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3,769 full-text articles. Page 1 of 133.

Identification And Characterization Of Heat Shock Transcription Factor 1 Isoforms In Orange-Spotted Grouper (Epinephelus Coioides), Tingyu Wang 2016 National Cheng Kung University

Identification And Characterization Of Heat Shock Transcription Factor 1 Isoforms In Orange-Spotted Grouper (Epinephelus Coioides), Tingyu Wang

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


Igt And Igd From Sea Bass (Dicentrarchus Labrax): Localization Of Expressing And Immunoreactive Cells In Lymphoid Tissues., giuseppe scapigliati Prof., Simona Picchietti PhD, Francesco Buonocore PhD, Noelia Nuñez Ortiz, Valentina Stocchi, Laura Guerra PhD, Elisa Randelli PhD 2016 University of Tuscia, Dept. DIBAF, Viterbo, Italy

Igt And Igd From Sea Bass (Dicentrarchus Labrax): Localization Of Expressing And Immunoreactive Cells In Lymphoid Tissues., Giuseppe Scapigliati Prof., Simona Picchietti Phd, Francesco Buonocore Phd, Noelia Nuñez Ortiz, Valentina Stocchi, Laura Guerra Phd, Elisa Randelli Phd

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


Understanding Viral Dsrna Production, Cell Entry And Use As An Antiviral Therapeutic In Rainbow Trout, Sarah J. Poynter, Amal Aloufi, Jeremy Weleff, Adam Soares, Stephanie J. DeWitte-Orr 2016 University of Waterloo

Understanding Viral Dsrna Production, Cell Entry And Use As An Antiviral Therapeutic In Rainbow Trout, Sarah J. Poynter, Amal Aloufi, Jeremy Weleff, Adam Soares, Stephanie J. Dewitte-Orr

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


Vaccination Of Rainbow Trout Against Enteric Redmouth Disease (Erm) Induces An Early Balanced Expression Of Cytokines In The Spleen But A Heightened Expression Of Acute Phase Proteins And Antimicrobial Peptides In Both Spleen And Gills, Eakapol Wangkahart, Christopher J. Secombes, Tiehui Wang Dr 2016 University of Aberdeen

Vaccination Of Rainbow Trout Against Enteric Redmouth Disease (Erm) Induces An Early Balanced Expression Of Cytokines In The Spleen But A Heightened Expression Of Acute Phase Proteins And Antimicrobial Peptides In Both Spleen And Gills, Eakapol Wangkahart, Christopher J. Secombes, Tiehui Wang Dr

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


Leveraging The Plant Biotechnology Toolbox For Aquaculture: Production Of Protein Therapeutants For Promoting Fish Immune Health, Lana L. Elkins 2016 Arkansas State University - Main Campus

Leveraging The Plant Biotechnology Toolbox For Aquaculture: Production Of Protein Therapeutants For Promoting Fish Immune Health, Lana L. Elkins

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


Modelling The Polarization, Migration And Neuromast Deposition In The Zebrafish Posterior Lateral Line System, Hildur Knutsdottir 2016 University of British Columbia

Modelling The Polarization, Migration And Neuromast Deposition In The Zebrafish Posterior Lateral Line System, Hildur Knutsdottir

Biology and Medicine Through Mathematics Conference

No abstract provided.


Explicitly Separating Growth And Motility In A Glioblastoma Tumor Model, Tracy Stepien, Erica Rutter, Meng Fan, Yang Kuang 2016 Arizona State University

Explicitly Separating Growth And Motility In A Glioblastoma Tumor Model, Tracy Stepien, Erica Rutter, Meng Fan, Yang Kuang

Biology and Medicine Through Mathematics Conference

No abstract provided.


Force Generation And Contraction Of Random Actomyosin Bundles., Dietmar B. Oelz 2016 Courant Institute (NYU)

Force Generation And Contraction Of Random Actomyosin Bundles., Dietmar B. Oelz

Biology and Medicine Through Mathematics Conference

No abstract provided.


Modeling Tracheal Occlusion In The Embryonic Lung, Uduak George 2016 University of Louisiana Monroe

Modeling Tracheal Occlusion In The Embryonic Lung, Uduak George

Biology and Medicine Through Mathematics Conference

No abstract provided.


