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Monitoring Of Pseudomonas Aeruginosa Toxins Via Miniaturized Electrochemical Assemblies, Thaddaeus Webster 2015 Northeastern University

Monitoring Of Pseudomonas Aeruginosa Toxins Via Miniaturized Electrochemical Assemblies, Thaddaeus Webster

Chemical Engineering Dissertations

The production of pyocyanin from the opportunistic pathogen Pseudomonas aeruginosa was probed using a variety of miniaturized electrochemical systems. Goal 1 used disposable screen printed carbon electrodes to measure pyocyanin in medically relevant samples showing for the first time that pyocyanin can be detected at medically relevant concentrations (1-100 µM) without sample processing. Goal 2 coupled these same electrodes with Polydimethylsiloxane growth chambers to expose P. aeruginosa biofilms to varying concentrations of colistin sulphate. A reduction in electrochemical signal from pyocyanin, of approximately 80% when exposed to 100 mg/L colistin sulphate, highlights this molecules relation to biofilm health. Goal ...


Cellular Localization And Metabolomic Analysis Of The Arabidopsis Thaliana Major Intrinsic Protein Nip2;1: A Root-Specific Lactic Acid Transporter Induced In Response To Hypoxic Stress, Taylor K. Fuller 2015 University of Tennessee - Knoxville

Cellular Localization And Metabolomic Analysis Of The Arabidopsis Thaliana Major Intrinsic Protein Nip2;1: A Root-Specific Lactic Acid Transporter Induced In Response To Hypoxic Stress, Taylor K. Fuller

University of Tennessee Honors Thesis Projects

No abstract provided.


Chronic Inflammation As A Result Of Hepatitis C Virus Infection: A Review Of The Literature, Samantha L. Lane 2015 DePaul University

Chronic Inflammation As A Result Of Hepatitis C Virus Infection: A Review Of The Literature, Samantha L. Lane

DePaul Discoveries

Approximately 170 million people are infected with Hepatitis C virus (HCV) worldwide5,6. It is estimated that roughly 80% of those infected suffer from persistent infection with the virus; this persistence of infection is progressive, and over time can lead to fibrosis, cirrhosis, and hepatocellular carcinoma7. Chronic inflammation and apoptotic deregulation are both hallmarks of chronic HCV infection, and many molecular pathways are initiated in both the innate and adaptive immune responses during infection with this viral pathogen. The aim of this review was to survey some of the major molecular mechanisms responsible for the induction of chronic ...


Pharmacology Of Organic Cation Transporters: Focus On Structure-Function Relationships In Oct3 (Slc22a3), Dan C. Li 2015 Washington University in St Louis

Pharmacology Of Organic Cation Transporters: Focus On Structure-Function Relationships In Oct3 (Slc22a3), Dan C. Li

Undergraduate Theses—Unrestricted

Organic Cation Transporters (OCTs) are polyspecific, facilitative transporters that play major roles in metabolite and drug clearance. OCTs are promising drug targets and elucidating their mechanisms of substrate recognition is crucial for rational drug design. OCT-mediated transport of polyvalent cations remains unexplored. OCT-expressing Xenopus laevis oocytes were used to assess transport of polyamines, ubiquitous polyvalent cations of broad physiological import, but for which transport mechanisms are unknown. Dose-response analysis of radiolabelled substrate uptake revealed that polyamines are relatively low affinity, but high turnover substrates for OCTs compared to model substrate methyl-4-phenylpyridinium (MPP+). Polyamine analogs of varying hydrophobic character were screened ...


