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Effect Of Turmeric On The Promoter Activity Of The Cyp6a8 Gene Of Drosophila Melanogaster, Alexa Stroh 2019 University of Tennessee, Knoxville

Effect Of Turmeric On The Promoter Activity Of The Cyp6a8 Gene Of Drosophila Melanogaster, Alexa Stroh

University of Tennessee Honors Thesis Projects

No abstract provided.


A Rationally Engineered Capsid Variant Of Aav9 For Systemic Cns-Directed And Peripheral Tissue-Detargeted Gene Delivery In Neonates, Dan Wang, Shaoyong Li, Dominic J. Gessler, Jun Xie, Li Zhong, Jia Li, Karen Tran, Kim Van Vliet, Lingzhi Ren, Qin Su, Ran He, Jason E. Goetzmann, Terence R. Flotte, Mavis Agbandje-McKenna, Guangping Gao 2018 University of Massachusetts Medical School

A Rationally Engineered Capsid Variant Of Aav9 For Systemic Cns-Directed And Peripheral Tissue-Detargeted Gene Delivery In Neonates, Dan Wang, Shaoyong Li, Dominic J. Gessler, Jun Xie, Li Zhong, Jia Li, Karen Tran, Kim Van Vliet, Lingzhi Ren, Qin Su, Ran He, Jason E. Goetzmann, Terence R. Flotte, Mavis Agbandje-Mckenna, Guangping Gao

Open Access Articles

Adeno-associated virus (AAV) has provided the gene therapy field with the most powerful in vivo gene delivery vector to realize safe, efficacious, and sustainable therapeutic gene expression. Because many clinically relevant properties of AAV-based vectors are governed by the capsid, much research effort has been devoted to the development of AAV capsids for desired features. Here, we combine AAV capsid discovery from nature and rational engineering to report an AAV9 capsid variant, designated as AAV9.HR, which retains AAV9's capability to traverse the blood-brain barrier and transduce neurons. This variant shows reduced transduction in peripheral tissues when delivered through ...


Mapping Molecular Datasets Back To The Brain Regions They Are Extracted From: Remembering The Native Countries Of Hypothalamic Expatriates And Refugees, Arshad M. Khan, Alice H. Grant, Anais Martinez, Gully APC Burns, Brendan S. Thatcher, Vishwanath T. Anekonda, Benjamin W. Thompson, Zachary S. Roberts, Daniel H. Moralejo, James E. Blevins 2018 University of Texas at El Paso

Mapping Molecular Datasets Back To The Brain Regions They Are Extracted From: Remembering The Native Countries Of Hypothalamic Expatriates And Refugees, Arshad M. Khan, Alice H. Grant, Anais Martinez, Gully Apc Burns, Brendan S. Thatcher, Vishwanath T. Anekonda, Benjamin W. Thompson, Zachary S. Roberts, Daniel H. Moralejo, James E. Blevins

Arshad M. Khan, Ph.D.

This article, which includes novel unpublished data along with commentary and analysis,
focuses on approaches to link transcriptomic, proteomic, and peptidomic datasets mined from
brain tissue to the original locations within the brain that they are derived from using digital atlas
mapping techniques. We use, as an example, the transcriptomic, proteomic and peptidomic
analyses conducted in the mammalian hypothalamus. Following a brief historical overview, we
highlight studies that have mined biochemical and molecular information from the hypothalamus
and then lay out a strategy for how these data can be linked spatially to the mapped locations in a
canonical brain atlas ...


Mathematical Modeling Of Nutrient Signaling And Growth In Saccharomyces Cerevisiae, Amogh P. Jalihal, Pavel Kraikivski, T.M. Murali, John J. Tyson 2018 Virginia Polytechnic Institute and State University

Mathematical Modeling Of Nutrient Signaling And Growth In Saccharomyces Cerevisiae, Amogh P. Jalihal, Pavel Kraikivski, T.M. Murali, John J. Tyson

Biology and Medicine Through Mathematics Conference

No abstract provided.


Inference And Control In Regulatory Genomics, Siddharth Sharma 2018 University of Maryland College Park

Inference And Control In Regulatory Genomics, Siddharth Sharma

Biology and Medicine Through Mathematics Conference

No abstract provided.


Characterizing Virus-Induced Gene Silencing At The Cellular Level With In Situ Multimodal Imaging, Sadie J. Burkhow, Nicole M. Stephens, Yu Mei, Maria Emilia Duenas, Daniel J. Freppon, Geng Ding, Shea C. Smith, Basil J. Nikolau, Young-Jin Lee, Steven A. Whitham, Emily A. Smith 2018 Iowa State University and Ames Laboratory

Characterizing Virus-Induced Gene Silencing At The Cellular Level With In Situ Multimodal Imaging, Sadie J. Burkhow, Nicole M. Stephens, Yu Mei, Maria Emilia Duenas, Daniel J. Freppon, Geng Ding, Shea C. Smith, Basil J. Nikolau, Young-Jin Lee, Steven A. Whitham, Emily A. Smith

Chemistry Publications

Background: Reverse genetic strategies, such as virus-induced gene silencing, are powerful techniques to study gene function. Currently, there are few tools to study the spatial dependence of the consequences of gene silencing at the cellular level.

