Cours Enzymologie Fondamentale Et Appliquée (Niveau Master 1), Mamoudou H. Dicko, Prof.
Pr. Mamoudou H. DICKO, PhD
Td Enzymo L3s5_2016.Pdf, 2016
Td Enzymo L3s5_2016.Pdf, Mamoudou H. Dicko, Prof.
Pr. Mamoudou H. DICKO, PhD
Analysis Of Alternative Storage Conditions For Dna Recovery From Field Samples, 2016 Northwestern College - Orange City
Analysis Of Alternative Storage Conditions For Dna Recovery From Field Samples, Alison Schutt, Emily Stricklin, Britta Ten Haken, Joseph Tolsma, Laurie Furlong, Sara S. Tolsma
As ecologists increasingly employ molecular methods, they find that tried and true preservation solutions (e.g. ethanol or formalin) may not be optimal when samples are targeted for genetic analyses. Before traveling to remote sample sites, researchers need to consider which preservation methods are likely to yield the largest quantity and highest quality of DNA based on their travel times and field conditions. They also need to consider whether they will have access to preservatives at remote sites and whether those preservatives can be safely transported. To determine which preservation methods would most reliably preserve tissue for genetic analysis under ...
Impact Of Germline And Somatic Missense Variations On Drug Binding Sites., 2016 George Washington University
Impact Of Germline And Somatic Missense Variations On Drug Binding Sites., C Yan, N Pattabiraman, J Goecks, P Lam, A Nayak, Y Pan, J Torcivia-Rodriguez, A Voskanian, Q Wan, Raja Mazumder
Biochemistry and Molecular Medicine Faculty Publications
Advancements in next-generation sequencing (NGS) technologies are generating a vast amount of data. This exacerbates the current challenge of translating NGS data into actionable clinical interpretations. We have comprehensively combined germline and somatic nonsynonymous single-nucleotide variations (nsSNVs) that affect drug binding sites in order to investigate their prevalence. The integrated data thus generated in conjunction with exome or whole-genome sequencing can be used to identify patients who may not respond to a specific drug because of alterations in drug binding efficacy due to nsSNVs in the target protein's gene. To identify the nsSNVs that may affect drug binding, protein-drug ...
Sample Article.Pdf, 2016 bepress university
Sample Article.Pdf, Rachel Poole
Mmb-Gui: A Fast Morphing Method Demonstrates A Possible Ribosomal Trna Translocation Trajectory, 2016 Uppsala University
Mmb-Gui: A Fast Morphing Method Demonstrates A Possible Ribosomal Trna Translocation Trajectory, Alex Tek, Andrei A. Korostelev, Samuel Coulbourn Flores
Open Access Articles
Easy-to-use macromolecular viewers, such as UCSF Chimera, are a standard tool in structural biology. They allow rendering and performing geometric operations on large complexes, such as viruses and ribosomes. Dynamical simulation codes enable modeling of conformational changes, but may require considerable time and many CPUs. There is an unmet demand from structural and molecular biologists for software in the middle ground, which would allow visualization combined with quick and interactive modeling of conformational changes, even of large complexes. This motivates MMB-GUI. MMB uses an internal-coordinate, multiscale approach, yielding as much as a 2000-fold speedup over conventional simulation methods. We use ...
Repeated Intravenous Doses Of All-Trans-Retinoyl Β-D-Glucuronide Is Not Effective In The Treatment Of Bacterial Bronchopneumonia In Lambs But Is Devoid Of Gross And Acute Toxicity, 2016 Iowa State University
Repeated Intravenous Doses Of All-Trans-Retinoyl Β-D-Glucuronide Is Not Effective In The Treatment Of Bacterial Bronchopneumonia In Lambs But Is Devoid Of Gross And Acute Toxicity, Jack Gallup, Branka Grubor, Arun Barua, Gholamreza Mohammadi, Kim Brogden, James Olson, Mark Ackermann
Jack M Gallup
All-trans-retinoyl β-D-glucuronide, a water-soluble glucuronic acid conjugate of all-transretinoic acid, has demonstrated high biological activity and low toxicity in most in vitro and in vivo models. Since the reparative effects of retinoids on epithelium are well-known, our aim was to study the effect(s) of intravenously-administered all-trans-retinoyl β-D-glucuronide in lambs with chronic bacterial bronchopneumonia.
Two groups of lambs were inoculated intrabronchially with either pyrogen-free saline or Mannheimia haemolytica. Thirty-three days later, lambs were injected four times at five-day intervals with 2 mL of 116 mM all-trans-retinoyl β-D-glucuronide (6.0–9.3 µmol/kg or 2.86–4 ...
