Life Sciences Commons^™

Winter Rye (Secale Cereale L.) Management And Production Profitability In Kentucky, And Heritability Of Sensory Attributes, Elzbieta Szuleta

Theses and Dissertations--Plant and Soil Sciences

Winter rye (Secale cereale L.) is a small grain crop characterized by outstanding tolerance to drought, low temperatures, soil salinity and relatively low nutritional requirements. Rye is widely used as a cover crop, well known for its deep root system and outstanding nitrogen scavenging ability. This crop is widely used as a feed for livestock but also it is beneficial in human nutrition. Because of its versatile use, nutritional benefits and unique aroma and flavor, especially for bread and whiskey, there is an increasing interest in this crop in the United States. Unfortunately, rye grain production in the United …

Improving Baking Quality Of Soft Red Winter Wheat In Kentucky Through Breeding And Sulfur-Nitrogen Fertility Management, Maria Paula Castellari

Theses and Dissertations--Plant and Soil Sciences

Soft Red Winter (SRW) wheat is an important crop grown on about 400,000 acres in Kentucky as a pivotal cash crop in the 3-crops-in-2-years rotation of corn, wheat, and soybean. The humid environment of Kentucky generates a high yielding wheat crop, with low protein concentration and weak gluten strength. This wheat is mainly sold to millers and used by the industry for cakes, pastries, cookies, and crackers. Conversely, there is a rapidly growing interest in identifying other value-added markets for this wheat different to commodity commercialization, such as artisan-baked goods and craft-distilled beverages. Evaluating the quality characteristics of wheat grown …

Effects Of High-Speed Training On Messenger Rna Expression In Two-Year-Old Thoroughbred Racehorses, Mackenzie Grace Johnson

Theses and Dissertations--Veterinary Science

Accumulating high-speed exercise has been identified as a significant risk factor for catastrophic injuries in racing Thoroughbreds. Injuries, regardless of severity, are a main cause of withdrawal from the racing industry, raising animal welfare concerns and resulting in significant economic losses. While most of the current literature focuses on catastrophic injuries incurred during racing rather than training, the present study aims to help fill this gap as well as discuss the associated risk factors. The evaluation of messenger RNA (mRNA) expression changes provides an efficient and straightforward approach to identifying horses at risk for catastrophic injury. While alternative injury risk …

Molecular Genetic Studies Of Horses, Especially With Reference To Aggrecan And Dwarfism, John Edmund Eberth

Theses and Dissertations--Veterinary Science

This work consists of studies on dwarfism in Miniature horses and a study on breakdowns in Thoroughbreds in relation to gene aggrecan. A correction of the description and designation of D3 dwarf variant in aggrecan (ACAN) from the master’s thesis Chondrodysplasia-like dwarfism in the Miniature horse (2013). Commercial sequencing showed previous sequencing reads presented an artifact and not a single base deletion. Analysis showed a single base missense mutation in exon 8 identified as D3* was the actual cause. Multiple alleles of ACAN associated with chondrodysplastic dwarfism in Miniature horses by Eberth et al. (2018), corrected the …

Evolutionary History And Adaptation To Salinity In American Alligators, John Konvalina

Electronic Theses and Dissertations, 2020-

Stressful environments can commonly be found at the edge of a species range and may be a driver for adaption in suboptimal environments. Furthermore, the edge of a species' range can expand and contract over time, resulting in multiple independent invasions of the same stressful habitat. Elucidating population genetic structure and demographic history can aid in determining the which geologic factors impact range distributions and when climatic changes occurred driving genetic patterns observed in contemporary populations. Moreover, populations at the edge of the species range may adapt to the stressful environments that occur at the range edge and exhibit genetic …

Concrete Everywhere: A Project-Based Analysis Of The Unequal Distribution Of Warehouses In Fontana, Chanah Haigh

Scripps Senior Theses

Unjust legislation and zoning practices drive the excess of warehouses in Fontana, California whose impacts fall disproportionately on low income communities of color. This paper addresses the environmental and health impacts of warehouses, as well as the legislation and the use of zoning which bring them to Fontana. Original maps show the spread of warehouses over census tract level data depicting race, income, and environmental factors such as diesel particulate matter. State and local legislation is analyzed to determine its intended effect and how well it has been enforced. Research was conducted through a project based thesis done in conjunction …

