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Resting And Functional Magnetic Resonance Spectroscopy Of Glutamate In Schizophrenia At 7 Tesla, Reggie E. Taylor 2015 The University of Western Ontario

Resting And Functional Magnetic Resonance Spectroscopy Of Glutamate In Schizophrenia At 7 Tesla, Reggie E. Taylor

Electronic Thesis and Dissertation Repository

Schizophrenia is a debilitating disease that affects about 1% of the population. Current therapeutic interventions mostly target dopaminergic neurotransmission but are not effective in treating all symptoms. There is growing evidence to support involvement of glutamatergic neurotransmission, which may better account for the symptomatology of schizophrenia.

Glutamate concentrations can be measured in vivo using magnetic resonance spectroscopy (MRS). Stronger MRIs provide benefits for MRS, but they also present challenges. Simulations were designed to examine how MRI field strength influences metabolite quantification. Glutamate and its metabolic precursor, glutamine, were more reliably and independently quantified with higher MRI field strengths, showing a ...


Cellular Localization And Metabolomic Analysis Of The Arabidopsis Thaliana Major Intrinsic Protein Nip2;1: A Root-Specific Lactic Acid Transporter Induced In Response To Hypoxic Stress, Taylor K. Fuller 2015 University of Tennessee - Knoxville

Cellular Localization And Metabolomic Analysis Of The Arabidopsis Thaliana Major Intrinsic Protein Nip2;1: A Root-Specific Lactic Acid Transporter Induced In Response To Hypoxic Stress, Taylor K. Fuller

University of Tennessee Honors Thesis Projects

No abstract provided.


A Study On Garments Dyeing With Direct Dyes And Pigments In Bangladesh, Tanushree Saha, Rasheda Begum Dina 2015 Department of Wet Process Engineering,Bangladesh University of Textiles,Tejgaon,Dhaka-1208,Bangladesh

A Study On Garments Dyeing With Direct Dyes And Pigments In Bangladesh, Tanushree Saha, Rasheda Begum Dina

Innovative Research Publications IRP India

Garment dyeing has been practiced for many years, both commercially and as a home procedure. Recently, interest in this technique has increased since it makes possible to give rapidest response to consumer demand, reduces lead times and inventory. To establish a profitable garment dyeing plant, all manufacturing steps must be carefully controlled. In this paper, we have tried to give an idea about the process of using different types of dyes in garments dyeing and their fastness properties.


Fatty Acid Recovery And Identification In Mars Analogue Soil Samples, Kimberly E. Lykens 2015 STAR

Fatty Acid Recovery And Identification In Mars Analogue Soil Samples, Kimberly E. Lykens

STAR (STEM Teacher and Researcher) Program Posters

A primary goal of NASA’s Mars 2020 mission is to gather and store samples of Mars soil that could possibly be returned to Earth in a future mission for investigations into past or present life. In years leading up to a potential sample return mission on Mars and potentially other worlds, samples taken from Chile’s Atacama Desert, the most arid, biologically limited desert in the world, are valuable in developing a capacity for biosignature detection, specifically when exploring fatty acid abundance. Eighteen samples were collected from two sites in the Atacama characterized by biological soil crusts (BSC). BSCs ...


The Yeast Genome Undergoes Significant Topological Reorganization In Quiescence, Mark T. Rutledge, Mariano Russo, Jon-Matthew Belton, Job Dekker, James R. Broach 2015 Pennsylvania State College of Medicine

The Yeast Genome Undergoes Significant Topological Reorganization In Quiescence, Mark T. Rutledge, Mariano Russo, Jon-Matthew Belton, Job Dekker, James R. Broach

Program in Systems Biology Publications and Presentations

We have examined the three-dimensional organization of the yeast genome during quiescence by a chromosome capture technique as a means of understanding how genome organization changes during development. For exponentially growing cells we observe high levels of inter-centromeric interaction but otherwise a predominance of intrachromosomal interactions over interchromosomal interactions, consistent with aggregation of centromeres at the spindle pole body and compartmentalization of individual chromosomes within the nucleoplasm. Three major changes occur in the organization of the quiescent cell genome. First, intrachromosomal associations increase at longer distances in quiescence as compared to growing cells. This suggests that chromosomes undergo condensation in ...


