Physical Sciences and Mathematics Commons^™

Environment Scan Of Generative Ai Infrastructure For Clinical And Translational Science, Hua Xu, Jiang Bian, Chunhua Weng, Yifan Peng, Betina Idnay, Zihan Xu, William G. Adams, Mohammad Adibuzzaman, Nicholas R. Anderson, Neil Bahroos, Douglas S. Bell, Cody Bumgardner, Thomas Campion, Mario Castro, James J. Cimino, I. Glenn Cohen, David Dorr, Peter L. Elkin, Jungwei W. Fan, Todd Ferris, David J. Foran, David Hanauer, Mike Hogarth, Kun Huang, Jayashree Kalpathy-Cramer, Manoj Kandpal, Niranjan S. Karnik, Avnish Katoch, Albert M. Lai, Christophe G. Lambert, Lang Li, Christopher Lindsell, Jinze Liu, Zhiyong Lu, Yuan Luo, Peter Mcgarvey, Eneida A. Mendonca, Parsa Mirhaji, Shawn Murphy, John D. Osborne, Ioannis C. Paschalidis, Paul A. Harris, Fred Prior, Nicholas J. Shaheen, Nawar Shara, Ida Sim, Umberto Tachinardi, Lemuel R. Waitman, Rosalind J. Wright, Adrian H. Zai, Kai Zheng, Sandra Soo-Jin Lee, Bradley A. Malin, Karthik Natarajan, Nicholson Price, Rui Zhang, Yiye Zhang

Articles

This study reports a comprehensive environmental scan of the generative AI (GenAI) infrastructure in the national network for clinical and translational science across 36 institutions supported by the CTSA Program led by the National Center for Advancing Translational Sciences (NCATS) of the National Institutes of Health (NIH) at the United States. Key findings indicate a diverse range of institutional strategies, with most organizations in the experimental phase of GenAI deployment. The results underscore the need for a more coordinated approach to GenAI governance, emphasizing collaboration among senior leaders, clinicians, information technology staff, and researchers. Our analysis reveals that 53% of …

Physicists As Environmental Experts, Rachel Rothschild

Articles

The question of which scientists are qualified to provide expert guidance in legal controversies is a perennial debate among scholars, judges, and lawyers. Scientists who participate in legal disputes can achieve enormous power and influence, not only over the case at hand but over long-term developments in legal doctrine. While these issues arise in many areas of the law, environmental litigation has been a particularly active site of contestations over epistemic authority. Courts have frequently relied on scientists to understand whether the government is justified in regulating pollution and who may be liable for environmental and public health harms.

This …

Clinicians In The Loop Of Medical Ai, W. Nicholson Price Ii

Articles

As medical AI begins to mature as a health-care tool, the task of governance grows increasingly important. Ensuring that medical AI works, works where it’s used, and works for the patient in the moment is a challenging, multifaceted task. Some of this governance can be centralized—in review by FDA or by national accreditation labs, for instance. Some must be local, performed by the hospital or health system about to use the product in their own, unique environment. But a large amount of governance is left to the individual provider in the room, the human in the loop who presumably knows …

Do Specialized Medical Llms Demand A Radically New Approach Under The Eu's Medical Device Regulation, Hannah Louise Smith, W. Nicholson Price Ii

Articles

We examine the arguments made by Onitiu and colleagues concerning the need to adopt a “backward-walking logic” to manage the risks arising from the use of Large Language Models (LLMs) adapted for a medical purpose. We examine what lessons can be learned from existing multi-use technologies and applied to specialized LLMs, notwithstanding their novelty, and explore the appropriate respective roles of device providers and regulators within the ecosystem of technological oversight.

An Analytical Model Of Motion Artifacts In A Measured Arterial Pulse Signal—Part I: Accelerometers And Ppg Sensors, Md Mahfuzur Rahman, Subodh Toraskar, Mamun Hasan, Zhili Hao

Mechanical & Aerospace Engineering Faculty Publications

This paper, the first of two parts, presents an analytical model of motion artifacts (MAs) in measured pulse signals by accelerometers and photoplethysmography (PPG) sensors. As the transmission path from the true pulse signal in an artery to the sensor output (measured pulse signal), the tissue-contact-sensor (TCS) stack is modeled as a 1DOF (degree-of-freedom) system. MAs cause baseline drift of the mass and simultaneously time-varying system parameters (TVSPs) of the TCS stack. With arterial wall displacement and pulsatile pressure serving separately as the true pulse signal, an analytical model is developed to mathematically relate baseline drift and TVSP to a …