Results: We report the use of multimodal Raman and mass spectrometry imaging to study the cellular-level biochemical changes that occur from silencing the phytoene desaturase (pds) gene using a Foxtail mosaic virus (FoMV) vector in maize leaves. The multimodal imaging method allows the localized carotenoid distribution to be measured and reveals differences lost in the spatial average when analyzing a carotenoid extraction of the ...


Palmitoylation Of The Sodium Hydrogen Exchanger Isoform 1 (Nhe1): A Novel Post-Translational Protein Modification And Its Regulation Of Nhe1, Emily Pitsch 2018 University of San Diego

Palmitoylation Of The Sodium Hydrogen Exchanger Isoform 1 (Nhe1): A Novel Post-Translational Protein Modification And Its Regulation Of Nhe1, Emily Pitsch

Undergraduate Honors Theses

The sodium-hydrogen exchanger isoform 1 (NHE1) is a ubiquitously expressed transmembrane transporter protein involved in regulating intracellular pH and interacts with a variety of proteins involved in cell signaling, proliferation, and cell motility. The protein has an extended cytosolic tail known as the regulatory region. This project investigates the role of palmitoylation, a reversible fatty acid modification on cysteine amino acids, in the function and behavior of NHE1.


Mechanisms Of Age-Related Prostate Growth And Tumorigenesis, Deon O'Bryant 2018 Atlanta University Center

Mechanisms Of Age-Related Prostate Growth And Tumorigenesis, Deon O'Bryant

Electronic Theses & Dissertations Collection for Atlanta University & Clark Atlanta University

Prostate cancer is the most commonly diagnosed malignancy among men, but few genetic factors that drive prostate cancer initiation have been identified. The WD repeat domain 77 (Wdr77) protein is essential for cellular proliferation when it localizes in the cytoplasm of epithelial cells at the early stage of prostate development. In the adult prostate, it is transported into the nucleus and functions as a co-regulator of the androgen receptor to promote cellular differentiation and prostate function. This developmental process is reversed during prostate tumorigenesis i.e., Wdr77 is translocated from the nucleus into the cytoplasm to drive proliferation of prostate ...


Regulation Of The Pi3-Kinase/Pten Signaling Pathway By Tgf-Β In Prostate Cancer Cells, Mawiyah Kimbrough-Allah 2018 Atlanta University Center

Regulation Of The Pi3-Kinase/Pten Signaling Pathway By Tgf-Β In Prostate Cancer Cells, Mawiyah Kimbrough-Allah

Electronic Theses & Dissertations Collection for Atlanta University & Clark Atlanta University

Transforming growth factor -β (TGF-β) plays an important role in the progression of prostate cancer. It acts as a tumor suppressor in normal epithelial cells but as a tumor promoter in advanced prostate cancer cells. The PI3-kinase pathway has been shown to play integral roles in many cellular processes including cell proliferation, survival, and cell migration in many cell types. PI3-kinase pathway mediates TGF-β effects on prostate cancer cell migration and invasion. Phosphatase and tensin homolog (PTEN), a tumor suppressor gene, inhibits PI3-kinase pathway and is frequently mutated in prostate cancers. In this present study, we investigated possible roles of ...


Cyclic Urethane: A Versatile Handle For Synthetic Peptide Applications, Hader E. Elashal 2018 Seton Hall University

Cyclic Urethane: A Versatile Handle For Synthetic Peptide Applications, Hader E. Elashal

Seton Hall University Dissertations and Theses (ETDs)

While peptides have impeccable potential due to their biological and therapeutic abilities, they are inherently limited in clinical practice due to their instability towards proteolysis and poor bioavailability. In the past few years peptidomimetics have emerged as a powerful class of compounds that overcomes the shortcomings associated with peptides. Peptidomimetics essentially mimic a natural peptide or protein in its chemical composition, structure and its ability to perform a biologically relevant role. In addition to increased stability and bioavailability, peptidomimetics can also have increased receptor binding affinity and selectivity, thereby making these mimics lead compounds in the field of drug design ...


