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Full-Text Articles in Life Sciences

Project Alien, Rebecca Mccallin, Madelyn Hoying, Alex Evans, Matthew Nestler, Karli Rae Sutton, Garett Craig, Lucia Secaia Del Cid, Alexander Guy, Rachel Fernandez, Amanda Trusiak, Paige Aley, Ingabire Gakwerere, Nina Dorfner, Maria Mosbacher, Mary Flavin, Selvin Hernandez, Audrey Steen, Benjamin Kazimer May 2021

Project Alien, Rebecca Mccallin, Madelyn Hoying, Alex Evans, Matthew Nestler, Karli Rae Sutton, Garett Craig, Lucia Secaia Del Cid, Alexander Guy, Rachel Fernandez, Amanda Trusiak, Paige Aley, Ingabire Gakwerere, Nina Dorfner, Maria Mosbacher, Mary Flavin, Selvin Hernandez, Audrey Steen, Benjamin Kazimer

Undergraduate Research and Scholarship Symposium

Project ALIEN is a comprehensive plan to send humans to Mars to look for life on the Martian surface while exploring the viability and adaptability of terrestrial microbes in Martian atmospheric conditions. ALIEN will use a ballistic capture trajectory to get to Mars and stay in aerostationary orbit for a 30-day surface mission, during which two surface crewmembers will perform a variety of experiments to achieve the mission’s goals of Martian microbial discovery within brines of the Gale Crater and terrestrial microbe adaptability and viability to Martian conditions. Experimentation is based on the presumption that Martian microbes are metabolically ...


Profile Of Antimicrobial Susceptibility From Cattles’S Milk Isolates Suffering From Mastitis In District Lahore, Muhammad Imran, Iqra Rehman, Abdul Qayyum Khan Sulehria, Yasser Mustafa Butt, Abdul Majid Khan, Azizah Ziauddin May 2021

Profile Of Antimicrobial Susceptibility From Cattles’S Milk Isolates Suffering From Mastitis In District Lahore, Muhammad Imran, Iqra Rehman, Abdul Qayyum Khan Sulehria, Yasser Mustafa Butt, Abdul Majid Khan, Azizah Ziauddin

Journal of Bioresource Management

Mastitis is highly prevalent infection in cattle causing cost-effective loss in dairy milk production. Escherichia coli is the most frequently isolated bacteria causing mastitis worldwide. The current study was performed to investigate the mastitis prevalence and effect of different antibiotics against pathogens causing it. In sum, 216 milk samples were collected randomly including 108 each from both cows and buffaloes. These samples were subjected to Surf Field Mastitis Test for prevalence of mastitis that found 18.50% and 23.14% in buffaloes and cows respectively. Mastitis was more prevalent both in buffaloes and cows at the age of 9-10 years ...


Purification Of Pcbp2 And Its Effect On The Formation Of Dna I-Motifs And Secondary Structures, Nathan Redden May 2021

Purification Of Pcbp2 And Its Effect On The Formation Of Dna I-Motifs And Secondary Structures, Nathan Redden

Honors Theses

Several secondary, non-B forms of DNA are associated with the promoter regions of many oncogenes, including the intensely studied c-MYC gene that codes for a transcription factor. Two such secondary structures, G-quadruplexes, and i-motifs, have become topics of interest due to their prevalence within oncogenic promoters, as well as their potential accessibility for drug targeting. B-form DNA can adopt alternative structures under certain conditions. These alternative structures are favored when there is an asymmetric distribution of guanosine and cytosine on complimentary DNA strands. The strand rich in guanosine is able to form a quadruple stranded DNA complex termed a G-quadruplex ...


Partial Expression Of The Vbss Gene In Rhizobium Leguminosarum Atcc 14479 And In-Silico Analysis Of The Vbs Gene Cluster In Various Microorganisms, Afreen Siddiqui May 2021

Partial Expression Of The Vbss Gene In Rhizobium Leguminosarum Atcc 14479 And In-Silico Analysis Of The Vbs Gene Cluster In Various Microorganisms, Afreen Siddiqui

Undergraduate Honors Theses

Iron is extremely important for many organisms. Despite its abundancy, it exists in insoluble forms that makes its usability difficult. Some organisms secrete siderophores, low molecular weight compounds, that can chelate iron and convert it into usable forms for cells. One such organism, Rhizobium leguminosarum, is a nitrogen fixing symbiont proteobacteria that infects leguminous plants. The genome of Rhizobium leguminosarum ATCC 14479, which infects the red clover, Trifoli pratense, has previously been completely sequenced in our lab. Our lab has identified several genes in this strain involved in the biosynthesis of a siderophore, vicibactin. The protein product of one of ...


