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Cellular Localization And Metabolomic Analysis Of The Arabidopsis Thaliana Major Intrinsic Protein Nip2;1: A Root-Specific Lactic Acid Transporter Induced In Response To Hypoxic Stress, Taylor K. Fuller 2015 University of Tennessee - Knoxville

Cellular Localization And Metabolomic Analysis Of The Arabidopsis Thaliana Major Intrinsic Protein Nip2;1: A Root-Specific Lactic Acid Transporter Induced In Response To Hypoxic Stress, Taylor K. Fuller

University of Tennessee Honors Thesis Projects

No abstract provided.


The Role Of The Pleckstrin Homology Domain-Containing Protein Ckip-1 In Activation Of P21-Activated Kinase 1 (Pak1), Yong-Bae Kim, Yong Jae Shin, Adhiraj Roy, Jeong-Ho Kim 2015 George Washington University

The Role Of The Pleckstrin Homology Domain-Containing Protein Ckip-1 In Activation Of P21-Activated Kinase 1 (Pak1), Yong-Bae Kim, Yong Jae Shin, Adhiraj Roy, Jeong-Ho Kim

Biochemistry and Molecular Medicine Faculty Publications

Upon growth factor stimulation, PAK1 is recruited to the plasma membrane and activated by a mechanism that requires its phosphorylation at S223 by the protein kinase CK2. However, the upstream signaling molecules that regulate this phosphorylation event are not clearly defined. Here, we demonstrate a major role of the CK2α-interacting protein CKIP-1 in activation of PAK1. CK2α, CKIP-1 and PAK1 are translocated to membrane ruffles in response to the epidermal growth factor (EGF), where CKIP-1 mediates the interaction between CK2α, and PAK1 in a PI3K-dependent manner. Consistently, we observe that PAK1 mediates phosphorylation and modulation of the activity of p41-Arc ...


Methods Of Detecting Target Nucleic Acids, amyi austin 2015 SelectedWorks

Methods Of Detecting Target Nucleic Acids, Amyi Austin

amyi austin

This paper talks about the methods of measuring target nucleic acids,which includes 5 sections:1.abstract, 2.what to claim,3.background, 4. summary, 5.note


Improving Alternate Lignin Catabolite Utilization Of Ligab From Sphingobium Sp. Strain Syk-6 Through Site Directed Mutagenesis, Kevin P. Barry, Erin F. Cohn, Abraham Ngu, Erika A. Taylor 2015 Wesleyan University

Improving Alternate Lignin Catabolite Utilization Of Ligab From Sphingobium Sp. Strain Syk-6 Through Site Directed Mutagenesis, Kevin P. Barry, Erin F. Cohn, Abraham Ngu, Erika A. Taylor

Erika A. Taylor, Ph.D.

Protocatechuate 4,5-dioxygenase (LigAB) catalyzes dioxygenation of multiple lignin derived aromatic compounds—such as protocatechuate (PCA), gallate (GA) and 3-O-methyl gallate (3OMG)—with decreasing proficiency as the molecule size increases. We predicted that phenylalanine-103 of the α subunit (Phe103α) controls substrate specificity through interaction with the C5-funtionality of bound substrates, and mutagenesis would enhance GA and 3OMG catalysis. LigAB with Phe103α mutations (F103 V, F103T and F103H) displayed enhanced catalytic efficiency for dioxygenation of 3OMG, with mutants displaying 12- to 31-fold increases in View the MathML source, making these mutant enzymes more active with 3OMG than its native dioxygenase (DesZ ...