Development Of Lc-Ms For The Identification And Characterization Of Non-Adjacent Dna Photoproduct Formation In G-Quadruplex Forming Sequences, Claudia Posadas 2018 Washington University in St. Louis

Development Of Lc-Ms For The Identification And Characterization Of Non-Adjacent Dna Photoproduct Formation In G-Quadruplex Forming Sequences, Claudia Posadas

Arts & Sciences Electronic Theses and Dissertations

Ultraviolet light is well known to induce cyclobutane pyrimidine dimers (CPD) and pyrimidine (6–4) pyrimidone photoproducts in duplex DNA, which interfere with DNA replication and transcription. Recently, a new class of DNA photoproducts known as anti cyclobutanepyrimidine dimers have been discovered, which form in G-quadruplex forming sequences in solution. G-quadruplex structures have been proposed to form in human DNA telomeres and certain promoters in vivo but evidence for their existence has been lacking. Since anti-cyclobutante pyrimidine dimers have been shown to form in G-quadruplex forming sequences, their formation in irradiated human cells could be used to confirm the ...


Computational Study Of Ligand-Dependent Oligomerization Of Ribonucleotide Reductase, Bill Pham 2018 University of Tennessee, Knoxville

Computational Study Of Ligand-Dependent Oligomerization Of Ribonucleotide Reductase, Bill Pham

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Certain protein oligomerization can be strongly influenced by its ligand-binding status. We constructed a computational method to investigate how ligand-binding and oligomerization can be coupled. We tackle this issue using an approximate approach of studying the properties of individual monomers and how they associate. By connecting the dynamics at monomeric level and the information of oligomer interface, we quantify the synchronization of two types of contact dynamics: (1) between the ligand and its binding pocket, and (2) the contact dynamics at interface. In this work, we applied our methodology on protein ribonucleotide reductase (RNR), which is an essential enzyme for ...


Arabidopsis Thaliana Nip2;1 , A Lactic Acid Transporter, Is Essential For Plant Survival During Hypoxic Stress, Samantha Jean McIntire 2018 University of Tennessee, Knoxville

Arabidopsis Thaliana Nip2;1 , A Lactic Acid Transporter, Is Essential For Plant Survival During Hypoxic Stress, Samantha Jean Mcintire

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

NIPs are nodulin-intrinsic proteins that are specific to plants and involved in transport of water and other uncharged solutes. Arabidopsis thaliana consists of nine NIPgenes.

In the current study, attention has been focused on NIP2;1(NIPsubgroup I), one of the representative Arabidopsis NIPs, in accordance with its cellular and subcellular localization, tissue, transport activity, and biological roles in Arabidopsis.

It has been shown in experiments that AtNIP2;1is especially responsive to hypoxia-induced stress. Hypoxia is a condition in which most of the oxygen has been removed from an environment. In hypoxic environments, plants construct ...


What’S Really Wrong With Medical Research And How To Fix It., Richard Feinman 2018 SUNY Downstate Medical Center

What’S Really Wrong With Medical Research And How To Fix It., Richard Feinman

Journal of Evolution and Health

No abstract provided.


Investigation Into The Amino Acid Characteristic Of Intrinsically Disordered Proteins, Samantha Patterson 2018 University of Wyoming

Investigation Into The Amino Acid Characteristic Of Intrinsically Disordered Proteins, Samantha Patterson

Honors Theses AY 17/18

Caulobacter crescentus, which belongs to the class of Alphaproteobacteria, has a highly organized cytoplasm that includes complex macromolecular structures at the cell poles. These polar complexes are assembled through the activity of a scaffolding protein, known as the Polar Organizing Protein Z (PopZ). PopZ interacts with other proteins via an N-terminal domain that is mostly intrinsically disordered, and contains, an abundance of proline, glutamte, and aspartate (P,E, and D) residues. Surprisingly, scrambling the order of amino acids in the PED region does not affect PopZ function, suggesting that binding affinity and specificity are not dependent on interactions with individual ...


Melatonin-Micronutrients Osteopenia Treatment Study (Mots): A Translational Study Assessing The Effects Of Melatonin, Strontium Citrate, Vitamin D3 And Vitamin K2 On Bone Density, Bone Turnover Markers And Health-Related Quality Of Life In Postmenopausal Osteopenic Women Following A One-Year Double-Blind Randomized Placebo-Controlled Trial And On Osteoblast-Osteoclast Co-Cultures, Sifat Maria 2018 Duquesne University

Melatonin-Micronutrients Osteopenia Treatment Study (Mots): A Translational Study Assessing The Effects Of Melatonin, Strontium Citrate, Vitamin D3 And Vitamin K2 On Bone Density, Bone Turnover Markers And Health-Related Quality Of Life In Postmenopausal Osteopenic Women Following A One-Year Double-Blind Randomized Placebo-Controlled Trial And On Osteoblast-Osteoclast Co-Cultures, Sifat Maria

Electronic Theses and Dissertations

Objective: The purpose of this study was to assess if a novel combination of melatonin and three other natural bone-aiding micronutrients: strontium citrate, vitamins D3 and K2 (MSDK) could improve bone health by modulating the activity of osteoblasts and osteoclasts in favor of balanced bone remodeling and by improving the overall health-related quality of life in postmenopausal osteopenic women.

