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Cours Enzymologie Fondamentale Et Appliquée (Niveau Master 1), Mamoudou H. DICKO, Prof. 2016

Cours Enzymologie Fondamentale Et Appliquée (Niveau Master 1), Mamoudou H. Dicko, Prof.

Pr. Mamoudou H. DICKO, PhD

L'objectif principal de ce cours est de rappeler et donner aux étudiants les connaissances indispensables en enzymologie appliquée, à savoir :
- connaître la structure des enzymes, les méthodes d’extraction, de purification, de dosage et de caractérisation (électrophorèse, zymographie, etc.) des enzymes ;
- comprendre les principes fondamentaux de la cinétique chimique et la cinétique enzymatique y compris le rôle des inhibiteurs et autres effecteurs;
- comprendre les méthodes d’immobilisation des enzymes et leurs conséquences positives ou négatives dans la biocatalyse enzymatique;
- comprendre les différends domaines d’utilisation des enzymes en biochimie analytique, bio-industries, industrie agro-alimentaires, analyses bio-médicales, en diagnostic, etc.


Td Enzymo L3s5_2016.Pdf, Mamoudou H. DICKO, Prof. 2016

Td Enzymo L3s5_2016.Pdf, Mamoudou H. Dicko, Prof.

Pr. Mamoudou H. DICKO, PhD

Travaux Dirigés (TD) d'Enzymologie


Analysis Of Alternative Storage Conditions For Dna Recovery From Field Samples, Alison Schutt, Emily Stricklin, Britta Ten Haken, Joseph Tolsma, Laurie Furlong, Sara S. Tolsma 2016 Northwestern College - Orange City

Analysis Of Alternative Storage Conditions For Dna Recovery From Field Samples, Alison Schutt, Emily Stricklin, Britta Ten Haken, Joseph Tolsma, Laurie Furlong, Sara S. Tolsma

Northwestern Review

As ecologists increasingly employ molecular methods, they find that tried and true preservation solutions (e.g. ethanol or formalin) may not be optimal when samples are targeted for genetic analyses. Before traveling to remote sample sites, researchers need to consider which preservation methods are likely to yield the largest quantity and highest quality of DNA based on their travel times and field conditions. They also need to consider whether they will have access to preservatives at remote sites and whether those preservatives can be safely transported. To determine which preservation methods would most reliably preserve tissue for genetic analysis under ...


Impact Of Germline And Somatic Missense Variations On Drug Binding Sites., C Yan, N Pattabiraman, J Goecks, P Lam, A Nayak, Y Pan, J Torcivia-Rodriguez, A Voskanian, Q Wan, Raja Mazumder 2016 George Washington University

Impact Of Germline And Somatic Missense Variations On Drug Binding Sites., C Yan, N Pattabiraman, J Goecks, P Lam, A Nayak, Y Pan, J Torcivia-Rodriguez, A Voskanian, Q Wan, Raja Mazumder

Biochemistry and Molecular Medicine Faculty Publications

Advancements in next-generation sequencing (NGS) technologies are generating a vast amount of data. This exacerbates the current challenge of translating NGS data into actionable clinical interpretations. We have comprehensively combined germline and somatic nonsynonymous single-nucleotide variations (nsSNVs) that affect drug binding sites in order to investigate their prevalence. The integrated data thus generated in conjunction with exome or whole-genome sequencing can be used to identify patients who may not respond to a specific drug because of alterations in drug binding efficacy due to nsSNVs in the target protein's gene. To identify the nsSNVs that may affect drug binding, protein-drug ...


Sample Article.Pdf, Rachel Poole 2016 bepress university

Sample Article.Pdf, Rachel Poole

Rachel Poole

Testing upload


Mmb-Gui: A Fast Morphing Method Demonstrates A Possible Ribosomal Trna Translocation Trajectory, Alex Tek, Andrei A. Korostelev, Samuel Coulbourn Flores 2016 Uppsala University

Mmb-Gui: A Fast Morphing Method Demonstrates A Possible Ribosomal Trna Translocation Trajectory, Alex Tek, Andrei A. Korostelev, Samuel Coulbourn Flores

Open Access Articles

Easy-to-use macromolecular viewers, such as UCSF Chimera, are a standard tool in structural biology. They allow rendering and performing geometric operations on large complexes, such as viruses and ribosomes. Dynamical simulation codes enable modeling of conformational changes, but may require considerable time and many CPUs. There is an unmet demand from structural and molecular biologists for software in the middle ground, which would allow visualization combined with quick and interactive modeling of conformational changes, even of large complexes. This motivates MMB-GUI. MMB uses an internal-coordinate, multiscale approach, yielding as much as a 2000-fold speedup over conventional simulation methods. We use ...


