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Search For Palladin, An Actin-Associated Protein, In Pig Retinal Pigmented Epithelium And Its Role In Epithelial-Mesenchymal Transition, Katrina Powell 2021 Bellarmine University

Search For Palladin, An Actin-Associated Protein, In Pig Retinal Pigmented Epithelium And Its Role In Epithelial-Mesenchymal Transition, Katrina Powell

Undergraduate Theses

This study investigates the expression of Palladin, a phosphoprotein product of the PALLD gene, in the retinal pigmented epithelium (RPE). Palladin is an actin cross-linking protein and plays a role in cell adhesion and motility. Published reports have demonstrated that a down regulation of Palladin in colon cancer cells results in a reorganization of the actin cytoskeleton, causing the cells to lose their typical shape, become proliferative and migratory. This process is otherwise known as epithelial-mesenchymal transition (EMT). A similar EMT phenomenon is observed when the RPE is exposed to the vitreous humor in patients with proliferative vitreoretinopathy (PVR). In ...


Stage Of Estrous Cycle Influences Behavior After Social Stress, Alexander Jonah Rodriguez 2021 University of South Dakota

Stage Of Estrous Cycle Influences Behavior After Social Stress, Alexander Jonah Rodriguez

Honors Thesis

Since women are almost twice as likely to develop major depression compared to men, research into this and other potentially debilitating mood disorders using behavioral studies should involve females despite hormonal, physiological, and potential behavior changes due to reproductive cyclicity. Until recently, most studies exclusively used males, thus limiting results of previous research to half of the population. Using adult female C57BL/6N mice as model organisms, we exposed them to novel forms of social stress across five days which encompassed the four-day estrous cycle to determine if the stages of the estrous cycle had an impact on behavior when ...


Comparing Cognition In Women Between The Ages Of (18-40) And (40-70), Annalyn Smith 2021 University of Arkansas, Fayetteville

Comparing Cognition In Women Between The Ages Of (18-40) And (40-70), Annalyn Smith

Biological Sciences Undergraduate Honors Theses

Currently, 5.8 million Americans are living with Alzheimer’s disease (AD), which is a degenerative brain disease. It is currently the sixth-leading cause of death in the United States (Alzheimer’s Association, 2019). AD also possesses a financial burned on the United States, estimated to cost $290 billion in 2019. Sufferers of AD insidiously lose their memory, personality, and judgment abilities until there are no longer recognizable by their loved ones. Currently, the best chance suffers of AD have is to catch the disease early on to slow its effects. This is due to the fact that treatment can ...


Intermachine Reliability Of The Velotron During Wingate Anaerobic Testing, Caleb Christie 2021 Utah State University

Intermachine Reliability Of The Velotron During Wingate Anaerobic Testing, Caleb Christie

All Graduate Plan B and other Reports

Purpose: The purpose of this study was to evaluate inter-machine reliability of the Velotron electromagnetically-braked cycle ergometer for the Wingate anaerobic test (WAnT) with power-trained athletes. Methods: Anaerobic athletes participating in football or track and field at a National Collegiate Athletics Association Division I program were recruited for participation in the study. Each participant visited the laboratory 3 times (1 familiarization trial, 2 experimental trials) within 2 weeks and at least 24 hours between visits. Participants completed a “practice trial” on the first visit to familiarize themselves with the 30-second WAnT. The WAnT procedure included a 5-minute warmup at a ...


Perivascular Adipose Tissue In Relation To Diet, Thermogenesis And Cardiovascular Health, Ginger Paquette, Caitlin Stieber, Ashely Soucy, Benjamin Tero, Lucy Liaw 2021 University of Southern Maine

Perivascular Adipose Tissue In Relation To Diet, Thermogenesis And Cardiovascular Health, Ginger Paquette, Caitlin Stieber, Ashely Soucy, Benjamin Tero, Lucy Liaw

Thinking Matters Symposium

Adipose tissue is a diverse and crucial component to vascular health due to its role in energy storage and heat production. The primary function of white adipose tissue (WAT) is energy storage while the function of mitochondria-rich brown adipose tissue (BAT) is heat production. Perivascular adipose tissue (PVAT), which surrounds blood vessels, contains both WAT and BAT adipocytes. Dietary calorie restriction is associated with increased lifespan with decreased adiposity. Increased prevalence of WAT-like PVAT, due to high-fat diets and obesity, leads to increased metabolic disfunction and cardiovascular-disease. We used a calorie-restriction model in C57BL6/J mice to test the hypothesis ...