Methods: The Melatonin-micronutrients Osteopenia Treatment Study (MOTS) is a translational research study that used both clinical and in vitro approaches to assess the efficacy of MSDK on bone health in women and to identify potential mechanisms for its effects ...


Structure Determination Of A Bioengineered Human/Porcine Factor Viii For Hemophilia A Treatment, And Improvements To The Human Factor Viii Model, Ian Smith 2018 Western Washington University

Structure Determination Of A Bioengineered Human/Porcine Factor Viii For Hemophilia A Treatment, And Improvements To The Human Factor Viii Model, Ian Smith

Graduate Student Symposium

Blood coagulation factor VIII (FVIII), is a non-enzymatic cofactor which plays a crucial role in the formation of a stable blood clot. Absence or deficiency of FVIII results in the blood disorder hemophilia A; with symptoms including internal hemorrhaging and the inability to stop bleeding from open wounds. Treatment of hemophilia A relies on infusions of blood, plasma, or protein concentrates to replace FVIII. Unfortunately, approximately 30% of patients receiving replacement FVIII generate pathologic anti-FVIII inhibitory antibodies, which both reduce the effectiveness of the FVIII therapeutic and increase the severity of hemophilia A symptoms. We have determined the molecular structure ...


Synthetic Routes To Bromo-Terminated Phosphonate Films And Alkynyl Pyridine Compounds For Click Coupling, Poornima Sunder 2018 University of Mary Washington

Synthetic Routes To Bromo-Terminated Phosphonate Films And Alkynyl Pyridine Compounds For Click Coupling, Poornima Sunder

Student Research Submissions

Click reactions are a highly versatile class of reactions that produce a diverse range of products. Copper-catalyzed azide-alkyne cycloaddition (CuAAC) click reactions require an azide and a terminal alkyne and produce a coupled product that is “clicked” through a triazole ring that can have a variety of substituents. In this work, bromo-terminated phosphonate films on copper oxide surfaces were explored as the platform for click coupling, as the terminal azide needed for the reaction can be generated through an in situ SN2 reaction with a terminal bromo group. The reactions were characterized using model reactions in solution before being conducted ...


Structure Of Frequency-Interacting Rna Helicase From Neurospora Crassa Reveals High Flexibility In A Domain Critical For Circadian Rhythm And Rna Surveillance, Yalemi Morales, Keith J. Olsen, Jacqueline M. Bulcher, Sean J. Johnson 2018 Utah State University

Structure Of Frequency-Interacting Rna Helicase From Neurospora Crassa Reveals High Flexibility In A Domain Critical For Circadian Rhythm And Rna Surveillance, Yalemi Morales, Keith J. Olsen, Jacqueline M. Bulcher, Sean J. Johnson

Chemistry and Biochemistry Faculty Publications

The FRH (frequency-interacting RNA helicase) protein is the Neurospora crassa homolog of yeast Mtr4, an essential RNA helicase that plays a central role in RNA metabolism as an activator of the nuclear RNA exosome. FRH is also a required component of the circadian clock, mediating protein interactions that result in the rhythmic repression of gene expression. Here we show that FRH unwinds RNA substrates in vitro with a kinetic profile similar to Mtr4, indicating that while FRH has acquired additional functionality, its core helicase function remains intact. In contrast with the earlier FRH structures, a new crystal form of FRH ...


Rapid And Sensitive Detection Of Escherichia Coli O157:H7 Using A Qcm Sensor Based On Aptamers Selected By Whole-Bacterium Selex And A Multivalent Aptamer System, Xiaofan Yu 2018 University of Arkansas, Fayetteville

Rapid And Sensitive Detection Of Escherichia Coli O157:H7 Using A Qcm Sensor Based On Aptamers Selected By Whole-Bacterium Selex And A Multivalent Aptamer System, Xiaofan Yu

Theses and Dissertations

Escherichia coli O157:H7 is one of the top five pathogens contributing to foodborne diseases, causing an estimated 2,138 cases of hospitalization in the US each year. The extremely low infectious dose demands for more rapid and sensitive methods to detect E. coli O157:H7. The objective of this study is to select aptamers specifically binding to E. coli O157:H7 using whole-bacterium SELEX (Systematic Evolution of Ligands by Exponential Enrichment) and to create a multivalent aptamer system by rolling circle amplification (RCA) with the selected aptamer sequence for sensitive detection of E. coli O157:H7 using a quartz ...


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