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Theses and Dissertations

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Full-Text Articles in Life Sciences

Effects Of Heme Oxygenase 1 Inducer, T-Bhq On Growth Of Multiple Myeloma Cell Lines, And On Osteoblast And Osteoclast Differention, Alyaa Alansari May 2021

Effects Of Heme Oxygenase 1 Inducer, T-Bhq On Growth Of Multiple Myeloma Cell Lines, And On Osteoblast And Osteoclast Differention, Alyaa Alansari

Theses and Dissertations

Expansion of plasma cells within the bone marrow constitutes the onset of multiple myeloma (MM). This disease manifests clinically primarily through the formation of osteolytic bone lesions that can lead to osteoporosis. The reason for the development of such lesions is the disruption of the equilibrium between bone resorption and bone formation as a result of proliferation of osteoclasts and reduction in the number of osteoblasts in the process of differentiation of mesenchymal stem cells (MSCs). The maintenance of bone architecture is critically dependent on osteoblasts and osteoclasts, the activity of which is underpinned by a range of soluble factors ...


Evolution And Selection: From Suppression Of Metabolic Deficiencies To Bacteriophage Host Range And Resistance, Daniel Kurt Arens Apr 2021

Evolution And Selection: From Suppression Of Metabolic Deficiencies To Bacteriophage Host Range And Resistance, Daniel Kurt Arens

Theses and Dissertations

The evolution and adaptation of microorganisms is so rapid it can be seen in the time frame of days. The root cause for their evolution comes from selective environmental pressures that see organisms with beneficial mutations survive otherwise deadly encounters or outperform members of its population who fail to adapt. This does not always result in strict improvement of the individual as in the case of antibiotic resistant bacteria who often display fitness tradeoffs to avoid death (see Reviews [1-3]). For example, when an ampicillin resistance gene (ampC) containing plasmid that is occasionally found in the wild was transformed into ...


Test-Enhanced Learning In Post-Secondary Biology Courses: The Effect Of Cues And Incentives On High-Level Learning, Bryn Ellen St. Clair Apr 2021

Test-Enhanced Learning In Post-Secondary Biology Courses: The Effect Of Cues And Incentives On High-Level Learning, Bryn Ellen St. Clair

Theses and Dissertations

Cognitive scientists and psychology researchers have given growing attention to evidence of the testing effect, that is, the improvement of students' recall through memory-retrieval practice in the form of quizzes and exams. While laboratory experiments consistently show dramatic positive effects on learning through the testing effect, discipline-specific education researchers have sought to generalize these findings in real, instead of simulated classrooms. The objective of this dissertation was threefold: (1) To survey the current literature on the testing-effect as it applies to learning biology at the post-secondary level. In this review, I consider how further research on the testing effect may ...


Using Geospatial Tools To Assess Changes To Marine Ecosystems In Small Island Developing States Following Hurricane Disturbances: A Case Study Of Dominica After Hurricane Maria, Ryan J. Shields Apr 2021

Using Geospatial Tools To Assess Changes To Marine Ecosystems In Small Island Developing States Following Hurricane Disturbances: A Case Study Of Dominica After Hurricane Maria, Ryan J. Shields

Theses and Dissertations

Seagrass meadows, like coral reefs, are in decline globally but are often neglected in marine policy and conservation despite their equally critical ecosystem services. Both habitats can be heavily impacted by wave surges, rainfall-induced earth movement and flooding, changes to water temperature, salinity, and acidity, and increased levels of turbidity—all occurring at increased rates due to a changing global climate. We demonstrate that multispectral satellite imagery, geospatial tools, and classification techniques can be used to inform management by identifying and quantifying changes in seagrass distribution and the presence of sediment-related threats. Results from Dominica indicate near-shore seagrass habitat area ...


