Laboratory and Basic Science Research Commons^™

Fish Tissue Digestion And Microplastic Polymer Identification Protocol Using Open-Source Ftir Database, Shawn L. Kissinger, Vick-Ariel Privert, Jason Bystriansky, Kyle A. Grice

DePaul Discoveries

Microplastic (MP) contamination in aquatic ecosystems poses significant concerns for environmental and public health, as seafood represents a critical food source worldwide. Despite increasing evidence of MP presence in marine organisms, efficient and accessible detection methods remain essential for characterizing contamination patterns and informing regulatory frameworks. This study extracted MPs from brain, gill, intestine, liver, and muscle tissues of gilt-head bream (Sparus aurata) and European sea bass (Dicentrarchus labrax), two commercially important species from the Gulf of Cádiz, Spain, using a NaOH–HNO₃ chemical digestion protocol. Isolated particles were characterized by Fourier Transform Infrared (FTIR) spectroscopy, with …

Contribution Of Animals To Biomedical Advancements In The Current Era: A Narrative Review, Leena Rajathy Port Louis, Mani Rajarathinam, Prithiviraj Nagarajan, Divya Sekar, Nisha Perumal

The Thai Journal of Pharmaceutical Sciences

Animal research has played a major role in the development of vaccinations and therapies for illnesses such as tetanus, polio, meningitis, hepatitis, and Severe Acute Respiratory Syndrome Coronavirus 2. For every prescription drug available today, animal testing and research have contributed immensely. This narrative review aims to explore the contribution of animals to biomedical advancements in the current era, which involved a search of peer-reviewed literature using electronic databases such as Scopus, Web of Science, and PubMed. In current biomedical research, animals help develop new treatments for long-term illnesses such as cancer, HIV, and Alzheimer’s. Animals also aid in the …

Diversifying Clinical Trial Participation: Leveraging Digital Health Innovations And Community Outreach For Recruitment And Retention Improvements, Blake Aaron Lambert

Doctoral Dissertations and Projects

The purpose of this qualitative study was to explore how digital tools and community outreach can improve recruitment and retention in clinical trials, particularly among underserved populations. This study examined key barriers to participation, the potential benefits and limitations of digital tools, and strategies to engage underrepresented groups. Surveys were conducted with healthcare professionals (HCPs) and community members to gather perspectives on participation in clinical research. NVivo was used to code and analyze data. The analysis identified themes related to cultural barriers and trust, awareness, logistical barriers and accessibility, structural barriers and relevance, digital tools, and engagement. Participants were expected …

Supramolecular Assembly In Short Peptide Systems For Selective Metabolite Recognition And Drug Nanoencapsulation, Maithreyi Ramakrishnan

Dissertations, Theses, and Capstone Projects

Short peptides can form adaptive supramolecular assemblies, and understanding how minimal sequences organize around neurometabolites or hydrophobic cancer drugs enables the rational design of functional materials. This thesis combines molecular dynamics with experimental validation to establish design rules linking peptide sequence to emergent structure and function. Chapter 1 outlines the molecular determinants governing peptide assembly. Chapter 2 reviews computational workflows that reveal sequence-dependent conformations and supramolecular organization. Chapter 3 applies these principles to Dynamic Peptide Libraries which identify tetrapeptides that selectively interact with neurometabolites. Chapter 4 extends the same interaction-driven framework to design tryptophan-rich pentapeptides that co-assemble with kinase inhibitors …

Determining Cave Presence And Diet Variation Of The Ozark Big-Eared Bat (Corynorhinus Townsendii Ingens) From Guano Dna, Lark E. Sybrant

ATU Theses and Dissertations 2021 - Present

The Ozark big-eared bat (Corynorhinus townsendii ingens; OBEB) is federally endangered in Arkansas, where it persists in a few small, geographically restricted populations within the Ozark National Forest. Although the region contains many caves that appear suitable for OBEB, expanded survey efforts are needed to locate additional roost sites for monitoring and protection. Effective conservation also requires a clear understanding of their distribution and diet. Traditional cave surveys can be disruptive and often miss cryptic species, and earlier diet studies based on manually sorting prey remains provided only coarse taxonomic resolution and overlooked soft‑bodied insects. To address these …

