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Tattletales And T-Bow Update 20141018, George McNamara 2014 The University of Texas

Tattletales And T-Bow Update 20141018, George Mcnamara

George McNamara

Tattletales and T-Bow Update 20141018

http://works.bepress.com/gmcnamara/63

Tattletales is my concept to multiplex fluorescent protein biosensors ("Tattletales", which is also the overall concept name) and multicolor FP reporters ("T-Bow" for Rainbow T-cells and tumor cells) to study the physiology of live cells.

These are based on:

1. LacI-GFP :: LacO operator synthetic tandem repeat array (Robinett et al 1996 JCB, Fig 4A, http://jcb.rupress.org/content/135/6/1685.full.pdf )

2. the plethora of fluorescent protein biosensors - see page 1 of this "63" download site.

3. the many colors, and combinations of colors (CY11.5 ...


Ligand-Directed Signaling And Trafficking At The Nociceptin Opioid Peptide Receptor, Gina E. Migneco 2014 Washington University in St. Louis

Ligand-Directed Signaling And Trafficking At The Nociceptin Opioid Peptide Receptor, Gina E. Migneco

Undergraduate Research Symposium Posters & Abstracts

Traditional opioid receptors such as the μ and κ opioid receptors (MOR and

KOR) are well studied and are found to be common targets for pain and

stress therapies. The nociceptin opioid receptor (NOPR/ORL1) is a most

recently discovered opioid receptor whose complete function is still relatively

unknown, however is of great interest to researchers due to its analgesic

and antidepressant properties. NOPR activation due to ligand binding

triggers a variety of downstream effects, including G-protein signaling,

arrestin-mediated signaling, and receptor internalization. A variety of NOPR

ligands, both putative agonists and antagonists, were characterized by

further examination of these ...


Gpu Deconvolution Good Result On 2048x2048x32 Plane Z-Series (Update From Dataset 56), George McNamara 2014 The University of Texas

Gpu Deconvolution Good Result On 2048x2048x32 Plane Z-Series (Update From Dataset 56), George Mcnamara

George McNamara

GPU Deconvolution Good result on 2048x2048x32 plane Z-series (update from dataset 56)

This web page and download is

http://works.bepress.com/gmcnamara/62

Marc Bruce sent me the file here (web page 62) that is a preliminary bug fix (apparently of NVidia CUDA FFT code!!!) from the original 'raw' Z-series that is part of the

http://works.bepress.com/gmcnamara/56

web page download. Please see that web page for details on the project (GADPH single molecule mRNA detection with Stellaris FISH probe sets).

Marc is:

MARC BRUCE, PHD

CTO and Co-Founder

MICROVOLUTION, LLC

http://www.microvolution.com/

//

The ...


Electrophoresis Staining: A New Method Of Whole Mount Staining, Mitchell G. Ayers, Sarah Calve, Zhiyu Li 2014 Purdue University

Electrophoresis Staining: A New Method Of Whole Mount Staining, Mitchell G. Ayers, Sarah Calve, Zhiyu Li

The Summer Undergraduate Research Fellowship (SURF) Symposium

Advances in tissue clearing techniques have allowed almost a ten-fold increase in the viewing depth of confocal microscopy. This allows for intact cellular structures to be rendered in 3D. However, viewing tissues to this depth is often limited to endogenous fluorescence as passive diffusion of antibodies via whole mount staining can take weeks. Our lab is developing a new method involving electrophoresis as a driving force that will promote active antibody binding deep into tissue, reducing the amount of time needed to stain for cellular structures. Due to the inherent charge within antibodies, they are able to be directionally forced ...


Developing An Induced Pluripotent Stem Cell Model Of Lowe Syndrome, Kyle Patton, Wen-Chieh Hsieh, R. Claudio Aguilar, Donna Fekete 2014 Purdue University

Developing An Induced Pluripotent Stem Cell Model Of Lowe Syndrome, Kyle Patton, Wen-Chieh Hsieh, R. Claudio Aguilar, Donna Fekete

The Summer Undergraduate Research Fellowship (SURF) Symposium

Lowe syndrome (LS) is a fatal genetic disorder in which inheritance of a mutated form of the OCRL1 gene leads to kidney, eye, and brain defects. Understanding how LS impairs the function of these organs at the cellular level is important to gain a fuller perspective of the disease and enable the discovery of targets for treatment. Studies have revealed that LS fibroblasts have impaired abilities to migrate and generate sensory organelles known as primary cilia. However, it is unknown how these problems play out in other cell types from organs associated with the disease. In order to understand how ...