How Acetylation Regulates Metabolic Enzyme Function During Environmental Shifts, Jared Canonigo May 2021

How Acetylation Regulates Metabolic Enzyme Function During Environmental Shifts, Jared Canonigo

Biological Sciences Undergraduate Honors Theses

Organisms such as Saccharomyces cerevisiae can regulate the mechanisms of proteins through post-translational modification. These modifications play a vital role in functional proteomic activity because they can regulate protein activity, localization, and interaction with other cellular molecules. Such modifications include phosphorylation, methylation, and acetylation. The metabolic mechanisms of yeast became of keen interest to our lab because our lab noticed many stress defense proteins were being acetylated during stress heat shock. Notably, Adh1p and Adh2p showed both an increase and a decrease in acetylation at two lysine residues (K315 and K314) overtime during heat shock respectively, though the exact function ...


Painting With Pathogens, Emily List May 2021

Painting With Pathogens, Emily List

Chancellor’s Honors Program Projects

No abstract provided.


A Metabolomics-Based Approach To The Screening Of Endometrial Cancer: Development Of A Gas Chromatography-Ion Trap Mass Spectrometry-Based Method, Allycia Lee May 2021

A Metabolomics-Based Approach To The Screening Of Endometrial Cancer: Development Of A Gas Chromatography-Ion Trap Mass Spectrometry-Based Method, Allycia Lee

Honors Theses

Endometrial Cancer (EC) is the most common malignant tumor of the female reproductive tract. Currently there are no methods for effectively screening EC, but one method that is thought to be useful in screening EC is metabolomics. Metabolomics involves the analysis of metabolites which are low molecular weight inorganic and organic chemicals that are substrates, intermediates, and by-products of enzyme-mediated biochemical reactions in the cell. Previous research shows that the metabolic signature of EC patients are discernable from those of healthy patients. To aid in the development of an effective screening method for EC, blood serum, urine, and saliva samples ...


Increasing Stress Tolerance In Saccharomyces Cerevisiae Using Lipocalin Genes, Ximena Leon May 2021

Increasing Stress Tolerance In Saccharomyces Cerevisiae Using Lipocalin Genes, Ximena Leon

Honors Theses

Saccharomyces cerevisiae is a microorganism that is commonly used in industries, but its productivity is significantly hindered by its inability to resist high stress levels found in industrial processes. By increasing stress tolerance in S. cerevisiae, its role in industrial processes could be maximized. The objective of this study is to determine if complementation of lipocalin genes into the wildtype and knockout strains of S. cerevisiae can increase stress tolerance against a variety of stressors. We identified the knockout yeast strains, ALD3, ALD4, PDX3, and ILV1, as being sensitive to various stressors including salinity, heat, oxidative, and osmotic stresses. Arabidopsis ...


Antibiotic Resistance Crisis Spurring Phage Therapy Research, Cameron Miguel Perry May 2021

Antibiotic Resistance Crisis Spurring Phage Therapy Research, Cameron Miguel Perry

Chancellor’s Honors Program Projects

No abstract provided.


Antibiotic Resistance, A Global Pandemic, Deneil Patel May 2021

Antibiotic Resistance, A Global Pandemic, Deneil Patel

Chancellor’s Honors Program Projects

No abstract provided.


Resistance Of The Freshwater Microbiome To A Combination Of Pesticides, Gabriel Hooper May 2021

Resistance Of The Freshwater Microbiome To A Combination Of Pesticides, Gabriel Hooper

Senior Honors Theses

Pesticides are used prevalently throughout the United States and can negatively affect non-target organisms. Rain run-off can move multiple pesticides into freshwater ecosystems and bring them in contact with non-target organisms that have variable tolerances to pollution. This study seeks to improve our understanding of how an acute exposure to a combination of pesticides may restructure microbial communities by examining the microbiome before and after a 48-hour exposure to 2000 ppb of herbicides (glyphosate, atrazine, and 2,4-D). Pesticide exposure did not significantly alter the alpha or beta diversity of the sample types, which demonstrates the ability of freshwater microbiomes ...


