Restoring Polyhyrdoxybutyrate (Phb) Depolymerase Expression In A Bald Mutant Of Streptomyces Sp. Sfb5a, 2021 Bridgewater College
Restoring Polyhyrdoxybutyrate (Phb) Depolymerase Expression In A Bald Mutant Of Streptomyces Sp. Sfb5a, Youmna Moawad
Polyhydroxybutyrate (PHB) is a biodegradable, plastic-like polymer produced by some bacteria and degraded by others. The filamentous bacterium Streptomyces sp. SFB5A degrades PHB for growth using an extracellular PHB depolymerase, encoded by the phaZ gene. A morphological mutant of this bacterium, bld4, cannot form aerial filaments and cannot produce PHB depolymerase, despite having the phaZ gene. The inability to synthesize PHB depolymerase may be due to a mutation in a gene encoding one of its transcriptional regulators. A gene (lrp) coding for a potential transcriptional regulator is located 2,700 base pairs upstream from phaZ. Our goal was to clone ...
Rare And Low Frequency Genomic Variants Impacting Neuronal Functions Modify The Dup7q11.23 Phenotype, 2021 University of Toronto
Rare And Low Frequency Genomic Variants Impacting Neuronal Functions Modify The Dup7q11.23 Phenotype, Farah Qaiser, Yue Yin, Carolyn B. Mervis, Colleen A. Morris, Bonita P. Klein-Tasman, Elaine Tam, Lucy R. Osborne, Ryan K.C. Yuen
School of Medicine Faculty Publications
© 2021, The Author(s). Background: 7q11.23 duplication (Dup7) is one of the most frequent recurrent copy number variants (CNVs) in individuals with autism spectrum disorder (ASD), but based on gold-standard assessments, only 19% of Dup7 carriers have ASD, suggesting that additional genetic factors are necessary to manifest the ASD phenotype. To assess the contribution of additional genetic variants to the Dup7 phenotype, we conducted whole-genome sequencing analysis of 20 Dup7 carriers: nine with ASD (Dup7-ASD) and 11 without ASD (Dup7-non-ASD). Results: We identified three rare variants of potential clinical relevance for ASD: a 1q21.1 microdeletion (Dup7-non-ASD) and two ...
Understanding Students’ Global Interdependence In Science Instruction, 2021 Texas Tech University
Understanding Students’ Global Interdependence In Science Instruction, Walter S. Smith
Journal of Global Education and Research
Multiple American educational organizations such as the National Education Association, Association for Supervision and Curriculum Development, and the Council of Chief State School Officers have advocated for globalizing the K-12 curriculum. The National Science Teaching Association (NSTA) in a position statement on international education and the Next Generation Science Standards have produced goals and standards for internationalizing the science curriculum by addressing topics such as climate change, environment, and disease that cross borders. In contrast to those pronouncements on the curriculum, this article views global science education through an instructional lens that focuses on a students’ global interdependence in science ...
Environmental Rnai Pathways In The Two-Spotted Spider Mite, 2021 The University of Arizona
Environmental Rnai Pathways In The Two-Spotted Spider Mite, Mosharrof Mondal, Jacob Peter, Obrie Scarbrough, Alex Flynt
© 2020, The Author(s).
Background:RNA interference (RNAi) regulates gene expression in most multicellular organisms through binding of small RNA effectors to target transcripts. Exploiting this process is a popular strategy for genetic manipulation and has applications that includes arthropod pest control. RNAi technologies are dependent on delivery method with the most convenient likely being feeding, which is effective in some animals while others are insensitive. The two-spotted spider mite, Tetranychus urticae, is prime candidate for developing RNAi approaches due to frequent occurrence of conventional pesticide resistance. Using a sequencing-based approach, the fate of ingested RNAs was explored to identify ...
Predicting The Spatiotemporal Exposure Of Aquatic Species To Intrusions Of Fire Retardant In Streams With Limited Data, Chris R. Rehmann, P. Ryan Jackson, Holly J. Puglis
Civil, Construction and Environmental Engineering Publications
Because fire retardant can enter streams and harm aquatic species including endangered fish, agencies such as the U.S. Forest Service (USFS) must estimate the downstream extent of toxic effects every time fire retardant enters streams (denoted as an “intrusion”). A challenge in estimating the length of stream affected by the intrusion and the exposure time of species in the affected reach is the lack of data typically available on the stream's geometry and flow characteristics. Previously, the USFS estimated the affected reach length assuming instantaneous mixing of the retardant over the reach; however, this approach neglects key river ...
