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Full-Text Articles in Life Sciences

An Orange Fluorescent Protein Tagging System For Real-Time Pollen Tracking, J. Rice, R. Mundell, R. Millwood, O. Chambers, L. Abercrombie, H. Davies, C. Stewart Dec 2102

An Orange Fluorescent Protein Tagging System For Real-Time Pollen Tracking, J. Rice, R. Mundell, R. Millwood, O. Chambers, L. Abercrombie, H. Davies, C. Stewart

C. Neal Stewart Jr.

Background Monitoring gene flow could be important for future transgenic crops, such as those producing plant-made-pharmaceuticals (PMPs) in open field production. A Nicotiana hybrid (Nicotiana. tabacum × Nicotiana glauca) shows limited male fertility and could be used as a bioconfined PMP platform. Effective assessment of gene flow from these plants is augmented with methods that utilize fluorescent proteins for transgenic pollen identification. Results We report the generation of a pollen tagging system utilizing an orange fluorescent protein to monitor pollen flow and as a visual assessment of transgene zygosity of the parent plant. This system was created to generate a tagged ...


Sedimentary Processes And Products Of Ephemeral Acid Saline Lakes In Southern Western Australia, Francisca Oboh-Ikuenobe, Elliot A. Jagniecki, D. A. Laclair, Stacy L. Story, Melanie R. Mormile, B. Hong, Kathleen C. Benison, Brenda Beitler Bowen Nov 2017

Sedimentary Processes And Products Of Ephemeral Acid Saline Lakes In Southern Western Australia, Francisca Oboh-Ikuenobe, Elliot A. Jagniecki, D. A. Laclair, Stacy L. Story, Melanie R. Mormile, B. Hong, Kathleen C. Benison, Brenda Beitler Bowen

Francisca Oboh-Ikuenobe

No abstract provided.


In-House Daily Consensus Conference: An Important Quality Control/Quality Assurance Activity--Experience At A Major Referral Center., Amna Khurshid, Zubair Ahmad, Asim Qureshi May 2017

In-House Daily Consensus Conference: An Important Quality Control/Quality Assurance Activity--Experience At A Major Referral Center., Amna Khurshid, Zubair Ahmad, Asim Qureshi

Zubair Ahmad

Background:For every practicing histopathologist, improvement of diagnostic accuracy is an important objective. Personal consults are an important component of quality control (QC)/quality assurance (QA) in our Section of Histopathology. In addition, the College of American Pathologists recommends a daily in-house consensus conference, which is a prospective system by which all difficult and problematic cases are reviewed and discussed and signed out by consensus. Design: In-house consensus conference is held daily using a multi-headed microscope. This collegial session is run by the seniormost consultant in the section and is attended by all histopathology consultants and residents. The consultants and ...


Visual Methods For Collaborative Food System Work, K. Valentine Cadieux, Charles Levkoe, P. Mount, D. Szanto Dec 2016

Visual Methods For Collaborative Food System Work, K. Valentine Cadieux, Charles Levkoe, P. Mount, D. Szanto

K. Valentine Cadieux

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Reactive Biliary Epithelium: The Product Of A Pluripotential Stem Cell Compartment?, Matthew Golding, Catherine Sarraf, El-Nasir Lalani, Malcolm R. Alison Aug 2016

Reactive Biliary Epithelium: The Product Of A Pluripotential Stem Cell Compartment?, Matthew Golding, Catherine Sarraf, El-Nasir Lalani, Malcolm R. Alison

El Nasir Lalani

Liver parenchymal cells (hepatocytes) have a low rate of turnover, but can nevertheless mount a rapid and efficient regenerative response. However, in some cases of extreme hepatotoxicity hepatocyte proliferation is restricted or even abolished, and instead biliary epithelial cells, commonly referred to as ductular oval cells, migrate into the periportal and midzonal parenchyma. Initially these cells behave as authentic biliary epithelium with expression of the biliary cytokeratin intermediate filaments, but then show hepatocytic traits such as alpha fetoprotein and albumin synthesis. Thereafter these biliary ducts rapidly vanish to be replaced by either small hepatocytes or intestinal-type cells. The proliferation and ...