Spermitin: A Novel Mitochondrial Protein In Drosophila Spermatids, Jieyan Chen, Timothy L. Megraw 2014 Florida State University

Spermitin: A Novel Mitochondrial Protein In Drosophila Spermatids, Jieyan Chen, Timothy L. Megraw

Department of Biomedical Sciences Faculty Publications

Mitochondria, important energy centers in the cell, also control sperm cell morphogenesis.Drosophila spermatids have a remarkably large mitochondrial formation called the nebenkern. Immediately following meiosis during sperm development, the mitochondria in the spermatid fuse together into two large aggregates which then wrap around one another to produce the spherical nebenkern: a giant mitochondrion about 6 micrometers in diameter. The fused mitochondria play an important role in sperm tail elongation by providing a structural platform to support the elongation of sperm cells. We have identified a novel testis-specific protein, Spermitin (Sprn), a protein with a Pleckstrin homology-like (PH) domain related ...


Introduction To Cancer Concepts: A Guidebook For The Non-Oncologist, Richard S. Pieters, Maryann Bishop-Jodoin, James Liebmann 2014 University of Massachusetts Medical School

Introduction To Cancer Concepts: A Guidebook For The Non-Oncologist, Richard S. Pieters, Maryann Bishop-Jodoin, James Liebmann

Cancer Concepts: A Guidebook for the Non-Oncologist

This chapter in Cancer Concepts: A Guidebook for the Non-Oncologist presents an introduction to the Guidebook, which developed from the Cancer Concepts course at the University of Massachusetts Medical School.


The Effect Of Pomegranate Juice Extract On The Hedgehog Signaling Pathway In Pancreatic Cancer, Veronica Gomez, Talia Shackelford, Autumn Tocchi, Melissa Rowland-Goldsmith, Ph.D. 2014 Chapman University

The Effect Of Pomegranate Juice Extract On The Hedgehog Signaling Pathway In Pancreatic Cancer, Veronica Gomez, Talia Shackelford, Autumn Tocchi, Melissa Rowland-Goldsmith, Ph.D.

e-Research: A Journal of Undergraduate Work

Pancreatic cancer is the fourth leading cause of cancer death in the United States. There have been several reports indicating that phytochemicals in fruits can reduce the risk of cancer due to the anti-oxidant and anti-inflammatory effects of the polyphenols. Our lab has shown that pomegranate juice extract (PJE) has anti-proliferative and pro-apoptotic effects in human pancreatic cancer cells. In the past, we have shown that cells adhere more strongly to the plate when treated with PJE. This observation prompted an investigation of how PJE regulates cell adhesion proteins. Previously, our lab investigated E-cadherin, a cell adhesion protein. Upon activation ...


Environmental And Infectious Causes Of Malignancy, Mary Linton Peters, Richard S. Pieters, James Liebmann 2014 University of Massachusetts Medical School

Environmental And Infectious Causes Of Malignancy, Mary Linton Peters, Richard S. Pieters, James Liebmann

Cancer Concepts: A Guidebook for the Non-Oncologist

This chapter in Cancer Concepts: A Guidebook for the Non-Oncologist presents a summary of the most relevant causative agents of cancer. Exposure to many environmental agents is associated with an increased incidence of certain malignancies, although causation is usually difficult to prove. Certain chemicals, infections (parasitic, viral, and bacterial) and ionizing radiation are known carcinogens. Variable genetic susceptibility to carcinogenesis is apparent. Up to 2/3 of human cancers are believed to have an environmental component.


Poly(Arginine) Derived Cancer-Targeting Peptides For The Development Of A Cancer-Targeted Gene Therapy Approach In Hepg2 Liver Cancer Cells, Stesha C. Joseph 2014 Seton Hall University

Poly(Arginine) Derived Cancer-Targeting Peptides For The Development Of A Cancer-Targeted Gene Therapy Approach In Hepg2 Liver Cancer Cells, Stesha C. Joseph

Seton Hall University Dissertations and Theses (ETDs)

Cancer is a disease that has eluded medicinal approaches for many years and as a result new and improved therapeutic approaches are in constant demand. Although chemotherapy and radiation treatments have assisted in suppressing the growth of tumors, their poor selectivity and efficacy are major limitations for effective therapy en route towards the development of a cure for the cancer epidemic. With the mission of conquering cancer at heart, researchers have pursued a new form of cancer therapy, aptly named, a cancer targeting approach. This method revolves around the selection of a suitable biomarker, typically a cell surface receptor overexpressed ...