The Role Of The Pleckstrin Homology Domain-Containing Protein Ckip-1 In Activation Of P21-Activated Kinase 1 (Pak1), Yong-Bae Kim, Yong Jae Shin, Adhiraj Roy, Jeong-Ho Kim 2015 George Washington University

The Role Of The Pleckstrin Homology Domain-Containing Protein Ckip-1 In Activation Of P21-Activated Kinase 1 (Pak1), Yong-Bae Kim, Yong Jae Shin, Adhiraj Roy, Jeong-Ho Kim

Biochemistry and Molecular Medicine Faculty Publications

Upon growth factor stimulation, PAK1 is recruited to the plasma membrane and activated by a mechanism that requires its phosphorylation at S223 by the protein kinase CK2. However, the upstream signaling molecules that regulate this phosphorylation event are not clearly defined. Here, we demonstrate a major role of the CK2α-interacting protein CKIP-1 in activation of PAK1. CK2α, CKIP-1 and PAK1 are translocated to membrane ruffles in response to the epidermal growth factor (EGF), where CKIP-1 mediates the interaction between CK2α, and PAK1 in a PI3K-dependent manner. Consistently, we observe that PAK1 mediates phosphorylation and modulation of the activity of p41-Arc ...


Methods Of Detecting Target Nucleic Acids, amyi austin 2015 SelectedWorks

Methods Of Detecting Target Nucleic Acids, Amyi Austin

amyi austin

This paper talks about the methods of measuring target nucleic acids,which includes 5 sections:1.abstract, 2.what to claim,3.background, 4. summary, 5.note


Improving Alternate Lignin Catabolite Utilization Of Ligab From Sphingobium Sp. Strain Syk-6 Through Site Directed Mutagenesis, Kevin P. Barry, Erin F. Cohn, Abraham Ngu, Erika A. Taylor 2015 Wesleyan University

Improving Alternate Lignin Catabolite Utilization Of Ligab From Sphingobium Sp. Strain Syk-6 Through Site Directed Mutagenesis, Kevin P. Barry, Erin F. Cohn, Abraham Ngu, Erika A. Taylor

Erika A. Taylor, Ph.D.

Protocatechuate 4,5-dioxygenase (LigAB) catalyzes dioxygenation of multiple lignin derived aromatic compounds—such as protocatechuate (PCA), gallate (GA) and 3-O-methyl gallate (3OMG)—with decreasing proficiency as the molecule size increases. We predicted that phenylalanine-103 of the α subunit (Phe103α) controls substrate specificity through interaction with the C5-funtionality of bound substrates, and mutagenesis would enhance GA and 3OMG catalysis. LigAB with Phe103α mutations (F103 V, F103T and F103H) displayed enhanced catalytic efficiency for dioxygenation of 3OMG, with mutants displaying 12- to 31-fold increases in View the MathML source, making these mutant enzymes more active with 3OMG than its native dioxygenase (DesZ ...


Analytical Approaches In Investigating The Kinetics Of Water-Molecule Complexes In Tropospheric Reactions, William J. Keeton 2015 Brigham Young University - Provo

Analytical Approaches In Investigating The Kinetics Of Water-Molecule Complexes In Tropospheric Reactions, William J. Keeton

All Theses and Dissertations

Ozone is a heavily monitored pollutant. Ozone is not directly emitted into the atmosphere, but rather the product of chemical reactions. Ground level ozone occurs when nitrogen oxides (NOx) and volatile organic compounds (VOCs) react with each other in the presence of sunlight. The primary precursors of ozone are anthropogenically emitted, and as a result, tropospheric ozone has cost millions of dollars in damages and has hurt the health of countless people. This dissertation is a collection of work that aims to provide insight into atmospheric reactions that result in tropospheric ozone and the instrumentation to study such reactions. While ...


Histone Deacetylase 6 (Hdac6) Is Critical For Tumor Cell Survival And Promotes The Pro-Survival Activity Of 14-3-3Ζ Viadeacetylation Of Lysines Within The14-3-3Ζ Binding Pocket, Jeffrey Benjamin Mortenson 2015 Brigham Young University - Provo

Histone Deacetylase 6 (Hdac6) Is Critical For Tumor Cell Survival And Promotes The Pro-Survival Activity Of 14-3-3Ζ Viadeacetylation Of Lysines Within The14-3-3Ζ Binding Pocket, Jeffrey Benjamin Mortenson

All Theses and Dissertations

Our understanding of non-histone acetylation as a means of cellular regulation is in its infancy. Using a mass spectrometry approach we identified acetylated lysine residues and monitored acetylation changes across the proteome as a consequence of metabolic stress (hypoxia). We observed changes in acetylation status of non-histone lysines in tumor cells. Through the use of small molecule inhibitors of histone deacetylase enzymes (HDACs) and siRNA screening identified HDAC6 as a pro-survival regulator of lysine acetylation during hypoxia. The phospho-binding protein 14-3-3ζ acts as a signaling hub controlling a network of interacting partners and oncogenic pathways. We show here that lysines ...


