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Gain-Of-Function Mouse Models To Investigate Biological Roles Of Prmt6, Alessandra Di Lorenzo 2014 Texas Medical Center Library

Gain-Of-Function Mouse Models To Investigate Biological Roles Of Prmt6, Alessandra Di Lorenzo

UT GSBS Dissertations and Theses (Open Access)

Gain-of-function Mouse Models to Investigate Biological Roles of PRMT6

Alessandra Di Lorenzo, Ph.D. Candidate

Mentor: Dr. Mark T. Bedford

Protein Arginine Methyltransferase 6 (PRMT6) is the histone tail writer that methylates the H3R2 (arginine 2 of histone H3) residue, which counteracts the activating H3K4me3 mark. PRMT6 has been shown to behave both as transcriptional co-repressor (i.e. trhrombospondin-1, p21, p53), and co-activator (nuclear receptors). The co-repressor function of PRMT6 is likely the result of H3K4me3 antagonism, while the mechanism by which PRMT6 exerts its co-activator function has yet to be elucidated. PRMT6 is over-expressed in several types of tumors ...


Exploration Of Freshwater Cyanobacteria, Mariya Campbell 2014 Georgia State University

Exploration Of Freshwater Cyanobacteria, Mariya Campbell

Georgia State Undergraduate Research Conference

No abstract provided.


The Roles Of Phenotypic Plasticity And Genotypic Specialization In High Altitude Adaptation, Danielle M. Tufts 2013 University of Nebraska - Lincoln

The Roles Of Phenotypic Plasticity And Genotypic Specialization In High Altitude Adaptation, Danielle M. Tufts

Dissertations and Theses in Biological Sciences

In vertebrates living at high altitude, arterial hypoxemia may be ameliorated by reversible changes in the oxygen-carrying capacity of the blood (regulated by erythropoiesis) and/or changes in blood–oxygen affinity (regulated by allosteric effectors of hemoglobin function). These hematological traits often differ between taxa that are native to different elevational zones, but it is often unknown whether the observed physiological differences reflect fixed, genetically based differences or environmentally induced acclimatization responses (phenotypic plasticity). Here, we report measurements of hematological traits related to blood–O2 transport in populations of deer mice (Peromyscus maniculatus) that are native to high- and ...


Developmental And Molecular Functions Of Plakophilin-3, William A. Munoz 2013 Texas Medical Center Library

Developmental And Molecular Functions Of Plakophilin-3, William A. Munoz

UT GSBS Dissertations and Theses (Open Access)

Plakophilin-3, the less studied member of the plakophilin-catenin subfamily, and the larger catenin family, binds directly to desmosomal cadherin cytoplasmic domains and enhances desmosome formation and stability. In mammals, plakophilin-3 is expressed at the highest levels in desmosome-enriched tissues such as epithelia, with the knock-out in mice producing corresponding reductions in ectodermal integrity. In tissue, cellular and intracellular contexts where plakophilin-3 is not at the desmosomal plaque, little is known about its functions in the cytoplasm or nucleus, where it also localizes.

My work employed embryos of the amphibian, Xenopus laevis, to examine plakophilin-3’s developmental roles. I first evaluated ...


Iron-Regulated Cyanobacterial Predominance And Siderophore Production In Oligotrophic Freshwater Lakes, Ryan J. Sorichetti 2013 Western University

Iron-Regulated Cyanobacterial Predominance And Siderophore Production In Oligotrophic Freshwater Lakes, Ryan J. Sorichetti

University of Western Ontario - Electronic Thesis and Dissertation Repository

The frequency and intensity of cyanobacterial blooms (cyanoblooms) is increasing globally. Contrary to existing phosphorus (P) and nitrogen (N) paradigms describing cyanobloom proliferation in eutrophic (nutrient-rich) freshwater lakes, many of the recent cyanobloom reports pertain to oligotrophic (nutrient-poor) freshwater lakes with no prior history of cyanobloom occurrence. There exists a critical research need to re-visit existing conceptual models, identify regulating factors currently unaccounted for and improve our ability to effectively detect and measure cyanobacterial toxins (cyanotoxins) in lakes. Iron (Fe) is required in nearly all pathways of cyanobacterial macronutrient use, though its direct role in regulating cyanobacterial biomass is not ...


Stellaris Fishing 20131125mon Part 1 Of 2, George McNamara 2013 The University of Texas

Stellaris Fishing 20131125mon Part 1 Of 2, George Mcnamara

George McNamara

Stellaris FISH dataset using three FISH probe sets. Slides courtesy of Biosearch Technologies,

https://www.biosearchtech.com/store/product.aspx?catid=224,318,324

see http://stellarisfish.smugmug.com/ for online gallery by Biosearch.

This experiment was to evaluate the crosstalk between the Biosearch fluorophores:

Quasar 570

CAL Fluor Red 610 (CFR 610)

Quasar 670

DAPI (DNA counterstain)

Autofluorescence (green, but sometimes showing up in other channels).

and our lab's Leica DMI6000 fluorescence microscope with Leica filter sets:

DAPI

GFP (L5)

Cy3 (N3)

Texas Red (TxRed2)

Cy5 (Y5)

I also acquired green channel and red channel with exciter filters ...


Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, Rajesh Pati 2013 SelectedWorks

Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, Rajesh Pati

Rajesh Pati

A standard uniform protocol of regeneration is the prime and foremost prerequisite for not only improve the productivity, the production from the existing area but also development of transgenics in mango for various traits. Polyembryonic cultivars are good rootstock because of moderate canopy, tolerant to draught, salt, environmental stress (wind, high temperature, air pollution), fungal disease (anthracnose, powdery mildew, gummosis) and hoppers.


Genetic Transformation Of Bael (Aegle Marmelos Corr.), Rajesh Pati 2013 SelectedWorks

Genetic Transformation Of Bael (Aegle Marmelos Corr.), Rajesh Pati

Rajesh Pati

Aegle marmelos Corr. is better known for its medicinal values rather than its edible quality. Every part of the plant viz. leaves, fruits, roots, and the bark are used for treating various diseases related to heart, stomach, bacterial, fungal, viral, worms, fertility, fever and cosmetics. Marmelosin is one of the major compounds (coumarin), used to treat various diseases. Till date, there are no varieties developed for commercial orchards of Bael and hence, regarded as one among the underutilized fruits of India. Therefore, there is an urgent need for breeding new and cultivable varieties in Aegle marmelos. But the problem for ...


Micropropagation In Fruit Crops, Rajesh Pati 2013 SelectedWorks

Micropropagation In Fruit Crops, Rajesh Pati

Rajesh Pati

Micropropagation is a proven means of producing millions of identical plants under a controlled and aseptic condition, independent of seasonal constraints. It not only provides economy of time and space but also gives greater output and allows further augmentation of elite disease free propagules. India is homeland of many important fruit crops such as Indian gooseberry (Emblica officinalis Gaertn), bael (Aegle marmelos Corr.), Guava (, Psidium guajava), jamun or black plum (Syzygium cuminii L. Skeels.), Mango (Mangifera indica) and Papaya (Carica papaya).


In Vitro Plant Regeneration And Genetic Assessment Among Regenerates Using Molecular Markers In Aegle Marmelos Corr., Rajesh Pati 2013 SelectedWorks

In Vitro Plant Regeneration And Genetic Assessment Among Regenerates Using Molecular Markers In Aegle Marmelos Corr., Rajesh Pati

Rajesh Pati

Bael (Aegle marmelos Corr.) is an important medicinal fruit tree. The fruit pulp contains marmelosin, which is a laxative, diuretic, is being used in many patented drugs in India. Micropropagation technology can be gainfully employed in mass multiplication of improved bael varieties. We have developed micropropagation protocol of bael through shoot bud culture. It was imperative to test genetic fidelity of micropropagated plants using molecular markers (RAPD, DAMD and ISSR).


Raman Spectroscopy As A Teaching Tool In The Forensic Science And Chemistry Courses, Zhaohua Dai, Elmer-Rico E. Mojico 2013 Pace University

Raman Spectroscopy As A Teaching Tool In The Forensic Science And Chemistry Courses, Zhaohua Dai, Elmer-Rico E. Mojico

Cornerstone 3 Reports : Interdisciplinary Informatics

No abstract provided.


The Effect Of Articulation And Word-Meaning On Gait And Balance In People With Parkinson’S Disease, Kevin Wood 2013 Western University

The Effect Of Articulation And Word-Meaning On Gait And Balance In People With Parkinson’S Disease, Kevin Wood

University of Western Ontario - Electronic Thesis and Dissertation Repository

Performing two tasks simultaneously is ubiquitous in everyday life, and the resulting interference may degrade performance on one or both of the tasks. This is potentially important, as diminished performance of a postural task places an individual at a greater risk for falling, especially in a movement impaired population such as individuals with Parkinson’s disease (PD). Many secondary tasks have been shown to reduce the performance of gait and balance, but to date only one study has investigated the effects of a verbal secondary task that systematically controls articulatory, cognitive, and linguistic demands. Previous research suggested that these components ...


Coccidia Of Gerbils From Mongolia, Ethan T. Jensen 2013 University of Nebraska - Lincoln

Coccidia Of Gerbils From Mongolia, Ethan T. Jensen

Dissertations and Theses in Biological Sciences

In this study, gerbils collected in the Mongolia over the summers of 2009-2012 were examined for coccidia. In total, 171 gerbils of three species from 22 localities were examined for coccidia. Coccidian oocysts were identified from 21 gerbils, but those found in 1 of those gerbils were probably pseudoparasites of the host from which they were recovered. From the remaining 20 gerbils, 7 morphotypes of Eimeria and 1 morphotype of Isospora were identified. Four of the 7 morphotypes of Eimeria were attributed to new species which were described in this study. In addition, 10 previously described species of Eimeria were ...