Stream Sediment Monitoring In Morehead Kentucky, 2021 Morehead State University
Stream Sediment Monitoring In Morehead Kentucky, Kristopher Krolikowski, Jen O'Keefe
2021 Celebration of Student Scholarship - Oral Presentations
Sediment loads in stream systems play a big role in how overall runoff behaves. Streams naturally transport sediment at various ratios depending on precipitation. When vegetation is removed in upstream environments, sediment loads on streams increase. Sudden increases of sediments in streams can cause many issues, such as flooding or stream re-direction. In Morehead, KY, large-scale logging operations on the ridge above Knapp Avenue and on the ridge above the north end of Allen Avenue have caused increased sediment load in a small stream called Oxley Branch. This stream flows south parallel to Knapp Avenue and along the eastern property ...
Human Kit+ Myeloid Cells Facilitate Visceral Metastasis By Melanoma., 2021 The Jackson Laboratory
Human Kit+ Myeloid Cells Facilitate Visceral Metastasis By Melanoma., Chun I. Yu, Jan Martinek, Te-Chia Wu, Kyung In Kim, Joshy George, Elaheh Ahmadzadeh, Richard S. Maser, Florentina Marches, Patrick Metang, Pierre Authie, Vanessa K P Oliveira, Victor G Wang, Jeffrey Chuang, Paul Robson, Jacques Banchereau, Karolina Palucka
Faculty Research 2021
Metastasis of melanoma significantly worsens prognosis; thus, therapeutic interventions that prevent metastasis could improve patient outcomes. Here, we show using humanized mice that colonization of distant visceral organs with melanoma is dependent upon a human CD33+CD11b+CD117+ progenitor cell subset comprising <4% of the human CD45+ leukocytes. Metastatic tumor-infiltrating CD33+ cells from patients and humanized (h)NSG-SGM3 mice showed converging transcriptional profiles. Single-cell RNA-seq analysis identified a gene signature of a KIT/CD117-expressing CD33+ subset that correlated with decreased overall survival in a TCGA melanoma cohort. Thus, human CD33+CD11b+CD117+ myeloid cells represent a novel candidate biomarker as well as a therapeutic target for metastatic melanoma.
Meeting Minutes, June 4th, 2021, 2021 Eastern Washington University
Meeting Minutes, June 4th, 2021, National Smokejumper Association Board Of Directors
National Smokejumper Association Meeting Minutes
Agenda: Call to order, review and approval of Agenda; Consent Agenda; Information Items; Investment Report; Budget; Request for NSA Endorsement of USFS Covid 19 Preparedness Letter from National Wildfire Institute to Secretary Perdue; Trails Report; Scholarship Report; Keep the Flame Legacy Jump List; Q & A on Written Report; Identifying and Recruiting New Board Members; Information and Odds and Ends; In Attendance via Zoom;
Waste To Phosphorus: A Transdisciplinary Solution To P Recovery From Wastewater Based On The Triz Approach, 2021 Wroclaw University of Environmental and Life Sciences
Waste To Phosphorus: A Transdisciplinary Solution To P Recovery From Wastewater Based On The Triz Approach, Anna Jama-Rodzeńska, Andrzej Białowiec, Jacek A. Koziel, Józef Sowiński
Agricultural and Biosystems Engineering Publications
Phosphorus (P) is a limited yet essential resource. P cannot be replaced, but it can be recovered from waste. We proposed the TRIZ approach (Teoria reszenija izobretatielskich zadacz - Rus., Theory of Inventive Problem Solving - Eng.) to identify a feasible solution. We aimed at minimizing the environmental impact and, by eliminating contradictions, proposed viable technical solutions. P recovery can be more sustainable based on circular economy and 4Rs (reduction, recovery, reuse, and recycling). The TRIZ approach identified sewage sludge (SS) as waste with a large potential for P recovery (up to 90%). Successful selection and application of SS management and P ...
