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Effect Of Turmeric On The Promoter Activity Of The Cyp6a8 Gene Of Drosophila Melanogaster, Alexa Stroh 2019 University of Tennessee, Knoxville

Effect Of Turmeric On The Promoter Activity Of The Cyp6a8 Gene Of Drosophila Melanogaster, Alexa Stroh

University of Tennessee Honors Thesis Projects

No abstract provided.


The Impact Of Lncrna Dysregulation On Clinicopathology And Survival Of Breast Cancer: A Systematic Review And Meta-Analysis, Tian Tian, Meng Wang, Shuai Lin, Zhiming Dan, Kang Liu, Wenge Zhu, +several additional authors 2018 George Washington University

The Impact Of Lncrna Dysregulation On Clinicopathology And Survival Of Breast Cancer: A Systematic Review And Meta-Analysis, Tian Tian, Meng Wang, Shuai Lin, Zhiming Dan, Kang Liu, Wenge Zhu, +Several Additional Authors

Biochemistry and Molecular Medicine Faculty Publications

Dysregulation of multiple long non-coding RNAs (lncRNAs) was reported to play major roles in breast cancer (BC). Here we aimed to collect most of the relevant literature to assess the prognostic value of lncRNAs in BC. To this end, we systematically searched PubMed, Embase, Web of Science, Chinese National Knowledge Infrastructure (CNKI), and Wanfang to identify published articles on the associations of lncRNAs with clinicopathology and/or survival of BC. Via this searching, we identified 70 articles involving 9,307 BC patients and regarding 48 lncRNAs. The expression of 41 lncRNAs was related to one or more clinicopathological parameters of ...


Gq Noncanonical Roles In Translational Regulation, Brett DeMarco 2018 Duquesne University

Gq Noncanonical Roles In Translational Regulation, Brett Demarco

Electronic Theses and Dissertations

This study investigates protein nucleic acid interactions, focusing on G-quadruplex (GQ) forming DNA/RNA in human disease. GQ structures are formed in DNA/RNA, when four guanine residues form planar tetrads stabilized by Hoogsteen base pairing, that stack forming a GQ structure stabilized by potassium ions. These GQ structures are targeted by the arginine glycine-glycine (RGG) RNA-binding domain. Fragile X mental retardation protein (FMRP), a translation regulator protein implicated in the fragile X syndrome, has an RGG domain and has been previously shown to interact with neuronal GQ forming messenger RNA (mRNA). We have investigated three neuronal FMRP mRNA targets ...


Cost-Effective Paper-Based Diagnostic Using Split Proteins To Detect Yeast Infections, Zachary R. Berglund, Kevin V. Solomon, Mohit S. Verma, Moiz Rasheed, Zachary Hartley, Kevin Fitzgerald, Kok Zhi Lee, Janice Chan, Julianne Dejoie, Makayla Schacht, Alex Zavala 2018 Department of Agricultural and Biological Engineering, Purdue University

Cost-Effective Paper-Based Diagnostic Using Split Proteins To Detect Yeast Infections, Zachary R. Berglund, Kevin V. Solomon, Mohit S. Verma, Moiz Rasheed, Zachary Hartley, Kevin Fitzgerald, Kok Zhi Lee, Janice Chan, Julianne Dejoie, Makayla Schacht, Alex Zavala

The Summer Undergraduate Research Fellowship (SURF) Symposium

The common yeast infection, vulvovaginal candidiasis, affects three out of four women throughout their lifetime and can be spread to their child in the form of oral candidiasis (thrush). This disease is caused by the fungal pathogen Candida albicans, which is also a major cause of systemic candidiasis, a rarer but deadly disease with up to a 49% lethality rate. Current widely-used diagnostic methods include cell cultures, pH tests, and antibody detection, to assist effective treatment. Despite availability of various diagnostic methods, there is no inexpensive, rapid, and accurate way to detect C. albicans infection. This project aims to develop ...