Muc1: A Genetic Susceptibility To Infertility?, Andrew W. Horne, John O. White, Raul A. Margara, Ross Williams, Robert Ml Winston, El-Nasir Lalani Aug 2016

Muc1: A Genetic Susceptibility To Infertility?, Andrew W. Horne, John O. White, Raul A. Margara, Ross Williams, Robert Ml Winston, El-Nasir Lalani

El Nasir Lalani

No abstract provided.


Muc1 In Normal And Impaired Spermatogenesis, Folker E. Franke, Sigurd Kraus, Claus Eiermann, Katharina Pauls, El-Nasir Lalani, Martin Bergmann Aug 2016

Muc1 In Normal And Impaired Spermatogenesis, Folker E. Franke, Sigurd Kraus, Claus Eiermann, Katharina Pauls, El-Nasir Lalani, Martin Bergmann

El Nasir Lalani

The MUC1 mucin [also known as episialin, epithelial membrane antigen (EMA) or polymorphic epithelial mucin (PEM)] is a component of the mucosal glycocalyx, contributing to anti-adhesive and protective cell functions. MUC1 has been shown in a variety of epithelial cell types in the reproductive tracts of males and females, but this is the first report of its expression in human testis and non-epithelial cells of the germ cell lineage. Analysing 65 testes with normal or impaired spermatogenesis, we identified MUC1 protein in maturing germ cells by immunohistochemistry using the monoclonal antibodies HMFG1, HMFG2 and SM3 binding to different glycosylation variants ...


Muc1 Expressed In Panc1 Cells Decreases Adhesion To Type 1 Collagen But Increases Contraction In Collagen Lattices, M J.H Hudson, G W.H Stamp, M A. Hollingsworth, M Pignatelli, El-Nasir Lalani Aug 2016

Muc1 Expressed In Panc1 Cells Decreases Adhesion To Type 1 Collagen But Increases Contraction In Collagen Lattices, M J.H Hudson, G W.H Stamp, M A. Hollingsworth, M Pignatelli, El-Nasir Lalani

El Nasir Lalani

A subline of human pancreatic cells (PanC1) that expresses low levels of cytokeratins 8 and 18 but not MUC1 mucin was transfected with both 3.5-kb and 3.9-kb full-length MUC1 cDNA. The MUC1-positive clone expressing the larger mucin was shown to express increased levels of cytokeratins 8 and 18 compared with the parental line or vector controls. Growth of these MUC1-transfected cells in type I collagen gels produced marked gel contraction that could be significantly reduced by the synthetic peptide SRGDTG or by growth in serum and fibronectin-depleted media. Cellular binding to type I collagen was found to be ...


Human Muc1 Mucin: A Potent Glandular Morphogen, Mark J. Hudson, Gordon W. Stamp, Khurram S. Chaudhary, Robert Hewitt, Andrew P. Stubbs, Paul D. Abel, El-Nasir Lalani Aug 2016

Human Muc1 Mucin: A Potent Glandular Morphogen, Mark J. Hudson, Gordon W. Stamp, Khurram S. Chaudhary, Robert Hewitt, Andrew P. Stubbs, Paul D. Abel, El-Nasir Lalani

El Nasir Lalani

Human MUC1 mucin is a high-molecular-weight transmembrane glycoprotein, which is apically expressed in the majority of glandular epithelia. During embryonic development, changes in the pattern of MUC1 mucin expression coincide with the onset of glandular differentiation. This mucin is also frequently overexpressed and aberrantly glycosylated in carcinomas. To investigate the potential role of MUC1 mucin in morphogenesis, a full length MUC1 cDNA was transfected into murine mammary adenocarcinoma (410.4) and Madin-Darby canine kidney (MDCK) cells. This generated four clonal cell lines. Western blotting, FACS analysis, and immunohistochemistry confirmed expression of MUC1. All four MUC1-expressing clones demonstrated altered morphogenesis when ...