Use Of Flash Flaming Technology To Improve Seed Handling And Delivery Of Winterfat (Krascheninnikovia Lanata), Mitchell Grant Thacker Mar 2021

Use Of Flash Flaming Technology To Improve Seed Handling And Delivery Of Winterfat (Krascheninnikovia Lanata), Mitchell Grant Thacker

Theses and Dissertations

Ecological restoration of rangelands using wild-collected seeds can be challenging due to low seed quality, inconvenient seed anatomy, and poor plant establishment. In North America, the half-shrub winterfat (Krascheninnikovia lanata) is a valuable protein-rich forage for wildlife and livestock. Seeds of this species are contained in one-seeded fruits enclosed in four fluffy, silky bracts. While the seeds can be removed from the bracts, it is not recommended as the bracts are thought to help protect the seed and aid in germination and seedling growth. However, bracts of winterfat make it difficult to incorporate this species within a seed mix because ...


Weekly One-Day Water-Only Fasting Interventional Trial For Low-Density Lipoprotein Cholesterol Reduction (Wonderful), Ciera Lynn Bartholomew Mar 2021

Weekly One-Day Water-Only Fasting Interventional Trial For Low-Density Lipoprotein Cholesterol Reduction (Wonderful), Ciera Lynn Bartholomew

Theses and Dissertations

Purpose: Fasting has been promoted as a method of preventing disease and aging for thousands of years. With heart disease being a leading cause of death in the U.S., researchers have explored the effects of fasting on variables that influence cardiovascular disease (CVD), like LDL cholesterol. Therefore, the purpose of this study was to assess the effects of weekly water-only fasting on LDL cholesterol (LDL-C) in men and women with metabolic risk factors for CVD. Methods: This study was a randomized control trial in adult men and women. Participants were randomized to fasting (treatment) or normal diet (control). The ...


Improving Perennial Bunchgrass Seeding Success In Annual Grass Invaded Areas Using Pre-Emergent Herbicide And Furrowing Techniques, Spencer Chad Camp Mar 2021

Improving Perennial Bunchgrass Seeding Success In Annual Grass Invaded Areas Using Pre-Emergent Herbicide And Furrowing Techniques, Spencer Chad Camp

Theses and Dissertations

Exotic annual weeds have transformed western North America, particularly in sagebrush-steppe systems. Restoration of these invaded sites has been met with low levels of success. Pre-emergent herbicide provides a means to control annual weeds, but typically, this treatment does not allow for the concurrent seeding of desired species. Seeding within a deep, U-shaped furrow following herbicide application may be a method to reduce pre-emergent herbicide effects by transferring the herbicide away from the seed at the time of planting. We tested this potential planting technique by spraying plots with or without the pre-emergent herbicide imazapic, and planting bunchgrass seeds either ...


The Relationship Between Hip Mobility And Performance On The Functional Movement Screen In Apparently Healthy Older Adults: A Preliminary Study, Kimberly Jaye Lindstrom Mar 2021

The Relationship Between Hip Mobility And Performance On The Functional Movement Screen In Apparently Healthy Older Adults: A Preliminary Study, Kimberly Jaye Lindstrom

Theses and Dissertations

Limited or asymmetrical hip mobility can affect movement patterns common to everyday living. The aim of this study was to evaluate the relationship between hip mobility and performance on the Functional Movement Screen (FMS) in apparently healthy older adults. This relationship was evaluated in both males and females. In this observational study, 20 older adults (10 male, 10 female, mean age = 65.65 ± 10.18 years old) completed the Functional Movement Screen and measurements of passive hip range of motion. Separate regression analyses and Pearson Product-Moment Correlations were used to evaluate the relationship between hip mobility measures and FMS composite ...


Pёrcaktimi I Laktozёs Nё Qumёshtin E Disa Kafshёve Vendore, Kaltrina Elezi Feb 2021

Pёrcaktimi I Laktozёs Nё Qumёshtin E Disa Kafshёve Vendore, Kaltrina Elezi

Theses and Dissertations

Laktoza e qumështit shërben si burim kryesor i energjisë tek fëmijët në gji dhe foshnjat si dhe shërben si burim i makromolekulave për funksionimin e sistemit nervor, ushtron efekte të formësimit të mikroflorës së dobishme në zorrë, mbështet funksionin imunitar dhe lehtëson thithjen e mineraleve. Dobitë nga qumështi, veçanësisht produktet nga qumështi siç janë kosi dhe djathi i deleve dhe dhive janë të padiskutuara për njerëzit e moshave të rritura deri në pleqëri, veçanërisht për personat me probleme të keq tretjes së laktozës, pasi që këto produkte nuk përmbajnë ose përmbajnë fare pak laktozë.

