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Autoantibodies To The Ny-Eso-1 Tumor Antigen In Metastatic Melanoma: Sialylation Of The Fc Region Of Immunoglobulin G Induces Differential Expression Signatures Of Inflammatory Molecules During Dendritic Cell Differentiation And Maturation, Martin Oaks, Nathaniel Rein, John O. Richards, James Shaffer 2014 Aurora Health Care

Autoantibodies To The Ny-Eso-1 Tumor Antigen In Metastatic Melanoma: Sialylation Of The Fc Region Of Immunoglobulin G Induces Differential Expression Signatures Of Inflammatory Molecules During Dendritic Cell Differentiation And Maturation, Martin Oaks, Nathaniel Rein, John O. Richards, James Shaffer

Journal of Patient-Centered Research and Reviews

Purpose: We tested the hypothesis that different glycoforms of antibodies from patients with metastatic melanoma have different functional effects on human dendritic cell differentiation and maturation.

Methods: Antibodies to the cancer antigen NY-ESO-1 were affinity-purified from patients with melanoma and further fractionated into different glycoforms by lectin chromatography. Sialic acid-rich and sialic acid-poor fractions of these immunoglobulin G antibodies (IgG) were added to dendritic cell cultures during both differentiation and maturation, and the resulting cellular messenger RNA (mRNA) and culture supernatants were tested by microarray and enzyme-linked immunoassay for molecules related to inflammatory pathways.

Results: We identified unique mRNA and ...


Tattletales And T-Bow Update 20141018, George McNamara 2014 The University of Texas

Tattletales And T-Bow Update 20141018, George Mcnamara

George McNamara

Tattletales and T-Bow Update 20141018

http://works.bepress.com/gmcnamara/63

Tattletales is my concept to multiplex fluorescent protein biosensors ("Tattletales", which is also the overall concept name) and multicolor FP reporters ("T-Bow" for Rainbow T-cells and tumor cells) to study the physiology of live cells.

These are based on:

1. LacI-GFP :: LacO operator synthetic tandem repeat array (Robinett et al 1996 JCB, Fig 4A, http://jcb.rupress.org/content/135/6/1685.full.pdf )

2. the plethora of fluorescent protein biosensors - see page 1 of this "63" download site.

3. the many colors, and combinations of colors (CY11.5 ...


Validating Tbtif2-Interacting Candidates, Fan Wu, Jennifer Berkey, Joshua Hellsing, Nisha Thaker, Bibo Li 2014 Cleveland State University

Validating Tbtif2-Interacting Candidates, Fan Wu, Jennifer Berkey, Joshua Hellsing, Nisha Thaker, Bibo Li

Undergraduate Research Posters 2014

Transmitted by the tsetse fly, Trypanosoma brucei is a protozoan parasite that causes sleeping sickness in human and nagana in cattle. While infecting the bloodstream and central nervous system, T. brucei evades the immune system by altering its major surface antigen, Variant Surface Glycoproteins (VSGs), which forms a thick coat on its cell membrane. The expression sites for VSGs are at the sub-telomeric regions of T. brucei chromosomes. Telomeres, DNA-protein complexes located at the end of chromosomes, provide chromosome stability by preventing degradation of the chromosome ends. The telomere complex also regulates the sub-telomeric VSG expression and switching in T ...


Understanding Recurrent Disease: A Dynamical Systems Approach, Wenjing Zhang 2014 Western University

Understanding Recurrent Disease: A Dynamical Systems Approach, Wenjing Zhang

University of Western Ontario - Electronic Thesis and Dissertation Repository

Recurrent disease, characterized by repeated alternations between acute relapse and long re- mission, can be a feature of both common diseases, like ear infections, and serious chronic diseases, such as HIV infection or multiple sclerosis. Due to their poorly understood etiology and the resultant challenge for medical treatment and patient management, recurrent diseases attract much attention in clinical research and biomathematics. Previous studies of recurrence by biomathematicians mainly focus on in-host models and generate recurrent patterns by in- corporating forcing functions or stochastic elements. In this study, we investigate deterministic in-host models through the qualitative analysis of dynamical systems, to ...


