Stellaris Fishing 20131125mon Part 1 Of 2, 2013 The University of Texas
Stellaris Fishing 20131125mon Part 1 Of 2, George Mcnamara
Stellaris FISH dataset using three FISH probe sets. Slides courtesy of Biosearch Technologies,
see http://stellarisfish.smugmug.com/ for online gallery by Biosearch.
This experiment was to evaluate the crosstalk between the Biosearch fluorophores:
CAL Fluor Red 610 (CFR 610)
DAPI (DNA counterstain)
Autofluorescence (green, but sometimes showing up in other channels).
and our lab's Leica DMI6000 fluorescence microscope with Leica filter sets:
Texas Red (TxRed2)
I also acquired green channel and red channel with exciter filters ...
Raman Spectroscopy As A Teaching Tool In The Forensic Science And Chemistry Courses, 2013 Pace University
Raman Spectroscopy As A Teaching Tool In The Forensic Science And Chemistry Courses, Zhaohua Dai, Elmer-Rico E. Mojico
Cornerstone 3 Reports : Interdisciplinary Informatics
No abstract provided.
The Effect Of Articulation And Word-Meaning On Gait And Balance In People With Parkinson’S Disease, 2013 Western University
The Effect Of Articulation And Word-Meaning On Gait And Balance In People With Parkinson’S Disease, Kevin Wood
University of Western Ontario - Electronic Thesis and Dissertation Repository
Performing two tasks simultaneously is ubiquitous in everyday life, and the resulting interference may degrade performance on one or both of the tasks. This is potentially important, as diminished performance of a postural task places an individual at a greater risk for falling, especially in a movement impaired population such as individuals with Parkinson’s disease (PD). Many secondary tasks have been shown to reduce the performance of gait and balance, but to date only one study has investigated the effects of a verbal secondary task that systematically controls articulatory, cognitive, and linguistic demands. Previous research suggested that these components ...
Coccidia Of Gerbils From Mongolia, 2013 University of Nebraska - Lincoln
Coccidia Of Gerbils From Mongolia, Ethan T. Jensen
Dissertations and Theses in Biological Sciences
In this study, gerbils collected in the Mongolia over the summers of 2009-2012 were examined for coccidia. In total, 171 gerbils of three species from 22 localities were examined for coccidia. Coccidian oocysts were identified from 21 gerbils, but those found in 1 of those gerbils were probably pseudoparasites of the host from which they were recovered. From the remaining 20 gerbils, 7 morphotypes of Eimeria and 1 morphotype of Isospora were identified. Four of the 7 morphotypes of Eimeria were attributed to new species which were described in this study. In addition, 10 previously described species of Eimeria were ...
Life Support: Long Term Storage Of Solid Waste In An Enclosed Membrane System, 2013 California Polytechnic State University
Life Support: Long Term Storage Of Solid Waste In An Enclosed Membrane System, Emmy O. Trieu, Michael Flynn, Rocco Mancinelli
STEM Teacher and Researcher (STAR) Program Posters
In deep space missions, maintaining life support is of the utmost priority. In such a closed system, human waste must be stored and treated. Simulated feces (ersatz) will be inoculated with microbes representing normal fecal flora, mixed with urine brine and shredded refuse of products typically used in space missions. Composting methods often use alternating layers of waste with scraps of carboniferous materials (finely shredded refuse). By preparing membrane bags with a homogenized ersatz and carboniferous refuse mixture and membrane bags with alternating layers of ersatz and carboniferous refuse, it may be possible to monitor anaerobic thermophillic digestion of the ...
Flash4 Dark Reference Images, 2013 University of Miami
Flash4 Dark Reference Images, George Mcnamara
Hamamatsu FLASH4.0 dark reference images, acquired with 10 second exposure times, no light to camera. Camera offset (set by Hamamatsu( is ~100 (the average intensity of the first image is always ~1 intensity level higher - an odd feature, but trivial in practice for a 16-bit camera).
George McNamara, Ph.D.
Single Cells Analyst at L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Lurking In The Lab: Analysis Of Data From Molecular Biology Laboratory Instruments, 2013 University of Massachusetts Medical School
Lurking In The Lab: Analysis Of Data From Molecular Biology Laboratory Instruments, Jen Ferguson
Journal of eScience Librarianship
OBJECTIVE: This project examined primary research data files found on instruments in a molecular biology teaching laboratory. Experimental data files were analyzed in order to learn more about the types of data generated by these instruments (e.g. file formats), and to evaluate current laboratory data management practices.
SETTING: This project examined experimental data files from instruments in a teaching laboratory at Brandeis University.
