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The Relationship Between Climate And Nesting Patterns In Two-Lined Salamanders Spanning The Northeast To Southeast United States, Jasmyne Blake-Sinclair 2021 Kennesaw State University

The Relationship Between Climate And Nesting Patterns In Two-Lined Salamanders Spanning The Northeast To Southeast United States, Jasmyne Blake-Sinclair

Symposium of Student Scholars

Two-lined salamanders (Eurycea bislineata species complex) are among the most ubiquitous amphibians in the Eastern United States. Because this group is geographically widespread and shows notable life history variation, it provides an opportunity to study the role of phylogeny and environment in shaping these differences. However, records of some important life history traits—such as clutch size and the date of oviposition—are scarce in the scientific literature. In this study, we gathered observations from published research, field notes, and citizen science databases like iNaturalist—a site where anyone can share natural history photographs and pinned geographical locations for other ...


Effect Of Carbon Monoxide Releasing Molecule 3 (Corm - 3) On Platelet Adhesion To Human Brain Microvascular Endothelial Cells, Najat S. El-Farra 2021 Western University

Effect Of Carbon Monoxide Releasing Molecule 3 (Corm - 3) On Platelet Adhesion To Human Brain Microvascular Endothelial Cells, Najat S. El-Farra

Undergraduate Student Research Internships Conference

Sepsis is characterized by the widespread inflammation of the body. Systemic inflammation activates and recruits inflammatory cells (e.g., leukocytes) and platelets to the affected organs.

During these inflammatory conditions, human brain microvascular endothelial cells (hBMEC) and platelets both upregulate adhesive molecules rendering platelets to adhere to hBMEC.

Although carbon monoxide is thought of as a toxic molecule to many, previous work shows its anti-inflammatory properties. Evidence has shown carbon monoxide-releasing molecules (e.g., CORM-3; that release small, non-toxic amounts of CO) can combat the effects of severe inflammation in several in vivo animal model.

In this current study, we ...


Overcoming Obstacles In Protein Expression In The Yeast Pichia Pastoris: Interviews Of Leaders In The Pichia Field, Zoe Ingram, Abha Patkar, Dahoon Oh, Kevin K. Zhang, Christina Chung, Joan Lin-Cereghino, Geoff P. Lin-Cereghino 2021 University of the Pacific

Overcoming Obstacles In Protein Expression In The Yeast Pichia Pastoris: Interviews Of Leaders In The Pichia Field, Zoe Ingram, Abha Patkar, Dahoon Oh, Kevin K. Zhang, Christina Chung, Joan Lin-Cereghino, Geoff P. Lin-Cereghino

Pacific Journal of Health

The yeast Pichia pastoris (also known as Komagataella pastoris) has been used for over 30 years to produce thousands of valuable, heterologous proteins, such as insulin to treat diabetes and antibodies to prevent migraine headaches. Despite its success, there are some common, stubborn problems encountered by research scientists when they try to use the yeast to produce their recombinant proteins. In order to provide those working in this field with strategies to overcome these common obstacles, nine experts in P. pastoris protein expression field were interviewed to create a written review and video (https://www.youtube.com/watch?v=Q1oD6k8CdG8 ...


Modulation Of Glucose Homeostasis By Nucleotide P2y2 Receptor And Biological Sex, Hailee Anne Marino 2021 Missouri State University

Modulation Of Glucose Homeostasis By Nucleotide P2y2 Receptor And Biological Sex, Hailee Anne Marino

MSU Graduate Theses

Recent insights into the pathological role of Nucleotide P2Y2 receptor suggest P2Y2R involvement in high fat diet-induced obesity and potentiates insulin resistance. However, these recent insights do not demonstrate how P2Y2R modulates glucose homeostasis under physiological conditions. Further, it remains unknown how sex biological factors influence P2Y2R receptor signaling in the regulation of glucose homeostasis. The research objective for the present study is to elucidate the novel roles of P2Y2 in fasting blood glucose and glucose tolerance (basal insulin sensitivity) under resting conditions in males and females. We expected that under physiological ...


Expression And Purification Of Proteins For A Structural Determination: Orf8, Paivy1, Paivy2, Sarah Fashinasi, Avery Moss 2021 Kennesaw State University

Expression And Purification Of Proteins For A Structural Determination: Orf8, Paivy1, Paivy2, Sarah Fashinasi, Avery Moss

Symposium of Student Scholars

Protein purification is a crucial procedure in order to analyze the behavior, structure, and function of a select protein. The Orf8 protein is an increasingly mutable accessory protein found within the SARS-CoV2 virus, however the complex and unpredictable nature of this protein has been met by its inability to be purified thus far. By purifying the model proteins, PaIVY1 and PaIVY2, we believe that we can denote a sound purification method to apply to the Orf8 protein. In our procedure, we transformed BL21(DE3)pLysS cells and inoculated them in LB media. The cells were then placed into a centrifuge ...