The Ripple Effect: ‘Training The Trainer’ On The Impact Of Body Image Through A Dance/Movement Therapy Technique, Marina Gearhart
Expressive Therapies Capstone Theses
This thesis describes the capstone project which was a psychoeducation and experiential workshop for fitness trainers. The workshop investigated the impact trainers’ work may have on the body image of their clients through a dance/movement therapy (DMT) technique. The aim was to enhance the trainers’ sensitivity around body image issues of their clients and to create a space for vulnerable movement conversation that may in turn lead to better mental health advocacy for their clients within the scope of ethical practice. The participants of the workshop were six white female identifying trainers ranging from 24 to 35 years of ...
Exploring The Usefulness Of Mindfulness Practices Through Zoom Meetings In Reducing Anxiety And/Or Stress Of Adults With Learning And Developmental Disabilities During The Covid-19 Pandemic, 2021 Lesley University
Exploring The Usefulness Of Mindfulness Practices Through Zoom Meetings In Reducing Anxiety And/Or Stress Of Adults With Learning And Developmental Disabilities During The Covid-19 Pandemic, Thatiane Abra
Expressive Therapies Capstone Theses
This thesis explored which mindfulness meditations or mindfulness practices could be beneficial in the online sessions, the extent to which adults with learning and developmental disabilities could learn the practices remotely, and how stress and anxiety disorders could be reduced through online mindfulness interventions. Furthermore, it was also necessary to address the online sessions as the only mental health treatment possibility during the Coronavirus pandemic for the clients observed and the benefits and limitations of these interventions. Additionally, the importance of structure and routine for this population, the multi-modal structure of the interventions, and how attunement plays an important role ...
The Impact Affective Teaching Has On High School Physical Education Student's Life Skills, 2021 The College at Brockport: State University of New York
The Impact Affective Teaching Has On High School Physical Education Student's Life Skills, Shane Derose
Kinesiology, Sport Studies, and Physical Education Synthesis Projects
As high school students graduate and move on towards their careers and lives that lay ahead of them, they most likely will implement what they have learned from school into their regular every day scenarios. What impacts these students can positively affect their life style choices, especially when it comes to physical education. Specifically, the affective domain provides students with many relevant learning points that they can use for the remainder of their lives. As physical education instructors, while it is crucial to focus on psychomotor and cognitive aspects of the content that is delivered, utilizing the affective domain ...
Designing A Reactive Warhead To Bind And Inhibit Pseudomonas Aeruginosa’S Periplasmic Protein, Inhibitor Of Vertebrate Lysozyme, 2021 Kennesaw State University
Designing A Reactive Warhead To Bind And Inhibit Pseudomonas Aeruginosa’S Periplasmic Protein, Inhibitor Of Vertebrate Lysozyme, Leah Greinke
Master of Science in Chemical Sciences Theses
Pseudomonas aeruginosa is a Gram-negative bacterium commonly found throughout the environment. It is a significant cause of disease and mortality in immunodeficient patients such as those suffering from cystic fibrosis (CF). Due to the emerging antibiotic resistance of P. aeruginosa, it is becoming increasingly more challenging to treat an infection by traditional means. Further complicating treatment, P. aeruginosa secretes a protein known as Inhibitor of Vertebrate Lysozyme (PaIVY) that binds to and inhibits C-type lysozyme, thus preventing the degradation of the bacterium. A reactive chemical warhead was synthesized from a rhenium(I) tricarbonyl derivative inorder to bind to and irreversibly ...
Ecology Of Estuarine Birds: Differences In Desensitization Of Year-Round And Transient Species, 2021 Coastal Carolina University
Ecology Of Estuarine Birds: Differences In Desensitization Of Year-Round And Transient Species, Sarah Thomas
Huntington Beach State Park in Murrells Inlet, SC, USA is considered a “hotspot” for birds, according to the eBird database (eBird, 2020). The park is also visited by approximately 300,000 people, annually (Hobdy, 2019). The causeway at the park is an area of high human and wildlife activity. Here, the differences in desensitization, or lack thereof, of year-round and transient bird species to the stimuli of vehicles and humans were determined. Multiple surveys of the birds at Huntington Beach State Park were conducted from 22nd September, 2019 to 13th June, 2020. Desensitization was quantified by use of a range ...