Motion Artifacts Removal From Measured Arterial Pulse Signals At Rest: A Generalized Sdof-Model-Based Time-Frequency Method, Zhili Hao

Mechanical & Aerospace Engineering Faculty Publications

Motion artifacts (MA) are a key factor affecting the accuracy of a measured arterial pulse signal at rest. This paper presents a generalized time–frequency method for MA removal that is built upon a single-degree-of-freedom (SDOF) model of MA, where MA is manifested as time-varying system parameters (TVSPs) of the SDOF system for the tissue–contact-sensor (TCS) stack between an artery and a sensor. This model distinguishes the effects of MA and respiration on the instant parameters of harmonics in a measured pulse signal. Accordingly, a generalized SDOF-model-based time–frequency (SDOF-TF) method is developed to obtain the instant parameters of each harmonic in …

Optimal Control And Structurally-Informed Gradient Optimization Of A Custom 4-Dof Rigid-Body, Brock Marcinczyk, Logan E. Beaver

Mechanical & Aerospace Engineering Faculty Publications

This work develops a control-centric framework for a custom 4-DOF rigid-body manipulator by coupling a reduced-order Pontryagin’s Maximum Principle (PMP) controller with a physics-informed Gradient Descent stage. The reduced PMP model provides a closed-form optimal control law for the joint accelerations, while the Gradient Descent module determines the corresponding time horizons by minimizing a cost functional built directly from the full Rigid-Body Dynamics. Structural-mechanics reaction analysis is used only to initialize feasible joint velocities—most critically the azimuthal component—ensuring that the optimizer begins in a physically admissible region. The resulting kinematic trajectories and dynamically consistent time horizons are then supplied to …

Outgassing Measurements Of Bare And Magnetite-Coated Low-Carbon Steel Vacuum Chambers, Aiman H. Al-Allaq, Md Abdullah Mamun, Matt Poelker, Abdelmageed Elmustafa

Mechanical & Aerospace Engineering Faculty Publications

The outgassing properties of bare and magnetite-coated AISI 1020 low-carbon steel vacuum chambers were evaluated to establish material selection criteria for extreme high vacuum applications, namely, to explore the possibility of using these materials to build next-generation spin-polarized photoelectron guns. Water outgassing measurements using the throughput method revealed that the magnetite-coated chamber exhibited five times lower outgassing at room temperature prior to baking, but this advantage disappears after 80 °C baking. Hydrogen outgassing measurements demonstrated significant differences after intensive heat treatment: the bare low-carbon steel vacuum chamber achieved a specific outgassing rate of 9.6 × 10⁻¹⁶ Torr L s …

From Cyclones To Cybersecurity: A Call For Convergence In Risk And Crisis Communications Research, Ann Marie Reinhold, Ross J. Gore, Barry Ezell, Clemente I. Izurieta, Elizabeth A. Shanahan

VMASC Publications

Effective risk and crisis communication can improve health and safety and reduce harmful effects of hazards and disasters. A robust body of literature investigates mechanisms for improving risk and crisis communication. While effective risk and crisis communication strategies are equally desired across different hazard types (e.g., natural hazards, cyber security), the extent to which risk and crisis communication experts utilize the “lessons learned” from scientific domains outside their own is suspect. Therefore, we hypothesized that risk and crisis communication research is siloed according to academic disciplines at the detriment to the advancement of the field of risk communications research writ …

Applying Transfer Learning For Street-Scale Nuisance Flood Forecasting In Coastal-Urban Cities, Binata Roy, Jonathan L. Goodall, Diana Mcspadden, Chetan Kumar, Steven Goldenberg, Yidi Wang, Malachi Schram

VMASC Publications

An important challenge with Machine Learning (ML) is its transferability; i.e., whether a ML model trained on one set of data can be applied to a second set of data without requiring full re-training of the model. Transfer Learning (TL) addresses this challenge by transferring knowledge learned in the source domain (the data it was trained on) to the target domain (a second set of data that is statistically different but related, which the model was not trained on). This study investigates the use of TL for street-scale nuisance flood forecasting by exploring whether a ML model trained for one …