Duke hipotezuar që për prodhimin e ...


Roli I Kripës Dhe Roli I Pjekjes Në Bukën Artizanale, Donjeta Bajrami Feb 2021

Roli I Kripës Dhe Roli I Pjekjes Në Bukën Artizanale, Donjeta Bajrami

Theses and Dissertations

Konsumimi i produkteve me prejardhje mielli në vendin tonë ka pasur gjithmon interesim të madh, ku me kalimin e viteve kjo fushë është zgjeruar shumë duke futur në treg produkte të reja konditore. Buka artizanale me brum të thartë, është bukë e cila prodhohet me maja natyrale e bërë paraprakisht të paktën një javë para përdorimit dhe që ka një mënyrë të veqant të pjekjes. Buka artizanale njihet për vlerat ushqyese, andaj edhe qëllimi i këtij punimi ka qenë detektimi i furrave të cilat ende prodhojnë bukë artizane në komunën e Prishtinës dhe të përcaktohen analizat organoshqisore, fiziko-kimike, NaCl, poroziteti ...


Ndikimi I Proceseve Termike Nё Produktin Pёrfudimtar Tё Lёngut Tё Mollёs, Arlinda Kqiku Feb 2021

Ndikimi I Proceseve Termike Nё Produktin Pёrfudimtar Tё Lёngut Tё Mollёs, Arlinda Kqiku

Theses and Dissertations

Cilësia e produktit dhe shqetësimi i sigurisë së ushqimit ka promovuar zhvillimin e metodave të ndryshme të përpunimit termik ku aktivitetet biologjike dhe kimike duhet të vihen nën kontroll dhe kjo zakonisht sigurohet nga përpunimi tradicional termik për të prodhuar lëng perfudimtar të mollës. Sidoqoftë, lartësia e temperaturës së aplikuar ka një ndikim negativ në parametrat e rëndësishëm të cilat janë: ngjyra e ushqimit, vlera ushqyese, karakteristikat ndijore dhe aroma. Prandaj, ky studim ka për qëllim, angazhimin në përpjekjen për të marrë parametrat e cilësisë dhe vlerat ushqyese të produktit të lëngut të mollës me më pak devijim. Për këtë ...


Mining Selected Metagenomes/Metatranscriptomes For Biosynthetic Gene Clusters And Antimicrobial Resistance Genes, Ahmed Yamany Jan 2021

Mining Selected Metagenomes/Metatranscriptomes For Biosynthetic Gene Clusters And Antimicrobial Resistance Genes, Ahmed Yamany

Theses and Dissertations

Antimicrobial resistance is one of the serious global challenges in the current century. The fact that resistance genes transfer between bacteria, coupled with the fact that the world is connected through complex dynamics. Studying microbial behavior and understanding the different factors coffering microbial resistance to a broad spectrum of the available drug classes, parallel with a comprehensive analysis of the natural microbial products as the primary source of the novel antibiotics, might shed some light on solutions for this problem. Microbial environments harbor a wide range of secondary metabolites (SM) with different functional groups. SMs are not directly involved in ...


Egyptian Women’S Agriculture Contribution; Assessment Of The Gender Gap For Sustainable Development, Noha El Khorazaty Jan 2021

Egyptian Women’S Agriculture Contribution; Assessment Of The Gender Gap For Sustainable Development, Noha El Khorazaty

Theses and Dissertations

Women’s contribution to the agriculture sector in developing countries is undeniable, yet they do not have equal access to the resources and opportunities they need to be more productive. Sustainable development entails inclusive and effective management of natural resources, this entails gender equity in agriculture. Bridging the gender gap in agriculture far exceeds the benefits of the individual. According to the latest estimates bridging the yield gap in agricultural productivity could possibly decrease the numbers of undernourished people in the world by around 100 – 140 million people. Sustainable agriculture development and gender equity necessitate policy interventions targeting the gender ...


