"Fun" And Correlations To "Happiness", 2017 University of Southern California
"Fun" And Correlations To "Happiness", Gabriel Leiner
Dietary Weight Loss Advice In Us Health Magazines And Its Relation To Ancestral Diet, 2016 Rockhurst University
Dietary Weight Loss Advice In Us Health Magazines And Its Relation To Ancestral Diet, Risa J. Stein, Marium A. Choudhry, Thomas J. Murray Jr.
Journal of Evolution and Health
As rates of overweight and obesity have risen in the US, the public has sought effective strategies for weight loss through dietary modification. A proliferation of processed foods and changing governmental nutrition guidelines have both impacted dietary intake patterns. While physicians are considered respectable sources of weight-loss information, increasingly the public has turned to the media, particularly magazines, for weight loss advice. This study investigated the dietary recommendations found in the five leading US health-related magazines and compared those recommendations to ancestral diets. With a couple notable exceptions, leading health magazines present consistent recommendations for dietary modifications to promote weight ...
Quantifying Greenhouse Gas Flux Of Restored Vs. Unrestored Wetlands: A Case Study At Prairie Wolf Slough, Matthew Connors, Beth Lawrence
Wetlands provide ecological services such as cleansing the water supply, sequestering carbon, and providing habitat for wildlife, however wetland restoration often alters the greenhouse gas flux of the site. Our study aims to investigate the effects of wetland restoration on greenhouse gas flux at Prairie Wolf Slough. We did this by comparing greenhouse gas flux on matching hydric soil series from the restored wetland with an adjacent abandoned agricultural field. We measured known controls of greenhouse gas flux such as soil moisture and soil temperature. We found that there was no detectable methane and nitrous oxide flux at either site ...
Bauer Ketones 23 And 24 From Echinacea Paradoxa Var. Paradoxa Inhibit Lipopolysaccharide-Induced Nitric Oxide, Prostaglandin E2 And Cytokines In Raw264.7 Mouse Macrophages, Xiaozhu Zhang, Ludmila Rizshsky, Catherine C. Hauck, Luping Qu, Mark P. Widrlechner, Basil J. Nikolau, Patricia A. Murphy, Diane F. Birt
Among the nine Echinacea species, E. purpurea, E. angustifolia and E. pallida, have been widely used to treat the common cold, flu and other infections. In this study, ethanol extracts of these three Echinaceaspecies and E. paradoxa, including its typical variety, E. paradoxa var. paradoxa, were screened in lipopolysaccharide (LPS)-stimulated macrophage cells to assess potential anti-inflammatory activity. E. paradoxa var. paradoxa, rich in polyenes/polyacetylenes, was an especially efficient inhibitor of LPS-induced production of nitric oxide (NO), prostaglandin E2 (PGE2), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) by 46%, 32%, 53% and 26%, respectively, when tested at 20 μg ...
Enrichment Of Echinacea Angustifolia With Bauer Alkylamide 11 And Bauer Ketone 23 Increased Anti-Inflammatory Potential Through Interference With Cox-2 Enzyme Activity, Carlie A. Lalone, Nan Huang, Ludmila Rizshsky, Man-Yu Yum, Navrozedeep Singh, Cathy Hauck, Basil J. Nikolau, Eve S. Wurtele, Marian L. Kohut, Patricia A. Murphy, Diane F. Birt
Bauer alkylamide 11 and Bauer ketone 23 were previously found to be partially responsible forEchinacea angustifolia anti-inflammatory properties. This study further tested their importance using the inhibition of prostaglandin E2 (PGE2) and nitric oxide (NO) production by RAW264.7 mouse macrophages in the absence and presence of lipopolysaccharide (LPS) and E. angustifolia extracts, phytochemical enriched fractions, or pure synthesized standards. Molecular targets were probed using microarray, qRT-PCR, Western blot, and enzyme assays. Fractions with these phytochemicals were more potent inhibitors of LPS-induced PGE2 production than E. angustifolia extracts. Microarray did not detect changes in transcripts with ...
Inhibition Of Lentivirus Replication By Aqueous Extracts Of Prunella Vulgaris, 2016 University of Iowa
Inhibition Of Lentivirus Replication By Aqueous Extracts Of Prunella Vulgaris, Melinda A. Brindley, Mark P. Widrlechner, Joe-Ann Mccoy, Patricia A. Murphy, Catherine C. Hauck, Ludmila Rizshsky, Basil J. Nikolau, Wendy Maury
Various members of the mint family have been used historically in Chinese and Native American medicine. Many of these same family members, including Prunella vulgaris, have been reported to have anti-viral activities. To further characterize the anti-lentiviral activities of P. vulgaris, water and ethanol extractions were tested for their ability to inhibit equine infectious anemia virus (EIAV) replication.