Repeated Intravenous Doses Of All-Trans-Retinoyl Β-D-Glucuronide Is Not Effective In The Treatment Of Bacterial Bronchopneumonia In Lambs But Is Devoid Of Gross And Acute Toxicity, Jack Gallup, Branka Grubor, Arun Barua, Gholamreza Mohammadi, Kim Brogden, James Olson, Mark Ackermann 2016 Iowa State University

Repeated Intravenous Doses Of All-Trans-Retinoyl Β-D-Glucuronide Is Not Effective In The Treatment Of Bacterial Bronchopneumonia In Lambs But Is Devoid Of Gross And Acute Toxicity, Jack Gallup, Branka Grubor, Arun Barua, Gholamreza Mohammadi, Kim Brogden, James Olson, Mark Ackermann

Jack M Gallup

Background
All-trans-retinoyl β-D-glucuronide, a water-soluble glucuronic acid conjugate of all-transretinoic acid, has demonstrated high biological activity and low toxicity in most in vitro and in vivo models. Since the reparative effects of retinoids on epithelium are well-known, our aim was to study the effect(s) of intravenously-administered all-trans-retinoyl β-D-glucuronide in lambs with chronic bacterial bronchopneumonia.

Material/Methods
Two groups of lambs were inoculated intrabronchially with either pyrogen-free saline or Mannheimia haemolytica. Thirty-three days later, lambs were injected four times at five-day intervals with 2 mL of 116 mM all-trans-retinoyl β-D-glucuronide (6.0–9.3 µmol/kg or 2.86–4 ...


Autoimmunity In Picornavirus Infections, Chandirasegara Massilamany, Andreas Koenig, Jay Reddy, Sally Huber, Iwona Buskiewicz 2016 University of Nebraska-Lincoln

Autoimmunity In Picornavirus Infections, Chandirasegara Massilamany, Andreas Koenig, Jay Reddy, Sally Huber, Iwona Buskiewicz

Papers in Veterinary and Biomedical Science

Enteroviruses are small, non-enveloped, positive-sense singlestrand RNA viruses, and are ubiquitously found throughout the world. These viruses usually cause asymptomatic or mild febrile illnesses, but have a propensity to induce severe diseases including type 1 diabetes and pancreatitis, paralysis and neuroinflammatory disease, myocarditis, or hepatitis. This pathogenicity may result from induction of autoimmunity to organ-specific antigens. While enterovirus-triggered autoimmunity can arise from multiple mechanisms including antigenic mimicry and release of sequestered antigens, the recent demonstration of T cells expressing dual T cell receptors arising as a natural consequence of Theiler’s virus infection is the first demonstration of this autoimmune ...


The Role Of Oxidative Stress In The Mechanisms Of Ammonia-Induced Brain Swelling And Tolerance In The Goldfish (Carassius Auratus), David F. Jones Lisser Mr. 2016 Wilfrid Laurier University

The Role Of Oxidative Stress In The Mechanisms Of Ammonia-Induced Brain Swelling And Tolerance In The Goldfish (Carassius Auratus), David F. Jones Lisser Mr.

Theses and Dissertations (Comprehensive)

Toxic build-ups of ammonia can cause potentially fatal brain swelling in mammals, but such swelling is reversible in the anoxia- and ammonia-tolerant goldfish (Carassius auratus). The mechanisms of ammonia-induced brain swelling and tolerance remain elusive, but several studies have suggested a role for reactive oxygen species (ROS), which may damage proteins and lipids in the plasma membrane of astrocytes in the brain. As a result, osmotic gradients across cell membranes may be altered leading to water uptake by astrocytes and swelling. While a role for ROS has been proposed in mammals, no studies have addressed this question in teleosts, in ...


A Review Of Natural And Engineered Enzymes Involved In Bioethanol Production, Ines Cuesta Urena 2016 University of Montana

A Review Of Natural And Engineered Enzymes Involved In Bioethanol Production, Ines Cuesta Urena

Theses, Dissertations, Professional Papers

Alternative petroleum-derived fuels, such as biofuels, is another form to decrease the dependence of non-renewable energy. The most promising alternative energy is cellulosic ethanol because of the abundance of cellulose and the overall lack of concern for the food-versus-fuel dilemma.

In order to produce ethanol from cellulosic materials, pretreatment is required to “open” the lignocellulosic matrix and make cellulose more susceptible to enzymatic degradation. Enzymatic hydrolysis of lignocellulose is an important area of research due to the absence of negative effects in downstream processes in comparison with acid hydrolysis. Both natural enzymes and engineered enzymes can be used in the ...