Prolactin Drives A Dynamic Stat5a/Hdac6/Hmgn2 Cis-Regulatory Landscape Exploitable In Er+ Breast Cancer, Justin M. Craig Jan 2021

Prolactin Drives A Dynamic Stat5a/Hdac6/Hmgn2 Cis-Regulatory Landscape Exploitable In Er+ Breast Cancer, Justin M. Craig

Theses and Dissertations

The hormone prolactin has been implicated in breast cancer pathogenesis and regulates chromatin engagement by the transcription factor, STAT5A. STAT5A is known to inducibly bind promoters and cis-regulatory elements genome-wide, though the mechanisms by which it exerts specificity and regulation of target gene expression remain enigmatic. We previously identified HDAC6 and HMGN2 as cofactors that facilitate prolactin induced, STAT5A mediated gene expression. Here, multi-condition STAT5A, HDAC6, and HMGN2 ChIP-seq with parallel condition RNA-seq are utilized to reveal the cis-regulatory landscape and cofactor dynamics underlying prolactin stimulated gene expression in breast cancer. We find that prolactin regulated genes are ...


Parametrat Mikrobilogjik Të Djathit Në Rajonin E Prizrenit, Valentina Dani Jan 2021

Parametrat Mikrobilogjik Të Djathit Në Rajonin E Prizrenit, Valentina Dani

Theses and Dissertations

Djathi është një nga ushqimet kryesore në Republikën e Kosovës. Mikrooganizmat patogjen (Salmonella dhe Listeria monocytogenes) shumë lehtë mund të kontaminojnë dhe rikontaminojnë djathin nëse nuk zbatohen të gjitha masat e sigurisë gjatë gjithë zinxhirit ushqimor. Në këtë studim janë përzgjedhur për tu hulumtuar dy mikroorganizma që kanë patogjenitet të lartë (Salmonella dhe Listeria monocytogenes), nëse këta gjenden në ushqim kanë shkallë të lartë patogjeniteti, për rrjedhoj dëmtojnë konsumatorin dhe prodhuesin duke humbur besimin e konsumatorit. Mostrat janë marrë në periudha të ndryshme kohore dhe fatmirësisht në asnjë mostër nga pesë njësi mostra të anlizuar nuk janë gjetur Salmonella dhe ...


Vlerёsimi I Cilёsisё Nё Prodhimin E Chipsit “Vipa Chips”, Elbasane Sadiku Jan 2021

Vlerёsimi I Cilёsisё Nё Prodhimin E Chipsit “Vipa Chips”, Elbasane Sadiku

Theses and Dissertations

Patatja (Solanum tuberosum L.) është kultura e katërt më e rëndësishme e perimeve në botë si një kulturë ushqimore për konsum njerëzor. Patatet e kultivuara janë përhapur nga Amerika e Jugut ku kanë origjinën në 160 vende të botës. Konsumi i patateve të freskëta ka rënë ndërsa produktet e përpunuara të patates janë rritur në popullaritet. Ndërsa patatja bëhet element kryesor në dietat e e numrit në rritje të njerëzve, ndryshimet e vogla në përbërjen ushqyese të patates do të kenë ndikime të mëdha në shëndetin e popullatës. Patatja është një ushqim i pasur me karbohidrate, që siguron energji me ...


Vlerësimi I Parametrave Fiziko-Kimik Të Qumështit Në Disa Pika Grumbulluese Në Komunën E Sharrit, Alban Halimi Jan 2021

Vlerësimi I Parametrave Fiziko-Kimik Të Qumështit Në Disa Pika Grumbulluese Në Komunën E Sharrit, Alban Halimi

Theses and Dissertations

Qumështi i cili prodhohet në fermat e qumështores Sharri është i kompletuar si në aspektin fizik, kimik ashtu dhe në aspektin organoleptik. Parametrat fiziko-kimik të qumështit kanë rëndësi të madhe në procesin e përpunimit të qumështit. Kontrolli i këtyre parametrave është pjësë e sigurisë ushqimore dhe përfshin proces tekonologjik si dhe lëndë shtesë të aprovuara nga standardi shtetëror. Me anë të paisjeve moderne kam hulumtuar në 4 pikat grumbulluese të qumështores Sharri. Për vlerësimin e këtyre parametrave kam kaluar një eksperiencë mjaftë të mirë dhe shumë të suksesshme në këtë qumështore me paisje dhe me anë të metodave të reja ...