Aqueous extracts contained more anti-viral activity than did ethanol extracts, displaying potent anti-lentiviral activity against virus in cell lines as well as in primary cell cultures with little to no cellular cytotoxicity. Time-of-addition studies demonstrated that the extracts were effective ...
Identification Of Light-Independent Inhibition Of Human Immunodeficiency Virus-1 Infection Through Bioguided Fractionation Of Hypericum Perforatum, Wendy Maury, Jason P. Price, Melinda A. Brindley, Choonseok Oh, Jeffrey D. Neighbors, David F. Wiemer, Nickolas Wills, Susan L. Carpenter, Catherine C. Hauck, Patricia A. Murphy, Mark P. Widrlechner, Kathleen Delate, Ganesh Kumar, George A. Kraus, Ludmila Rizshsky, Basil J. Nikolau
Light-dependent activities against enveloped viruses in St. John's Wort (Hypericum perforatum) extracts have been extensively studied. In contrast, light-independent antiviral activity from this species has not been investigated.
Here, we identify the light-independent inhibition of human immunodeficiency virus-1 (HIV-1) by highly purified fractions of chloroform extracts of H. perforatum. Both cytotoxicity and antiviral activity were evident in initial chloroform extracts, but bioassay-guided fractionation produced fractions that inhibited HIV-1 with little to no cytotoxicity. Separation of these two biological activities has not been reported for constituents responsible for the light-dependent antiviral activities. Antiviral activity was associated with more ...
Metabolomics As A Hypothesis-Generating Functional Genomics Tool For The Annotation Of Arabidopsis Thaliana Genes Of “Unknown Function”, Stephanie M. Quanbeck, Libuse Brachova, Alexis A. Campbell, Xin Guan, Ann Perera, Kun He, Seung Y. Rhee, Preeti Bais, Julie A. Dickerson, Philip M. Dixon, Gert Wohlgemuth, Oliver Fiehn, Lenore Barkan, Iris Lange, B. Markus Lange, Insuk Lee, Diego Cortes, Carolina Salazar, Joel Shuman, Vladimir Shulaev, David V. Huhman, Lloyd W. Sumner, Mary R. Roth, Ruth Welti, Hilal Ilarslan, Eve S. Wurtele, Basil J. Nikolau
Metabolomics is the methodology that identifies and measures global pools of small molecules (of less than about 1,000 Da) of a biological sample, which are collectively called the metabolome. Metabolomics can therefore reveal the metabolic outcome of a genetic or environmental perturbation of a metabolic regulatory network, and thus provide insights into the structure and regulation of that network. Because of the chemical complexity of the metabolome and limitations associated with individual analytical platforms for determining the metabolome, it is currently difficult to capture the complete metabolome of an organism or tissue, which is in contrast to genomics and ...
Plantmetabolomics.Org: Mass Spectrometry-Based Arabidopsis Metabolomics—Database And Tools Update, 2016 Iowa State University
Plantmetabolomics.Org: Mass Spectrometry-Based Arabidopsis Metabolomics—Database And Tools Update, Preeti Bais, Stephanie M. Moon-Quanbeck, Basil J. Nikolau, Julie A. Dickerson
The PlantMetabolomics (PM) database (http://www.plantmetabolomics.org) contains comprehensive targeted and untargeted mass spectrum metabolomics data for Arabidopsis mutants across a variety of metabolomics platforms. The database allows users to generate hypotheses about the changes in metabolism for mutants with genes of unknown function. Version 2.0 of PlantMetabolomics.org currently contains data for 140 mutant lines along with the morphological data. A web-based data analysis wizard allows researchers to select preprocessing and data-mining procedures to discover differences between mutants. This community resource enables researchers to formulate models of the metabolic network of Arabidopsis and enhances the research community ...
The Role Of Pyruvate Dehydrogenase And Acetyl-Coenzyme A Synthetase In Fatty Acid Synthesis In Developing Arabidopsis Seeds, Jinshan Ke, Robert H. Behal, Stephanie L. Back, Basil J. Nikolau, Eve S. Wurtele, David J. Oliver
Acetyl-coenzyme A (acetyl-CoA) formed within the plastid is the precursor for the biosynthesis of fatty acids and, through them, a range of important biomolecules. The source of acetyl-CoA in the plastid is not known, but two enzymes are thought to be involved: acetyl-CoA synthetase and plastidic pyruvate dehydrogenase. To determine the importance of these two enzymes in synthesizing acetyl-CoA during lipid accumulation in developing Arabidopsis seeds, we isolated cDNA clones for acetyl-CoA synthetase and for the ptE1α- and ptE1β-subunits of plastidic pyruvate dehydrogenase. To our knowledge, this is the first reported acetyl-CoA synthetase sequence from a plant source. The Arabidopsis ...