Ai-Generated Messaging For Life Events Using Structured Prompts: A Comparative Study Of Gpt With Human Experts And Machine Learning, Christopher Lynch, Erik Jensen, Ross Gore, Virginia Zamponi, Kevin O'Brien, Brandon Feldhaus, Katherine Smith, Joseph Martínez, Madison H. Munro, Timur E. Ozkose, Tugce B. Gundogdu, Ann Marie Reinhold, Hamdi Kavak, Barry Ezell

VMASC Publications

Large Language Models (LLMs) play an increasingly integrated and pivotal role in generating diverse types of texts, such as social media messages, emails, narratives, and technical reports, among other textual communication forms. As AI-generated messaging filters into human communication, a systematic exploration of their effectiveness for mimicking human-like communication of life events is needed. In this study, we employ a zero-shot structured narrative prompt to generate 24,000 life event messages for birth, death, hiring, and firing events using OpenAI's GPT-4. From this dataset, we manually classify 2880 messages and evaluate their validity in conveying these life events through the form …

Age Of Information-Based Optimal Scheduling With Energy Cost Trade-Off For Smart Warehouse: A Deep Reinforcement Learning-Based Approach, Sandip Roy, Abhishek Bisht, Ashok Kumar Das, Sachin Shetty

VMASC Publications

Recent advances in the integration of high-speed mobile networks and real-time IoT devices have facilitated in building of smart warehouses, where a set of beacons and Internet of Things (IoT) devices (or source nodes) can monitor the status of various physical processes in a time-critical way. In real-time status monitoring systems, like smart warehouses, quantifying the freshness of the Internet of Things (IoT) data based on the age of information (AoI) metrics becomes quite crucial. As source nodes are battery-constrained, a balanced trade-off between AoI minimization and preservation of source node battery energy is essential. In this paper, in a …

Faithful Narratives From Complex Conceptual Models: Should Modelers Or Large Language Models Simplify Causal Maps, Tyler J. Gandee, Philippe J. Giabbanelli

VMASC Publications

(1) Background: Comprehensive conceptual models can result in complex artifacts, consisting of many concepts that interact through multiple mechanisms. This complexity can be acceptable and even expected when generating rich models, for instance to support ensuing analyses that find central concepts or decompose models into parts that can be managed by different actors. However, complexity can become a barrier when the conceptual model is used directly by individuals. A ‘transparent’ model can support learning among stakeholders (e.g., in group model building) and it can motivate the adoption of specific interventions (i.e., using a model as evidence base). Although advances in …

Sting: A Stealthy Backdoor Attack On Gnn-Based Malicious Domain Detection Via Dns Perturbations, Muhammad Anan, Mahmoud Nazzal, Abdallah Khreishah, Issa Khalil, Nhathai Phan, Ahmad Sawalmeh

Computer Science Faculty Publications

Detecting malicious Internet domains is essential for safeguarding against various online threats. The current approach to detecting malicious domains (MDD) employs a graph neural network (GNN) method, which uses DNS logs to construct heterogeneous graphs for determining the maliciousness of unknown domains. Despite its success, this method is vulnerable to data poisoning attacks where an adversary can manipulate specific graph nodes to implant a backdoor into the model during training. To showcase the vulnerability, we propose a stealthy trigger injection attack on node features and graph structure in MDD, dubbed (STING). The attacker carefully manipulates selected features and edges of …

Normalizing Images In Various Weather And Lighting Conditions Using Colorpix2pix Generative Adversarial Network, Sanjida Tasnim, Ashif Mahmud Mostafa, Azmain Morshed, Namreen Shaiyaz, Shakib Mahmud Dipto, Saad Aloteibi, Mohammad Ali Moni, Md. Golam Rabiul Alam, Md. Ashraful Alam

Computer Science Faculty Publications

Autonomous vehicles (AVs) are widely regarded as the future of transportation due to their tremendous benefits and user comfort. However, the AVs have been struggling with very crucial challenges, such as achieving reliable accuracy in object detection as well as faster computation required for quick decision-making. In recent years, perception systems in driverless cars have been significantly enhanced, mainly due to advances in deep-learning-based object detection systems. However, these perception systems are still heavily affected by environmental variables, such as changes in illumination, refractive interference, and adverse weather conditions, which may compromise their reliability and safety. This research proposes an …

Coming Back Differently: An Exploratory Case Study Of Near Death Experiences Of Webpages, Lesley Frew, Michael L. Nelson, Michele Weigle

Computer Science Faculty Publications

In this case study, we use web archives to analyze 8,824 webpages that were taken offline and subsequently put back online, thus experiencing a “near death experience.” We enumerate the stages of a webpage’s near death experience, including the change from a successful HTTP status code to non-successful and back, the intermediate stage with markers such as an under construction banner, and an analysis of how the pages came back differently.

