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229 full-text articles. Page 1 of 7.

Natural Egg Sac Clutch Size Of The Brown Widow Spider, Latrodectus Geometricus (Araneae: Theridiidae) In Southern California, Douglas W. R. Danielsen, Daniel E. Clarke, Stephan J. Valle, Amy A. Anselmo, Leonard S. Vincent, Richard S. Vetter 2014 Occidental College

Natural Egg Sac Clutch Size Of The Brown Widow Spider, Latrodectus Geometricus (Araneae: Theridiidae) In Southern California, Douglas W. R. Danielsen, Daniel E. Clarke, Stephan J. Valle, Amy A. Anselmo, Leonard S. Vincent, Richard S. Vetter

Bulletin of the Southern California Academy of Sciences

Abstract. We investigated egg sac clutch size of the brown widow spider, Latrodectus geometricus C. L. Koch, in southern California from June through October 2012 by counting eggs from 214 field collected egg sacs. Clutch size was fairly constant over this five-month period averaging 129.0 ± 51.0 eggs per sac with the only significant monthly difference being more eggs in September than June. Determining the relatively constant egg production for this period contributed knowledge to another project involving application of pesticide to brown widow egg sacs in attempted control of this newly established invasive pest species.


Annotated Bibliography: Attitudes Toward Animal Research (1998-2013), Erich Yahner 2014 Humane Society Institute for Science and Policy

Annotated Bibliography: Attitudes Toward Animal Research (1998-2013), Erich Yahner

Humane Education

No abstract provided.


Physical Activity In Individuals With Down Syndrome, Chelsea B. Spurgeon 2014 University of Tennessee, Knoxville

Physical Activity In Individuals With Down Syndrome, Chelsea B. Spurgeon

University of Tennessee Honors Thesis Projects

No abstract provided.


Pi3k- And Mtor-Dependent Mechanisms Of Lapatinib Resistance And Resulting Therapeutic Opportunities, Samuel Brady 2014 Texas Medical Center Library

Pi3k- And Mtor-Dependent Mechanisms Of Lapatinib Resistance And Resulting Therapeutic Opportunities, Samuel Brady

UT GSBS Dissertations and Theses (Open Access)

Breast cancers with HER2 amplification represent 20-25% of breast cancer cases and are frequently responsive to the HER2 kinase inhibitor lapatinib, but generally for only short duration. We aimed to understand how breast cancers with HER2 amplification become resistant to lapatinib, in order to identify potential therapies that can overcome lapatinib resistance. To establish lapatinib resistance models we treated three HER2+ breast cancer cell lines with lapatinib for several months until they became lapatinib-resistant. We then compared lapatinib-sensitive (parental) cells with their lapatinib-resistant (LapR) counterparts to identify changes conferring lapatinib resistance. We found that activation of PI3K, specifically the p110α ...


The Role Of Integrins In Support Of Pancreatic Function, Survival And Maturation, Matthew Riopel 2014 Western University

The Role Of Integrins In Support Of Pancreatic Function, Survival And Maturation, Matthew Riopel

University of Western Ontario - Electronic Thesis and Dissertation Repository

The pancreas is a glandular organ composed of endocrine and exocrine compartments. Integrins are cell adhesion molecules that connect cells to the extracellular matrix (ECM). Integrins modulate a variety of cellular effects, yet their mechanism of action in the developed pancreas is not well understood. Fibrin is a provisional ECM protein that contains ligands for integrin receptors. Fibrin is capable of supporting islet health, but it is unclear how fibrin exerts its effects. The objective of this thesis is to understand the role of integrin receptors on in vivo pancreatic cell function, survival, and proliferation. In addition, this thesis investigates ...


Evaluating Colostrum Quality With Niacin Supplementation In The Diet Of Dairy Cattle, Allison Marie Pike 2014 University of New Hampshire

Evaluating Colostrum Quality With Niacin Supplementation In The Diet Of Dairy Cattle, Allison Marie Pike

Student Research Projects

Dairy calves are born with a naive immune system, and must obtain passive immunity through ingestion of colostrum. Colostrum provides the newborn with antimicrobial proteins called immunoglobulins, such as immunoglobulin G (IgG), that protect the calf for the first few weeks of life. Calves that receive good quality colostrum (>50 g/L of IgG) are more likely to survive to adulthood. Niacin is a vasodilator that may increase blood flow to the mammary gland during production of colostrum in the mother. This experiment investigated the effect of niacin supplementation at a rate of 48 g/cow/day for 3 weeks ...


