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Functional Characterization Of Recombinant Interleukin (Il)-17a/F1 In The Japanese Pufferfish (Takifugu Rubripes), Jun-ichi Hikima Dr., Koshin Mihara, Shun Maekawa, Han-Ching Wang, Takashi Aoki, Tomoya Kono, Masahiro Sakai 2016 University of Miyazaki, Faculty of Agriculture

Functional Characterization Of Recombinant Interleukin (Il)-17a/F1 In The Japanese Pufferfish (Takifugu Rubripes), Jun-Ichi Hikima Dr., Koshin Mihara, Shun Maekawa, Han-Ching Wang, Takashi Aoki, Tomoya Kono, Masahiro Sakai

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


Interaction Of Spliceosomal U2 Snrnp Protein P14 With Its Branch Site Rna Target, William Perea Vargas 2016 Graduate Center, City University of New York

Interaction Of Spliceosomal U2 Snrnp Protein P14 With Its Branch Site Rna Target, William Perea Vargas

All Dissertations, Theses, and Capstone Projects (2014-Present)

Newly transcribed precursor messenger RNA (pre-mRNA) molecules contain coding sequences (exons) interspersed with non-coding intervening sequences (introns). These introns must be removed in order to generate a continuous coding sequence prior to translation of the message into protein. The mechanism through which these introns are removed is known as pre-mRNA splicing, a two-step reaction catalyzed be a large macromolecular machine, the spliceosome, located in the nucleus of eukaryotic cells. The spliceosome is a protein-directed ribozyme composed of small nuclear RNAs (snRNA) and hundreds of proteins that assemble in a very dynamic process. One of these snRNAs, the U2 snRNA, is ...


Circular Oligodeoxynucleotides (Coligos) As Chemically Synthesized, Promoter-Independent Small Rna Expression Vectors, Lodoe Lama 2016 Graduate Center, City University of New York

Circular Oligodeoxynucleotides (Coligos) As Chemically Synthesized, Promoter-Independent Small Rna Expression Vectors, Lodoe Lama

All Dissertations, Theses, and Capstone Projects (2014-Present)

Small RNAs (sRNA) such as microRNA (miRNA), small interfering RNA (siRNA), and other types of RNA have been found to play many important biological roles in regulation of different cellular process leading to their possible use as therapeutics. However, one of the biggest hurdles in exploiting these sRNAs in therapeutics has been the difficulty in delivering them safely and stably into human tissues. Many delivery approaches have been undertaken to deliver these sRNA, albeit with many shortcomings in terms of cost, stability, and side effects, necessitating the need for alternative approaches. We are exploring an entirely different approach for conveying ...


Force Generation And Contraction Of Random Actomyosin Bundles., Dietmar B. Oelz 2016 Courant Institute (NYU)

Force Generation And Contraction Of Random Actomyosin Bundles., Dietmar B. Oelz

Biology and Medicine Through Mathematics Conference

No abstract provided.


Characterization Of Glycine Rich Proteins From The Salivary Glands Of The Lone Star Tick Amblyomma Americanum, Rebekah Lynn Bullard 2016 University of Southern Mississippi

Characterization Of Glycine Rich Proteins From The Salivary Glands Of The Lone Star Tick Amblyomma Americanum, Rebekah Lynn Bullard

Dissertations

Ticks are blood sucking arthropods that feed on living hosts for up to three weeks. The ticks secrete a multitude of pharmacologically active proteins into the host during feeding which allow the tick to avoid the host immune response, establish a blood pool, and form a firm attachment. The firm attachment is facilitated by the formation of a cement cone which surrounds the tick mouthparts and intertwine between the host skin layers. In this study, gene expression of 44 A. americanum genes was measured throughout the bloodmeal to reveal the differential expression of these genes. Each of the genes tested ...


Investigation Of Novel Functions For Dna Damage Response And Repair Proteins In Escherichia Coli And Humans, Benjamin A. Hilton 2016 East Tennessee State Universtiy

Investigation Of Novel Functions For Dna Damage Response And Repair Proteins In Escherichia Coli And Humans, Benjamin A. Hilton

Electronic Theses and Dissertations

Endogenous and exogenous agents that can damage DNA are a constant threat to genome stability in all living cells. In response, cells have evolved an array of mechanisms to repair DNA damage or to eliminate the cells damaged beyond repair. One of these mechanisms is nucleotide excision repair (NER) which is the major repair pathway responsible for removing a wide variety of bulky DNA lesions. Deficiency, or mutation, in one or several of the NER repair proteins is responsible for many diseases, including cancer. Prokaryotic NER involves only three proteins to recognize and incise a damaged site, while eukaryotic NER ...