Somatostatin Receptors Signal Through Efa6a-Arf6 To Activate Phospholipase D In Clonal Β-Cells, 2016 Iowa State University
Somatostatin Receptors Signal Through Efa6a-Arf6 To Activate Phospholipase D In Clonal Β-Cells, Justin A. Grodnitzky, Nasser Syed, Michael J. Kimber, Tim A. Day, Julie G. Donaldson, Walter H. Hsu
Michael J. Kimber
Somatostatin (SS) is a peptide hormone that inhibits insulin secretion in β-cells by activating its Gi/o-coupled receptors. Our previous work indicated that a βγ-dimer of Gi/o coupled to SS receptors can activate phospholipase D1 (PLD1) (Cheng, H., Grodnitzky, J. A., Yibchok-anun, S., Ding, J., and Hsu, W. H. (2005) Mol. Pharmacol. 67, 2162–2172). The aim of the present study was to elucidate the mechanisms underlying SS-induced PLD activation. We demonstrated the presence of ADP-ribosylation factor Arf1 and Arf6 in clonal β-cells, HIT-T15. We also determined that the activation of PLD1 was mediated through Arf6 ...
Functional Characterization Of Recombinant Interleukin (Il)-17a/F1 In The Japanese Pufferfish (Takifugu Rubripes), 2016 University of Miyazaki, Faculty of Agriculture
Functional Characterization Of Recombinant Interleukin (Il)-17a/F1 In The Japanese Pufferfish (Takifugu Rubripes), Jun-Ichi Hikima Dr., Koshin Mihara, Shun Maekawa, Han-Ching Wang, Takashi Aoki, Tomoya Kono, Masahiro Sakai
2nd International Conference of Fish & Shellfish Immunology
No abstract provided.
Heterologous Expression Of A Rice Mir395 Gene In Nicotiana Tabacum Impairs Sulfate Homeostasis, 2016 Clemson University
Heterologous Expression Of A Rice Mir395 Gene In Nicotiana Tabacum Impairs Sulfate Homeostasis, Ning Yuan, Shuangrong Yuan, Zhigang Li, Dayong Li, Qian Hu, Hong Luo
Sulfur participates in many important mechanisms and pathways of plant development. The most common source of sulfur in soil –SO42−– is absorbed into root tissue and distributed into aerial part through vasculature system, where it is reduced into sulfite and finally sulfide within the subcellular organs such as chloroplasts and mitochondria and used for cysteine and methionine biosynthesis. MicroRNAs are involved in many regulation pathways by repressing the expression of their target genes. MiR395 family in Arabidopsis thaliana has been reported to be an important regulator involved in sulfate transport and assimilation, and a high-affinity sulphate transporter and ...
Ptpro Represses Erbb2-Driven Breast Oncogenesis By Dephosphorylation And Endosomal Internalization Of Erbb2., 2016 George Washington University
Ptpro Represses Erbb2-Driven Breast Oncogenesis By Dephosphorylation And Endosomal Internalization Of Erbb2., H Dong, L Ma, J Gan, W Lin, Rakesh Kumar, +9 Additional Authors
Biochemistry and Molecular Medicine Faculty Publications
The plasma membrane-associated tyrosine phosphatase PTPRO is frequently transcriptionally repressed in cancers and signifies poor prognosis of breast cancer patients. In this study, deletion of Ptpro inMMTV-Erbb2 transgenic mice dramatically shortened the mammary tumor latency and accelerated tumor growth due to loss of Ptpro within the breast cancer cells but not in surrounding tissue as confirmed by hetero-transplantation studies. Both in vitro and in vivo data demonstrated that the phosphatase activity was required for the inactivation of ERBB2 and its downstream signaling. PTPRO regulated the phosphorylation status of ERBB2 at Y1248. Co-immunoprecipitation and proximity ligation assay (Duolink) indicated that ...
Leveraging The Plant Biotechnology Toolbox For Aquaculture: Production Of Protein Therapeutants For Promoting Fish Immune Health, 2016 Arkansas State University - Main Campus
Leveraging The Plant Biotechnology Toolbox For Aquaculture: Production Of Protein Therapeutants For Promoting Fish Immune Health, Lana L. Elkins
2nd International Conference of Fish & Shellfish Immunology
No abstract provided.
