Efficient In Vitro Development Of Photoreceptors From Human Pluripotent Stem Cells, 2015 Dominican University of California
Efficient In Vitro Development Of Photoreceptors From Human Pluripotent Stem Cells, Joseph C. Reynolds
Master's Theses and Capstone Projects
Degeneration of the rod and cone photoreceptors in the human retina is among the most common causes of blindness. Replacing these damaged photoreceptors may help to restore vision. Repairing the damaged retina relies on the insertion of new, healthy cells. Embryonic stem (ES) cells and induced pluripotent stem (iPS) cells are two possible sources of photoreceptors to restore vision. Previous data shows that human ES cells and iPS cells can be differentiated into photoreceptors and transplanted into the eye to restore some vision. However, this process is inefficient, and costly. Here, we show a new method for inducing photoreceptor production ...
Inhibition Of Breast Cancer Cell Proliferation By Diarylthiourea Analog, Sl1-18, 2015 Dominican University of California
Inhibition Of Breast Cancer Cell Proliferation By Diarylthiourea Analog, Sl1-18, Maryam Fallatah
Scholarly and Creative Works Conference
Breast cancer is the most common malignancy that occurs in women in the US and has the second highest cancer-associated mortality rate. Depending on the expression of estrogen receptor α (ERα), breast cancer can be classified as ER positive or negative. Current drugs used to treat ER+ cancers include selective estrogen receptor modulators (SERMs) and selective estrogen receptor down-regulators (SERDs). Unfortunately, prolonged exposure to these drugs often leads to the development of acquired resistance. Consequently, there is a great need to develop alternative therapeutic options. Flexible heteroarotinoids (Flex-Hets) are groups of compounds that are derived from retinoids. Flex-Hets have been ...
Investigation Of Structure-Activity Relationships Of Antimalarial Drugs Through Novel Plasmodium Falciparum Chloroquine Resistance Resistance Transporter (Pfcrt), 2015 Dominican University of California
Investigation Of Structure-Activity Relationships Of Antimalarial Drugs Through Novel Plasmodium Falciparum Chloroquine Resistance Resistance Transporter (Pfcrt), Bianca Sanchez Mora, Samuel Lemus, Danielle Nicole Flordeliza, Jillian Nunes
Scholarly and Creative Works Conference
Mutation in the P. falciparum resistance chloroquine transporter (PfCRT) protein causes chloroquine resistance and alters susceptibility to various antimalarial drugs. Mutant PfCRT transports chloroquine out of the parasite digestive vacuole; however, the molecular details of drug-receptor interaction are poorly understood. The antiviral drug amantadine has moderate potency against P. falciparum and acts to reduce the level of chloroquine resistance determined by the pfcrt haplotype. In accordance, chloroquine-resistant parasites show enhanced sensitivity to amantadine. We used continuous, step-wise amantadine selection on three chloroquine-resistant lines of the P. falciparum 106/1 strain. Each of these lines contains a unique pfcrt allele differing ...
Resolution Of Telomere Fragility In Mammalian Celss, 2015 Washington University in St Louis
Resolution Of Telomere Fragility In Mammalian Celss, Mai R. Nguyen
Undergraduate Research Symposium Posters & Abstracts
Genomic stability is vital for the survival of cells and organisms. Telomeres, the nucleoprotein complexes capping the ends of linear chromosomes, prevent loss of genetic information and play an important role in the regulation of cell division. Due to their repetitive sequence and ability to form secondary structures, telomeres are especially sensitive to mutations or deletions of DNA replication and repair proteins. Telomere fragility, a phenotype in which multiple telomere signals or aberrant signals are observed on metaphase chromosomes stained by fluorescent in situ hybridization, is induced when cells experience replication stress. Although telomere fragility has been observed in many ...
Mcnamara 2014 Mmb1075 - Low Magnification Confocal Microscopy Of Tumor Angiogenesis, 2015 M.D. Anderson Cancer Center
Mcnamara 2014 Mmb1075 - Low Magnification Confocal Microscopy Of Tumor Angiogenesis, George Mcnamara
McNamara 2014 MMB1075 - Low Magnification Confocal Microscopy of Tumor Angiogenesis
Book chapter from Paddock 2014 Confocal Microscopy
Book is online at http://www.springer.com/us/book/9781588293510
Investigating Notch Signaling And Sequential Segmentation In The Fairy Shrimp, Thamnocephalus Platyurus, Sara Izzat Khalil
Senior Theses and Projects
Segmentation is a key feature of arthropod diversity and evolution. In the standard model for arthropod development, Drosophila melanogaster, segments develop simultaneously by a progressive subdivision of the embryo. By contrast, most arthropods add segments sequentially from a posterior region called the growth zone and in a manner similar to vertebrates.
Recent work, mainly focused on insects, suggests that Notch signaling might play a role in arthropods that segment sequentially. These studies document a potential regulatory similarity between sequentially segmenting arthropods and vertebrates. In vertebrates, somite formation involves a molecular oscillator that functions as a pacemaker, driving periodic expression of ...
