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Laboratory and Basic Science Research Commons

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328 full-text articles. Page 1 of 9.

Inhibition Of Breast Cancer Cell Proliferation By Diarylthiourea Analog, Sl1-18, Maryam Fallatah 2015 Dominican University of California

Inhibition Of Breast Cancer Cell Proliferation By Diarylthiourea Analog, Sl1-18, Maryam Fallatah

Scholarly and Creative Works Conference

Breast cancer is the most common malignancy that occurs in women in the US and has the second highest cancer-associated mortality rate. Depending on the expression of estrogen receptor α (ERα), breast cancer can be classified as ER positive or negative. Current drugs used to treat ER+ cancers include selective estrogen receptor modulators (SERMs) and selective estrogen receptor down-regulators (SERDs). Unfortunately, prolonged exposure to these drugs often leads to the development of acquired resistance. Consequently, there is a great need to develop alternative therapeutic options. Flexible heteroarotinoids (Flex-Hets) are groups of compounds that are derived from retinoids. Flex-Hets have been ...


Investigation Of Structure-Activity Relationships Of Antimalarial Drugs Through Novel Plasmodium Falciparum Chloroquine Resistance Resistance Transporter (Pfcrt), Bianca Sanchez Mora, Samuel Lemus, Danielle Nicole Flordeliza, Jillian Nunes, Alexis Valdovinos 2015 Dominican University of California

Investigation Of Structure-Activity Relationships Of Antimalarial Drugs Through Novel Plasmodium Falciparum Chloroquine Resistance Resistance Transporter (Pfcrt), Bianca Sanchez Mora, Samuel Lemus, Danielle Nicole Flordeliza, Jillian Nunes, Alexis Valdovinos

Scholarly and Creative Works Conference

Mutation in the P. falciparum resistance chloroquine transporter (PfCRT) protein causes chloroquine resistance and alters susceptibility to various antimalarial drugs. Mutant PfCRT transports chloroquine out of the parasite digestive vacuole; however, the molecular details of drug-receptor interaction are poorly understood. The antiviral drug amantadine has moderate potency against P. falciparum and acts to reduce the level of chloroquine resistance determined by the pfcrt haplotype. In accordance, chloroquine-resistant parasites show enhanced sensitivity to amantadine. We used continuous, step-wise amantadine selection on three chloroquine-resistant lines of the P. falciparum 106/1 strain. Each of these lines contains a unique pfcrt allele differing ...


Evaluation Of The Developmental Toxicity Of Lead In The Danio Rerio Body, Nicole M. Roy, Sarah DeWolf, Bruno Carneiro 2015 Sacred Heart University

Evaluation Of The Developmental Toxicity Of Lead In The Danio Rerio Body, Nicole M. Roy, Sarah Dewolf, Bruno Carneiro

Biology Faculty Publications

Lead has been utilized throughout history and is widely distributed and mobilized globally. Although lead in the environment has been somewhat mitigated, the nature of lead and its extensive uses in the past prohibit it from being completely absent from our environment and exposure to lead is still a public health concern. Most studies regarding lead toxicity have focused on the brain. However, little is found in the literature on the effects of lead in other tissues. Here, we utilize the zebrafish model system to investigate effects of lead exposure during early developmental time windows at 24, 48 and 72 ...


Estimates Of Persistent Inward Current In Human Motor Neurons During Postural Sway, Ryan Foley, Jayne M. Kalmar Dr. 2015 Wilfrid Laurier University

Estimates Of Persistent Inward Current In Human Motor Neurons During Postural Sway, Ryan Foley, Jayne M. Kalmar Dr.

Theses and Dissertations (Comprehensive)

Persistent inward current (PIC) is a membrane property critical for increasing gain of motor neuron output. In humans, most estimates of PIC are made from plantarflexor or dorsiflexor motor units with the participant in a seated position with the knee flexed. This seated and static posture neglects the task-dependent nature of the monoaminergic drive that modulates PIC activation. Seated estimates may drastically underestimate the amount of PIC that occurs in human motor neurons during functional movement. The current study estimated PIC using the conventional paired motor unit technique which uses the difference between reference unit firing frequency at test unit ...