Life Support: Long Term Storage Of Solid Waste In An Enclosed Membrane System, Emmy O. Trieu, Michael Flynn, Rocco Mancinelli 2013 California Polytechnic State University

Life Support: Long Term Storage Of Solid Waste In An Enclosed Membrane System, Emmy O. Trieu, Michael Flynn, Rocco Mancinelli

STEM Teacher and Researcher (STAR) Program Posters

In deep space missions, maintaining life support is of the utmost priority. In such a closed system, human waste must be stored and treated. Simulated feces (ersatz) will be inoculated with microbes representing normal fecal flora, mixed with urine brine and shredded refuse of products typically used in space missions. Composting methods often use alternating layers of waste with scraps of carboniferous materials (finely shredded refuse). By preparing membrane bags with a homogenized ersatz and carboniferous refuse mixture and membrane bags with alternating layers of ersatz and carboniferous refuse, it may be possible to monitor anaerobic thermophillic digestion of the ...


Characterization Of Tcl1-Tg:P53-/- Mice That Resemble Human Chronic Lymphocytic Leukemia With 17p-Deletion, Jinyun Liu 2013 Texas Medical Center Library

Characterization Of Tcl1-Tg:P53-/- Mice That Resemble Human Chronic Lymphocytic Leukemia With 17p-Deletion, Jinyun Liu

UT GSBS Dissertations and Theses (Open Access)

Chronic lymphocytic leukemia (CLL) is the most common adult leukemia in the United Statesand Europe. CLL patients with deletion of chromosome 17p, where the tumor suppressor p53 gene is located, often develop a more aggressive disease with poor clinical outcomes. However, the underlying mechanism remains unclear. In order to understand the underneath mechanism in vivo, I have recently generated mice with Eu-TCL1-Tg:p53-/- genotype and showed that these mice develop aggressive leukemia that resembles human CLL with 17p deletion. The Eu-TCL1-Tg:p53-/- mice developed CLL disease at 3-4 months, significantly earlier than the parental ...


Histological And Biochemical Changes In Aegle Marmelos Corr. Before And After Acclimatization, Rajesh Pati 2013 SelectedWorks

Histological And Biochemical Changes In Aegle Marmelos Corr. Before And After Acclimatization, Rajesh Pati

Rajesh Pati

Maximum mortality of micropropagated plants occur during acclimatization phase because plantlets undergo rapid and extreme changes in physiological functioning, histological and biochemical changes. In order to investigate the actual reason of this limitation, test samples were collected at different stages of micropropagation of Aegle marmelos Corr. (In vitro stage, acclimation stage). The biochemical result showed that micropropagated plantlets produced significantly low total chlorophyll (0.042 mg/g fresh weight), reducing sugar (3.227%), NR activity (1.353 NO2/h/g fresh weight) and but higher protein (0.048 μg/g) during in vitro phase. The in vitro raised plants showed ...


Flash4 Dark Reference Images, George McNamara 2013 University of Miami

Flash4 Dark Reference Images, George Mcnamara

George McNamara

Hamamatsu FLASH4.0 dark reference images, acquired with 10 second exposure times, no light to camera. Camera offset (set by Hamamatsu( is ~100 (the average intensity of the first image is always ~1 intensity level higher - an odd feature, but trivial in practice for a 16-bit camera).

George McNamara, Ph.D.

Single Cells Analyst at L.J.N. Cooper Lab

University of Texas M.D. Anderson Cancer Center


Lurking In The Lab: Analysis Of Data From Molecular Biology Laboratory Instruments, Jen Ferguson 2013 University of Massachusetts Medical School

Lurking In The Lab: Analysis Of Data From Molecular Biology Laboratory Instruments, Jen Ferguson

Journal of eScience Librarianship

OBJECTIVE: This project examined primary research data files found on instruments in a molecular biology teaching laboratory. Experimental data files were analyzed in order to learn more about the types of data generated by these instruments (e.g. file formats), and to evaluate current laboratory data management practices.

SETTING: This project examined experimental data files from instruments in a teaching laboratory at Brandeis University.

METHODOLOGY: Experimental data files and associated metadata on instrument hard drives were captured and analyzed using Xplorer2 software. Formats were categorized as proprietary or open, and characteristics such as file naming conventions were noted. Discussions with ...


The Effects Of Blocking Hippocampal Acetylcholine On Meal Onset In Male Rats, Jasmine Nicole Gentry 2013 Georgia State University

The Effects Of Blocking Hippocampal Acetylcholine On Meal Onset In Male Rats, Jasmine Nicole Gentry

Georgia State Undergraduate Research Conference

No abstract provided.


Video Codec Performance (Excel Spreadsheet), George McNamara 2013 University of Miami

Video Codec Performance (Excel Spreadsheet), George Mcnamara

George McNamara

Video codec performance (Excel spreadsheet). Movie was made in 2005-2006 when I worked at City of Hope National Medical Center. VTLF refers to Video Timelapse Light Facility. Videos were outputted from MetaMorph as AVI files. Personally, I always recommend uncompressed video files fro scientific uses. I also encourage posting the original scientific data format (ex. .lsm, .zvi, .lif, .stk).


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