METHODOLOGY: Experimental data files and associated metadata on instrument hard drives were captured and analyzed using Xplorer2 software. Formats were categorized as proprietary or open, and characteristics such as file naming conventions were noted. Discussions with ...
The Effects Of Blocking Hippocampal Acetylcholine On Meal Onset In Male Rats, 2013 Georgia State University
The Effects Of Blocking Hippocampal Acetylcholine On Meal Onset In Male Rats, Jasmine Nicole Gentry
Georgia State Undergraduate Research Conference
No abstract provided.
Video Codec Performance (Excel Spreadsheet), 2013 University of Miami
Video Codec Performance (Excel Spreadsheet), George Mcnamara
Video codec performance (Excel spreadsheet). Movie was made in 2005-2006 when I worked at City of Hope National Medical Center. VTLF refers to Video Timelapse Light Facility. Videos were outputted from MetaMorph as AVI files. Personally, I always recommend uncompressed video files fro scientific uses. I also encourage posting the original scientific data format (ex. .lsm, .zvi, .lif, .stk).
Pubspectra Tattletales, 2013 The University of Texas
Pubspectra Tattletales, George Mcnamara
Tattletales for Multiplex Fluorescent Reporters in Single Cells for Metabolomics
As of April 2013: L.J.N. Cooper & D.A. Lee Cellular Immunotherapy Lab, University of Texas M.D. Anderson Cancer Center, Houston, TX
Email: firstname.lastname@example.org, email@example.com
Tattletales is my concept for spatial multiplexing many fluorescent protein (FP) biosensors in the same live cell. For example, there are excellent FP biosensors to Ca++ ions, pH, glucose, ribose, glutamine, glutamate, ATP, redox, ROS, pyruvate, cAMP, cGMP, IP3, PI(3,4,5)P3, cell cycle indicators (Fucci2), PKA, PKC, photsphatases, caspase(s) [1, 2]. However, these are typically used one biosensor per experiment, due in part to flooding the cell with soluble biosensor. That is, conventionally, either a metabolite (glucose) reporter or a signal transduction (Ca++) reporter can be imaged. By flooding the cell with the reporter, signal to noise ratio is compromized by autofluorescence.
Tattletales takes advantage of spatial multiplexing to both increase the number of different reporters, and improve signal to noise ratio by localizing each biosensor to a small volume. I started with the observation by Robinett et al  who localized 512 GFP-nls-LacI to a 256 LacO array as a 200 nm diameter diffraction limited spot (nuclear background due to overexpression). Many thousands of DNA binding proteins, of known sequence specifities, exist (LacI, TetR, GalR, etc for cell line studies; ZF-FPs, TALE-FPs to STRs, telomere repeat binding factors-FPs, etc for primary cells) and can be fused (as cDNAs) to different fluorescent proteins and FP biosensors.
Many biosensors are available as affinity series [1, 4], now enabling extended dynamic range. I realized that spatial multiplexing of many DNA binding protein-reporters by localizing to different spots in the cell nucleus and distinguished by combinatorial addressing, where N address colors provide 2^N addresses (example, 3 colors is 2^3 = 8 combinations). Multiplexing ...
The Problem Of Too Many Statistical Tests: Subgroup Analyses In A Study Comparing The Effectiveness Of Online And Live Lectures, 2013 University of South Florida
The Problem Of Too Many Statistical Tests: Subgroup Analyses In A Study Comparing The Effectiveness Of Online And Live Lectures, David M. Lane
The more statistical analyses performed in the analysis of research data, the more likely it is that one or more of the conclusions will be in error. Multiple statistical analyses can occur when the sample contains several subgroups and the researchers perform separate analyses for each subgroup. For example, separate analyses may be done for different ethnic groups, different levels of education, and/or for both genders. Media reports of research frequently omit information on the number of subgroup analyses performed thus leaving the reader with insufficient information to assess the validity of the conclusions. This article discusses the problems ...
Biology Department Newsletter, No. 3, 2013 Sacred Heart University
Filtering Efficiency And Feeding Mechanisms Of Daphnia Pulex On Microcystis Aeruginosa And Nannochloropsis, 2012 University of New Hampshire
Filtering Efficiency And Feeding Mechanisms Of Daphnia Pulex On Microcystis Aeruginosa And Nannochloropsis, Elizabeth Orlowicz
The filtering and feeding rate of Daphnia pulex from the Old Durham Reservoir, Durham, NH, were measured to determine the feeding efficiency on different concentrations of non-toxic Nannochloropsis (Class Eustigmatophyceae) and toxigenic cyanobacteria Microcystis aeruginosa. Direct observations of thoracic appendage beats and post-abdominal rejections were also measured at 5*105 and 106 cells mL-1 concentrations of Nannochloropsis and M. aeruginosa to examine the feeding mechanisms of the D. pulex. In the presence of increasing Nannochloropsis concentrations, the filtering rates decreased and the feeding rates increased. When exposed to M. aeruginosa, both the filtering and feeding rates decreased ...