Reverse Gyrase Is Not Necessary For Survival Of Hyperthermophilic Archaeon Pyrococcus Furiosus, Farshid Taghizadeh, Michael S. Bartlett 2015 Portland State University

Reverse Gyrase Is Not Necessary For Survival Of Hyperthermophilic Archaeon Pyrococcus Furiosus, Farshid Taghizadeh, Michael S. Bartlett

Student Research Symposium

Reverse gyrase is the only known topoisomerase enzyme with positive supercoiling activity on covalently-closed DNA. This positive supercoiling is required to prevent DNA from denaturation at high temperatures. The gene that codes for this protein is present in all hyperthermophiles and absent from all mesophilic and thermophilic genomes, suggesting that this enzyme is the only hyperthermophile-specific protein. To investigate if this protein is vital for the cells, we knocked out its gene from the genome of living organism Pyrococcus furiosus. Pyrococcus furiosus is a hyperthermophilic archaeon that grows between 70°C to 103°C with an optimum growth temperature of ...


Fishing For A Sustainable Future: Aquaponics As A Method Of Food Production, Richard Ramsundar 2015 Fordham University

Fishing For A Sustainable Future: Aquaponics As A Method Of Food Production, Richard Ramsundar

Student Theses 2015-Present

This thesis compares and explains the advantages aquaponics farming has over modern industrial intensive farming. Through a comparison natural capital usage, conservation, recycling and cost, the thesis advocates for the expansion of aquaponics usage in urban settings. The thesis also explains the history of intensive farming and aquaponics in America, the science of how aquaponics operates, the economic and environmental costs of modern intensive farming versus aquaponics farming, and the social implications of aquaponics. Lastly, I propose a policy that reallocates farm subsidies by modifying the Farm Bill. Then I propose policies that support creating a new standard of farm ...


Cell And Molecular Biology: What We Know & How We Found Out (Sample Chapter), Gerald Bergtrom 2015 University of Wisconsin - Milwaukee

Cell And Molecular Biology: What We Know & How We Found Out (Sample Chapter), Gerald Bergtrom

Cell and Molecular Biology iText

No abstract provided.


Cell And Molecular Biology: What We Know & How We Found Out (Basic Itext), Gerald Bergtrom 2015 University of Wisconsin - Milwaukee

Cell And Molecular Biology: What We Know & How We Found Out (Basic Itext), Gerald Bergtrom

Cell and Molecular Biology iText

No abstract provided.


Cell And Molecular Biology: What We Know & How We Found Out (Annotated Itext), Gerald Bergtrom 2015 University of Wisconsin - Milwaukee

Cell And Molecular Biology: What We Know & How We Found Out (Annotated Itext), Gerald Bergtrom

Cell and Molecular Biology iText

No abstract provided.


Characterization Of A Novel Clade Of Transporters In Phytophthora, Stephanie Padula, Paul F. Morris Dr, Howard Casey Cromwell Dr., Menaka Ariyaratne, Andrew Wagner 2015 Bowling Green State University - Main Campus

Characterization Of A Novel Clade Of Transporters In Phytophthora, Stephanie Padula, Paul F. Morris Dr, Howard Casey Cromwell Dr., Menaka Ariyaratne, Andrew Wagner

Honors Projects

The oomycete Phytophthora parasitica has a worldwide distribution and is an economically important pathogen of more than 100 species4. RNA-seq analysis showed that one gene, PPTG_16698 has the 5th highest level of expression of all transport proteins in the zoospore stage, and is highly conserved throughout Phytophthora species. This project attempts to characterize the important biological role that PPTG_16698 plays in P. parasitica and other oomycetes. Three strategies have been implemented to accomplish this goal: growth analysis by heterologous expression in yeast, metabolite analysis in yeast, and construction of a GFP fusion protein to enable localization of the ...


Efficient In Vitro Development Of Photoreceptors From Human Pluripotent Stem Cells, Joseph C. Reynolds 2015 Dominican University of California

Efficient In Vitro Development Of Photoreceptors From Human Pluripotent Stem Cells, Joseph C. Reynolds

Master's Theses and Capstone Projects

Degeneration of the rod and cone photoreceptors in the human retina is among the most common causes of blindness. Replacing these damaged photoreceptors may help to restore vision. Repairing the damaged retina relies on the insertion of new, healthy cells. Embryonic stem (ES) cells and induced pluripotent stem (iPS) cells are two possible sources of photoreceptors to restore vision. Previous data shows that human ES cells and iPS cells can be differentiated into photoreceptors and transplanted into the eye to restore some vision. However, this process is inefficient, and costly. Here, we show a new method for inducing photoreceptor production ...


