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Cell And Molecular Biology : What We Know & How We Found Out (Second Edition, Sample Chapter), Gerald Bergtrom 2016 University of Wisconsin - Milwaukee

Cell And Molecular Biology : What We Know & How We Found Out (Second Edition, Sample Chapter), Gerald Bergtrom

Cell and Molecular Biology 2e i-Text

No abstract provided.


Cell And Molecular Biology : What We Know & How We Found It (Second Edition, An Annotated Itext), Gerald Bergtrom 2016 University of Wisconsin - Milwaukee

Cell And Molecular Biology : What We Know & How We Found It (Second Edition, An Annotated Itext), Gerald Bergtrom

Cell and Molecular Biology 2e i-Text

No abstract provided.


Dietary Weight Loss Advice In Us Health Magazines And Its Relation To Ancestral Diet, Risa J. Stein, Marium A. Choudhry, Thomas J. Murray Jr. 2016 Rockhurst University

Dietary Weight Loss Advice In Us Health Magazines And Its Relation To Ancestral Diet, Risa J. Stein, Marium A. Choudhry, Thomas J. Murray Jr.

Journal of Evolution and Health

As rates of overweight and obesity have risen in the US, the public has sought effective strategies for weight loss through dietary modification. A proliferation of processed foods and changing governmental nutrition guidelines have both impacted dietary intake patterns. While physicians are considered respectable sources of weight-loss information, increasingly the public has turned to the media, particularly magazines, for weight loss advice. This study investigated the dietary recommendations found in the five leading US health-related magazines and compared those recommendations to ancestral diets. With a couple notable exceptions, leading health magazines present consistent recommendations for dietary modifications to promote weight ...


Quantifying Greenhouse Gas Flux Of Restored Vs. Unrestored Wetlands: A Case Study At Prairie Wolf Slough, Matthew Connors, Beth Lawrence 2016 DePaul University

Quantifying Greenhouse Gas Flux Of Restored Vs. Unrestored Wetlands: A Case Study At Prairie Wolf Slough, Matthew Connors, Beth Lawrence

DePaul Discoveries

Wetlands provide ecological services such as cleansing the water supply, sequestering carbon, and providing habitat for wildlife, however wetland restoration often alters the greenhouse gas flux of the site. Our study aims to investigate the effects of wetland restoration on greenhouse gas flux at Prairie Wolf Slough. We did this by comparing greenhouse gas flux on matching hydric soil series from the restored wetland with an adjacent abandoned agricultural field. We measured known controls of greenhouse gas flux such as soil moisture and soil temperature. We found that there was no detectable methane and nitrous oxide flux at either site ...


Multiplexed Detection Of Double-Stranded Pathogenic Dna With Engineered Zinc Finger Proteins, Juhwa Kim 2016 Western Kentucky University

Multiplexed Detection Of Double-Stranded Pathogenic Dna With Engineered Zinc Finger Proteins, Juhwa Kim

Masters Theses & Specialist Projects

The development of a new technology for the detection of doublestranded (ds) DNA enables multiple biomedical applications including identifying multiple pathogens simultaneously. We previously employed colorimetric SEquence-Enabled Reassembly with TEM-1 β-lacatamase (SEER-LAC) to detect specific bacterial DNA sequence. SEER-Lac consists of the two inactive β-lactamase fragments which of each attached to a zinc finger protein (ZFP) would reassemble into an active full-length enzyme upon ZFPs binding to its target DNA. Here, we engineered two pairs of ZFPs which of each recognizes shiga toxin in E. coli O157 and staphylococcal enterotoxin B in Staphylococus Aureus, respectively. Biotin was simply conjugated to ...


Trimethylated Lysine 4 At Histone 3 Shows The Same Circadian Rhythm At Promoters Of Diversely-Expressed Genes In Chlamydomonas Reinhardtii, Robyn M. Wilson 2016 Western Kentucky University

Trimethylated Lysine 4 At Histone 3 Shows The Same Circadian Rhythm At Promoters Of Diversely-Expressed Genes In Chlamydomonas Reinhardtii, Robyn M. Wilson

Masters Theses & Specialist Projects

Circadian clocks are biochemical mechanisms that allow eukaryotic and some prokaryotic organisms to coordinate their physiology with daily environmental changes. It enables organisms to increase their fitness by taking advantage of beneficial environmental conditions while also avoiding or restricting certain sensitive processes during harsh conditions. Similarly, post-translational histone modifications allow eukaryotic organisms to regulate gene expression in response to environmental or developmental factors. Some post-translational modifications of histones are associated with active transcription while others are associated with repressed transcription depending upon the location, type and degree of modification. Trimethylation of lysine 4 on the N-terminal tail of histone H3 ...