Corn And Soybean Production With A Winter Rye Cover Crop, John E. Sawyer, Jose L. Pantoja, Daniel W. Barker Jul 2016

Corn And Soybean Production With A Winter Rye Cover Crop, John E. Sawyer, Jose L. Pantoja, Daniel W. Barker

John E. Sawyer

Objectives of this project were to study corn nitrogen (N) fertilization requirement and corn/soybean yield response when grown in a rye cover cropping system. Multiple rates of N fertilizer were applied, with measurement of corn yield response to applied N and soybean yield with and without a fall planted winter rye cover crop. The study was conducted at multiple research farms, with the intent for comparison of with and without a cover crop system across varying soil and climatic conditions in Iowa.


Corn And Soybean Production With A Winter Rye Cover Crop, John E. Sawyer, Jose L. Pantoja, Daniel W. Barker Jul 2016

Corn And Soybean Production With A Winter Rye Cover Crop, John E. Sawyer, Jose L. Pantoja, Daniel W. Barker

John E. Sawyer

Objectives of this project were to study corn nitrogen (N) fertilization requirement and corn/soybean yield response when grown in a rye cover cropping system. Multiple rates of N fertilizer were applied, with measurement of corn yield response to applied N and soybean yield with and without a fall planted winter rye cover crop. The study was conducted at multiple research farms, with the intent for comparison of with and without a cover crop system across varying soil and climatic conditions in Iowa.


Characterization Of Ceriporiopsis Subvermispora Bicupin Oxalate Oxidase Expressed In Pichia Pastoris, Patricia Moussatche, Eric Hoffer, Ellen W. Moomaw, Et Al. Jun 2016

Characterization Of Ceriporiopsis Subvermispora Bicupin Oxalate Oxidase Expressed In Pichia Pastoris, Patricia Moussatche, Eric Hoffer, Ellen W. Moomaw, Et Al.

Ellen Moomaw

Oxalate oxidase (E.C. 1.2.3.4) catalyzes the oxygen-dependent oxidation of oxalate to carbon dioxide in a reaction that is coupled with the formation of hydrogen peroxide. Although there is currently no structural information available for oxalate oxidase from Ceriporiopsis subvermispora (CsOxOx), sequence data and homology modeling indicate that it is the first manganese-containing bicupin enzyme identified that catalyzes this reaction. Interestingly, CsOxOx shares greatest sequence homology with bicupin microbial oxalate decarboxylases (OxDC). We show that CsOxOx activity directly correlates with Mn content and other metals do not appear to be able to support catalysis. EPR spectra indicate ...


Fungal Oxalate Decarboxylase Activity Contributes To Sclerotinia Sclerotiorum Early Infection By Affecting Both Compound Appressoria Development And Function, Xiaofei Liang, Ellen W. Moomaw, Jeffrey A. Rollins Jun 2016

Fungal Oxalate Decarboxylase Activity Contributes To Sclerotinia Sclerotiorum Early Infection By Affecting Both Compound Appressoria Development And Function, Xiaofei Liang, Ellen W. Moomaw, Jeffrey A. Rollins

Ellen Moomaw

Sclerotinia sclerotiorum pathogenesis requires the accumulation of high levels of oxalic acid (OA). To better understand the factors affecting OA accumulation, two putative oxalate decarboxylase (OxDC) genes (Ss-odc1 and Ss-odc2) were characterized. Ss-odc1 transcripts exhibited significant accumulation in vegetative hyphae, apothecia, early stages of compound appressorium development and during plant colonization. Ss-odc2 transcripts, in contrast, accumulated significantly only during mid to late stages of compound appressorium development. Neither gene was induced by low pH or exogenous OA in vegetative hyphae. A loss-of-function mutant for Ss-odc1 (Δss-odc1) showed wild-type growth, morphogenesis and virulence, and was not characterized further. Δss-odc2 mutants hyperaccumulated ...


Characterization Of Ceriporiopsis Subvermispora Bicupin Oxalate Oxidase Expressed In Pichia Pastoris, Patricia Moussatche, Eric Hoffer, Ellen W. Moomaw, Et Al. Jun 2016

Characterization Of Ceriporiopsis Subvermispora Bicupin Oxalate Oxidase Expressed In Pichia Pastoris, Patricia Moussatche, Eric Hoffer, Ellen W. Moomaw, Et Al.