Exosomes Derived From Alcohol-Treated Hepatocytes Horizontally Transfer Liver Specific Mirna-122 And Sensitize Monocytes To Lps, Fatemeh Momen-Heravi, Shashi Bala, Karen Kodys, Gyongyi Szabo 2016 University of Massachusetts Medical School

Exosomes Derived From Alcohol-Treated Hepatocytes Horizontally Transfer Liver Specific Mirna-122 And Sensitize Monocytes To Lps, Fatemeh Momen-Heravi, Shashi Bala, Karen Kodys, Gyongyi Szabo

Gyongyi Szabo

Hepatocyte damage and inflammation in monocytes/macrophages are central to the pathogenesis of alcoholic hepatitis (AH). MicroRNAs (miRNAs) regulate all of these processes. MiRNA-122 is abundantly expressed in hepatocytes while monocytes/macrophages have low levels. The role of exosomes in AH and possible cross talk between hepatocyte-derived exosomes and immune cells is not explored yet. Here, we show that the number of exosomes significantly increases in the sera of healthy individuals after alcohol binge drinking and in mice after binge or chronic alcohol consumption. Exosomes isolated from sera after alcohol consumption or from in vitro ethanol-treated hepatocytes contained miRNA-122. Exosomes ...


Nuclear Accessibility Of Beta-Actin Mrna Is Measured By 3d Single-Molecule Real-Time Tracking, Carlas Smith, Stephan Preibisch, Aviva Joseph, Sara Abrahamsson, Bernd Rieger, Eugene Myers, Robert H. Singer, David Grünwald 2016 University of Massachusetts Medical School

Nuclear Accessibility Of Beta-Actin Mrna Is Measured By 3d Single-Molecule Real-Time Tracking, Carlas Smith, Stephan Preibisch, Aviva Joseph, Sara Abrahamsson, Bernd Rieger, Eugene Myers, Robert H. Singer, David Grünwald

David Grünwald

Imaging single proteins or RNAs allows direct visualization of the inner workings of the cell. Typically, three-dimensional (3D) images are acquired by sequentially capturing a series of 2D sections. The time required to step through the sample often impedes imaging of large numbers of rapidly moving molecules. Here we applied multifocus microscopy (MFM) to instantaneously capture 3D single-molecule real-time images in live cells, visualizing cell nuclei at 10 volumes per second. We developed image analysis techniques to analyze messenger RNA (mRNA) diffusion in the entire volume of the nucleus. Combining MFM with precise registration between fluorescently labeled mRNA, nuclear pore ...


Activation Kinetics Of Skinned Cardiac Muscle By Laser Photolysis Of Nitrophenyl-Egta, H. Martin, Marcus G. Bell, G. C. R. Ellis-Davies, Robert J. Barsotti 2016 Philadelphia College of Osteopathic Medicine

Activation Kinetics Of Skinned Cardiac Muscle By Laser Photolysis Of Nitrophenyl-Egta, H. Martin, Marcus G. Bell, G. C. R. Ellis-Davies, Robert J. Barsotti

Marcus G. Bell

The kinetics of Ca2+-induced contractions of chemically skinned guinea pig trabeculae was studied using laser photolysis of NP-EGTA. The amount of free Ca2+ released was altered by varying the output from a frequency-doubled ruby laser focused on the trabeculae, while maintaining constant total [NP-EGTA] and [Ca2+]. The time courses of the rise in stiffness and tension were biexponential at 23°C, Ph 7.1, and 200 Mm ionic strength. At full activation (pCa < 5.0), the rates of the rapid phase of the stiffness and tension rise were 56 ± 7 s-1 (n = 7) and 48 ± 6 s-1 ...


Effect Of Bisphenol-A On Neurodevelopment In Drosophila Melanogaster Larvae, Alexandra M. Streifel 2016 College of Saint Benedict/Saint John's University

Effect Of Bisphenol-A On Neurodevelopment In Drosophila Melanogaster Larvae, Alexandra M. Streifel

All College Thesis Program

This paper examines the effects of the chemical preservative bisphenol-A on the developing nervous systems of Drosophila melanogaster, or fruit fly, larvae. This study examines the effects of bisphenol-A using both behavioral as well as morphological paradigms. It was determined that bisphenol-A significantly increased the amount of time larvae spent in seeking behavior, the number of peristaltic contractions, and the distance traveled in a linear fashion. In the morphological analysis, indications of increased dendritic area in experimental larvae existed, but there was not enough data to determine significance. For future research, it is recommended that more data be gathered to ...


