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Characterization Of An 18f-Growth Hormone Secretagogue Probe For Positron Emission Tomography Imaging Of The Growth Hormone Secretagogue Receptor, Ahmed Abbas 2017 The University of Western Ontario

Characterization Of An 18f-Growth Hormone Secretagogue Probe For Positron Emission Tomography Imaging Of The Growth Hormone Secretagogue Receptor, Ahmed Abbas

Electronic Thesis and Dissertation Repository

Cardiovascular disease affects 1.6 million Canadians, of whom one-third have heart failure (HF). HF is diagnosed by imaging investigations and detection of circulating biomarkers. Most of the current imaging strategies study morphologic and gross functional changes, but fall short of imaging molecular abnormalities associated with HF. Biomarkers offer molecular targets; however, clinical biomarkers circulate systemically and are not cardiac-specific. Thus, there is critical need for a biomarker that is endogenous to myocardial tissues. The growth hormone secretagogue receptor 1a (GHSR1a), which binds the hormone ghrelin, is expressed by cardiomyocytes and is elevated in HF patients. This study characterized the ...


Characterization Of Zic2 As An Oncoprotein In Prostate Cancer, Keira C. Davis 2017 Clark Atlanta University

Characterization Of Zic2 As An Oncoprotein In Prostate Cancer, Keira C. Davis

Electronic Theses & Dissertations Collection for Atlanta University & Clark Atlanta University

The field of prostate cancer research is in need of biological markers that predict which cancers do not need treatment, those that can be treated successfully with a localized treatment and more specific cases in which patients are likely to have an aggressive form of cancer that will require more aggressive surgical and chemotherapeutic treatments. ZIC2 is one of five members of a family of proteins that play critical roles in neural crest and mesoderm growth in normal embryonic brain development and in the adult cerebellum of vertebrates. Found throughout the animal kingdom, ZIC1-5 genes encode five distinct ZIC proteins ...


B7h6: A Cancer Biomarker For The Development Of Novel Immunotherapy Approaches, Mariana Phillips 2017 Seton Hall University

B7h6: A Cancer Biomarker For The Development Of Novel Immunotherapy Approaches, Mariana Phillips

Seton Hall University Dissertations and Theses (ETDs)

Cancer-based immunotherapy has led the evolution of biologics that can stimulate immune responses towards tumor eradication. The synthesis of small to intermediate size molecules with the targeting and effector functions of mAb may represent a novel class of immunotherapeutics that may overcome the limitations of their biological counterparts.Towards this objective, B7H6 has been identified as a protein ligand localized on the cell surface of transformed tumor cells. B7H6 binds specifically to the activating receptor NKp30, constitutively expressed on all resting and active NK cells. Upon ligand:receptor binding, B7H6 triggers NK cell activation and release of chemokines and pro-inflammatory ...


Mrna Decay Pathways Use Translation Fidelity And Competing Decapping Complexes For Substrate Selection, Alper Celik 2017 University of Massachusetts Medical School

Mrna Decay Pathways Use Translation Fidelity And Competing Decapping Complexes For Substrate Selection, Alper Celik

GSBS Dissertations and Theses

mRNA decay is an important step in gene regulation, environmental responsiveness, and mRNA quality control. One such quality control pathway, Nonsense-mediated mRNA Decay (NMD), targets transcripts whose translation terminates prematurely. However, the scope and the defining features of NMD-targeted transcripts remain elusive. To address these issues, we re-evaluated the genome-wide expression of annotated transcripts in yeast cells harboring deletions of the UPF1, UPF2, or UPF3 genes. The vast majority of NMD-regulated transcripts are normal-looking protein-coding mRNAs. Our bioinformatics analyses reveal that this set of NMD-regulated transcripts generally have lower translational efficiency, lower average codon optimality scores, and higher ratios of ...


Immunology Of The Gray Short-Tailed Opossum During Pregnancy And Prenatal Development, Victoria L. Hansen 2017 University of New Mexico

Immunology Of The Gray Short-Tailed Opossum During Pregnancy And Prenatal Development, Victoria L. Hansen

Biology ETDs

Vertebrate species that bear live young have overcome the evolutionary challenge of maintaining both a functional adaptive immune system and viviparous life cycle. In normal pregnancy, viviparous mothers are able sustain and nourish a fetus that is genetically half non-self without mounting an immunological attack. In 1953 Sir Peter Medawar brought attention to the “enigma of the fetal graft” and immunologists have been puzzling out the intricacies of fetal tolerance ever since. Although viviparity has evolved in all jawed vertebrate lineages aside from Aves, the vast majority of reproductive immunology research has been limited to eutherian mammals. There are insights ...