Bauer Ketones 23 And 24 From Echinacea Paradoxa Var. Paradoxa Inhibit Lipopolysaccharide-Induced Nitric Oxide, Prostaglandin E2 And Cytokines In Raw264.7 Mouse Macrophages, Xiaozhu Zhang, Ludmila Rizshsky, Catherine C. Hauck, Luping Qu, Mark P. Widrlechner, Basil J. Nikolau, Patricia A. Murphy, Diane F. Birt 2016 Iowa State University

Bauer Ketones 23 And 24 From Echinacea Paradoxa Var. Paradoxa Inhibit Lipopolysaccharide-Induced Nitric Oxide, Prostaglandin E2 And Cytokines In Raw264.7 Mouse Macrophages, Xiaozhu Zhang, Ludmila Rizshsky, Catherine C. Hauck, Luping Qu, Mark P. Widrlechner, Basil J. Nikolau, Patricia A. Murphy, Diane F. Birt

Basil Nikolau

Among the nine Echinacea species, E. purpurea, E. angustifolia and E. pallida, have been widely used to treat the common cold, flu and other infections. In this study, ethanol extracts of these three Echinaceaspecies and E. paradoxa, including its typical variety, E. paradoxa var. paradoxa, were screened in lipopolysaccharide (LPS)-stimulated macrophage cells to assess potential anti-inflammatory activity. E. paradoxa var. paradoxa, rich in polyenes/polyacetylenes, was an especially efficient inhibitor of LPS-induced production of nitric oxide (NO), prostaglandin E2 (PGE2), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) by 46%, 32%, 53% and 26%, respectively, when tested at 20 μg ...


Enrichment Of Echinacea Angustifolia With Bauer Alkylamide 11 And Bauer Ketone 23 Increased Anti-Inflammatory Potential Through Interference With Cox-2 Enzyme Activity, Carlie A. LaLone, Nan Huang, Ludmila Rizshsky, Man-Yu Yum, Navrozedeep Singh, Cathy Hauck, Basil J. Nikolau, Eve S. Wurtele, Marian L. Kohut, Patricia A. Murphy, Diane F. Birt 2016 Iowa State University

Enrichment Of Echinacea Angustifolia With Bauer Alkylamide 11 And Bauer Ketone 23 Increased Anti-Inflammatory Potential Through Interference With Cox-2 Enzyme Activity, Carlie A. Lalone, Nan Huang, Ludmila Rizshsky, Man-Yu Yum, Navrozedeep Singh, Cathy Hauck, Basil J. Nikolau, Eve S. Wurtele, Marian L. Kohut, Patricia A. Murphy, Diane F. Birt

Basil Nikolau

Bauer alkylamide 11 and Bauer ketone 23 were previously found to be partially responsible forEchinacea angustifolia anti-inflammatory properties. This study further tested their importance using the inhibition of prostaglandin E2 (PGE2) and nitric oxide (NO) production by RAW264.7 mouse macrophages in the absence and presence of lipopolysaccharide (LPS) and E. angustifolia extracts, phytochemical enriched fractions, or pure synthesized standards. Molecular targets were probed using microarray, qRT-PCR, Western blot, and enzyme assays. Fractions with these phytochemicals were more potent inhibitors of LPS-induced PGE2 production than E. angustifolia extracts. Microarray did not detect changes in transcripts with ...


Inhibition Of Lentivirus Replication By Aqueous Extracts Of Prunella Vulgaris, Melinda A. Brindley, Mark P. Widrlechner, Joe-Ann McCoy, Patricia A. Murphy, Catherine C. Hauck, Ludmila Rizshsky, Basil J. Nikolau, Wendy Maury 2016 University of Iowa

Inhibition Of Lentivirus Replication By Aqueous Extracts Of Prunella Vulgaris, Melinda A. Brindley, Mark P. Widrlechner, Joe-Ann Mccoy, Patricia A. Murphy, Catherine C. Hauck, Ludmila Rizshsky, Basil J. Nikolau, Wendy Maury

Basil Nikolau

Background
Various members of the mint family have been used historically in Chinese and Native American medicine. Many of these same family members, including Prunella vulgaris, have been reported to have anti-viral activities. To further characterize the anti-lentiviral activities of P. vulgaris, water and ethanol extractions were tested for their ability to inhibit equine infectious anemia virus (EIAV) replication.