Finding The Balance​ The Effects Of Α-Cyclodextrin, 2-Hydroxypropyl-Β-Cyclodextrin, And Cholesterol Bacteroides Vulgatus And Clostridium Bolteae​, Bethany Weaver May 2021

Finding The Balance​ The Effects Of Α-Cyclodextrin, 2-Hydroxypropyl-Β-Cyclodextrin, And Cholesterol Bacteroides Vulgatus And Clostridium Bolteae​, Bethany Weaver

Honors Program Projects

Atherosclerosis is a cardiovascular disease that is characterized by the hardening of arteries through the formation of cholesterol plaques. Cyclodextrins could potentially treat atherosclerosis by shrinking plaques. These cyclic oligosaccharides can make complexes with cholesterol but have also shown toxic side effects. This study looked for potential negative effects of cyclodextrins and cholesterol on gut bacteria. It was hypothesized that Bacteroides vulgatus will have decreased growth when grown in broth with cholesterol. In contrast, Clostridium bolteae will have decreased growth when grown in broth with cyclodextrins. Due to the fact that these bacteria are anaerobic, Clostridium bolteae and Bacteroides vulgatus ...


Proteomic Analysis Of Mycobacteriophage Crimd, William Moeller May 2021

Proteomic Analysis Of Mycobacteriophage Crimd, William Moeller

Undergraduate Honors Theses

Bacteriophages represent a large portion of the biomatter on our planet, and many of them have yet to be fully characterized. Here we discuss the proteomic analysis of a particular Bacteriophage, Mycobacteriophage CrimD. This phage was discovered on the Campus of William & Mary and has had its genome characterized. We took the next logical step of proteomic analysis.

In our analyses we made use high pressure liquid chromatography paired with linear ion trap mass spectrometry to analyze the proteome of CrimD at specific time points after the infection of its host, Mycobacterium smegmatis. Additionally, we used nanospray ionization with in-house ...


Effects On The Human Microbiome: Antibiotics And Other Chemicals, Katherine Moulton Apr 2021

Effects On The Human Microbiome: Antibiotics And Other Chemicals, Katherine Moulton

Thinking Matters Symposium

With skin being the largest organ of the human body, there is a very large surface area for a microbiome to form and live. The human microbiome is composed of different types of bacteria, fungi, and archaea, in different locations of the body. This layer can be greatly affected by daily practices including, hygiene habits, exercise, and antibiotic uses. The major objective of this study is to identify species of the human microbiome from various body sites and test their resistance to major chemicals. To achieve this objective, the mouth, ear, axillary area, hand, belly button, and foot of several ...


Bioremediation: Breaking Down The Regulations Of Genetically Modified Microorganisms, Lora Katharine Naismith Apr 2021

Bioremediation: Breaking Down The Regulations Of Genetically Modified Microorganisms, Lora Katharine Naismith

Texas A&M Law Review

Environmental bioremediation is the use of biological activity to reduce the concentration or toxicity of a pollutant. A rapidly increasing population leads to a consequential increase in industrial waste and pollution, and innovators are researching numerous techniques to degrade these pollutants and prevent their spread into the environment. These techniques are expensive and often result in secondary pollutants, which limits their widespread application. Bioremediation, however, presents a cost-friendly and more efficient way to degrade pollutants with little or no secondary pollutants. This Article explores how scientists can use genetically modified microorganisms (“GMMs”) to target specific hazardous wastes that are otherwise ...


Isolation Of Novel Thermophilic Actinomycetes From Hot Soils Of Centralia, Pennsylvania, Pamela Perez Apr 2021

Isolation Of Novel Thermophilic Actinomycetes From Hot Soils Of Centralia, Pennsylvania, Pamela Perez

Senior Scholars Day

No abstract provided.


Process Interventions For Improving The Microbiological Safety Of Low Moisture Food Ingredients, Tushar Verma Apr 2021

Process Interventions For Improving The Microbiological Safety Of Low Moisture Food Ingredients, Tushar Verma

Dissertations, Theses, & Student Research in Food Science and Technology

The recurrence of Salmonella in low moisture foods and the implementation of the FSMA rule requires a need to validate legacy and novel processing technologies. In this dissertation, a legacy thermal (extrusion), a novel thermal (radiofrequency (RF) heating), and a non-thermal (chlorine dioxide) technology, were evaluated as intervention technologies for Salmonella in low moisture foods. The twin-screw extruder was performed at different levels of screw speeds, temperatures, moisture contents, and fat contents to understand the impact of processing conditions on Salmonella inactivation in oat flour. At temperature >65°C, the Salmonella population was below the detection limit. At 55°C ...