The Probability Of Success-Through Fault Tree Analytical Binomial Result, Tyleka RIddle 2021 Embry-Riddle Aeronautical University

The Probability Of Success-Through Fault Tree Analytical Binomial Result, Tyleka Riddle

Night Flight Journal

Prestigious Appeal Rule of Thumb (Ear Drum Plugs)

In truant fashion, with proven accidental probabilistic perception for proposed cued facial expression recognition and voice commands without filtering. The estimated cost analysis for design and development generated from the enhanced project in the word "no”: Additionally, an audio noise effect size (ES) for desired level of comprehensive sensory receptors in sound reference to noisy party syndrome. Sytoss, (2019) "Resulting in a cocktail party theory for chalkboard distinction and conversation with applied facial recognition. As well as, the enhanced project management tool, EPMT, to provide output and input determination for higher-level acoustic ...


Search For Palladin, An Actin-Associated Protein, In Pig Retinal Pigmented Epithelium And Its Role In Epithelial-Mesenchymal Transition, Katrina Powell 2021 Bellarmine University

Search For Palladin, An Actin-Associated Protein, In Pig Retinal Pigmented Epithelium And Its Role In Epithelial-Mesenchymal Transition, Katrina Powell

Undergraduate Theses

This study investigates the expression of Palladin, a phosphoprotein product of the PALLD gene, in the retinal pigmented epithelium (RPE). Palladin is an actin cross-linking protein and plays a role in cell adhesion and motility. Published reports have demonstrated that a down regulation of Palladin in colon cancer cells results in a reorganization of the actin cytoskeleton, causing the cells to lose their typical shape, become proliferative and migratory. This process is otherwise known as epithelial-mesenchymal transition (EMT). A similar EMT phenomenon is observed when the RPE is exposed to the vitreous humor in patients with proliferative vitreoretinopathy (PVR). In ...


Stage Of Estrous Cycle Influences Behavior After Social Stress, Alexander Jonah Rodriguez 2021 University of South Dakota

Stage Of Estrous Cycle Influences Behavior After Social Stress, Alexander Jonah Rodriguez

Honors Thesis

Since women are almost twice as likely to develop major depression compared to men, research into this and other potentially debilitating mood disorders using behavioral studies should involve females despite hormonal, physiological, and potential behavior changes due to reproductive cyclicity. Until recently, most studies exclusively used males, thus limiting results of previous research to half of the population. Using adult female C57BL/6N mice as model organisms, we exposed them to novel forms of social stress across five days which encompassed the four-day estrous cycle to determine if the stages of the estrous cycle had an impact on behavior when ...


Comparing Cognition In Women Between The Ages Of (18-40) And (40-70), Annalyn Smith 2021 University of Arkansas, Fayetteville

Comparing Cognition In Women Between The Ages Of (18-40) And (40-70), Annalyn Smith

Biological Sciences Undergraduate Honors Theses

Currently, 5.8 million Americans are living with Alzheimer’s disease (AD), which is a degenerative brain disease. It is currently the sixth-leading cause of death in the United States (Alzheimer’s Association, 2019). AD also possesses a financial burned on the United States, estimated to cost $290 billion in 2019. Sufferers of AD insidiously lose their memory, personality, and judgment abilities until there are no longer recognizable by their loved ones. Currently, the best chance suffers of AD have is to catch the disease early on to slow its effects. This is due to the fact that treatment can ...


Implementing Crispr-Cas9 Gene Editing System In Microbotryum., Stevana Reese Schauer 2021 University of Louisville

Implementing Crispr-Cas9 Gene Editing System In Microbotryum., Stevana Reese Schauer

College of Arts & Sciences Senior Honors Theses

Microbotryum dianthorum is a species of smut fungus which causes infection in plants in the Dianthus genus. The infection process is aided by small-secreted proteins known as effectors. Effectors may aid in the infection process because they are secreted by the fungus and can enter plant tissue, possibly to facilitate infection and/or manipulate the host. Thus, it is of interest to investigate the genes for these effectors to determine their role in the infection process. One method to study genes is through gene disruption, via the CRISPR/Cas9 gene editing system, but this has not yet been implemented in ...