Fluorescein Tri-Aldehyde Promotes The Selective Detection Of Homocysteine, Aabha Barve, Mark Lowry, Jorge O. Escobedo, Josephrajan Thainashmuthu, Robert M. Strongin 2015 Portland State University

Fluorescein Tri-Aldehyde Promotes The Selective Detection Of Homocysteine, Aabha Barve, Mark Lowry, Jorge O. Escobedo, Josephrajan Thainashmuthu, Robert M. Strongin

Robert M. Strongin

Elevated homocysteine levels are a well-known independent risk factor for cardiovascular disease. To date, relatively few selective fluorescent probes for homocysteine detection have been reported. The lack of sensing reagents and remaining challenges largely derive from issues of sensitivity and/or selectivity. For example, homocysteine is a structural homologue of the more abundant (ca, 20-25 fold) aminothiol cysteine, differing only by an additional methylene group side chain. Fluorescein tri-aldehyde, described herein, has been designed and synthesized as a sensitive and selective fluorophore for the detection of homocysteine in human plasma samples. It responds to analytes selectively via a photoinduced electron ...


Amyloid Protein Aggregation In Type-Ii Diabetes, Shannon M. Pilcher 2015 Loyola Marymount University

Amyloid Protein Aggregation In Type-Ii Diabetes, Shannon M. Pilcher

Research & Exhibition

A main cause of Type-II Diabetes has been identified as the clumping and aggregation of certain proteins within the body. Misfolding causes these insoluble, fibrous proteins, known as amyloid proteins,, thus causing fibrous protein deposits within the body that lead to toxic effects. Islet amyloid polypeptide (IAPP) is the polypeptide known to result in protein misfolding and aggregation in the pancreas, but is present in both healthy and diabetes-affected individuals. IAPP, released by pancreatic B-cells, is secreted with insulin to maintain healthy glucose levels within the body, but cell conditions can cause IAPP to have amyloidogenic properties, thus killing such ...


The Role Of Thymine Dna Glycosylase (Tdg) And Dna Demethylation In Tgf Beta Signaling, Matthew E.R. Maitland 2015 The University of Western Ontario

The Role Of Thymine Dna Glycosylase (Tdg) And Dna Demethylation In Tgf Beta Signaling, Matthew E.R. Maitland

Electronic Thesis and Dissertation Repository

Prompted by findings that TGFβ stimulates thymine DNA glycosylase (TDG) dependent rapid DNA demethylation and activation of the CDKN2B gene, I investigated the global role of TDG and DNA demethylation in TGFβ signaling in HaCaT cells. Using dot blot analysis, I show that TGFβ treatment increases the global levels of 5-formylcytosine, an intermediate metabolite of active DNA demethylation. Characterization of genomic regions that undergo DNA demethylation and recruitment of TDG indicate that they are both frequent events, but only overlap at 11 genomic locations. I identified 440 TGFβ upregulated genes, 40 of which were bound by TDG and 169 that ...


Microsolvation Of Anions By Molecules Forming Ch∙∙X- Hydrogen Bonds, Steve Scheiner, Binod Nepal 2015 Utah State University

Microsolvation Of Anions By Molecules Forming Ch∙∙X- Hydrogen Bonds, Steve Scheiner, Binod Nepal

Chemistry and Biochemistry Faculty Publications

Various anions were surrounded by n molecules of CF3H, which was used as a prototype CH donor solvent, and the structures and energies studied by M06-2X calculations with a 6-31+G∗∗ basis set. Anions considered included the halides F-, Cl-, Br- and I-, as well as those with multiple proton acceptor sites: CN-, NO3 -, HCOO-, CH3COO-, HSO4 -, H2PO4 -, and anions with higher charges SO4 2-, HPO4 2- and PO4 3-. Well structured cages were formed and the average H-bond energy decreases steadily as the number of surrounding solvent ...


Aggregation Characterization Of Wild-Type P53 And Six Common P53 Mutants, Taylor A. Arhar 2015 Loyola Marymount University and Loyola Law School

Aggregation Characterization Of Wild-Type P53 And Six Common P53 Mutants, Taylor A. Arhar

Honors Thesis

P53 is a tumor suppressor protein, which functions in maintaining the cell cycle. When p53 loses its function, cells may multiply at an uncontrolled rate and form tumors. This loss of function is linked to over fifty percent of human cancers. This investigation aims to explore the possible link between p53 aggregation and tumorigenesis. There is a possibility that p53, especially in mutant form, will aggregate beyond its normal tetrameric conformation and lose its function, leading to tumor formation. Wild-type p53 and six mutants, R175H, R175C, R248Q, R248W, R273C, and R273H (six of the most common mutations found in human ...


The Mysterious Relationship Of Type 2 Diabetes And Iapp Sequences, Larry M. Palato, David Moffet 2015 Loyola Marymount University

The Mysterious Relationship Of Type 2 Diabetes And Iapp Sequences, Larry M. Palato, David Moffet

Research & Exhibition

The scientific community’s knowledge on the disease type 2 diabetes mellitus is lacking. There are treatments for the disease, but a cure is nowhere in sight due to the lack of understanding of the pathogenesis of this disease. Of course, there is a wide variety of research currently being preformed in the pursuit of this understanding. Two areas of interest to both my mentor I are IAPP sequences and T2DM in other mammalian species. In our research we would like to combine these two areas of research and answer the question, “what similarities are there between the IAPP sequences ...


