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Laboratory and Basic Science Research Commons

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Elucidating Immune Signaling Of Influenza A Virus And Aspergillus Fumigatus Co-Infections Through Pioneered Model Development, Meagan Danyelle Rippee-Brooks 2019 Missouri State University

Elucidating Immune Signaling Of Influenza A Virus And Aspergillus Fumigatus Co-Infections Through Pioneered Model Development, Meagan Danyelle Rippee-Brooks

MSU Graduate Theses

Bacterial co-infections with influenza A virus (IAV) are extremely serious and life-threatening. However, there exists limited understanding about the importance of fungal infections with IAV. Clinical case reports indicate that fungal co-infections do occur and suggest the IAV pandemic of 2009 had a propensity to predispose patients to secondary fungal infections more than previous IAV strains. IAV-fungal co-infections are marked by high mortality rates of 47 to 61% in previously healthy individuals between the ages of 20 and 60. Yet, the variables involved in this co-infection remain undetermined. I achieved effective recapitulation of this co-infection using a C57Bl/6 murine ...


Examination Of The Role Of Neuroimmune Signaling In Drug Addiction, Catherine Levy 2019 University of Colorado, Boulder

Examination Of The Role Of Neuroimmune Signaling In Drug Addiction, Catherine Levy

Undergraduate Honors Theses

Cocaine and oxycodone addictions are chronic, relapsing disorders characterized by maladaptive patterns of drug seeking that create significant impairment for the user. While the homeostatic functions of neuroimmune cells such as microglia and astrocytes have been well characterized, little work has examined the structural and functional alterations of these neuroglial cells following repeated drug intake. The first experiment of this study used male and female Sprague-Dawley rats to examine sex differences in microglial cells following repeated cocaine intake. The second experiment used a rodent model of oxycodone self-administration to test the ability of ibudilast, a non-selective anti-inflammatory drug, to reduce ...


Modified Open-Field Test With Odor Search Stimulus: Anticipated Canine Motivation And Behavioral Outcomes Between Anxious And Non-Anxious Dogs, Savanna Bergeron 2019 Iowa State University

Modified Open-Field Test With Odor Search Stimulus: Anticipated Canine Motivation And Behavioral Outcomes Between Anxious And Non-Anxious Dogs, Savanna Bergeron

Creative Components

Fear is an emotion that is necessary to survive, but when it’s prolonged and frequent, it can cause suffering in both animals and humans. Fear and anxiety are interrelated; therefore, fear can cause anxiety and anxiety can cause fear. Treatments for anxiety behaviors are currently an ongoing process, in order to improve the mental health of the animal. This review is focused on trying to understand anxiety behaviors through the use of a modified open-field test. More specifically, an odor search stimulus is used in conjunction with dogs on L-DOPA treatments. Previous animal models, particularly the mouse and rat ...


Smart Sheep Need More Protection, Michael L. Woodruff 2019 East Tennessee State University

Smart Sheep Need More Protection, Michael L. Woodruff

Animal Sentience

The target article unequivocally establishes that sheep are far more intelligent and cognitively sophisticated than is generally acknowledged. For this reason, the authors advocate for significantly more stringent regulation of agricultural and research practices when sheep are used. I briefly review the existing US regulations governing the use of sheep in research and discuss the extent to which they are applied to sheep. I then discuss weaknesses in the current regulations, concluding that they should be changed to mandate housing all research animals in environments that accommodate the psychosocial needs of each species.


Neuronal And Glial Expression Of Glun2c And Glun2d Nmda Receptor Subunit Mrna In Different Regions Of The Mouse Forebrain, Hassan Alsaad 2018 University of Nebraska Medical Center

Neuronal And Glial Expression Of Glun2c And Glun2d Nmda Receptor Subunit Mrna In Different Regions Of The Mouse Forebrain, Hassan Alsaad

Theses & Dissertations

N-methyl-D-aspartate (NMDA) receptors are a subtype of glutamate receptors that are widely expressed in the brain, where they mediate critical functions. While the actions of the predominate GluN2 NMDAR subunits, GluN2A and GluN2B are relatively well understood, the function of GluN2C and GluN2D in the telencephalon is largely unknown. To better understand the possible role of GluN2C and GluN2D subunits, we used fluorescence in situ hybridization together with multiple cell markers to define the distribution and type of cells expressing GluN2C and GluN2D mRNA. Also, a GluN2C-KO mouse was used as a negative control.

GluN2C mRNA expression was only found ...