Viral Non-Coding Rna Inhibits Hnf4Α Expression In Hcv Associated Hepatocellular Carcinoma, Zhao Wang, Kristin Ceniccola, Liliana Florea, Bi-Dar Wang, Norman H. Lee, Ajit Kumar 2015 George Washington University

Viral Non-Coding Rna Inhibits Hnf4Α Expression In Hcv Associated Hepatocellular Carcinoma, Zhao Wang, Kristin Ceniccola, Liliana Florea, Bi-Dar Wang, Norman H. Lee, Ajit Kumar

Biochemistry and Molecular Medicine Faculty Publications

Background

Hepatitis C virus (HCV) infection is an established cause of chronic hepatitis, cirrhosis and hepatocellular carcinoma (HCC); however, it is unclear if the virus plays a direct role in the development of HCC. Hepatocyte nuclear factor 4α (HNF4α) is critical determinant of epithelial architecture and hepatic development; depletion of HNF4α is correlated with oncogenic transformation. We explored the viral role in the inhibition of HNF4α expression, and consequent induction of tumor-promoting genes in HCV infection-associated HCC.

Methods

Western blot analysis was used to monitor the changes in expression levels of oncogenic proteins in liver tissues from HCV-infected humanized mice ...


World-Class Research: Biomedical Science Beyond Experimentation, 2015 Colby College

World-Class Research: Biomedical Science Beyond Experimentation

Colby Magazine

Faculty and relationships with Maine’s big-three research labs propel Colby’s opportunities in biomedical science

Could childhood nutrition set late-life brain function?

Hitting the lab running

Ahmad wins grant for study of neurodegenerative disease


Chemical Biology Of Protein Arginine Modifications In Epigenetic Regulation, Jakob Fuhrmann, Kathleen W. Clancy, Paul R. Thompson 2015 The Scripps Research Institute

Chemical Biology Of Protein Arginine Modifications In Epigenetic Regulation, Jakob Fuhrmann, Kathleen W. Clancy, Paul R. Thompson

Thompson Lab Publications

Review article on histone citrullination (arginine deimination), histone arginine methylation, and noncanonical histone arginine modifications.


The Effect Of Osm On Mc3t3-E1 Osteoblastic Cells In Simulated Microgravity With Radiation, Jake Goyden, Ken Tawara, Danielle Hedeen, Jeffrey S. Willey, Julia Thom Oxford, Cheryl L. Jorcyk 2015 Boise State University

The Effect Of Osm On Mc3t3-E1 Osteoblastic Cells In Simulated Microgravity With Radiation, Jake Goyden, Ken Tawara, Danielle Hedeen, Jeffrey S. Willey, Julia Thom Oxford, Cheryl L. Jorcyk

Biomolecular Research Center Publications and Presentations

Bone deterioration is a challenge in long-term spaceflight with significant connections to patients experiencing disuse bone loss. Prolonged unloading and radiation exposure, defining characteristics of space travel, have both been associated with changes in inflammatory signaling via IL-6 class cytokines in bone. While there is also evidence for perturbed IL-6 class signaling in spaceflight, there has been scant examination of the connections between microgravity, radiation, and inflammatory stimuli in bone. Our lab and others have shown that the IL-6 class cytokine oncostatin M (OSM) is an important regulator of bone remodeling. We hypothesize that simulated microgravity alters osteoblast OSM signaling ...


Chronic Inflammation As A Result Of Hepatitis C Virus Infection: A Review Of The Literature, Samantha L. Lane 2015 DePaul University

Chronic Inflammation As A Result Of Hepatitis C Virus Infection: A Review Of The Literature, Samantha L. Lane

DePaul Discoveries

Approximately 170 million people are infected with Hepatitis C virus (HCV) worldwide5,6. It is estimated that roughly 80% of those infected suffer from persistent infection with the virus; this persistence of infection is progressive, and over time can lead to fibrosis, cirrhosis, and hepatocellular carcinoma7. Chronic inflammation and apoptotic deregulation are both hallmarks of chronic HCV infection, and many molecular pathways are initiated in both the innate and adaptive immune responses during infection with this viral pathogen. The aim of this review was to survey some of the major molecular mechanisms responsible for the induction of chronic ...


