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4518 full-text articles. Page 1 of 165.

Superresolution Imaging Identifies That Conventional Trafficking Pathways Are Not Essential For Endoplasmic Reticulum To Outer Mitochondrial Membrane Protein Transport., Kyle Salka, Shivaprasad Bhuvanendran, Kassandra Wilson, Petros Bozidis, Mansi Mehta, Kristin Rainey, Hiromi Sesaki, George H Patterson, Jyoti K. Jaiswal, Anamaris M. Colberg-Poley 2017 George Washington University

Superresolution Imaging Identifies That Conventional Trafficking Pathways Are Not Essential For Endoplasmic Reticulum To Outer Mitochondrial Membrane Protein Transport., Kyle Salka, Shivaprasad Bhuvanendran, Kassandra Wilson, Petros Bozidis, Mansi Mehta, Kristin Rainey, Hiromi Sesaki, George H Patterson, Jyoti K. Jaiswal, Anamaris M. Colberg-Poley

Integrative Systems Biology Faculty Publications

Most nuclear-encoded mitochondrial proteins traffic from the cytosol to mitochondria. Some of these proteins localize at mitochondria-associated membranes (MAM), where mitochondria are closely apposed with the endoplasmic reticulum (ER). We have previously shown that the human cytomegalovirus signal-anchored protein known as viral mitochondria-localized inhibitor of apoptosis (vMIA) traffics from the ER to mitochondria and clusters at the outer mitochondrial membrane (OMM). Here, we have examined the host pathways by which vMIA traffics from the ER to mitochondria and clusters at the OMM. By disruption of phosphofurin acidic cluster sorting protein 2 (PACS-2), mitofusins (Mfn1/2), and dynamin related protein 1 ...


Hypericum In Infection: Identification Of Anti-Viral And Anti-Inflammatory Constituents, Diane F. Birt, Mark P. Widrlechner, Kimberly D. P. Hammer, Matthew L. Hillwig, Jingqiang Wei, George A. Kraus, Patricia A. Murphy, Joe-Ann McCoy, Eve S. Wurtele, Jeffrey D. Neighbors, David F. Wiemer, Wendy J. Maury, Jason P. Price 2017 Iowa State University

Hypericum In Infection: Identification Of Anti-Viral And Anti-Inflammatory Constituents, Diane F. Birt, Mark P. Widrlechner, Kimberly D. P. Hammer, Matthew L. Hillwig, Jingqiang Wei, George A. Kraus, Patricia A. Murphy, Joe-Ann Mccoy, Eve S. Wurtele, Jeffrey D. Neighbors, David F. Wiemer, Wendy J. Maury, Jason P. Price

Diane F. Birt


The Iowa Center for Research on Botanical Dietary Supplements seeks to optimize Echinacea, Hypericum, and Prunella botanical supplements for human-health benefit, emphasizing anti-viral, anti-inflammatory, and anti-pain activities. This mini-review reports on ongoing studies on Hypericum. The Center uses the genetically diverse, well-documented Hypericum populations collected and maintained at the USDA-ARS North Central Regional Plant Introduction Station (NCRPIS), and the strength of research in synthetic chemistry at Iowa State University to tap natural diversity, to help discover key constituents and interactions among constituents that impact bioactivity and toxicity. The NCRPIS has acquired more than 180 distinct populations of Hypericum, with a ...


Pseudohypericin Is Necessary For The Light-Activated Inhibition Of Prostaglandin E2 Pathways By A 4 Component System Mimicking An Hypericum Perforatum Fraction, Kimberly D. P. Hammer, Matthew L. Hillwig, Jeffrey D. Neighbors, Young-Je Sim, Marian L. Kohut, David F. Wiemer, Eve S. Wurtele, Diane F. Birt 2017 Iowa State University

Pseudohypericin Is Necessary For The Light-Activated Inhibition Of Prostaglandin E2 Pathways By A 4 Component System Mimicking An Hypericum Perforatum Fraction, Kimberly D. P. Hammer, Matthew L. Hillwig, Jeffrey D. Neighbors, Young-Je Sim, Marian L. Kohut, David F. Wiemer, Eve S. Wurtele, Diane F. Birt

