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Evaluation Of Surgical Methods For Treatment Of Cubital Tunnel Syndrome - Statistical Perspectives, Ahmed Mohammed Morsy 2017 Assiut University, South Egypt Cancer Institute, Pediatric Oncology Department

Evaluation Of Surgical Methods For Treatment Of Cubital Tunnel Syndrome - Statistical Perspectives, Ahmed Mohammed Morsy

Dr/ Ahmed Mohammed Morsy

This commentary addresses strengths and weaknesses of the clinical trial study protocol designed by Liu et al., in particular with regard of the methodology, taking into consideration the commentary article "Assessing surgical methods for treatment of cubital tunnel syndrome - which is the best?" Our commentary emphasizes the importance of adjustment for known prognostic covariates, such as duration of symptoms and advanced age that have been negatively correlated with outcomes in previous studies. Subgroup analysis for the treatment groups of interest, namely moderate and severe cubital tunnel syndrome, which have previously shown conflicting differences in efficacy of surgical options is highly ...


Amelioration Of Prenatal Alcohol Effects By Environmental Enrichment In A Mouse Model Of Fasd, Aniruddho Chokroborty-Hoque 2017 The University of Western Ontario

Amelioration Of Prenatal Alcohol Effects By Environmental Enrichment In A Mouse Model Of Fasd, Aniruddho Chokroborty-Hoque

Electronic Thesis and Dissertation Repository

Maternal alcohol consumption during pregnancy results in a spectrum of behavioural and cognitive deficits collectively known as Fetal Alcohol Spectrum Disorders (FASD). Currently, little is know about if and how the external environment may modulate these deficits. I have used C57BL/6 mice to study this interaction between prenatal alcohol exposure and the postnatal environment. Alcohol exposure during synaptogenesis produces high levels of anxiety-like traits and decreased memory performance. Alcohol-exposed mice (and matched unexposed controls) were put in 'environmentally-enriched' conditions of voluntary exercise, physical activities and cognitive stimulation to ascertain the effects of a positive postnatal environment. The results show ...


T-Time: A Data Repository Of T Cell And Calcium Release-Activated Calcium Channel Activation Imagery, Cody Arbuckle, Milton L. Greenberg, Adrienne Bergh, Rene German, Nick Sirago, Erik J. Linstead 2017 Chapman University

T-Time: A Data Repository Of T Cell And Calcium Release-Activated Calcium Channel Activation Imagery, Cody Arbuckle, Milton L. Greenberg, Adrienne Bergh, Rene German, Nick Sirago, Erik J. Linstead

Mathematics, Physics, and Computer Science Faculty Articles and Research

BACKGROUND: A fundamental understanding of live-cell dynamics is necessary in order to advance scientific techniques and personalized medicine. For this understanding to be possible, image processing techniques, probes, tracking algorithms and many other methodologies must be improved. Currently there are no large open-source datasets containing live-cell imaging to act as a standard for the community. As a result, researchers cannot evaluate their methodologies on an independent benchmark or leverage such a dataset to formulate scientific questions.

FINDINGS: Here we present T-Time, the largest free and publicly available data set of T cell phase contrast imagery designed with the intention of ...


A Review Of The Institute Of Medicine’S Analysis Of Using Chimpanzees In Biomedical Research, Robert C. Jones, Ray Greek 2017 California State University, Chico

A Review Of The Institute Of Medicine’S Analysis Of Using Chimpanzees In Biomedical Research, Robert C. Jones, Ray Greek

Robert C. Jones, Ph.D.

We argue that the recommendations made by the Institute of Medicine’s 2011 report, Chimpanzees in Biomedical and Behavioral Research: Assessing the Necessity, are methodologically and ethically confused. We argue that a proper understanding of evolution and complexity theory in terms of the science and ethics of using chimpanzees in biomedical research would have had led the committee to recommend not merely limiting but eliminating the use of chimpanzees in biomedical research. Specifically, we argue that a proper understanding of the difference between the gross level of examination of species and examinations on finer levels can shed light on important ...


Transcriptomic Differentiation Underlying Marine‐To‐Freshwater Transitions In The South American Silversides Odontesthes Argentinensis And O. Bonariensis (Atheriniformes), Lily Hughes, Gustavo Somoza, Bryan Nguyen, James Bernot, Mariano Gonzalez-Castro, Juan Martin Diaz de Astarloa, Guillermo Orti 2017 George Washington University

Transcriptomic Differentiation Underlying Marine‐To‐Freshwater Transitions In The South American Silversides Odontesthes Argentinensis And O. Bonariensis (Atheriniformes), Lily Hughes, Gustavo Somoza, Bryan Nguyen, James Bernot, Mariano Gonzalez-Castro, Juan Martin Diaz De Astarloa, Guillermo Orti

Computational Biology Institute

Salinity gradients are critical habitat determinants for freshwater organisms. Silverside fishes in the genus Odontesthes have recently and repeatedly transitioned from marine to freshwater habitats, overcoming a strong ecological barrier. Genomic and transcriptomic changes involved in this kind of transition are only known for a few model species. We present new data and analyses of gene expression and microbiome composition in the gills of two closely related silverside species, marine O. argentinensis and freshwater O. bonariensis and find more than three thousand transcripts differentially expressed, with osmoregulatory/ion transport genes and immune genes showing very different expression patterns across species ...