Transcriptional Regulation Of Peptidylarginine Deiminase Type Iv: Implications For Rheumatoid Arthritis, Ali Abbas 2014 Western University

Transcriptional Regulation Of Peptidylarginine Deiminase Type Iv: Implications For Rheumatoid Arthritis, Ali Abbas

University of Western Ontario - Electronic Thesis and Dissertation Repository

High titers of anti-citrullinated protein antibodies have been detected in sera of rheumatoid arthritis (RA) patients, implicating citrullinating enzymes in the pathogenesis of RA. Peptidylarginine deiminase type IV (PAD4) is a member of the PAD family of enzymes that catalyze the post- translational modification of arginine to citrulline and has been linked with RA. However, little is known about its transcriptional regulation. Therefore, our aim was to determine how transcription of PAD4 is activated in the myeloid lineage. Using bioinformatics, a potential nuclear factor kappa B (NF-kB) binding site was identified on the PAD4 promoter. Luciferase assays were used to ...


Detection Of Microbial Catecholamines In A Model Wound Environment, Ryan F. Wasurick 2014 California Polytechnic State University

Detection Of Microbial Catecholamines In A Model Wound Environment, Ryan F. Wasurick

STEM Teacher and Researcher (STAR) Program Posters

Chronic wounds are defined as an injury that does not heal in a predictable pattern or time frame. The healing process is composed of specific stages and chronic wounds have been interrupted in one or more stages of this process. This project focuses on the bacterial component of an infected wound, specifically on the catecholamines influencing the bacteria in the wound site. The assay developed in this experiment is designed to optimally grow bacteria in a simulated eukaryotic wound environment, and evaluate biofilm expression and catecholamine production of the bacteria. Bacteria used in this study are Staphylococcus aureus and Pseudomonas ...


Potential Roles Of Peroxidases In Caenorhabditis Elegans Innate Immunity, George R. Tiller, george r. tiller 2014 Texas Medical Center Library

Potential Roles Of Peroxidases In Caenorhabditis Elegans Innate Immunity, George R. Tiller, George R. Tiller

UT GSBS Dissertations and Theses (Open Access)

The production of ROS (reactive oxygen species) in response to pathogen detection is a rapid, nonspecific response that is evolutionarily conserved from nematodes to humans. ROS serve as direct and indirect effectors of innate and adaptive immunity. In Caenorhabditis elegans, a ROS burst is observed during infection and is mediated by the dual oxidase BLI-3, which produces H2O2. RNAi (RNA interference) to reduce the amount of BLI-3 results in a significant increase in susceptibility to pathogens, suggesting BLI-3 has a role in the immune response. However, H2O2 by itself is not a potent antimicrobial ...


The Role Of The C5a Receptor In Host Defense Against Listeria Monocytogenes, Daniel Calame 2014 Texas Medical Center Library

The Role Of The C5a Receptor In Host Defense Against Listeria Monocytogenes, Daniel Calame

UT GSBS Dissertations and Theses (Open Access)

Listeria monocytogenes (Lm) is a major cause of mortality resulting from food poisoning in the United States. While the complement component C5 is known to be protective in listeriosis, it is unknown how its cleavage fragment C5a participates. Here we show in a model of systemic Lm infection that the C5a receptor is essential for host defense. C5aR-/- mice have reduced survival and increased bacterial burden in the liver and spleen in comparison to WT mice. Surprisingly, C5aR-/- mice also have a dramatic reduction in splenocyte numbers resulting from elevated cell death as indicated by TUNEL staining and caspase 3 ...