5g-Practical Byzantine Fault Tolerance: An Improved Pbft Consensus Algorithm For The 5g Network, Xin Liu, Xing Fan, Baoning Niu, Xianrong Zheng

Information Technology & Decision Sciences Faculty Publications

The consensus algorithm is the core technology of blockchain systems to maintain data consistency, and its performance directly affects the efficiency and security of the whole system. Practical Byzantine Fault Tolerance (PBFT) plays a crucial role in blockchain consensus algorithms by providing a robust mechanism to achieve fault-tolerant and deterministic consensus in distributed networks. With the development of 5G network technology, its features of high bandwidth, low latency, and high reliability provide a new approach for consensus algorithm optimization. To take advantage of the features of the 5G network, this paper proposes 5G-PBFT, which is an improved practical Byzantine fault-tolerant …

Towards Dynamic Learner State: Orchestrating Ai Agents And Workplace Performance Via The Model Context Protocol, Mohan Yang, Nolan Lovett, Belle Li, Zhen Hou

Educational Leadership & Workforce Development Faculty Publications

Current learning and development approaches often struggle to capture dynamic individual capabilities, particularly the skills they acquire informally every day on the job. This dynamic creates a significant gap between what traditional models think people know and their actual performance, leading to an incomplete and often outdated understanding of how ready the workforce truly is, which can hinder organizational adaptability in rapidly evolving environments. This paper proposes a novel dynamic learner-state ecosystem—an AI-driven solution designed to bridge this gap. Our approach leverages specialized AI agents, orchestrated via the Model Context Protocol (MCP), to continuously track and evolve an individual’s multi-dimensional …

Feel Bad To Discard A Fashion Product: How Ai Designers Influence Individuals' Sustainable Consumption, Ha Kyung Lee, Dooyoung Choi

Educational Leadership & Workforce Development Faculty Publications

This study explores how AI technology in fashion design influences consumers' sustainable consumption behaviors, focusing on emotional attachment to products. By comparing AI-generated and human-designed fashion items, the study examines how designer type impacts negative emotions about discarding products, mediated by emotional attachment. Results from two experimental studies reveal that designer type significantly affects negative emotions toward discarding human-designed items, but emotional attachment was not influenced by designer type in the first study. This lack of difference may be due to personal characteristics that moderate the effect. The second study found that individuals who perceive AI as human-like form stronger …

Exploring The Impact Of Value Co-Creation Through Ai-Driven Chatbbots On Customer Repeat Purchases, Dooyoung Choi, Jaeha Lee

Educational Leadership & Workforce Development Faculty Publications

Drawing on the Stimulus-Organism-Response (S-O-R) framework, this study explores how perceived value co-creation during chatbot interactions influences customer repeat purchase intentions through cognitive, emotional, and social responses to chatbots. A survey of 220 participants revealed that perceived value co-creation significantly affected repeat purchase intentions, with cognitive evaluations, emotional reactions, and social value serving as key mediators. However, the direct effect of value co-creation on purchase intentions was not significant. The findings suggest that while value co-creation enhances consumer engagement, repeat purchases occur only when consumers experience positive cognitive, emotional, and social outcomes. Therefore, it is crucial for retailers to incorporate …

Examining Teaching Competencies And Challenges While Integrating Artificial Intelligence In Higher Education, Xinyue Ren, Min Lun Wu

STEMPS Faculty Publications

The rapid development of artificial intelligence (AI) technologies has demonstrated their affordances and limitations in revolutionizing pedagogical strategies in higher education. Given the lack of guidelines, policies, and resources to assist instructors in efficiently and ethically integrating AI into teaching and learning practices, this systematic review aimed to investigate AI integration competencies and challenges in higher education from the intelligent Technological Pedagogical Content Knowledge (TPACK) perspective. We first applied the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) to identify 23 studies published between 2019 and 2023 that met the inclusion and exclusion criteria. After conducting open coding and …

