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Tea Induction Of Cyp6a8 Promoter In Drosophila Melanogaster, Fiona Retzer 2017 University of Tennessee, Knoxville

Tea Induction Of Cyp6a8 Promoter In Drosophila Melanogaster, Fiona Retzer

University of Tennessee Honors Thesis Projects

No abstract provided.


Session C-1: Modeling Stem Activities Into Classroom Practice, Sowmya Anjur 2017 Illinois Mathematics and Science Academy

Session C-1: Modeling Stem Activities Into Classroom Practice, Sowmya Anjur

Professional Learning Day

Students understand concepts better when they have had a chance to work hands on with relevant material. Examples will be presented from my classroom where difficult concepts have been modeled into simple experiments with considerable success in enhancing student understanding. Special focus will be given to selected topics that students seem to have the most difficulty grasping. The objective is to enable students to transfer their understanding to solve complex problems with considerable ease and apply their understanding to real world scenarios on assessments. Suggestions will also be provided for implementation of various concepts into the high school classroom.


Co2-Fixing One-Carbon Metabolism In A Cellulose-Degrading Bacterium Clostridium Thermocellum, Wei Xiong, Paul P. Lin, Lisa Warner, James C. Liao, Pin-Ching Maness, Katherine J. Chou 2017 National Renewable Energy Labratory

Co2-Fixing One-Carbon Metabolism In A Cellulose-Degrading Bacterium Clostridium Thermocellum, Wei Xiong, Paul P. Lin, Lisa Warner, James C. Liao, Pin-Ching Maness, Katherine J. Chou

Lisa R. Warner

Clostridium thermocellum can ferment cellulosic biomass to formate and other end products, including CO2. This organism lacks formate dehydrogenase (Fdh), which catalyzes the reduction of CO2 to formate. However, feeding the bacterium 13C-bicarbonate and cellobiose followed by NMR analysis showed the production of 13C-formate in C. thermocellum culture, indicating the presence of an uncharacterized pathway capable of converting CO2 to formate. Combining genomic and experimental data, we demonstrated that the conversion of CO2 to formate serves as a CO2 entry point into the reductive one-carbon (C1) metabolism, and internalizes CO2 via two ...


The Effects Of High Concentrations Of Ionic Liquid On Gb1 Protein Structure And Dynamics Probed By High-Resolution Magic-Angle-Spinning Nmr Spectroscopy, Lisa Warner, Erica Gjersing, Shelby E. Follett, K. Wade Elliott, Sergei V. Dzyuba, Krisztina Varga 2017 Boise State University

The Effects Of High Concentrations Of Ionic Liquid On Gb1 Protein Structure And Dynamics Probed By High-Resolution Magic-Angle-Spinning Nmr Spectroscopy, Lisa Warner, Erica Gjersing, Shelby E. Follett, K. Wade Elliott, Sergei V. Dzyuba, Krisztina Varga

Lisa R. Warner

Ionic liquids have great potential in biological applications and biocatalysis, as some ionic liquids can stabilize proteins and enhance enzyme activity, while others have the opposite effect. However, on the molecular level, probing ionic liquid interactions with proteins, especially in solutions containing high concentrations of ionic liquids, has been challenging. In the present work the 13C, 15N-enriched GB1 model protein was used to demonstrate applicability of high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy to investigate ionic liquid–protein interactions. Effect of an ionic liquid (1-butyl-3- methylimidazolium bromide, [C4-mim]Br) on GB1was studied over a wide range of the ...