Engineering A Mutation In The Heparin Binding Pocket Of The Human Fibroblast Growth Factor, Roshni Patel 2016 University of Arkansas, Fayetteville

Engineering A Mutation In The Heparin Binding Pocket Of The Human Fibroblast Growth Factor, Roshni Patel

Chemistry & Biochemistry Undergraduate Honors Theses

Fibroblast growth factors (FGFs) are family of proteins that belong to a group of growth factors that are found in mammals and play an important role in angiogenesis, differentiation, organogenesis, and tissue repair. In summary, their main functionality is involved in cell division and proliferation. Because FGFs plays such a vital role in cell proliferation, they are mainly involved in the process of wound healing and injuries. FGF binds to its ligand, heparin—a heavily sulfated glycosaminoglycan. The binding of heparin to FGF occurs through electrostatic interactions, specifically between the negatively charged sulfate groups on heparin and positively charged residues ...


Genomic Drivers Of Cutaneous Squamous Cell Carcinoma Development, Vida Chitsazzadeh 2016 The University of Texas Graduate School of Biomedical Sciences at Houston

Genomic Drivers Of Cutaneous Squamous Cell Carcinoma Development, Vida Chitsazzadeh

UT GSBS Dissertations and Theses (Open Access)

Skin cancer is the most common malignancy in humans. Annually, in U.S. there are over 3 million cases with an estimated overall economic impact of $2 billion. Cutaneous Squamous Cell Carcinoma (cuSCC) comprises 15-20% of all skin cancers. cuSCC has the best-defined progression from a distinct precancerous lesion, the Actinic Keratosis (AK), to invasive cuSCC. Destructive therapies for AK treatment must be used repetitively, causing significant morbidity. There is a tremendous need for targeted diagnostics and therapy for AKs, representing an important opportunity for secondary skin cancer prevention. Our knowledge of the molecular and cellular events that lead to ...


Characterization Of Stem Cell Turnover In A Living Epithelial Bilayer, Elizabeth Sumner 2016 The University of Texas Graduate School of Biomedical Sciences at Houston

Characterization Of Stem Cell Turnover In A Living Epithelial Bilayer, Elizabeth Sumner

UT GSBS Dissertations and Theses (Open Access)

Homeostatic maintenance of epithelia requires the renewal and replacement of old or dying cells while sustaining a functional barrier. Imbalance between cell production and elimination are hypothesized to underlie many pathological conditions. However, our knowledge of cell turnover within living tissues remains largely restricted to static images due to the limited ability to study epithelia in their native context. Here we report that clearance of damaged basal stem cells promotes compensatory proliferation of neighboring stem cells to maintain overall population numbers in a bilayered epithelium. Time-lapse imaging and electron microscopy experiments reveal that dying cells are rapidly cleared as nearby ...


Exploring The Mechanisms Of Action Of Antifungal Peptides Using Saccharomyces Cerevisiae., Michelle L. Mason 2016 University of Arkansas, Fayetteville

Exploring The Mechanisms Of Action Of Antifungal Peptides Using Saccharomyces Cerevisiae., Michelle L. Mason

Biological Sciences Undergraduate Honors Theses

Candida albicans is a normal inhabitant of the skin and mucosal membranes of humans, however, in individuals with depressed immune systems or disrupted cutaneous flora, Candida can overgrow and cause serious infection. Candida infection is the fourth leading cause of nosocomial infection in the United States. These infections are often associated with longer hospital stays and higher mortality. Current drug therapies for this infection are largely ineffective due to the increased drug resistance of Candida species, and for some therapeutics, high levels of drug toxicity to humans. Histatin 5 is a naturally occurring salivary peptide that has strong antifungal properties ...


Staphylococcal Nuclease And Ubiquitin Local Folding Energies And Rates Using Peps-Hdx-Esi-Ms, Julie Rhee 2016 University of Arkansas, Fayetteville

Staphylococcal Nuclease And Ubiquitin Local Folding Energies And Rates Using Peps-Hdx-Esi-Ms, Julie Rhee

Chemistry & Biochemistry Undergraduate Honors Theses

In this study, Protein Equilibrium Population Snapshot Hydrogen-Deuterium Exchange Electrospray Ionization Mass Spectrometry (PEPS-HDX-ESI-MS) was applied to study the local regions of model proteins, staphylococcal nuclease and ubiquitin. The hydrogen deuterium exchange (HDX) has become a key technique for studying the structural and dynamic aspects of proteins in solution. This technique creates a rapid exchange between all of the exchangeable hydrogen ions with deuterium when the protein is exposed to a solvent. The PEPS method is an equilibrium-based method used to determine the populations of the closed native and open denatured states of a protein. By combining the applications of ...