Problemet Në Rritje Dhe Zhvillim Të Fëmijëve Në Institucionet Parashkollore Si Pasojë E Ushqyeshmërisë Jo Adekuate, Agon Begaj Jan 2021

Problemet Në Rritje Dhe Zhvillim Të Fëmijëve Në Institucionet Parashkollore Si Pasojë E Ushqyeshmërisë Jo Adekuate, Agon Begaj

Theses and Dissertations

Kequshqyerja tek fëmijët mund të shkaktojë zhvillim dhe rritje të dobët. Ushqyeshmëria adekuate është kyçe për shëndetin dhe zhvillimin e çdo gjenerate. Qëllimi i këtij hulumtimi është për të vlerësuar nivelin e nën-ushqyerjes dhe kequshqyerjes te fëmijët nën moshën 6 vjeçare në Republikën e Kosovës. Hulumtimi është kryer duke marrë për mostër 200 fëmijë në institucionet parashkollore publike dhe private. Instrumentet e përdorura për kryerjen e hulumtimit ishin: pyetësori socio-demografikë, matjet antropometrike duke përfshirë peshë dhe gjatësi, si dhe fleta kontrolluese për zhvillimin e fëmijëve. Rezultatet e arritura treguan se 59% të respodentëve kanë rritje dhe zhvillim mesatar, 20% ishin ...


Identification And Optimization Of Mirna Biomarkers For Body Mass Index (Bmi) Estimation In Forensic Samples, Jennifer J. Shealy Jan 2021

Identification And Optimization Of Mirna Biomarkers For Body Mass Index (Bmi) Estimation In Forensic Samples, Jennifer J. Shealy

Theses and Dissertations

DNA profiles do not provide as much evidentiary value when either all known samples can be excluded or when there is no known suspect. DNA phenotyping is a technique used for investigative purposes to predict externally visible characteristics (EVCs) based on specific markers found in the genome. Most phenotyping panels are currently restricted to markers stable over a lifetime within an individual and are not able to predict environmental or metabolic impacts on EVCs. It is known that miRNA expression levels change due to environmental and metabolic factors, such as BMI, and research has proven associations between circulating plasma miRNAs ...


Pushing The Limits: Increasing The Speed And Specificity Of Sars-Cov-2 Testing, Grayson Way Jan 2021

Pushing The Limits: Increasing The Speed And Specificity Of Sars-Cov-2 Testing, Grayson Way

Theses and Dissertations

The prevalence and spread of the current COVID-19 pandemic have highlighted the importance of continual improvements upon current microbiological testing methods. Rapid and accurate testing can help mitigate spread by improving on the time to quarantine and quarantine duration required. As of the writing of this thesis, COVID-19 has been responsible for more than 500,000 deaths in the United States of America, and greater than 2 million deaths globally. The work done in this thesis has shown improvements in the current SARS-CoV-2 testing methodology by reducing the time it takes for patient testing while maintaining accuracy and the sensitivity ...


Single Molecule Investigations Of Holliday Junction Binding Protein Ruva, Dalton Reed Gibbs Jan 2021

Single Molecule Investigations Of Holliday Junction Binding Protein Ruva, Dalton Reed Gibbs

Theses and Dissertations

DNA breaks are inevitable as they mainly occur due to cells’ own reactive oxygen species (ROS). While DNA breaks can be single-stranded or double-stranded, the double-stranded DNA (dsDNA) breaks are more dangerous. If such damage is not repaired, it can lead to genetic instability and serious health issues including cancers. One way dsDNA breaks can be repaired is via a process called homologous recombination (HR), which involves several DNA-binding proteins. Therefore, to have a better insight into the repair mechanism and origin of repair defects, we need a better understanding of how these proteins interact with DNA itself and DNA ...