Pharmacology Of Organic Cation Transporters: Focus On Structure-Function Relationships In Oct3 (Slc22a3), Dan C. Li 2015 Washington University in St Louis

Pharmacology Of Organic Cation Transporters: Focus On Structure-Function Relationships In Oct3 (Slc22a3), Dan C. Li

Undergraduate Theses—Unrestricted

Organic Cation Transporters (OCTs) are polyspecific, facilitative transporters that play major roles in metabolite and drug clearance. OCTs are promising drug targets and elucidating their mechanisms of substrate recognition is crucial for rational drug design. OCT-mediated transport of polyvalent cations remains unexplored. OCT-expressing Xenopus laevis oocytes were used to assess transport of polyamines, ubiquitous polyvalent cations of broad physiological import, but for which transport mechanisms are unknown. Dose-response analysis of radiolabelled substrate uptake revealed that polyamines are relatively low affinity, but high turnover substrates for OCTs compared to model substrate methyl-4-phenylpyridinium (MPP+). Polyamine analogs of varying hydrophobic character were screened ...


Three-Dimensional Ideal Gas Reference State Based Energy Function, Avdesh Mishra 2015 University of New Orleans

Three-Dimensional Ideal Gas Reference State Based Energy Function, Avdesh Mishra

University of New Orleans Theses and Dissertations

Energy functions are found to be a key of protein structure prediction. In this work, we propose a novel 3-dimensional energy function based on hydrophobic-hydrophilic properties of amino acid where we consider at least three different possible interaction of amino acid in a 3-dimensional sphere categorized as hydrophilic versus hydrophilic, hydrophobic versus hydrophobic and hydrophobic versus hydrophilic. Each of these interactions are governed by a 3-dimensional parameter alpha used to model the interaction and 3-dimensional parameter beta used to model weight of contribution. We use Genetic Algorithm (GA) to optimize the value of alpha, beta and Z-score. We obtain three ...


Reverse Gyrase Is Not Necessary For Survival Of Hyperthermophilic Archaeon Pyrococcus Furiosus, Farshid Taghizadeh, Michael S. Bartlett 2015 Portland State University

Reverse Gyrase Is Not Necessary For Survival Of Hyperthermophilic Archaeon Pyrococcus Furiosus, Farshid Taghizadeh, Michael S. Bartlett

Student Research Symposium

Reverse gyrase is the only known topoisomerase enzyme with positive supercoiling activity on covalently-closed DNA. This positive supercoiling is required to prevent DNA from denaturation at high temperatures. The gene that codes for this protein is present in all hyperthermophiles and absent from all mesophilic and thermophilic genomes, suggesting that this enzyme is the only hyperthermophile-specific protein. To investigate if this protein is vital for the cells, we knocked out its gene from the genome of living organism Pyrococcus furiosus. Pyrococcus furiosus is a hyperthermophilic archaeon that grows between 70°C to 103°C with an optimum growth temperature of ...


Fishing For A Sustainable Future: Aquaponics As A Method Of Food Production, Richard Ramsundar 2015 Fordham University

Fishing For A Sustainable Future: Aquaponics As A Method Of Food Production, Richard Ramsundar

Student Theses 2015-Present

This thesis compares and explains the advantages aquaponics farming has over modern industrial intensive farming. Through a comparison natural capital usage, conservation, recycling and cost, the thesis advocates for the expansion of aquaponics usage in urban settings. The thesis also explains the history of intensive farming and aquaponics in America, the science of how aquaponics operates, the economic and environmental costs of modern intensive farming versus aquaponics farming, and the social implications of aquaponics. Lastly, I propose a policy that reallocates farm subsidies by modifying the Farm Bill. Then I propose policies that support creating a new standard of farm ...


Cell And Molecular Biology: What We Know & How We Found Out (Sample Chapter), Gerald Bergtrom 2015 University of Wisconsin - Milwaukee

Cell And Molecular Biology: What We Know & How We Found Out (Sample Chapter), Gerald Bergtrom

Cell and Molecular Biology i-Text

No abstract provided.


Cell And Molecular Biology: What We Know & How We Found Out (Basic Itext), Gerald Bergtrom 2015 University of Wisconsin - Milwaukee

Cell And Molecular Biology: What We Know & How We Found Out (Basic Itext), Gerald Bergtrom

Cell and Molecular Biology i-Text

No abstract provided.


