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5,253 full-text articles. Page 1 of 158.

Functional Characterization Of Recombinant Interleukin (Il)-17a/F1 In The Japanese Pufferfish (Takifugu Rubripes), Jun-ichi Hikima Dr., Koshin Mihara, Shun Maekawa, Han-Ching Wang, Takashi Aoki, Tomoya Kono, Masahiro Sakai 2016 University of Miyazaki, Faculty of Agriculture

Functional Characterization Of Recombinant Interleukin (Il)-17a/F1 In The Japanese Pufferfish (Takifugu Rubripes), Jun-Ichi Hikima Dr., Koshin Mihara, Shun Maekawa, Han-Ching Wang, Takashi Aoki, Tomoya Kono, Masahiro Sakai

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


Identification And Characterization Of Heat Shock Transcription Factor 1 Isoforms In Orange-Spotted Grouper (Epinephelus Coioides), Tingyu Wang 2016 National Cheng Kung University

Identification And Characterization Of Heat Shock Transcription Factor 1 Isoforms In Orange-Spotted Grouper (Epinephelus Coioides), Tingyu Wang

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


Rna-Seq Analysis In Gills Of Atlantic Salmon (Salmo Salar) Infected With Newly Identified Isav Strains In The Atlantic Canada Region, Francis LeBlanc 2016 Fisheries and Oceans Canada

Rna-Seq Analysis In Gills Of Atlantic Salmon (Salmo Salar) Infected With Newly Identified Isav Strains In The Atlantic Canada Region, Francis Leblanc

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


The Expression Analysis Of Per1 Gene In Fugu, Takifugu Rubripes Administered With Immunostimulants, Goshi Nishi, Jun-ichi Hikima Dr., Masahiro Sakai, Tomoya Kono 2016 University of Miyazaki, Faculty of Agriculture

The Expression Analysis Of Per1 Gene In Fugu, Takifugu Rubripes Administered With Immunostimulants, Goshi Nishi, Jun-Ichi Hikima Dr., Masahiro Sakai, Tomoya Kono

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


Implication Of Biological And Synthetic Nanoparticles In Evolution And Development Of Antibiotic Resistance Relevant For Aquaculture, Petter Langlete 2016 University of Oslo

Implication Of Biological And Synthetic Nanoparticles In Evolution And Development Of Antibiotic Resistance Relevant For Aquaculture, Petter Langlete

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


Identification Of Qtls For Resistance Against Rhizoctonia Solani And Phoma Glycinicola In Soybeans (Glycine Max L. Merr), Herbert Sserunkuma 2016 University of Nebraska-Lincoln

Identification Of Qtls For Resistance Against Rhizoctonia Solani And Phoma Glycinicola In Soybeans (Glycine Max L. Merr), Herbert Sserunkuma

Theses, Dissertations, and Student Research in Agronomy and Horticulture

A QTL (Quantitative trait locus) is a chromosome location of a gene controlling a specific phenotypic trait. The trait maybe governed by multiple genes. Fungal pathogens are responsible for over 50% of all soybean diseases. Rhizoctonia solani Kühn causes seedling dumping off, root and hypocotyl rots and other disease in soybeans. Phoma glycinicola de Gruyter & Boerema causes Red leaf blotch disease, predominantly in Sub-Saharan Africa. There is no reported complete resistance against these fungal pathogens in soybeans. Reaction to R.solani is reportedly a quantitative trait controlled by major and minor genes. Three QTLs contributing to reaction to R. solani ...


Hiv Vaccines: Progress, Limitations And A Crispr/Cas9 Vaccine, Omar A. Garcia Martinez 2016 Augustana College, Rock Island Illinois

Hiv Vaccines: Progress, Limitations And A Crispr/Cas9 Vaccine, Omar A. Garcia Martinez

Biology: Student Scholarship & Creative Works

ABSTRACT: The HIV-1 pandemic continues to thrive due to ineffective HIV-1 vaccines. Historically, the world’s most infectious diseases, such as polio and smallpox, have been eradicated or have come close to eradication due to the advent of effective vaccines. Highly active antiretroviral therapy is able to delay the onset of AIDS but can neither rid the body of HIV-1 proviral DNA nor prevent further transmission. A prophylactic vaccine that prevents the various mechanisms HIV-1 has to evade and attack our immune system is needed to end the HIV-1 pandemic. Recent advances in engineered nuclease systems, like the CRISPR/Cas9 ...