Denoising Tandem Mass Spectrometry Data, Felix Offei 2017 East Tennessee State Universtiy

Denoising Tandem Mass Spectrometry Data, Felix Offei

Electronic Theses and Dissertations

Protein identification using tandem mass spectrometry (MS/MS) has proven to be an effective way to identify proteins in a biological sample. An observed spectrum is constructed from the data produced by the tandem mass spectrometer. A protein can be identified if the observed spectrum aligns with the theoretical spectrum. However, data generated by the tandem mass spectrometer are affected by errors thus making protein identification challenging in the field of proteomics. Some of these errors include wrong calibration of the instrument, instrument distortion and noise. In this thesis, we present a pre-processing method, which focuses on the removal of ...


Elucidating The Effects Of Thiamethoxam Neonicotinoid On Honey Bee Learning Using The Proboscis Extension Response, David J. Shepherd 2017 East Tennessee State University

Elucidating The Effects Of Thiamethoxam Neonicotinoid On Honey Bee Learning Using The Proboscis Extension Response, David J. Shepherd

Undergraduate Honors Theses

In this study, the effects of the neonicotinoid pesticide, thiamethoxam, are examined through the Proboscis Extension Response (PER) in honey bees (Apis mellifera). PER is a form of classical conditioning applied to honey bees through scent and reward association which quantifies learning rates. Results between groups treated with thiamethoxam did not differ significantly from untreated control groups. Potential reasons for these results are discussed. The method and experimental apparatus for testing the PER assay are also discussed.


Assessing The Rate And Extent Of Transgenerational Acclimation And Adaptation To Ocean Warming, Abby C. Nease 2017 Nova Southeastern University

Assessing The Rate And Extent Of Transgenerational Acclimation And Adaptation To Ocean Warming, Abby C. Nease

Theses and Dissertations

A primary goal of climate change research is to determine if species will be able to persist in a warmer environment. Most studies predict climate change will cause many species to become extinct. However, these predictions are based on experiments where only a single life stage or generation of a species was exposed to predicted future conditions (i.e. shock treatments), and thus overlook the possibility of species adapting or acclimatizing to new environmental conditions over multiple generations. As a result, current projections of species persistence through climate change are likely to overestimate species extinction. In this study, the rate ...


E,E-Farnesol Inhibits Swarming Motility In Burkholderia Cepacia Through Rhamnolipid Production, Stephanie E. Nicholls, Alayna N. Sanderson, Andrea P. Schwartz, Lauren E. Ward, Jessica N. Weisensee, Molly Yandrofski, Tracy L. Collins 2017 Cedarville University

E,E-Farnesol Inhibits Swarming Motility In Burkholderia Cepacia Through Rhamnolipid Production, Stephanie E. Nicholls, Alayna N. Sanderson, Andrea P. Schwartz, Lauren E. Ward, Jessica N. Weisensee, Molly Yandrofski, Tracy L. Collins

The Research and Scholarship Symposium

Burkholderia cepacia and Candida albicans both exhibit cell-to-cell communication through the use of quorum-sensing molecules (QSM) known as autoinducers. E,E-farnesol is a QSM produced by C. albicans which regulates its conversion from yeast to mycelium. Because there is a positive correlation between the presence of B. cepacia and C. albicans in the lungs of individuals with cystic fibrosis (CF), we examined whether E,E-farnesol had an effect on swarming motility in B. cepacia. Swarming motility was inhibited when B. cepacia was exposed to 250 µM of E,E-farnesol. In addition, there was a 26.8% decrease in rhamnolipid production ...


Spatial And Temporal Variability Of Water Quality Parameters In Cedar Lake (Cedarville, Ohio), Laura A. Cashman, William A. Smith, Shannon L. Swicker, Abigail L. Williams, Mark A. Gathany 2017 Cedarville University

Spatial And Temporal Variability Of Water Quality Parameters In Cedar Lake (Cedarville, Ohio), Laura A. Cashman, William A. Smith, Shannon L. Swicker, Abigail L. Williams, Mark A. Gathany

The Research and Scholarship Symposium

Cedar Lake is a man-made lake that is central to Cedarville University’s campus. This focal point of the campus is a source of aesthetic appeal and beauty of Cedarville University. The lake ranges in depth from 0.6-3.7m, is 140m by 150m across, an approximate volume of 40,000 m3 and demonstrates normal capabilities to support fish and other life. There is, however, some concern over Cedar Lake’s level of productivity as undesirable algae blooms are common during warmer months. In October and November 2016 we began to assess the spatial and temporal variability of water ...