Hiv-1 Drug Resistance To Integrase Strand Transfer Inhibitors In Hiv-1 Non-B Subtypes, Emmanuel Ndashimye Apr 2021

Hiv-1 Drug Resistance To Integrase Strand Transfer Inhibitors In Hiv-1 Non-B Subtypes, Emmanuel Ndashimye

Electronic Thesis and Dissertation Repository

Human immunodeficiency syndrome (HIV-1) has infected over 75 million people and over 35 million have succumbed to virus related illnesses. Despite access to a variety of antiretroviral therapy (ART) options, ART programs have been disproportionally spread in the world with low-and middle-income countries (LMICs) facing challenges to access the most potent ART options. With less potent ART remaining in use in LMICs, HIV-1 drug resistance (HIVDR) presents a growing challenge in LMICs. Since approval of the first-generation integrase strand transfer inhibitor (INSTIs), Raltegravir (RAL) in 2007, INSTIs remain the best choice as a backbone of ART. Access to second generation ...


Microbially-Mediated Carbonate Dissolution And Precipitation; Towards A Protocol For Ex-Situ, Cave-Analogue Cultivation Experiments, Vanessa E. Johnston, Andrea Martín-Pérez, Sara Skok, Janez Mulec Apr 2021

Microbially-Mediated Carbonate Dissolution And Precipitation; Towards A Protocol For Ex-Situ, Cave-Analogue Cultivation Experiments, Vanessa E. Johnston, Andrea Martín-Pérez, Sara Skok, Janez Mulec

International Journal of Speleology

Subterranean calcite dissolution and precipitation are often considered as strictly geochemical processes. The active involvement of microbes in these processes is commonly underestimated in the literature due to general oligotrophic conditions in caves, except in particular cave conditions, such as sulfidic caves and moonmilk deposits, where the presence of microbes likely plays a key role in mineral deposition. Here, we study the possible involvement of microbes from Postojna Cave, Slovenia, in carbonate dissolution (litholysis) and precipitation (lithogenesis). Microbes were sampled from small pools below hydrologically diverse drip sites and incubated on polished limestone tablets at 10 and 20°C for ...


Microplastics: Nacogdoches County’S Hidden Threat, Jordan Stanford, Sydney Matlock, Courtney Sims, Samantha Montes Apr 2021

Microplastics: Nacogdoches County’S Hidden Threat, Jordan Stanford, Sydney Matlock, Courtney Sims, Samantha Montes

Undergraduate Research Conference

Microplastics and their threat to marine environments are well known, but studies in freshwater environments are few and far between. Microplastics can be created by the breaking down of plastics or in the process of making plastics. They affect biological lifeforms in a multitude of ways, water temperature, permeability of the sediment. Pollution in Texas waterways have motivated groups like the Environmental Protection Agency, Trash Free Texas, and Environment Texas to get involved. The objectives of this study are two analyze Bonita and La Nana Bayou for microplastic presence and to educate and create awareness for Nacogdoches County on microplastics ...


B25: Generation And Characterization Of Sars-Cov-2 Spike Protein-Pseudotyped Lentivirus Particles For Use In Neutralizing Antibody Titer Testing Of Human Serum, Luis Sanchez Apr 2021

B25: Generation And Characterization Of Sars-Cov-2 Spike Protein-Pseudotyped Lentivirus Particles For Use In Neutralizing Antibody Titer Testing Of Human Serum, Luis Sanchez

Annual Research Symposium

No abstract provided.


B16: Chikungunya Virus Time Course Infection Of Human Macrophages, Madison Gray, Israel Guerrero-Arguero, Antonio Solis Leal, Richard Robison, Brad Burges, Brett Pickett Apr 2021

B16: Chikungunya Virus Time Course Infection Of Human Macrophages, Madison Gray, Israel Guerrero-Arguero, Antonio Solis Leal, Richard Robison, Brad Burges, Brett Pickett

Annual Research Symposium

Chikungunya virus (CHIKV) is an Alphavirus spread by Aedes spp. mosquitoes and is responsible for infecting 1.1 million people per year worldwide, including a large epidemic in the western hemisphere in 2014-2015. During the body’s immune response to CHIKV, human macrophages become infected after phagocytosis of CHIKV and undergo induced apoptosis, catalyzing the virus spread in the body. It is presently unclear what macrophage genes, functions, and intracellular signaling pathways are impacted during the early, intermediate, and late stages of CHIKV infection. Therefore we quantified the transcriptional response of human macrophage cells infected with CHIKV at two different ...