Autoimmunity In Picornavirus Infections, 2016 University of Nebraska-Lincoln
Autoimmunity In Picornavirus Infections, Chandirasegara Massilamany, Andreas Koenig, Jay Reddy, Sally Huber, Iwona Buskiewicz
Papers in Veterinary and Biomedical Science
Enteroviruses are small, non-enveloped, positive-sense singlestrand RNA viruses, and are ubiquitously found throughout the world. These viruses usually cause asymptomatic or mild febrile illnesses, but have a propensity to induce severe diseases including type 1 diabetes and pancreatitis, paralysis and neuroinflammatory disease, myocarditis, or hepatitis. This pathogenicity may result from induction of autoimmunity to organ-specific antigens. While enterovirus-triggered autoimmunity can arise from multiple mechanisms including antigenic mimicry and release of sequestered antigens, the recent demonstration of T cells expressing dual T cell receptors arising as a natural consequence of Theiler’s virus infection is the first demonstration of this autoimmune ...
The Role Of Oxidative Stress In The Mechanisms Of Ammonia-Induced Brain Swelling And Tolerance In The Goldfish (Carassius Auratus), 2016 Wilfrid Laurier University
The Role Of Oxidative Stress In The Mechanisms Of Ammonia-Induced Brain Swelling And Tolerance In The Goldfish (Carassius Auratus), David F. Jones Lisser Mr.
Theses and Dissertations (Comprehensive)
Toxic build-ups of ammonia can cause potentially fatal brain swelling in mammals, but such swelling is reversible in the anoxia- and ammonia-tolerant goldfish (Carassius auratus). The mechanisms of ammonia-induced brain swelling and tolerance remain elusive, but several studies have suggested a role for reactive oxygen species (ROS), which may damage proteins and lipids in the plasma membrane of astrocytes in the brain. As a result, osmotic gradients across cell membranes may be altered leading to water uptake by astrocytes and swelling. While a role for ROS has been proposed in mammals, no studies have addressed this question in teleosts, in ...
A Review Of Natural And Engineered Enzymes Involved In Bioethanol Production, 2016 University of Montana
A Review Of Natural And Engineered Enzymes Involved In Bioethanol Production, Ines Cuesta Urena
Theses, Dissertations, Professional Papers
Alternative petroleum-derived fuels, such as biofuels, is another form to decrease the dependence of non-renewable energy. The most promising alternative energy is cellulosic ethanol because of the abundance of cellulose and the overall lack of concern for the food-versus-fuel dilemma.
In order to produce ethanol from cellulosic materials, pretreatment is required to “open” the lignocellulosic matrix and make cellulose more susceptible to enzymatic degradation. Enzymatic hydrolysis of lignocellulose is an important area of research due to the absence of negative effects in downstream processes in comparison with acid hydrolysis. Both natural enzymes and engineered enzymes can be used in the ...
Fluorescein Tri-Aldehyde Promotes The Selective Detection Of Homocysteine, 2015 Portland State University
Fluorescein Tri-Aldehyde Promotes The Selective Detection Of Homocysteine, Aabha Barve, Mark Lowry, Jorge O. Escobedo, Josephrajan Thainashmuthu, Robert M. Strongin
Robert M. Strongin
Amyloid Protein Aggregation In Type-Ii Diabetes, 2015 Loyola Marymount University
Amyloid Protein Aggregation In Type-Ii Diabetes, Shannon M. Pilcher
Research & Exhibition
A main cause of Type-II Diabetes has been identified as the clumping and aggregation of certain proteins within the body. Misfolding causes these insoluble, fibrous proteins, known as amyloid proteins,, thus causing fibrous protein deposits within the body that lead to toxic effects. Islet amyloid polypeptide (IAPP) is the polypeptide known to result in protein misfolding and aggregation in the pancreas, but is present in both healthy and diabetes-affected individuals. IAPP, released by pancreatic B-cells, is secreted with insulin to maintain healthy glucose levels within the body, but cell conditions can cause IAPP to have amyloidogenic properties, thus killing such ...
The Role Of Thymine Dna Glycosylase (Tdg) And Dna Demethylation In Tgf Beta Signaling, 2015 The University of Western Ontario
The Role Of Thymine Dna Glycosylase (Tdg) And Dna Demethylation In Tgf Beta Signaling, Matthew E.R. Maitland
Electronic Thesis and Dissertation Repository
Prompted by findings that TGFβ stimulates thymine DNA glycosylase (TDG) dependent rapid DNA demethylation and activation of the CDKN2B gene, I investigated the global role of TDG and DNA demethylation in TGFβ signaling in HaCaT cells. Using dot blot analysis, I show that TGFβ treatment increases the global levels of 5-formylcytosine, an intermediate metabolite of active DNA demethylation. Characterization of genomic regions that undergo DNA demethylation and recruitment of TDG indicate that they are both frequent events, but only overlap at 11 genomic locations. I identified 440 TGFβ upregulated genes, 40 of which were bound by TDG and 169 that ...
Microsolvation Of Anions By Molecules Forming Ch∙∙X- Hydrogen Bonds, 2015 Utah State University
Microsolvation Of Anions By Molecules Forming Ch∙∙X- Hydrogen Bonds, Steve Scheiner, Binod Nepal
Chemistry and Biochemistry Faculty Publications
Various anions were surrounded by n molecules of CF3H, which was used as a prototype CH donor solvent, and the structures and energies studied by M06-2X calculations with a 6-31+G∗∗ basis set. Anions considered included the halides F-, Cl-, Br- and I-, as well as those with multiple proton acceptor sites: CN-, NO3 -, HCOO-, CH3COO-, HSO4 -, H2PO4 -, and anions with higher charges SO4 2-, HPO4 2- and PO4 3-. Well structured cages were formed and the average H-bond energy decreases steadily as the number of surrounding solvent ...