Mechanisms Of Regulation Of Tau Ires Mediated Translation, Niza Nemkul 2016 The University of Texas Graduate School of Biomedical Sciences at Houston

Mechanisms Of Regulation Of Tau Ires Mediated Translation, Niza Nemkul

UT GSBS Dissertations and Theses (Open Access)

The translation of most eukaryotic mRNAs occurs in a cap-dependent manner. However, a subset of mRNAs are capable of initiating translation in a cap-independent manner by utilizing sequences in their 5’ UTR called IRES. It was previously shown that the 5’ UTR of the tau mRNA contains an IRES. In this study I show that IRES dependent translation of tau IRES is regulated at multiple levels in order to regulate the expression of the tau protein.

Tau protein is ubiquitously expressed but is concentrated in the brain. In this study, I utilized neural and non-neural cell lines to show that ...


Evaluation Of The Effects Of Radiation On The Positioning Of Histones And Other Proteins, Ashley Elizabeth Foster 2016 University of Nebraska-Lincoln

Evaluation Of The Effects Of Radiation On The Positioning Of Histones And Other Proteins, Ashley Elizabeth Foster

Dissertations and Theses in Biological Sciences

Nucleosomes and the secondary structure of DNA play an important role in many cellular processes that are contingent on DNA accessibility, such as replication, transcriptional regulation, and DNA repair. Recently, there is also growing evidence that the association of histones and DNA to form a nucleosome offers protection from DNA degradation. Specifically, that the inherent structural properties of DNA organization can help protect DNA from exogenous damage such as radiation. In this study, we sought to evaluate the effects of radiation on the positioning of histones and other proteins.

In this experiment, we optimized established protein protocols in Saccharomyces cerevisiae ...


Engineering A Mutation In The Heparin Binding Pocket Of The Human Fibroblast Growth Factor, Roshni Patel 2016 University of Arkansas, Fayetteville

Engineering A Mutation In The Heparin Binding Pocket Of The Human Fibroblast Growth Factor, Roshni Patel

Chemistry & Biochemistry Undergraduate Honors Theses

Fibroblast growth factors (FGFs) are family of proteins that belong to a group of growth factors that are found in mammals and play an important role in angiogenesis, differentiation, organogenesis, and tissue repair. In summary, their main functionality is involved in cell division and proliferation. Because FGFs plays such a vital role in cell proliferation, they are mainly involved in the process of wound healing and injuries. FGF binds to its ligand, heparin—a heavily sulfated glycosaminoglycan. The binding of heparin to FGF occurs through electrostatic interactions, specifically between the negatively charged sulfate groups on heparin and positively charged residues ...


Synthesis Of Microgel Polymers As Catalysts, Hannah N. Miller 2016 University of Arkansas, Fayetteville

Synthesis Of Microgel Polymers As Catalysts, Hannah N. Miller

Chemistry & Biochemistry Undergraduate Honors Theses

New developments in organic synthesis show promise in achieving the best catalytic properties for the hydrolysis of glycosidic bonds through microgel polymers and transition metal complexes. A monomer mix of ethylene glycol dimethacrylate, butyl acrylate, and styrene form miniemulsion polymers after sonication and exposure to UV light. Gravimetrical analysis is used to determine the most suitable polymerization conditions by performing experiments at varying pH values, temperatures, monomer amounts, initiator amounts, and lamp heights. The final data show that the best polymerization conditions are a pH of 10.50 at 0°C with a high monomer ratio, 20% initiator amount, and ...


Deciphering The Role Of Glycine134 In The Human Acidic Growth Factor-1’S Binding To Heparin, Adam W. Burroughs 2016 Department of Chemistry and Biochemistry

Deciphering The Role Of Glycine134 In The Human Acidic Growth Factor-1’S Binding To Heparin, Adam W. Burroughs

Chemistry & Biochemistry Undergraduate Honors Theses

Human acidic fibroblast growth factor 1 (FGF-1) is a potent modulator of cell survival and exhibits a universal role in various physiological processes. Though potent, FGF-1 unbound to heparin is known to show a poor thermal stability and a relatively short in vivo half-life. Much is known about the structure and relation of FGF-1 with heparin yet there is still unknown information regarding the exact role of heparin in stabilizing FGF-1. Thus, the aim of this study is to mutate glycine at position 134 to glutamic acid in wild type FGF1. G134 is located in the heparin binding pocket, thus ...


