Open Access. Powered by Scholars. Published by Universities.®

Research Methods in Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

464 Full-Text Articles 788 Authors 37,197 Downloads 58 Institutions

All Articles in Research Methods in Life Sciences

Faceted Search

464 full-text articles. Page 1 of 15.

Mapping Molecular Datasets Back To The Brain Regions They Are Extracted From: Remembering The Native Countries Of Hypothalamic Expatriates And Refugees, Arshad M. Khan, Alice H. Grant, Anais Martinez, Gully APC Burns, Brendan S. Thatcher, Vishwanath T. Anekonda, Benjamin W. Thompson, Zachary S. Roberts, Daniel H. Moralejo, James E. Blevins 2018 University of Texas at El Paso

Mapping Molecular Datasets Back To The Brain Regions They Are Extracted From: Remembering The Native Countries Of Hypothalamic Expatriates And Refugees, Arshad M. Khan, Alice H. Grant, Anais Martinez, Gully Apc Burns, Brendan S. Thatcher, Vishwanath T. Anekonda, Benjamin W. Thompson, Zachary S. Roberts, Daniel H. Moralejo, James E. Blevins

Arshad M. Khan, Ph.D.

This article, which includes novel unpublished data along with commentary and analysis,
focuses on approaches to link transcriptomic, proteomic, and peptidomic datasets mined from
brain tissue to the original locations within the brain that they are derived from using digital atlas
mapping techniques. We use, as an example, the transcriptomic, proteomic and peptidomic
analyses conducted in the mammalian hypothalamus. Following a brief historical overview, we
highlight studies that have mined biochemical and molecular information from the hypothalamus
and then lay out a strategy for how these data can be linked spatially to the mapped locations in a
canonical brain atlas ...


Digital Tools For Managing Different Steps Of The Systematic Review Process, Wendy Wu, Katherine Akers, Ella Hu, Alexandra Sarkozy, Patricia Vinson 2018 Wayne State University

Digital Tools For Managing Different Steps Of The Systematic Review Process, Wendy Wu, Katherine Akers, Ella Hu, Alexandra Sarkozy, Patricia Vinson

Library Scholarly Publications

Performing systematic reviews (SR) and meta-analyses is an arduous and time-consuming process that involves not only comprehensive literature searching but also record de-duplication, title/abstract and full-text screening, data extraction, quality assessment, statistical analysis, data visualization, report writing, and the creation of a bibliography. In the past several years, several digital tools and software have become available to facilitate different steps of the systematic review process. However, due to the growing number of tools, it can be difficult for systematic reviewers to make fully informed decisions about which tool(s) to use. Here, we (1) compile a comprehensive list of ...


The Effect Of Diet On Craniofacial Growth In Osteogenesis Imperfecta Mouse Model, Summer H. Ladd 2018 University of North Texas Health Science Center at Fort Worth

The Effect Of Diet On Craniofacial Growth In Osteogenesis Imperfecta Mouse Model, Summer H. Ladd

Theses and Dissertations

Osteogenesis imperfecta (OI, or “brittle bone disease”) is a rare disorder that is caused by genetic point mutations (COL1A1/COL1A2) that affect type 1 collagen. In OI type III (severe) patients, limb bones are more susceptible to skeletal fractures and the bones of the craniofacial region are underdeveloped. Some OI type III patients also suffer from dental malocclusions or fractures (dentinogenesis imperfecta, DI). The goals of this project are 1) to describe the facial phenotype in an OI mouse model, to see if this model can be used to test potential behavioral and pharmaceutical interventions; and 2) to determine if ...


Evolutionary Competition As Religion: A Religio-Biological Model Of The Maori And Vaisnava Sahajiya Traditions, Benjamin Highland 2018 College of William and Mary

Evolutionary Competition As Religion: A Religio-Biological Model Of The Maori And Vaisnava Sahajiya Traditions, Benjamin Highland

Undergraduate Honors Theses

Within the modern field of Religious Studies, there exists an epistemological divide between structuralist and post-structuralist thought. Structuralists seek to find underlying and universally applicable knowledge about religion, while post-structuralists argue that traditions must be studied within their own specific geographic, temporal, and cultural contexts. In an aim to reconcile these two disparate paradigms, I introduce the Religio-Biological Model and apply it to two distinct religious traditions: the Maori and the Vaisnava Sahajiya. Drawing from both religious and biological theory (specifically multilevel selection theory), this thesis seeks to create an interdisciplinary framework for the future study of religion that cohesively ...