Oroya Fever And Verruga Peruana: Bartonelloses Unique To South America, Michael F. Minnick, Burt E. Anderson, Amorce Lima, James M. Battisti, Phillip G. Lawyer, Richard J. Birtles 2014 University of Montana

Oroya Fever And Verruga Peruana: Bartonelloses Unique To South America, Michael F. Minnick, Burt E. Anderson, Amorce Lima, James M. Battisti, Phillip G. Lawyer, Richard J. Birtles

Biological Sciences Faculty Publications

Bartonella bacilliformis is the bacterial agent of Carrión's disease and is presumed to be transmitted between humans by phlebotomine sand flies. Carrión's disease is endemic to high-altitude valleys of the South American Andes, and the first reported outbreak (1871) resulted in over 4,000 casualties. Since then, numerous outbreaks have been documented in endemic regions, and over the last two decades, outbreaks have occurred at atypical elevations, strongly suggesting that the area of endemicity is expanding. Approximately 1.7 million South Americans are estimated to be at risk in an area covering roughly 145,000 km2 of Ecuador ...


High Fat Diet Feeding Results In Gender Specific Steatohepatitis And Inflammasome Activation, Michal Ganz, Timea Csak, Gyongyi Szabo 2014 University of Massachusetts Medical School

High Fat Diet Feeding Results In Gender Specific Steatohepatitis And Inflammasome Activation, Michal Ganz, Timea Csak, Gyongyi Szabo

Gastroenterology Publications and Presentations

AIM: To develop an animal model that encompasses the different facets of non-alcoholic steatohepatitis (NASH), which has been a challenge.

METHODS: In this study, we used a high fat diet (HFD) feeding supplemented with fructose and sucrose in the water mimicking the high-fructose corn syrup that is abundant in the diet in the United States. We used C57Bl/6 wild-type mice for short and long-term feedings of 6 and 16 wk respectively, and evaluated the extent of liver damage, steatosis, and inflammasome activation. Our methods included histopathological analysis to assess liver damage and steatosis, which involved H and E and ...


The Hot Zone And The Cold War, Peter Nichols 2014 Colby College

The Hot Zone And The Cold War, Peter Nichols

Colby Magazine

It was a time when, in the nation's schools, drills for nuclear war were as frequent as fire drills- when Americans and their Soviet counterparts lived with a constant fear of imminent worldwide destruction. "You had a cultural mindset that was apocalyptic," said Robert S. Weisbrot, Christian A. Johnson Distinguished Teaching Professor of History at Colby, "that believed you could not guarantee that your wonderful suburban home and family and community...would survive ten more seconds."

"It was not simply a matter of two countries that are adversaries building up weapons," said Weisbrot. "Each saw the other as the ...


American Society Of Parasitologists Newsletter, V. 36, Nos. 1-2, Spring-Summer 2014, Scott Lyell Gardner 2014 University of Nebraska - Lincoln

American Society Of Parasitologists Newsletter, V. 36, Nos. 1-2, Spring-Summer 2014, Scott Lyell Gardner

Newsletter of the American Society of Parasitologists

Includes editor's note and contents, a report to the ASP by President Janovy, information about a new parasitology book, a report of the ASP Meeting in New Orleans, information about the transfer of the U.S. National Parasite Collection from the Agricultural Research Service to the Smithsonian Institution, and a list of credits and ASP Affiliates.


Understanding The Factors Affecting Microbiological Quality Of Wheat Milled Products: From Wheat Fields To Milling Operations, Luis E. Sabillón Galeas 2014 University of Nebraska - Lincoln

Understanding The Factors Affecting Microbiological Quality Of Wheat Milled Products: From Wheat Fields To Milling Operations, Luis E. Sabillón Galeas

Dissertations & Theses in Food Science and Technology

Although regarded as a low-risk commodity, wheat flour-based mixes have been implicated in several food safety incidents. The present thesis is a compilation of five scientific manuscripts on the effect of weather variation, milling steps and implementation of pre-milling interventions on the microbiological quality and safety of wheat and milled products. The first manuscript is a review of the microbiological quality and safety of wheat-based products. Despite the low water activity, wheat flour may harbor dormant but viable microorganisms, which could lead to safety concerns when flour is used to produce refrigerated dough products. The second manuscript illustrates the effect ...