Host Cell Responses Modulate Oncolytic Viral Treatment Of Neuroblastoma Cells, Kritika Kedarinath

Electronic Theses and Dissertations, 2020-

Neuroblastoma is an aggressive pediatric cancer that is poorly responsive to traditional cancer therapies. Oncolytic viral (OV) vectors such as Zika virus (ZIKV) and Parainfluenza virus type 5 (P/V virus) are promising neuroblastoma therapeutics, but the role of innate immune responses in the effectiveness of OV killing is not well understood. Previous studies showed the neuroblastoma cell line SK-N-AS (expressing low CD24) had low permissivity for ZIKV infection, and this restriction was relieved by ectopic CD24 expression (CD24-high cells). Compared to permissive CD24-high cells, the non-permissive CD24-low cells had elevated basal levels of IRF-1, NF?B and phosphorylated STAT1; these cells …

Potential Of Synthetic And Natural Compounds As Novel Histone Deacetylase Inhibitors For The Treatment Of Hematological Malignancies, Dilipkumar Pal, Khushboo Raj, Shyam Sundar Nandi, Surajit Sinha, Abhishek Mishra, Arijit Mondal, Ricardo Lagoa, Jack T. Burcher, Anupam Bishayee

Journal Articles: Cellular & Integrative Physiology

Histone deacetylases (HDACs) and histone acetyltransferases (HATs) are enzymes that remove or add acetyl groups to lysine residues of histones, respectively. Histone deacetylation causes DNA to more snugly encircle histones and decreases gene expression, whereas acetylation has the opposite effect. Through these small alterations in chemical structure, HATs and HDACs regulate DNA expression. Recent research indicates histone deacetylase inhibitors (HDACis) may be used to treat malignancies, including leukemia, B-cell lymphoma, virus-associated tumors, and multiple myeloma. These data suggest that HDACis may boost the production of immune-related molecules, resulting in the growth of CD8-positive T-cells and the recognition of nonreactive tumor …

Elevated Crp And Tnf-Α Levels Are Associated With Blunted Neural Oscillations Serving Fluid Intelligence, Sarah M. Dietz, Mikki Schantell, Rachel K. Spooner, Megan E. Sandal, Amirsalar Mansouri, Yasra Arif, Hannah J. Okelberry, Jason A. John, Ryan Glesinger, Pamela E. May, Elizabeth Heinrichs-Graham, Adam J. Case, Matthew C. Zimmerman, Tony W. Wilson

Journal Articles: Cellular & Integrative Physiology

INTRODUCTION: Inflammatory processes help protect the body from potential threats such as bacterial or viral invasions. However, when such inflammatory processes become chronically engaged, synaptic impairments and neuronal cell death may occur. In particular, persistently high levels of C-reactive protein (CRP) and tumor necrosis factor-alpha (TNF-α) have been linked to deficits in cognition and several psychiatric disorders. Higher-order cognitive processes such as fluid intelligence (Gf) are thought to be particularly vulnerable to persistent inflammation. Herein, we investigated the relationship between elevated CRP and TNF-α and the neural oscillatory dynamics serving Gf.

METHODS: Seventy adults between the ages of 20-66 years …

Commonalities And Differences In Carotid Body Dysfunction In Hypertension And Heart Failure, Igor S. A. Felippe, Rodrigo Del Río, Harold D. Schultz, Benedito H. Machado, Julian F R Paton

Journal Articles: Cellular & Integrative Physiology

Carotid body pathophysiology is associated with many cardiovascular-respiratory-metabolic diseases. This pathophysiology reflects both hyper-sensitivity and hyper-tonicity. From both animal models and human patients, evidence indicates that amelioration of this pathophysiological signalling improves disease states such as a lowering of blood pressure in hypertension, a reduction of breathing disturbances with improved cardiac function in heart failure (HF) and a re-balancing of autonomic activity with lowered sympathetic discharge. Given this, we have reviewed the mechanisms of carotid body hyper-sensitivity and hyper-tonicity across disease models asking whether there is uniqueness related to specific disease states. Our analysis indicates some commonalities and some potential …