Intermachine Reliability Of The Velotron During Wingate Anaerobic Testing, Caleb Christie 2021 Utah State University

Intermachine Reliability Of The Velotron During Wingate Anaerobic Testing, Caleb Christie

All Graduate Plan B and other Reports

Purpose: The purpose of this study was to evaluate inter-machine reliability of the Velotron electromagnetically-braked cycle ergometer for the Wingate anaerobic test (WAnT) with power-trained athletes. Methods: Anaerobic athletes participating in football or track and field at a National Collegiate Athletics Association Division I program were recruited for participation in the study. Each participant visited the laboratory 3 times (1 familiarization trial, 2 experimental trials) within 2 weeks and at least 24 hours between visits. Participants completed a “practice trial” on the first visit to familiarize themselves with the 30-second WAnT. The WAnT procedure included a 5-minute warmup at a ...


Validation Of A Novel Trpm8 Knockout Mouse Model, Bilan Mohamed 2021 University of Southern Maine

Validation Of A Novel Trpm8 Knockout Mouse Model, Bilan Mohamed

Thinking Matters Symposium

Recent studies suggest that the use of thermoregulatory treatments that affect brown fat may help curb obesity. However it is unknown how these treatments may affect bone homeostasis. Our work has focused on the transient receptor potential melastatin (TRPM8) protein, which is responsible for detecting colder temperatures in sensory neurons. Previous work within the Motyl laboratory has found that Trpm8 plays a role in bone acquisition. Mice with a global deletion of the Trpm8 gene have reduced trabecular bone volume fraction due to reduced bone formation by osteoblasts. However, it is unclear whether sensory neuron or osteoblast-mediated expression of Trpm8 ...


Importance Of Understanding Genetic Predisposition, Andrew Carter 2021 Southern Maine Community College

Importance Of Understanding Genetic Predisposition, Andrew Carter

Thinking Matters Symposium

Hypersensitivity reactions can be sudden and are often fatal. Many of these reactions occur as a result of allergies to prescribed medication. Adverse drug reactions or (ADR’s) were show in a recent study to affect over 6% of hospitalized patients and resulted in over 100,000 deaths a year in the US alone. This poster will review the importance of understanding genetic predisposition, through articles about possible genetic causes of allergies to penicillin and other beta-lactams. Changes in a specific HLA gene located on chromosome 6 showed a correlation to penicillin reactions in over 600,000 participants. Another study ...


Effect Of Temperature On Questing Activity Of Ixodes Scapularis In A Laboratory Setting, Molly Meagher 2021 University of Southern Maine

Effect Of Temperature On Questing Activity Of Ixodes Scapularis In A Laboratory Setting, Molly Meagher

Thinking Matters Symposium

Understanding deer tick (Ixodes scapularis) behavior is increasingly important due to the species’ northerly range expansion, and consequently, increased human contact. Deer ticks are a major public health concern, as they are the primary vector of Lyme disease and other serious diseases. Differences in questing behavior are of considerable interest as fluctuating weather due to climate change effect the species’ range expansion and time spent questing. Although previous research has examined temperature preferences of tick behavior, there are conflicting reports about direct effects on questing activity. Therefore, I addressed the relationship between temperature and questing behavior of adult deer ticks ...


Perivascular Adipose Tissue In Relation To Diet, Thermogenesis And Cardiovascular Health, Ginger Paquette, Caitlin Stieber, Ashely Soucy, Benjamin Tero, Lucy Liaw 2021 University of Southern Maine

Perivascular Adipose Tissue In Relation To Diet, Thermogenesis And Cardiovascular Health, Ginger Paquette, Caitlin Stieber, Ashely Soucy, Benjamin Tero, Lucy Liaw

Thinking Matters Symposium

Adipose tissue is a diverse and crucial component to vascular health due to its role in energy storage and heat production. The primary function of white adipose tissue (WAT) is energy storage while the function of mitochondria-rich brown adipose tissue (BAT) is heat production. Perivascular adipose tissue (PVAT), which surrounds blood vessels, contains both WAT and BAT adipocytes. Dietary calorie restriction is associated with increased lifespan with decreased adiposity. Increased prevalence of WAT-like PVAT, due to high-fat diets and obesity, leads to increased metabolic disfunction and cardiovascular-disease. We used a calorie-restriction model in C57BL6/J mice to test the hypothesis ...


Purple Variety Of Sweet Potato, Ming Gao 2021 Prairie View A&M University

Purple Variety Of Sweet Potato, Ming Gao

Datasets Collection

Purple sweet potatoes are versatile enough to be used in cupcakes as well as ice cream. However, when cooked in their natural state, they are dry and starchy, prompting most to use the purple sweet potatoes as flour for baked goods, or in some cases, for ice cream. Dr. Gao wants his variety of purple sweet potato to be more palatable. Gao earned the coveted USDA-National Institute of Food and Agriculture 1890 Institution Capacity Building Grant to support his research. The project will focus on developing a variety that is enjoyable in its natural state and is moist and tastefully ...