Structural And Functional Characterization Of Hyper-Phosphorylated Grk5 Protein Expressed From E. Coli, Joseph M. Krampen, John Tesmer, Qiuyan Chen 2018 Purdue University

Structural And Functional Characterization Of Hyper-Phosphorylated Grk5 Protein Expressed From E. Coli, Joseph M. Krampen, John Tesmer, Qiuyan Chen

The Summer Undergraduate Research Fellowship (SURF) Symposium

G protein-coupled receptor (GPCR) kinases (GRKs) are proteins in the cell responsible for regulating GPCRs located on the cell membrane. GRKs regulate active GPCRs by phosphorylating them at certain sites which causes them to stop normal signaling on the membrane. This ultimately affects how the cell responds to its environment. GRK5 is a kinase of particular interest due to its involvement in the pathology of diseases such as cardiac failure, cancers, and diabetes. Understanding the structure and function of GRK5 is essential for discovering ways to manipulate its behavior with these diseases, but not much is known about how GRK5 ...


Water Temperature And Harmful Algal Bloom Rate, Geoff Bright, Greg Michalski, Benjamin P. Wilkins 2018 Purdue University

Water Temperature And Harmful Algal Bloom Rate, Geoff Bright, Greg Michalski, Benjamin P. Wilkins

The Summer Undergraduate Research Fellowship (SURF) Symposium

Harmful algal blooms, made up of cyanobacteria, is an increasing problem in Midwestern lakes. Nitrogen and phosphorus fertilizers used in crops such as corn and soybeans run off into streams and eventually lakes. Nitrogen and phosphorus in the form of nitrate and phosphate respectively is then used by cyanobacteria as a food source, allowing them to bloom at an alarming rate. Massive bloom events can be hazardous to both human health and the natural environment because of the release of neurotoxins, hepatotoxins and others into the air and drinking water. We set out to find if different water temperature can ...


Structural Characterization Of The Dep Domains Of P-Rex1, Samantha R. Allgood, John J.G. Tesmer, Sandeep K. Ravala 2018 Purdue University

Structural Characterization Of The Dep Domains Of P-Rex1, Samantha R. Allgood, John J.G. Tesmer, Sandeep K. Ravala

The Summer Undergraduate Research Fellowship (SURF) Symposium

P-Rex1 is a guanine nucleotide exchange factor for Rho-GTPases, which is indirectly involved in the regulation of cell migration and proliferation. It contains a tandem DH/PH domain archetypal of the Dbl family of GEFs, two DEP and two PDZ domains, and a C-terminal end with weak homology to inositol polyphosphate 4-phosphatase. P-Rex1 is regulated by both intra-domain interactions and interactions with other proteins such as G-protein beta gamma, PKA and phosphatidylinositol (3,4,5)-trisphosphate. Upregulation of P-Rex1 has been found in multiple human cancers, making it a potential target for anti-cancer drug therapies. Therefore, structural characterization of P-Rex1 ...


Engineering Bioluminescent Sensors Of Cyclic Amp To Study Opioid Signaling, Alexander L. Tesmer, Alexander R. French, Mathew Tantama 2018 Purdue University

Engineering Bioluminescent Sensors Of Cyclic Amp To Study Opioid Signaling, Alexander L. Tesmer, Alexander R. French, Mathew Tantama

The Summer Undergraduate Research Fellowship (SURF) Symposium

Opioids are small signaling molecules which bind to opioid receptors on the surface of cells. The kappa opioid receptor (KOR) is one of three major types of opioid receptors found in human neurons. When an opioid binds to a KOR, a variety of biochemical signaling pathways are activated inside the cell. Each of these pathways are associated with different physiological effects of KOR activation. The production of a small signaling molecule, cyclic adenosine monophosphate (cAMP), is known to be inhibited during KOR activation of the analgesic (pain-killing) signaling pathway. The ability to interrogate the individual responses of KOR signaling pathways ...


