The Roles Of Hedgehog And Ap-2 Signaling In The Regulation Of Lens Development, 2014 McMaster University
The Roles Of Hedgehog And Ap-2 Signaling In The Regulation Of Lens Development, Christine L. Kerr
Open Access Dissertations and Theses
Lens development is an intricate process governed by growth factor signaling and a hierarchy of transcription factors that regulate important processes required for normal lens development.
Midline hedgehog (Hh) signaling has been implicated in lens defects including cyclopia and lens degeneration in rodents and fish. A lens specific model of hedgehog signaling has not been examined, and it was unknown whether the lens is able to respond to Hh signals. To investigate this question, and to determine any consequences of abnormal Hh signaling on lens development, a mouse model of constitutively active smoothened in the surface ectoderm and derivatives, (including ...
3b Project Data - Raw Tiff Series And Cropped Series, 2014 The University of Texas
3b Project Data - Raw Tiff Series And Cropped Series, George Mcnamara
Fluorescence microscopy data acquired for 3B Bayesian localization microscopy.
Settings: 100 nm XY pixel size. 50 millisecond exposures, MetaMorph stream acquisition mode (as fast as possible). Stellaris FISH mammalian cells labeled with Quasar 670 fluorophores, one molecule per 20mer FISH probe, ~48 probes to TOP1 mRNA (Topoisomerase 1).
More on the 3B project and Stellaris FISH data at http://works.bepress.com/gmcnamara/38/
and at Susan Cox's web sites http://www.coxphysics.com/3b/ http://superresolved.com/
Smurf2 Regulates Hematopoietic Stem Cell Self-Renewal And Aging, 2014 University of Massachusetts Medical School
Smurf2 Regulates Hematopoietic Stem Cell Self-Renewal And Aging, Charusheila Ramkumar, Yahui Kong, Sally E. Trabucco, Rachel M. Gerstein, Hong Zhang
GSBS Student Publications
The age-dependent decline in the self-renewal capacity of stem cells plays a critical role in aging, but the precise mechanisms underlying this decline are not well understood. By limiting proliferative capacity, senescence is thought to play an important role in age-dependent decline of stem cell self-renewal, although direct evidence supporting this hypothesis is largely lacking. We have previously identified the E3 ubiquitin ligase Smurf2 as a critical regulator of senescence. In this study, we found that mice deficient in Smurf2 had an expanded hematopoietic stem cell (HSC) compartment in bone marrow under normal homeostatic conditions, and this expansion was associated ...
Endocytosis And Vacuolar Degradation Of The Yeast Cell Surface Glucose Sensors Rgt2 And Snf3, 2014 Himmelfarb Health Sciences Library, The George Washington University
Endocytosis And Vacuolar Degradation Of The Yeast Cell Surface Glucose Sensors Rgt2 And Snf3, Adhiraj Roy, Jeong-Ho Kim
Biochemistry and Molecular Medicine Faculty Publications
Sensing and signaling the presence of extracellular glucose is crucial for the yeast Saccharomyces cerevisiae because of its fermentative metabolism, characterized by high glucose flux through glycolysis. The yeast senses glucose through the cell surface glucose sensors Rgt2 and Snf3, which serve as glucose receptors that generate the signal for induction of genes involved in glucose uptake and metabolism. Rgt2 and Snf3 detect high and low glucose concentrations, respectively, perhaps due to their different affinities for glucose. Here, we provide evidence that cell surface levels of glucose sensors are regulated by ubiquitination and degradation. The glucose sensors are removed from ...
Blockade Of Mast Cell Activation Reduces Cutaneous Scar Formation, 2014 Illinois Mathematics and Science Academy
Blockade Of Mast Cell Activation Reduces Cutaneous Scar Formation, Lin Chen, Megan Schrementi, Matthew J. Ranzer, Traci A. Wilgus, Luisa A. Dipietro
Faculty Publications & Research
Damage to the skin initiates a cascade of well-orchestrated events that ultimately leads to repair of the wound. The inflammatory response is key to wound healing both through preventing infection and stimulating proliferation and remodeling of the skin. Mast cells within the tissue are one of the first immune cells to respond to trauma, and upon activation they release pro-inflammatory molecules to initiate recruitment of leukocytes and promote a vascular response in the tissue. Additionally, mast cells stimulate collagen synthesis by dermal fibroblasts, suggesting they may also influence scar formation. To examine the contribution of mast cells in tissue repair ...