Evaluation Of The Developmental Toxicity Of Lead In The Danio Rerio Body, 2015 Sacred Heart University
Evaluation Of The Developmental Toxicity Of Lead In The Danio Rerio Body, Nicole M. Roy, Sarah Dewolf, Bruno Carneiro
Biology Faculty Publications
Lead has been utilized throughout history and is widely distributed and mobilized globally. Although lead in the environment has been somewhat mitigated, the nature of lead and its extensive uses in the past prohibit it from being completely absent from our environment and exposure to lead is still a public health concern. Most studies regarding lead toxicity have focused on the brain. However, little is found in the literature on the effects of lead in other tissues. Here, we utilize the zebrafish model system to investigate effects of lead exposure during early developmental time windows at 24, 48 and 72 ...
Estimates Of Persistent Inward Current In Human Motor Neurons During Postural Sway, 2015 Wilfrid Laurier University
Estimates Of Persistent Inward Current In Human Motor Neurons During Postural Sway, Ryan Foley, Jayne M. Kalmar Dr.
Theses and Dissertations (Comprehensive)
Persistent inward current (PIC) is a membrane property critical for increasing gain of motor neuron output. In humans, most estimates of PIC are made from plantarflexor or dorsiflexor motor units with the participant in a seated position with the knee flexed. This seated and static posture neglects the task-dependent nature of the monoaminergic drive that modulates PIC activation. Seated estimates may drastically underestimate the amount of PIC that occurs in human motor neurons during functional movement. The current study estimated PIC using the conventional paired motor unit technique which uses the difference between reference unit firing frequency at test unit ...
Regulation Of Pancreatic Β-Cell Function By The Renin-Angiotensin System In Type 2 Diabetes, 2015 University of Kentucky
Regulation Of Pancreatic Β-Cell Function By The Renin-Angiotensin System In Type 2 Diabetes, Robin C. Shoemaker
Theses and Dissertations--Pharmacology and Nutritional Sciences
Diet-induced obesity promotes type 2 diabetes (T2D). Drugs that inhibit the renin-angiotensin system (RAS) have been demonstrated in clinical trials to decrease the onset of T2D. Previously, we demonstrated that mice made obese from chronic consumption of a high-fat (HF) diet have marked elevations in systemic concentrations of angiotensin II (AngII). Pancreatic islets have been reported to possess components of the renin-angiotensin system (RAS), including angiotensin type 1a receptors (AT1aR), the primary receptor for AngII, and angiotensin converting-enzyme 2 (ACE2), which negatively regulates the RAS by catabolizing AngII to angiotensin-(1-7) (Ang-(1-7)). These two opposing proteins have been implicated ...
Potencial Impacts Of Ecosystem Degradation On The Reproductive Biology And Behavior Of Malawian Cichlids, 2015 University of Colorado, Boulder
Potencial Impacts Of Ecosystem Degradation On The Reproductive Biology And Behavior Of Malawian Cichlids, Michael Abel
Undergraduate Honors Theses
The Rift Valley is a tropical region of eastern Africa characterized by its complex geological makeup consisting of tectonic plates that have driven the formation of some of the largest lakes in the world being Lake Malawi, Victoria, and Tanganyika. In turn, the formation of these amazing freshwater ecosystems has enabled the evolution, and radiation of thousands of species of fish from the family Cichlidae, which constitutes the most diverse group of vertebrates on the globe. However, because these ecosystems are also in regions with a high prevalence of anthropogenic activities they are threatened by biodiversity loss. In particular, Lake ...
Mouse Polyomavirus T Antigens: Directors Of Cell Cycle Signaling, 2015 University of Colorado, Boulder
Mouse Polyomavirus T Antigens: Directors Of Cell Cycle Signaling, Catherine Nicholas
Undergraduate Honors Theses
Polyomaviruses (PyV) efficiently replicate by disrupting host cell signaling pathways. Disruption of the cell cycle is implicated in nearly all tumor formation. Studies of cellular transformation by primate PyV, SV40, and mouse polyomavirus (MPyV) have led to numerous findings concerning tumor suppressor proteins and cell cycle regulation pathways (Das D. and Imperiale 2009, Dahl et. al. 2005). Expression of PyV T Antigens (TAg) modifies signaling pathways and cell cycle checkpoints to the virus’ advantage. Expected modifications include inhibiting checkpoint proteins between G1 and S phases as well as promoting kinases with downstream signaling effects that result in progression to S ...
Expectations For Methodology And Translation Of Animal Research: A Survey Of Health Care Workers, 2015 University of Alberta
Expectations For Methodology And Translation Of Animal Research: A Survey Of Health Care Workers, Ari Joffe, Meredith Bara, Natalie Anton, Nathan Nobis
Animal Research, Testing, and Experimentation
Background: Health care workers (HCW) often perform, promote, and advocate use of public funds for animal research (AR); therefore, an awareness of the empirical costs and benefits of animal research is an important issue for HCW. We aim to determine what health-care-workers consider should be acceptable standards of AR methodology and translation rate to humans.
Methods: After development and validation, an e-mail survey was sent to all pediatricians and pediatric intensive care unit nurses and respiratory-therapists (RTs) affiliated with a Canadian University. We presented questions about demographics, methodology of AR, and expectations from AR. Responses of pediatricians and nurses/RTs ...
