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280 full-text articles. Page 1 of 8.

Autoantibodies To The Ny-Eso-1 Tumor Antigen In Metastatic Melanoma: Sialylation Of The Fc Region Of Immunoglobulin G Induces Differential Expression Signatures Of Inflammatory Molecules During Dendritic Cell Differentiation And Maturation, Martin Oaks, Nathaniel Rein, John O. Richards, James Shaffer 2014 Aurora Health Care

Autoantibodies To The Ny-Eso-1 Tumor Antigen In Metastatic Melanoma: Sialylation Of The Fc Region Of Immunoglobulin G Induces Differential Expression Signatures Of Inflammatory Molecules During Dendritic Cell Differentiation And Maturation, Martin Oaks, Nathaniel Rein, John O. Richards, James Shaffer

Journal of Patient-Centered Research and Reviews

Purpose: We tested the hypothesis that different glycoforms of antibodies from patients with metastatic melanoma have different functional effects on human dendritic cell differentiation and maturation.

Methods: Antibodies to the cancer antigen NY-ESO-1 were affinity-purified from patients with melanoma and further fractionated into different glycoforms by lectin chromatography. Sialic acid-rich and sialic acid-poor fractions of these immunoglobulin G antibodies (IgG) were added to dendritic cell cultures during both differentiation and maturation, and the resulting cellular messenger RNA (mRNA) and culture supernatants were tested by microarray and enzyme-linked immunoassay for molecules related to inflammatory pathways.

Results: We identified unique mRNA and ...


Evaluation Of The Developmental Toxicity Of Lead In The Danio Rerio Body, Nicole M. Roy, Sarah DeWolf, Bruno Carneiro 2014 Sacred Heart University

Evaluation Of The Developmental Toxicity Of Lead In The Danio Rerio Body, Nicole M. Roy, Sarah Dewolf, Bruno Carneiro

Biology Faculty Publications

Lead has been utilized throughout history and is widely distributed and mobilized globally. Although lead in the environment has been somewhat mitigated, the nature of lead and its extensive uses in the past prohibit it from being completely absent from our environment and exposure to lead is still a public health concern. Most studies regarding lead toxicity have focused on the brain. However, little is found in the literature on the effects of lead in other tissues. Here, we utilize the zebrafish model system to investigate effects of lead exposure during early developmental time windows at 24, 48 and 72 ...


Wentworth Point History Bulletin # 10, Randy Lackovic 2014 The University of Maine

Wentworth Point History Bulletin # 10, Randy Lackovic

Darling Marine Center Historical Documents

This is a history of the marine and freshwater sciences activity at the University of Maine from 1865 - 1965.


Wentworth Point History Bulletin # 12, Randy Lackovic 2014 The University of Maine

Wentworth Point History Bulletin # 12, Randy Lackovic

Darling Marine Center Historical Documents

This history recounts the formation of the Darling Marine Center from 1963 - 1966.


Natural Egg Sac Clutch Size Of The Brown Widow Spider, Latrodectus Geometricus (Araneae: Theridiidae) In Southern California, Douglas W. R. Danielsen, Daniel E. Clarke, Stephan J. Valle, Amy A. Anselmo, Leonard S. Vincent, Richard S. Vetter 2014 Occidental College

Natural Egg Sac Clutch Size Of The Brown Widow Spider, Latrodectus Geometricus (Araneae: Theridiidae) In Southern California, Douglas W. R. Danielsen, Daniel E. Clarke, Stephan J. Valle, Amy A. Anselmo, Leonard S. Vincent, Richard S. Vetter

Bulletin of the Southern California Academy of Sciences

Abstract. We investigated egg sac clutch size of the brown widow spider, Latrodectus geometricus C. L. Koch, in southern California from June through October 2012 by counting eggs from 214 field collected egg sacs. Clutch size was fairly constant over this five-month period averaging 129.0 ± 51.0 eggs per sac with the only significant monthly difference being more eggs in September than June. Determining the relatively constant egg production for this period contributed knowledge to another project involving application of pesticide to brown widow egg sacs in attempted control of this newly established invasive pest species.