Effect Of Temperature On Questing Activity Of Ixodes Scapularis In A Laboratory Setting, Molly Meagher 2021 University of Southern Maine

Effect Of Temperature On Questing Activity Of Ixodes Scapularis In A Laboratory Setting, Molly Meagher

Thinking Matters Symposium

Understanding deer tick (Ixodes scapularis) behavior is increasingly important due to the species’ northerly range expansion, and consequently, increased human contact. Deer ticks are a major public health concern, as they are the primary vector of Lyme disease and other serious diseases. Differences in questing behavior are of considerable interest as fluctuating weather due to climate change effect the species’ range expansion and time spent questing. Although previous research has examined temperature preferences of tick behavior, there are conflicting reports about direct effects on questing activity. Therefore, I addressed the relationship between temperature and questing behavior of adult deer ticks ...


Validation Of A Novel Trpm8 Knockout Mouse Model, Bilan Mohamed 2021 University of Southern Maine

Validation Of A Novel Trpm8 Knockout Mouse Model, Bilan Mohamed

Thinking Matters Symposium

Recent studies suggest that the use of thermoregulatory treatments that affect brown fat may help curb obesity. However it is unknown how these treatments may affect bone homeostasis. Our work has focused on the transient receptor potential melastatin (TRPM8) protein, which is responsible for detecting colder temperatures in sensory neurons. Previous work within the Motyl laboratory has found that Trpm8 plays a role in bone acquisition. Mice with a global deletion of the Trpm8 gene have reduced trabecular bone volume fraction due to reduced bone formation by osteoblasts. However, it is unclear whether sensory neuron or osteoblast-mediated expression of Trpm8 ...


Importance Of Understanding Genetic Predisposition, Andrew Carter 2021 Southern Maine Community College

Importance Of Understanding Genetic Predisposition, Andrew Carter

Thinking Matters Symposium

Hypersensitivity reactions can be sudden and are often fatal. Many of these reactions occur as a result of allergies to prescribed medication. Adverse drug reactions or (ADR’s) were show in a recent study to affect over 6% of hospitalized patients and resulted in over 100,000 deaths a year in the US alone. This poster will review the importance of understanding genetic predisposition, through articles about possible genetic causes of allergies to penicillin and other beta-lactams. Changes in a specific HLA gene located on chromosome 6 showed a correlation to penicillin reactions in over 600,000 participants. Another study ...


Interrogating The Functions Of The Histone H2a Repression Domain In Relation To Cell-Cycle Progression In Saccharomyces Cerevisiae, Taylor Nattress 2021 Susquehanna University

Interrogating The Functions Of The Histone H2a Repression Domain In Relation To Cell-Cycle Progression In Saccharomyces Cerevisiae, Taylor Nattress

Senior Scholars Day

In eukaryotic cells, DNA is wrapped around histone proteins to form the basic repeating unit of chromatin, the nucleosome core particle. Most nucleosomes consist of two copies each of histones: H2A and H2B in a heterodimer and histones H3 and H4 in a heterotetramer. Because of their intimate association with DNA, histones regulate all DNA-templated processes such as transcription, DNA replication, and DNA damage response and repair. Several studies have illustrated the importance of a small portion of the histone H2A amino terminal domain which regulates global transcription. This domain is responsible for the repression of ~4% of the yeast ...


S.O.S. For Covid-19 (Subjective And Objective Screening): Are Asymptomatic Cases Truly Without Warning Signs?, Nathan Hughes 2021 Wayne State Unviersity

S.O.S. For Covid-19 (Subjective And Objective Screening): Are Asymptomatic Cases Truly Without Warning Signs?, Nathan Hughes

Honors College Theses

This study investigates the usefulness of five objective signs, evaluates relationships between objective signs with symptoms of COVID-19, and assesses the accuracy of ambient forehead temperature with tympanic temperature. Data were collected at Wayne State University. Blood oxygen levels and blood pressure were measured via pulse oximeters and blood pressure cuff. Body temperature was measured with a thermometer via the temple and the forehead. Smell was tested with two scented markers. None of the 29 participants confirmed a positive COVID-19 test. Therefore, only relationships between vital sign measurements were reported. No significant correlations were revealed upon analysis. Infrared and temporal ...