Cell And Molecular Biology: What We Know & How We Found Out (Annotated Itext), Gerald Bergtrom 2015 University of Wisconsin - Milwaukee

Cell And Molecular Biology: What We Know & How We Found Out (Annotated Itext), Gerald Bergtrom

Cell and Molecular Biology i-Text

No abstract provided.


Characterization Of A Novel Clade Of Transporters In Phytophthora, Stephanie Padula, Paul F. Morris Dr, Howard Casey Cromwell Dr., Menaka Ariyaratne, Andrew Wagner 2015 Bowling Green State University - Main Campus

Characterization Of A Novel Clade Of Transporters In Phytophthora, Stephanie Padula, Paul F. Morris Dr, Howard Casey Cromwell Dr., Menaka Ariyaratne, Andrew Wagner

Honors Projects

The oomycete Phytophthora parasitica has a worldwide distribution and is an economically important pathogen of more than 100 species4. RNA-seq analysis showed that one gene, PPTG_16698 has the 5th highest level of expression of all transport proteins in the zoospore stage, and is highly conserved throughout Phytophthora species. This project attempts to characterize the important biological role that PPTG_16698 plays in P. parasitica and other oomycetes. Three strategies have been implemented to accomplish this goal: growth analysis by heterologous expression in yeast, metabolite analysis in yeast, and construction of a GFP fusion protein to enable localization of the ...


Efficient In Vitro Development Of Photoreceptors From Human Pluripotent Stem Cells, Joseph C. Reynolds 2015 Dominican University of California

Efficient In Vitro Development Of Photoreceptors From Human Pluripotent Stem Cells, Joseph C. Reynolds

Master's Theses and Capstone Projects

Degeneration of the rod and cone photoreceptors in the human retina is among the most common causes of blindness. Replacing these damaged photoreceptors may help to restore vision. Repairing the damaged retina relies on the insertion of new, healthy cells. Embryonic stem (ES) cells and induced pluripotent stem (iPS) cells are two possible sources of photoreceptors to restore vision. Previous data shows that human ES cells and iPS cells can be differentiated into photoreceptors and transplanted into the eye to restore some vision. However, this process is inefficient, and costly. Here, we show a new method for inducing photoreceptor production ...


Investigations Into The Molecular Mechanisms Of Bacterial Pathogen-Host Interactions: Construction Of A Dual Plasmid System For Incorporation Of Unnatural Amino Acids Into Pseudomonas Syringae Pv. Tomato Dc3000, Scotty D. Raber 2015 University of Nebraska-Lincoln

Investigations Into The Molecular Mechanisms Of Bacterial Pathogen-Host Interactions: Construction Of A Dual Plasmid System For Incorporation Of Unnatural Amino Acids Into Pseudomonas Syringae Pv. Tomato Dc3000, Scotty D. Raber

Student Research Projects, Dissertations, and Theses - Chemistry Department

A dual plasmid system for the incorporation of unnatural amino acids into plant pathogen, Pseudomonas syringae pv. tomato DC3000, has been designed. This invention is expected to allow (a) mutations of proteins synthesized by the bacterium, P. syringae pv. tomato DC3000, that can capture molecular targets, especially for such modified proteins secreted by the phytopathogen into the host plant cells of A. thaliana and S. lycopersicum, (b) expression of biological probes in the bacterial species to monitor changes in redox, nutritional, and other small molecule states over pre-, post- and in situ disease stages, and (c) secretion of such biological ...


The Characterization Of Amyloid Fibrils And Novel Synthetic Heparin-Binding Peptides Binding To Cell Surfaces, Nicole Marie Hackenbrack 2015 University of Tennessee, Knoxville

The Characterization Of Amyloid Fibrils And Novel Synthetic Heparin-Binding Peptides Binding To Cell Surfaces, Nicole Marie Hackenbrack

University of Tennessee Honors Thesis Projects

No abstract provided.


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