Fty720 (Fingolimod) Provides Insight Into The Molecular Mechanisms Of Multiple Sclerosis, Madelyn Elizabeth Crawford 2014 University of Tennessee, Knoxville

Fty720 (Fingolimod) Provides Insight Into The Molecular Mechanisms Of Multiple Sclerosis, Madelyn Elizabeth Crawford

Pursuit - The Journal of Undergraduate Research at the University of Tennessee

Multiple sclerosis (MS) is a neurodegenerative disorder caused by a prolonged immune- mediated inflammatory response that targets myelin. Nearly all of the drugs approved for the treatment of MS are general immunosuppressants or only function in symptom management. The oral medication fingolimod, however, is reported to have direct therapeutic effects on cells of the central nervous system in addition to immunomodulatory functions. Fingolimod is known to interact with sphingosine-1-phosphate (S1P) receptors, and the most widely- accepted theory for its mechanism of action is functional antagonism of the receptor. This review examines significant neuromodulatory effects achieved by functional antagonism of the ...


Post-Apartheid South Africa’S Ultimate Challenge, Nahomie Julien 2014 Georgia State University

Post-Apartheid South Africa’S Ultimate Challenge, Nahomie Julien

DISCOVERY: Georgia State Honors College Undergraduate Research Journal

Throughout time, South Africa has experienced many upheavals, be they slavery and apartheid or natural, socioeconomic, and political misfortunes.Just after overcoming the oppression of Apartheid, South Africans have to face one of the deadliest illness in the world: HIV/AIDS. HIV/AIDS is currently the number one killer in South Africa(WHO, 2012). This pandemic further worsen the struggles of the nation, obstructing its educational, financial, and political recovery (Oglethorpe, & Gelman, (2008).; Weiser, et al., 2007.). This paper seeks to analyze how apartheid—or rather, its demise—contributed to the alarmingly rapid spread of this pandemic in South Africa. In so ...


Sub-Inhibitory Antibiotics Enhance Virulence, Persistence, And Pathogenesis Of Uropathogens, Lee W. Goneau 2014 Western University

Sub-Inhibitory Antibiotics Enhance Virulence, Persistence, And Pathogenesis Of Uropathogens, Lee W. Goneau

University of Western Ontario - Electronic Thesis and Dissertation Repository

In addition to their bactericidal effects, antibiotics are potent signal mediators at sub-inhibitory levels in the environment. The ability to modulate community structure in this niche raises concerns over their capacity to influence pathogenesis in patients during antibiotic therapy. This concept forms the basis of this thesis, and is explored using models of prophylactic therapy for recurrent urinary tract infection (UTI) management.

Sub-inhibitory ciprofloxacin, ampicillin, and gentamicin were found to augment virulence in vitro, increasing adherence and urothelial cell invasion in uropathogenic Escherichia coli (UPEC) and Staphylococcus saprophyticus. In addition, biofilm formation was increased, and swarming motility decreased. In UPEC ...


Tattletales And T-Bow Update 20140602mon, George McNamara 2014 The University of Texas

Tattletales And T-Bow Update 20140602mon, George Mcnamara

George McNamara

Tattletales and T-Bow Update 20140602Mon

http://works.bepress.com/gmcnamara/42

Please see also http://works.bepress.com/gmcnamara/26

Tattletales: multiplex fluorescent protein biosensors by spatial localization with TALE-FPs, Cas9-FPs, ZF-FPs, LacI-FPs, TetR-FPs, etc.

T-Bow: Rainbow T-cells and Tumor cells (and ES cells, iPS cells, other cells and organisms). You can think of this as "Brainbow meets TALENs/Cas9/ZFNs/other DNA sequence specific binding proteins".

If not familiar with Brainbow, see

http://en.wikipedia.org/wiki/Brainbow

If not familiar with TALENs, Cas9, etc, see

http://www.addgene.org/genome_engineering/

Big idea: localizing fluorescent proteins - and/or Nano-Lanterns ...