The Effect Of A Single Dose Of Beetroot Juice On Speed, Strength, And Power In Healthy Recreationally Active Females., Maya A. Hammer, Ryan Kenney, Katie Price, Chandler Riley, Alyssa Riley, Courtney Merrill, Adam Pennell, Rachel Tan 2021 Pepperdine University

The Effect Of A Single Dose Of Beetroot Juice On Speed, Strength, And Power In Healthy Recreationally Active Females., Maya A. Hammer, Ryan Kenney, Katie Price, Chandler Riley, Alyssa Riley, Courtney Merrill, Adam Pennell, Rachel Tan

Seaver College Research And Scholarly Achievement Symposium

Nitrate-rich beetroot juice (BR) improves muscle contraction which is relevant for intense intermittent-type sports. However, few studies have examined the effect of BR ingestion on performance using sport-specific exercise protocols. Moreover, there is a scarcity of research that include female participants which limits real world application given that females potentially have different responses to BR ingestion. The purpose of the present study was to examine the effect of BR supplementation on speed, acceleration, strength and power before and after fatigue in females. In a double-blind, randomized crossover design, 15 recreationally active females consumed BR and nitrate-depleted placebo juice (PL) ~2 ...


The Effects Of Nitrate-Rich Beetroot Juice Supplementation On Nonverbal Executive Function In Healthy Recreationally Active Females, Chandler F. Riley, Alyssa Riley, Maya A. Hammer, Ryan Kenney, Katie Price, Courtney Merrill, Adam Pennell, Rachel Tan 2021 Pepperdine University

The Effects Of Nitrate-Rich Beetroot Juice Supplementation On Nonverbal Executive Function In Healthy Recreationally Active Females, Chandler F. Riley, Alyssa Riley, Maya A. Hammer, Ryan Kenney, Katie Price, Courtney Merrill, Adam Pennell, Rachel Tan

Seaver College Research And Scholarly Achievement Symposium

Nitrate-rich beetroot juice (BR) supplementation has been reported to preserve executive function (i.e. decision making and reaction time) before and after a simulated soccer match in recreationally active males, which may be due to enhanced cerebral blood flow. However, the literature examining the physiological response following BR ingestion in females is scarce, which hampers the extrapolation of results since physiological sex-differences may exist. Therefore, the purpose of this study was to assess if BR ingestion influenced executive function in an unfatigued and fatigued state in healthy recreationally active females. In a double-blind, randomized crossover design, 20 females consumed 140 ...


The Effect Of Changing Substrate On Arctic Aquatic Invertebrates Abundance, Tom Dolman 2021 University of Maine at Farmington

The Effect Of Changing Substrate On Arctic Aquatic Invertebrates Abundance, Tom Dolman

Michael D. Wilson Symposium

Climate change is directly affecting tundra ecosystems in northern regions, and warming temperatures have caused discontinuous permafrost and thawing sediments across the region. This project investigates how increasing erosion and the foraging patterns of migratory snow geese may degrade habitat for aquatic invertebrates in the upper Mast River, located in Wapusk National Park, Manitoba, Canada. In the past two decades, many of the important species of aquatic invertebrates have shown declines. Declining invertebrate populations are predicted to affect aquatic ecosystems and decrease the resources available to shorebirds and waterfowl, which breed and migrate through this area.


Toxic Effect Of Crotalus Adamanteus Acidic Phospholipase A2 On Mcf-7 Cell Line, Daniel J. Petra 2021 University of South Dakota

Toxic Effect Of Crotalus Adamanteus Acidic Phospholipase A2 On Mcf-7 Cell Line, Daniel J. Petra

Honors Thesis

We are investigating the effect of Crotalus adamanteus acidic phospholipase A2 on MCF-7 cells using the MTS assay. Understanding these interactions and isolated effects is critical to developing new ways to treat envenomation. By understanding the effects of individual toxins within a whole venom, we are set to better understand the effects of the whole venom and investigate synergistic actions between venom toxins. In this paper, we are quantifying the amount of MCF-7 cell death caused by Crotalus adamanteus phospholipase A2 on MCF-7 using the MTS assay. Analysis of the amount of cells death caused by the phospholipase ...


Laboratory Techniques In Public Health: Bi-520 Introduction To Public Health Lab Manual, Anuradha Srivastava 2021 CUNY Queensborough Community College

Laboratory Techniques In Public Health: Bi-520 Introduction To Public Health Lab Manual, Anuradha Srivastava

Open Educational Resources

This laboratory manual for a foundations course Public Health is unique in the sense that very rarely, a foundations course in the field has a lab component offered with the lecture. This lab manual provides basic laboratory exercises that could be useful to introduce undergraduate students to the basics of public health laboratory practices.