B12: The T Cell Co-Receptor Cd5 Alters Mouse Behavior And Gut Microbiome Composition, Kyle Reaveley Apr 2021

B12: The T Cell Co-Receptor Cd5 Alters Mouse Behavior And Gut Microbiome Composition, Kyle Reaveley

Annual Research Symposium

No abstract provided.


Seasonal Variation Of E. Coli Abundance And Antibiotic Resistance Patterns In Water And Sediment Samples From Two Creeks With Different Potential Fecal Contamination Sources., James S. Zumhagen, Timothy Gsell Apr 2021

Seasonal Variation Of E. Coli Abundance And Antibiotic Resistance Patterns In Water And Sediment Samples From Two Creeks With Different Potential Fecal Contamination Sources., James S. Zumhagen, Timothy Gsell

GSU Research Day

Escherichia coli has served as the primary indicator for fecal contamination for the past 30 years, but more recent studies suggest that this bacteria may survive the cold weather in the soil making it difficult to tell whether it is new contamination or old contamination. Studies have found that exposure to various contaminants such as heavy metals, water treatment chemicals, agricultural runoff, fecal contamination, and antibiotics, creates an increase in the resistance of the microbes exposed to it. Wastewater treatment facilities, animal hospitals and wastewater sites increase the bacterial load and the prevalence of antibiotic resistance of bacteria residing bacteria ...


Characterization Of Novel Chlorovirus Glycosyltransferases That Synthesize Atypical Glycans, Eric Noel Apr 2021

Characterization Of Novel Chlorovirus Glycosyltransferases That Synthesize Atypical Glycans, Eric Noel

Dissertations and Theses in Biological Sciences

Giant dsDNA chloroviruses encode a diverse repertoire of glycosyltransferases (GTases) and methyltransferases (MTases) that biosynthesize unusual, methylated sugars independent of their host chlorella-like green algae prompting a reexamination of glycobiology systems. Unlike most other viruses, the prototype chlorovirus PBCV-1 encodes most, if not all, of the machinery required to glycosylate its major capsid protein (MCP). The structures of the four N-linked glycans do not resemble any other glycans in the three domains of life. Here, we investigated the potential involvement of chlorovirus- encoded putative GTases and MTases in glycosylation of the viral MCP. First, we aimed to generate site-directed ...


Virtualized Clinical Studies To Assess The Natural History And Impact Of Gut Microbiome Modulation In Non-Hospitalized Patients With Mild To Moderate Covid-19 A Randomized, Open-Label, Prospective Study With A Parallel Group Study Evaluating The Physiologic Effects Of Kb109 On Gut Microbiota Structure And Function: A Structured Summary Of A Study Protocol For A Randomized Controlled Study, John P. Haran, Jose C. Pinero, Yan Zheng, Norma Alonzo Palma, Mark Wingertzahn Apr 2021

Virtualized Clinical Studies To Assess The Natural History And Impact Of Gut Microbiome Modulation In Non-Hospitalized Patients With Mild To Moderate Covid-19 A Randomized, Open-Label, Prospective Study With A Parallel Group Study Evaluating The Physiologic Effects Of Kb109 On Gut Microbiota Structure And Function: A Structured Summary Of A Study Protocol For A Randomized Controlled Study, John P. Haran, Jose C. Pinero, Yan Zheng, Norma Alonzo Palma, Mark Wingertzahn

University of Massachusetts Medical School Faculty Publications

OBJECTIVES: These 2 parallel studies (K031 and K032) aim to evaluate the safety of KB109 in addition to supportive self-care (SSC) compared with SSC alone in outpatients with mild to moderate coronavirus disease 2019 (COVID-19). KB109 is a novel synthetic glycan that was formulated to modulate the gut microbiome composition and metabolic output in order to increase beneficial short-chain fatty acid (SCFA) production in the gut. The K031 study is designed to evaluate the safety of KB109 and characterize its impact on the natural progression of COVID-19 in patients with mild to moderate disease. The K032 study is evaluating the ...