Diversity, Structure And Sources Of Bacterial Communities In Earthworm Cocoons., Manuel Aira, Marcos Pérez-Losada, Jorge Domínguez 2018 George Washington University

Diversity, Structure And Sources Of Bacterial Communities In Earthworm Cocoons., Manuel Aira, Marcos Pérez-Losada, Jorge Domínguez

Computational Biology Institute

Animals start interactions with the bacteria that will constitute their microbiomes at embryonic stage. After mating, earthworms produce cocoons externally which will be colonized with bacteria from their parents and the environment. Due to the key role bacterial symbionts play on earthworm fitness, it is important to study bacterial colonization during cocoon formation. Here we describe the cocoon microbiome of the earthworms Eisenia andrei and E. fetida, which included 275 and 176 bacterial species, respectively. They were dominated by three vertically-transmitted symbionts, Microbacteriaceae, Verminephrobacter and Ca. Nephrothrix, which accounted for 88% and 66% of the sequences respectively. Verminephrobacter and Ca ...


Respiratory Disease, Behavior, And Survival Of Mountain Goat Kids, Julie A. Blanchong, Christopher A. Anderson, Nicholas Clark, Robert W. Klaver, Paul J. Plummer, Mike Cox, Caleb Mcadoo, Peregrine L. Wolff 2018 Iowa State University

Respiratory Disease, Behavior, And Survival Of Mountain Goat Kids, Julie A. Blanchong, Christopher A. Anderson, Nicholas Clark, Robert W. Klaver, Paul J. Plummer, Mike Cox, Caleb Mcadoo, Peregrine L. Wolff

Natural Resource Ecology and Management Publications

Bacterial pneumonia is a threat to bighorn sheep (Ovis canadensis) populations. Bighorn sheep in the East Humboldt Mountain Range (EHR), Nevada, USA, experienced a pneumonia epizootic in 2009–2010. Testing of mountain goats (Oreamnos americanus) that were captured or found dead on this range during and after the epizootic detected bacteria commonly associated with bighorn sheep pneumonia die‐offs. Additionally, in years subsequent to the bighorn sheep epizootic, the mountain goat population had low kid:adult ratios, a common outcome for bighorn sheep populations that have experienced a pneumonia epizootic. We hypothesized that pneumonia was present and negatively affecting mountain ...


The Subject Librarian Newsletter, Biology, Spring 2017, Sandy Avila 2018 University of Central Florida

The Subject Librarian Newsletter, Biology, Spring 2017, Sandy Avila

Sandy Avila

No abstract provided.


Glucocorticoids Modulate Gastrointestinal Microbiome In A Wild Bird., José C Noguera, Manuel Aira, Marcos Pérez-Losada, Jorge Domínguez, Alberto Velando 2018 George Washington University

Glucocorticoids Modulate Gastrointestinal Microbiome In A Wild Bird., José C Noguera, Manuel Aira, Marcos Pérez-Losada, Jorge Domínguez, Alberto Velando

Computational Biology Institute

It has recently been hypothesized that stress exposure (e.g. via glucocorticoid secretion) may dysregulate the bacterial gut microbiome, a crucial 'organ' in animal health. However, whether stress exposure (e.g. via glucocorticoid secretion) affects the bacterial gut microbiome of natural populations is unknown. We have experimentally altered the basal glucocorticoid level (corticosterone implants) in a wild avian species, the yellow-legged gull


Developing New Apparatus For In-Situ Vermicomposting And Varying Diet Parameters To Understand Optimal Conditions For Soil Fertilization., Nataliya Ryzhenko 2018 Lesley University

Developing New Apparatus For In-Situ Vermicomposting And Varying Diet Parameters To Understand Optimal Conditions For Soil Fertilization., Nataliya Ryzhenko

Lesley University Community of Scholars Day

Vermicomposting is the process of decomposing vegetable and other food waste using earthworms.

We investigate apparatus for in-situ vermicomposting and vary diet parameters to understand optimal conditions for soil fertilization.

As a pilot project, we created a small in-situ vermicomposting apparatus which can be used in a home or at a small farm. In this setting, we need to ensure that the setup does not produce repulsive odor, since it is meant to be kept indoors. Also, the setup should be easily maintained: the food waste easily added, and the bedding kept moist.

Based on results seen, in-situ vermicomposting farm ...


Studies Of Amino Acid Mutations In Drug Resistance Of The Smo Protein, Eunice Wintona 2018 University of Nebraska at Omaha

Studies Of Amino Acid Mutations In Drug Resistance Of The Smo Protein, Eunice Wintona

Student Research and Creative Activity Fair

Smoothened receptor (SMO) is a protein that in humans, is encoded by the SMO gene. A systemic mutation in its binding pocket helps predict the sensitivity of mutant proteins to different drugs. Known as a GPCR-like receptor, it is a component of the hedgehog signaling pathway; a pathway involved in body patterning and the regulation of adult stem cells. An uncontrolled or inappropriate activation of the Hedgehog pathway drives tumor progression in cancers and a number of birth defects. To achieve these goals, the molecular modeling software MOE was used to build small molecules and drug molecules like Vismodegib and ...