Alternatives To Laboratory Animals: Definition And Discussion, Andrew N. Rowan 2018 The Institute for the Study of Animal Problems

Alternatives To Laboratory Animals: Definition And Discussion, Andrew N. Rowan

Andrew N. Rowan, DPhil

The origins of the concept of "alternatives" to the use of animals in research may be traced back to the 1800's and the furore about using live animals in surgical and other experiments. Some of the animal protection societies in England were prepared to accept animal experimentation provided it was performed under anesthesia. Even Dr. Marshall Hall, who championed the spread of experimental medicine in 19th century England, considered it necessary to control and prevent unwarranted, inept and cruel experimentation (French, 1975). The concept of alternatives has developed in the 20th century to encompass not only the reduction (prevention ...


Behavioral Insights Into Nociceptor Function: A Systematic Approach To Understanding Postsurgical And Neuropathic Pain Mechanisms In Rats, Max Odem 2018 The Univesity of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences

Behavioral Insights Into Nociceptor Function: A Systematic Approach To Understanding Postsurgical And Neuropathic Pain Mechanisms In Rats, Max Odem

UT GSBS Dissertations and Theses (Open Access)

Postsurgical and neuropathic pain are each clinically common, and often associated with ongoing pain. Ongoing pain has been linked to ongoing activity (OA) in human C-fiber nociceptors. Preclinical studies using rodent neuropathic models have concentrated on allodynia driven by OA generated in non-nociceptive Aβ fibers, but little attention has been paid to postsurgical pain in sham controls or to C-fiber nociceptor OA promoting ongoing pain.

Operant assays that reveal negative motivational and cognitive aspects of voluntary pain-related behavior may be particularly sensitive to pain-related alterations. In the mechanical conflict (MC) test, rodents can freely choose to escape from a brightly ...


22 - Bugs In Bugs, Josemaria Garcia 2018 University of North Georgia

22 - Bugs In Bugs, Josemaria Garcia

Georgia Undergraduate Research Conference (GURC)

The primary purpose of the Bugs in Bugs project is to measure a student’s ability to go complete a research report and to observe demonstrations of key fundamental research aspects such as understanding scientific works, collecting and entering data, analyzing and creating graphs, and overall how to write a lab report. Working with Principles of Biology II and Microbiology classes, students were provided with a topic and methodology respective to their class; however, groups within the classes had the freedom to create their own hypotheses and theses, choose how to collect and interpret data, make charts and graphs, and ...


Adapting Cell-Free Protein Synthesis As A Platform Technology For Education, Grace W. Chu, Max Z. Levine, Nicole E. Gregorio, Javin P. Oza 2018 California State University, Los Angeles

Adapting Cell-Free Protein Synthesis As A Platform Technology For Education, Grace W. Chu, Max Z. Levine, Nicole E. Gregorio, Javin P. Oza

STAR (STEM Teacher and Researcher) Presentations

Cell-free protein synthesis (CFPS) has emerged as an enabling biotechnology for research and biomanufacturing as it allows for the production of protein without the need for a living cell. Applications of CFPS include the construction of libraries for functional genomics and structural biology, the production of personalized medicine, and the expression of virus-like particles. The absence of a cell wall provides an open platform for direct manipulation of the reaction conditions and biological machinery. This project focuses on adapting the CFPS biotechnology to the classroom, making a hands-on bioengineering approach to learning protein synthesis accessible to students grades K-16 through ...


Personal Safety Culture: A New Measure For General Aviation Pilots, Bradley S. Baugh, Kimberly M. Bracewell, Urara Takano, Mattie N. Milner, Emily C. Anania, Nadine K. Ragbir, Mudhur Bharat Gupta, Diego M. Garcia, Drishti O. Valecha, Daniel A. Marte, Scott R. Winter, Stephen Rice 2018 Embry-Riddle Aeronautical University

Personal Safety Culture: A New Measure For General Aviation Pilots, Bradley S. Baugh, Kimberly M. Bracewell, Urara Takano, Mattie N. Milner, Emily C. Anania, Nadine K. Ragbir, Mudhur Bharat Gupta, Diego M. Garcia, Drishti O. Valecha, Daniel A. Marte, Scott R. Winter, Stephen Rice

National Training Aircraft Symposium (NTAS)

Safety culture has been a subject of research for over three decades and is now widely accepted as a critical component of organizational safety programs both domestically and internationally. Through the development of a healthy safety culture, aviation organizations can improve safety processes, reduce mishaps, and mitigate risk more effectively. This is done through the holistic team efforts of an organization’s members and the organization’s leadership. How about aviators who are not part of an organization? Is it possible to identify a personal safety culture defined outside of the traditional organization? And, is it possible to create an ...