Phylogenetic Analysis Of Human Cytomegalovirus Pus27 And Pus28: Ascertaining An Independent Or Linked Evolutionary History, Jessica A. Scarborough 2016 University of San Francisco

Phylogenetic Analysis Of Human Cytomegalovirus Pus27 And Pus28: Ascertaining An Independent Or Linked Evolutionary History, Jessica A. Scarborough

Undergraduate Honors Theses

Human cytomegalovirus (HCMV) is a widespread pathogen that is particularly skilled at evading immune detection and defense mechanisms, largely due to extensive co-evolution with its host’s immune system. One aspect of this co-evolution involves the acquisition of four virally encoded GPCR chemokine receptor homologs, products of the US27, US28, UL33 and UL78 genes. G protein-coupled receptors (GPCR) are the largest family of cell surface proteins, found in organisms from yeast to humans. In this research, phylogenetic analysis was used to investigate the origins of the US27 and US28 genes, which are adjacent in the viral genome. The results indicate ...


Life In The Underworld: Anchialine Cave Biology In The Era Of Speleogenomics, Jorge L. Pérez-Moreno, Thomas M. Iliffe, Heather D. Bracken-Grissom 2016 Florida International University

Life In The Underworld: Anchialine Cave Biology In The Era Of Speleogenomics, Jorge L. Pérez-Moreno, Thomas M. Iliffe, Heather D. Bracken-Grissom

International Journal of Speleology

Anchialine caves contain haline bodies of water with underground connections to the ocean and limited exposure to open air. Despite being found on islands and peninsular coastlines around the world, the isolation of anchialine systems has facilitated the evolution of high levels of endemism among their inhabitants. The unique characteristics of anchialine caves and of their predominantly crustacean biodiversity nominate them as particularly interesting study subjects for evolutionary biology. However, there is presently a distinct scarcity of modern molecular methods being employed in the study of anchialine cave ecosystems. The use of current and emerging molecular techniques, e.g., next-generation ...


Beyond Structural Genomics: Computational Approaches For The Identification Of Ligand Binding Sites In Protein Structures, Dario Ghersi, Roberto Sanchez 2016 University of Nebraska at Omaha

Beyond Structural Genomics: Computational Approaches For The Identification Of Ligand Binding Sites In Protein Structures, Dario Ghersi, Roberto Sanchez

Dario Ghersi

t Structural genomics projects have revealed structures for a large number of proteins of unknown function. Understanding the interactions between these proteins and their ligands would provide an initial step in their functional characterization. Binding site identification methods are a fast and cost-effective way to facilitate the characterization of functionally important protein regions. In this review we describe our recently developed methods for binding site identification in the context of existing methods. The advantage of energy-based approaches is emphasized, since they provide flexibility in the identifi- cation and characterization of different types of binding sites


Molblocks: Decomposing Small Molecule Sets And Uncovering Enriched Fragments, Dario Ghersi, Mona Singh 2016 University of Nebraska at Omaha

Molblocks: Decomposing Small Molecule Sets And Uncovering Enriched Fragments, Dario Ghersi, Mona Singh

Dario Ghersi

The chemical structures of biomolecules, whether naturally occurring or synthetic, are composed of functionally important building blocks. Given a set of small molecules—for example, those known to bind a particular protein—computationally decomposing them into chemically meaningful fragments can help elucidate their functional properties, and may be useful for designing novel compounds with similar properties. Here we introduce molBLOCKS, a suite of programs for breaking down sets of small molecules into fragments according to a predefined set of chemical rules, clustering the resulting fragments, and uncovering statistically enriched fragments. Among other applications, our software should be a great aid ...


Systematic Assessment Of Accuracy Of Comparative Model Of Proteins Belonging To Different Structural Fold Classes, Subrata Chakrabarty, Dario Ghersi, Roberto Sanchez 2016 South Dakota State University

Systematic Assessment Of Accuracy Of Comparative Model Of Proteins Belonging To Different Structural Fold Classes, Subrata Chakrabarty, Dario Ghersi, Roberto Sanchez

Dario Ghersi

In the absence of experimental structures, comparative modeling continues to be the chosen method for retrieving structural information on target proteins. However, models lack the accuracy of experimental structures. Alignment error and structural divergence (between target and template) influence model accuracy the most. Here, we examine the potential additional impact of backbone geometry, as our previous studies have suggested that the structural class (all-α, αβ, all-β) of a protein may influence the accuracy of its model. In the twilight zone (sequence identity ≤ 30%) and at a similar level of target-template divergence, the accuracy of protein models does indeed follow the ...