Viral Non-Coding Rna Inhibits Hnf4Α Expression In Hcv Associated Hepatocellular Carcinoma, Zhao Wang, Kristin Ceniccola, Liliana Florea, Bi-Dar Wang, Norman H. Lee, Ajit Kumar 2015 George Washington University

Viral Non-Coding Rna Inhibits Hnf4Α Expression In Hcv Associated Hepatocellular Carcinoma, Zhao Wang, Kristin Ceniccola, Liliana Florea, Bi-Dar Wang, Norman H. Lee, Ajit Kumar

Biochemistry and Molecular Medicine Faculty Publications

Background

Hepatitis C virus (HCV) infection is an established cause of chronic hepatitis, cirrhosis and hepatocellular carcinoma (HCC); however, it is unclear if the virus plays a direct role in the development of HCC. Hepatocyte nuclear factor 4α (HNF4α) is critical determinant of epithelial architecture and hepatic development; depletion of HNF4α is correlated with oncogenic transformation. We explored the viral role in the inhibition of HNF4α expression, and consequent induction of tumor-promoting genes in HCV infection-associated HCC.

Methods

Western blot analysis was used to monitor the changes in expression levels of oncogenic proteins in liver tissues from HCV-infected humanized mice ...


An Apache Hadoop Framework For Large-Scale Peptide Identification, Harinivesh Donepudi 2015 Western Kentucky University

An Apache Hadoop Framework For Large-Scale Peptide Identification, Harinivesh Donepudi

Masters Theses & Specialist Projects

Peptide identification is an essential step in protein identification, and Peptide Spectrum Match (PSM) data set is huge, which is a time consuming process to work on a single machine. In a typical run of the peptide identification method, PSMs are positioned by a cross correlation, a statistical score, or a likelihood that the match between the trial and hypothetical is correct and unique. This process takes a long time to execute, and there is a demand for an increase in performance to handle large peptide data sets. Development of distributed frameworks are needed to reduce the processing time, but ...


World-Class Research: Biomedical Science Beyond Experimentation, 2015 Colby College

World-Class Research: Biomedical Science Beyond Experimentation

Colby Magazine

Faculty and relationships with Maine’s big-three research labs propel Colby’s opportunities in biomedical science

Could childhood nutrition set late-life brain function?

Hitting the lab running

Ahmad wins grant for study of neurodegenerative disease


Chemical Biology Of Protein Arginine Modifications In Epigenetic Regulation, Jakob Fuhrmann, Kathleen W. Clancy, Paul R. Thompson 2015 The Scripps Research Institute

Chemical Biology Of Protein Arginine Modifications In Epigenetic Regulation, Jakob Fuhrmann, Kathleen W. Clancy, Paul R. Thompson

Thompson Lab Publications

Review article on histone citrullination (arginine deimination), histone arginine methylation, and noncanonical histone arginine modifications.


A Study Of Allylic Aminations As Catalyzed By Heterobimetallic Pd-Ti Complexes, Diana Lauren Ellis 2015 Brigham Young University

A Study Of Allylic Aminations As Catalyzed By Heterobimetallic Pd-Ti Complexes, Diana Lauren Ellis

All Theses and Dissertations

Heterobimetallic complexes present a unique approach to catalyzing challenging reactions. By having two metals in close proximity to each other, the metals are able to interact and alter their electronics in a way that simple organic ligands (carbon, nitrogen, sulfur etc.) cannot. Our studies of heterobimetallic complexes focus on a Pd–Ti complex. The complex features a dative interaction between the palladium and the titanium held together by a phosphonamide scaffold. This interaction increases the electrophilicity of the palladium and makes it a very suitable catalyst for allylic amination reactions. We have conducted extensive studies of this catalyst in allylic ...