Small-Angle X-Ray Scattering Studies Of The Oligomeric State And Quaternary Structure Of The Trifunctional Proline Utilization A (Puta) Flavoprotein From Escherichia Coli, 2016 University of Missouri-Columbia
Small-Angle X-Ray Scattering Studies Of The Oligomeric State And Quaternary Structure Of The Trifunctional Proline Utilization A (Puta) Flavoprotein From Escherichia Coli, Ranjan K. Singh, John D. Larson, Weidong Zhu, Robert P. Rambo, Greg L. Hura, Donald F. Becker, John J. Tanner
Biochemistry -- Faculty Publications
Background: Trifunctional proline utilization A (PutA) proteins are multifunctional flavoproteins that catalyze two reactions and repress transcription of the put regulon.
Results: PutA from Escherichia coli is a V-shaped dimer, with the DNA-binding domain mediating dimerization.
Conclusion: Oligomeric state and quaternary structures are not conserved by PutAs.
Significance: The first three-dimensional structural information for any trifunctional PutA is reported.
Isothermal Titration Calorimetry Uncovers Substrate Promiscuity Of Bicupin Oxalate Oxidase From Ceriporiopsis Subvermispora, 2016 Kennesaw State University
Isothermal Titration Calorimetry Uncovers Substrate Promiscuity Of Bicupin Oxalate Oxidase From Ceriporiopsis Subvermispora, Hassan Rana, Patricia Moussatche, Lis Souza Rocha, Ellen W. Moomaw
Isothermal titration calorimetry (ITC) may be used to determine the kinetic parameters of enzymecatalyzed reactions when neither products nor reactants are spectrophotometrically visible and when the reaction products are unknown. We report here the use of the multiple injection method of ITC to characterize the catalytic properties of oxalate oxidase (OxOx) from Ceriporiopsis subvermispora (CsOxOx), a manganese dependent enzyme that catalyzes the oxygen-dependent oxidation of oxalate to carbon dioxide in a reaction coupled with the formation of hydrogen peroxide. CsOxOx is the first bicupin enzyme identified that catalyzes this reaction. The multiple injection ITC method of measuring OxOx activity involves ...
Real-Time Kinetic Studies Of Bacillus Subtilis Oxalate Decarboxylase And Ceriporiopsis Subvermispora Oxalate Oxidase Using Luminescent Oxygen Sensor, Laura Molina, Thomas Goodall, Umar Twahir, Ellen W. Moomaw
Oxalate decarboxylase (OxDC), an enzyme of the bicupin superfamily, catalyzes the decomposition of oxalate into carbon dioxide and formate at an optimal pH of 4.3 in the presence of oxygen. However, about 0.2% of all reactions occur through an oxidase mechanism that consumes oxygen while producing two equivalents of carbon dioxide and one equivalent of hydrogen peroxide. The kinetics of oxidase activity were studied by measuring the consumption of dissolved oxygen over time using a luminescent oxygen sensor. We describe the implementation of and improvements to the oxygen consumption assay. The oxidase activity of wild type OxDC was ...
Kinetic And Spectroscopic Studies Of Bicupin Oxalate Oxidase And Putative Active Site Mutants, 2016 Kennesaw State University
Kinetic And Spectroscopic Studies Of Bicupin Oxalate Oxidase And Putative Active Site Mutants, Ellen W. Moomaw, Eric Hoffer, Patricia Moussatche, John C. Salerno
Ceriporiopsis subvermispora oxalate oxidase (CsOxOx) is the first bicupin enzyme identified that catalyzes manganese-dependent oxidation of oxalate. In previous work, we have shown that the dominant contribution to catalysis comes from the monoprotonated form of oxalate binding to a form of the enzyme in which an active site carboxylic acid residue must be unprotonated. CsOxOx shares greatest sequence homology with bicupin microbial oxalate decarboxylases (OxDC) and the 241-244DASN region of the N-terminal Mn binding domain of CsOxOx is analogous to the lid region of OxDC that has been shown to determine reaction specificity. We have prepared a series of CsOxOx ...
Protein Similarity Networks Reveal Relationships Among Sequence, Structure, And Function Within The Cupin Superfamily, 2016 Kennesaw State University
Protein Similarity Networks Reveal Relationships Among Sequence, Structure, And Function Within The Cupin Superfamily, Richard Uberto, Ellen W. Moomaw
The cupin superfamily is extremely diverse and includes catalytically inactive seed storage proteins, sugar-binding metal-independent epimerases, and metal-dependent enzymes possessing dioxygenase, decarboxylase, and other activities. Although numerous proteins of this superfamily have been structurally characterized, the functions of many of them have not been experimentally determined. We report the first use of protein similarity networks (PSNs) to visualize trends of sequence and structure in order to make functional inferences in this remarkably diverse superfamily. PSNs provide a way to visualize relatedness of structure and sequence among a given set of proteins. Structure- and sequence-based clustering of cupin members reflects functional ...