Determining The Critical Window Of Influence Of Pcb Perinatally On Behavioral And Hormonal Development In Sprague-Dawley Rat Pups, Natalie Sommerville, Lee A. Meserve, Howard C. Cromwell 2014 Bowling Green State University - Main Campus

Determining The Critical Window Of Influence Of Pcb Perinatally On Behavioral And Hormonal Development In Sprague-Dawley Rat Pups, Natalie Sommerville, Lee A. Meserve, Howard C. Cromwell

Honors Projects

Polychlorinated biphenyls (PCB) had widespread use in the United States in commercial manufacturing in the United States until the late 1970s. Even though they were banned, measurable amounts can still be found in the environment and food sources. PCB has known effects on altering hormone development and behavior in the species Rattus norvegicus. To determine the most crucial developmental time of exposure to PCB in Sprague-Dawley rat pups, rat pups were exposed to PCB at differing weeks of either gestation period or the first postnatal week. Behavioral tests were performed for the different rat pups, as well as blood serum ...


Autoantibodies To The Ny-Eso-1 Tumor Antigen In Metastatic Melanoma: Sialylation Of The Fc Region Of Immunoglobulin G Induces Differential Expression Signatures Of Inflammatory Molecules During Dendritic Cell Differentiation And Maturation, Martin Oaks, Nathaniel Rein, John O. Richards, James Shaffer 2014 Histocompatibility and Transplant Research

Autoantibodies To The Ny-Eso-1 Tumor Antigen In Metastatic Melanoma: Sialylation Of The Fc Region Of Immunoglobulin G Induces Differential Expression Signatures Of Inflammatory Molecules During Dendritic Cell Differentiation And Maturation, Martin Oaks, Nathaniel Rein, John O. Richards, James Shaffer

Journal of Patient-Centered Research and Reviews

Purpose: We tested the hypothesis that different glycoforms of antibodies from patients with metastatic melanoma have different functional effects on human dendritic cell differentiation and maturation.

Methods: Antibodies to the cancer antigen NY-ESO-1 were affinity-purified from patients with melanoma and further fractionated into different glycoforms by lectin chromatography. Sialic acid-rich and sialic acid-poor fractions of these immunoglobulin G antibodies (IgG) were added to dendritic cell cultures during both differentiation and maturation, and the resulting cellular messenger RNA (mRNA) and culture supernatants were tested by microarray and enzyme-linked immunoassay for molecules related to inflammatory pathways.

Results: We identified unique mRNA and ...


Wentworth Point History Bulletin # 12, Randy Lackovic 2014 University of Maine - Main

Wentworth Point History Bulletin # 12, Randy Lackovic

Darling Marine Center Historical Documents

This history recounts the formation of the Darling Marine Center from 1963 - 1966.


Wentworth Point History Bulletin # 10, Randy Lackovic 2014 University of Maine - Main

Wentworth Point History Bulletin # 10, Randy Lackovic

Darling Marine Center Historical Documents

This is a history of the marine and freshwater sciences activity at the University of Maine from 1865 - 1965.


Wentworth Point History Bulletin # 11, Randy Lackovic 2014 University of Maine - Main

Wentworth Point History Bulletin # 11, Randy Lackovic

Darling Marine Center Historical Documents

This is picture album of the University of Maine Marine Biological Laboratory at Lamoine, Maine during the summer session in 1939.


Natural Egg Sac Clutch Size Of The Brown Widow Spider, Latrodectus Geometricus (Araneae: Theridiidae) In Southern California, Douglas W. R. Danielsen, Daniel E. Clarke, Stephan J. Valle, Amy A. Anselmo, Leonard S. Vincent, Richard S. Vetter 2014 Fullerton College

Natural Egg Sac Clutch Size Of The Brown Widow Spider, Latrodectus Geometricus (Araneae: Theridiidae) In Southern California, Douglas W. R. Danielsen, Daniel E. Clarke, Stephan J. Valle, Amy A. Anselmo, Leonard S. Vincent, Richard S. Vetter

Bulletin of the Southern California Academy of Sciences

Abstract. We investigated egg sac clutch size of the brown widow spider, Latrodectus geometricus C. L. Koch, in southern California from June through October 2012 by counting eggs from 214 field collected egg sacs. Clutch size was fairly constant over this five-month period averaging 129.0 ± 51.0 eggs per sac with the only significant monthly difference being more eggs in September than June. Determining the relatively constant egg production for this period contributed knowledge to another project involving application of pesticide to brown widow egg sacs in attempted control of this newly established invasive pest species.


