Entomology Commons^™

Rna Interference As A Tool For The Functional Analysis Of Genes In The Colorado Potato Beetle, Leptinotarsa Decemlineata (Say), Ashley Danielle Yates

Dissertations and Student Research in Entomology

RNA interference (RNAi) is a naturally occurring phenomenon in eukaryotes in which a double-stranded RNA (dsRNA) suppresses the expression of a target gene. RNAi has markedly changed the way in which functional genetics studies are performed, especially in non-model organisms. In insects, the efficacy of RNAi is influenced by several factors, including the species and the methods of dsRNAs delivery.

The Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say), is a pest of the plant family Solanaceae. RNAi in the CPB is of interest for potential use in insect management and as a tool to study the interaction with host plants ...

Conclusive Evidence Of Replication Of A Plant Virus In Honeybees Is Lacking, W. Allen Miller, Jimena Carrillo-Tripp, Bryony C. Bonning, Adam G. Dolezal, Amy L. Toth

Entomology Publications and Papers

The recent article by Li et al. (1) lacks adequate evidence to support the authors’ assertion that a plant virus propagates or replicates in honeybees. Instead, it is possible that tobacco ringspot virus (TRSV) virions associate with the honeybee and parasitic Varroa mites in the absence of TRSV replication.

Investigations Into Sensory Ecology And Gene Evolution Of The Pea Aphid (Acyrthosiphon Pisum), Swapna R. Purandare

Dissertations and Theses in Biological Sciences

Organisms use environmental cues to gather information required to perform activities that are essential for their survival and reproduction, such as searching for food, avoiding danger, and finding mates. They respond to the acquired information by changing their behavior or physiology, which may result in increased fitness. Due to the fundamental importance of information in an organism’s life, it is important to understand its acquisition, processing, and the organism’s response to it. In the work presented here, we used the pea aphid (Acyrthosiphon pisum), an insect that produces multiple phenotypes, or morphs, that are genetically identical, but differ ...

Morphometric Studies On Subadult Liza Subviridis And Sillago Sihama From Sonmiani Bay (Miani Hor), Balochistan, Pakistan, Muhammad A. Gondal, Naureen A. Qureshi, Noor U. Saher

Journal of Bioresource Management

Length-weight relationship of Liza subviridis and Sillago sihama (Family: Mugilidae; Class: Pisces and Family: Sillaginidae; Class: Pisces) in subadults sampled from Somiani Bay Balochistan, Pakistan during 2002, 2003 and 2006 was analyzed. Liza subviridis showed positive allometry (3.23) in 2002, and negative allometry during 2003 (2.95) and 2006 (1.95). Sub adults of Sillago sihama showed positive allometry during 2002 (3.10) and 2003 (3.13). An isometric condition (3.02) was observed in the samples of Sillago sihama collected from Bhaira in 2006.

On Individual, Sex And Age Differentiation Of Indian House Crow (Corvus Splendens) Call: A Preliminary Study In Potohar, Pakistan, Waqas Hameed, Inayat Ullah, Safia Janjua, Fakhar -I- Abbas, Afsar Mian

Journal of Bioresource Management

Considering importance of acoustics studies in population biology, 500 calls of the Indian House Crow (Corvus splendens) were recorded in morning - mid-afternoon hours (January-February, 2009) from 23 sites of urban areas of Potahar (Punjab, Pakistan). Calls were recorded using Sony CFS 1030 S sound records (sampling rate = 48 KHz) and edited using Sound Analysis Pro (Version 1.02). software using FFT method rate 50%, data window 9.27 ms, advanced window 1.36 ms. Through editing of calls, we selected 60 (37 ♂♂, 17 ♀♀, 6 Juvenile ♂♂) good quality spectrograms for detailed analysis. Spectrograms were characterized by rapid frequency modulations using 6 ...

Review: Pollination, Pollinated And Pollinators Interaction In Pakistan, Mohammad Irshad, Elizabeth Stephen

Journal of Bioresource Management

The agriculture sector is important in the overall economy and export earnings of Pakistan. Pollination is an essential ecosystem service that depends on symbiosis between species, the pollinated and the pollinator. Animal mediated pollination contributes to the sexual production of over 90% species of modern angiosperms. Effective pollination results in increased crop production, quality improvement and more seed production. Many fruits, vegetables, edible oil crops, stimulant crops and nuts are highly dependent on bee pollination. Worldwide value of pollinators is €153 billion (217 billion US dollars). The production value of pollinated dependent crop in Pakistan is quantified to be 1 ...

Research Note: Contribution To Mushroom Flora Of Rawalpindi-Islamabad, Pakistan, Kishwar Sultana, Naveeda Riaz, Gulshan Irshad, Ali Nauman Khan

Journal of Bioresource Management

Seven hymenomycetous fungi (mushrooms) were collected from the twin cities area during July 2008. These were identified as the members of the order Agaricales, class Basidiomycetes. They belong to five genera: *Clitocybe fragrans, *Collybia cookie, *Coprinus radians, *Coprinus sterculinus, *Volvariella bingensis, Volvariella parvula, and Termitomyces microcarpus. Five of them, marked with asterisk were reported for the first time from Pakistan.