Interrogating The Functions Of The Histone H2a Repression Domain In Relation To Cell-Cycle Progression In Saccharomyces Cerevisiae, Taylor Nattress 2021 Susquehanna University

Interrogating The Functions Of The Histone H2a Repression Domain In Relation To Cell-Cycle Progression In Saccharomyces Cerevisiae, Taylor Nattress

Senior Scholars Day

In eukaryotic cells, DNA is wrapped around histone proteins to form the basic repeating unit of chromatin, the nucleosome core particle. Most nucleosomes consist of two copies each of histones: H2A and H2B in a heterodimer and histones H3 and H4 in a heterotetramer. Because of their intimate association with DNA, histones regulate all DNA-templated processes such as transcription, DNA replication, and DNA damage response and repair. Several studies have illustrated the importance of a small portion of the histone H2A amino terminal domain which regulates global transcription. This domain is responsible for the repression of ~4% of the yeast ...


S.O.S. For Covid-19 (Subjective And Objective Screening): Are Asymptomatic Cases Truly Without Warning Signs?, Nathan Hughes 2021 Wayne State Unviersity

S.O.S. For Covid-19 (Subjective And Objective Screening): Are Asymptomatic Cases Truly Without Warning Signs?, Nathan Hughes

Honors College Theses

This study investigates the usefulness of five objective signs, evaluates relationships between objective signs with symptoms of COVID-19, and assesses the accuracy of ambient forehead temperature with tympanic temperature. Data were collected at Wayne State University. Blood oxygen levels and blood pressure were measured via pulse oximeters and blood pressure cuff. Body temperature was measured with a thermometer via the temple and the forehead. Smell was tested with two scented markers. None of the 29 participants confirmed a positive COVID-19 test. Therefore, only relationships between vital sign measurements were reported. No significant correlations were revealed upon analysis. Infrared and temporal ...


The Effect Of A Single Dose Of Beetroot Juice On Speed, Strength, And Power In Healthy Recreationally Active Females., Maya A. Hammer, Ryan Kenney, Katie Price, Chandler Riley, Alyssa Riley, Courtney Merrill, Adam Pennell, Rachel Tan 2021 Pepperdine University

The Effect Of A Single Dose Of Beetroot Juice On Speed, Strength, And Power In Healthy Recreationally Active Females., Maya A. Hammer, Ryan Kenney, Katie Price, Chandler Riley, Alyssa Riley, Courtney Merrill, Adam Pennell, Rachel Tan

Seaver College Research And Scholarly Achievement Symposium

Nitrate-rich beetroot juice (BR) improves muscle contraction which is relevant for intense intermittent-type sports. However, few studies have examined the effect of BR ingestion on performance using sport-specific exercise protocols. Moreover, there is a scarcity of research that include female participants which limits real world application given that females potentially have different responses to BR ingestion. The purpose of the present study was to examine the effect of BR supplementation on speed, acceleration, strength and power before and after fatigue in females. In a double-blind, randomized crossover design, 15 recreationally active females consumed BR and nitrate-depleted placebo juice (PL) ~2 ...


The Effects Of Nitrate-Rich Beetroot Juice Supplementation On Nonverbal Executive Function In Healthy Recreationally Active Females, Chandler F. Riley, Alyssa Riley, Maya A. Hammer, Ryan Kenney, Katie Price, Courtney Merrill, Adam Pennell, Rachel Tan 2021 Pepperdine University

The Effects Of Nitrate-Rich Beetroot Juice Supplementation On Nonverbal Executive Function In Healthy Recreationally Active Females, Chandler F. Riley, Alyssa Riley, Maya A. Hammer, Ryan Kenney, Katie Price, Courtney Merrill, Adam Pennell, Rachel Tan

Seaver College Research And Scholarly Achievement Symposium

Nitrate-rich beetroot juice (BR) supplementation has been reported to preserve executive function (i.e. decision making and reaction time) before and after a simulated soccer match in recreationally active males, which may be due to enhanced cerebral blood flow. However, the literature examining the physiological response following BR ingestion in females is scarce, which hampers the extrapolation of results since physiological sex-differences may exist. Therefore, the purpose of this study was to assess if BR ingestion influenced executive function in an unfatigued and fatigued state in healthy recreationally active females. In a double-blind, randomized crossover design, 20 females consumed 140 ...


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