Annotated Bibliography: Attitudes Toward Animal Research (1998-2013), Erich Yahner 2014 Humane Society Institute for Science and Policy

Annotated Bibliography: Attitudes Toward Animal Research (1998-2013), Erich Yahner

Humane Education Bibliographies

No abstract provided.


Pi3k- And Mtor-Dependent Mechanisms Of Lapatinib Resistance And Resulting Therapeutic Opportunities, Samuel Brady 2014 University of Texas Health Science Center at Houston

Pi3k- And Mtor-Dependent Mechanisms Of Lapatinib Resistance And Resulting Therapeutic Opportunities, Samuel Brady

UT GSBS Dissertations and Theses (Open Access)

Breast cancers with HER2 amplification represent 20-25% of breast cancer cases and are frequently responsive to the HER2 kinase inhibitor lapatinib, but generally for only short duration. We aimed to understand how breast cancers with HER2 amplification become resistant to lapatinib, in order to identify potential therapies that can overcome lapatinib resistance. To establish lapatinib resistance models we treated three HER2+ breast cancer cell lines with lapatinib for several months until they became lapatinib-resistant. We then compared lapatinib-sensitive (parental) cells with their lapatinib-resistant (LapR) counterparts to identify changes conferring lapatinib resistance. We found that activation of PI3K, specifically the p110α ...


Development Of Chimeric Type Iv Secretion Systems For Transfer Of Heterologous Substrates Across The Gram-Negative Cell Envelope, Trista M. Berry 2014 University of Texas Health Science Center at Houston

Development Of Chimeric Type Iv Secretion Systems For Transfer Of Heterologous Substrates Across The Gram-Negative Cell Envelope, Trista M. Berry

UT GSBS Dissertations and Theses (Open Access)

Many bacteria use Type IV Secretion Systems (T4SSs) to aid in pathogenesis by translocating virulence factors across the cell envelope and into eukaryotic cells. These systems are structurally and functionally diverse, but are often compared to the archetypal VirB/VirD4 T4SS of Agrobacterium tumefaciens. This system is composed of the VirD4 type IV coupling protein (T4CP) and 11 VirB subunits (VirB1-11) that assemble as the secretion channel and an extracellular pilus. The T4CP is an inner membrane ATPase that interacts with T4SS substrates and the secretion channel, and is thought to link substrates with the secretion channel and possibly energize ...


Energy Stress Causes Chaperones To Assemble Into Cytoplasmic Complexes, Kimberly J. Cope 2014 The University of Texas Graduate School of Biomedical Sciences at Houston

Energy Stress Causes Chaperones To Assemble Into Cytoplasmic Complexes, Kimberly J. Cope

UT GSBS Dissertations and Theses (Open Access)

The majority of proteins require molecular chaperones to assist their folding into tertiary and quaternary structures. Certain stresses can compromise the weak hydrophobic forces responsible for these structures and lead to protein unfolding, misfolding, and aggregation. Aggregates of proteins are hallmarks of devastating diseases such as Alzheimer’s, Parkinson’s, and Huntington’s diseases. Fortunately, bacteria, plants, and fungi have a potent disaggregase, named Hsp104 in Saccharomyces cerevisiae. Recently, heat-induced aggregates, termed Q-bodies, were found to contain three molecular chaperones: Hsp70, Hsp104, and Hsp42. Their coalescence from small puncta into larger inclusions required Hsp104. During glucose deprivation, a stress that ...


Reactive Oxygen Species And P21 Waf1/Cip1 Are Both Essential For, Alison Fitzgerald 2014 The University of Texas Graduate School of Biomedical Sciences at Houston

Reactive Oxygen Species And P21 Waf1/Cip1 Are Both Essential For, Alison Fitzgerald

UT GSBS Dissertations and Theses (Open Access)

Treatment of Head and Neck Squamous Cell Carcinoma, HNSCC, often requires multimodal therapy, including radiation therapy. The efficacy of radiotherapy in controlling locoregional recurrence, the most frequent cause of death from HNSCC, is critically important for patient survival. One potential biomarker to determine radioresistance is TP53, whose alterations are predictive of poor radiation response. The following work shows that the p53 transcriptional target, p21, is crucial in initiating and maintaining senescence in HNSCC, through its ability to regulate reactive oxygen species (ROS). With the use of a novel system to evaluate the impact of the TP53 missense mutations, we were ...


