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Mitochondrial Aspects Of Neuronal Pathology In Triple-Transgenic Alzheimer’S Disease Mice, John Zachary Cavendish 2021 West Virginia University

Mitochondrial Aspects Of Neuronal Pathology In Triple-Transgenic Alzheimer’S Disease Mice, John Zachary Cavendish

Graduate Theses, Dissertations, and Problem Reports

Alzheimer’s disease (AD) is a fatal, progressive neurodegenerative disease afflicting millions of people in the United States alone and is the only one of the top leading causes of morbidity and mortality with no effective disease-modifying therapies. It is the most common form of dementia, affecting one in three people over the age of 85. While the hallmarks of the disease include accumulation of beta-amyloid-based extracellular plaques and hyperphosphorylated tau-based intracellular neurofibrillary tangles, treatment strategies centered on removing or mitigating these components of AD have all failed in humans. Mitochondrial dysfunction has been increasingly recognized as an early and ...


Specific Degradation Of Misfolded Mutant P53 In Cancer Cells Through The Use Of Natural Compounds Targeting Mutant P53 And Dnaja1 Proteins, Mohamed A.A. Alalem 2020 Children's Mercy Hospital

Specific Degradation Of Misfolded Mutant P53 In Cancer Cells Through The Use Of Natural Compounds Targeting Mutant P53 And Dnaja1 Proteins, Mohamed A.A. Alalem

Research Days

No abstract provided.


Multi-Generational Effects Of ∆9-Tetrahydrocannabinol Exposure On Gene Expression In Liver Tissue, Kayla Lovitt 2020 University of Mississippi

Multi-Generational Effects Of ∆9-Tetrahydrocannabinol Exposure On Gene Expression In Liver Tissue, Kayla Lovitt

Honors Theses

Cannabis is the most commonly used, cultivated, and trafficked illicit drug worldwide. Increased availability and acceptance of cannabis and cannabinoid-containing products provide the necessity for understanding how these substances influence aging. In this study, zebrafish (Danio rerio) were exposed to concentrations of Δ9-tetrahydrocannabinol (THC) (0.08, 0.4, 2 µM) during embryonic-larval development, the effects on aging were measured 30 months later and in the offspring of the exposed fish (F1 generation. We observed results indicating a biphasic and hormetic effect. Treatment with the lowest concentration of THC significantly increased egg production, while higher concentrations resulted in impaired ...


The Current Neuroscientific Understanding Of Alzheimer's Disease, Rachel A. Brandes 2020 University of Tennessee, Knoxville

The Current Neuroscientific Understanding Of Alzheimer's Disease, Rachel A. Brandes

Pursuit - The Journal of Undergraduate Research at the University of Tennessee

Alzheimer’s disease is a degenerative neurological illness characterized by the deterioration of brain regions implicated in memory and cognitive function. While researchers have yet to find a cure or effective treatment, they have gained a better understanding of its pathology and development. Through years of neuroscience research, scientists have discovered much of what happens in the brain during Alzheimer’s disease onset and how this causes its symptoms; many hypotheses regarding this aspect of the illness involve temporal lobe atrophy, neurofibrillary tangles, and amyloid plaques. Although Alzheimer’s disease affects millions of people every day, it seems that most ...


Alternative Polyadenylation Modulates Expression Of Pro-Fibrotic Proteins And Contributes To Lung Fibrosis, Junsuk Ko 2020 The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences

Alternative Polyadenylation Modulates Expression Of Pro-Fibrotic Proteins And Contributes To Lung Fibrosis, Junsuk Ko

The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences Dissertations and Theses (Open Access)

Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease which affects about 5 to 8 million individuals in the world. Despite the high prevalence, there is currently no cure for IPF, and the cause of this disease is still unclear. Our laboratory and collaborators have shown that nudix hydrolase 21 (NUDT21, which is also known as cleavage factor 25, CFIm25) is a key regulator of alternative polyadenylation (APA). NUDT21 depletion causes 3’UTR shortening via APA leading to enhanced mRNA stability and protein translation. This NUDT21 reduction promotes tumor growth in glioblastoma by enhancing expression of oncogenes. Cancer and IPF ...


158— Optimization And Development Of The Luria-Delbrück Experiment For Geneseo Genetics Labs, Abigail Scheffler 2020 SUNY Geneseo

158— Optimization And Development Of The Luria-Delbrück Experiment For Geneseo Genetics Labs, Abigail Scheffler

GREAT Day

The classic genetics experiment by Salvador Luria and Max Delbruck showed that mutations are present prior to selection rather than arising in response to selective pressures. In this experiment, we used Escherichia coli as our model organism and the antibiotics streptomycin and rifampicin to show students in a laboratory setting how this hypothesis can be applied. Specifically, we optimized antibiotic concentrations and the amount of bacterial cells to use in order for the activity to run smoothly and consistently in a student laboratory. We found that the ideal conditions are 1000 cells/ml and 25 micrograms/ml of streptomycin. These ...