Engineering Dub-Deficient Viral Proteases From Fipv And Pedv Coronaviruses, Daniel T. Wesenberg, Jozlyn R. Clasman, Andrew D. Mesecar 2018 Purdue University

Engineering Dub-Deficient Viral Proteases From Fipv And Pedv Coronaviruses, Daniel T. Wesenberg, Jozlyn R. Clasman, Andrew D. Mesecar

The Summer Undergraduate Research Fellowship (SURF) Symposium

Coronaviruses form a class of viral pathogens lethal to humans and livestock. This issue is compounded by a lack of commercially available treatments or vaccines. In 2014, porcine epidemic diarrhea virus (PEDV) emerged in the United States and accounted for an estimated 7 million porcine deaths. Deaths of humans, companion animals, and livestock caused by coronaviruses highlight the need for therapeutic strategies to combat this devastating disease. One strategy involves engineering papain-like protease 2 (PLP2), an enzyme conserved among coronavirus species that is critical for virus replication and pathogenesis. PLP2’s de-ubiquitinating (DUB) activity aids in the suppression of the ...


Improving Biomanufacturing Production With Novel Elp-Based Transcriptional Regulators, Juya Jeon, Logan R. Readnour, Kevin V. Solomon 2018 Department of Agricultural and Biological Engineering, Purdue University

Improving Biomanufacturing Production With Novel Elp-Based Transcriptional Regulators, Juya Jeon, Logan R. Readnour, Kevin V. Solomon

The Summer Undergraduate Research Fellowship (SURF) Symposium

Microbes can be used to produce valuable drugs, chemicals, and biofuels, but their potential has not been fully realized due to low production yields. To improve biomanufacturing processes and yield, we are developing novel, transcriptional regulators using biosynthesis technology in order to improve cellular health and overall production. Our regulator contains elastin-like polypeptides (ELPs), which make ideal sensors since they exhibit a sharp, inverse phase transition to indicators of cell health such as intracellular pH and ionic strength, and external stimuli such as temperature. We hypothesize that ELP can be fused to transcription factors to control expression of target genes ...


Characterization Of Receptor Expression And Aberrant Gli2 Signaling In Osteosarcoma Cells, Chanelle Hunter, Erik Beadle M.S., Julie Sterling 2018 University of Central Florida

Characterization Of Receptor Expression And Aberrant Gli2 Signaling In Osteosarcoma Cells, Chanelle Hunter, Erik Beadle M.S., Julie Sterling

Chanelle Hunter

Osteosarcoma is the most prevalent primary bone tumor diagnosed in adolescents and young adults and is known for poor prognosis in late stages of detection. Although, there have been many advances in the development of chemotherapy, inhibitors, and combination therapy, there are few existing targeted therapies against the onset of osteosarcoma. Given the complex nature of the bone microenvironment in relation to tumor formation, it is necessary to gain insight into the molecular variations in activation, function, and regulation in order to identify and develop new methods of targeting and drug design. Our objective is to characterize the oncogenic pathway ...


The Rational Design, Synthesis, Characterization, And Biological Evaluation Of Cancer-Targeting Immunostimulatory Peptide-Protein Conjugates And Tripeptides, Keith Smith 2018 Seton Hall University

The Rational Design, Synthesis, Characterization, And Biological Evaluation Of Cancer-Targeting Immunostimulatory Peptide-Protein Conjugates And Tripeptides, Keith Smith

Seton Hall University Dissertations and Theses (ETDs)

With the advent of cancer immunotherapy and the rise in applications of synthetic biologics, there has been a steady decline in the incidence of cancer. Despite this trend, there is an anticipated 1.7 million new cancer cases with an estimated 610,000 deaths expected by the end of 2018.2 Therefore, the call for continued efforts in creating more effective treatment options are still in high demand. In this thesis, the rational design of a semi-synthetic cancer-targeting immunostimulatory peptide-protein bioconjugate—using N-succinimidyl carbamate chemistry is described. This bio-orthogonal chemistry approach was used to conjugate the synthetic Pep42, cancer-targeting peptide ...