Diagnostic Accuracy Of C-Reactive Protein In Neonatal Sepsis, Sidra Younis, Muhammad Ali Sheikh, Amjad Ali Raza

Journal of Bioresource Management

Sepsis is the most common cause of neonatal mortality and is responsible for 30-50% of total neonatal deaths each year in developing countries. The objective of the study was to determine the diagnostic accuracy of C-reactive protein (CRP) in neonatal sepsis. Fifty nine consecutive patients with risk factors and clinical features suggestive of CRP sepsis were selected as per operational definition and fulfilling the inclusion and exclusion criteria. Detailed physical examination was carried out. Blood sample for culture and CRP was taken from all the patients. Results of blood culture and CRP were noted down in the performa. Statistical analysis ...

Mechanisms Of Differential Toxicity Between Honey Bee (Apis Mellifera L.) Castes With An Emphasis On Coumaphos, Lizette Dahlgren

Dissertations and Student Research in Entomology

Acaricides are used to treat honey bee (Apis mellifera L.) colonies to control the parasitic Varroa mite (Varroa destructor Anderson & Trueman), a worldwide threat to honey bee health. As such, these compounds act as drugs to mitigate bee losses but may also stress the bees. This dissertation quantifies differences between queen and worker tolerance of five acaricides and clarifies the honey bee mechanism of tolerance for coumaphos. Selected acaricides were topically applied to adult queen and worker bees to generate dose-response curves and LD₅₀s. Twenty-four hours after treatment, queens were 3-times more tolerant of tau-fluvalinate and 6-times more ...

Diversity And Ecology Of Host-Seeking Mosquitoes In Irrigated Agro-Ecosystems Of Clay County, Nebraska, Alister K. Bryson

Dissertations and Student Research in Entomology

In the United States, Nebraska has the third highest incidence of human West Nile virus (WNV). Since WNV was first detected in the state in 2002, 3,422 confirmed cases and 57 deaths have been reported. Irrigated agro-ecosystems, which have been associated with elevated WNV incidences in other states, are prevalent in Nebraska. The objectives of this investigation were to 1) characterize mosquito abundance and diversity in irrigated agro-ecosystems, and 2) evaluate associations of two primary vectors of WNV, Culex tarsalis and Culex pipiens, with irrigation methods (sprinkler vs. surface) and crop type (corn vs. soybean). Investigations were conducted ...

Development Modeling Of Lucilia Sericata And Phormia Regina (Diptera: Calliphoridae), Amanda L. Roe

Dissertations & Theses in Natural Resources

The relationship between insect development and temperature has been well established and has a wide range of uses, including using blow flies for postmortem (PMI) interval estimations in death investigations. To use insects in estimating PMI, we must be able to determine the insect age at the time of discovery and backtrack to time of oviposition. Unfortunately, existing development models of forensically important insects are only linear approximations and do not take into account the curvilinear properties experienced at extreme temperatures. A series of experiments were conducted with two species of forensically important blow flies (Lucilia sericata and Phormia regina ...

Pelargonium (Geraniaceae) And The Long-Tongued Fly Pollination Guild: Coevolution And Polymorphism In A Specialized Pollination System, Emily M. Blank

Honors Scholar Theses

In South Africa, a group of long-tongued flies and the flowering plants they pollinate form a unique and diverse pollination guild system almost entirely endemic to the Greater Cape Floristic Region. This system makes up only a small portion of the biodiversity of the Region, but it is likely a key to understanding the evolutionary processes that have lead to the observed biodiversity. Aside from its unusual pollinators, this guild system is of particular interest due to the extremely labile morphology of both the fly species and the associated flowering plant species, which geographically vary significantly in proboscis lengths and ...

Testing For Positive Edge Responses In A Fragmented Landscape In The Eastern Tiger (Papilio Glaucus) And The Spicebush (P. Troilus) Swallowtail Butterflies, Jenna C. Siu

University of Western Ontario - Electronic Thesis and Dissertation Repository

Landscape changes such as habitat fragmentation and habitat loss are contributing to a global decline in biodiversity. While habitat fragmentation research has mainly focused on species that avoid edges, or the boundaries between different landcover types (negative edge response), a hypothesized resource distribution model predicts that species that require complementary resources in different landcovers will be most abundant at edges (positive edge response). Adults of Eastern Tiger (Papilio glaucus) and Spicebush (P. troilus) swallowtail butterflies require forests for oviposition sites and meadows for nectar resources. I examined the relative abundance and flight orientation of both species in relation to the ...

Overview Of The Integrated Pest Management (Ipm) Terrain And Activities In Furtherance Of The Walmart Initiative To Purchase Fresh Vegetables From Local Growers In Alabama, Franklin Quarcoo, Conrad Bonsi

Professional Agricultural Workers Journal

Tuskegee University has been providing various types of technical expertise to limited resource farmers who have been supplying Walmart with collard greens, watermelons and purple hull peas. A number of pests bedevil the cultivation of these crops; cost-effective management methods for these pests are needed. The objectives of this paper are to document the IPM activities associated with supplying produce to Walmart; summarize pest problems encountered on the afore-stated crops; recommend IPM methods suitable for limited resource farmers; and suggest other activities that ensure that farmers incur even less pest-related crop losses. Anthracnose was the major pest encountered on watermelons ...