Ellen Moomaw

Oxalate oxidase (E.C. 1.2.3.4) catalyzes the oxygen-dependent oxidation of oxalate to carbon dioxide in a reaction that is coupled with the formation of hydrogen peroxide. Although there is currently no structural information available for oxalate oxidase from Ceriporiopsis subvermispora (CsOxOx), sequence data and homology modeling indicate that it is the first manganese-containing bicupin enzyme identified that catalyzes this reaction. Interestingly, CsOxOx shares greatest sequence homology with bicupin microbial oxalate decarboxylases (OxDC). We show that CsOxOx activity directly correlates with Mn content and other metals do not appear to be able to support catalysis. EPR spectra indicate ...


Fungal Oxalate Decarboxylase Activity Contributes To Sclerotinia Sclerotiorum Early Infection By Affecting Both Compound Appressoria Development And Function, Xiaofei Liang, Ellen W. Moomaw, Jeffrey A. Rollins Jun 2016

Fungal Oxalate Decarboxylase Activity Contributes To Sclerotinia Sclerotiorum Early Infection By Affecting Both Compound Appressoria Development And Function, Xiaofei Liang, Ellen W. Moomaw, Jeffrey A. Rollins

Ellen Moomaw

Sclerotinia sclerotiorum pathogenesis requires the accumulation of high levels of oxalic acid (OA). To better understand the factors affecting OA accumulation, two putative oxalate decarboxylase (OxDC) genes (Ss-odc1 and Ss-odc2) were characterized. Ss-odc1 transcripts exhibited significant accumulation in vegetative hyphae, apothecia, early stages of compound appressorium development and during plant colonization. Ss-odc2 transcripts, in contrast, accumulated significantly only during mid to late stages of compound appressorium development. Neither gene was induced by low pH or exogenous OA in vegetative hyphae. A loss-of-function mutant for Ss-odc1 (Δss-odc1) showed wild-type growth, morphogenesis and virulence, and was not characterized further. Δss-odc2 mutants hyperaccumulated ...


A Level Of Discomfort! Exploring The Relationship Between Maternal Sexual Health Knowledge, Religiosity And Comfort Discussing Sexual Health Issues With Adolescents, Fiona Farringdon, Courtney Holgate, Fleur Mcintyre, Max Bulsara May 2016

A Level Of Discomfort! Exploring The Relationship Between Maternal Sexual Health Knowledge, Religiosity And Comfort Discussing Sexual Health Issues With Adolescents, Fiona Farringdon, Courtney Holgate, Fleur Mcintyre, Max Bulsara

Fleur McIntyre

This pilot study explored if a relationship existed between maternal sexual health knowledge, religiosity and comfort discussing sexual health issues with adolescents. Seventy-six mothers with adolescent children aged between 10 and 19 completed a combined survey addressing religiosity, sexual health knowledge and comfort discussing sexual health issues. Mann–Whitney U tests were performed to compare the median scores, Spearman’s rho tests were performed to determine correlations between the median scores, Fisher’s exact tests were computed to test for significant differences between proportions and a logistic regression model was used to investigate significant factors. No significant differences or relationships ...


Reef Fish Assemblage Structure Variability Off Southeast Florida: Ongoing Research, R. Navarra, Aarti Raja May 2016

Reef Fish Assemblage Structure Variability Off Southeast Florida: Ongoing Research, R. Navarra, Aarti Raja

Aarti Raja

No abstract provided.


Integration Of Scientific Concepts Through A Hands-On Learning Program: Science Alive!, T. Gali, Aarti Raja May 2016

Integration Of Scientific Concepts Through A Hands-On Learning Program: Science Alive!, T. Gali, Aarti Raja

Aarti Raja

No abstract provided.


Telithromycin. Fresh From The Pipeline, Aarti Raja, J. Lebbos, P. Kirtpatrick May 2016

Telithromycin. Fresh From The Pipeline, Aarti Raja, J. Lebbos, P. Kirtpatrick

Aarti Raja

In April 2004, telithromycin (Ketek; Aventis), the first of a new class of antibacterial drugs derived from macrolides, was approved by the US FDA for the treatment of several respiratory-tract infections. After a delay in its approval owing to a need to address potential safety issues, is it likely to now attain the blockbuster status that had originally been widely predicted?