Characterization Of Nasal Isolates Of Staphylococcus Aureus From Concordia's Campus, Jeenue Vang 2020 Concordia University St. Paul

Characterization Of Nasal Isolates Of Staphylococcus Aureus From Concordia's Campus, Jeenue Vang

Research and Scholarship Symposium Posters

Research students from the Concordia University, St. Paul Science Department has collected over 1,000 nasal swabs over the past 5 years in an attempt to characterize nasal isolates of Staphylococcus aureus from healthy individuals. S. aureus is a common commensal bacterium found on the skin, in the nares, or in the vaginal tract of approximately 30% of the population. While S. aureus is typically harmless when it is growing in those areas, it can also pose a public health risk as it can act as an opportunistic pathogen to cause a variety of infections, such as toxic shock syndrome ...


Data On Enrichment Of Chitosan Nanoparticles For Intranasal Delivery Of Oligonucleotides To The Brain, Vasyl Sava, Oksana Fihurka, Anastasia Khvorova, Juan Sanchez-Ramos 2020 University of South Florida

Data On Enrichment Of Chitosan Nanoparticles For Intranasal Delivery Of Oligonucleotides To The Brain, Vasyl Sava, Oksana Fihurka, Anastasia Khvorova, Juan Sanchez-Ramos

Open Access Articles

Data on preparation and characterization of chitosan-based nanoparticles (NP) carrying small interfering RNA (siRNA) for non-invasive gene therapy is presented. Polyelectrolyte complexation method was carried out in diluted concentrations to obtain relatively small (less than 200 nm) NP. To provide substantial dose of siRNA within tolerable volume of intranasal administration the NP were subjected to enrichment process. Offered here NP fabrication does two steps process comprise provisional and enriched preparations? The differences between these preparations were analyzed with hydrodynamic size distribution and zeta potential measurements. The effect of siRNA lipophilicity on NP physical instability was also tested. Biological evaluation of ...


A Review Of The Scientific Rigor, Reproducibility, And Transparency Studies Conducted By The Abrf Research Groups., Sheenah M Mische, Nancy C Fisher, Susan M Meyn, Katia Sol Church, Rebecca L Hegstad-Davies, Frances Weis-Garcia, Marie Adams, John M Ashton, Kym M Delventhal, Julie A Dragon, Laura Holmes, Pratik Jagtap, Christopher E Mason, Magnus Palmblad, Brian C Searle, Christoph W Turck, Kevin L Knudtson 2020 Institute for Systems Biology, Seattle, Washington, USA.

A Review Of The Scientific Rigor, Reproducibility, And Transparency Studies Conducted By The Abrf Research Groups., Sheenah M Mische, Nancy C Fisher, Susan M Meyn, Katia Sol Church, Rebecca L Hegstad-Davies, Frances Weis-Garcia, Marie Adams, John M Ashton, Kym M Delventhal, Julie A Dragon, Laura Holmes, Pratik Jagtap, Christopher E Mason, Magnus Palmblad, Brian C Searle, Christoph W Turck, Kevin L Knudtson

Articles, Abstracts, and Reports

Shared research resource facilities, also known as core laboratories (Cores), are responsible for generating a significant and growing portion of the research data in academic biomedical research institutions. Cores represent a central repository for institutional knowledge management, with deep expertise in the strengths and limitations of technology and its applications. They inherently support transparency and scientific reproducibility by protecting against cognitive bias in research design and data analysis, and thedy have institutional responsibility for the conduct of research (research ethics, regulatory compliance, and financial accountability) performed in their Cores. The Association of Biomolecular Resource Facilities (ABRF) is a FASEB-member scientific ...


Polyphenol-Rich Purple Corn Pericarp Extract Adversely Impacts Herbivore Growth And Development, Mandeep Tayal, Pavel Somavat, Isabella Rodriguez, Tina Thomas, Bradely Christoffersen, Rupesh R. Kariyat 2020 The University of Texas Rio Grande Valley

Polyphenol-Rich Purple Corn Pericarp Extract Adversely Impacts Herbivore Growth And Development, Mandeep Tayal, Pavel Somavat, Isabella Rodriguez, Tina Thomas, Bradely Christoffersen, Rupesh R. Kariyat

Biology Faculty Publications and Presentations

Plant secondary metabolites such as terpenes, phenolics, glycosides, and alkaloids play various functional roles including pigmentation, foliar and floral volatile synthesis, hormonal regulation, and direct and indirect defenses. Among these, phenolic compounds are commonly found in plants, but vary in the distribution of their specific compounds among plant families. Polyphenols, including anthocyanins and tannins, are widely distributed and have been well documented for their roles- primarily in plant pigmentation and also in plant defenses. However, commercialization of such compounds for use in insect pest management is severely hampered by expensive, inefficient, and time-consuming extraction protocols. Using a recently developed inexpensive ...