Building Critical Thinking And Ai Literacy Through The Topic Development Process In Kinesiology, Brandi Row Lazzarini

Educational Practices in Kinesiology

The purpose of this teaching and learning resource is to provide a process for developing academic and meta-cognitive skills in students as they engage meaningfully in literature analysis and practice deploying generative artificial intelligence (genAI) effectively and responsibly. Additionally, while gaining critical AI literacy, students will develop the skills needed to advance their critical thinking and avoid overreliance on generative AI. This resource is unique in the specificity of its activities, which promote a healthy perspective on how genAI can be used in the early stages of learning about a topic. It also helps students practice the skills needed to …

The Longevity Of Dna From Bloodstains And Its Forensic Implications, Nadia Walter

Senior Honors Theses

Human DNA is valuable forensic evidence, helping to solve a wide range of cases. It is often sourced from biological fluids found at a scene such as blood, saliva, semen, and urine. Many different factors can influence the longevity of DNA sourced from bloodstains, including the type of substrate, storage conditions, and weather. The quality of a DNA sample from a bloodstain found at a crime scene may influence its admissibility in court.

Original research was done on a surgical table from the last major battle of the American Civil War, extracting DNA from latent bloodstains. Some full profiles were …

Atomoxetine Enhances Specific Dimensions Of Cognitive Performance In A Rodent Strategy Shifting Assay, Jessica Ivanov

Rowan-Virtua Research Day

Title: Atomoxetine enhances specific dimensions of cognitive performance in a rodent strategy shifting assay

Authors: Jessica Ivanov, Christopher P. Knapp, Barry D. Waterhouse, Rachel L. Navarra

Objective: Cognitive flexibility, a domain of executive function, supports adaptive shifts in behavior in response to changing environmental demands and task contingencies. Deficits in this capacity are common to neuropsychiatric disorders such as Attention Deficit Hyperactivity Disorder (ADHD), where improper regulation of the catecholamine transmitters dopamine (DA) and norepinephrine (NE) within the prefrontal cortex (PFC) has been implicated. Atomoxetine (ATX), a selective NE transporter (NET) inhibitor, elevates levels of NE and DA in the …

Beat Behind The Bark: Investigating The Effects Of Music Tempo On Canine Physiological And Psychological States, Marley Lanier

Honors Theses

This study explores how the tempo of music, measured in beats per minute (BPM), affects dogs' heart rate and oxytocin levels, adding to past research that shows that music affects physiological and behavioral states in dogs. Being able to understand these effects holds practical implications for improving overall well-being in canines in environments such as homes, shelters, and clinical environments. Using a within-subjects design, the dogs were exposed to slow and fast versions of one instrumental track, along with a white noise baseline condition before each randomized condition. Heart rate was recorded continuously throughout the session, and oxytocin saliva samples …

The Beat Behind The Bark: Investigating The Effects Of Music Tempo On Canine Physiological And Psychological States, Marley Lanier

Poster Presentations

Music is a universal component of human culture that has been demonstrated influencing emotional, cognitive, and physiological responses, specifically heart rate and hormonal responses (Mehr et al., 2019; Trappe, 2012). Recently, this research has expended to explore how music may affect non-human animals, particularly dogs, due to their roles as therapy animals, service animals, and human companions (Lindig et al., 2020; Gee et al., 2021). Being able to understand how music influences canine stress and relaxation hold important applications in environments such as shelters, veterinary clinics, and at home. One important feature of music is tempo, or beats per minute …

Exploring Student Sensemaking During A Collaborative Biology Lab, Rebecca Kollipara

School of Natural Resources: Dissertations, Theses, and Student Research

Collaborative learning environments offer valuable opportunities for students to construct scientific understanding through dialogue, yet the conditions that support sustained conceptual reasoning in group settings remain incompletely understood. While prior research has identified discourse moves associated with productive collaboration, less attention has been paid to how those moves function together across turns and conceptual boundaries, or how individual student characteristics shape their emergence. This thesis examines collaborative sensemaking in an undergraduate General Biology II course, drawing on two complementary analyses of student discourse during a bryophyte life-cycle modeling activity.