Detection Of Microbial Catecholamines In A Model Wound Environment, Ryan F. Wasurick 2014 California Polytechnic State University

Detection Of Microbial Catecholamines In A Model Wound Environment, Ryan F. Wasurick

STEM Teacher and Researcher (STAR) Program Posters

Chronic wounds are defined as an injury that does not heal in a predictable pattern or time frame. The healing process is composed of specific stages and chronic wounds have been interrupted in one or more stages of this process. This project focuses on the bacterial component of an infected wound, specifically on the catecholamines influencing the bacteria in the wound site. The assay developed in this experiment is designed to optimally grow bacteria in a simulated eukaryotic wound environment, and evaluate biofilm expression and catecholamine production of the bacteria. Bacteria used in this study are Staphylococcus aureus and Pseudomonas ...


Role Of Msaa Gene In Regulation Of The Msaabcr Operon And Biofilm Development In Staphylococcus Aureus, Ahmed Alzuway 2014 The University of Southern Mississippi

Role Of Msaa Gene In Regulation Of The Msaabcr Operon And Biofilm Development In Staphylococcus Aureus, Ahmed Alzuway

Master's Theses

Staphylococcus aureus is an important human pathogen that causes wide variety of diseases ranging from chronic biofilm associated infection to acute life threatening infection such as bacteremia, pneumonia, osteomyelitis, or endocarditis, despite the progress with antibiotics used in the treatment of bacterial infections. Furthermore, increased use of prosthetic and indwelling devices in modern medical practices has led to increased infections due to S. aureus. Treating S. aureus infections have become difficult owing to its ability to resist most of the antibiotics; this problem is further exacerbated by ability of MRSA strains to form biofilms. Emergence of community-acquired methicillin resistance staphylococcus ...


Identification And Characterization Of Msab Gene Involved In Biofilm Formation And Virulence In Staphylococcus Aureus, Amelsaad Elbarasi 2014 The University of Southern Mississippi

Identification And Characterization Of Msab Gene Involved In Biofilm Formation And Virulence In Staphylococcus Aureus, Amelsaad Elbarasi

Master's Theses

Staphylococcus aureus is an important human pathogen that causes a wide variety of life-threatening infections ranging from minor skin and oral infections to severe infections, such as bacteremia, pneumonia, osteomyelitis, or endocarditis due to the presence and secretion of a large number of virulence factors that are controlled by global virulence regulators in complex networks. Furthermore, S. aureus infections have become a threat to public health because of their high potential to form biofilm, and their ability to resist a wide range of antibiotics has exacerbated further. Therefore, understanding the regulatory networks and developing a drug targeting these networks has ...


Roles For B-Raf Kinase In The Specific Regulation Of Α4Β1 Integrin In T Cells, Wells S. Brown 2014 Texas Medical Center Library

Roles For B-Raf Kinase In The Specific Regulation Of Α4Β1 Integrin In T Cells, Wells S. Brown

UT GSBS Dissertations and Theses (Open Access)

The regulation of integrin-mediated adhesion is of vital importance to adaptive and innate immunity. Integrins are versatile proteins and mediate T cell migration and trafficking by binding to ECM or other cells, as well as initiating intracellular signaling cascades promoting survival or activation. The mitogen activated-protein kinase (MAPK) pathway is known to be downstream from integrins and regulate survival, differentiation, and motility. However, secondary roles for canonical MAPK pathway members are being discovered. We show chemical inhibition of RAF by Sorafenib or shRNA-mediated knockdown of B-Raf reduces T cell resistance to shear stress to α4β1 integrin ligands vascular cell adhesion ...


Egfr Modulates Microrna Maturation In Response To Hypoxia Through Phosphorylation Of Argonaute2, Jia Shen 2014 Texas Medical Center Library

Egfr Modulates Microrna Maturation In Response To Hypoxia Through Phosphorylation Of Argonaute2, Jia Shen

UT GSBS Dissertations and Theses (Open Access)

MicroRNAs (miRNAs) are generated by two-step processing to yield small RNAs that negatively regulate target gene expression at posttranscriptional level. Deregulation of miRNAs has been linked to diverse pathological processes, including cancer. Recent studies have also implicated miRNAs in regulatory roles to cope with a spectrum of stresses, such as hypoxia, which is frequently encountered in the poorly angiogenic core of a solid tumor. However, the upstream regulators of miRNA biogenesis machineries remain obscure, raising the question of how tumor cells efficiently coordinate and impose specificity on miRNA expression and function in response to stresses. Here, we show that EGFR ...