Mathematical Modeling Of Lung Inflammation: Macrophage Polarization And Ventilator-Induced Lung Injury With Methods For Predicting Outcome, Sarah B. Minucci Jan 2021

Mathematical Modeling Of Lung Inflammation: Macrophage Polarization And Ventilator-Induced Lung Injury With Methods For Predicting Outcome, Sarah B. Minucci

Theses and Dissertations

Lung insults, such as respiratory infections and lung injuries, can damage the pulmonary epithelium, with the most severe cases needing mechanical ventilation for effective breathing and survival. Furthermore, despite the benefits of mechanical ventilators, prolonged or misuse of ventilators may lead to ventilation-associated/ventilation-induced lung injury (VILI). Damaged epithelial cells within the alveoli trigger a local immune response. A key immune cell is the macrophage, which can differentiate into a spectrum of phenotypes ranging from pro- to anti-inflammatory. To gain a greater understanding of the mechanisms of the immune response in the lungs and possible outcomes, we developed several mathematical ...


Qualitative And Quantitative Characterization Of Epithelial Cell Mixtures Using Hormone Antibody Probes And Flow Cytometry, Luke Baldridge Jan 2021

Qualitative And Quantitative Characterization Of Epithelial Cell Mixtures Using Hormone Antibody Probes And Flow Cytometry, Luke Baldridge

Theses and Dissertations

“Touch” DNA is evidence that consists of epidermal cells deposited by handling objects and it is becoming more common in evidence samples processed in crime labs. Because “touch” DNA evidence samples may be low-template DNA and are often mixtures, there is a need to both nondestructively estimate the amount of DNA present prior to DNA purification and to identify and characterize mixtures prior to DNA typing. The purpose of this study was to test the use of FITC-labeled anti-testosterone antibodies as a potential tool to estimate the number of contributors in two-, three-, and four-person mixtures of epidermal skin cells ...


Phenotypic Characterization Of Table Mountain (Pinus Pungens) And Pitch Pine (Pinus Rigida) Hybrids Along An Elevational Gradient In The Blue Ridge Mountains, Virginia, Alexander L. Brown Jan 2021

Phenotypic Characterization Of Table Mountain (Pinus Pungens) And Pitch Pine (Pinus Rigida) Hybrids Along An Elevational Gradient In The Blue Ridge Mountains, Virginia, Alexander L. Brown

Theses and Dissertations

Hybridization has played a long-standing role in the evolution of both plant and animal species and allows for the sharing of genetic information between lineages. Here, potential hybridization of a species endemic to the Appalachian Mountains, Table Mountain pine (Pinus pungens), and pitch pine (Pinus rigida) was investigated along an elevational gradient, through the use of phenotypic measurements: cone length, cone width, and needle length. Phenotypes were used to identify hybrids in a three-tiered elevational sampling method at two sites in Shenandoah National Park with the use of linear discriminant analysis. It was found that hybridization between Table Mountain and ...


Searching For Gold: Using A Novel Land Cover Classification To Identify Multiscale Drivers Of Site Occupancy By A Flagship Species For Early-Successional Habitat Conservation, Baron Lin Jan 2021

Searching For Gold: Using A Novel Land Cover Classification To Identify Multiscale Drivers Of Site Occupancy By A Flagship Species For Early-Successional Habitat Conservation, Baron Lin

Theses and Dissertations

Understanding habitat selection at multiple scales is an important step in guiding conservation programs and reversing species declines. This, however, is difficult for species that occupy early-successional habitats (ESH) due to a lack of accurate representation of shrub cover in publicly available land cover data. The Golden-winged Warbler (Vermivora chrysoptera; GWWA) is a threatened species of conservation concern and a flagship for ESH conservation. We used a novel, LIDAR-derived land cover classification that accurately identifies shrubs at a fine resolution (1m) to investigate how habitat composition and configuration influence GWWA site occupancy. We aggregated this same land cover data to ...