Annotated Bibliography: Attitudes Toward Animal Research (1998-2013), Erich Yahner 2014 Humane Society Institute for Science and Policy

Annotated Bibliography: Attitudes Toward Animal Research (1998-2013), Erich Yahner

Humane Education

No abstract provided.


Pi3k- And Mtor-Dependent Mechanisms Of Lapatinib Resistance And Resulting Therapeutic Opportunities, Samuel Brady 2014 Texas Medical Center Library

Pi3k- And Mtor-Dependent Mechanisms Of Lapatinib Resistance And Resulting Therapeutic Opportunities, Samuel Brady

UT GSBS Dissertations and Theses (Open Access)

Breast cancers with HER2 amplification represent 20-25% of breast cancer cases and are frequently responsive to the HER2 kinase inhibitor lapatinib, but generally for only short duration. We aimed to understand how breast cancers with HER2 amplification become resistant to lapatinib, in order to identify potential therapies that can overcome lapatinib resistance. To establish lapatinib resistance models we treated three HER2+ breast cancer cell lines with lapatinib for several months until they became lapatinib-resistant. We then compared lapatinib-sensitive (parental) cells with their lapatinib-resistant (LapR) counterparts to identify changes conferring lapatinib resistance. We found that activation of PI3K, specifically the p110α ...


A Comparison Of Fatigue During Cardiocerebral Resuscitation With Different Compression Rates Among Layperson And Professional Rescuers, Christopher Cassidy 2014 California Polytechnic State University

A Comparison Of Fatigue During Cardiocerebral Resuscitation With Different Compression Rates Among Layperson And Professional Rescuers, Christopher Cassidy

Master's Theses and Project Reports

Quality chest compressions during Cardiopulmonary Resuscitation (CPR) are vital to maintaining adequate perfusion of oxygenated blood to the organs of the body to sustain life. Over the years, the compression rate recommended in Basic Life Support (BLS)/ Advanced Cardiac Life Support (ACLS) protocols for the best possible outcome has risen, and with that increase there are questions regarding rescuer fatigue and the effectiveness of compressions. Layperson and professional rescuers, answering to an emergency, both maintain continuous chest compressions until advanced life support arrives. Depending on the location, this arrival time would most likely be longer than the two minute standard ...


Physical Activity In Individuals With Down Syndrome, Chelsea B. Spurgeon 2014 University of Tennessee, Knoxville

Physical Activity In Individuals With Down Syndrome, Chelsea B. Spurgeon

University of Tennessee Honors Thesis Projects

No abstract provided.


Energy Stress Causes Chaperones To Assemble Into Cytoplasmic Complexes, Kimberly J. Cope 2014 Texas Medical Center Library

Energy Stress Causes Chaperones To Assemble Into Cytoplasmic Complexes, Kimberly J. Cope

UT GSBS Dissertations and Theses (Open Access)

The majority of proteins require molecular chaperones to assist their folding into tertiary and quaternary structures. Certain stresses can compromise the weak hydrophobic forces responsible for these structures and lead to protein unfolding, misfolding, and aggregation. Aggregates of proteins are hallmarks of devastating diseases such as Alzheimer’s, Parkinson’s, and Huntington’s diseases. Fortunately, bacteria, plants, and fungi have a potent disaggregase, named Hsp104 in Saccharomyces cerevisiae. Recently, heat-induced aggregates, termed Q-bodies, were found to contain three molecular chaperones: Hsp70, Hsp104, and Hsp42. Their coalescence from small puncta into larger inclusions required Hsp104. During glucose deprivation, a stress that ...