Morph- And Sex-Specific Differences In Corticosterone Of The Arizona Tiger Salamander (Ambystoma Mavortium Nebulosum), Megan Zerger 2021 Murray State University

Morph- And Sex-Specific Differences In Corticosterone Of The Arizona Tiger Salamander (Ambystoma Mavortium Nebulosum), Megan Zerger

Scholars Week

Life history morph, sex, and body condition are traits that may influence stress within salamander populations because of differences in physiology and environmental conditions. Given widespread declines and the effects chronic stress can have on amphibian health, it is important to understand within-population drivers of stress and how population level variation may influence population viability. Thus, the objective of our study was to assess how corticosterone varies within the Arizona tiger salamander (Ambystoma mavortium nebulosum) population at the Mexican Cut Nature Preserve. We used a non-invasive skin swabbing method to collect baseline and elevated corticosterone from paedomorph (aquatic morph; N ...


Novel Uplc-Ms/Ms Method For Standardization Of Niazimicin Content In Edible Seeds And Leaves Of Moringa Oleifera Lam., Eman M. Abdelsayed, Amira Abdel Motaal, Rasha S. Hanafi 2021 University of Hertfordshire hosted by Global Academic Foundation, Egypt

Novel Uplc-Ms/Ms Method For Standardization Of Niazimicin Content In Edible Seeds And Leaves Of Moringa Oleifera Lam., Eman M. Abdelsayed, Amira Abdel Motaal, Rasha S. Hanafi

Journal of Food and Drug Analysis

Moringa oleifera (MO) is a highly nutritious plant, whose leaves and seed pods are consumed in Africa, Asia, sub-Himalayan regions and South America. A novel ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the standardization of the bioactive thiocarbamate compound from MO, niazimicin (NZ) in seeds and leaves, is developed, optimized and validated according to the International Conference on Harmonization (ICH) guidelines, using desipramine as the internal standard. Multiple reaction monitoring detection of transitions 358.05>106.86 and 266.38 > 193.04 with collision energy of 25V and 40V, respectively were used. A gradient was optimized ...


Functional Influence Of 14-3-3 (Ywha) Proteins In Mammals, Elizabeth Barley, Santanu De 2021 Nova Southeastern University

Functional Influence Of 14-3-3 (Ywha) Proteins In Mammals, Elizabeth Barley, Santanu De

Mako: NSU Undergraduate Student Journal

The 14-3-3 (YWHA) proteins are homologous, ubiquitous, and conserved in most organisms ranging from plants to animals and play important roles in regulating key cellular events such as cell signaling, development, apoptosis, etc. These proteins consist of seven isoforms in mammals, termed under Greek alphabetization: beta (β), gamma (γ), epsilon (ε), eta (η), tau/theta (τ), sigma (σ), and zeta (ζ). Each of these isoforms can interact with a plethora of binding partners and has been shown to serve a distinct role in molecular crosstalk, biological processes, and disease susceptibility. Protein 14-3-3 isoforms are scaffolding proteins capable of forming homodimers ...


Influence Of 2,5-Dimethoxy-4-Iodoamphetamine (Doi) On Ethanol Preference And Consumption In C57bl/6 Male Mice, Alaina M. Jaster, Sam Gottlieb, Michael Miles, Javier González-Maeso 2021 Virginia Commonwealth University

Influence Of 2,5-Dimethoxy-4-Iodoamphetamine (Doi) On Ethanol Preference And Consumption In C57bl/6 Male Mice, Alaina M. Jaster, Sam Gottlieb, Michael Miles, Javier González-Maeso

Graduate Research Posters

Substance use disorders (SUD) account for a large number of mental health diagnosis in the United States and around the world. Approximately 13.6 million adults 26 or older and 5.1 million young adults (ages 18-24) battled with a SUD in 2017. Alcohol Use Disorder (AUD) alone effects nearly 6% of the adult population within the United States. This creates a substantial burden on the individual, with alcohol being the third-leading cause of preventable death in the United States. Few treatments for AUD exist, with no new FDA-approved therapeutic treatments within the last 15 years. Additionally, the limited treatments ...


Mitochondrial Aspects Of Neuronal Pathology In Triple-Transgenic Alzheimer’S Disease Mice, John Zachary Cavendish 2021 West Virginia University

Mitochondrial Aspects Of Neuronal Pathology In Triple-Transgenic Alzheimer’S Disease Mice, John Zachary Cavendish

Graduate Theses, Dissertations, and Problem Reports

Alzheimer’s disease (AD) is a fatal, progressive neurodegenerative disease afflicting millions of people in the United States alone and is the only one of the top leading causes of morbidity and mortality with no effective disease-modifying therapies. It is the most common form of dementia, affecting one in three people over the age of 85. While the hallmarks of the disease include accumulation of beta-amyloid-based extracellular plaques and hyperphosphorylated tau-based intracellular neurofibrillary tangles, treatment strategies centered on removing or mitigating these components of AD have all failed in humans. Mitochondrial dysfunction has been increasingly recognized as an early and ...


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