Life Sciences Commons^™

Thrombin Rapidly Induces Protein Kinase D Phosphorylation, And Protein Kinase C Delta Mediates The Activation, M Tan, Xuemin Xu, M Ohba, W Ogawa, Mei-Zhen Cui

Mei-Zhen Cui

Thrombin plays a critical role in hemostasis, thrombosis, and inflammation. However, the responsible intracellular signaling pathways triggered by thrombin are still not well defined. We report here that thrombin rapidly and transiently induces activation of protein kinase D (PKD) in aortic smooth muscle cells. Our data demonstrate that protein kinase C (PKC) inhibitors completely block thrombin-induced PKD activation, suggesting that thrombin induces PKD activation via a PKC-dependent pathway. Furthermore, our results show that thrombin rapidly induces PKC delta phosphorylation and that the PKC delta-specific inhibitor rottlerin blocks thrombin-induced PKD activation, suggesting that PKC delta mediates the thrombin-induced PKD activation. Using ...

Lysophosphatidic Acid, Tissue Factor And Atherosclerosis (Invited Commentary), Mei-Zhen Cui, Xuemin Xu

Mei-Zhen Cui

http://www.athero.org

Lysophosphatidic Acid Induction Of Tissue Factor Expression In Aortic Smooth Muscle Cells, Mei-Zhen Cui, G Zhao, Al Winokur, E Laag, Jr Bydash, Ms Penn, Gm Chisolm, Xuemin Xu

Mei-Zhen Cui

OBJECTIVE: Tissue factor (TF), the initiator of the coagulation cascade, is expressed by cells in atherosclerotic lesions. Lysophosphatidic acid (LPA) is a component of oxidized lipoproteins and an agent released by activated platelets. The present study investigated whether and how TF expression is regulated by LPA. METHODS AND RESULTS: Northern blotting, Western blotting, and TF activity assays demonstrated that LPA markedly induced TF mRNA, protein, and activity in vascular smooth muscle cells. LPA-induced TF expression is primarily controlled at the transcriptional level. Phosphorylation of mitogen-activated protein kinase kinase (MEK) and extracellular signaling-regulated kinases (ERK1/2) was rapidly and markedly induced ...

Monitoring Of Levels And Tracking Of Listeria Monocytogenes Strains In A Seafood Processing Environment Using Enrichment Mpn And Rapd, Lynne Mclandsborough, C. Cronin, M. Clark, R. Witkowsky, H. Lui, A. Sayedahmed, R.E. Levin

M. Kathleen Clark

No abstract provided.

Monitoring Of Microflora Present Within A Seafood Processing Environment Using Plating Methodology And Denaturing Gradient Gel Electrophoresis, Lynne Mclandsborough, M. Avallone, M. Clark, R. Witkowsky, H. Lui, A. Sayedahmad, R.E. Levin

M. Kathleen Clark

No abstract provided.

Detection Of Feline Coronavirus Infection In Southern African Nondomestic Felids, Melissa Kennedy, Stephen Kania, E Stylianides, H Bertschinger, D Keet, M Van Vuuren

Stephen A Kania

Feline coronavirus (FCoV) infects members of the Felidae family with results ranging from seroconversion with no disease to fatal feline infectious peritonitis (FIP). Infection of non-domestic felids with FCoV is of concern, particularly in endangered populations such as cheetahs (Acinonyx jubatus). In this investigation, we tested 342 animals in the Republic of South Africa and Namibia, including 140 animals from wild populations, for evidence of FCoV infection by serology and/or reverse transcription/nested polymerase chain reaction (RT/nPCR) on feces from 1999 through 2001. Past or current infection was evaluated. Of these, 195 animals had evidence of infection and ...

Geobacter Sulfurreducens Can Grow With Oxygen As A Terminal Electron Acceptor, W. C. Lin, Maddalena V. Coppi, Derek Lovley

Derek Lovley

Geobacter sulfurreducens, previously classified as a strict anaerobe, tolerated exposure to atmospheric oxygen for at least 24 h and grew with oxygen as the sole electron acceptor at concentrations of 10% or less in the headspace. These results help explain how Geobacter species may survive in oxic subsurface environments, being poised to rapidly take advantage of the development of anoxic conditions.

A New Species Of Neoscelis From Jalisco, Mexico (Coleoptera: Scarabaeidae: Cetoniinae), Alan D. Mudge, Brett C. Ratcliffe, Richard L. Westcott, Felipe A. A. Noguera

Papers in Entomology

Neoscelis coracina Mudge et Ratcliffe sp. n., from Jalisco, Mexico, is described and illustrated. Intraspecific variation is discussed, and diagnostic characters separating N. coracina sp. n. from N. dohrni (Westwood, 1855) are given. The key to males of New World Goliathini is revised. Notes on habitat, behavior, and collecting N. coracina sp. n. are included. A new state record is provided for N. dohrni.