Diane F. Birt

Hypericum perforatum (Hp) has been used medicinally to treat a variety of conditions including mild-to-moderate depression. Recently, several anti-inflammatory activities of Hp have been reported. An ethanol extract of Hp was fractionated with the guidance of an anti-inflammatory bioassay (lipopolysaccharide (LPS)-induced prostaglandin E2 production (PGE2)), and four constituents were identified. When combined together at concentrations detected in the Hp fraction to make a 4 component system, these constituents (0.1 μM chlorogenic acid, 0.08 μM amentoflavone, 0.07 μM quercetin, and 0.03 μM pseudohypericin) explained the majority of the activity of the fraction when activated by light ...


Metastatic Biomarkers In Synovial Sarcoma, Rosalia de Necochea-Campion, Lee M. Zuckerman, Hamid R. Mirshahidi, Shahrzad Khosrowpour, Chien-Shing Chen, Saied Mirshahidi 2017 Loma Linda University

Metastatic Biomarkers In Synovial Sarcoma, Rosalia De Necochea-Campion, Lee M. Zuckerman, Hamid R. Mirshahidi, Shahrzad Khosrowpour, Chien-Shing Chen, Saied Mirshahidi

Library Articles and Research

Synovial sarcoma (SS) is an aggressive soft tissue sarcoma (STS) that typically occurs in the extremities near a joint. Metastatic disease is common and usually occurs in the lungs and lymph nodes. Surgical management is the mainstay of treatment with chemotherapy and radiation typically used as adjuvant treatment. Although chemotherapy has a positive impact on survival, the prognosis is poor if metastatic disease occurs. The biology of sarcoma invasion and metastasis remain poorly understood. Chromosomal translocation with fusion of the SYT and SSX genes has been described and is currently used as a diagnostic marker, although the full impact of ...


Differentiation And Containment Of Derived Pancreatic Beta Cells, Caden Duffy, Alonzo Cook Ph.D. 2017 Brigham Young University - Provo

Differentiation And Containment Of Derived Pancreatic Beta Cells, Caden Duffy, Alonzo Cook Ph.D.

Biomedical Engineering Western Regional Conference

Currently, the only permanent cure for Type 1 Diabetes is a pancreatic or islet transplant. With the shortage of donors, we are progressing research towards alternative therapies by differentiating induced pluripotent stem cells into derived pancreatic β-cells that can be transplanted and used as treatment for Type 1 Diabetes. We are investigating methods to increase the yield of fully derived, insulin producing pancreatic β-cells and are additionally researching the use of hydrophilized expanded polytetrafluoroethylene for use in cell containment devices. These devices could offer a future alternative for islet transplantation in human patients.


Engineering Fret Biosensors For Microrna Presence/Absence Analysis, Nicholas E. Larkey, Sean M. Burrows 2017 Oregon State University

Engineering Fret Biosensors For Microrna Presence/Absence Analysis, Nicholas E. Larkey, Sean M. Burrows

Biomedical Engineering Western Regional Conference

No abstract provided.


Chimeric Antigen Receptor (Cars) For Thymidine Kinase 1 (Tk1): A Novel Immunotherapy Approach To Fight Cancer., Edwin Jesus Velazquez, Kiara Vaden, Michelle H. Townsend, Evita G. Weagel, Scott Weber, Richard A. Robison, Kim L. O'Neill 2017 Brigham Young University - Provo

Chimeric Antigen Receptor (Cars) For Thymidine Kinase 1 (Tk1): A Novel Immunotherapy Approach To Fight Cancer., Edwin Jesus Velazquez, Kiara Vaden, Michelle H. Townsend, Evita G. Weagel, Scott Weber, Richard A. Robison, Kim L. O'Neill

Biomedical Engineering Western Regional Conference

We have found the expression of Thymidine Kinase 1 (TK1) on the cell membrane of multiple cancer cell lines. We propose TK1 as a novel potential target for immune cell recognition in different cancer cell lines. Our research explores the use of these new targets in Chimeric antigen receptor (CAR) T-cell therapy.