Investigation Into Potential Cross-Resistance Of Enterococcus Faecalis Og1rf Induced By Adaptation To Nacl And Subsequent Sds Challenge, 2012 University of Tennessee, Knoxville
Investigation Into Potential Cross-Resistance Of Enterococcus Faecalis Og1rf Induced By Adaptation To Nacl And Subsequent Sds Challenge, Vidisha Singh
University of Tennessee Honors Thesis Projects
No abstract provided.
Halloween 2012 Jack O'Lanterns Trick Or Treat, 2012 University of Miami
Halloween 2012 Jack O'Lanterns Trick Or Treat, George Mcnamara
Halloween 2012 makes trick or treating more visual and interactive than in past years.
the download is a ZIP file containing three files.
Print out the (unnumbered) image on as large and nice printer paper as possible - I used glossy 44" wide here in Miami (University of Miami, MillerSchool of Medicine, Calder Library, Biomedical Communications dept - I also made another print on "fabric", also 44" wide to take with me to an HHMI Janelia Farm conference on 'turning images into knowledge' that ends on Oct 31 - might stay up for a second conference, "GFP..." that start Nov 4).
The other ...
A Call For Transparent Reporting To Optimize The Predictive Value Of Preclinical Research, 2012 University of Massachusetts Medical School
A Call For Transparent Reporting To Optimize The Predictive Value Of Preclinical Research, Story C. Landis, Marc Fisher
Neurology Publications and Presentations
The US National Institute of Neurological Disorders and Stroke convened major stakeholders in June 2012 to discuss how to improve the methodological reporting of animal studies in grant applications and publications. The main workshop recommendation is that at a minimum studies should report on sample-size estimation, whether and how animals were randomized, whether investigators were blind to the treatment, and the handling of data. We recognize that achieving a meaningful improvement in the quality of reporting will require a concerted effort by investigators, reviewers, funding agencies and journal editors. Requiring better reporting of animal studies will raise awareness of the ...
An Agent–Based Computational Model For Bank Formation And Interbank Networks, 2012 McMaster University
An Agent–Based Computational Model For Bank Formation And Interbank Networks, Omneia R.H. Ismail
Open Access Dissertations and Theses
The aim of this thesis is to study the role of banking in society and the effect of the
interbank market on the performance of the banking system.
It starts by reviewing
several studies conducted on empirical banking networks and highlighting their salient
features in the context of modern network theory. A simulated network resembling the
characteristics documented in the empirical studies is then built and its resilience is
analyzed with a particular emphasis in documenting the crucial role played by highly
It is our belief that the study of systemic risk and contagion in a banking system ...
Mcnamara 20120831fri-20120904tue Cosmic Ray Particles By Ccd Imaging, 2012 University of Miami
Mcnamara 20120831fri-20120904tue Cosmic Ray Particles By Ccd Imaging, George Mcnamara
McNamara 20120831Fri-20120904Tue Cosmic Ray Particles by CCD imaging.zip contains image files in support of a Microscopy Today article - please see
Light Scattering Study Of Elongated Particles: From Inorganic Nanorice To Polypeptide Micelles, 2012 Cleveland State University
Light Scattering Study Of Elongated Particles: From Inorganic Nanorice To Polypeptide Micelles, Philip Dee
Undergraduate Research Posters 2012
Utilizing the powerful experimental technique of Dynamic Light Scattering (DLS) for size characterization of anisotropic particles can be extremely misleading. Unfortunately, this point is often not realized by researchers who strive for particle sizing of nanoparticles in suspensions. We present a consistent analysis of DDLS results on FeOOH nanorice and outline the potential difficulties and challenges of DDLS application for polypeptide micelles.
Protein Body Formation In Stable Transgenic Plants Of Nicotiana Tabacum Expressing Elastin-Like Polypeptide And Hydrophobin Fusion Proteins, Sonia P. Gutierrez
Electronic Thesis and Dissertation Repository
Plants are recognized as an efficient and inexpensive system to produce valuable recombinant proteins. However, the use of plants still faces two main limitations: the low accumulation levels of some recombinant proteins and the lack of efficient protein purification methods. Two fusion partners, elastin-like polypeptides (ELP) and hydrophobin I (HFBI) were found to increase the accumulation of recombinant proteins and induce the formation of protein bodies (PBs) when targeted to the ER in transient expression assays. In this study I examined the effect of these tags in stable transgenic plants of two Nicotiana tabacum cultivars when fused to green fluorescent ...