Promoting Adherence To Best Practice Related To Urine Reflex To Culture Testing, Maykel Verdecia Alonso BA,RN-CPHN 2018 The University of San Francisco

Promoting Adherence To Best Practice Related To Urine Reflex To Culture Testing, Maykel Verdecia Alonso Ba,Rn-Cphn

Nursing and Health Professions Faculty Research and Publications

Over a two week period, the infection control nurse, from a trauma designated facility located in norther California, planned a practice improvement project in collaboration with the laboratory microsystem to educate referring physicians and increase adherence to the Centers for Disease Control (CDC) protocol, which delineates recommended best practices related to urine reflex to culture testing. Urinalysis (UA) is a test that triggers a reflex urine culture when pyuria is present. Frequent urine culturing without the presence of pyuria can cause unnecessary treatment with antimicrobials. From January 2017 to December 2017, 10% of UAs from 200 urine samples from asymptomatic ...


Computational Analysis Of Genomic Variants Affecting Predicted Microrna:Target Interactions In Prostate Cancer., Angélica Paola Hernández Pérez 2018 Keck Graduate Institute, KGI

Computational Analysis Of Genomic Variants Affecting Predicted Microrna:Target Interactions In Prostate Cancer., Angélica Paola Hernández Pérez

KGI Theses and Dissertations

Prostate cancer (PCa) is the most common cancer of men in the United States and is third only to lung and colon as a cause of cancer death. Clinical behavior of the disease is variable and the combination of prostate-specific antigen (PSA) screening and Gleason score staging are currently the best available molecular and pathology tools to predict outcomes. Cancer biology research establishes microRNAs (miRNAs) as key molecular components in both normal and pathological states. Thus, elucidating miRNAs perturbed by genomic alterations will expand our understanding of the molecular taxonomy of PCa with the aim to complement current practices in ...


Investigation Of Zebrafish Larvae Behavior As Precursor For Suborbital Flights: Feasibility Study, Pedro Llanos, Kristina Andrijauskaite, Mark Rubinstein, Sherine S.L. Chan 2018 Embry-Riddle Aeronautical University - Daytona Beach

Investigation Of Zebrafish Larvae Behavior As Precursor For Suborbital Flights: Feasibility Study, Pedro Llanos, Kristina Andrijauskaite, Mark Rubinstein, Sherine S.L. Chan

Pedro J. Llanos (www.AstronauticsLlanos.com)


Suborbital spaceflights, carrying scientific payloads, allow scientists not only to test the feasibility of their payloads, but they also provide the basis for refining scientific hypotheses to be later tested on the International Space Station (ISS). Therefore, it is essential to establish robust pre-flight procedures in order to take advantage of this unique research platform to facilitate payload delivery. In the present study, we assessed zebrafish larvae behavior as a precursor for the future suborbital spaceflight involving research on the musculoskeletal system. Zebrafish larvae were exposed to the same physiological stressors they would encounter during suborbital spaceflight: alterations in light ...


Importance Of Welfare And Ethics Competence Regarding Animals Kept For Scientific Purposes To Veterinary Students In Australia And New Zealand, Teresa Collins, Amelia Cornish, Jennifer Hood, Chris Degeling, Andrew D. Fisher, Rafael Freire, Susan J. Hazel, Jane Johnson, Jennifer K.F. Lloyd, Clive J.C. Phillips, Vicky Tzioumis, Paul D. McGreevy 2018 Murdoch University

Importance Of Welfare And Ethics Competence Regarding Animals Kept For Scientific Purposes To Veterinary Students In Australia And New Zealand, Teresa Collins, Amelia Cornish, Jennifer Hood, Chris Degeling, Andrew D. Fisher, Rafael Freire, Susan J. Hazel, Jane Johnson, Jennifer K.F. Lloyd, Clive J.C. Phillips, Vicky Tzioumis, Paul D. Mcgreevy

Professional Science Research Ethics Collection

Veterinarians are in a strong position of social influence on animal-related issues. Hence, veterinary schools have an opportunity to raise animal health and welfare standards by improving veterinary students’ animal welfare and ethics (AWE) education, including that related to animals used for scientific purposes. A survey of 818 students in the early, mid, and senior stages of their courses at all eight veterinary schools across Australia and New Zealand was undertaken on their first day of practice (or Day One Competences) to explore how veterinary students viewed the importance of their competence in the management of welfare and ethical decision-making ...


Bioethics (Gcsu), Kasey Karen, Jennifer Townes 2018 Georgia College and State University

Bioethics (Gcsu), Kasey Karen, Jennifer Townes

Biological Sciences Grants Collections

This Grants Collection for Bioethics was created under a Round Ten ALG Textbook Transformation Grant.

Affordable Learning Georgia Grants Collections are intended to provide faculty with the frameworks to quickly implement or revise the same materials as a Textbook Transformation Grants team, along with the aims and lessons learned from project teams during the implementation process.