Cd56-Specific T Cells: Using Genetically Engineered T Cells To Redirect Specificity To A T Cell Expressed Antigen, Denise L. Crossland 2014 Texas Medical Center Library

Cd56-Specific T Cells: Using Genetically Engineered T Cells To Redirect Specificity To A T Cell Expressed Antigen, Denise L. Crossland

UT GSBS Dissertations and Theses (Open Access)

The CD56 antigen is expressed on several deadly malignancies currently lacking long-term efficacious therapies. Chimeric antigen receptor (CAR) based immunotherapies have shown both safety and efficacy and even a curative ability in clinical trials, laying the foundation for applying CARs to new targets. Using T cells to target a T cell expressed antigen, such as CD56, seems counterintuitive in that the T cells would be susceptible to self-targeting a.k.a. fratricide. However, we expand CD56-specific CAR+ T cells that co-express the CD56 antigen. Since other CARs targeting T cell expressed antigens are hypothesized to be undergoing fratricide, such as ...


Controlling Pseudomonas Aeruginosa Biofilms And Persister Cells By Manipulating Cell-Cell Signaling, Shuyuan Ma 2014 Syracuse University

Controlling Pseudomonas Aeruginosa Biofilms And Persister Cells By Manipulating Cell-Cell Signaling, Shuyuan Ma

Theses - ALL

Bacterial cells often form sessile biofilms that are up to 1,000 times more resistant to antimicrobial agents than free-living cells. Meanwhile, bacteria produce a small subpopulation of slow-growing or non-growing persister cells that exhibit high tolerance to antibiotics. Both biofilms and persister cells play important roles in the recalcitrance of chronic infections. Recent studies have shown that bacterial cell-to-cell communication, named quorum sensing (QS), is involved in the biofilm and persister formation.

In this study, we investigated the effects of quorum sensing signals N-(3-oxododecanoyl)-homoserine lactone (3-oxo-C12-HSL) and N-butyryl homoserine lactone (C4-HSL) on isolated Pseudomonas aeruginosa PAO1 and ...


Effects Of Leptin On Established Glioblastoma Cell Lines, Nicholas J. Cook 2014 Northern Michigan University

Effects Of Leptin On Established Glioblastoma Cell Lines, Nicholas J. Cook

NMU Master's Theses

Glioblastoma is one of the most difficult cancers to treat because it is aggressive and resistant to therapy. The discovery of new therapeutic targets is drastically needed as zero improved treatment options have been added to the standard of care over the past 15 years. New and promising therapeutic targets are arising from psychosocial and environmental enrichment studies examining the role of stress in cancer progression. In animal models, eustress appears to slow tumor growth and recurrence resulting in increased overall survival and progression free survival while distress is associated with decreased overall survival. The cellular pathways activated by eustress ...


Ngn3 Expression In Definitive Endoderm Progenitors Specifies Pancreatic Endocrine Precursors, Ahmed Shah 2014 Western University

Ngn3 Expression In Definitive Endoderm Progenitors Specifies Pancreatic Endocrine Precursors, Ahmed Shah

University of Western Ontario - Electronic Thesis and Dissertation Repository

Our lab previously demonstrated that expression of the lineage-determining transcription factor SOX17 in human embryonic stem cells was sufficient to specify stable definitive endoderm progenitors (DEPs). The current study was aimed at generating pancreatic precursors from SOX17-DEPs through controlled expression of the transcription factor NGN3. We generated hESC lines with inducible SOX17 and NGN3 and compared the effects of high and low levels of NGN3 expression. NGN3 expression in DEPs induced markers of pancreatic differentiation (PAX4, PAX6, NKX6.1, ISL1). While high NGN3 expression induced the β cell marker PDX1, low NGN3 expression induced the δ cell markers (CCKB ...


The Role Of C-Kit Receptor Tyrosine Kinase In Beta-Cell Proliferation, Function And Survival, Zhi Chao Feng 2014 Western University

The Role Of C-Kit Receptor Tyrosine Kinase In Beta-Cell Proliferation, Function And Survival, Zhi Chao Feng

University of Western Ontario - Electronic Thesis and Dissertation Repository

c-Kit, a receptor tyrosine kinase, interacts with Stem Cell Factor (SCF), mediating cell differentiation, function, and survival. c-Kit is critical for the development and maintenance of beta-cell function in both rodents and humans. The mutation of c-Kit at W locus (c-KitWv/+) in mice results in an early onset of diabetes. However, the underlying mechanisms by which c-Kit deficiency leads to beta-cell failure are unknown. Therefore, studying SCF/c-Kit downstream signaling pathways is essential to understanding the precise mechanism by which c-Kit regulates beta-cell survival and function in vivo.

We identified that dysregulated Akt/Glycogen synthase kinase 3β (Gsk3β)/cyclin ...


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