Natural Resources Outreach Coalition: Coordination And Program Delivery, Frank Mitchell

PREP Reports & Publications

The Natural Resources Outreach Coalition (NROC) is a multi-organizational initiative providing technical and educational assistance to communities in New Hampshire’s coastal watersheds dealing with the effects of growth. The NROC program offered to communities includes an initial educational public presentation, Dealing with Growth, followed by a series of follow-up meetings to help the community develop an action-oriented work plan, and provide the technical and educational assistance needed to help the community meet its goals.

Nonmuscle Myosin Iib Is Involved In The Guidance Of Fibroblast Migration, Chun-Min Lo, Denis B. Buxton, Gregory C. H. Chua, Micah Dembo, Robert S. Adelstein, Yu-Li Wang

Open Access Articles

Although myosin II is known to play an important role in cell migration, little is known about its specific functions. We have addressed the function of one of the isoforms of myosin II, myosin IIB, by analyzing the movement and mechanical characteristics of fibroblasts where this protein has been ablated by gene disruption. Myosin IIB null cells displayed multiple unstable and disorganized protrusions, although they were still able to generate a large fraction of traction forces when cultured on flexible polyacrylamide substrates. However, the traction forces were highly disorganized relative to the direction of cell migration. Analysis of cell migration ...

Collaborative Research: Origins Of Cods On Georges Bank: Contributions Of Early Developmental Stages For The Scotian Shelf, David W. Townsend, Irv Kornfield, Linda Kling

University of Maine Office of Research Administration: Grant Reports

Recent work in the Georges Bank-Gulf of Maine area has documented significant, and apparently episodic, fluxes of Scotian Shelf Water (SSW) from the Nova Scotian continental shelf to Georges Bank. SSW is a relatively cold and fresh water mass with a significant component from the St. Lawrence River, and is commonly identifiable with temperature-salinity analyses of hydrographic data and in satellite images of sea surface temperature. One such flux episode was observed last March (1997) in satellite imagery and from shipboard hydrographic sampling on Georges Bank. Qualitative at-sea analyses of ichthyoplankton sampled on the March cruise revealed a remarkably tight ...

Gbcw Support For Shellfish Activities 2003, B Sharon Meeker, Ann S. Reid

PREP Reports & Publications

The Great Bay Coast Watch (GBCW) is a volunteer estuarine monitoring program established in 1989 that includes teachers, students, and local citizens with a diversity of backgrounds. Volunteers participate in a variety of training programs that enable them to monitor water quality parameters in Great Bay and coastal areas, sample for marine phytoplankton blooms and conduct shoreline surveys and habitat evaluations. Since 1997 the New Hampshire Estuaries Project (NHEP) has relied on the ability of GBCW to recruit and train volunteers to assist with the implementation of its plan to protect, restore and manage the states estuarine systems. This year ...

Support For The 3rd Annual Exeter River Alewife Festival, Exeter River Local Advisory Committee

PREP Reports & Publications

With support from the NH Estuaries Project (NHEP), the Exeter River Local Advisory Committee (ERLAC) held the 3rd Annual Exeter River Alewife Festival on May 31, 2003 in downtown Exeter. NHEP funding enabled ERLAC and the Festival Planning Committee to print and distribute posters and cards advertising the Festival, purchase newspaper advertisements and arrange opportunities for the press to learn more about the event. In addition, ERLAC members worked with area newspapers to write and print eleven articles highlighting aspects of the river and the watershed.

The Zebrafish Iguana Locus Encodes Dzip1, A Novel Zinc Finger Protein Required For Proper Regulation Of Hedgehog Signaling, Kohshin Sekimizu, Noriyuki Nishioka, Hiroshi Sasaki, Hiroyuki Takeda, Rolf O. Karlstrom, Atsushi Kawakami

Rolf O Karlstrom

Members of the Hedgehog (Hh) family of intercellular signaling molecules play crucial roles in animal development. Aberrant regulation of Hh signaling in humans causes developmental defects, and leads to various genetic disorders and cancers. We have characterized a novel regulator of Hh signaling through the analysis of the zebrafish midline mutant iguana (igu). Mutations in igu lead to reduced expression of Hh target genes in the ventral neural tube, similar to the phenotype seen in zebrafish mutants known to affect Hh signaling. Contradictory at first sight, igu mutations lead to expanded Hh target gene expression in somites. Genetic and pharmacological ...