Results
Aqueous extracts contained more anti-viral activity than did ethanol extracts, displaying potent anti-lentiviral activity against virus in cell lines as well as in primary cell cultures with little to no cellular cytotoxicity. Time-of-addition studies demonstrated that the extracts were effective ...


Identification Of Light-Independent Inhibition Of Human Immunodeficiency Virus-1 Infection Through Bioguided Fractionation Of Hypericum Perforatum, Wendy Maury, Jason P. Price, Melinda A. Brindley, ChoonSeok Oh, Jeffrey D. Neighbors, David F. Wiemer, Nickolas Wills, Susan L. Carpenter, Catherine C. Hauck, Patricia A. Murphy, Mark P. Widrlechner, Kathleen Delate, Ganesh Kumar, George A. Kraus, Ludmila Rizshsky, Basil J. Nikolau 2016 University of Iowa

Identification Of Light-Independent Inhibition Of Human Immunodeficiency Virus-1 Infection Through Bioguided Fractionation Of Hypericum Perforatum, Wendy Maury, Jason P. Price, Melinda A. Brindley, Choonseok Oh, Jeffrey D. Neighbors, David F. Wiemer, Nickolas Wills, Susan L. Carpenter, Catherine C. Hauck, Patricia A. Murphy, Mark P. Widrlechner, Kathleen Delate, Ganesh Kumar, George A. Kraus, Ludmila Rizshsky, Basil J. Nikolau

Basil Nikolau

Background
Light-dependent activities against enveloped viruses in St. John's Wort (Hypericum perforatum) extracts have been extensively studied. In contrast, light-independent antiviral activity from this species has not been investigated.

Results
Here, we identify the light-independent inhibition of human immunodeficiency virus-1 (HIV-1) by highly purified fractions of chloroform extracts of H. perforatum. Both cytotoxicity and antiviral activity were evident in initial chloroform extracts, but bioassay-guided fractionation produced fractions that inhibited HIV-1 with little to no cytotoxicity. Separation of these two biological activities has not been reported for constituents responsible for the light-dependent antiviral activities. Antiviral activity was associated with more ...


Metabolomics As A Hypothesis-Generating Functional Genomics Tool For The Annotation Of Arabidopsis Thaliana Genes Of “Unknown Function”, Stephanie M. Quanbeck, Libuse Brachova, Alexis A. Campbell, Xin Guan, Ann Perera, Kun He, Seung Y. Rhee, Preeti Bais, Julie A. Dickerson, Philip M. Dixon, Gert Wohlgemuth, Oliver Fiehn, Lenore Barkan, Iris Lange, B. Markus Lange, Insuk Lee, Diego Cortes, Carolina Salazar, Joel Shuman, Vladimir Shulaev, David V. Huhman, Lloyd W. Sumner, Mary R. Roth, Ruth Welti, Hilal Ilarslan, Eve S. Wurtele, Basil J. Nikolau 2016 Iowa State University

Metabolomics As A Hypothesis-Generating Functional Genomics Tool For The Annotation Of Arabidopsis Thaliana Genes Of “Unknown Function”, Stephanie M. Quanbeck, Libuse Brachova, Alexis A. Campbell, Xin Guan, Ann Perera, Kun He, Seung Y. Rhee, Preeti Bais, Julie A. Dickerson, Philip M. Dixon, Gert Wohlgemuth, Oliver Fiehn, Lenore Barkan, Iris Lange, B. Markus Lange, Insuk Lee, Diego Cortes, Carolina Salazar, Joel Shuman, Vladimir Shulaev, David V. Huhman, Lloyd W. Sumner, Mary R. Roth, Ruth Welti, Hilal Ilarslan, Eve S. Wurtele, Basil J. Nikolau

Basil Nikolau

Metabolomics is the methodology that identifies and measures global pools of small molecules (of less than about 1,000 Da) of a biological sample, which are collectively called the metabolome. Metabolomics can therefore reveal the metabolic outcome of a genetic or environmental perturbation of a metabolic regulatory network, and thus provide insights into the structure and regulation of that network. Because of the chemical complexity of the metabolome and limitations associated with individual analytical platforms for determining the metabolome, it is currently difficult to capture the complete metabolome of an organism or tissue, which is in contrast to genomics and ...