Reactive Oxygen Species And P21 Waf1/Cip1 Are Both Essential For, Alison Fitzgerald 2014 Texas Medical Center Library

Reactive Oxygen Species And P21 Waf1/Cip1 Are Both Essential For, Alison Fitzgerald

UT GSBS Dissertations and Theses (Open Access)

Treatment of Head and Neck Squamous Cell Carcinoma, HNSCC, often requires multimodal therapy, including radiation therapy. The efficacy of radiotherapy in controlling locoregional recurrence, the most frequent cause of death from HNSCC, is critically important for patient survival. One potential biomarker to determine radioresistance is TP53, whose alterations are predictive of poor radiation response. The following work shows that the p53 transcriptional target, p21, is crucial in initiating and maintaining senescence in HNSCC, through its ability to regulate reactive oxygen species (ROS). With the use of a novel system to evaluate the impact of the TP53 missense mutations, we were ...


The Role Of Integrins In Support Of Pancreatic Function, Survival And Maturation, Matthew Riopel 2014 Western University

The Role Of Integrins In Support Of Pancreatic Function, Survival And Maturation, Matthew Riopel

University of Western Ontario - Electronic Thesis and Dissertation Repository

The pancreas is a glandular organ composed of endocrine and exocrine compartments. Integrins are cell adhesion molecules that connect cells to the extracellular matrix (ECM). Integrins modulate a variety of cellular effects, yet their mechanism of action in the developed pancreas is not well understood. Fibrin is a provisional ECM protein that contains ligands for integrin receptors. Fibrin is capable of supporting islet health, but it is unclear how fibrin exerts its effects. The objective of this thesis is to understand the role of integrin receptors on in vivo pancreatic cell function, survival, and proliferation. In addition, this thesis investigates ...


Evaluating Colostrum Quality With Niacin Supplementation In The Diet Of Dairy Cattle, Allison Marie Pike 2014 University of New Hampshire

Evaluating Colostrum Quality With Niacin Supplementation In The Diet Of Dairy Cattle, Allison Marie Pike

Student Research Projects

Dairy calves are born with a naive immune system, and must obtain passive immunity through ingestion of colostrum. Colostrum provides the newborn with antimicrobial proteins called immunoglobulins, such as immunoglobulin G (IgG), that protect the calf for the first few weeks of life. Calves that receive good quality colostrum (>50 g/L of IgG) are more likely to survive to adulthood. Niacin is a vasodilator that may increase blood flow to the mammary gland during production of colostrum in the mother. This experiment investigated the effect of niacin supplementation at a rate of 48 g/cow/day for 3 weeks ...


A Longitudinal View Of Primate Life In Two American Laboratories, Jonathan Balcombe 2014 Humane Society Institute for Science and Policy

A Longitudinal View Of Primate Life In Two American Laboratories, Jonathan Balcombe

Animal Research, Testing, and Experimentation

If representative of other facilities, our findings uncover serious welfare concerns for the wellbeing of primates kept in American research facilities. These animals face regular or chronic sources of pain and distress including noxious experimental and non‐experimental events and illness and injury; and severe and prolonged social disruptions. Pain relief is meager by comparison to that normally provided to humans, despite legislative requirements to minimize pain and distress and assume similarity to humans in terms of ability to experience pain and distress. Living environments are usually confinement indoors to a metal cage, often alone, and often with a minimum ...


Gain-Of-Function Mouse Models To Investigate Biological Roles Of Prmt6, Alessandra Di Lorenzo 2014 Texas Medical Center Library

Gain-Of-Function Mouse Models To Investigate Biological Roles Of Prmt6, Alessandra Di Lorenzo

UT GSBS Dissertations and Theses (Open Access)

Gain-of-function Mouse Models to Investigate Biological Roles of PRMT6

Alessandra Di Lorenzo, Ph.D. Candidate

Mentor: Dr. Mark T. Bedford

Protein Arginine Methyltransferase 6 (PRMT6) is the histone tail writer that methylates the H3R2 (arginine 2 of histone H3) residue, which counteracts the activating H3K4me3 mark. PRMT6 has been shown to behave both as transcriptional co-repressor (i.e. trhrombospondin-1, p21, p53), and co-activator (nuclear receptors). The co-repressor function of PRMT6 is likely the result of H3K4me3 antagonism, while the mechanism by which PRMT6 exerts its co-activator function has yet to be elucidated. PRMT6 is over-expressed in several types of tumors ...