Cichlid Behavioral Ecology: Streams Of Costa Rica, Ali Saheb, Erfan Ranjbar, Shannon Dieffenderfer, yazmeen Almutairri, nicole williams-ugula, Ella Brook Gray, Adel Pereira 2017 GSU

Cichlid Behavioral Ecology: Streams Of Costa Rica, Ali Saheb, Erfan Ranjbar, Shannon Dieffenderfer, Yazmeen Almutairri, Nicole Williams-Ugula, Ella Brook Gray, Adel Pereira

Georgia State Undergraduate Research Conference

No abstract provided.


Student Perceptions Of A Project-Based Laboratory Environment, Montana Nowak, Nikita Burrows, Suazette Mooring 2017 Georgia State University

Student Perceptions Of A Project-Based Laboratory Environment, Montana Nowak, Nikita Burrows, Suazette Mooring

Georgia State Undergraduate Research Conference

No abstract provided.


Oxidation Of Substituted Catechols At The Air-Water Interface: Production Of Carboxylic Acids, Quinones, And Polyphenols, Elizabeth A. Pillar, Marcelo I. Guzman 2017 University of Kentucky

Oxidation Of Substituted Catechols At The Air-Water Interface: Production Of Carboxylic Acids, Quinones, And Polyphenols, Elizabeth A. Pillar, Marcelo I. Guzman

Chemistry Faculty Publications

Anthropogenic activities contribute benzene, toluene, and anisole to the environment, which in the atmosphere are converted into the respective phenols, cresols, and methoxyphenols by fast gas-phase reaction with hydroxyl radicals (HO(•)). Further processing of the latter species by HO(•) decreases their vapor pressure as a second hydroxyl group is incorporated to accelerate their oxidative aging at interfaces and in aqueous particles. This work shows how catechol, pyrogallol, 3-methylcatechol, 4-methylcatechol, and 3-methoxycatechol (all proxies for oxygenated aromatics derived from benzene, toluene, and anisole) react at the air-water interface with increasing O3(g) during τc ≈ 1 μs contact time and contrasts their ...


Quantitative Evaluation Of Clinostomum Marginatum Distribution In The Fish Host (Perca Flavescens): Investigation Of The Impact On Functional Performance, Doiba KI Eshofonie, Precious Freeman 2017 Lynchburg College

Quantitative Evaluation Of Clinostomum Marginatum Distribution In The Fish Host (Perca Flavescens): Investigation Of The Impact On Functional Performance, Doiba Ki Eshofonie, Precious Freeman

Student Scholar Showcase

Doiba Eshofonie & Precious Freeman

Mentor: Takashi Maie

2017 Student Scholar Showcase

Quantitative evaluation of Clinostomum marginatum distribution in the fish host (Perca flavescens): Investigation of the impact on functional performance

Clinostomum marginatum is a trematode commonly found in freshwater fish. The adult C. marginatum stays in its definitive avian host’s mouth and throat. It releases eggs upon contact in water and the eggs hatch into a free-swimming miracidia, which infects an aquatic snail as the first intermediate host. The miracidia, then, develops into a cercaria and leaves the snail to find a fish as its second intermediate host. The ...


Inquiry Into Perilipin-5a Expression In Triacylglycerol Rich Vs Normal Fed Mouse Tissue, Kobi Agyepong 2017 Otterbein University

Inquiry Into Perilipin-5a Expression In Triacylglycerol Rich Vs Normal Fed Mouse Tissue, Kobi Agyepong

Honors Thesis Projects

The steep rise in both childhood and adult obesity over the past three decades has moved to the forefront of public consciousness in recent years. This development has generated a marked increase in general health awareness and lifestyle changes for a vast number of individuals, most notably in the form of increased physical activity and diet alterations. The latter point is especially salient in a biochemical context, because of the myriad factors that can result in “fat accumulation”. Chief among these factors is the Perilipin 5A gene, (known as PLIN5A) which encodes the protein Perilipin 5A of the Perilipin family ...