A Longitudinal View Of Primate Life In Two American Laboratories, Jonathan Balcombe 2014 Humane Society Institute for Science and Policy

A Longitudinal View Of Primate Life In Two American Laboratories, Jonathan Balcombe

Animal Research, Testing, and Experimentation

If representative of other facilities, our findings uncover serious welfare concerns for the wellbeing of primates kept in American research facilities. These animals face regular or chronic sources of pain and distress including noxious experimental and non‐experimental events and illness and injury; and severe and prolonged social disruptions. Pain relief is meager by comparison to that normally provided to humans, despite legislative requirements to minimize pain and distress and assume similarity to humans in terms of ability to experience pain and distress. Living environments are usually confinement indoors to a metal cage, often alone, and often with a minimum ...


Gain-Of-Function Mouse Models To Investigate Biological Roles Of Prmt6, Alessandra Di Lorenzo 2014 Texas Medical Center Library

Gain-Of-Function Mouse Models To Investigate Biological Roles Of Prmt6, Alessandra Di Lorenzo

UT GSBS Dissertations and Theses (Open Access)

Gain-of-function Mouse Models to Investigate Biological Roles of PRMT6

Alessandra Di Lorenzo, Ph.D. Candidate

Mentor: Dr. Mark T. Bedford

Protein Arginine Methyltransferase 6 (PRMT6) is the histone tail writer that methylates the H3R2 (arginine 2 of histone H3) residue, which counteracts the activating H3K4me3 mark. PRMT6 has been shown to behave both as transcriptional co-repressor (i.e. trhrombospondin-1, p21, p53), and co-activator (nuclear receptors). The co-repressor function of PRMT6 is likely the result of H3K4me3 antagonism, while the mechanism by which PRMT6 exerts its co-activator function has yet to be elucidated. PRMT6 is over-expressed in several types of tumors ...


Investigations Into Aldefluor As A Novel Method For Identifying Leukemia In Soft-Shell Clams, Katherine F. Norwood 2014 University of New Hampshire

Investigations Into Aldefluor As A Novel Method For Identifying Leukemia In Soft-Shell Clams, Katherine F. Norwood

Student Research Projects

The clam species Mya arenaria is a common model organism in leukemia research. The current method for classifying the degree of cancer progression is by examining cell morphology with light microscopy. This approach is highly qualitative, which makes differentiation of pre-leukemic and semi-leukemic individuals difficult. One quantitative approach that may differentiate individuals is based on levels of aldehyde dehydrogenase (ALDH) expression. The enzyme assay ALDEFLUOR® can actively measure ALDH expression in viable cells, but the effectiveness of certain protocol conditions is dependent upon the cell type.


Exploration Of Freshwater Cyanobacteria, Mariya Campbell 2014 Georgia State University

Exploration Of Freshwater Cyanobacteria, Mariya Campbell

Georgia State Undergraduate Research Conference

No abstract provided.


Salt Marsh Values In A Changing World: Examining Sea Level Rise On Tidal Marshes With A Surface Elevation Table, Sarah Martina Tierney 2014 University of New Hampshire

Salt Marsh Values In A Changing World: Examining Sea Level Rise On Tidal Marshes With A Surface Elevation Table, Sarah Martina Tierney

Student Research Projects

Rising seas are threatening coastal communities and putting added pressures on the natural environment. Sea level rise rates are increasing on a global scale (from 1.7 to 3.2 mm/yr). Salt marshes are not only intertidal habitats acutely influenced by sea levels, but they also provide key ecosystem services such as: buffers against storm surges, habitat for wildlife, carbon dioxide storage, and pollutant filtration. In New England, salt marshes have built at a rate of 1 to 2 mm annually over the past 4,000 years, which has kept pace with sea level rise. However, we do not ...