Transcription Factor Binding To Caenorhabditis Elegans First Introns Reveals Lack Of Redundancy With Gene Promoters, 2014 University of Massachusetts Medical School
Transcription Factor Binding To Caenorhabditis Elegans First Introns Reveals Lack Of Redundancy With Gene Promoters, Juan I. Fuxman Bass, Alex M. Tamburino, Akihiro Mori, Nathan Beittel, Matthew T. Weirauch, John S. Reece-Hoyes, Albertha J. M. Walhout
Program in Systems Biology Publications and Presentations
Gene expression is controlled through the binding of transcription factors (TFs) to regulatory genomic regions. First introns are longer than other introns in multiple eukaryotic species and are under selective constraint. Here we explore the importance of first introns in TF binding in the nematode Caenorhabditis elegans by combining computational predictions and experimentally derived TF-DNA interaction data. We found that first introns of C. elegans genes, particularly those for families enriched in long first introns, are more conserved in length, have more conserved predicted TF interactions and are bound by more TFs than other introns. We detected a significant positive ...
Blood Vessel Z-Series Image Data Set From Confocal Microscopy 2013 Book, 2014 The University of Texas
Blood Vessel Z-Series Image Data Set From Confocal Microscopy 2013 Book, George Mcnamara
Blood Vessel Z-series image data set from Confocal Microscopy 2013 book chapter:
McNamara G, Yanai A, Khankaldyyan V, Laug WE, Boden J, Webster K, Li Y, Wen R. Low magnification confocal microscopy of tumor angiogenesis. Methods Mol Biol. 2014; 1075: 149-75.
doi: 10.1007/978-1-60761-847-8_6. PubMed PMID: 24052350.
Jeffrey Boden, Jianqin Wei, George McNamara, Hans Layman, Midhat Abdulreda, Fotios Andreopoulos, and Keith A. Webster. Whole-mount imaging of the mouse hindlimb vasculature using the lipophilic carbocyanine dye DiI. Biotechniques Rapid Dispatches, July 2012, pp. 1–4
Targeting Cxcr4 With Ctce-9908 Inhibits Prostate Tumor Metastasis, 2014 Wayne State University
Targeting Cxcr4 With Ctce-9908 Inhibits Prostate Tumor Metastasis, Donald Wong, Pridvi Kandagatla, Walter Korz, Sreenivasa R. Chinni
Wayne State University Associated BioMed Central Scholarship
CXCL12/CXCR4 transactivation of epidermal growth factor family receptors in lipid raft membrane microdomains on cell surface is thought to mediate tumor growth and subsequent development of metastatic disease. CTCE-9908 is a known inhibitor of CXCR4. Herein, we tested the efficacy of CTCE-9908 in inhibiting prostate cancer cell growth, invasion, and metastasis.
We used a panel ofin vitroassays utilizing human prostate cancer cell lines and anin vivoorthotopic prostate cancer model to assess the anti-tumoral activity of CTCE-9908.
We demonstrated that (a) CTCE-9908 treatment resulted in no significant change in the growth of PC-3 and C4-2B cells ...
Recombinant Interleukin-21 Plus Sorafenib For Metastatic Renal Cell Carcinoma: A Phase 1/2 Study, 2014 Wayne State University
Recombinant Interleukin-21 Plus Sorafenib For Metastatic Renal Cell Carcinoma: A Phase 1/2 Study, Shailender Bhatia, Brendan, Marc S. Ernstoff, Michael, Elisabeth I. Heath, Wilson H. Miller Jr, Igor Puzanov, David I. Quinn, Thomas, Peter Vanveldhuizen, Kelly, Jeremy, Rachel, Naomi, Sonia, John A. Thompson
Wayne State University Associated BioMed Central Scholarship
Despite the positive impact of targeted therapies on metastatic renal cell carcinoma (mRCC), durable responses are infrequent and an unmet need exists for novel therapies with distinct mechanisms of action. We investigated the combination of recombinant Interleukin 21 (IL-21), a cytokine with unique immunostimulatory properties, plus sorafenib, a VEGFR tyrosine kinase inhibitor.
In this phase 1/2 study, 52 mRCC patients received outpatient treatment with oral sorafenib 400 mg twice daily plus intravenous IL-21 (10–50 mcg/kg) on days 1–5 and 15–19 of each 7-week treatment course. The safety, antitumor activity, pharmacokinetic and pharmacodynamic ...
Calpain 5: A Non-Classical Calpain Highly Expressed In The Cns And Localized To Mitochondria And Nuclear Pml Bodies, 2014 University of Kentucky
Calpain 5: A Non-Classical Calpain Highly Expressed In The Cns And Localized To Mitochondria And Nuclear Pml Bodies, Ranjana Singh
Theses and Dissertations--Anatomy and Neurobiology
Calpain 5 (CAPN5) is a non-classical member of the calpain family. It lacks the EF-hand motif characteristic of the classical calpains, calpain 1 and 2, but retains catalytic and Ca2+ binding non EF domains. Tra-3, an ortholog of CAPN5, is involved in necrotic cell death in C.elegans; although specific role of CAPN5 has not been investigated in the mammalian CNS. I compared relative mRNA levels of calpains in rat CNS, which revealed that CAPN5 is the second most highly expressed calpain. We examined relative levels of CAPN5 from late embryonic day 18 to postnatal day 90 and found ...