Transcriptional And Post-Transcriptional Regulation Of Histone Variant H2a.Z During Sea Urchin Development, Mihai Hajdu 2017 The Graduate Center, City University of New York

Transcriptional And Post-Transcriptional Regulation Of Histone Variant H2a.Z During Sea Urchin Development, Mihai Hajdu

All Graduate Works by Year: Dissertations, Theses, and Capstone Projects

Histone variant H2A.Z promotes chromatin accessibility at transcriptional regulatory elements and is developmentally regulated in metazoans. We characterize the transcriptional and post-transcriptional regulation of H2A.Z in the purple sea urchin Strongylocentrotus purpuratus. H2A.Z depletion by antisense translation-blocking morpholino oligonucleotides during early development causes developmental collapse, in agreement with its previously demonstrated general role in transcriptional multipotency. During H2A.Z peak expression in 24-h embryos, endogenous H2A.Z 3’ UTR sequences stabilize GFP mRNAs relative to those with SV40 3’ UTR sequences, although the 3’UTR of H2A.Z does not determine the spatial distribution of H2A.Z ...


Analysis For Science Librarians Of The 2016 Nobel Prize In Physiology Or Medicine: The Life And Work Of Yoshinori Ohsumi, Neyda Gilman 2017 Binghamton University

Analysis For Science Librarians Of The 2016 Nobel Prize In Physiology Or Medicine: The Life And Work Of Yoshinori Ohsumi, Neyda Gilman

Library Scholarship

Autophagy is a cellular process of destruction and recycling. Cellular materials are broken down and recycled as needed, providing the body a way to remove unwanted material while also providing the means to create new needed items. The importance of this process and its possible role in numerous diseases is why Yoshinori Ohsumi has been awarded a 2016 Noble Prize. Ohsumi has been awarded the 2016 Nobel in Physiology or Medicine for his “discoveries of mechanisms for autophagy and thus paving the way for the exciting field of autophagy research” (Nobelprize.org “Press release: The 2016 Nobel Prize in Physiology ...


The Subject Librarian Newsletter, Biology, Spring 2017, Sandy Avila 2017 University of California, Irvine School of Law

The Subject Librarian Newsletter, Biology, Spring 2017, Sandy Avila

Libraries' Newsletters

No abstract provided.


Genistein Affects Her2 Protein Concentration, Activation And Promoter Regulation Via Estrogen Receptor-And Non-Estrogen Receptor-Mediated Mechanisms, Mary S. Sakla, Pete J. Ansell, Nader S. Shenouda, Dennis B. Lubahn, Ruth S. MacDonald 2017 University of Missouri

Genistein Affects Her2 Protein Concentration, Activation And Promoter Regulation Via Estrogen Receptor-And Non-Estrogen Receptor-Mediated Mechanisms, Mary S. Sakla, Pete J. Ansell, Nader S. Shenouda, Dennis B. Lubahn, Ruth S. Macdonald

Ruth S. MacDonald

The HER2 proto-oncogene, a member of the epidermal growth factor receptor family, is overexpressed in 20–30% of breast cancers. Genistein, the main soy isoflavone, interacts with estrogen receptors (ER) and it is also a potent tyrosine kinase inhibitor. Previously, our laboratory found that genistein delayed mammary tumor onset in transgenic mice that overexpress HER2 gene. Our goal was to define the mechanism through which genistein affects mammary tumorigenesis inHER2 overexpressing mice. We hypothesized that genistein inhibits HER2 activation and expression through ER-dependent and ER-independent mechanisms. Genistein inhibited total HER2 protein expression and tyrosine phosphorylation in BT-474, an ERα (−) and ...