Structural Basis For The Recognition Of Spliceosomal Smn/B/B' Proteins By The Rbm5 Ocre Domain In Splicing Regulation, André Mourão, Sophie Bonnal, Komal Soni, Lisa Warner, Rémy Bordonné, Juan Valcárcel, Michael Sattler 2017 Helmholtz Zentrum München

Structural Basis For The Recognition Of Spliceosomal Smn/B/B' Proteins By The Rbm5 Ocre Domain In Splicing Regulation, André Mourão, Sophie Bonnal, Komal Soni, Lisa Warner, Rémy Bordonné, Juan Valcárcel, Michael Sattler

Lisa R. Warner

The multi-domain splicing factor RBM5 regulates the balance between antagonistic isoforms of the apoptosis-control genes FAS/CD95, Caspase-2 and AID. An OCRE (OCtamer REpeat of aromatic residues) domain found in RBM5 is important for alternative splicing regulation and mediates interactions with components of the U4/U6.U5 tri-snRNP. We show that the RBM5 OCRE domain adopts a unique β–sheet fold. NMR and biochemical experiments demonstrate that the OCRE domain directly binds to the proline-rich C-terminal tail of the essential snRNP core proteins SmN/B/B’. The NMR structure of an OCRE-SmN peptide complex reveals a specific recognition of poly-proline ...


Bioaccumulation Of Metallic Trace Elements And Antioxidant Enzyme Activities In Apfelbeckia Insculpta (L. Koch, 1867) (Diplopoda: Callipodida) From The Cave Hadži-Prodanova Pećina (Serbia), Jelena Vranković, Slavica Borković-Mitić, Bojan Ilić, Milanka Radulović, Slaviša Milošević, Slobodan Makarov, Bojan Mitić 2017 University of Belgrade

Bioaccumulation Of Metallic Trace Elements And Antioxidant Enzyme Activities In Apfelbeckia Insculpta (L. Koch, 1867) (Diplopoda: Callipodida) From The Cave Hadži-Prodanova Pećina (Serbia), Jelena Vranković, Slavica Borković-Mitić, Bojan Ilić, Milanka Radulović, Slaviša Milošević, Slobodan Makarov, Bojan Mitić

International Journal of Speleology

The concentration of 10 metallic trace elements or MTE (Cu, Fe, Zn, Mn, As, Hg, Pb, Cd, Ni, and Cr) was measured in specimens of the troglophilic millipede Apfelbeckia insculpta (L. Koch, 1867) and sediment of the cave Hadži-Prodanova Pećina in western Serbia. Some MTE, like Fe and Mn, displayed much higher concentrations compared to other elements, both in the sediment and in the body of A. insculpta. On the other hand, estimation of the bioaccumulation factor (BAF) in both males and females of A. insculpta showed values greater than 1 for xenobiotic elements compared to those that are essential ...


Quantitative Analysis Of Endocytosis Of Macrophage Growth Factor Receptor By Molecular Imaging, Victor Georgiev, Emily Toering, Christopher Callahan 2017 South Dakota State University

Quantitative Analysis Of Endocytosis Of Macrophage Growth Factor Receptor By Molecular Imaging, Victor Georgiev, Emily Toering, Christopher Callahan

The Journal of Undergraduate Research

Endocytosis is a critical cellular process responsible for nutrient uptake, sampling the environment, and cellular signaling. Endocytosis of growth factor receptors is an important step in the regulation of receptor signaling functions. The macrophage growth factor receptor controls growth and differentiation of macrophages, however, the pathway by which the receptor is internalized is not well understood. The analysis showed that for the first time, knockout of endophilin A2 decreases the internalization of macrophage growth factor receptor in primary bone marrow macrophages. This shows that fast endophilin-mediated endocytosis plays a role in the control of the amount of growth factor receptors ...


