methods design. Elementary classroom teachers (n = 40) at four schools each tested four of six common MI products in their classroom for one week. Teachers completed a daily diary, documenting duration and frequency of product use. Following each product test, focus groups were conducted with teachers to assess facilitators and ...


Electronic-Cigarette Vehicles And Flavoring Affect Lung Function And Immune Responses In A Murine Model, Brittany N. Szafran, Rakeysha Pinkston, Zakia Perveen, Matthew K. Ross, Timothy Morgan, Daniel B. Paulsen, Arthur L. Penn, Barbara L. F. Kaplan, Alexandra Noel Sep 2020

Electronic-Cigarette Vehicles And Flavoring Affect Lung Function And Immune Responses In A Murine Model, Brittany N. Szafran, Rakeysha Pinkston, Zakia Perveen, Matthew K. Ross, Timothy Morgan, Daniel B. Paulsen, Arthur L. Penn, Barbara L. F. Kaplan, Alexandra Noel

Faculty Publications

The use of electronic nicotine delivery systems (ENDS), also known as electronic-cigarettes (e-cigs), has raised serious public health concerns, especially in light of the 2019 outbreak of e-cig or vaping product use-associated acute lung injury (EVALI). While these cases have mostly been linked to ENDS that contain vitamin E acetate, there is limited research that has focused on the chronic pulmonary effects of the delivery vehicles (i.e., without nicotine and flavoring). Thus, we investigated lung function and immune responses in a mouse model following exposure to the nearly ubiquitous e-cig delivery vehicles, vegetable glycerin (VG) and propylene glycol (PG ...


Behavioral Strategies During Incubation Influence Nest And Female Survival Of Wild Turkeys, Ashley K. Lohr, James A. Martin, Gregory T. Wann, Bradley S. Cohen, Bret A. Collier, Michael J. Chamberlain Sep 2020

Behavioral Strategies During Incubation Influence Nest And Female Survival Of Wild Turkeys, Ashley K. Lohr, James A. Martin, Gregory T. Wann, Bradley S. Cohen, Bret A. Collier, Michael J. Chamberlain

Faculty Publications

Females must balance physiological and behavioral demands of producing offspring with associated expenditures, such as resource acquisition and predator avoidance. Nest success is an important parameter underlying avian population dynamics. Galliforms are particularly susceptible to low nest success due to exposure of ground nests to multiple predator guilds, lengthy incubation periods, and substantive reliance on crypsis for survival. Hence, it is plausible that nesting individuals prioritize productivity and survival differently, resulting in a gradient of reproductive strategies. Fine-scale movement patterns during incubation are not well documented in ground-nesting birds, and the influence of reproductive movements on survival is largely unknown ...


Initial Establishment Success Of Five Forages In An East Texas Loblolly Pine (Pinus Taeda) Silvopasture, Michael Richard, Ken Farrish, Brian P. Oswald, Hans M. Williams, Michael Maurer Sep 2020

Initial Establishment Success Of Five Forages In An East Texas Loblolly Pine (Pinus Taeda) Silvopasture, Michael Richard, Ken Farrish, Brian P. Oswald, Hans M. Williams, Michael Maurer

Faculty Publications

The establishment at the end of 1 year of five forages was evaluated in a loblolly pine (Pinus taeda L.) silvopasture system. The five forages were: ‘Pensacola” bahiagrass (Paspalum notatum Fluegge), “Texas Tough” bermudagrass (Cynodon dactylon L. Pers.), “Alamo” switchgrass (Panicum virgatum L.), “San Marcos” Eastern gamagrass (Tripsacum dactyloides L.), and a native mix containing 45% “Texas” little bluestem (Schizachyrium scoparium Michx Nash), 15% sand lovegrass (Eragrostis trichodes Nutt. L. Alph. Wood), 15% “Blackwell” switchgrass (Panicum virgatum L.), 10% “Lometa” indiangrass (Sorgastrum nutans L. Nash), 10% “Haskell” sideoats grama (Bouteloua curtipendula Michx Torr) and 5% “Earl” big bluestem (Andropgon gerardii ...