Partitioning The Fitness Components Of Rna Populations Evolving In Vitro, 2013 Portland State University
Partitioning The Fitness Components Of Rna Populations Evolving In Vitro, Carolina Diaz Arenas, Niles Lehman
Chemistry Faculty Publications and Presentations
All individuals in an evolving population compete for resources, and their performance is measured by a fitness metric. The performance of the individuals is relative to their abilities and to the biotic surroundings – the conditions under which they are competing – and involves many components. Molecules evolving in a test tube can also face complex environments and dynamics, and their fitnessmeasurements should reflect the complexity of various contributing factors as well. Here, the fitnesses of a set of ligase ribozymes evolved by the continuous in vitroevolution system were measured. During these evolution cycles there are three different catalytic steps, ligation ...
Stellaris Fish Workshop Gadph And Dapi Z-Series, 2013 The University of Texas
Stellaris Fish Workshop Gadph And Dapi Z-Series, George Mcnamara
Stellaris FISH workshop GADPH and DAPI Z-series at MD Anderson Cancer Center, Houston, TX.
The zip file contains raw and GPU deconvolved image data from a workshop Biosearch Technologies conducted for MDACC researchers in December 2013. Image data was acquired on a Leica DMI6000 microscope with Lumencor SOLA light engine, DAPI and Cy5 filter cubes, Hamamatsu ORCA FLASH4.0 sCMOS camera (500 ms exposure time per plane for Quasar 670).
Pixel size 100 nm XY.
Z-step size 200 nm.
32 planes (power of 2 is optimal for GPU deconvolution). With 500 ms exposure time, the Quasar 670 GADPH FISH probes ...
Genetic Sex Conditions And Redefining Sex, 2013 Wofford College
Genetic Sex Conditions And Redefining Sex, Jayce O'Shields
Western culture has a tendency to value binaries and discreet categories that separate its social structure and provide a sense of order and organization. The value placed on binaries and categories may be advantageous in some aspects, but when it starts to infringe upon the legal and medical rights of individuals not easily placed in either binary category, it can become less advantageous.
A baby is usually classified as either male or female shortly after birth, and all future legal, social, and economic actions and rights of that individual are more or less decided according to this classification. A problem ...
Prenatal Programming Of Hepatic Glucose And Cholesterol Regulation In Male Rat Offspring By Chronic Intermittent Hypoxia, Waseem Iqbal
University of Western Ontario - Electronic Thesis and Dissertation Repository
Obstructive sleep apnea (OSA) is a chronic disorder involving repetitive interruptions in breathing during sleep. Sufferers of OSA are exposed to chronic intermittent hypoxia (CIH), characterized by cyclical reductions in oxygen availability. A number of studies have established a link between OSA and various cardiovascular and metabolic comorbidities in adulthood, including hypertension, obesity, and type II diabetes. While the consequences of OSA in adults have been well described, the cross-generational impact of this condition and potential effects on fetal development are not known. Epidemiological and animal studies have demonstrated that physiological insults during pregnancy lead to diminished growth of offspring ...
From Heart Beats To Health Recipes: The Role Of Fractal Physiology In The Ancestral Health Movement, 2013 Ancestral Health Society
From Heart Beats To Health Recipes: The Role Of Fractal Physiology In The Ancestral Health Movement, Aaron P. Blaisdell, Brent Pottenger, John S. Torday
Journal of Evolution and Health
The human body—an amazing biological system that scales up fractally from its cellular building blocks—exhibits an incredible ability to self heal. Why then, are chronic diseases and degeneration on the rise in the population? Why are we sicker, more obese, and more depressed and stressed than ever before in human history? Why can’t we heal? The answers to these questions may lie in our ancestry, and modern departure from the human ecological niche. The ability to heal requires proper spatio-temporal inputs—nutrition, sleep, stress, activity, and socialization—in order for cellular signaling to occur properly across semi-permeable ...