Determination Of Iron-Reducing Bacterial Activities In Lake Sediments., Alexandra Kahn 2020 The University of Akron

Determination Of Iron-Reducing Bacterial Activities In Lake Sediments., Alexandra Kahn

Williams Honors College, Honors Research Projects

Algal blooms are growing rampantly in lacustrine systems due to an increase of phosphorus, a nutrient for algae. Phosphorus is being released into the environment causing overpopulation and eutrophication which damages the ecosystem. The availability of phosphorus is affected by the solubility of iron which is regulated through iron reducing and iron oxidizing bacteria. Phosphorus adheres to insoluble Fe(III), which prohibits algae from utilizing it, while phosphorus does not attach to soluble Fe(II) and therefore it remains available to algae. The purpose of this study was to determine how bacteria influence iron solubility and what are the ideal ...


The Cardiovascular Response To Acute Hypoxic Conditions In Danio Rerio, Bryce Fetterman 2020 The University of Akron

The Cardiovascular Response To Acute Hypoxic Conditions In Danio Rerio, Bryce Fetterman

Williams Honors College, Honors Research Projects

There are many fish that can withstand hypoxia levels. One in particular is the zebrafish (Danio rerio). The zebrafish is a small-sized, Cyprinid teleost fish, and has been used in a laboratory setting for many reasons. A few reasons being that they are widely available, easy to care for, and the cost for these fish are very low. There are various reasons why these fish are used as a model. One main reason is because their cardiovascular system is one of the first developing organs. This allows for better resolution when studying the cardiovascular system. The purpose of this study ...


Non-Invasive Method For Leptin Supplementation In Zebrafish (Danio Rerio), Regan McNamara 2020 The University of Akron

Non-Invasive Method For Leptin Supplementation In Zebrafish (Danio Rerio), Regan Mcnamara

Williams Honors College, Honors Research Projects

I tested the hypothesis that recombinant leptin protein can be introduced to zebrafish in vivo through non-invasive soaking in a solution containing the protein. One way to study various molecules’ effects in vivo is through intraperitoneal or intracerebroventricular injections during the embryonic or larval stage, which is invasive, difficult to administer, and can have a high mortality rate. 48 hours post fertilization (hpf) zebrafish were soaked in a His-tagged recombinant leptin protein solution at 10 nM and 100 nM concentrations (produced by Genscript). After soaking, zebrafish larvae were washed extensively to remove all recombinant protein on their exterior before homogenization ...


Tick-Borne Infections In New Hampshire: An Evaluation Of The Diagnostic Process In A Local Patient Population, Katherine Anderson 2020 University of New Hampshire, Durham

Tick-Borne Infections In New Hampshire: An Evaluation Of The Diagnostic Process In A Local Patient Population, Katherine Anderson

Honors Theses and Capstones

Overall, approximately 95 percent of reported cases of vector-borne disease were associated with ticks, making these the most medically important group of arthropods in the United States.1 Despite the prevalence of tick-borne infections, the process for the diagnosis of this condition is not well studied. This study aims to analyze data from a pool of 100 patients who underwent testing for tick-borne disease in the same institution in Dover, New Hampshire during the most recent peak tick season of 2019. Information utilized in this study included: patient age, sex, location of testing (inpatient versus outpatient), diagnostic testing methods used ...


Influence Of Salinity On Copper-Ligand Formation Constants, Fatimah Barnawi 2020 Wilfrid Laurier University

Influence Of Salinity On Copper-Ligand Formation Constants, Fatimah Barnawi

Theses and Dissertations (Comprehensive)

Natural Organic Matter (NOM) is known to reduce metal, such as copper, toxicity in aquatic environments. Copper is essential for organisms, but elevated concentrations of dissolved copper can potentially be toxic. The toxicity of copper is related to its bioavailability, which is influenced by toxicity modifying factors, such as NOM (quantified as Dissolved Organic Carbon (DOC) in mg C/L), alkalinity, pH and major cation and anion concentrations. The principles are the same for fresh and saltwater, but the influence of high salt concentrations, such as in estuaries, and associated activity corrections, can modify NOM complexation of metals (i.e ...