A Review Of New World Laemophloeus Dejean (Coleoptera: Laemophloeidae): 2. Neotropical Species With Antennal Club Of Three Antennomeres, Michael C. Thomas

Insecta Mundi

Eleven Neotropical species of Laemophloeus Dejean with antennal clubs composed of three antennomeres are reviewed, diagnosed, and illustrated. Six of the species are described as new: L. capitesculptus Thomas, n. sp., L. corporeflavus Thomas, n. sp., L. dozieri Thomas, n. sp., L. insulatestudinorum Thomas, n. sp., L. planaclavatus Thomas, n. sp., and L. taurus Thomas, n. sp. Four new synonymies are proposed: L. catharinensis Kessel (=L. incisus Sharp), new synonym; L. similans Kessel (=L. incisus Sharp), new synonym; L. distinguendus Sharp (=L. megacephalus Grouvelle), new synonym, and L. chevrolati Grouvelle (=L. lecontei Grouvelle), new synonym. A key to the species ...

Start Scouting For Alfalfa Weevil With Updated Economic Thresholds, Erin W. Hodgson

Integrated Crop Management News

Even though it was an extremely cold winter, some alfalfa weevil adults are expected to survive. Adults will begin moving as soon as temperatures exceed 48°F and begin laying eggs in alfalfa. Alfalfa weevil eggs develop based on temperature, or accumulating degree days, and hatching can start around 200-300 degree days. Start scouting alfalfa fields south of Interstate 80 at 200 degree days and fields north of Interstate 80 at 250 degree days. Based on accumulated temperatures since January, weevils are active in southern Iowa now and scouting to monitor for larval activity should start in southwestern Iowa this ...

Predicted Mortality Of Bean Leaf Beetle Is Highest In 25 Years, Erin W. Hodgson, Adam J. Sisson

Integrated Crop Management News

Bean leaf beetle adults are susceptible to cold weather and most will die when the air temperature falls below -10°C. However, they have adapted to winter by protecting themselves under plant debris and loose soil. An overwintering survival model was developed by Lam and Pedigo from Iowa State University in 2000, and is helpful for predicting winter mortality based on accumulating subfreezing temperatures. Predicted mortality rates in Iowa were extremely high during the 2013-2014 winter and ranged from 89-99 percent (Fig. 1). Not surprisingly, central and northern Iowa experienced colder temperatures, and most of the bean leaf beetle adults ...

Eliminating Host-Mediated Effects Demonstrates Bt Maize Producing Cry1f Has No Adverse Effects On The Parasitoid Cotesia Marginiventris, Jun-Ce Tian, Xiang-Ping Wang, Li-Ping Long, Jörg Romeis, Steven E. Naranjo, Richard L. Hellmich, Anthony M. Shelton

Entomology Publications and Papers

The fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is an important pest of maize in the United States and many tropical areas in the western hemisphere. In 2001, Herculex I® (Cry1F) maize was commercially planted in the United States to control Lepidoptera, including S. frugiperda. In 2006, a population of S. frugiperda was discovered in Puerto Rico that had evolved resistance to Cry1F maize in the field, making it the first well-documented case of an insect with field resistance to a plant producing protein from Bacillus thuringiensis (Bt). Using this resistant population, we conducted tri-trophic studies with a ...

Short-Term, Mid-Term And Long-Term Effectiveness Of Silicosec® Against Cowpea Weevil, Adel Khashaveh

Adel Khashaveh

The insecticidal efficacy of SilicoSec®; a formulation of diatomaceous earth (DE); was evaluated against cowpea weevil (Callosobruchus maculatus) in cowpea seed at three storage periods: 0, 3 and 6 months. The DE formulation was applied at three dose rates: 0.1, 0.5 and 1 g/kg. The experiments carried out at 25 °C and 55% RH and continuous darkness. The mortality of C. maculatus adults was recorded 72 h after exposure to DE-treated seeds. After mortality count, all insects (dead and alive) were removed from vials and the vials were kept under same condition for a further 35 days ...

Molecular Evolution Of Glycoside Hydrolase Genes In The Western Corn Rootworm (Diabrotica Virgifera Virgifera), Seong-Il Eyun, Haichuan Wang, Yannick Pauchet, Richard H. Ffrench-Constant, Andrew K. Benson, Arnubio Valencia-Jimenez, Etsuko Moriyama, Blair Siegfried

Faculty Publications: Department of Entomology

Cellulose is an important nutritional resource for a number of insect herbivores. Digestion of cellulose and other polysaccharides in plant-based diets requires several types of enzymes including a number of glycoside hydrolase (GH) families. In a previous study, we showed that a single GH45 gene is present in the midgut tissue of the western corn rootworm, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae). However, the presence of multiple enzymes was also suggested by the lack of a significant biological response when the expression of the gene was silenced by RNA interference. In order to clarify the repertoire of cellulose-degrading enzymes and related ...