Crop Response To Low-Dose Dicamba, Mason Castner Jan 2021

Crop Response To Low-Dose Dicamba, Mason Castner

Theses and Dissertations

The introduction of dicamba-resistant (DR) soybean [Glycine max (L.) Merr.] and cotton (Gossypium hirsutum L.) followed by a label allowing over-the-top applications of dicamba in the 2017 provided growers an additional option for broadleaf weed control. Because non-DR soybean is sensitive to low concentrations of dicamba, postemergence dicamba applications present growers choosing to plant non-DR soybean with concerns of damage from off-target movement through physical drift and volatilization of the herbicide as well as tank-contamination from prior dicamba application. Consequences of low-dose dicamba exposure on sensitive vegetative and reproductive soybean have been well researched, but little is known regarding the ...


Water Clarity At The River-Estuary Transition Zone: A Comparative Study Of The James, Mattaponi, And Pamunkey Sub-Estuaries, Rachel Henderson Jan 2021

Water Clarity At The River-Estuary Transition Zone: A Comparative Study Of The James, Mattaponi, And Pamunkey Sub-Estuaries, Rachel Henderson

Theses and Dissertations

Water clarity is a key parameter for monitoring water quality and often used to assess habitat suitability for submerged aquatic vegetation (SAV). Light attenuation, a measure of water clarity, is impacted by colored dissolved organic matter (CDOM), and by suspended particulates which include living and non-living components. We anticipated that the relative importance of these factors in regulating light attenuation would vary among the upper portions of three sub-estuaries differing in morphometry, hydrology, and degree of human influence. The James is characterized by eutrophic conditions and high algal abundance, whereas the Mattaponi and Pamunkey exhibit lower phytoplankton production. The Mattaponi ...


The Role Of Autophagy And Senescence In The Responses Of Non-Small Cell Lung Cancer Cells To Chemotherapy And Radiation, Nipa H. Patel Jan 2021

The Role Of Autophagy And Senescence In The Responses Of Non-Small Cell Lung Cancer Cells To Chemotherapy And Radiation, Nipa H. Patel

Theses and Dissertations

Cancer-associated deaths account for the second-highest mortality rates in the United States. Primary modalities of treatment often include surgery, radiation, and chemotherapy, and may also incorporate targeted therapy and immunotherapy. However, resistance to these treatments remains high, resulting in disease reoccurrence and poor survival rates. While apoptosis or cell death of tumor cells is the ideal outcome for anti-cancer therapy, this is often not the case, and in fact cancer cells may upregulate several pathways, such as autophagy and senescence, as a means to undergo alternative cell fate and evade apoptotic cell death. An essential tumor suppressor gene, TP53, regulates ...


Radioimmunoconjugate For Cancer Molecular Imaging, Nouri Elmekharam Jan 2021

Radioimmunoconjugate For Cancer Molecular Imaging, Nouri Elmekharam

Theses and Dissertations

Over expression of Sperm Acrosomal SLLP1 Binding protein (SAS1B) on the surface of uterine cancer and the availability of different anti-SAS1B monoclonal antibodies (mAbs) that specifically bind to it, offered an opportunity to label these antibodies with radionuclides for cancer imaging. Although optimizing in vivo stable radiolabeling is challenging, imaging this type of aggressive cancer will improve the treatment strategy. The objective of this work is to develop a radiolabeled anti-SAS1B antibodies with Zircunum-89 (89Zr) and Copper-67 (67Cu) and determine, in vitro and in vivo their uptake and biodistribution. Two anti-SAS1B monoclonal antibodies (SB2, and SB5) were labeled ...


A Mechanism Behind The Mechanotransduction Of Surface Characteristics In Osteoblasts, Otto J. Juhl Iv Jan 2021

A Mechanism Behind The Mechanotransduction Of Surface Characteristics In Osteoblasts, Otto J. Juhl Iv

Theses and Dissertations

Biomaterials for use in bone regeneration and healing range from metal and metal alloy implants to hydrogel-based solutions. These materials can be optimized to increase bone healing and integration by improving the mechanical and biological properties. Regardless of the material itself, the cell-substrate interaction is key to the success of the biomaterial once implanted. Substrate surface characteristics such as roughness, wettability, and particle density are well-known contributors to a substrate’s overall osteogenic potential, and therefore the substrate's overall success. Unfortunately, it is still unknown how these substrate surface characteristics are transduced into intracellular signals by cells, preventing specific ...