Diversity And Biocide Susceptibility Of Fungal Assemblages Dwelling In The Art Gallery Of Magura Cave, Bulgaria, Milena M. Mitova, Mihail Iliev, Alena Novakova, Anna A. Gorbushina, Veneta I. Groudeva, Pedro M. Martin-Sanchez 2017 Sofia University

Diversity And Biocide Susceptibility Of Fungal Assemblages Dwelling In The Art Gallery Of Magura Cave, Bulgaria, Milena M. Mitova, Mihail Iliev, Alena Novakova, Anna A. Gorbushina, Veneta I. Groudeva, Pedro M. Martin-Sanchez

International Journal of Speleology

Magura Cave, north-western Bulgaria, possesses valuable rock-art paintings made with bat guano and dated from the period between the Eneolithic and Bronze Ages. Since 2008, the Art Gallery is closed to the general public in order to protect the paintings from vandalism, microclimatic changes caused by visitors and artificial illumination, and the consequent growth of fungi and phototrophs. Nevertheless, some tourist visits are allowed under the supervision of cave managers. This study provides the first scientific report on cultivable fungal assemblages dwelling different substrata in the Art Gallery. A total of 78 strains, belonging to 37 OTUs (Ascomycota 81%, Zygomycota ...


Utilizing Microfluidics Devices To Monitor Endothelial Cell Migration., Tsz Ming Jeremy Tsang, Jacob Lefler, Charles Voyton, Kenneth Christensen 2017 Brigham Young University - Provo

Utilizing Microfluidics Devices To Monitor Endothelial Cell Migration., Tsz Ming Jeremy Tsang, Jacob Lefler, Charles Voyton, Kenneth Christensen

Biomedical Engineering Western Regional Conference

Utilizing microfluidics devices to monitor endothelial cell migration.


Engineering Fret Biosensors For Microrna Presence/Absence Analysis, Nicholas E. Larkey, Sean M. Burrows 2017 Oregon State University

Engineering Fret Biosensors For Microrna Presence/Absence Analysis, Nicholas E. Larkey, Sean M. Burrows

Biomedical Engineering Western Regional Conference

No abstract provided.


Chloride And Proton Binding In The E. Coli 2cl¯:1h+ Clc Exchanger, Catherine Chenal 2017 The Graduate Center, City University of New York

Chloride And Proton Binding In The E. Coli 2cl¯:1h+ Clc Exchanger, Catherine Chenal

All Graduate Works by Year: Dissertations, Theses, and Capstone Projects

The CLC family of membrane proteins is a ubiquitously expressed class of proton and usually voltage-activated chloride transporters involved in a myriad of physiological functions. Crystallographic structures identify up to three chloride binding sites: external, central and intracellular located in the inner half of the trans-membrane domain. The CLC proteins, except for the kidney isoforms, are gated by the extracellular side-facing gating Glutamate (Ex, E148 in CLC-ec1, the E. coli exchanger), which is thought to undergo a conformational change upon protonation.

To sort out how the thermodynamic paths to H+ coupled Cl¯ binding and conformational change in CLC-ec1 at the ...


Transcriptional And Post-Transcriptional Regulation Of Histone Variant H2a.Z During Sea Urchin Development, Mihai Hajdu 2017 The Graduate Center, City University of New York

Transcriptional And Post-Transcriptional Regulation Of Histone Variant H2a.Z During Sea Urchin Development, Mihai Hajdu

All Graduate Works by Year: Dissertations, Theses, and Capstone Projects

Histone variant H2A.Z promotes chromatin accessibility at transcriptional regulatory elements and is developmentally regulated in metazoans. We characterize the transcriptional and post-transcriptional regulation of H2A.Z in the purple sea urchin Strongylocentrotus purpuratus. H2A.Z depletion by antisense translation-blocking morpholino oligonucleotides during early development causes developmental collapse, in agreement with its previously demonstrated general role in transcriptional multipotency. During H2A.Z peak expression in 24-h embryos, endogenous H2A.Z 3’ UTR sequences stabilize GFP mRNAs relative to those with SV40 3’ UTR sequences, although the 3’UTR of H2A.Z does not determine the spatial distribution of H2A.Z ...


Extracting Biochemical Parameters From Protein Distributions Of Vascular Cells, Partha Srinivasan 2017 Cleveland State University

Extracting Biochemical Parameters From Protein Distributions Of Vascular Cells, Partha Srinivasan

Partha Srinivasan

No abstract provided.