Ivd Formation In Monodelphis Domestica, Emma C.B. Gray, Jen Maier, Karen Sears 2017 Parkland College

Ivd Formation In Monodelphis Domestica, Emma C.B. Gray, Jen Maier, Karen Sears

Phenotypic Plasticity Research Experience for Community College Students

The research summarized on this poster supports the use of the gray short-tailed opossum (Monodelphis domestica) as a lab animal for research into invertebral disc formation and degeneration. Preliminary data suggests that some genes involved in the formation and maintenance of the notochord in mice are the same in the short-tailed opossum. Data also suggests the ossification of the vertebral column of pups proceeds anteriorly to posteriorly and that much of the maturation of the nucleus puposus happens in the first ten days.


Letter -- Re: Biomedical Experiments Involving Non-Human Primates, Jarrod Bailey 2017 Cruelty Free International

Letter -- Re: Biomedical Experiments Involving Non-Human Primates, Jarrod Bailey

Jarrod Bailey, Ph.D.

One of the main motivations for my 2016 review was my frustration at the repeated, unsound case put forth by many of those who advocate for continued, and greater, invasive neuroscience on our primate cousins. In my view, this case is multiply and seriously flawed. It overlooks the opposition of the public to animal experiments, in particular to NHP neuroscience; it skews the balance of associated harms and proposed benefits by understating the former and overstating the latter; it assumes a default human relevance without critically questioning this assumption and without appreciating the abundant evidence against it; it plays down ...


Analysis Of Population Structure In A California Newt (Taricha Torosa) Metapopulation, Jessica Vincent 2017 Western Kentucky University

Analysis Of Population Structure In A California Newt (Taricha Torosa) Metapopulation, Jessica Vincent

Honors College Capstone Experience/Thesis Projects

As anthropogenic influences take an ever-increasing toll on the environment, understanding how environmental change affects species is paramount. Concern regarding decline in amphibian populations has spurred research examining the effects of habitat change on the dynamics of populations at landscape levels. One important goal is to understand how gene flow among populations is affected by changes in habitat. Biologists need to consider the relationship between gene flow and habitat alterations so that movements among individual breeding ponds can be maintained over time, reducing risk of local extinction events. This study focuses on patterns of gene flow among thirteen populations of ...


Finding A Link Between Circadian Rhythms And The Immune System Of Captive Zebra Finches (Taeniopygia Guttata), Amber Bishop 2017 Western Kentucky University

Finding A Link Between Circadian Rhythms And The Immune System Of Captive Zebra Finches (Taeniopygia Guttata), Amber Bishop

Honors College Capstone Experience/Thesis Projects

Circadian rhythms are commonplace in organisms and are normally controlled by a master clock. More recent evidence suggests that autonomous clocks operate in various systems in the body, including the immune system. This study looks for such a connection between the circadian rhythm and the immune system. In this study, captive zebra finches (Taeniopygia guttata) were exposed to different light and dark cycles and blood samples were taken every six hours. Birds were exposed to 12 hours of light and then 12 hours of darkness (LD), 24 hours of darkness (DD), or 48 hours of DD. After collecting whole blood ...


Evaluating The Social Behavior And Activity Patterns Of Clouded Leopards (Neofelis Nebulosa) At The Nashville Zoo: Research And Literature Review, Meghan Ryckeley 2017 Western Kentucky University

Evaluating The Social Behavior And Activity Patterns Of Clouded Leopards (Neofelis Nebulosa) At The Nashville Zoo: Research And Literature Review, Meghan Ryckeley

Honors College Capstone Experience/Thesis Projects

The behavior of the elusive clouded leopard (Neofelis nebulosa) is not well understood. In captivity, they are prone to exhibit stress-related behaviors and are often aggressive to conspecifics when paired. The present study built on past research by examining clouded leopards at the Nashville Zoo at Grassmere (NZG) to determine their activity patterns and temperament. Twelve leopards were exposed to familiar and unfamiliar objects and people, as well as their reflection in a mirror over nine trials. The males were often more reactive than the females, and the 5-year olds more reactive than their younger counterparts. The mirror image and ...