Investigations Into The Molecular Mechanisms Of Bacterial Pathogen-Host Interactions: Construction Of A Dual Plasmid System For Incorporation Of Unnatural Amino Acids Into Pseudomonas Syringae Pv. Tomato Dc3000, Scotty D. Raber 2015 University of Nebraska-Lincoln

Investigations Into The Molecular Mechanisms Of Bacterial Pathogen-Host Interactions: Construction Of A Dual Plasmid System For Incorporation Of Unnatural Amino Acids Into Pseudomonas Syringae Pv. Tomato Dc3000, Scotty D. Raber

Student Research Projects, Dissertations, and Theses - Chemistry Department

A dual plasmid system for the incorporation of unnatural amino acids into plant pathogen, Pseudomonas syringae pv. tomato DC3000, has been designed. This invention is expected to allow (a) mutations of proteins synthesized by the bacterium, P. syringae pv. tomato DC3000, that can capture molecular targets, especially for such modified proteins secreted by the phytopathogen into the host plant cells of A. thaliana and S. lycopersicum, (b) expression of biological probes in the bacterial species to monitor changes in redox, nutritional, and other small molecule states over pre-, post- and in situ disease stages, and (c) secretion of such biological ...


The Characterization Of Amyloid Fibrils And Novel Synthetic Heparin-Binding Peptides Binding To Cell Surfaces, Nicole Marie Hackenbrack 2015 University of Tennessee, Knoxville

The Characterization Of Amyloid Fibrils And Novel Synthetic Heparin-Binding Peptides Binding To Cell Surfaces, Nicole Marie Hackenbrack

University of Tennessee Honors Thesis Projects

No abstract provided.


The Cancer Genome Atlas (Tcga): Breast And Ovarian Cancers, Laura Ann Riccio 2015 University of Rhode Island

The Cancer Genome Atlas (Tcga): Breast And Ovarian Cancers, Laura Ann Riccio

Senior Honors Projects

The field of genomics originated in the 1970’s starting with the sequencing of small organisms’ genomes such as the bacterium, Haemophilus influenzae, and the yeast, Saccharomyces cerevisiae. A genome is the complete ordered sequence of DNA bases (A, C, G, and T) comprising all of the protein- and RNA-coding genes, as well as all of the regulatory sequences necessary for the construction of an organism. Over time, scientists sequenced the genomes of larger and more complex organisms, eventually leading to the sequencing of the human genome. The Human Genome Project (HGP) was initiated in 1990 and took over ten ...


Revisiting The Stoichiometry And Binding Sites Of Pai-1 In Complex With Vitronectin: Vn A Potential Mediator Of Pai-1:Pai-1 Interactions?, Sierra M. Sorter 2015 University of Tennessee - Knoxville

Revisiting The Stoichiometry And Binding Sites Of Pai-1 In Complex With Vitronectin: Vn A Potential Mediator Of Pai-1:Pai-1 Interactions?, Sierra M. Sorter

University of Tennessee Honors Thesis Projects

No abstract provided.


Discovery Of Unknown Modifications In Recombinant Monoclonal Antibodies, Christopher Michael Chumsae 2015 Northeastern University

Discovery Of Unknown Modifications In Recombinant Monoclonal Antibodies, Christopher Michael Chumsae

Chemistry Dissertations

Recombinant monoclonal antibodies have become one of the most important classes of biotherapeutics today. Due to their complexity, they must be produced by cellular expression. They are subject to various posttranslational modifications during manufacture, formulation and storage. Occasionally, the antibody may encounter a reactive molecule which in turn, results in a new variant. The application of modern analytical strategies including charge-based approaches and mass spectrometry in a defined workflow can effectively elucidate the presence of new species and help to understand the chemistry and underlying root cause. This dissertation will discuss the discovery of three novel chemical modifications in a ...