Advancing Pedagogical And Instructional Design Through Artificial Intelligence (Ai) In Education And Training Contexts, Mohan Yang, Jewoong Moon, Tian Luo, Jinhee Kim

STEMPS Faculty Publications

[Introduction] "The only way to discover the limits of the possible is to go beyond them into the impossible." - Arthur C. Clarke

It is our pleasure and honor as guest editors for this special issue of the Journal of Applied Instructional Design (JAID) to present "Advancing Pedagogical and Instructional Design through Artificial Intelligence (AI) in Education and Training Contexts." This issue arrives at a truly timely moment, as the rapid and continuous development of generative artificial intelligence (GenAI) is fundamentally reshaping the traditional paradigms of teaching, learning, and training.

Analysing Nontraditional Students' Chatgpt Interaction, Engagement, Self-Efficacy And Performance: A Mixed-Methods Approach, Mohan Yang, Shiyan Jiang, Belle Li, Kristin Herman, Tian Luo, Shanan Chappell Moots, Nolan Lovett

STEMPS Faculty Publications

Generative artificial intelligence brings opportunities and unique challenges to nontraditional higher education students, stemming, in part, from the experience of the digital divide. Providing access and practice is critical to bridge this divide and equip students with needed digital competencies. This mixed-methods study investigated how nontraditional higher education students interact with ChatGPT in multiple courses and examined relationships between ChatGPT interactions, engagement, self-efficacy and performance. Data were collected from 73 undergraduate and graduate students through chat logs, course reflections and artefacts, surveys and interviews. ChatGPT interactions were analysed using four metrics: prompt number, depth of knowledge (DoK), prompt relevance and …

Seasonality Of Submarine Groundwater Discharge Pathways In A Coastal Lagoon Revealed By Radium Isotopes: The Importance Of Porewater Exchange In Summer, Júlia Rodriguez-Puig, Valentí Rodellas, Marc Diego-Feliu, Andres Alcolea, Joaquín Jiménez-Martínez, Irene Alorda-Montiel, Aaron Alorda-Kleinglass, Francisco Pereira, Marisol Manzano, Javier Gilabert, Jordi Garcia-Orellana

OES Faculty Publications

Chemical fluxes supplied by Submarine Groundwater Discharge (SGD) can be relevant for coastal ecosystems, particularly lagoonal environments. An appropriate understanding of SGD requires constraining the magnitude and composition of its different pathways. However, the evaluation of SGD pathways is often limited to independent estimations, lacking a comprehensive understanding of the groundwater processes at play. In this study, we combined a Ra mass balance and hydrogeological modeling to simultaneously estimate different SGD pathways flowing into a coastal lagoon. We assessed water fluxes derived from fresh SGD, long-scale recirculation of seawater, and porewater exchange (PEX) into Mar Menor, a highly anthropized coastal …

Atmospheric Chemistry Experiment (Ace) Winds, Matthew Wyatt, Peter F. Bernath, Chris Boone, Léo Lavy, Ryan Johnson

Chemistry & Biochemistry Faculty Publications

The Atmospheric Chemistry Experiment Fourier Transform Spectrometer (ACE-FTS) uses limb geometry to measure transmittance spectra of Earth's atmosphere by solar occultation. Line-of-sight wind speeds can be derived via Doppler shifts of molecular lines in infrared spectra. The wind look direction angles relative to geodetic North are derived from geometry. We validate the new ACE version 5.3 (v.5.3) line-of-sight winds with MIGHTI and meteor radar vector wind observations and find a ±15 m/s sunrise/sunset shift above 80 km. We also compare line-of-sight winds from ACE-FTS v.5.2 and v.5.3 with vector winds from the MERRA-2, HWM14, and WACCM-X models. A ±15 m/s …

Evaluating The Chemical Reactivity Of Wildfire-Derived Dissolved Organic Molecules: Glutathione Binding Through Kendrick Mass Defect Analysis, Hannah M. Hamontree, Patrick G. Hatcher