Documents are in .pdf format, with a separate .docx (Word) version available for download. Each collection contains the following materials:

  • Linked Syllabus
  • Initial Proposal
  • Final Report


Spatial And Temporal Patterns Of Arctic Nearshore Fish Community And Food Web Structures, Mark B. Barton 2018 Florida International University

Spatial And Temporal Patterns Of Arctic Nearshore Fish Community And Food Web Structures, Mark B. Barton

FIU Electronic Theses and Dissertations

Climate change and increasing anthropogenic activities are causing rapid changes to environmental and ecological processes in the Arctic Ocean. To better understand these changes, scientists have increased research efforts in these regions, but to date the number of studies on Arctic nearshore habitats are lacking. My dissertation responds to the paucity of information and investigates patterns in Arctic nearshore fish communities and food webs to gain insight to how these ecosystems may shift as these changes continue. I used multivariate statistical analysis to examine patterns in community structure and composition to determine that Arctic nearshore fish communities are largely driven ...


Mapping Molecular Datasets Back To The Brain Regions They Are Extracted From: Remembering The Native Countries Of Hypothalamic Expatriates And Refugees, Arshad M. Khan, Alice H. Grant, Anais Martinez, Gully APC Burns, Brendan S. Thatcher, Vishwanath T. Anekonda, Benjamin W. Thompson, Zachary S. Roberts, Daniel H. Moralejo, James E. Blevins 2018 University of Texas at El Paso

Mapping Molecular Datasets Back To The Brain Regions They Are Extracted From: Remembering The Native Countries Of Hypothalamic Expatriates And Refugees, Arshad M. Khan, Alice H. Grant, Anais Martinez, Gully Apc Burns, Brendan S. Thatcher, Vishwanath T. Anekonda, Benjamin W. Thompson, Zachary S. Roberts, Daniel H. Moralejo, James E. Blevins

Arshad M. Khan, Ph.D.

This article, which includes novel unpublished data along with commentary and analysis,
focuses on approaches to link transcriptomic, proteomic, and peptidomic datasets mined from
brain tissue to the original locations within the brain that they are derived from using digital atlas
mapping techniques. We use, as an example, the transcriptomic, proteomic and peptidomic
analyses conducted in the mammalian hypothalamus. Following a brief historical overview, we
highlight studies that have mined biochemical and molecular information from the hypothalamus
and then lay out a strategy for how these data can be linked spatially to the mapped locations in a
canonical brain atlas ...


Biological Soil Crusts In A Northeastern Pine Barren: Composition And Ecological Effects, Jessica Gilbert 2018 Union College - Schenectady, NY

Biological Soil Crusts In A Northeastern Pine Barren: Composition And Ecological Effects, Jessica Gilbert

Honors Theses

Biological soil crusts (BSCs), otherwise known as cryptogamic soil crusts, biocrusts, or cyanobacterial crusts, are soil aggregations hosting diverse biotic communities. They are composed of cyanobacteria and algae, and generally have a covering of moss and/or lichen. BSCs are typically found in arid to semi-arid regions throughout the world, and are integral soil stabilizers, moisture retainers, and nitrogen fixers in these communities. Along with these factors, BSCs are able to impact germination and establishment of plants, either as an accompanying influence, or direct result of those listed above. BSCs have yet to be formally described in the inland northeastern ...


Light-Induced Expression Of A Blue Coral Protein In An Industrial Fungus, Lindsay Tomczak 2018 Union College

Light-Induced Expression Of A Blue Coral Protein In An Industrial Fungus, Lindsay Tomczak

Honors Theses

The ultimate goal of this research project is to improve the growth and structural characteristics of an Ecovative Design LLC (Green Island, NY) production strain to produce commercial biomaterials for packaging. These biomaterials are produced from renewable resources and can be easily broken down after they fulfill their purpose, unlike the commonly used materials today (such as Styrofoam). In an effort to quantify the light-reactivity of the fungus, a codon-optimized DNA sequence coding a blue chromoprotein was introduced and utilized as a visual reporter gene. Transcriptional controlling sequences were identified from orthologs to specific light-regulated genes and were combined with ...


Analysis Of Metallic Components Of Gsr From Various Types Of Ammunition And Firearms Utilizing An Sem-Edx, Jenna A. Campbell 2018 Duquesne University

Analysis Of Metallic Components Of Gsr From Various Types Of Ammunition And Firearms Utilizing An Sem-Edx, Jenna A. Campbell

Graduate Student Research Symposium

Gunshot residue (GSR) is a chemical reaction that occurs when a firearm is discharged, which emits a cloud of gases from the barrel of the gun. It consists of both the burned and unburned propellant and primer components. These components are either organic or inorganic in nature, respectively. The organic components are located in the gunpowder part of the bullet, while the inorganic components are located in the primer part of the bullet. The primer consists of lead, barium, and antimony. These elements are considered heavy metals. Additionally, lead, barium, and antimony are the three key elements found in the ...


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