Metabolic Plasticity In The Cellular Stress Response, Ying Li 2018 East Tennessee State University

Metabolic Plasticity In The Cellular Stress Response, Ying Li

Electronic Theses and Dissertations

Changes to the metabolism of the cardiomyocyte are driven by complex signaling pathways in order to adjust to stress. For instance, HIF-1α is classically known to upregulate glycolytic metabolism to compensate for oxygen deficiency. Other important effects upon glucose metabolism, which we investigate here more extensively, were also observed. Hearts derived from mice with the cardiac-restricted expression of a stabilized form of HIF-1α are remarkably ischemia stress-tolerant. Here, stable isotope-resolved metabolomic analyses were utilized to investigate glucose cardiometabolism remodeling by HIF-1αduring ischemia. We found that 13C-lactate accumulation was significantly elevated in HIF-1α expressing hearts while paradoxically glycogen was maintained ...


Electron Flow And Management In Living Systems: Advancing Understanding Of Electron Transfer To Nitrogenase, Rhesa N. Ledbetter 2018 Utah State University

Electron Flow And Management In Living Systems: Advancing Understanding Of Electron Transfer To Nitrogenase, Rhesa N. Ledbetter

All Graduate Theses and Dissertations

Nitrogen is a critical nutrient for growth and reproduction in living organisms. Although the Earth’s atmosphere is composed of ~80% nitrogen gas (N2), it is inaccessible to most living organisms in that form. Biological nitrogen fixation, however, can be performed by microbes that harbor the enzyme nitrogenase. This enzyme converts N2 into bioavailable ammonia (NH3) and accounts for at least half of the “fixed”nitrogen on the planet. The other major contributor to ammonia production is the industrial Haber-Bosch process. While the Haber-Bosch process has made significant advances in sustaining the global food supply through the generation of fertilizer ...


Activities And Classroom Demonstrations In Biological Physics: A Resource Document, Philip C. Nelson, William Berner 2018 University of Pennsylvania

Activities And Classroom Demonstrations In Biological Physics: A Resource Document, Philip C. Nelson, William Berner

Department of Physics Papers

We give detailed recipes for a number of classroom demonstrations relevant to biological physics instruction. We developed them mainly for use in 2nd-3rd year undergraduate Physics courses. But you can (and we do) use them for primary school, through high school, up to PhD candidates. You adapt the words for each audience, but a phenomenon is a phenomenon.

We also outline a 1-hour introduction to Python for undergraduate scientific computing.

Finally we document some outcomes from courses taught at U Pennsylvania using these resources and others cited here. This material was presented at a workshop at the AAPT meeting in ...


Production Of A Candidate Recombinant Protein Vaccine For Mannheimia Haemolytica In Lettuce And Tobacco Chloroplasts, Coby K. Martin 2018 The University of Western Ontario

Production Of A Candidate Recombinant Protein Vaccine For Mannheimia Haemolytica In Lettuce And Tobacco Chloroplasts, Coby K. Martin

Electronic Thesis and Dissertation Repository

The cattle industry worldwide is ravaged by bovine respiratory disease (BRD), a bacterial disease caused by Mannheimia haemolytica. Recent efforts to design vaccines against M. haemolytica focus on a virulence factor, leukotoxin, in addition to surface lipoproteins. Plant-based protein production is a safe and inexpensive alternative to traditional methods. Edible vaccines deliver antigens to pharyngeal tissues, which can provide local immunization against M. haemolytica prior to its progression into the lungs. In this project, a chimeric protein containing M. haemolytica antigens was produced in tobacco chloroplasts as a candidate edible vaccine for BRD. Attempts were made to transform lettuce chloroplasts ...