Effects Of Forest Reclamation And Landscape Features On Avian Occupancy, Species Richness, And Abundance In Appalachia, Rebecca N. Davenport

Theses and Dissertations--Forestry and Natural Resources

The Forestry Reclamation Approach (FRA) is a recently developed coal mining reclamation method that emphasizes best management practices in forestry, such as the planting of native trees and shrubs. Although the FRA is expected to benefit wildlife, no studies have empirically examined the effects of the FRA on avian species. My study aimed to identify which reclamation approaches and/or landscape features promote breeding songbirds, particularly mature forest avian guilds and species of conservation need. I conducted point count surveys in the Appalachian Mountains of eastern West Virginia and assessed differences in avian occupancy, species richness, and species abundance between four …

Chemical Immobilization Of Helicopter-Captured Elk (Cervus Canadensis) And Survival Of Elk Calves In Southeastern Kentucky, Kathleen E. Williams

Theses and Dissertations--Forestry and Natural Resources

Safe and effective chemical immobilization is critical to minimize stress and risk of injury when capturing free-ranging, wild ungulates. Many traditionally favored high potency opioids have been phased out or become unavailable because of increased regulations, leading to the development of two pre-mixed combination drugs, butorphanol-azaperone-medetomidine (BAM) and nalbuphine-medetomidine-azaperone (NalMed-A). Both drugs have been used to chemically immobilize ungulates, but their efficacy has not been documented in elk captured and transported via helicopter. During 2020 – 2022, we chemically immobilized helicopter-captured female elk (Cervus canadensis) with a single IM-injection of BAM (n = 41) or NalMed-A (n = …

Applying Hermetic Storage And Multiple Abiotic-Stressed Germinations To Actuate Desirable Effects In Wheat Phytochemistry, Food, And Biological Functionalities, Bababode Kehinde

Theses and Dissertations--Biosystems and Agricultural Engineering

Wheat is one of the most widely consumed staple foods in the world, supplying both nutritional and caloric requirements for billions of people. The rising global population and increased food demand behooves all stakeholders across the wheat supply chain to significantly increase their output and efficiency. Beyond the target for increased farm yields, dietary, nutritional, and health requirements along with their continuous supply must be satisfied with improved storage and processing schemes. Post-harvest changes in wheat can result in a significant reduction in its quality. Such losses could be qualitative and/or quantitative, impacting its physical, physiological, organoleptic, and nutritional attributes. …

Profiling The Cell Wall Polysaccharides Of Hemp (Cannabis Sativa L.) Inflorescence And Hempseed And Exploring Their Application As Ruminant Feed Ingredients, Miranda Agbana

Theses and Dissertations--Animal and Food Sciences

Hemp (Cannabis sativa L.) is used in the United States as a source of CBD oil, fiber, and grain. While hempseeds are consumed whole or as dehulled hemp hearts, hempseed oil is also a desirable seed oil in human nutrition. The process of collecting hempseed oil leaves behind hempseed cakes that are abundant in protein and fiber. Feeding of hempseed cakes to animals, including ruminants, has only initially been explored. Extraction of CBD from hemp inflorescences generates organic waste that may find a second purpose as a fiber feed for cattle. Little is known about the exact fiber composition …

Personality Traits Of Dairy Calves And Their Relationship With Feeding Behavior, Activity, And Performance, Emily A. Michalski

Theses and Dissertations--Animal and Food Sciences

Detection of individual differences in personality traits of animals may prove beneficial for producers to help tailor management for individuals and to make selection decisions. Therefore, the objectives of this thesis were to

1) review and evaluate literature on relationships between individual cattle personality traits and feeding behavior, activity, and performance; and

2) evaluate and develop tests suitable for characterizing crossbred dairy-beef calves’ personalities and associations with behavioral patterns and performance.