Phytosterol Pygeum Africanum Regulates Prostate Cancer In Vitro And In Vivo, Nader S. Shenouda, Mary S. Sakla, Leslie G. Newton, Cynthia Besch-Williford, Norman M. Greenberg, Ruth S. MacDonald, Dennis B. Lubahn 2017 University of Missouri

Phytosterol Pygeum Africanum Regulates Prostate Cancer In Vitro And In Vivo, Nader S. Shenouda, Mary S. Sakla, Leslie G. Newton, Cynthia Besch-Williford, Norman M. Greenberg, Ruth S. Macdonald, Dennis B. Lubahn

Ruth S. MacDonald

Background

Prostate cancer is an important public health problem. It is an excellent candidate disease for chemo-prevention because prostate cancer is typically slow growing and is usually diagnosed in elderly males. Pygeum africanum (Prunus africana or Rosaceae) is an African prune (plum) tree found in tropical Africa. An extract from the bark of Pygeum africanum has been used in Europe as a prevention and treatment of prostate disorders including benign prostatic hypertrophy (BPH). More recently in the USA, the phytotherapeutic preparations of Pygeum africanum and Saw palmetto have been marketed for prostate health including prostate cancer prevention and treatment.

Methods ...


Soy Isoflavones Have An Antiestrogenic Effect And Alter Mammary Promoter Hypermethylation In Healthy Premenopausal Women1, Wenyi Qin, Weizhu Zhu, Huidong Shi, John E. Hewett, Rachel L. Ruhlen, Ruth S. MacDonald, George E. Rottinghaus, Yin-Chieh Chien, Edward R. Sauter 2017 University of Missouri School of Medicine

Soy Isoflavones Have An Antiestrogenic Effect And Alter Mammary Promoter Hypermethylation In Healthy Premenopausal Women1, Wenyi Qin, Weizhu Zhu, Huidong Shi, John E. Hewett, Rachel L. Ruhlen, Ruth S. Macdonald, George E. Rottinghaus, Yin-Chieh Chien, Edward R. Sauter

Ruth S. MacDonald

We hypothesized that soy isoflavones would have dose related estrogenic and methylation effects. 34 healthy premenopausal women were prospectively enrolled and randomized in double-blind fashion to receive either 40 mg or 140 mg isoflavones daily through one menstrual cycle. Breast specific (NAF) and systemic (serum) estrogenic effects were assessed measuring the estrogenic marker complement (C)3 and changes in cytology, while methylation effects were evaluated in mammary ductoscopy (MD) specimens using methylation specific PCR assessment of five genes (p16, RASSF1A, RARβ2, ER, and CCND2) associated with breast carcinogenesis. Serum genistein significantly increased post treatment in women consuming both isoflavone doses ...


Chloroquine-Inducible Par-4 Secretion Is Essential For Tumor Cell Apoptosis And Inhibition Of Metastasis, Ravshan Burikhanov, Nikhil Hebbar, Sunil K. Noothi, Nidhi Shukla, James Sledziona, Nathália Araujo, Meghana Kudrimoti, Qing Jun Wang, David S. Watt, Danny R. Welch, Jodi Maranchie, Akihiro Harada, Vivek M. Rangnekar 2017 University of Kentucky

Chloroquine-Inducible Par-4 Secretion Is Essential For Tumor Cell Apoptosis And Inhibition Of Metastasis, Ravshan Burikhanov, Nikhil Hebbar, Sunil K. Noothi, Nidhi Shukla, James Sledziona, Nathália Araujo, Meghana Kudrimoti, Qing Jun Wang, David S. Watt, Danny R. Welch, Jodi Maranchie, Akihiro Harada, Vivek M. Rangnekar

Radiation Medicine Faculty Publications

The induction of tumor suppressor proteins capable of cancer cell apoptosis represents an attractive option for the re-purposing of existing drugs. We report that the anti-malarial drug, chloroquine (CQ), is a robust inducer of Par-4 secretion from normal cells in mice and cancer patients in a clinical trial. CQ-inducible Par-4 secretion triggers paracrine apoptosis of cancer cells and also inhibits metastatic tumor growth. CQ induces Par-4 secretion via the classical secretory pathway that requires the activation of p53. Mechanistically, p53 directly induces Rab8b, a GTPase essential for vesicle transport of Par-4 to the plasma membrane prior to secretion. Our findings ...