Determining The Critical Window Of Influence Of Pcb Perinatally On Behavioral And Hormonal Development In Sprague-Dawley Rat Pups, 2014 Bowling Green State University - Main Campus
Determining The Critical Window Of Influence Of Pcb Perinatally On Behavioral And Hormonal Development In Sprague-Dawley Rat Pups, Natalie Sommerville, Lee A. Meserve, Howard C. Cromwell
Polychlorinated biphenyls (PCB) had widespread use in the United States in commercial manufacturing in the United States until the late 1970s. Even though they were banned, measurable amounts can still be found in the environment and food sources. PCB has known effects on altering hormone development and behavior in the species Rattus norvegicus. To determine the most crucial developmental time of exposure to PCB in Sprague-Dawley rat pups, rat pups were exposed to PCB at differing weeks of either gestation period or the first postnatal week. Behavioral tests were performed for the different rat pups, as well as blood serum ...
Examining The Role Of Dax-1 In Regulation Of Cell Proliferation In Human Breast Cells, 2014 University of San Francisco
Examining The Role Of Dax-1 In Regulation Of Cell Proliferation In Human Breast Cells, Amy E. Scandurra
DAX-1 is a member of the Nuclear Hormone Receptor superfamily and acts as a transcriptional repressor. DAX-1 plays an important role in the development of adrenal and gonadal tissues. In addition to its role in normal cell development and differentiation, DAX-1 appears to have some influence on the progression of cancer. This work aims to examine the role of DAX-1 in regulation of proliferation in breast cancer. In our study, we have expressed DAX-1 in a DAX-1 deficient breast cancer cell line as well as knocked down DAX-1 expression in normal DAX-1 positive breast cells. Through these experiments, we were ...
Autoantibodies To The Ny-Eso-1 Tumor Antigen In Metastatic Melanoma: Sialylation Of The Fc Region Of Immunoglobulin G Induces Differential Expression Signatures Of Inflammatory Molecules During Dendritic Cell Differentiation And Maturation, 2014 Histocompatibility and Transplant Research
Autoantibodies To The Ny-Eso-1 Tumor Antigen In Metastatic Melanoma: Sialylation Of The Fc Region Of Immunoglobulin G Induces Differential Expression Signatures Of Inflammatory Molecules During Dendritic Cell Differentiation And Maturation, Martin Oaks, Nathaniel Rein, John O. Richards, James Shaffer
Journal of Patient-Centered Research and Reviews
Purpose: We tested the hypothesis that different glycoforms of antibodies from patients with metastatic melanoma have different functional effects on human dendritic cell differentiation and maturation.
Methods: Antibodies to the cancer antigen NY-ESO-1 were affinity-purified from patients with melanoma and further fractionated into different glycoforms by lectin chromatography. Sialic acid-rich and sialic acid-poor fractions of these immunoglobulin G antibodies (IgG) were added to dendritic cell cultures during both differentiation and maturation, and the resulting cellular messenger RNA (mRNA) and culture supernatants were tested by microarray and enzyme-linked immunoassay for molecules related to inflammatory pathways.
Results: We identified unique mRNA and ...
Wentworth Point History Bulletin # 12, 2014 University of Maine - Main
Wentworth Point History Bulletin # 12, Randy Lackovic
Darling Marine Center Historical Documents
This history recounts the formation of the Darling Marine Center from 1963 - 1966.
Wentworth Point History Bulletin # 10, 2014 University of Maine - Main
Wentworth Point History Bulletin # 10, Randy Lackovic
Darling Marine Center Historical Documents
This is a history of the marine and freshwater sciences activity at the University of Maine from 1865 - 1965.
Wentworth Point History Bulletin # 11, 2014 University of Maine - Main
Wentworth Point History Bulletin # 11, Randy Lackovic
Darling Marine Center Historical Documents
This is picture album of the University of Maine Marine Biological Laboratory at Lamoine, Maine during the summer session in 1939.
Natural Egg Sac Clutch Size Of The Brown Widow Spider, Latrodectus Geometricus (Araneae: Theridiidae) In Southern California, Douglas W. R. Danielsen, Daniel E. Clarke, Stephan J. Valle, Amy A. Anselmo, Leonard S. Vincent, Richard S. Vetter
Bulletin of the Southern California Academy of Sciences
Abstract. We investigated egg sac clutch size of the brown widow spider, Latrodectus geometricus C. L. Koch, in southern California from June through October 2012 by counting eggs from 214 field collected egg sacs. Clutch size was fairly constant over this five-month period averaging 129.0 ± 51.0 eggs per sac with the only significant monthly difference being more eggs in September than June. Determining the relatively constant egg production for this period contributed knowledge to another project involving application of pesticide to brown widow egg sacs in attempted control of this newly established invasive pest species.
Annotated Bibliography: Attitudes Toward Animal Research (1998-2013), 2014 Humane Society Institute for Science and Policy
Annotated Bibliography: Attitudes Toward Animal Research (1998-2013), Erich Yahner
Humane Education Bibliographies
No abstract provided.