Investigating The Effects Of Pre-Laboratory Videos With Associated Questions On The Performance And Overall Laboratory Experience Of Organic Chemistry Students, Shareef Gamal Mohamed Kotb 2017 Merrimack College

Investigating The Effects Of Pre-Laboratory Videos With Associated Questions On The Performance And Overall Laboratory Experience Of Organic Chemistry Students, Shareef Gamal Mohamed Kotb

Health Sciences Student Work

Background: Teachers struggle communicating ideas to students (Moreno et al, 2001). One of the aspects of teaching that’s most concerning is when students experience cognitive overload: the total effort exerted by working memory to grasp new information (Mayer, et al, 2002). The educational community found that introducing multimedia in learning minimizes cognitive overload (Mayer, et al, 2002). Little research has related to undergraduate chemistry classes, specifically the relationship between organic chemistry pre-laboratory multimedia and student learning. Purpose: The purpose of this capstone is to investigate the subjective efficacy of integrating pre-laboratory videos for lab preparation in an undergraduate organic ...


The Efficacy And Safety Of Six-Weeks Of Pre-Workout Supplementation In Resistance Trained Rats, Justin P. Canakis 2017 College of William and Mary

The Efficacy And Safety Of Six-Weeks Of Pre-Workout Supplementation In Resistance Trained Rats, Justin P. Canakis

Undergraduate Honors Theses

Pre-workout supplements (PWSs) contain a cocktail of ingredients that are marketed to increase energy levels, endurance, and muscle power. PWSs are not regulated by the Food and Drug Administration (FDA) and have a history of causing adverse side effects. The goal of this study is to independently analyze the efficacy and safety of Pro Supps Mr. Hyde (MH) in resistance trained rats. Data suggest that six weeks of pre-workout supplementation in rats undergoing resistance training results in modest efficacy with improvement in amount lifted but no change in muscle mass. Urinary creatine tests and vascular reactivity tests were selected as ...


Reactivity Of Ketyl And Acetyl Radicals From Direct Solar Actinic Photolysis Of Aqueous Pyruvic Acid, Alexis J. Eugene, Marcelo I. Guzman 2017 University of Kentucky

Reactivity Of Ketyl And Acetyl Radicals From Direct Solar Actinic Photolysis Of Aqueous Pyruvic Acid, Alexis J. Eugene, Marcelo I. Guzman

Chemistry Faculty Publications

The variable composition of secondary organic aerosols (SOA) contributes to the large uncertainty for predicting radiative forcing. A better understanding of the reaction mechanisms leading to aerosol formation such as for the photochemical reaction of aqueous pyruvic acid (PA) at λ ≥ 305 nm can contribute to constrain these uncertainties. Herein, the photochemistry of aqueous PA (5-300 mM) continuously sparged with air is re-examined in the laboratory under comparable irradiance at 38° N at noon on a summer day. Several analytical methods are employed to monitor the time series of the reaction, including (1) the derivatization of carbonyl (C═O) functional ...


Pulmonary Surfactant Fortified With Cath-2 As A Novel Therapy For Bacterial Pneumonia, Brandon J. Baer 2017 University of Western Ontario

Pulmonary Surfactant Fortified With Cath-2 As A Novel Therapy For Bacterial Pneumonia, Brandon J. Baer

Western Research Forum

Background: Bacterial pneumonia is a leading cause of death worldwide, with high mortality rates persisting even after antibiotic treatment. Current treatments for pneumonia involve administration of antibiotics, however after the bacteria are killed they release toxic substances that induce inflammation and lung dysfunction. Host defense peptides represent a potential solution to this problem through their ability to down regulate inflammation. However, effective delivery to the lung is difficult because of the complex branching structure of the airways. My study addresses this delivery problem by using exogenous surfactant, a pulmonary delivery vehicle capable of improving spreading of these peptides throughout the ...


Assessment Of Olfactory-Based Social Recognition: Designing A Paradigm For Marmosets, Stephanie Womack 2017 University of Nebraska at Omaha

Assessment Of Olfactory-Based Social Recognition: Designing A Paradigm For Marmosets, Stephanie Womack

Student Research and Creative Activity Fair

Navigation of the social world depends largely on one’s responsiveness to social stimuli and information from a wide range of senses can be used to discriminate between individuals. Mammals use several sensory modes to communicate and respond to their surroundings, but olfaction is the dominant sense across most species. Chemosignals provide a wealth of information including sex, age, reproductive status, and individual identity. Currently, assessment of olfaction is largely limited to the habituation-dishabituation paradigm or the two-choice discrimination task used primarily in rodents. These paradigms are limited by their reliance on inherent reward of interaction with stimuli without providing ...


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