The Effect Of Osm On Mc3t3-E1 Osteoblastic Cells In Simulated Microgravity With Radiation, Jake Goyden, Ken Tawara, Danielle Hedeen, Jeffrey S. Willey, Julia Thom Oxford, Cheryl L. Jorcyk 2015 Boise State University

The Effect Of Osm On Mc3t3-E1 Osteoblastic Cells In Simulated Microgravity With Radiation, Jake Goyden, Ken Tawara, Danielle Hedeen, Jeffrey S. Willey, Julia Thom Oxford, Cheryl L. Jorcyk

Biomolecular Research Center Publications and Presentations

Bone deterioration is a challenge in long-term spaceflight with significant connections to patients experiencing disuse bone loss. Prolonged unloading and radiation exposure, defining characteristics of space travel, have both been associated with changes in inflammatory signaling via IL-6 class cytokines in bone. While there is also evidence for perturbed IL-6 class signaling in spaceflight, there has been scant examination of the connections between microgravity, radiation, and inflammatory stimuli in bone. Our lab and others have shown that the IL-6 class cytokine oncostatin M (OSM) is an important regulator of bone remodeling. We hypothesize that simulated microgravity alters osteoblast OSM signaling ...


A Model Of How Different Biology Experts Explain Molecular And Cellular Mechanisms, Caleb M. Trujillo, Trevor R. Anderson, Nancy J. Pelaez 2015 Purdue University

A Model Of How Different Biology Experts Explain Molecular And Cellular Mechanisms, Caleb M. Trujillo, Trevor R. Anderson, Nancy J. Pelaez

PIBERG Publications

Constructing explanations is an essential skill for all science learners. The goal of this project was to model the key components of expert explanation of molecular and cellular mechanisms. As such, we asked: What is an appropriate model of the components of explanation used by biology experts to explain molecular and cellular mechanisms? Do explanations made by experts from different biology subdisciplines at a university support the validity of this model? Guided by the modeling framework of R. S. Justi and J. K. Gilbert, the validity of an initial model was tested by asking seven biologists to explain a molecular ...


Chronic Inflammation As A Result Of Hepatitis C Virus Infection: A Review Of The Literature, Samantha L. Lane 2015 DePaul University

Chronic Inflammation As A Result Of Hepatitis C Virus Infection: A Review Of The Literature, Samantha L. Lane

DePaul Discoveries

Approximately 170 million people are infected with Hepatitis C virus (HCV) worldwide5,6. It is estimated that roughly 80% of those infected suffer from persistent infection with the virus; this persistence of infection is progressive, and over time can lead to fibrosis, cirrhosis, and hepatocellular carcinoma7. Chronic inflammation and apoptotic deregulation are both hallmarks of chronic HCV infection, and many molecular pathways are initiated in both the innate and adaptive immune responses during infection with this viral pathogen. The aim of this review was to survey some of the major molecular mechanisms responsible for the induction of chronic ...


Tailor: A Computational Framework For Detecting Non-Templated Tailing Of Small Silencing Rnas, Min-Te Chou, Bo W. Han, Chiung-Po Hsiao, Phillip D. Zamore, Zhiping Weng, Jui-Hung Hung 2015 National Chiao Tung University

Tailor: A Computational Framework For Detecting Non-Templated Tailing Of Small Silencing Rnas, Min-Te Chou, Bo W. Han, Chiung-Po Hsiao, Phillip D. Zamore, Zhiping Weng, Jui-Hung Hung

GSBS Student Publications

Small silencing RNAs, including microRNAs, endogenous small interfering RNAs (endo-siRNAs) and Piwi-interacting RNAs (piRNAs), have been shown to play important roles in fine-tuning gene expression, defending virus and controlling transposons. Loss of small silencing RNAs or components in their pathways often leads to severe developmental defects, including lethality and sterility. Recently, non-templated addition of nucleotides to the 3' end, namely tailing, was found to associate with the processing and stability of small silencing RNAs. Next Generation Sequencing has made it possible to detect such modifications at nucleotide resolution in an unprecedented throughput. Unfortunately, detecting such events from millions of short ...


A Multilaboratory Comparison Of Calibration Accuracy And The Performance Of External References In Analytical Ultracentrifugation, Huaying Zhao, Kimberly A. Crowley, Craig L. Peterson, Sarah G. Swygert, Peter Schuck 2015 National Institutes of Health

A Multilaboratory Comparison Of Calibration Accuracy And The Performance Of External References In Analytical Ultracentrifugation, Huaying Zhao, Kimberly A. Crowley, Craig L. Peterson, Sarah G. Swygert, Peter Schuck

Open Access Articles

Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose of this multi-laboratory study was to establish the precision and accuracy of basic data dimensions in AUC and validate previously proposed calibration techniques. Three kits of AUC cell assemblies containing radial and temperature calibration tools and a bovine serum albumin (BSA) reference sample were shared among 67 laboratories, generating 129 comprehensive data sets. These allowed for an assessment of many parameters of instrument performance, including accuracy ...


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