Detection Of Tetracyclines In An Anaerobic Waste Digester Using Solid Phase Extraction And High-Performance Liquid Chromatography Mass Spectrometry, Courtney Cruse 2017 Western Kentucky University

Detection Of Tetracyclines In An Anaerobic Waste Digester Using Solid Phase Extraction And High-Performance Liquid Chromatography Mass Spectrometry, Courtney Cruse

Honors College Capstone Experience/Thesis Projects

Antibiotics are introduced to livestock to encourage growth and for the treatment of diseases. These antibiotics are not completely metabolized by swine, and thus these antibiotics are excreted with their waste. This poses a potential risk to human health as these antibiotics, a potential link to antibiotic resistant bacteria, then enter the surface water, ground water, and soil. In collaboration with the US Department of Agriculture (USDA) in Bowling Green, Kentucky, this research is concerned with analyzing the degradation of tetracyclines in swine waste from an anaerobic digester. Waste samples obtained from a digester and swine waste at the USDA ...


Growth And Survival Of Salamanders Exposed To Different Formulations Of Glyphosate-Based Herbicide, Jessica Johnson 2017 Western Kentucky University

Growth And Survival Of Salamanders Exposed To Different Formulations Of Glyphosate-Based Herbicide, Jessica Johnson

Honors College Capstone Experience/Thesis Projects

Amphibian populations have been experiencing rapid declines worldwide in the past few decades. There are many proposed causations, including the use of agricultural chemicals such as herbicides. Glyphosate based herbicides are one of the most widely used herbicides. This study looks at the effects of different brands of glyphosate-based herbicides, including those intended for aquatic use, on the survival and growth of axolotl salamander larvae. Out of four brands of glyphosate herbicide (Aquamaster, Aquaneat, Helosate plus, and Roundup Pro), the survival rates of Roundup Pro were the lowest. Most mortality occurred between the 3 mg/L and 6 mg/L ...


Characterization Of An 18f-Growth Hormone Secretagogue Probe For Positron Emission Tomography Imaging Of The Growth Hormone Secretagogue Receptor, Ahmed Abbas 2017 The University of Western Ontario

Characterization Of An 18f-Growth Hormone Secretagogue Probe For Positron Emission Tomography Imaging Of The Growth Hormone Secretagogue Receptor, Ahmed Abbas

Electronic Thesis and Dissertation Repository

Cardiovascular disease affects 1.6 million Canadians, of whom one-third have heart failure (HF). HF is diagnosed by imaging investigations and detection of circulating biomarkers. Most of the current imaging strategies study morphologic and gross functional changes, but fall short of imaging molecular abnormalities associated with HF. Biomarkers offer molecular targets; however, clinical biomarkers circulate systemically and are not cardiac-specific. Thus, there is critical need for a biomarker that is endogenous to myocardial tissues. The growth hormone secretagogue receptor 1a (GHSR1a), which binds the hormone ghrelin, is expressed by cardiomyocytes and is elevated in HF patients. This study characterized the ...


Rnaseq Analysis Of The Drosophila Response To The Entomopathogenic Nematode Steinernema., Shruti Yadav, Sean Daugherty, Amol Carl Shetty, Ioannis Eleftherianos 2017 George Washington University

Rnaseq Analysis Of The Drosophila Response To The Entomopathogenic Nematode Steinernema., Shruti Yadav, Sean Daugherty, Amol Carl Shetty, Ioannis Eleftherianos

Computational Biology Institute

Drosophila melanogaster is an outstanding model to study the molecular and functional basis of host-pathogen interactions. Currently, our knowledge of microbial infections in D. melanogaster is well understood; however, the response of flies to nematode infections is still in its infancy. Here, we have used the potent parasitic nematode Steinernema carpocapsae, which lives in mutualism with its endosymbiotic bacteria Xenorhabdus nematophila, to examine the transcriptomic basis of the interaction between D. melanogaster and entomopathogenic nematodes. We have employed next-generation RNA sequencing (RNAseq) to investigate the transcriptomic profile of D. melanogaster larvae in response to infection by S. carpocapsae symbiotic (carrying ...


Linking Large Scale Ocean-Atmospheric Patterns With Recruitment In Kellet’S Whelk (Kelletia Kelletii), Katherine N. Rodriguez 2017 California Polytechnic State University, San Luis Obispo

Linking Large Scale Ocean-Atmospheric Patterns With Recruitment In Kellet’S Whelk (Kelletia Kelletii), Katherine N. Rodriguez

Biological Sciences

Global warming influences the biogeography of many marine and terrestrial species. Understanding species range shifts is ecologically and socioeconomically important when guiding management decisions for ecosystems exposed to a rapidly changing climate. In this natural experiment, I follow Danielle Zacherl’s methods (Zacherl et al. 2003) to study the effects of large-scale ocean-atmospheric patterns on recruitment of a marine snail, Kellet’s whelk (Kelletia kelletii); these organisms recently expanded their range North past the geographic barrier of Point Conception to Monterey, CA, USA. I use shell length data collected at 32 subtidal rocky reef kelp forest sites in 2015 (before ...