Fcas Service Learning Projects Winter Semester 2013, Aarti Raja, Robert P. Smith, L. Vigesaa May 2016

Fcas Service Learning Projects Winter Semester 2013, Aarti Raja, Robert P. Smith, L. Vigesaa

Aarti Raja

No abstract provided.


Interaction Of Hiv Reverse Transcriptase With Structures Mimicking Recombination Intermediates, Aarti Raja, J. Jeffrey, J. J. Destefano May 2016

Interaction Of Hiv Reverse Transcriptase With Structures Mimicking Recombination Intermediates, Aarti Raja, J. Jeffrey, J. J. Destefano

Aarti Raja

Interactions between human immunodeficiency virus (HIV) reverse transcriptase (RT) and structures mimicking intermediates proposed to occur during recombination (strand transfer) were investigated. One mechanism proposed for strand transfer is strand exchange in which a homologous RNA (acceptor) "invades" a donor RNA.DNA duplex (replication intermediate) on which DNA synthesis is occurring. The acceptor displaces the donor of the duplex and binds to the DNA. During exchange a transient trimeric structure forms. A model structure was designed with a replication intermediate to which an acceptor RNA was bound. The acceptor was bound to the 5'-end of the DNA over a ...


In Vitro Strand Transfer From Broken Rnas Results In Mismatch But Not Frameshift Mutations, Jeffrey J. Destefano, Aarti Raja, Jason V. Christofaro May 2016

In Vitro Strand Transfer From Broken Rnas Results In Mismatch But Not Frameshift Mutations, Jeffrey J. Destefano, Aarti Raja, Jason V. Christofaro

Aarti Raja

An in vitro system to compare the fidelity of strand transfers from truncated vs full-length RNAs was constructed. A donor RNA, on which reverse transcriptase (RT)-directed DNA synthesis was initiated, shared homology with an acceptor RNA, to which DNAs initiated on the donor could transfer. All RNAs were derived from the N-terminal portion of the alpha-lac gene. On full-length donors, transfers occurred when DNAs migrated to the acceptor prior to being completed on the donor. On donors that were truncated, most transfers occurred after DNAs reached the end of the donor. Transfer products were amplified by PCR and used ...


Biology On The Go – Science Outreach For K To 12 And Beyond!, A. William, Aarti Raja May 2016

Biology On The Go – Science Outreach For K To 12 And Beyond!, A. William, Aarti Raja

Aarti Raja

No abstract provided.


Empowerment Of Young People Who Have A Parent Living With Dementia: A Social Model Perspective, Karen Hutchinson, Chris Roberts, Michele Daly, Caroline Bulsara, Susan Kurrle May 2016

Empowerment Of Young People Who Have A Parent Living With Dementia: A Social Model Perspective, Karen Hutchinson, Chris Roberts, Michele Daly, Caroline Bulsara, Susan Kurrle

Caroline Bulsara

Objectives: Socially constructed disablement has marginalised young people in families where a parent has younger onset dementia (YOD). This has contributed to inadequate societal support for their complex situation. Impacts on such young people include significant involvement with mental health services for themselves. In this paper we explored the young people’s lived experiences in these families and the influencing factors to enable these young people to be included and supported within their community.

Methods: In this qualitative research study the social model of disability was used as the theoretical framework in conducting a thematic analysis of interviews with 12 ...


Breast And Prostate Cancer Survivor Responses To Group Exercise And Supportive Group Psychotherapy, Eric Martin, Caroline Bulsara, Claudio Battaglini, Beth Hands, Fiona L. Naumann May 2016

Breast And Prostate Cancer Survivor Responses To Group Exercise And Supportive Group Psychotherapy, Eric Martin, Caroline Bulsara, Claudio Battaglini, Beth Hands, Fiona L. Naumann

Caroline Bulsara

This study qualitatively examined an 8 week group exercise and counseling intervention for breast and prostate cancer survivors. Groups exercised 3 days per week, 50 minutes per session, performing moderate intensity aerobic and resistance training. Groups also underwent 90 minute supportive group psychotherapy sessions once per week. Survivors discussed their experiences in focus groups post intervention. Transcripts were analyzed using interpretative phenomenological analysis. Survivors described how exercise facilitated counseling by creating mutual aid and trust, and counseling helped participants with self-identity, sexuality, and returning to normalcy. When possible, counselors and fitness professionals should create partnerships to optimally support cancer survivors.