Physical Activity In Individuals With Down Syndrome, Chelsea B. Spurgeon 2014 University of Tennessee - Knoxville

Physical Activity In Individuals With Down Syndrome, Chelsea B. Spurgeon

University of Tennessee Honors Thesis Projects

No abstract provided.


A Comparison Of Fatigue During Cardiocerebral Resuscitation With Different Compression Rates Among Layperson And Professional Rescuers, Christopher Cassidy 2014 Cal Poly State University

A Comparison Of Fatigue During Cardiocerebral Resuscitation With Different Compression Rates Among Layperson And Professional Rescuers, Christopher Cassidy

Master's Theses and Project Reports

Quality chest compressions during Cardiopulmonary Resuscitation (CPR) are vital to maintaining adequate perfusion of oxygenated blood to the organs of the body to sustain life. Over the years, the compression rate recommended in Basic Life Support (BLS)/ Advanced Cardiac Life Support (ACLS) protocols for the best possible outcome has risen, and with that increase there are questions regarding rescuer fatigue and the effectiveness of compressions. Layperson and professional rescuers, answering to an emergency, both maintain continuous chest compressions until advanced life support arrives. Depending on the location, this arrival time would most likely be longer than the two minute standard ...


Characterization Of The Anti-Apoptotic Function Of The Lysine Demethylase Plant Homeodomain Finger Protein 8 (Phf8), Kimberly Muranko 2014 The University of Western Ontario

Characterization Of The Anti-Apoptotic Function Of The Lysine Demethylase Plant Homeodomain Finger Protein 8 (Phf8), Kimberly Muranko

University of Western Ontario - Electronic Thesis and Dissertation Repository

Apoptosis is an essential process in development and tissue maintenance. The tumor suppressor protein p53 initiates apoptosis through transactivation of pro-apoptotic genes when cellular stress is detected. This study identifies a regulatory role for the lysine demethylase, PHF8, in the p53-mediated apoptosis pathway. We initially suspected PHF8 of demethylating the adaptor protein Numb, however found this to be untrue. PHF8 has been found to have oncogenic properties including an anti-apoptotic effect, however how PHF8 negatively affects apoptosis has not been previously investigated. We found PHF8 inhibits translation of the pro-apoptotic genes TP53, BAX and CASP3. Chromatin immunoprecipitation revealed PHF8 binding ...


The Role Of Integrins In Support Of Pancreatic Function, Survival And Maturation, Matthew Riopel 2014 The University of Western Ontario

The Role Of Integrins In Support Of Pancreatic Function, Survival And Maturation, Matthew Riopel

University of Western Ontario - Electronic Thesis and Dissertation Repository

The pancreas is a glandular organ composed of endocrine and exocrine compartments. Integrins are cell adhesion molecules that connect cells to the extracellular matrix (ECM). Integrins modulate a variety of cellular effects, yet their mechanism of action in the developed pancreas is not well understood. Fibrin is a provisional ECM protein that contains ligands for integrin receptors. Fibrin is capable of supporting islet health, but it is unclear how fibrin exerts its effects. The objective of this thesis is to understand the role of integrin receptors on in vivo pancreatic cell function, survival, and proliferation. In addition, this thesis investigates ...


Evaluating Colostrum Quality With Niacin Supplementation In The Diet Of Dairy Cattle, Allison Marie Pike 2014 University of New Hampshire - Main Campus

Evaluating Colostrum Quality With Niacin Supplementation In The Diet Of Dairy Cattle, Allison Marie Pike

Student Research Projects

Dairy calves are born with a naive immune system, and must obtain passive immunity through ingestion of colostrum. Colostrum provides the newborn with antimicrobial proteins called immunoglobulins, such as immunoglobulin G (IgG), that protect the calf for the first few weeks of life. Calves that receive good quality colostrum (>50 g/L of IgG) are more likely to survive to adulthood. Niacin is a vasodilator that may increase blood flow to the mammary gland during production of colostrum in the mother. This experiment investigated the effect of niacin supplementation at a rate of 48 g/cow/day for 3 weeks ...


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