To Drink Or Not To Drink: Purity Of West La’S Public Drinking Water, Edward Njoo 2015 Loyola Marymount University

To Drink Or Not To Drink: Purity Of West La’S Public Drinking Water, Edward Njoo

Research & Exhibition

No abstract provided.


Characterization And Structure Of A Zn2+ And [2fe-2s]-Containing Copper Chaperone From Archaeoglobus Fulgidus, Matthew Sazinsky, Benjamin LeMoine, Maria Orofino, Roman Davydov, Krisztina Bencze, Timothy Stemmler, Brian Hoffman, José Argüello, Amy Rosenzweig 2015 Northwestern University

Characterization And Structure Of A Zn2+ And [2fe-2s]-Containing Copper Chaperone From Archaeoglobus Fulgidus, Matthew Sazinsky, Benjamin Lemoine, Maria Orofino, Roman Davydov, Krisztina Bencze, Timothy Stemmler, Brian Hoffman, José Argüello, Amy Rosenzweig

José M. Argüello

Bacterial CopZ proteins deliver copper to P1B-type Cu+-ATPases that are homologous to the human Wilson and Menkes disease proteins. The genome of the hyperthermophile Archaeoglobus fulgidus encodes a putative CopZ copper chaperone that contains an unusual cysteine rich N-terminal domain of 130 amino acids in addition to a C-terminal copper-binding domain with a conserved CXXC motif. The N-terminal domain (CopZ-NT) is homologous to proteins found only in extremophiles and is the only such protein that is fused to a copper chaperone. Surprisingly, optical, electron paramagnetic resonance, and X-ray absorption spectroscopic data indicate the presence of a [2Fe-2S] cluster in ...


Predation On Gulls By Bald Eagles In Washington, James Hayward, W. Gillett, Charles Amlaner, John Stout 2015 Andrews University

Predation On Gulls By Bald Eagles In Washington, James Hayward, W. Gillett, Charles Amlaner, John Stout

John Stout

No abstract provided.


Evolutionary Dynamics Of Immune-Related Genes And Pathways In Disease-Vector Mosquitoes, Robert Waterhouse, Evgenia Kriventseva, Stephen Meister, Zhiyong Xi, Kanwal Alvarez, Lyric Bartholomay, Carolina Barillas-Mury, Guowu Bian, Stephanie Blandin, Bruce Christensen, Yuemei Dong, Haobo Jiang, Michael Kanost, Anastasios Koutsos, Elena Levashima, Jianyong Li, Petros Ligoxygakis, Robert MacCallum, George Mayhew, Antonio Mendes, Kristin Michel, Mike Osta, Susan Paskewitz, Sang Woon Shin, Dina Vlachou, Lihui Wang, Weiqi Wei, Liangbiao Zheng, Zhen Zou, David Severson, Alexander Raikhel, Fotis Kafatos, George Dimopoulos, Evgeny Zdobnov, Geoge Christophides 2015 Imperial College London

Evolutionary Dynamics Of Immune-Related Genes And Pathways In Disease-Vector Mosquitoes, Robert Waterhouse, Evgenia Kriventseva, Stephen Meister, Zhiyong Xi, Kanwal Alvarez, Lyric Bartholomay, Carolina Barillas-Mury, Guowu Bian, Stephanie Blandin, Bruce Christensen, Yuemei Dong, Haobo Jiang, Michael Kanost, Anastasios Koutsos, Elena Levashima, Jianyong Li, Petros Ligoxygakis, Robert Maccallum, George Mayhew, Antonio Mendes, Kristin Michel, Mike Osta, Susan Paskewitz, Sang Woon Shin, Dina Vlachou, Lihui Wang, Weiqi Wei, Liangbiao Zheng, Zhen Zou, David Severson, Alexander Raikhel, Fotis Kafatos, George Dimopoulos, Evgeny Zdobnov, Geoge Christophides

Lyric Bartholomay

Mosquitoes are vectors of parasitic and viral diseases of immense importance for public health. The acquisition of the genome sequence of the yellow fever and Dengue vector, Aedes aegypti (Aa), has enabled a comparative phylogenomic analysis of the insect immune repertoire: in Aa, the malaria vector Anopheles gambiae (Ag), and the fruit fly Drosophila melanogaster (Dm). Analysis of immune signaling pathways and response modules reveals both conservative and rapidly evolving features associated with different functional gene categories and particular aspects of immune reactions. These dynamics reflect in part continuous readjustment between accommodation and rejection of pathogens and suggest how innate ...


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