Chemistry & Biochemistry Faculty Publications

The emerging risks to organisms of pyrogenic-derived dissolved organic matter (PyDOM) from forest fires are of concern due to its toxic and mutagenic potential (e.g., pro-oxidative responses in fauna through the depletion of glutathione, a nitrogen- and sulfur-containing tripeptide found in cells). This study simulates this phenomenon in a laboratory setting by identifying bonding between reduced l-glutathione and organic molecules in leachates from environmentally weathered biomass samples (charred and uncharred) using Kendrick Mass Defect (KMD) analysis from formula lists obtained from negative-mode electrospray ionization-Fourier transform-ion cyclotron resonance-mass spectrometry ((-)ESI-FT-ICR-MS). These formula lists reveal a 10-fold increase in nitrogen- and sulfur-containing …

The Importance Of Atomic Charges For Predicting Site-Selective Ir-, Ru-, And Rh-Catalyzed C-H Borylations, Shannon M. Stephens, Kyle M. Lambert

Chemistry & Biochemistry Faculty Publications

A supervised machine learning model has been developed that allows for the prediction of site selectivity in late-stage C-H borylations. Model development was accomplished using literature data for the site-selective (≥95%) C-H borylation of 189 unique arene, heteroarene, and aliphatic substrates that feature a total of 971 possible sp² or sp³ C-H borylation sites. The reported experimental data was supplemented with additional chemoinformatic descriptors, computed atomic charges at the C-H borylation sites, and data from parameterization of catalytically active tris-boryl complexes resulting from the combination of seven different Ir-, Ru-, and Rh-based precatalysts with eight different ligands. Of the over …

Challenges Associated With Pfas Detection Method In Africa, Abdullahi Tunde Aborode, Ridwan Olamilekan Adesola, Ibrahim Idris, Waheed Sakariyau Adio, Segun Olapade, Gladys Oluwafisayo, Israel Ayobami Onifade, Sodiq Fakorede, Taiwo Bakare-Abidola, Jelil Olaoye, Adedeji Daniel Ogunyemi, Oluwaseun Adeolu Ogundijo, Olamilekan Gabriel Banwo, Adetolase Azizat Bakre, Peter Oladoye, Grace Adegoye, Noimat Abeni Jinadu

Chemistry & Biochemistry Faculty Publications

Per- and polyfluoroalkyl substances (PFAS) are a group of man-made chemicals that are widely present in many industries. Monitoring and analyzing PFAS in Africa is challenging due to the limited availability of mass spectrometry (MS), which is an essential technique for detecting PFAS. This review assesses the scope and impact of the shortage of mass spectrometry instruments in Africa, emphasizing the resulting limitations in monitoring environmental and public health threats. The review analyzes the existing PFAS monitoring, the accessibility of MS instruments, and the technical capabilities within the continent. This study suggests that fewer African countries have sufficient MS instruments, …

Nucleotide-Derived Bacterial Alarmones Attenuate The Induction Of Type-I Interferon Responses In A Murine Macrophage Reporter Cell Line, Ryan P. Kilduff, Erin B. Purcell, Lisa M. Shollenberger

Chemistry & Biochemistry Faculty Publications

The stringent response is a well-studied phenomenon in many bacterial systems and regulates resource-consuming activities such as transcription, translation, and replication. The stringent response is a well-conserved signaling framework, as are the nucleotide-derived signaling mediators, collectively referred to as (p)ppGpp or as alarmones. There is a wealth of research evaluating nucleotide-derived alarmone signaling in bacterial models, however, their potential to modulate innate immune signaling has not yet been evaluated. Several common pathogen-synthesized molecules, such as lipopolysaccharide (LPS) and cyclic-di-AMP (c-di-AMP), act as pathogen-associated molecular patterns (PAMPs), which are common patterns that alert the innate immune system of bacterial infection. The …

Green Chelation Strategy For Deashing Of Algal Biomass, Agyare Asante, George Daramola, Ryan W. Davis, Sandeep Kumar

Civil & Environmental Engineering Faculty Publications

This study investigated a green chelation strategy for deashing algal biomass using nitrilotriacetic acid (NTA) and deionized water (DI) to enhance its suitability for biofuel and bioproduct applications. Solid-state algal turf scrubber (SS ATS), green algal turf scrubber (ATS), and Scenedesmus were analyzed, with Scenedesmus selected for detailed evaluation due to its high ash removal efficiency. The objective was to optimize a purification process that minimizes ash and heavy metal content while preserving biochemical integrity. Algal biomass underwent sequential washing with DI, NTA, and NTA+DI under varying temperatures (90-130 °C). Analytical techniques including Fourier Transform Infrared (FTIR) spectroscopy, Inductively Coupled …