Caf-1 P150 And Ki-67 Regulate Nuclear Structure Throughout The Human Cell Cycle, Timothy D. Matheson 2017 University of Massachusetts Medical School

Caf-1 P150 And Ki-67 Regulate Nuclear Structure Throughout The Human Cell Cycle, Timothy D. Matheson

GSBS Dissertations and Theses

The three-dimensional organization of the human genome is non-random in interphase cells. Heterochromatin is highly clustered at the nuclear periphery, adjacent to nucleoli, and near centromeres. These localizations are reshuffled during mitosis when the chromosomes are condensed, nucleoli disassembled, and the nuclear envelope broken down. After cytokinesis, heterochromatin is re-localized to the domains described above. However, the mechanisms by which this localization is coordinated are not well understood. This dissertation will present evidence showing that both CAF-1 p150 and Ki-67 regulate nuclear structure throughout the human cell cycle.

Chromatin Assembly Factor 1 (CAF-1) is a highly conserved three-subunit protein complex ...


Modulation Of Pro-Inflammatory Cytokines Expression Of The Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation In Mouse Splenocytes, Susan Starling 2017 Trivedi Global Inc

Modulation Of Pro-Inflammatory Cytokines Expression Of The Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation In Mouse Splenocytes, Susan Starling

Susan Elizabeth Starling

With the increasing popularity of herbomineral preparations in healthcare, a new proprietary herbomineral formulation was formulated with ashwagandha root extract and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation using murine splenocyte cells. The test formulation was divided into two parts. One was the control without the Biofield Energy Treatment. The other part was labelled the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers. Through MTT assay, all the ...


Modulation Of Pro-Inflammatory Cytokines Expression Of The Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation In Mouse Splenocytes, Peter Surguy 2017 Trivedi Global Inc

Modulation Of Pro-Inflammatory Cytokines Expression Of The Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation In Mouse Splenocytes, Peter Surguy

Peter Lynwood Surguy

With the increasing popularity of herbomineral preparations in healthcare, a new proprietary herbomineral formulation was formulated with ashwagandha root extract and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation using murine splenocyte cells. The test formulation was divided into two parts. One was the control without the Biofield Energy Treatment. The other part was labelled the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers. Through MTT assay, all the ...


Modulation Of Pro-Inflammatory Cytokines Expression Of The Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation In Mouse Splenocytes, Susan Allen 2017 Trivedi Global Inc.

Modulation Of Pro-Inflammatory Cytokines Expression Of The Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation In Mouse Splenocytes, Susan Allen

Susan Mardis Allen

With the increasing popularity of herbomineral preparations in healthcare, a new proprietary herbomineral formulation was formulated with ashwagandha root extract and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation using murine splenocyte cells. The test formulation was divided into two parts. One was the control without the Biofield Energy Treatment. The other part was labelled the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers. Through MTT assay, all the ...


Modulation Of Pro-Inflammatory Cytokines Expression Of The Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation In Mouse Splenocytes, Sara Friedman 2017 Selected Works

Modulation Of Pro-Inflammatory Cytokines Expression Of The Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation In Mouse Splenocytes, Sara Friedman

Sara Friedman

With the increasing popularity of herbomineral preparations in healthcare, a new proprietary herbomineral formulation was formulated with ashwagandha root extract and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation using murine splenocyte cells. The test formulation was divided into two parts. One was the control without the Biofield Energy Treatment. The other part was labelled the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers. Through MTT assay, all the ...


Modulation Of Pro-Inflammatory Cytokines Expression Of The Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation In Mouse Splenocytes, Steven Christie 2017 Selected Works

Modulation Of Pro-Inflammatory Cytokines Expression Of The Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation In Mouse Splenocytes, Steven Christie

Steven Lee Christie

With the increasing popularity of herbomineral preparations in healthcare, a new proprietary herbomineral formulation was formulated with ashwagandha root extract and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation using murine splenocyte cells. The test formulation was divided into two parts. One was the control without the Biofield Energy Treatment. The other part was labelled the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers. Through MTT assay, all the ...


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