Crossreactive Epstein-Barr Virus (Ebv)-Influenza A Virus (Iav) Specific Cd8 Memory T Cells During Acute Symptomatic Iav Infection, Rabinarayan Mishra, Anna Gil, Nuray Aslan, Katherine Ruiz De Luzuriaga, Liisa K. Selin 2014 University of Massachusetts Medical School

Crossreactive Epstein-Barr Virus (Ebv)-Influenza A Virus (Iav) Specific Cd8 Memory T Cells During Acute Symptomatic Iav Infection, Rabinarayan Mishra, Anna Gil, Nuray Aslan, Katherine Ruiz De Luzuriaga, Liisa K. Selin

UMass Center for Clinical and Translational Science Research Retreat

We previously showed that crossreactivity is common between IAV and EBV in HLA-A2+ patients during infectious mononucleosis. IAV-M1-GIL58-66 specific CD8 T cells, along with expanded populations of IAV-M1-GIL58-66/EBV-BRLF-1109-117 -YVL and IAV-M1-GIL58-66/EBV-BMLF1280-288-GLC double-tetramer+ cells were detected directly ex-vivo in 5 HLA-A2+ patients. Altered IAV-M158-66, EBV-BRLF1119-117 and -BMLF1280-288 TCR repertoires were observed over the course of infection and in comparison to healthy donors. After culture, cells were sorted and analyzed by gene array in order to assess global changes in immune responses following different stimulations, either cognate or crossreactive, in different patient populations. M1-GIL and BRLF1-YVL specific cells had similar ...


Differential Gene Expression Analysis And Clinical Correlations Within Endemic Burkitt Lymphoma, Yasin Kaymaz, Cliff I. Oduor, John Vulule, Juliana A. Otieno, Ann M. Moormann, Jeffrey A. Bailey 2014 University of Massachusetts Medical School

Differential Gene Expression Analysis And Clinical Correlations Within Endemic Burkitt Lymphoma, Yasin Kaymaz, Cliff I. Oduor, John Vulule, Juliana A. Otieno, Ann M. Moormann, Jeffrey A. Bailey

UMass Center for Clinical and Translational Science Research Retreat

Endemic Burkitt lymphoma (eBL) is the most common pediatric cancer in equatorial Africa and is associated with malaria and Epstein-Barr virus co-infections. Molecular alterations within the eBL tumor genome and transcriptome have not been adequately investigated or compared to sporadic Burkitt lymphoma (sBL). Given that eBL has distinct clinical presentations in the jaw as opposed to the abdomen which are associated with survival, we hypothesize that transcriptome sequencing (RNA-seq) and potentially underlying genetic alterations will enhance our understanding of pathogenesis. Our results compare genome-wide RNA transcript abundances between eBL tumors from children (ages 6-7 yrs) with Stage I (Jaw tumor ...


Topical Anti-Tnf-Α Antibody Enhances Wound Healing In Diabetic Mice, Genevieve Broderick, Heather M. Strom, Ronald A. Ignotz 2014 University of Massachusetts Medical School

Topical Anti-Tnf-Α Antibody Enhances Wound Healing In Diabetic Mice, Genevieve Broderick, Heather M. Strom, Ronald A. Ignotz

UMass Center for Clinical and Translational Science Research Retreat

Chronic wounds such as venous leg ulcers and diabetic foot ulcers represent a significant health problem for which current treatment options are limited and not highly effective. These wounds have major impacts on the quality of life for affected individuals, frequently lead to amputation, and cost many thousands of dollars per year to treat. Assessment of wound fluid from such wounds indicates greatly elevated and persistently high levels of inflammatory cytokines, in particular Tumor Necrosis Factor-alpha (TNF-α). As such, these wounds are not in a physiologic state conducive to healing. Our study in diabetic mice (db/db) suggests that topical ...


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