Plantmetabolomics.Org: Mass Spectrometry-Based Arabidopsis Metabolomics—Database And Tools Update, Preeti Bais, Stephanie M. Moon-Quanbeck, Basil J. Nikolau, Julie A. Dickerson 2016 Iowa State University

Plantmetabolomics.Org: Mass Spectrometry-Based Arabidopsis Metabolomics—Database And Tools Update, Preeti Bais, Stephanie M. Moon-Quanbeck, Basil J. Nikolau, Julie A. Dickerson

Basil Nikolau

The PlantMetabolomics (PM) database (http://www.plantmetabolomics.org) contains comprehensive targeted and untargeted mass spectrum metabolomics data for Arabidopsis mutants across a variety of metabolomics platforms. The database allows users to generate hypotheses about the changes in metabolism for mutants with genes of unknown function. Version 2.0 of PlantMetabolomics.org currently contains data for 140 mutant lines along with the morphological data. A web-based data analysis wizard allows researchers to select preprocessing and data-mining procedures to discover differences between mutants. This community resource enables researchers to formulate models of the metabolic network of Arabidopsis and enhances the research community ...


The Role Of Pyruvate Dehydrogenase And Acetyl-Coenzyme A Synthetase In Fatty Acid Synthesis In Developing Arabidopsis Seeds, Jinshan Ke, Robert H. Behal, Stephanie L. Back, Basil J. Nikolau, Eve S. Wurtele, David J. Oliver 2016 Iowa State University

The Role Of Pyruvate Dehydrogenase And Acetyl-Coenzyme A Synthetase In Fatty Acid Synthesis In Developing Arabidopsis Seeds, Jinshan Ke, Robert H. Behal, Stephanie L. Back, Basil J. Nikolau, Eve S. Wurtele, David J. Oliver

Basil Nikolau

Acetyl-coenzyme A (acetyl-CoA) formed within the plastid is the precursor for the biosynthesis of fatty acids and, through them, a range of important biomolecules. The source of acetyl-CoA in the plastid is not known, but two enzymes are thought to be involved: acetyl-CoA synthetase and plastidic pyruvate dehydrogenase. To determine the importance of these two enzymes in synthesizing acetyl-CoA during lipid accumulation in developing Arabidopsis seeds, we isolated cDNA clones for acetyl-CoA synthetase and for the ptE1α- and ptE1β-subunits of plastidic pyruvate dehydrogenase. To our knowledge, this is the first reported acetyl-CoA synthetase sequence from a plant source. The Arabidopsis ...


Somatostatin Receptors Signal Through Efa6a-Arf6 To Activate Phospholipase D In Clonal Β-Cells, Justin A. Grodnitzky, Nasser Syed, Michael J. Kimber, Tim A. Day, Julie G. Donaldson, Walter H. Hsu 2016 Iowa State University

Somatostatin Receptors Signal Through Efa6a-Arf6 To Activate Phospholipase D In Clonal Β-Cells, Justin A. Grodnitzky, Nasser Syed, Michael J. Kimber, Tim A. Day, Julie G. Donaldson, Walter H. Hsu

Michael J. Kimber

Somatostatin (SS) is a peptide hormone that inhibits insulin secretion in β-cells by activating its Gi/o-coupled receptors. Our previous work indicated that a βγ-dimer of Gi/o coupled to SS receptors can activate phospholipase D1 (PLD1) (Cheng, H., Grodnitzky, J. A., Yibchok-anun, S., Ding, J., and Hsu, W. H. (2005) Mol. Pharmacol. 67, 2162–2172). The aim of the present study was to elucidate the mechanisms underlying SS-induced PLD activation. We demonstrated the presence of ADP-ribosylation factor Arf1 and Arf6 in clonal β-cells, HIT-T15. We also determined that the activation of PLD1 was mediated through Arf6 ...


Functional Characterization Of Recombinant Interleukin (Il)-17a/F1 In The Japanese Pufferfish (Takifugu Rubripes), Jun-ichi Hikima Dr., Koshin Mihara, Shun Maekawa, Han-Ching Wang, Takashi Aoki, Tomoya Kono, Masahiro Sakai 2016 University of Miyazaki, Faculty of Agriculture

Functional Characterization Of Recombinant Interleukin (Il)-17a/F1 In The Japanese Pufferfish (Takifugu Rubripes), Jun-Ichi Hikima Dr., Koshin Mihara, Shun Maekawa, Han-Ching Wang, Takashi Aoki, Tomoya Kono, Masahiro Sakai

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


Heterologous Expression Of A Rice Mir395 Gene In Nicotiana Tabacum Impairs Sulfate Homeostasis, Ning Yuan, Shuangrong Yuan, Zhigang Li, Dayong Li, Qian Hu, Hong Luo 2016 Clemson University

Heterologous Expression Of A Rice Mir395 Gene In Nicotiana Tabacum Impairs Sulfate Homeostasis, Ning Yuan, Shuangrong Yuan, Zhigang Li, Dayong Li, Qian Hu, Hong Luo

Publications

Sulfur participates in many important mechanisms and pathways of plant development. The most common source of sulfur in soil –SO42−– is absorbed into root tissue and distributed into aerial part through vasculature system, where it is reduced into sulfite and finally sulfide within the subcellular organs such as chloroplasts and mitochondria and used for cysteine and methionine biosynthesis. MicroRNAs are involved in many regulation pathways by repressing the expression of their target genes. MiR395 family in Arabidopsis thaliana has been reported to be an important regulator involved in sulfate transport and assimilation, and a high-affinity sulphate transporter and ...