Soil Organic Carbon Content And Composition Of 130-Year Crop, Pasture And Forest Land-Use Managements, Dean A. Martens, Thomas E. Reedy, David T. Lewis (Retired)

Agronomy & Horticulture -- Faculty Publications

Conversion of former agricultural land to grassland and forest ecosystems is a suggested option for mitigation of increased atmospheric CO₂. A Sharpsburg prairie loess soil (fine, smectitic, mesic Typic Argiudoll) provided treatments to study the impact of long-term land use on soil organic carbon (SOC) content and composition for a 130-year-old cropped, pasture and forest comparison. The forest and pasture land use significantly retained more SOC, 46% and 25%, respectively, compared with cropped land use, and forest land use increased soil C content by 29% compared with the pasture. Organic C retained in the soils was a function of ...

Self-Aligned Mechanical Attachment Of Carbon Nanotubes To Silicon Dioxide Structures By Selective Silicon Dioxide Chemical-Vapor Deposition, Jed D. Whittaker, Ghaleb A. Husseini, Matthew A. Linford, Robert C. Davis, Markus Brink

All Faculty Publications

A self-aligned thin-film deposition technique was developed to mechanically attach carbon nanotubes to surfaces for the fabrication of structurally robust nanotube-based nanomechanical devices. Single-walled carbon nanotubes were grown by thermal chemical-vapor deposition (CVD) across 150-nm-wide SiO2 trenches. The nanotubes were mechanically attached to the trench tops by selective silicon tetraacetate-based SiO2 CVD. No film was deposited on the nanotubes where they were suspended across the trenches.

Disposition Of Installment Obligations, Neil E. Harl

Agricultural Law Digest

Other than for dispositions of installment obligations at death and certain tax-free exchanges, the privilege of income deferral by installment reporting is generally personal to the seller. Because of the potential for triggering income tax liability, all transactions involving installment obligations should be approached with care including transfer to revocable inter vivos trusts.

Index To Volume 14, Nos. 1-24, Agricultural Law Digest

Agricultural Law Digest

No abstract provided.

Cases, Regulations, And Statutes, Robert P. Achenbach Jr

Agricultural Law Digest

No abstract provided.

Swine Waste Demonstration And Training Project, C. V. Maxwell, K. Vandevender, K. P. Coffey, P. A. Moore, I. Chaubey, D. R. Smith

Technical Reports

No abstract provided.

The Dynastine Scarab Beetles Of Costa Rica And Panama (Coleoptera: Scarabaeidae: Dynastinae), Brett C. Ratcliffe

Bulletin of the University of Nebraska State Museum

The 157 species of dynastine scarab beetles that occur in Costa Rica and Panama are comprehensively reviewed. Keys, descriptions, distributions, and notes on biology are provided for all species as well as illustrations and maps. The larvae of Enema endymion, Heterogomphus chevrolati, and Dynastes hercules are described for the first time. Also included are synopses of the higher-level taxa, a glossary, a gazetteer of place names, and a species checklist.

The following new species are described: Cyclocephala alazona, C. enigma, C. labidion, C. marylizae, C. mustacha, C. stockwelli, C. una mas (all Cyclocephalini); Irazua dilicra (Oryctini); Amblyoproctus centroamericanus, Hemiphileurus curoei ...

An Accurate Vo2max Non-Exercise Regression Model For 18 To 65 Year Old Adults, Danielle I. Bradshaw

All Theses and Dissertations

The purpose of this study was to develop a regression equation to predict VO2max based on non-exercise (N-EX) data. All participants (N = 100), aged 18-65 years old, successfully completed a maximal graded exercise test (GXT) to assess VO2max (mean ± SD; 39.96 mL∙kg-¹∙min&sup-1; ± 9.54 mL∙kg-¹∙min-¹). The N-EX data collected just before the maximal GXT included the participant's age, gender, body mass index (BMI), perceived functional ability (PFA) to walk, jog, or run given distances, and current physical activity (PA-R) level. Multiple linear regression generated the following N-EX prediction equation (R = .93, SEE = 3.45 ...

Seasonal Variation And Ecosystem Dependence Of Emission Factors For Selected Trace Gases And Pm2.5 For Southern African Savanna Fires, S. Korontzi, Darold E. Ward, Ronald A. Susott, Robert J. Yokelson, C. O. Justice, Peter V. Hobbs, E.A. H. Smithwick, Wei Min Hao

Chemistry and Biochemistry Faculty Publications

[1] In this paper we present the first early dry season (early June-early August) emission factor measurements for carbon dioxide (CO₂), carbon monoxide (CO), methane (CH₄), nonmethane hydrocarbons (NMHC), and particulates with a diameter less than 2.5 μm (PM_2.5) for southern African grassland and woodland fires. Seasonal emission factors for grassland fires correlate linearly with the proportion of green grass, used as a surrogate for the fuel moisture content, and are higher for products of incomplete combustion in the early part of the dry season compared with later in the dry season. Models of emission ...