College Of Natural Sciences Newsletter, April 2021, College Of Natural Sciences Apr 2021

College Of Natural Sciences Newsletter, April 2021, College Of Natural Sciences

College of Natural Sciences Newsletters and Reports

Volume 2, Issue 4

Page 1 Dean's Message
Page 2 & 3 Awards & Recognition
Page 4 Student Spotlight: Allsion Bjornstad
Page 5 Research Spotlight: Dr. Vondruska & Students
Page 6 Department Head Interviews
Page 7 Media Coverage of CNS
Page 8 Open PRAIRIE Data
Page 9 Student Club Spotlight: BMGSA
Page 10 Then & Now: Urban Geography


Effectiveness Of Pathogen-Specific Passive Antibodies To Mitigate Infectious Diseases In Apis Mellifera, Tanner Nordseth Apr 2021

Effectiveness Of Pathogen-Specific Passive Antibodies To Mitigate Infectious Diseases In Apis Mellifera, Tanner Nordseth

Honors Thesis

Honeybees (Apis mellifera) are widely recognized as a vital part of the global ecosystem and the world's food supply due to their pivotal role in the pollination of both natural and agricultural flora. Colony Collapse Disorder (CCD) is an emerging phenomenon characterized by a colony's worker bees deserting the hive and leaving the queen behind. This usually results in colony failure. CCD is a multifactorial issue, with many environmental stressors and pathogens playing a role. Deformed Wing Virus (DWV) has been identified as a leading cause in this phenomenon. Paenibacillus larvae (P. larvae) is another lethal pathogen that ...


A Literature Review On Tumor Microenvironmental Immunosuppressive Mechanisms On Cd8+ T Lymphocytes That Contribute To Tumor Immune Evasion, Jessica R. Gabrysiak Apr 2021

A Literature Review On Tumor Microenvironmental Immunosuppressive Mechanisms On Cd8+ T Lymphocytes That Contribute To Tumor Immune Evasion, Jessica R. Gabrysiak

Honors Projects

The concept of tumor immune evasion is a prevalent obstacle in cancer research and the development of immunotherapies. Increased insight into mechanisms that are responsible for the escape of cancer cells from the immune system will help to improve therapeutic approaches and provide understanding into slowing disease progression by utilizing the body's immune system. With immunotherapy development on the forefront of cancer research due to seemingly attenuated toxicity and resistance when compared to traditional chemoradiotherapy, analyzing these obstacles is essential for emerging roles and future directions in the discipline of immuno-oncology. Understanding the immunosuppressive molecules that contribute to the ...


Niche Differentiation Of Bacteria And Fungi In Carbon And Nitrogen Cycling Of Different Habitats In A Temperate Coniferous Forest: A Metaproteomic Approach, Robert Starke, Rubén López Mondéjar, Zander Rainer Human, Diana Navrátilová, Martina Štursová, Tomáš Větrovský, Heather M. Olson, Daniel J. Orton, Stephen J. Callister, Mary S. Lipton, Adina Howe, Lee Ann Mccue, Christa Pennacchio, Igor Grigoriev, Petr Baldrian Apr 2021

Niche Differentiation Of Bacteria And Fungi In Carbon And Nitrogen Cycling Of Different Habitats In A Temperate Coniferous Forest: A Metaproteomic Approach, Robert Starke, Rubén López Mondéjar, Zander Rainer Human, Diana Navrátilová, Martina Štursová, Tomáš Větrovský, Heather M. Olson, Daniel J. Orton, Stephen J. Callister, Mary S. Lipton, Adina Howe, Lee Ann Mccue, Christa Pennacchio, Igor Grigoriev, Petr Baldrian

Agricultural and Biosystems Engineering Publications

Temperate coniferous forests sustain the highest levels of biomass of all terrestrial ecosystems and belong to the major carbon sinks on Earth. However, the community composition and its functional diversity depending on the habitat have yet to be unveiled. Here, we analyzed the proteomes from litter, plant roots, rhizosphere, and bulk soil in a temperate coniferous forest at two time points to improve the understanding of the interplay between bacterial and eukaryotic communities in different habitats. Our metaproteomic approach yielded a total of 139,127 proteins that allowed to differentiate the contribution of microbial taxa to protein expression as well ...