Addressing The Challenges Of Conducting Observational Studies In Sheep Abattoirs, Elyssa Payne, Melissa Starling, Paul McGreevy 2018 University of Sydney

Addressing The Challenges Of Conducting Observational Studies In Sheep Abattoirs, Elyssa Payne, Melissa Starling, Paul Mcgreevy

Paul McGreevy, Ph.D.

The competing needs of maintaining productivity within abattoirs, and maintaining high standards of animal welfare, provide fertile grounds for applied research in animal behavior. However, there are challenges involved in capturing useful behavioral data from the supply chain (from paddock to processing plant). The challenges identified in this report are based on a review of the scientific literature as well as field study observations. This article describes those challenges as they relate to collecting behavioral data on livestock-herding dogs, humans and livestock as they interact in abattoirs, and provides insights and recommendations for others embarking on animal studies in confined ...


Benchmark Evaluation Of True Single Molecular Sequencing To Determine Cystic Fibrosis Airway Microbiome Diversity, Andrea Hahn, Matthew L. Bendall, Keylie M. Gibson, Hollis Chaney, Iman Sami, Geovanny F. Perez, Anastassios C. Koumbourlis, Timothy A. McCaffrey, Robert J. Freishtat, Keith A Crandall 2018 George Washington University

Benchmark Evaluation Of True Single Molecular Sequencing To Determine Cystic Fibrosis Airway Microbiome Diversity, Andrea Hahn, Matthew L. Bendall, Keylie M. Gibson, Hollis Chaney, Iman Sami, Geovanny F. Perez, Anastassios C. Koumbourlis, Timothy A. Mccaffrey, Robert J. Freishtat, Keith A Crandall

Computational Biology Institute

Cystic fibrosis (CF) is an autosomal recessive disease associated with recurrent lung infections that can lead to morbidity and mortality. The impact of antibiotics for treatment of acute pulmonary exacerbations on the CF airway microbiome remains unclear with prior studies giving conflicting results and being limited by their use of 16S ribosomal RNA sequencing. Our primary objective was to validate the use of true single molecular sequencing (tSMS) and PathoScope in the analysis of the CF airway microbiome. Three control samples were created with differing amounts of Burkholderia cepacia, Pseudomonas aeruginosa, and Prevotella melaninogenica, three common bacteria found in cystic ...


Novel Serotonin 6 Receptor Antagonist And Its Effects On Autistic-Like Behaviors In The C58/J Mouse Strain, alexa bykowski 2018 University of Redlands

Novel Serotonin 6 Receptor Antagonist And Its Effects On Autistic-Like Behaviors In The C58/J Mouse Strain, Alexa Bykowski

Undergraduate Honors Theses

Autism spectrum disorder (ASD) is a neurodevelopmental disorder appearing in early childhood, characterized by three core symptoms: restricted, repetitive behaviors (RRBs), deficits in social interaction, and a shortfall in communication. The mechanism underlying the manifestation of ASD is unknown but is likely caused by interactions between genes and the environment. The serotonin (5-HT) system has been implicated as a potential mechanism explaining autistic behaviors, as elective serotonin reuptake inhibitors (SSRIs) have historically been used off-label to help treat symptoms of autism but can have many adverse side effects. By using a novel therapeutic specifically targeting the 5-HT6 receptor, there is ...


Disarming “Nature” As A Weapon: A Queer Ecosemiotic Reimagining Of Futurity And Environmental Ethics Through Memoir, Sam Lauer 2018 Bucknell University

Disarming “Nature” As A Weapon: A Queer Ecosemiotic Reimagining Of Futurity And Environmental Ethics Through Memoir, Sam Lauer

Master’s Theses

In this thesis, I posit that the need for an active, conscious, and radical queering of ecocriticism as a literary and cultural theory has arisen in light of the postmodern problematization of “nature” and the “natural,” along with the queerness of society, culture, and science. The way we understand “nature” (in life and in texts), whether of physical environments, inherent selfhood, or normalcy, begs to be appropriately informed by discourses and realities of queerness in order for both social and environmental healing to take place. I have analyzed three works of queer creative nonfiction—memoirs—to illuminate the ways in ...


Prior Voluntary Wheel Running Prevents Memory Deficits And Enhanced Neuropathic Pain Presenting After Morphine Dosing Ceases, Nathan Plattner 2018 University of Colorado, Boulder

Prior Voluntary Wheel Running Prevents Memory Deficits And Enhanced Neuropathic Pain Presenting After Morphine Dosing Ceases, Nathan Plattner

Undergraduate Honors Theses

Clinical research studies show that chronic pain is a prevalent, difficult affliction to manage. The most common management for chronic pain is opioids, which research is showing to paradoxically induce persistent, worsened pain states as well as potentially having negative effects on memory. The necessity for understanding chronic pain and drug management options is apparent, and research is aiming to do just that. Exercise is one potential preventative measure or post hoc treatment possibility for chronic pain, as abundant research has shown. Knowledge of the interactions between exercise, morphine dosing, and peripheral nerve injury is nonexistent. As such, we desired ...