A Two-Component Regulatory System Associated With Scytonemin Biosynthesis In Nostoc Punctiforme Atcc 29133, Jacob P. Janssen 2015 Indiana University - Purdue University Fort Wayne

A Two-Component Regulatory System Associated With Scytonemin Biosynthesis In Nostoc Punctiforme Atcc 29133, Jacob P. Janssen

Masters' Theses

As phototrophic bacteria, cyanobacteria are continually exposed to ultraviolet radiation as they harvest solar energy. In particular, long-wavelength ultraviolet radiation (UVA) damages living cells by releasing reactive oxygen species. To mitigate damage to the cell, some cyanobacteria produce a UVA-absorbing pigment in the extracellular sheath, known as scytonemin. Scytonemin is a heterocyclic, dimeric molecule that is only produced upon induction by UVA. In Nostoc punctiforme ATCC 29133, it is hypothesized that scytonemin is regulated by the two component regulatory system (TCRS) of NpF1277 (sensor kinase) and NpF1278 (response regulator). Gene expression of the TCRS was studied after exposure to UVA ...


Developing An Application For Evolutionary Search For Computational Models Of Cellular Development, Nicolas Scott Cornia 2015 Boise State University

Developing An Application For Evolutionary Search For Computational Models Of Cellular Development, Nicolas Scott Cornia

Boise State University Theses and Dissertations

VPEvolve is a free and open source application that utilizes a Visual Programming Environment (VPE) for the setup of the Genetic Algorithm (GA), for optimization of computational models. Specifically, the User Interface uses connected glyphs to represent the genetic operators of mutation, reproduction, fitness and selection. These glyphs give the user an intuitive way to set the parameters for the GA, and better visualization of the population's flow through these operators.

VPEvolve is currently being developed alongside research being done in Biocomputing to create models of cellular regeneration based on the regenerative properties of Planaria or flatworms. Since these ...


Integrating Motif, Dna Accessibility And Gene Expression Data To Build Regulatory Maps In An Organism, Charles Blatti, Majid Kazemian, Scot A. Wolfe, Michael H. Brodsky, Saurabh Sinha 2015 University of Illinois at Urbana-Champaign

Integrating Motif, Dna Accessibility And Gene Expression Data To Build Regulatory Maps In An Organism, Charles Blatti, Majid Kazemian, Scot A. Wolfe, Michael H. Brodsky, Saurabh Sinha

Molecular, Cell and Cancer Biology Publications

Characterization of cell type specific regulatory networks and elements is a major challenge in genomics, and emerging strategies frequently employ high-throughput genome-wide assays of transcription factor (TF) to DNA binding, histone modifications or chromatin state. However, these experiments remain too difficult/expensive for many laboratories to apply comprehensively to their system of interest. Here, we explore the potential of elucidating regulatory systems in varied cell types using computational techniques that rely on only data of gene expression, low-resolution chromatin accessibility, and TF-DNA binding specificities ('motifs'). We show that static computational motif scans overlaid with chromatin accessibility data reasonably approximate experimentally ...


Inhibition Of Breast Cancer Cell Proliferation By Diarylthiourea Analog, Sl1-18, Maryam Fallatah 2015 Dominican University of California

Inhibition Of Breast Cancer Cell Proliferation By Diarylthiourea Analog, Sl1-18, Maryam Fallatah

Scholarly and Creative Works Conference

Breast cancer is the most common malignancy that occurs in women in the US and has the second highest cancer-associated mortality rate. Depending on the expression of estrogen receptor α (ERα), breast cancer can be classified as ER positive or negative. Current drugs used to treat ER+ cancers include selective estrogen receptor modulators (SERMs) and selective estrogen receptor down-regulators (SERDs). Unfortunately, prolonged exposure to these drugs often leads to the development of acquired resistance. Consequently, there is a great need to develop alternative therapeutic options. Flexible heteroarotinoids (Flex-Hets) are groups of compounds that are derived from retinoids. Flex-Hets have been ...


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