Protective Effects Of Sphingomyelin Against Uv Photodamage In Human Keratinocytes, 2013 California Polytechnic State University
Protective Effects Of Sphingomyelin Against Uv Photodamage In Human Keratinocytes, Kathleen De Guzman
Master's Theses and Project Reports
Ultraviolet (UV) radiation has been demonstrated in numerous studies to be a major risk factor for non-melanoma skin cancer development. Despite the emergence of current UV-preventative strategies, such as sunscreens and skin-protective clothing, the incidence of non-melanoma skin cancer has continued to rise. This has encouraged investigations on alternative methods for UV prevention. In particular, bovine milk sphingomyelin has been studied for its potential in protecting human skin against UV photodamage. While the previous studies have suggested that sphingomyelin exhibits UV-protective properties in a human skin equivalent model, the exact mechanisms behind sphingomyelin’s photoprotective effects are yet unknown.
C-Jun N-Terminal Kinases Regulate Adenovirus-Mediated Autophagy And Antigen Presentation, 2013 Texas Medical Center Library
C-Jun N-Terminal Kinases Regulate Adenovirus-Mediated Autophagy And Antigen Presentation, Sarah R. Klein
UT GSBS Dissertations and Theses (Open Access)
Targeted immunotherapy with recombinant, oncolytic adenoviruses is under investigation for the treatment of cancer. Evidence indicates adenoviruses induce autophagy that is required for oncolysis, but the molecular regulation of autophagy in infected cells remains under investigation. Our data suggested the canonical pathway regulating starvation-induced autophagy was not implemented in adenovirus-induced autophagy; however, adenovirus infection triggered phosphorylation of c-Jun N-terminal kinases (JNK) that was essential for autophagy. Adenoviral replication within the host cell elicited JNK pathway activation leading to B cell lymphoma-2 (Bcl-2) phosphorylation. JNK-dependent Bcl-2 phosphorylation stimulated the dissociation of Bcl-2/beclin 1 heterodimers, enabling beclin 1 to initiate autophagy ...
Investigating Apoptosis Pathway In Chronic Lymphocytic Leukemia: Stromal Influence And Therapeutic Activation, 2013 Texas Medical Center Library
Investigating Apoptosis Pathway In Chronic Lymphocytic Leukemia: Stromal Influence And Therapeutic Activation, Viralkumar M. Patel
UT GSBS Dissertations and Theses (Open Access)
Chronic lymphocytic leukemia (CLL) is a B-cell malignancy. High levels of Bcl-2 and IAP family proteins are responsible for apoptotic-resistance and accumulation of mature CLL lymphocytes in bone-marrow, lymph nodes and peripheral blood. Besides pro-survival proteins, supporting stromal cells as well as soluble factors in the microenvironment of bone-marrow and lymph nodes provide survival advantage to CLL leukemic cells.
Though the stromal – leukemia cell interactions has been studied extensively, in-depth-knowledge on the regulation of apoptotic pathway proteins in the context of microenvironment is still limited. To address this, the first part of our study focused on comprehensive analysis of 93 ...
Developmental And Molecular Functions Of Plakophilin-3, 2013 Texas Medical Center Library
Developmental And Molecular Functions Of Plakophilin-3, William A. Munoz
UT GSBS Dissertations and Theses (Open Access)
Plakophilin-3, the less studied member of the plakophilin-catenin subfamily, and the larger catenin family, binds directly to desmosomal cadherin cytoplasmic domains and enhances desmosome formation and stability. In mammals, plakophilin-3 is expressed at the highest levels in desmosome-enriched tissues such as epithelia, with the knock-out in mice producing corresponding reductions in ectodermal integrity. In tissue, cellular and intracellular contexts where plakophilin-3 is not at the desmosomal plaque, little is known about its functions in the cytoplasm or nucleus, where it also localizes.
My work employed embryos of the amphibian, Xenopus laevis, to examine plakophilin-3’s developmental roles. I first evaluated ...
Functional Overlap Among Distinct G1/S Inhibitory Pathways Allows Robust G1 Arrest By Yeast Mating Pheromones, 2013 University of Massachusetts Medical School
Functional Overlap Among Distinct G1/S Inhibitory Pathways Allows Robust G1 Arrest By Yeast Mating Pheromones, Patricia A. Pope, Peter M. Pryciak
GSBS Student Publications
In budding yeast, mating pheromones arrest the cell cycle in G1 phase via a pheromone-activated Cdk-inhibitor (CKI) protein, Far1. Alternate pathways must also exist, however, because deleting the cyclin CLN2 restores pheromone arrest to far1 cells. Here we probe whether these alternate pathways require the G1/S transcriptional repressors Whi5 and Stb1 or the CKI protein Sic1, whose metazoan analogues (Rb or p27) antagonize cell cycle entry. Removing Whi5 and Stb1 allows partial escape from G1 arrest in far1 cln2 cells, along with partial derepression of G1/S genes, which implies a repressor-independent route for inhibiting G1/S transcription. This ...