Investigation Of Parasympathetic Effects Of Lavender Essential Oil In Humans, Haakon Robert Nelson, Rachel Ann Burkhart, Daniel Morin, Nicole Grace Weissenfluh, Michael R. Korn 2016 Liberty University

Investigation Of Parasympathetic Effects Of Lavender Essential Oil In Humans, Haakon Robert Nelson, Rachel Ann Burkhart, Daniel Morin, Nicole Grace Weissenfluh, Michael R. Korn

Montview Liberty University Journal of Undergraduate Research

The purpose of this study will be to investigate the claim that administration of lavender (Lavandula angustifolia) essential oil (topically, orally, and/or respiratorily) produces a relaxative effect in human subjects. This investigation will theoretically be conducted in two stages. Stage one will focus primarily on determining the presence of therapeutic effects and the relative effectiveness of lavender in several application modalities. Stage two will proceed based on findings from stage one. If significant parasympathetic effects are observed in relation to one or more of the lavender oil modalities described above, a more focused investigation will be conducted in stage ...


Subcellular-Level Resolution Maldi-Ms Imaging Of Maize Leaf Metabolites By Maldi-Linear Ion Trap-Orbitrap Mass Spectrometer, Andrew R. Korte, Marna D. Yandeau-Nelson, Basil J. Nikolau, Young-Jin Lee 2016 Iowa State University

Subcellular-Level Resolution Maldi-Ms Imaging Of Maize Leaf Metabolites By Maldi-Linear Ion Trap-Orbitrap Mass Spectrometer, Andrew R. Korte, Marna D. Yandeau-Nelson, Basil J. Nikolau, Young-Jin Lee

Young Jin Lee

A significant limiting factor in achieving high spatial resolution for matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) imaging is the size of the laser spot at the sample surface. Here, we present modifications to the beam-delivery optics of a commercial MALDI-linear ion trap-Orbitrap instrument, incorporating an external Nd:YAG laser, beam-shaping optics, and an aspheric focusing lens, to reduce the minimum laser spot size from ~50 μm for the commercial configuration down to ~9 μm for the modified configuration. This improved system was applied for MALDI-MS imaging of cross sections of juvenile maize leaves at 5-μm spatial resolution using an oversampling ...


Thermal Analysis Of Borosilicate Glass For Its Biological Applications, Gregory Humble 2016 Southern Polytechnic State University

Thermal Analysis Of Borosilicate Glass For Its Biological Applications, Gregory Humble

Symposium of Student Scholars

Borosilicate glass doped with varying wt% of cerium oxide is investigated for biological applications. Thermal studies of each formulation were conducted using an SDT Q600 differential scanning calorimeter. 30mg samples of 350 - 425 μm particle size of each type of glass were heated to 1200°C in order to obtain the glass transition, crystallization, and melting temperatures. Samples were then heated to 900°C at several heating rates, ranging from 2°C/min to 100°C/min, then compared against each other as well as against an undoped borosilicate glass.


Binding Of Oxygen To Human Hemoglobin Within The Erythrocyte Using Icam Spectrophotometry, Kyle K. Hill 2016 University of Nebraska-Lincoln

Binding Of Oxygen To Human Hemoglobin Within The Erythrocyte Using Icam Spectrophotometry, Kyle K. Hill

Student Research Projects, Dissertations, and Theses - Chemistry Department

Many of the spectrophotometric techniques used to determine the properties of intracellular human hemoglobin cannot be utilized due to the turbidity of erythrocyte suspensions. An Integrating Cavity Absorption Meter, or ICAM, allows for absorption measurements of strongly scattering samples in the visible-light region of the spectrum. The spectrum of oxygenated hemoglobin within erythrocytes is significantly different from the absorption spectrum of oxygenated hemoglobin in solution. Studies of the oxygen binding to hemoglobin in erythrocytes allowed the four sequential binding constants (Adair constants) to be determined and compared with those of hemoglobin in solution. The Adair constants for hemoglobin in solution ...


Comparison Of Rna Isolation And Associated Methods For Extracellular Rna Detection By High-Throughput Quantitative Polymerase Chain Reaction, Kahraman Tanriverdi, Alper Kucukural, Ekaterina Mikhalev, Selim E. Tanriverdi, Rosalind Lee, Victor R. Ambros, Jane Freedman 2016 University of Massachusetts Medical School

Comparison Of Rna Isolation And Associated Methods For Extracellular Rna Detection By High-Throughput Quantitative Polymerase Chain Reaction, Kahraman Tanriverdi, Alper Kucukural, Ekaterina Mikhalev, Selim E. Tanriverdi, Rosalind Lee, Victor R. Ambros, Jane Freedman

Victor R. Ambros

MicroRNAs (miRNAs) are small noncoding RNA molecules that function in RNA silencing and posttranscriptional regulation of gene expression. miRNAs in biofluids are being used for clinical diagnosis as well as disease prediction. Efficient and reproducible isolation methods are crucial for extracellular RNA detection. To determine the best methodologies for miRNA detection from plasma, the performance of four RNA extraction kits, including an in-house kit, were determined with miScript miRNA assay technology; all were measured using a high-throughput quantitative polymerase chain reaction (qPCR) platform (BioMark System) with 90 human miRNA assays. In addition, the performances of complementary DNA (cDNA) and preamplification ...