The Exploration Of Nanotoxicological Copper And Interspecific Saccharomyces Hybrids, Matthew Joseph Winans PhD 2020 West Virginia University

The Exploration Of Nanotoxicological Copper And Interspecific Saccharomyces Hybrids, Matthew Joseph Winans Phd

Graduate Theses, Dissertations, and Problem Reports

Nanotechnology takes advantage of cellular biology’s natural nanoscale operations by interacting with biomolecules differently than soluble or bulk materials, often altering normal cellular processes such as metabolism or growth. To gain a better understanding of how copper nanoparticles hybridized on cellulose fibers called carboxymethyl cellulose (CMC) affected growth of Saccharomyces cerevisiae, the mechanisms of toxicity were explored. Multiple methodologies covering genetics, proteomics, metallomics, and metabolomics were used during this investigation. The work that lead to this dissertation discovered that these cellulosic copper nanoparticles had a unique toxicity compared to copper. Further investigation suggested a possible ionic or molecular mimicry ...


The Impact Of Aging And Mechanical Injury On Alveolar Epithelial And Macrophage Responses In Acute Lung Injury And Inflammation, Michael S. Valentine 2020 Virginia Commonwealth University

The Impact Of Aging And Mechanical Injury On Alveolar Epithelial And Macrophage Responses In Acute Lung Injury And Inflammation, Michael S. Valentine

Theses and Dissertations

Patients with severe lung pathologies, such as Acute Respiratory Distress Syndrome (ARDS), often require mechanical ventilation as a clinical intervention; however, this procedure frequently exacerbates the original pulmonary issue and produces an exaggerated inflammatory response that potentially leads to sepsis, multisystem organ failure, and mortality. This acute lung injury (ALI) condition has been termed Ventilator-Induced Lung Injury (VILI). Alveolar overdistension, cyclic atelectasis, and biotrauma are the primary injury mechanisms in VILI that lead to the loss of alveolar barrier integrity and pulmonary inflammation. Stress and strains during mechanical ventilation are believed to initiate alveolar epithelial mechanotransduction signaling mechanisms that contribute ...


The Potential For Dickeya Dianthicola To Be Vectored By Two Common Insect Pests Of Potatoes, Jonas K. Insinga 2019 University of Maine

The Potential For Dickeya Dianthicola To Be Vectored By Two Common Insect Pests Of Potatoes, Jonas K. Insinga

Electronic Theses and Dissertations

Dickeya dianthicola (Samson) causing blackleg and soft rot was first detected in potatoes grown in Maine in 2014. Previous work has suggested that insects, particularly aphids, may be able to vector bacteria in this genus between plants, but no conclusive work has been done to confirm this theory. In order to determine whether insect-mediated transmission is likely to occur in potato fields, two model potato pests common in Maine were used: the Colorado potato beetle (Leptinotarsa decimlineata Say) and the green peach aphids (Myzus persicae Sulzer). Olfactometry and recruitment experiments evaluated if either insect discriminates between infected and uninfected foliage ...


Green Roof System Integrated Soil Methods, Jude R. Vallon, Ivan L. Guzman 2019 CUNY New York City College of Technology

Green Roof System Integrated Soil Methods, Jude R. Vallon, Ivan L. Guzman

Publications and Research

Large metropolitan areas like NYC are seeking to integrate sustainability into retrofitting buildings for the development of green infrastructure. Among the many environmental issues of urbanization, the UHI (urban heat island) effect and storm water runoff are of particular interest when it comes building structures. Individual buildings can contribute towards mitigating these effects with implementation of vegetative rooftops, i.e. Green Roofs. Commercial buildings are currently receiving government incentives and new constructions are required to include green roof installations. However, if existing buildings are considering the addition of a green roof on an existing roof, they have to factor in ...


Mechanisms And Consequences Of Myb Gene Activation In Salivary Gland Tumors, Candace Frerich 2019 University of New Mexico

Mechanisms And Consequences Of Myb Gene Activation In Salivary Gland Tumors, Candace Frerich

Biomedical Sciences ETDs

Salivary gland adenoid cystic carcinoma (ACC) is an aggressive tumor with a tendency to infiltrate surrounding nerves and metastasize to distant sites. The standard treatment often fails to control local tumor recurrence and distant metastases and no approved targeted therapeutic options exist for these tumors. The goal of our studies was to reveal the molecular mechanisms driving ACC tumor development and novel drug targets to improve patient morbidity and mortality.

We first analyzed clinical and RNA-sequencing (RNA-seq) data for 68 formalin-fixed paraffin-embedded (FFPE) ACC tumor samples and described previously unappreciated molecular heterogeneity that predicts patient outcome. The poor outcome subgroup ...


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