Aggregation Characterization Of Wild-Type P53 And Six Common P53 Mutants, 2015 Loyola Marymount University and Loyola Law School
Aggregation Characterization Of Wild-Type P53 And Six Common P53 Mutants, Taylor A. Arhar
P53 is a tumor suppressor protein, which functions in maintaining the cell cycle. When p53 loses its function, cells may multiply at an uncontrolled rate and form tumors. This loss of function is linked to over fifty percent of human cancers. This investigation aims to explore the possible link between p53 aggregation and tumorigenesis. There is a possibility that p53, especially in mutant form, will aggregate beyond its normal tetrameric conformation and lose its function, leading to tumor formation. Wild-type p53 and six mutants, R175H, R175C, R248Q, R248W, R273C, and R273H (six of the most common mutations found in human ...
The Mysterious Relationship Of Type 2 Diabetes And Iapp Sequences, 2015 Loyola Marymount University
The Mysterious Relationship Of Type 2 Diabetes And Iapp Sequences, Larry M. Palato, David Moffet
Research & Exhibition
The scientific community’s knowledge on the disease type 2 diabetes mellitus is lacking. There are treatments for the disease, but a cure is nowhere in sight due to the lack of understanding of the pathogenesis of this disease. Of course, there is a wide variety of research currently being preformed in the pursuit of this understanding. Two areas of interest to both my mentor I are IAPP sequences and T2DM in other mammalian species. In our research we would like to combine these two areas of research and answer the question, “what similarities are there between the IAPP sequences ...
To Drink Or Not To Drink: Purity Of West La’S Public Drinking Water, 2015 Loyola Marymount University
To Drink Or Not To Drink: Purity Of West La’S Public Drinking Water, Edward Njoo
Research & Exhibition
No abstract provided.
Characterization And Structure Of A Zn2+ And [2fe-2s]-Containing Copper Chaperone From Archaeoglobus Fulgidus, 2015 Northwestern University
Characterization And Structure Of A Zn2+ And [2fe-2s]-Containing Copper Chaperone From Archaeoglobus Fulgidus, Matthew Sazinsky, Benjamin Lemoine, Maria Orofino, Roman Davydov, Krisztina Bencze, Timothy Stemmler, Brian Hoffman, José Argüello, Amy Rosenzweig
José M. Argüello
Bacterial CopZ proteins deliver copper to P1B-type Cu+-ATPases that are homologous to the human Wilson and Menkes disease proteins. The genome of the hyperthermophile Archaeoglobus fulgidus encodes a putative CopZ copper chaperone that contains an unusual cysteine rich N-terminal domain of 130 amino acids in addition to a C-terminal copper-binding domain with a conserved CXXC motif. The N-terminal domain (CopZ-NT) is homologous to proteins found only in extremophiles and is the only such protein that is fused to a copper chaperone. Surprisingly, optical, electron paramagnetic resonance, and X-ray absorption spectroscopic data indicate the presence of a [2Fe-2S] cluster in ...
Predation On Gulls By Bald Eagles In Washington, 2015 Andrews University
Predation On Gulls By Bald Eagles In Washington, James Hayward, W. Gillett, Charles Amlaner, John Stout
No abstract provided.
Evolutionary Dynamics Of Immune-Related Genes And Pathways In Disease-Vector Mosquitoes, 2015 Imperial College London
Evolutionary Dynamics Of Immune-Related Genes And Pathways In Disease-Vector Mosquitoes, Robert Waterhouse, Evgenia Kriventseva, Stephen Meister, Zhiyong Xi, Kanwal Alvarez, Lyric Bartholomay, Carolina Barillas-Mury, Guowu Bian, Stephanie Blandin, Bruce Christensen, Yuemei Dong, Haobo Jiang, Michael Kanost, Anastasios Koutsos, Elena Levashima, Jianyong Li, Petros Ligoxygakis, Robert Maccallum, George Mayhew, Antonio Mendes, Kristin Michel, Mike Osta, Susan Paskewitz, Sang Woon Shin, Dina Vlachou, Lihui Wang, Weiqi Wei, Liangbiao Zheng, Zhen Zou, David Severson, Alexander Raikhel, Fotis Kafatos, George Dimopoulos, Evgeny Zdobnov, Geoge Christophides
Mosquitoes are vectors of parasitic and viral diseases of immense importance for public health. The acquisition of the genome sequence of the yellow fever and Dengue vector, Aedes aegypti (Aa), has enabled a comparative phylogenomic analysis of the insect immune repertoire: in Aa, the malaria vector Anopheles gambiae (Ag), and the fruit fly Drosophila melanogaster (Dm). Analysis of immune signaling pathways and response modules reveals both conservative and rapidly evolving features associated with different functional gene categories and particular aspects of immune reactions. These dynamics reflect in part continuous readjustment between accommodation and rejection of pathogens and suggest how innate ...