Maysin And Its Flavonoid Derivative From Centipedegrass Attenuates Amyloid Plaques By Inducting Humoral Immune Response With Th2 Skewed Cytokine Response In The Tg (Appswe, Ps1de9) Alzheimer's Mouse Model, Yuno Song, Hong-Duck Kim, Min-Kwon Lee, Il-Hwa Hong, Chung-Kil Won, Seung Sik Lee, Jae-Hyeon Cho 2017 New York Medical College

Maysin And Its Flavonoid Derivative From Centipedegrass Attenuates Amyloid Plaques By Inducting Humoral Immune Response With Th2 Skewed Cytokine Response In The Tg (Appswe, Ps1de9) Alzheimer's Mouse Model, Yuno Song, Hong-Duck Kim, Min-Kwon Lee, Il-Hwa Hong, Chung-Kil Won, Seung Sik Lee, Jae-Hyeon Cho

NYMC Faculty Publications

Alzheimer's disease (AD) is a slow, progressive neurodegenerative disease and the most common type of dementia in the elderly. The etiology of AD and its underlying mechanism are still not clear. In a previous study, we found that an ethyl acetate extract of Centipedegrass (CG) (i.e., EA-CG) contained 4 types of Maysin derivatives, including Luteolin, Isoorientin, Rhamnosylisoorientin, and Derhamnosylmaysin, and showed protective effects against Amyloid beta (Aβ) by inhibiting oligomeric Aβ in cellular and in vitro models. Here, we examined the preventative effects of EA-CG treatment on the Aβ burden in the Tg (Mo/Hu APPswe PS1dE9) AD ...


Telomere Estimates By Qpcr In American Kestrel Birds, Esteban Palencia, Julie A. Heath (Mentor), Eric J. Hayden (Mentor) 2017 Boise State University

Telomere Estimates By Qpcr In American Kestrel Birds, Esteban Palencia, Julie A. Heath (Mentor), Eric J. Hayden (Mentor)

Idaho Conference on Undergraduate Research

Telomeres are highly conserved repeated sequences found at the ends of linear chromosomes. Measuring telomere length has been used to estimate the ages of individuals in several species. The research presented here focuses on developing telomere length estimates in the American Kestrel (Falco sparverius), using Quantitative Polymerase Chain Reaction (qPCR). The verification of a primer pair that can amplify a reference gene and serve to normalize the telomere qPCR data is vitally important. The gene for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was preferably chosen because it occurs in a single copy in most birds. However, the Kestrel genome has not been sequenced ...


Arsenite Resistance Of Euglena Mutabilis, Nicole Loumarinett Rosendo Mercado, Doug G. Cole (Mentor) 2017 University of Idaho

Arsenite Resistance Of Euglena Mutabilis, Nicole Loumarinett Rosendo Mercado, Doug G. Cole (Mentor)

Idaho Conference on Undergraduate Research

Euglena are photosynthetic unicellular protozoa. Commonly found in rivers, lakes and ponds, some Euglena are found in toxic environments, like areas of acid mine drainage where they can grow at low pH and in the presence of heavy metals and metalloids such as arsenite (e.g. Euglena mutabilis). The goal of this research is to determine if an Idaho Euglena isolate (SG6) belongs to the E. mutabilis species and to compare it’s level of arsenite resistance with other E. mutabilis strains. As part of the methodology, we have developed microtiter plate assays in which the cells are grown in ...


The Role Of Inflammasomes In Asbestos-Induced Mesothelial To Fibroblastic Transition, Joyce K. Thompson 2017 University of Vermont

The Role Of Inflammasomes In Asbestos-Induced Mesothelial To Fibroblastic Transition, Joyce K. Thompson

Graduate College Dissertations and Theses

Malignant Mesothelioma (MM) is a fatal disease with a low median survival between 8 to 12 months after diagnosis. MM has a long latency period (10-60 years), is causally related to asbestos exposure, and is refractory to all available modes of therapy. Despite the causal association between asbestos exposure and MM however, the mechanisms by which asbestos induces this deadly disease remain unclear. Chronic inflammation due to the presence of asbestos fibers is believed to play an important role in all aspects of MM pathogenesis, from development to progression and resistance. Chronic inflammation has been shown to promote dysregulated wound ...