Investigations Into Aldefluor As A Novel Method For Identifying Leukemia In Soft-Shell Clams, Katherine F. Norwood 2014 University of New Hampshire

Investigations Into Aldefluor As A Novel Method For Identifying Leukemia In Soft-Shell Clams, Katherine F. Norwood

Student Research Projects

The clam species Mya arenaria is a common model organism in leukemia research. The current method for classifying the degree of cancer progression is by examining cell morphology with light microscopy. This approach is highly qualitative, which makes differentiation of pre-leukemic and semi-leukemic individuals difficult. One quantitative approach that may differentiate individuals is based on levels of aldehyde dehydrogenase (ALDH) expression. The enzyme assay ALDEFLUOR® can actively measure ALDH expression in viable cells, but the effectiveness of certain protocol conditions is dependent upon the cell type.


Exploration Of Freshwater Cyanobacteria, Mariya Campbell 2014 Georgia State University

Exploration Of Freshwater Cyanobacteria, Mariya Campbell

Georgia State Undergraduate Research Conference

No abstract provided.


Salt Marsh Values In A Changing World: Examining Sea Level Rise On Tidal Marshes With A Surface Elevation Table, Sarah Martina Tierney 2014 University of New Hampshire

Salt Marsh Values In A Changing World: Examining Sea Level Rise On Tidal Marshes With A Surface Elevation Table, Sarah Martina Tierney

Student Research Projects

Rising seas are threatening coastal communities and putting added pressures on the natural environment. Sea level rise rates are increasing on a global scale (from 1.7 to 3.2 mm/yr). Salt marshes are not only intertidal habitats acutely influenced by sea levels, but they also provide key ecosystem services such as: buffers against storm surges, habitat for wildlife, carbon dioxide storage, and pollutant filtration. In New England, salt marshes have built at a rate of 1 to 2 mm annually over the past 4,000 years, which has kept pace with sea level rise. However, we do not ...


Investigations Into The Use Of Aldefluor® As An Alternative Method To Classify Leukemia In The Soft-Shell Clam Mya Arenaria., Katherine F. Norwood 2014 University of New Hampshire

Investigations Into The Use Of Aldefluor® As An Alternative Method To Classify Leukemia In The Soft-Shell Clam Mya Arenaria., Katherine F. Norwood

Student Research Projects

The soft-shell clam species Mya arenaria is a common model organism in leukemia research. Currently, the typical method for classifying leukemic from non-leukemic specimens is based on visualization of cell concentration and morphology through light microscopy. This approach is highly qualitative, which makes identification of pre-leukemic and semi-leukemic specimens difficult. One more quantitative approach uses flow cytometry to measure levels of aldehyde dehydrogenase (ALDH) expression. In certain human tissues, such as breast tissue, high ALDH expression is a marker for identifying cancer stem cells. The enzyme assay ALDEFLUOR® can actively measure aldehyde dehydrogenase expression in viable cells, but the effectiveness ...


The Effects Of Electrolyte Chemical Composition, Concentration And Ph On The Electrochemical Redox Reactions, Sea Choi, Xiao-Ying Yu 2014 California Polytechnic State University

The Effects Of Electrolyte Chemical Composition, Concentration And Ph On The Electrochemical Redox Reactions, Sea Choi, Xiao-Ying Yu

STEM Teacher and Researcher (STAR) Program Posters

Electrolyte composition, concentration, pH, ionic strength, and temperature have long been known to play a role in the interface of the electrode-electrolyte. However, detailed studies are not sufficient. Cyclic voltammetry (CV) is a widely used electrochemical technique for obtaining qualitative information in electroanalysis. CV is often the first experiment performed in an electrode surface in an electrochemical study. It measures the number of electrons transferred and intermediates formed during oxidation-reduction (redox) reactions by observing its current, intensity and position of peak potential with applied voltage. This is accomplished by using a three-electrode system including working electrode (WE), counter electrode (CE ...


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