Investigations Into The Use Of Aldefluor® As An Alternative Method To Classify Leukemia In The Soft-Shell Clam Mya Arenaria., Katherine F. Norwood 2014 University of New Hampshire

Investigations Into The Use Of Aldefluor® As An Alternative Method To Classify Leukemia In The Soft-Shell Clam Mya Arenaria., Katherine F. Norwood

Student Research Projects

The soft-shell clam species Mya arenaria is a common model organism in leukemia research. Currently, the typical method for classifying leukemic from non-leukemic specimens is based on visualization of cell concentration and morphology through light microscopy. This approach is highly qualitative, which makes identification of pre-leukemic and semi-leukemic specimens difficult. One more quantitative approach uses flow cytometry to measure levels of aldehyde dehydrogenase (ALDH) expression. In certain human tissues, such as breast tissue, high ALDH expression is a marker for identifying cancer stem cells. The enzyme assay ALDEFLUOR® can actively measure aldehyde dehydrogenase expression in viable cells, but the effectiveness ...


Targeted Knockout Of Beclin-1 Reveals An Essential Function In Ovary And Testis, Thomas R. Gawriluk 2014 University of Kentucky

Targeted Knockout Of Beclin-1 Reveals An Essential Function In Ovary And Testis, Thomas R. Gawriluk

Theses and Dissertations--Biology

An estimated 12% of couples worldwide are infertile. The contributing factor is approximately equal between men and women with nearly 25% diagnosed as idiopathic. Despite the increasing numbers of couples seeking assistance from infertility clinics, few molecular mechanisms have been identified for treatment. Autophagy is an evolutionarily conserved cellular process for bulk degradation and recycling of cytosolic components through the lysosome to maintain homeostasis. Several studies have observed increased levels of autophagy during ovarian folliculogenesis and gonadal steroidogenesis; however, no genetic studies to determine the significance of autophagy exist.

To investigate the function of autophagy in the ovary and testis ...


Pcb Effects On Development Of Behavior In Rats, Robert Witte 2014 Bowling Green State University

Pcb Effects On Development Of Behavior In Rats, Robert Witte

Honors Projects

Abstract

PCBs were originally used as industrial chemicals in many devices and applications, including electrical capacitors and transformers, lubricants, and sealants in buildings. PCBs have been investigated and their use has been slowly phased out starting in the U.S. in the 1970s. A worldwide ban was put on the use and manufacturing of PCB in 2001, however they remain in the food chain because of their lipophilic properties and their long half-life. Major sources of PCB include wild-life, fish, and other seafood. Individuals exposed to PCB during development can experience negative effects on neurobiological, cognitive, and behavior functioning in ...


The Effects That Liquid And Solid Cattle Manure Have On The Water Quality Of Drainage Ditches In Putnam County, Ohio, Janelle Horstman 2014 Bowling Green State University

The Effects That Liquid And Solid Cattle Manure Have On The Water Quality Of Drainage Ditches In Putnam County, Ohio, Janelle Horstman

Honors Projects

Lake Erie has experienced harmful algal blooms with increased frequency since the mid-1990s due to excess nutrients from Rivers, such as the Maumee River, and largely agricultural watersheds. Nonpoint source pollution from agriculture contributes to eutrophication, algal blooms, and the degradation of water quality. This creates stress on aquatic fauna, reduced aesthetic quality, odor, and limits of the water for usage of drinking, recreation, and industry. This research paper asks what the contributions of having access to manure application records, soil records, and information about antibiotics have on what is known about manure management and antibiotic resistance, which has been ...


The Effects Of Electrolyte Chemical Composition, Concentration And Ph On The Electrochemical Redox Reactions, Sea Choi, Xiao-Ying Yu 2014 California Polytechnic State University

The Effects Of Electrolyte Chemical Composition, Concentration And Ph On The Electrochemical Redox Reactions, Sea Choi, Xiao-Ying Yu

STEM Teacher and Researcher (STAR) Program Posters

Electrolyte composition, concentration, pH, ionic strength, and temperature have long been known to play a role in the interface of the electrode-electrolyte. However, detailed studies are not sufficient. Cyclic voltammetry (CV) is a widely used electrochemical technique for obtaining qualitative information in electroanalysis. CV is often the first experiment performed in an electrode surface in an electrochemical study. It measures the number of electrons transferred and intermediates formed during oxidation-reduction (redox) reactions by observing its current, intensity and position of peak potential with applied voltage. This is accomplished by using a three-electrode system including working electrode (WE), counter electrode (CE ...