The existing literature indicates that growth, intake, activity, and milk production measures from precision technologies have associations with cattle personality traits and behaviors identifiable through standardized tests. This indicates …

The Effects Of Exogenous Enzyme Supplementation On The Performance, Bone Quality, And Nutrient Utilization Of Broiler Chickens, Megan Bauer

Theses and Dissertations--Animal and Food Sciences

Two experiments were conducted to examine the effects of Allzyme® Spectrum supplementation to a reduced nutrient diet on the growth performance, energy and nutrient digestibility and utilization, bone quality, and jejunal digesta viscosity in broiler chickens. Each experiment used 300 one-day old Cobb by-product breeder chicks with 5 treatments, 10 replicates, and 6 birds per replicate cage in a randomized complete block design. The only differences between the two experiments were experiment 1 lasted for 21 days using a corn-soybean meal-based diet and experiment 2 lasted for 22 days using a wheat-soybean meal-based diet. The 5 treatments included a positive …

Table Of Contents, Annals Of Health Law And Life Sciences

Annals of Health Law and Life Sciences

No abstract provided.

Foreword, Micaela Enger

Annals of Health Law and Life Sciences

No abstract provided.

There's A Pill For That: The Supreme Court's Misguided Jurisprudence On Commercial Speech Under The First Amendment And Its Implications On Direct-To-Consumer Advertising Of Prescription Drugs, Benjamin Smith

Annals of Health Law and Life Sciences

No abstract provided.

Serving Two Masters: Conflicts Between Physician Employment Contracts And The Physician's Duty Of Care, Steven L. Hendler, M.D.

Annals of Health Law and Life Sciences

No abstract provided.

An Overlooked Argument For A Single-Payer Healthcare System: Eliminating Misalignment Among Payment Models, Jessica Mantel

Annals of Health Law and Life Sciences

No abstract provided.

Reflections On A Career Unplanned, Robert A. Glazer

Gulf and Caribbean Research

Sometimes life takes unexpected turns. I never planned to be a marine biologist; yet, after a long and unpredictable journey, that’s exactly where I found myself. After obtaining my B.S. in Fishery Biology from Colorado State University, I found myself meandering from job to job like a golden retriever following some vague scent. At first, I was hired by the Wyoming Game and Fish Department to work seasonally in Grand Teton National Park on the freshwater trout fisheries. Soon thereafter, I found myself cultivating oysters, clams, and other shellfish in California. The skills I developed there as an algologist led …

Lingnan Gardeners Newsletter (No. 65) = 彩園通訊 (第65期), Lingnan Gardeners, Centre For Cultural Research And Development, Lingnan University

Lingnan Gardeners Newsletter 嶺南彩園通訊

No abstract provided.

Telp Theory: Elucidating The Major Observations Of Rieger Et Al. 2021 In Mitochondria, James Weifu Lee

Chemistry & Biochemistry Faculty Publications

The transmembrane-electrostatically localized protons (TELP) theory may represent a complementary development to Mitchell's chemiosmotic theory. The combination of the two together can now excellently explain the energetics in mitochondria. Our calculated transmembrane-attractive force between an excess proton and an excess hydroxide explains how TELP may stay within a 1-nm thin layer at the liquid-membrane interface. Consequently, any pH sensor (sEcGFP) located at least 2–3 nm away from the membrane surface will not be able to see TELP. This feature as predicted from the TELP model was observed exactly in the experiment of Rieger et al., 2021. In contrast to their …

Expanding Our Grasp Of Two-Component Signaling In Clostridioides Difficile, Orlando Berumen Alvarez, Erin B. Purcell

Chemistry & Biochemistry Faculty Publications

The intestinal pathogen Clostridioides difficile encodes roughly 50 TCS, but very few have been characterized in terms of their activating signals or their regulatory roles. A. G. Pannullo, B. R. Zbylicki, and C. D. Ellermeier (J Bacteriol 205:e00164-23, 2023, https://doi.org/10.1128/jb.00164-23) have identified both for the novel C. difficile TCD DraRS. DraRS responds to antibiotics that target lipid-II molecules in the bacterial cell envelope, and regulates the production of a novel glycolipid necessary for bacitracin and daptomycin resistance in C. difficile.