The Effect Of Resource Quality And Species Interactions On The Colonization Behavior Of The Black Blow Fly, Phormia Regina (Meigen), Melissa N. Branker 2017 CUNY John Jay College

The Effect Of Resource Quality And Species Interactions On The Colonization Behavior Of The Black Blow Fly, Phormia Regina (Meigen), Melissa N. Branker

Student Theses

During decomposition, there are many different insects groups that utilize carrion as a resource. In particular, blow flies (Order: Diptera; Family: Calliphoridae) are considered to be a forensically important family due to their ability to rapidly locate and colonize a carrion resource. As a result, blow flies are commonly used as indicator species in PMI estimations. However, recent research indicates that the colonization behavior of these species can be influenced by a variety of abiotic and biotic factors. In this study, the effects of arrival order, resource quality and bacterial or species cues on the oviposition behavior of Phormia regina ...


Investigation Into The Mechanism Of Ion Transport In Rio Negro Characiformes, Vineza D. Reduta 2017 University of San Diego

Investigation Into The Mechanism Of Ion Transport In Rio Negro Characiformes, Vineza D. Reduta

Undergraduate Honors Theses

Four fish species within the Characiform Order [(Rosy tetras (Hyphessobrycon rosaceus), Serpae tetras (Hyphessobrycon eques), Emperor tetras (Nematobrycon palmeri) and Penguin tetras (Thayeria obliqua)] are native to the ion-poor, acidic waters of the Rio Negro. In this paper, we focus on Wood’s revised Na+/NH4+ exchange model to gain better insight into the mechanisms of ion transport within the Characids. We exposed each species to two experimental treatments: (i) 1mM ammonia (high external ammonia [HEA]), to test coupling of Na+ uptake and ammonia excretion, and (ii) 100μM Acetazolamide (carbonic anhydrase inhibitor), to test coupling of Na+ uptake and ...


Estimation Of The True Evolutionary Distance Under The Fragile Breakage Model., Nikita Alexeev, Max A Alekseyev 2017 George Washington University

Estimation Of The True Evolutionary Distance Under The Fragile Breakage Model., Nikita Alexeev, Max A Alekseyev

Computational Biology Institute

BACKGROUND: The ability to estimate the evolutionary distance between extant genomes plays a crucial role in many phylogenomic studies. Often such estimation is based on the parsimony assumption, implying that the distance between two genomes can be estimated as the rearrangement distance equal the minimal number of genome rearrangements required to transform one genome into the other. However, in reality the parsimony assumption may not always hold, emphasizing the need for estimation that does not rely on the rearrangement distance. The distance that accounts for the actual (rather than minimal) number of rearrangements between two genomes is often referred to ...


Mitigating The Conflict Between Pitfall-Trap Sampling And Conservation Of Terrestrial Subterranean Communities In Caves, Peter Kozel, Tanja Pipan, Nina Šajna, Slavko Polak, Tone Novak 2017 Karst Research Institute, Postojna

Mitigating The Conflict Between Pitfall-Trap Sampling And Conservation Of Terrestrial Subterranean Communities In Caves, Peter Kozel, Tanja Pipan, Nina Šajna, Slavko Polak, Tone Novak

International Journal of Speleology

Subterranean habitats are known for their rich endemic fauna and high vulnerability to disturbance. Many methods and techniques are used to sample the biodiversity of terrestrial invertebrate fauna in caves, among which pitfall trapping remains one of the most frequently used and effective ones. However, this method has turned out to be harmful to subterranean communities if applied inappropriately. Traditionally, pitfall traps have been placed in caves solely on the ground. Here we present an optimized technique of pitfall trapping to achieve a balance between sampling completeness and minimal disturbance of the fauna in the cave. Monthly we placed traps ...


Measuring Fertilization In Populations Of Sea Scallop (Placopecten Magellanicus): Developing And Testing Methods In The Laboratory And Field, Skylar Bayer 2017 University of Maine

Measuring Fertilization In Populations Of Sea Scallop (Placopecten Magellanicus): Developing And Testing Methods In The Laboratory And Field, Skylar Bayer

Electronic Theses and Dissertations

Most marine organisms are broadcast spawners, releasing their sperm and eggs into the water column. Methods of measuring in situ fertilization have proven successful with a few model species, which are reviewed in my introductory chapter. However, many commercially exploited species, such as the sea scallop Placopecten magellanicus, have been neglected. Sea scallop populations have greatly increased from fishing closures, but the mechanism behind this response is uncertain, particularly in regard to fertilization. In this dissertation I developed a methodology of measuring fertilization success and spawning events of P. magellanicus, tested it in laboratory and field settings, and developed a ...


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