Chapter 1 investigates how student groups participated in sensemaking and how …

Optimizing Itpa R178c Assays Via Hplc, Jared J. Reisnouer, Wally D. Pines

2026 Symposium

Two of the major nucleotide bases in DNA and RNA (Adenine and Guanine) are derived from the purine Inosine Monophosphate (IMP). IMP may occasionally form the noncanonical nucleotide Inosine Triphosphate (ITP) within the cell and become incorporated into DNA during replication, leading to potentially lethal errors. To combat this, human cells produce the “housekeeping” enzyme Inosine Triphosphatase (ITPA) to revert ITP to IMP. A mutation of this protein that replaces the 178th amino acid Arginine with Cysteine (R178C) is associated with a fatal infantile encephalopathy. Previous assessments of enzyme-substrate binding and catalysis for ITPA variants have been run at a …

Short Lab Report- Presentation, Wroud Saleh, Yevgeniy Grigoryev

Open Educational Resources

No abstract provided.

Glioblastoma Multiforme: A Pathologic Breakdown Of One Of Neuro-Oncology's Greatest Challenges, Rosemary Westphal, Ms

Student Papers, Posters & Projects

Glioblastoma multiforme is the most common adult primary tumor with a grim median survival time of 14.6 months. It is characterized by rapid progression, diffuse infiltration, and rather poor clinical outcomes. The WHO classifies GBM as a grade 4 diffuse astrocytic tumor with distinctive histopathological features including high mitotic activity, marked cellular atypia, microvascular proliferation, and necrosis. Advances in molecular diagnostics have further classified GMB into IDH-wild type and IDH-mutant subtypes, with important therapeutic and prognostic implications. Key genetic alterations, such as EGFR amplification, PTEN loss, TP53 mutations, TERT promoter mutations, and MGMT methylations, have been identified as contributing to …

Perspective In Biomedical Sciences Field: Education And Career, Fitra Adi Prayogo, Mellyaning Oktaviani Sonya Kirana Sari, Isy Royhanaty, Poppy Fansisca Amelia, Heni Wijayanti, Ni Nyoman Maryaningtyas Adinatha, Dyah Wulandari, Anto Budiharjo

Chulalongkorn Medical Journal

Abstract

Background: The biomedical sciences represent a dynamic and multidisciplinary field pivotal to advancing human health through research, education, and innovation.

Objective(s): This review explores the evolving landscape of biomedical education and career pathways, driven by technological advancements, interdisciplinary integration, and global health demands.

Methods: We examine the distinctions between biomedical science, biomedicine, and biomedical engineering, highlighting their historical origins and contemporary roles. The education and career trajectories of biomedical laboratory scientists (BLS) are analysed, emphasising diverse competencies, core curricula, and the impact of regional policies on professional qualifications. Emerging trends and ethical/regulatory challenges are discussed.

Result(s): Key trends include …

Effects Of Phosphorus Addition On Fungal Colonization In Little Bluestem, Isabel M. Zanartu, Alexx Nicholas, Mckenzie Goodwyn, Connor Morozumi

The Cardinal Edge

Little Bluestem (Schizachyrium scoparium) is a North American perennial grass that is able to tolerate high heat and droughts. This is possible by its extensive underground root network that taps deep into the ground for resources. Within the same soil systems are fungal endophytes, which are symbionts that live within plant tissues including roots. Fungal endophytes can affect host growth and nutrient acquisition, but the functional role these endophytes play in plant health may be dependent on the abiotic conditions the plant is facing. In the modern era, one large driver of abiotic conditions is synthetic fertilizer use which alters …