Possible Breakdown Of Dopamine Receptor Synergism In A Mouse Model Of Huntington's Disease, Samantha F. Kennedy 2017 University of New Orleans

Possible Breakdown Of Dopamine Receptor Synergism In A Mouse Model Of Huntington's Disease, Samantha F. Kennedy

University of New Orleans Theses and Dissertations

The model of basal ganglia function proposed by Albin, Young and Penney (1989) describes two anatomically independent motor pathways, the direct and indirect. However, under normal conditions striatal dopamine (DA) is required for the expression of motor behavior, and DAergic control of the two pathways (via D1 and D2 receptors, respectively) is dependent on co-activation. We tested for a possible breakdown of D1/D2 synergism using transgenic R6/1 mice bearing the human huntingtin allele (Htt). Motor stereotypy, observed prior to the onset of HD-related symptoms, was rated on a 5-point scale following activation of: A) D1 receptors alone, B ...


Marina365 Agen Casino Online, Judi Casino, Casino Online Terbaik.Pdf, Sulis Setyo Sulis Setyo 2017 SKy

Marina365 Agen Casino Online, Judi Casino, Casino Online Terbaik.Pdf, Sulis Setyo Sulis Setyo

Sulis Setyo

MARINA365 AGEN CASINO ONLINE, JUDI CASINO, CASINO ONLINE TERBAIK | Jika engkau sedang cari info berkenaan dengan Info Berkaitan dengan MARINA365 AGEN JUDI CASINO ONLINE TERBAIK kamu tersesat di website yang tepat. Saya senang bagi-bagi artikel, Maka dari itu Saya akan berikan kamu info yang bagus mengenai Info Berkaitan dengan MARINA365 AGEN JUDI CASINO ONLINE TERBAIK. 
Informasi Partner Kontes seo INCARQQ.net Agen Sakong Online Terpercaya


Effects Of Trophic Relationships On Oyster Reef Restoration Success In The Mississippi Sound, Virginia Robin Fleer 2017 The University of Southern Mississippi

Effects Of Trophic Relationships On Oyster Reef Restoration Success In The Mississippi Sound, Virginia Robin Fleer

Dissertations

Natural and anthropogenic changes resulting from altered hydrology, hurricanes, variable precipitation, and the BP oil spill have all taken their toll on oyster reefs in Mississippi. In response, oyster reef restoration efforts are currently underway within the Northern Gulf of Mexico. In order to understand why these efforts succeed or fail, it is crucial to consider predator-prey relationships within the context of the trophic dynamics of oyster reefs. Thus, for this dissertation study I integrated a multidisciplinary approach to understanding key trophic interactions affecting oyster recruitment, growth and survival, comprising field sampling, manipulative lab experiments, and individual-based modeling. Spat settlement ...


Signature Whistle Production During A Bottlenose Dolphin Group Integration, Megan S. Broadway 2017 University of Southern Mississippi

Signature Whistle Production During A Bottlenose Dolphin Group Integration, Megan S. Broadway

Dissertations

Bottlenose dolphins are an important species of interest because they possess a variety of abilities that are relatively rare in the animal kingdom, one being complex acoustic communication. Signature whistles - distinctive calls that are unique for each individual – are one of the most studied call types, but we know little about how these calls are used in various contexts, such as during an introduction. Looking at the socio-behavioral context in which signature whistles are used is likely the best way of learning how these whistles are used in a particular context (Caldwell, Caldwell, & Tyack, 1990). For this project, the behavior ...


Structural Insights Into The Potency Of Sk Channel Positive Modulators, Young-Woo Nam, Razan Orfali, Tingting Liu, Kunqian Yu, Meng Cui, Heike Wulff, Miao Zhang 2017 Chapman University

Structural Insights Into The Potency Of Sk Channel Positive Modulators, Young-Woo Nam, Razan Orfali, Tingting Liu, Kunqian Yu, Meng Cui, Heike Wulff, Miao Zhang

Pharmacy Faculty Articles and Research

Small-conductance Ca2+-activated K+ (SK) channels play essential roles in the regulation of cellular excitability and have been implicated in neurological and cardiovascular diseases through both animal model studies and human genetic association studies. Over the past two decades, positive modulators of SK channels such as NS309 and 1-EBIO have been developed. Our previous structural studies have identified the binding pocket of 1-EBIO and NS309 that is located at the interface between the channel and calmodulin. In this study, we took advantage of four compounds with potencies varying over three orders of magnitude, including 1-EBIO, NS309, SKS-11 (6-bromo-5-methyl-1H-indole-2 ...


Digital Commons powered by bepress