Sound The (Smaller) Alarm: The Triphosphate Magic Spot Nucleotide Pgpp, Areej Malik, Megan A. Hept, Erin B. Purcell

Chemistry & Biochemistry Faculty Publications

It has recently become evident that the bacterial stringent response is regulated by a triphosphate alarmone (pGpp) as well as the canonical tetra- and pentaphosphate alarmones ppGpp and pppGpp [together, (p)ppGpp]. Often dismissed in the past as an artifact or degradation product, pGpp has been confirmed as a deliberate endpoint of multiple synthetic pathways utilizing GMP, (p)ppGpp, or GDP/GTP as precursors. Some early studies concluded that pGpp functionally mimics (p)ppGpp and that its biological role is to make alarmone metabolism less dependent on the guanine energy charge of the cell by allowing GMP-dependent synthesis to continue when GDP/GTP has been …

Enhancing The Conformational Stability Of The Cl-Par-4 Tumor Suppressor Via Site-Directed Mutagenesis, Samjhana Pandey, Krishna K. Raut, Andrea M. Clark, Antoine Baudin, Lamya Djemri, David S. Libich, Komala Ponniah, Steven M. Pascal

Chemistry & Biochemistry Faculty Publications

Intrinsically disordered proteins play important roles in cell signaling, and dysregulation of these proteins is associated with several diseases. Prostate apoptosis response-4 (Par-4), an approximately 40 kilodalton proapoptotic tumor suppressor, is a predominantly intrinsically disordered protein whose downregulation has been observed in various cancers. The caspase-cleaved fragment of Par-4 (cl-Par-4) is active and plays a role in tumor suppression by inhibiting cell survival pathways. Here, we employed site-directed mutagenesis to create a cl-Par-4 point mutant (D313K). The expressed and purified D313K protein was characterized using biophysical techniques, and the results were compared to that of the wild-type (WT). We have …

An Analysis Of Water Quality At Orange County, California Beaches, Naomi Meurice

Pomona Senior Theses

Beaches in Southern California are highly recreated by residents and visitors, making beaches socially and economically important. Public health departments in coastal communities are in charge of measuring water quality and ensuring it is safe for users. Research in the past has indicated that beach water quality gets worse after storms, with bacteria levels jumping on the day of a storm and staying high for up to five days. Studies have shown these spikes in bacteria to be associated with storm runoff, with beaches closer to runoff discharge locations experiencing more impact. However, prior research has not considered the period …

Effects Of Almond Hull Inclusion On Growth Performance Of Limit-Fed Growing Cattle, Z. M. Duncan, Z. L. Debord, M. G. Pflughoeft, K. J. Suhr, W. R. Hollenbeck, F. K. Brazle, E. C. Titgemeyer, K. C. Olson, D. A. Blasi

Kansas Agricultural Experiment Station Research Reports

Objective:The objective of this experiment was to evaluate the effects of almond hull inclusion and almond hull processing on growth performance of limit-fed growing beef cattle during a 56-day growing period.

Study Description:A total of 364 steers were assigned to one of four diets. The control diet contained (dry matter basis) 39.5% dry-rolled corn, 7.5% supplement, 40% wet-corn gluten feed, and 13% prairie hay. Almond hulls replaced prairie hay or prairie hay and dry-rolled corn and were fed at 13 and 26% of the diet, respectively. A subset of almond hulls was processed using a grinder mixer with …

Macrolichen Inventory Of The Horse Mountain Botanical Area, Six Rivers National Forest, California, Usa, Sarah Norvell Conway

Cal Poly Humboldt theses and projects

Macrolichen diversity and community composition was determined for an area of high botanical interest in the Coast Ranges of Northern California – the Horse Mountain Botanical Area (HMBA) in Six Rivers National Forest. The Coast Ranges have been suggested to have high epiphytic macrolichen diversity, yet detailed site-specific macrolichen surveys are lacking for the area. Here we present comprehensive data on macrolichens of the HMBA integrated with environmental metadata at the landscape level. Twenty 0.4 ha sampling plots were positioned across the varying habitats of the HMBA and macrolichens were intensively sampled from all substrata. Out of 888 total collections, …