Rgn-259 (Thymosin Β4) Improves Clinically Important Dry Eye Efficacies In Comparison With Prescription Drugs In A Dry Eye Model., Chae Eun Kim, Hynda K Kleinman, Gabriel Sosne, George W Ousler, Kyeongsoon Kim, Sinwook Kang, Jaewook Yang 2018 George Washington University

Rgn-259 (Thymosin Β4) Improves Clinically Important Dry Eye Efficacies In Comparison With Prescription Drugs In A Dry Eye Model., Chae Eun Kim, Hynda K Kleinman, Gabriel Sosne, George W Ousler, Kyeongsoon Kim, Sinwook Kang, Jaewook Yang

Biochemistry and Molecular Medicine Faculty Publications

This study evaluated the clinical activity of RGN-259 (thymosin β4) in comparison with cyclosporine A (CsA), diquafosol (DQS), and lifitegrast (LFA) in a murine model of dry eye. The model was NOD.B10-H2b mice in a 30-40% humidified environment together with daily scopolamine hydrobromide injections for 10 days. After desiccation stress, all drugs were evaluated after 10 treatment days. RGN-259 increased tear production similar to that in the DQS- and LFA-treated mice while CsA was inactive. RGN-259 improved corneal smoothness and decreased fluorescein staining similar to that of LFA group while CsA and DQS were inactive. Corneal epithelial detachment was ...


Cell And Molecular Biology 3e – An Interactive Electronic Textbook, Gerald Bergtrom 2018 University of Wisconsin - Milwaukee

Cell And Molecular Biology 3e – An Interactive Electronic Textbook, Gerald Bergtrom

Cell and Molecular Biology 3e i-Text

Like its predecessor, the interactive electronic text (iText) Cell and Molecular Biology 3e: What We Know & How We Found Out focuses on experimental support for what we know about cell and molecular biology. It was written to serve as the introductory biology course for biology majors with high school chemistry and biology prerequisites as well as for “gateway” cell and molecular biology courses for students with a college-level general biology and general chemistry course background. This is a free Open Education Resource (OER), covered by a Creative Commons CCBY license (for more details, check out the license page of the ...


Preparation And Characterization Of Functionalized Heparin-Loaded Poly-Ɛ-Caprolactone Fibrous Mats To Prevent Infection With Human Papillomaviruses, Daniela Gonzalez, Jorge Ragusa, Peter C. Angeletti, Gustavo F. Larsen 2018 University of Nebraska-Lincoln

Preparation And Characterization Of Functionalized Heparin-Loaded Poly-Ɛ-Caprolactone Fibrous Mats To Prevent Infection With Human Papillomaviruses, Daniela Gonzalez, Jorge Ragusa, Peter C. Angeletti, Gustavo F. Larsen

Chemical and Biomolecular Engineering -- All Faculty Papers

In this study, heparin-loaded poly-ε-caprolactone (PCL) fibrous mats were prepared and characterized based on their physical, cytotoxic, thermal, and biological properties. The main objective of the work described here was to test the hypothesis that incorporation of heparin into a PCL carrier could serve as bio-compatible material capable of inhibiting Human Papillomavirus (HPV) infection. The idea of firmly anchoring heparin to capture soluble virus, vs. a slow heparin release to inhibit a virus in solution was tested. Thus, one material was produced via conventional heparin matrix encapsulation and electrohydrodynamic fiber processing in one step. A second type of material was ...


Effect Of Egta On Sit1 Scramblase Gene Expression And Cell Growth In Tetrahymena Thermophila, Emma Esposito, Theda Knauth, Amelia Ohnstad, Stefanie Otto-Hitt 2018 Carroll College

Effect Of Egta On Sit1 Scramblase Gene Expression And Cell Growth In Tetrahymena Thermophila, Emma Esposito, Theda Knauth, Amelia Ohnstad, Stefanie Otto-Hitt

Life and Environmental Sciences Course-based Research Projects

Scramblase is an enzyme that facilitates the movement of newly synthesized phospholipids from the cytosolic side to the extracellular side of the lipid bilayer. This process is vital for cell membrane repair and growth. In Tetrahymena thermophila, the gene SIT1 encodes for the Scramblase protein, whose functionality is Ca2+-dependent. In this experiment, the concentration of accessible Ca2+ ions was decreased in order to observe whether the change had an aect on the expression of SIT1 and cell growth. It was hypothesized that expression of the SIT1 gene would increase, while cell growth would decrease. To carry out the experiment ...


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