Computer Simulations Of Heterologous Immunity: Highlights Of An Interdisciplinary Cooperation, Claudia Calcagno, Roberto Puzone, Yanthe E. Pearson, Yiming Cheng, Dario Ghersi, Liisa K. Selin, Raymond M. Welsh, Franco Celada 2016 New York University School of Medicine

Computer Simulations Of Heterologous Immunity: Highlights Of An Interdisciplinary Cooperation, Claudia Calcagno, Roberto Puzone, Yanthe E. Pearson, Yiming Cheng, Dario Ghersi, Liisa K. Selin, Raymond M. Welsh, Franco Celada

Dario Ghersi

The relationship between biological research and mathematical modeling is complex, critical, and vital. In this review, we summarize the results of the collaboration between two laboratories, exploring the interaction between mathematical modeling and wet-lab immunology. During this collaboration several aspects of the immune defence against viral infections were investigated, focusing primarily on the subject of heterologous immunity. In this manuscript, we emphasize the topics where computational simulations were applied in conjunction with experiments, such as immune attrition, the growing and shrinking of cross-reactive T cell repertoires following repeated infections, the short and long-term effects of cross-reactive immunological memory, and the ...


Automated Identification Of Binding Sites Forphosphorylated Ligands In Protein Structures, Dario Ghersi, Roberto Sanchez 2016 University of Nebraska at Omaha

Automated Identification Of Binding Sites Forphosphorylated Ligands In Protein Structures, Dario Ghersi, Roberto Sanchez

Dario Ghersi

Phosphorylation is a crucial step in many cellular processes, ranging from metabolic reactions involved in energy transformation to signaling cascades. In many instances, protein domains specifically recognize the phosphogroup. Knowledge of the binding site provides insights into the interaction, and it can also be exploited for therapeutic purposes. Previous studies have shown that proteins interacting with phosphogroups are highly heterogeneous, and no single property can be used to reliably identify the binding site. Here we present an energy-based computational procedure that exploits the protein three-dimensional structure to identify binding sites involved in the recognition of phosphogroups. The procedure is validated ...


Genome-Wide Compensatory Changes Accompany Drug-Selected Mutations In The Plasmodium Falciparum Crt Gene, Hongying Jiang, Jigar J. Patel, Ming Yi, Jianbing Mu, Jinhui Ding, Robert Stephens, Roland A. Cooper, Michael T. Ferdig, Xin-zhuan Su 2016 Old Dominion University

Genome-Wide Compensatory Changes Accompany Drug-Selected Mutations In The Plasmodium Falciparum Crt Gene, Hongying Jiang, Jigar J. Patel, Ming Yi, Jianbing Mu, Jinhui Ding, Robert Stephens, Roland A. Cooper, Michael T. Ferdig, Xin-Zhuan Su

Roland A. Cooper

Mutations in PfCRT (Plasmodium falciparum chloroquine-resistant transporter), particularly the substitution at amino acid position 76, confer chloroquine (CQ) resistance in P. falciparum. Point mutations in the homolog of the mammalian multidrug resistance gene (pfmdr1) can also modulate the levels of CQ response. Moreover, parasites with the same pfcrt and pfmdr1 alleles exhibit a wide range of drug sensitivity, suggesting that additional genes contribute to levels of CQ resistance (CQR). Reemergence of CQ sensitive parasites after cessation of CQ use indicates that changes in PfCRT are deleterious to the parasite. Some CQR parasites, however, persist in the field and grow well ...


Exploring Barriers To The Adoption Of Pharmacogenomic Technology In The Clinical Setting By Clinical Healthcare Providers, Jason Sudia 2016 Seton Hall University

Exploring Barriers To The Adoption Of Pharmacogenomic Technology In The Clinical Setting By Clinical Healthcare Providers, Jason Sudia

Seton Hall University Dissertations and Theses (ETDs)

The changing landscape of healthcare in the US has created new questions about how to best provide cost-effective, individualized care. Personalized medicine and more specifically, pharmacogenomic technology have offered new tools for healthcare providers to use to increase the efficacy, safety, and cost-effectiveness of care. However, these tools are not being utilized to their predicted extent in the clinical setting. This study utilized Rogers’ Diffusion of Innovations theory to investigate some of the reasons why. A multi-question survey, the PI-created, Pharmacogenomic Adoption Instrument (PAI) ©, was developed to assess the knowledge, attitudes and experience concerning pharmacogenetic technology in a spectrum of ...