Detection Of Neonicotinoids In Northern Leopard Frog (Rana Pipiens) Brains, 2021 University of South Dakota
Detection Of Neonicotinoids In Northern Leopard Frog (Rana Pipiens) Brains, Peyton G. Keller
Neonicotinoids are widespread and commonly used to fight agricultural pests. Unfortunately, these neurotoxic insecticides commonly reach nearby wetlands due to tile drainage systems and agricultural runoff. Non-target organisms, such as amphibians, use wetlands as habitat and are likely exposed to elevated neonicotinoid levels. We collected Northern leopard frogs and water samples from control and tile wetlands to compare imidacloprid brain concentrations and subsequent changes in brain morphology. Additionally, a lab-based experiment was conducted to further analyze the ability of imidacloprid and its metabolite, imidacloprid-olefin, to cross the blood-brain barrier. Tile wetlands had higher aquatic imidacloprid concentrations. Subsequently, amphibians collected from ...
Catabolism Of Bcaas And Application, 2021 University of South Dakota
Catabolism Of Bcaas And Application, Skylar Arellano-Myers
Over the past 150 years, amino acids have been found and researched in the nutrition biochemistry field. Over these years, three have been identified; leucine, valine and isoleucine; to make up the branch chain amino acids (BCAAs) that play a crucial role on the anaerobic and aerobic energy systems. The metabolism of BCAAs is a highly complicated process that is regulated by the activation of the alpha-keto acids dehydrogenase complex (BCKDH complex) to complete the catabolic reaction. With exercise, it has been found to increase the metabolism of BCAAs by increasing the BCKDH complex, which will decrease the fatigue and ...
Evaluation Of Chili Pepper (Capsicum Annuum), Tiger Nuts (Cyperus Esculentus), And Turmeric (Curcuma Longa) As Sources Of Antioxidant Compounds For The Potential Of Antiaging-Like Activity, 2021 Jacksonville State University
Evaluation Of Chili Pepper (Capsicum Annuum), Tiger Nuts (Cyperus Esculentus), And Turmeric (Curcuma Longa) As Sources Of Antioxidant Compounds For The Potential Of Antiaging-Like Activity, Chelsea Toyer
Aging is a natural process that living organisms go through as they become older. In humans and other animals, aging is accompanied by several morphological changes, including the formation of wrinkles. Wrinkles are also caused by various environmental factors such as excessive exposure to sun or pollution, and continuous sleep deprivation. These environmental factors cause oxidation of skin cells and the degradation of subcutaneous fatty acids, leading to the formation of wrinkles. Skin care and wrinkle treatment products are part of a multimillion-dollar industry. Plant-based products are an alternative method for treating wrinkles and premature aging. Several antioxidants naturally found ...
Learn Grow Eat, 2021 Eastern Illinois University
Learn Grow Eat, Laura Dolezal, Arianne Kozeluh, Rachel Mannen
Health Fair: Tools & Lessons for K-12
No abstract provided.
Stage Of Estrous Cycle Influences Behavior After Social Stress, 2021 University of South Dakota
Stage Of Estrous Cycle Influences Behavior After Social Stress, Alexander Jonah Rodriguez
Since women are almost twice as likely to develop major depression compared to men, research into this and other potentially debilitating mood disorders using behavioral studies should involve females despite hormonal, physiological, and potential behavior changes due to reproductive cyclicity. Until recently, most studies exclusively used males, thus limiting results of previous research to half of the population. Using adult female C57BL/6N mice as model organisms, we exposed them to novel forms of social stress across five days which encompassed the four-day estrous cycle to determine if the stages of the estrous cycle had an impact on behavior when ...
In Vivo Digestibility, Intake And Nitrogen Balance Of Lambs Fed Different Barley Herbage: Sorghum Silage Diets, 2021 Universidad Nacional del Centro, Argentina
In Vivo Digestibility, Intake And Nitrogen Balance Of Lambs Fed Different Barley Herbage: Sorghum Silage Diets, M. Arzadun, S. Arias, J. Freddi, A. Piazza
International Grassland Congress Proceedings
No abstract provided.