Investigating How Cmvil-10 Isoforms Differ In Cell Signaling And Receptor Engagement, Alec Starzinski 2016 University of San Francisco

Investigating How Cmvil-10 Isoforms Differ In Cell Signaling And Receptor Engagement, Alec Starzinski

Creative Activity and Research Day - CARD

Human Cytomegalovirus (HCMV) is a herpesvirus that infects a majority of the world’s population. There are many viral gene products that aid in virus infection and the establishment of the lifelong latency. UL111A, is a viral gene which, through alternate intron splicing, codes for two protein products cmvIL-10 and LAcmvIL-10 that mimic the structure of human interleukin-10 (hIL-10) to varying degrees. cmvIL-10 has been shown to have a wide range of physiological effects, whereas the effects of LAcmvIL-10 appear to be much more limited in scope. This study seeks to measure the expression levels of LAcmvIL-10 during lytic infection ...


A Netrin-3 Like Protein Is Secreted From Tetrahymena Thermophila, John W. Behnke, David M. Dombrowski, Bryce C. MacTurk, Matthew S. Merical, Kenneth W. Ward, Lauren E. Ward, Daniel J. Stank, Heather G. Kuruvilla 2016 Cedarville University

A Netrin-3 Like Protein Is Secreted From Tetrahymena Thermophila, John W. Behnke, David M. Dombrowski, Bryce C. Macturk, Matthew S. Merical, Kenneth W. Ward, Lauren E. Ward, Daniel J. Stank, Heather G. Kuruvilla

The Research and Scholarship Symposium

Netrin proteins are a family of laminin-related secreted proteins that provide signals for axonal growth and cell migration during vertebrate development. Netrin homologs are expressed throughout the animal kingdom; however, some animals do not express a homolog of any known netrin receptors. We have previously found that the ciliated protozoan, Tetrahymena thermophila, responds to netrin-1 peptide by showing avoidance behavior. In addition, Tetrahymena secrete a protein that is immunologically similar to netrin-1 as detected by ELISA. Since netrin-3, like netrin-1, is a guidance molecule for axons and overlaps signaling pathways with netrin-1 in vertebrates, we hypothesized that netrin-3 may also ...


Further Biochemical Evidence For Secretion Of A Netrin-1 Like Protein From Tetrahymena Thermophila, Matthew S. Merical, Heather G. Kuruvilla 2016 Cedarville University

Further Biochemical Evidence For Secretion Of A Netrin-1 Like Protein From Tetrahymena Thermophila, Matthew S. Merical, Heather G. Kuruvilla

The Research and Scholarship Symposium

Netrin-1 is a pleiotropic signaling molecule first characterized in its role as an axonal guidance molecule. Since then, additional physiological roles for netrin-1 have been found, implicating netrin signaling in processes such as angiogenesis and tumor progression. Netrins are expressed throughout the animal kingdom. We have previously found that Tetrahymena thermophila show avoidance to netrin-1 peptide, and that secreted proteins from Tetrahymena show evidence of netrin-1 activity when assayed by ELISA. In our current study, Western blotting using a polyclonal antibody against netrin-1 showed that a protein of approximately 52 kDa was present in both whole cell extract and secreted ...


Doxycycline Resistance In Plasmodium Falciparum Linked To Single Nucleotide Polymorphisms In The Plasmodium Falciparum Apicoplast Small Subunit Ribosomal Rna (Pfssrrna) Gene, Amanda Wasko 2016 Dominican University of California

Doxycycline Resistance In Plasmodium Falciparum Linked To Single Nucleotide Polymorphisms In The Plasmodium Falciparum Apicoplast Small Subunit Ribosomal Rna (Pfssrrna) Gene, Amanda Wasko

Scholarly and Creative Works Conference

Plasmodium falciparum, a protozoan parasite known as malaria, widely impacts human health; thus antimalarial drug investigations are critical. Doxycycline is a commonly used antimalarial prophylactic, but its mechanism of action is unclear. In prokaryotes, doxycycline works as an antibacterial by disrupting protein translation via the small subunit ribosome. Interestingly, P. falciparum has a small subunit ribosome of prokaryotic origins in the apicoplast, a plastid-like organelle. Therefore, we hypothesized that doxycycline works in P. falciparum by inhibiting protein synthesis via the small subunit ribosomal RNA and that mutations in the gene encoding the P. falciparum apicoplast small subunit ribosomal RNA (pfSSrRNA ...


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