Northeast Research Station Watertown, South Dakota Annual Progress Report, 2003, Agricultural Experiment Station, Plant Science Department

Agricultural Experiment Station and Research Farm Annual Reports

This is the 2003 annual progress report for the Northeast Research Station in Watertown, South Dakota. This report is issued by the South Dakota Agricultural Experiment Station and the South Dakota State University Plant Science Department. This report includes weather data, yield comparisons, crop performance trials, corn trials, soybean trials, winter wheat performance testing, Barley Foliar information, weed and pest control, herbicide demonstrations, canola and flax variety trials and more.

Assembly Of Α4Β2 Nicotinic Acetylcholine Receptors Assessed With Functional Fluorescently Labeled Subunits: Effects Of Localization, Trafficking, And Nicotine-Induced Upregulation In Clonal Mammalian Cells And In Cultured Midbrain Neurons, Raad Nashmi, Mary E. Dickinson, Sheri Mckinney, Mark Jareb, Cesar Labarca, Scott E. Fraser E. Fraser, Henry A. Lester

Biology Faculty Publications

Fura-2 recording of Ca²⁺ influx was used to show that incubation in 1 μM nicotine (2–6d) upregulates several pharmacological components of acetylcholine (ACh) responses in ventral midbrain cultures, including a MLA-resistant, DHβE-sensitive component that presumably corresponds to α4β2 receptors. To study changes in α4β2 receptor levels and assembly during this upregulation, we incorporated yellow and cyan fluorescent proteins (YFPs and CFPs) into the α4 or β2 M3–M4 intracellular loops, and these subunits were coexpressed in human embryonic kidney (HEK) 293T cells and cultured ventral midbrain neurons ...

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The National Pork Producers Council Maternal Line National Genetic Evaluation Program: A Comparison Of Six Maternal Genetic Lines For Female Productivity Measures Over Four Parities, J. S. Moeller, R. K. Johnson, J. W. Mabry, T. J. Baas, O. W. Robison

Faculty Papers and Publications in Animal Science

Litter (n = 8,424) and female performance records were collected in two breed-to-wean production units in order to evaluate genetic line differences for sow longevity and maternal performance over four parities. Lines evaluated were American Diamond Genetics, Danbred North America, Dekalb-Monsanto DK44, Dekalb-Monsanto GPK347, Newsham Hybrids, and National Swine Registry. Females within a line were derived from a minimum of 65 sires, 197 dams (three dams per sire), and a maximum of three daughters per dam, except in the GPK347, which were produced using semen from 12 Nebraska Index boars mated with Dekalb-Monsanto Line 34 females. All lines expressed 100 ...

Evaluation Of Gene Expression In Pigs Selected For Enhanced Reproduction Using Differential Display Pcr: Ii. Anterior Pituitary, G. R. Bertani, C. D. Gladney, R. K. Johnson, Daniel Pomp

Faculty Papers and Publications in Animal Science

The objective of this study was to identify differentially expressed genes in the anterior pituitary (AP) of sows selected for enhanced reproductive phenotypes. Selection in the Index (I) line was based on an index of ovulation rate and embryo survival, whereas random selection was used in the Control (C) line. Average numbers of fully formed piglets at birth were 12.5 ± 1.5 and 9.9 ± 2.0 for Line I and C sows used in this study, respectively. In order to induce luteolysis and synchronize follicle development, sows were injected (i.m.) with 2 mL of prostaglandin F_{2α ...}

Lambda Red-Mediated Recombinogenic Engineering Of Enterohemorrhagic And Enteropathogenic E. Coli, Kenan C. Murphy, Kenneth Geno Campellone

Open Access Articles

BACKGROUND: The lambda Red recombineering technology has been used extensively in Escherichia coli and Salmonella typhimurium for easy PCR-mediated generation of deletion mutants, but less so in pathogenic species of E. coli such as EHEC and EPEC. Our early experiments with the use of lambda Red in EHEC and EPEC have led to sporadic results, leading to the present study to identify factors that might improve the efficiency of Red recombineering in these pathogenic strains of E. coli. RESULTS: In this report, we have identified conditions that optimize the use of lambda Red for recombineering in EHEC and EPEC. Using ...

Crop Rotation Considerations For 2004 Management Season Rotation, Mahdi Al-Kaisi, H. Mark Hanna, Michael Tidman

Integrated Crop Management News

There are many management strategies for improving soil productivity. Crop rotation or cropping sequence is proven to be very effective in addressing concerns related to soil, water, and environment quality from long- or short-term perspectives. Producers who are innovative in diversifying their cropping systems and management strategies will be more successful than others who are not.