The Phenc Project Final Report: Interactive Video Analysis To Develop Learning And Assessment Of University Students’ Practical And Communication Skills, Beth P. Hands, Helen Parker, Anne Coffey, Karen Clark-Burg, Annie Das, Pam Gerrard, Chris Hackett, Suzanne Jenkins, Jean Macnish, Donna Miller, Paul Rycroft, Christina Van Staden, Kate Gilomen, Brad Flynn May 2016

The Phenc Project Final Report: Interactive Video Analysis To Develop Learning And Assessment Of University Students’ Practical And Communication Skills, Beth P. Hands, Helen Parker, Anne Coffey, Karen Clark-Burg, Annie Das, Pam Gerrard, Chris Hackett, Suzanne Jenkins, Jean Macnish, Donna Miller, Paul Rycroft, Christina Van Staden, Kate Gilomen, Brad Flynn

Jean MacNish

The objective and authentic assessment of practical skills in tertiary settings is a challenge. The purpose of this project was to evaluate the usefulness of a video analysis software program as a teaching and/or assessment tool for practical and professional skills in five different undergraduate programs. Barnett’s (1989) model of ‘reflective thought through action’ highlights the importance of reflective observation of an event in order to plan and implement positive behavioural changes. When the practice activities are directly linked with expected skill outcomes then learning is meaningful and reflective practitioners are developed. Further, most Generation Y students better ...


Taxonomics – Next Generation Taxonomists, Ana Sofia Reboleira Apr 2016

Taxonomics – Next Generation Taxonomists, Ana Sofia Reboleira

Ana Sofia P.S. Reboleira

“Taxonomics” is proposed as a short name for the discipline of discovering, recognising, describing and classifying biological entities.


Ror-Γ Drives Androgen Receptor Expression And Represents A Therapeutic Target In Castration-Resistant Prostate Cancer, Jujian Wang, June X. Zou, Xiaoqian Xue, Demin Cai, Yan Zhang, Zguhuab Duan, Qiuping Xiang, Joy C. Yang, Maggie C. Louie, Alexander D Borowsky, Allen C. Gao, Christopher P. Evans, Kit S. Lam, Jianzhen Xu, Hsing-Jien Kung, Ronald M. Evans, Yong Xu, Hong-Wu Chen Apr 2016

Ror-Γ Drives Androgen Receptor Expression And Represents A Therapeutic Target In Castration-Resistant Prostate Cancer, Jujian Wang, June X. Zou, Xiaoqian Xue, Demin Cai, Yan Zhang, Zguhuab Duan, Qiuping Xiang, Joy C. Yang, Maggie C. Louie, Alexander D Borowsky, Allen C. Gao, Christopher P. Evans, Kit S. Lam, Jianzhen Xu, Hsing-Jien Kung, Ronald M. Evans, Yong Xu, Hong-Wu Chen

Maggie Louie

The androgen receptor (AR) is overexpressed and hyperactivated in human castration-resistant prostate cancer (CRPC). However, the determinants of AR overexpression in CRPC are poorly defined. Here we show that retinoic acid receptor-related orphan receptor γ (ROR-γ) is overexpressed and amplified in metastatic CRPC tumors, and that ROR-γ drives AR expression in the tumors. ROR-γ recruits nuclear receptor coactivator 1 and 3 (NCOA1 and NCOA3, also known as SRC-1 and SRC-3) to an AR-ROR response element (RORE) to stimulate AR gene transcription. ROR-γ antagonists suppress the expression of both AR and its variant AR-V7 in prostate cancer (PCa) cell lines and ...


On Person-Centred Therapy, Ebinepre A. Cocodia Apr 2016

On Person-Centred Therapy, Ebinepre A. Cocodia

Ebinepre Cocodia

No abstract provided.


Reflective Frameworks And The Assessment Of Ethical Issues, Ebinepre A. Cocodia Apr 2016

Reflective Frameworks And The Assessment Of Ethical Issues, Ebinepre A. Cocodia

Ebinepre Cocodia

No abstract provided.