Effects Of Novel Fragment-Warhead Adducts In Situ And In Vitro With Glutaredoxin Orthologs., Andrew J. Caras 2017 The University of Akron

Effects Of Novel Fragment-Warhead Adducts In Situ And In Vitro With Glutaredoxin Orthologs., Andrew J. Caras

Honors Research Projects

Fragment-based drug design (FBDD) is a method of pharmaceutical research involving characterization of small “fragment” molecule interactions with a target protein. These fragments are optimized with the addition of a nucleophilic “warhead” moiety. Several novel fragment-warhead adducts have been synthesized in house by group member Ram Khattri Ph.D. The experiment investigates reactivity of these adducts with glutaredoxin protein (Grx) orthologs from Homo sapiens, Pseudomonas aeruginosa and Brucella melitensis. Three novel fragment-warhead adducts have been identified to cause weakly bacteriostatic and bacteriocidal effects, most with minimum inhibitory concentration values near 1.28 mg/mL. Discussed herein are molecular effects of ...


Metabolic Reprogramming Of Human Lung Cancer Cells And Ex Vivo Tissues Revealed By Uhr-Ftms Analysis Of Small Amino And Carboxyl Metabolites, Ye Yang 2017 University of Kentucky

Metabolic Reprogramming Of Human Lung Cancer Cells And Ex Vivo Tissues Revealed By Uhr-Ftms Analysis Of Small Amino And Carboxyl Metabolites, Ye Yang

Theses and Dissertations--Toxicology and Cancer Biology

Studies were carried out to understand how human lung cancer cells and human ex vivo lung cancer tissues that are metabolically reprogramed compared with analogous non-cancer cells or non-cancer tissues. A Stable isotope resolved metabolomics (SIRM) approach was used to fulfill this aim by employing 13C, 2H or 15N labeled metabolic precursors like 13C6-Glc, 13C2-Gly, 2H2-Gly, 2H3-Ser, 13C5, 15N2-Gln to trace the flow of the labeled atoms into the down stream metabolic network. NMR and mass spectrometry are two major ...


Mrub_2642, Mrub_1054, And Mrub_1059 Genes Are Orthologs Of The Escherichia Coli Genes B2942, B0159, And B2687 Genes, Respectively, Which Code For Methionine Adenosyltransferase, Adenosylhomocysteine Nucleosidase, And S-Ribosylhomocysteine Lyase, Nicholas M. Orslini, Dr. Lori R. Scott 2017 Augustana College, Rock Island Illinois

Mrub_2642, Mrub_1054, And Mrub_1059 Genes Are Orthologs Of The Escherichia Coli Genes B2942, B0159, And B2687 Genes, Respectively, Which Code For Methionine Adenosyltransferase, Adenosylhomocysteine Nucleosidase, And S-Ribosylhomocysteine Lyase, Nicholas M. Orslini, Dr. Lori R. Scott

Meiothermus ruber Genome Analysis Project

This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation –Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of the genes Mrub_2642, Mrub_1054, and Mrub_1059.

We predict that Mrub_2642 encodes the enzyme methionine adenosyltransferase (DNA coordinates [2677251…2678426] on the reverse strand), the first step of the methionine degradation pathway (KEGG map number 00270). Methionine adenosyltransferase catalyzes the conversion of the substrates, ATP, L-methionine, and water, to yield the products S-adenosyl-L-methionine (SAM), inorganic phosphate, and diphosphate. Mrub_1054 encodes adenosylhomocysteine nucleosidase (DNA ...


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