The Roles Of Phenotypic Plasticity And Genotypic Specialization In High Altitude Adaptation, Danielle M. Tufts 2013 University of Nebraska - Lincoln

The Roles Of Phenotypic Plasticity And Genotypic Specialization In High Altitude Adaptation, Danielle M. Tufts

Dissertations and Theses in Biological Sciences

In vertebrates living at high altitude, arterial hypoxemia may be ameliorated by reversible changes in the oxygen-carrying capacity of the blood (regulated by erythropoiesis) and/or changes in blood–oxygen affinity (regulated by allosteric effectors of hemoglobin function). These hematological traits often differ between taxa that are native to different elevational zones, but it is often unknown whether the observed physiological differences reflect fixed, genetically based differences or environmentally induced acclimatization responses (phenotypic plasticity). Here, we report measurements of hematological traits related to blood–O2 transport in populations of deer mice (Peromyscus maniculatus) that are native to high- and ...


Developmental And Molecular Functions Of Plakophilin-3, William A. Munoz 2013 Texas Medical Center Library

Developmental And Molecular Functions Of Plakophilin-3, William A. Munoz

UT GSBS Dissertations and Theses (Open Access)

Plakophilin-3, the less studied member of the plakophilin-catenin subfamily, and the larger catenin family, binds directly to desmosomal cadherin cytoplasmic domains and enhances desmosome formation and stability. In mammals, plakophilin-3 is expressed at the highest levels in desmosome-enriched tissues such as epithelia, with the knock-out in mice producing corresponding reductions in ectodermal integrity. In tissue, cellular and intracellular contexts where plakophilin-3 is not at the desmosomal plaque, little is known about its functions in the cytoplasm or nucleus, where it also localizes.

My work employed embryos of the amphibian, Xenopus laevis, to examine plakophilin-3’s developmental roles. I first evaluated ...


Iron-Regulated Cyanobacterial Predominance And Siderophore Production In Oligotrophic Freshwater Lakes, Ryan J. Sorichetti 2013 Western University

Iron-Regulated Cyanobacterial Predominance And Siderophore Production In Oligotrophic Freshwater Lakes, Ryan J. Sorichetti

University of Western Ontario - Electronic Thesis and Dissertation Repository

The frequency and intensity of cyanobacterial blooms (cyanoblooms) is increasing globally. Contrary to existing phosphorus (P) and nitrogen (N) paradigms describing cyanobloom proliferation in eutrophic (nutrient-rich) freshwater lakes, many of the recent cyanobloom reports pertain to oligotrophic (nutrient-poor) freshwater lakes with no prior history of cyanobloom occurrence. There exists a critical research need to re-visit existing conceptual models, identify regulating factors currently unaccounted for and improve our ability to effectively detect and measure cyanobacterial toxins (cyanotoxins) in lakes. Iron (Fe) is required in nearly all pathways of cyanobacterial macronutrient use, though its direct role in regulating cyanobacterial biomass is not ...


Stellaris Fishing 20131125mon Part 1 Of 2, George McNamara 2013 The University of Texas

Stellaris Fishing 20131125mon Part 1 Of 2, George Mcnamara

George McNamara

Stellaris FISH dataset using three FISH probe sets. Slides courtesy of Biosearch Technologies,

https://www.biosearchtech.com/store/product.aspx?catid=224,318,324

see http://stellarisfish.smugmug.com/ for online gallery by Biosearch.

This experiment was to evaluate the crosstalk between the Biosearch fluorophores:

Quasar 570

CAL Fluor Red 610 (CFR 610)

Quasar 670

DAPI (DNA counterstain)

Autofluorescence (green, but sometimes showing up in other channels).

and our lab's Leica DMI6000 fluorescence microscope with Leica filter sets:

DAPI

GFP (L5)

Cy3 (N3)

Texas Red (TxRed2)

Cy5 (Y5)

I also acquired green channel and red channel with exciter filters ...


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