Targeting Il-1 Signaling In Recessive Dystrophic Epidermolysis Bullosa: Improved Survival And Pathogenesis In Rdeb Mouse Model, Rahim Hirani

NYMC Student Theses and Dissertations

Recessive dystrophic epidermolysis bullosa (RDEB) is a devastating inherited blistering disorder caused by biallelic mutations in COL7A1, resulting in deficiency of type VII collagen (C7) and loss of dermal–epidermal adhesion. Beyond mechanical fragility, RDEB is characterized by chronic inflammation, progressive fibrosis, impaired wound healing, severe pain, and an exceptionally high risk of aggressive cutaneous squamous cell carcinoma. Emerging transcriptomic and cytokine profiling studies have identified interleukin-1 alpha (IL-1α) as a dominant and temporally upstream inflammatory mediator in RDEB skin. The central hypothesis of this dissertation is that IL-1α signaling functions as a key initiator and amplifier of inflammation-driven pathology …

Physical And Chemical Study Of Interaction In The Urea–Valine System For The Creation Of Modified Liquid Nitrogen Fertilizers, Suvonkul Erkhanovich Nurmonov, Saydullo Khamidovich Azimov

Chemical Technology, Control and Management

This study reports the results of a comprehensive physicochemical investigation of the urea–L-valine binary system, conducted to validate its potential as a modified base for liquid nitrogen fertilizers.

By utilizing the methods of isomolar series, visual-polythermal analysis, X-ray phase (XRD) and single-crystal X-ray diffraction (SC-XRD), synchronous thermal analysis (TGA/DTA), and FT-IR spectroscopy, it was determined that the interaction between components in both aqueous solutions and the solid phase results in the formation of molecular associates stabilized by hydrogen bonds, without the formation of new stoichiometric compounds.

The concentration and temperature limits of the system's stability were defined, revealing a reduction …

Design And Functionalization Of A Hierarchical Cu-Btc/Zeolite A4 Composite With Tannic Acid For High-Performance Heavy Metal Removal: Synergistic Adsorption Mechanisms, Kinetic Insights, And Reusability Evaluation, Samar A. Salim

Nanotechnology Research Centre

This study presents the development of a novel multifunctional adsorbent Cu-BTC (HKUST-1)/Zeolite A4 composite functionalized with tannic acid—for the efficient removal of toxic heavy metal ions (Pb²⁺, Cd²⁺, Cr(VI)) from aqueous solutions. The composite was synthesized via a solvothermal process and systematically characterized by XRD, FTIR, SEM-EDS, and BET surface area analysis. The resulting material exhibited a high specific surface area (1050 m²/g), a hierarchical pore structure, and a homogeneous distribution of active sites. Batch adsorption experiments revealed superior adsorption capacities of 220 mg/g for Pb²⁺, 180 mg/g for Cd²⁺, and 150 mg/g for Cr(VI) at pH 6, surpassing those …

Classification Of Venomous Snakes In Thailand Based On The Melting Temperature Specificity Of Target Genes Using Sybr Green Real-Time Pcr, Sunutcha Suntrarachun, Tanapong Tawan, Lawan Chanhome

The Thai Journal of Veterinary Medicine

The SYBR Green real-time PCR was developed for the rapid and accurate classification of major venomous snakes in Thailand. The method relies on melting temperature (Tm) discrimination of target genes from mitochondrial ribosomes. Short single-stranded primers were designed to specifically amplify synthetic DNA of venomous snakes in a single reaction (singleplex), producing distinct melting peaks. Neurotoxic snakes such as cobras (Naja spp.), king cobra (Ophiophagus hannah) and kraits (Bungarus spp.) exhibited melting peaks ranging from 81.00 to 86.00°C, whereas hematotoxic snakes, including the Siamese Russell's viper (Daboia siamensis), Malayan pit viper (Calloselasma rhodostoma …