Epigenetic Regulation Of Gene Expression During Spermatogenesis, Karishma Nayak 2016 The University of Rhode Island

Epigenetic Regulation Of Gene Expression During Spermatogenesis, Karishma Nayak

Senior Honors Projects

In the US livestock production industry, improving reproductive efficiency will improve animal welfare and maintain reasonable costs of meat and milk for consumers. In recent research, abnormalities in epigenetic markers in sperm during spermatogenesis, has been linked to male subfertility in many species. Epigenetics is the study of changes in organisms caused by modifications of gene expression, including DNA methylation, rather than alteration of the genetic code itself. When this process is disturbed, it can negatively impact semen therefore decreasing its fertility. Through further research on how DNA methylation influences gene expression during spermatogenesis and its impact on sperm quality ...


Role Of Chemokine Rantes In The Regulation Of Perivascular Inflammation, T-Cell Accumulation, And Vascular Dysfunction In Hypertension., Tomasz P Mikolajczyk, Ryszard Nosalski, Piotr Szczepaniak, Klaudia Budzyn, Grzegorz Osmenda, Paul J. Marvar, +10 additional authors 2016 George Washington University

Role Of Chemokine Rantes In The Regulation Of Perivascular Inflammation, T-Cell Accumulation, And Vascular Dysfunction In Hypertension., Tomasz P Mikolajczyk, Ryszard Nosalski, Piotr Szczepaniak, Klaudia Budzyn, Grzegorz Osmenda, Paul J. Marvar, +10 Additional Authors

Pharmacology and Physiology Faculty Publications

Recent studies have emphasized the role of perivascular inflammation in cardiovascular disease. We studied mechanisms of perivascular leukocyte infiltration in angiotensin II (Ang II)-induced hypertension and their links to vascular dysfunction. Chronic Ang II infusion in mice increased immune cell content of T cells (255 ± 130 to 1664 ± 349 cells/mg; P < 0.01), M1 and M2 macrophages, and dendritic cells in perivascular adipose tissue. In particular, the content of T lymphocytes bearing CC chemokine receptor (CCR) 1, CCR3, and CCR5 receptors for RANTES chemokine was increased by Ang II (CCR1, 15.6 ± 1.5% vs. 31 ± 5%; P < 0.01). Hypertension was associated with an increase in perivascular adipose tissue expression of the chemokine RANTES (relative quantification, 1.2 ± 0.2 vs. 3.5 ± 1.1; P < 0.05), which induced T-cell chemotaxis and vascular accumulation of T cells expressing the chemokine receptors CCR1, CCR3, and CCR5. Mechanistically, RANTES(-/-) knockout protected against vascular leukocyte, and in particular T lymphocyte infiltration (26 ± 5% in wild type Ang II vs. 15 ± 4% in RANTES(-/-)), which was associated with protection from endothelial dysfunction induced by Ang II. This effect was linked with diminished infiltration of IFN-γ-producing CD8(+) and double-negative CD3(+)CD4(-)CD8(-) T cells in perivascular space and reduced vascular oxidative stress while FoxP3(+) T-regulatory cells were unaltered. IFN-γ ex vivo caused significant endothelial dysfunction, which was reduced by superoxide anion scavenging. In a human cohort, a significant inverse correlation was observed between circulating RANTES levels as a biomarker and vascular function measured as flow-mediated dilatation (R = -0.3, P < 0.01) or endothelial injury marker von Willebrand factor (R = +0.3; P < 0.01). Thus, chemokine RANTES is important in the regulation of vascular dysfunction through modulation of perivascular inflammation.-Mikolajczyk, T. P., Nosalski, R., Szczepaniak, P., Budzyn, K., Osmenda, G., Skiba, D., Sagan, A., Wu, J., Vinh, A., Marvar, P. J., Guzik, B., Podolec, J., Drummond, G., Lob, H. E., Harrison, D. G., Guzik, T. J. Role of chemokine RANTES in the regulation of perivascular inflammation, T-cell accumulation, and vascular dysfunction in hypertension.


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