Identification Of Candidate Genes Underlying An Iron Efficiency Quantitative Trait Locus In Soybean, Gregory A. Peiffer, Keith E. King, Andrew J. Severin, Gregory D. May, Silvia R. Cianzio, Shun Fu Lin, Nicholas C. Lauter, Randy C. Shoemaker 2016 Iowa State University

Identification Of Candidate Genes Underlying An Iron Efficiency Quantitative Trait Locus In Soybean, Gregory A. Peiffer, Keith E. King, Andrew J. Severin, Gregory D. May, Silvia R. Cianzio, Shun Fu Lin, Nicholas C. Lauter, Randy C. Shoemaker

Andrew Severin

Prevalent on calcareous soils in the United States and abroad, iron deficiency is among the most common and severe nutritional stresses in plants. In soybean (Glycine max) commercial plantings, the identification and use of iron-efficient genotypes has proven to be the best form of managing this soil-related plant stress. Previous studies conducted in soybean identified a significant iron efficiency quantitative trait locus (QTL) explaining more than 70% of the phenotypic variation for the trait. In this research, we identified candidate genes underlying this QTL through molecular breeding, mapping, and transcriptome sequencing. Introgression mapping was performed using two related near-isogenic lines ...


Genome-Wide Transposon Mutagenesis Reveals A Role For Po157 Genes In Biofilm Development In Escherichia Coli O157:H7 Edl933, Supraja Puttamreddy, Nancy A. Cornick, F. Chris Minion 2016 Iowa State University

Genome-Wide Transposon Mutagenesis Reveals A Role For Po157 Genes In Biofilm Development In Escherichia Coli O157:H7 Edl933, Supraja Puttamreddy, Nancy A. Cornick, F. Chris Minion

Nancy Cornick

Enterohemorrhagic Escherichia coli O157:H7, a world-wide human food-borne pathogen, causes mild to severe diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome. The ability of this pathogen to persist in the environment contributes to its dissemination to a wide range of foods and food processing surfaces. Biofilms are thought to be involved in persistence, but the process of biofilm formation is complex and poorly understood in E. coli O157:H7. To better understand the genetics of this process, a mini-Tn5 transposon insertion library was constructed in strain EDL933 and screened for biofilm-negative mutants using a microtiter plate assay. Ninety-five of 11 ...


A Comparative Study Of Isolation In Headwater Fishes, Bjorn Victor Schmidt 2016 University of Southern Mississippi

A Comparative Study Of Isolation In Headwater Fishes, Bjorn Victor Schmidt

Dissertations

Headwater resident fishes may be prone to a high rate of population fragmentation within river networks because large streams have habitat conditions outside of their preferred ecological niche and may limit gene flow in the dendritic ecological network. To investigate patterns of population structure, asymmetrical gene flow, and influences on genetic distance and isolation from connecting habitat pathways, species specific ecological traits, and basin scale characteristics, a multi-species, multi-regional study was performed. Six headwater species of fish from four taxonomic groupings were sampled for genetic material in three regions of paired neighbor drainages and then genotyped for eight microsatellite loci ...


Engineering A Mutation In The Heparin Binding Pocket Of The Human Fibroblast Growth Factor, Roshni Patel 2016 University of Arkansas, Fayetteville

Engineering A Mutation In The Heparin Binding Pocket Of The Human Fibroblast Growth Factor, Roshni Patel

Chemistry & Biochemistry Undergraduate Honors Theses

Fibroblast growth factors (FGFs) are family of proteins that belong to a group of growth factors that are found in mammals and play an important role in angiogenesis, differentiation, organogenesis, and tissue repair. In summary, their main functionality is involved in cell division and proliferation. Because FGFs plays such a vital role in cell proliferation, they are mainly involved in the process of wound healing and injuries. FGF binds to its ligand, heparin—a heavily sulfated glycosaminoglycan. The binding of heparin to FGF occurs through electrostatic interactions, specifically between the negatively charged sulfate groups on heparin and positively charged residues ...


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