Ptpro Represses Erbb2-Driven Breast Oncogenesis By Dephosphorylation And Endosomal Internalization Of Erbb2., H Dong, L Ma, J Gan, W Lin, Rakesh Kumar, +9 additional authors 2016 George Washington University

Ptpro Represses Erbb2-Driven Breast Oncogenesis By Dephosphorylation And Endosomal Internalization Of Erbb2., H Dong, L Ma, J Gan, W Lin, Rakesh Kumar, +9 Additional Authors

Biochemistry and Molecular Medicine Faculty Publications

The plasma membrane-associated tyrosine phosphatase PTPRO is frequently transcriptionally repressed in cancers and signifies poor prognosis of breast cancer patients. In this study, deletion of Ptpro inMMTV-Erbb2 transgenic mice dramatically shortened the mammary tumor latency and accelerated tumor growth due to loss of Ptpro within the breast cancer cells but not in surrounding tissue as confirmed by hetero-transplantation studies. Both in vitro and in vivo data demonstrated that the phosphatase activity was required for the inactivation of ERBB2 and its downstream signaling. PTPRO regulated the phosphorylation status of ERBB2 at Y1248. Co-immunoprecipitation and proximity ligation assay (Duolink) indicated that ...


Leveraging The Plant Biotechnology Toolbox For Aquaculture: Production Of Protein Therapeutants For Promoting Fish Immune Health, Lana L. Elkins 2016 Arkansas State University - Main Campus

Leveraging The Plant Biotechnology Toolbox For Aquaculture: Production Of Protein Therapeutants For Promoting Fish Immune Health, Lana L. Elkins

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


Small-Angle X-Ray Scattering Studies Of The Oligomeric State And Quaternary Structure Of The Trifunctional Proline Utilization A (Puta) Flavoprotein From Escherichia Coli, Ranjan K. Singh, John D. Larson, Weidong Zhu, Robert P. Rambo, Greg L. Hura, Donald F. Becker, John J. Tanner 2016 University of Missouri-Columbia

Small-Angle X-Ray Scattering Studies Of The Oligomeric State And Quaternary Structure Of The Trifunctional Proline Utilization A (Puta) Flavoprotein From Escherichia Coli, Ranjan K. Singh, John D. Larson, Weidong Zhu, Robert P. Rambo, Greg L. Hura, Donald F. Becker, John J. Tanner

Biochemistry -- Faculty Publications

Background: Trifunctional proline utilization A (PutA) proteins are multifunctional flavoproteins that catalyze two reactions and repress transcription of the put regulon.

Results: PutA from Escherichia coli is a V-shaped dimer, with the DNA-binding domain mediating dimerization.

Conclusion: Oligomeric state and quaternary structures are not conserved by PutAs.

Significance: The first three-dimensional structural information for any trifunctional PutA is reported.


Isothermal Titration Calorimetry Uncovers Substrate Promiscuity Of Bicupin Oxalate Oxidase From Ceriporiopsis Subvermispora, Hassan Rana, Patricia Moussatche, Lis Souza Rocha, Ellen W. Moomaw 2016 Kennesaw State University

Isothermal Titration Calorimetry Uncovers Substrate Promiscuity Of Bicupin Oxalate Oxidase From Ceriporiopsis Subvermispora, Hassan Rana, Patricia Moussatche, Lis Souza Rocha, Ellen W. Moomaw

Ellen Moomaw

Isothermal titration calorimetry (ITC) may be used to determine the kinetic parameters of enzymecatalyzed reactions when neither products nor reactants are spectrophotometrically visible and when the reaction products are unknown. We report here the use of the multiple injection method of ITC to characterize the catalytic properties of oxalate oxidase (OxOx) from Ceriporiopsis subvermispora (CsOxOx), a manganese dependent enzyme that catalyzes the oxygen-dependent oxidation of oxalate to carbon dioxide in a reaction coupled with the formation of hydrogen peroxide. CsOxOx is the first bicupin enzyme identified that catalyzes this reaction. The multiple injection ITC method of measuring OxOx activity involves ...


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