Open Access. Powered by Scholars. Published by Universities.®

Research Methods in Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

622 Full-Text Articles 1,002 Authors 37,197 Downloads 61 Institutions

All Articles in Research Methods in Life Sciences

Faceted Search

622 full-text articles. Page 1 of 17.

Development Of A Pd-L1 Pet Imaging Biomarker, Caleb Jack Bridgwater 2018 University of Louisville

Development Of A Pd-L1 Pet Imaging Biomarker, Caleb Jack Bridgwater

Posters-at-the-Capitol

Immunotherapy strategies are very promising treatments for cancer patients. Specifically, Immune checkpoint inhibitor therapy focusing on the PD-1/PD-L1 pathway shows long-lasting positive results in many cancer patients. Unfortunately, not all the patients can benefit from this highly effective treatment. Hence, there is a great need for predictive biomarkers. Immunohistochemical (IHC) staining has been used as a way of predicting patient response, yet shows many problems. For example, IHC utilizes an invasive biopsy and sample fixing, which creates an incomplete and delayed picture of the patient’s biochemistry and the tumor microenvironment, consequently ignoring metastases.

The purpose of this study ...


A Cohort-Based Program To Help Students Prepare A Conference Research Presentation, Alanna Lecher 2018 Lynn University

A Cohort-Based Program To Help Students Prepare A Conference Research Presentation, Alanna Lecher

Florida Statewide Symposium: Best Practices in Undergraduate Research

Students move through many first time experiences when navigating their undergraduate and graduate education. Such experiences include the first time students submit an article to a peer-reviewed scientific journal, attend a conference, and conduct fieldwork. The cohort model has been shown to be effective in increasing success in undergraduate education, and it can be adapted to helping students succeed in these novel experiences as well. This presentation will explore one program where the cohort model was implemented to aid undergraduate students preparing their first conference presentation on a scientific research project. Program structure and implementation will be described.


Research And Publication: Are We Moving In The Right Direction, A Khan, Rehana Rehman, Saara Ahmad 2018 Aga Khan University

Research And Publication: Are We Moving In The Right Direction, A Khan, Rehana Rehman, Saara Ahmad

Department of Biological & Biomedical Sciences

No abstract provided.


Measurement And Protection Of Lung Health In Poultry Farmers Of Southwestern Ontario, Rose-Marie Doyon Dolinar 2018 The University of Western Ontario

Measurement And Protection Of Lung Health In Poultry Farmers Of Southwestern Ontario, Rose-Marie Doyon Dolinar

Electronic Thesis and Dissertation Repository

Chronic obstructive lung disease (COPD), develops gradually, and tends to be noticed by farmers only after there has been a significant loss of lung function. People with symptoms of COPD often present for health care in later stages of disease, because they accept cough or breathlessness as “normal”.

Measurement of lung health for those at risk of developing COPD provides an opportunity for early detection of lung disease, and deter the progression toward irreversible damage to the lungs. COPD is, however, under-diagnosed.

The importance of bringing primary care providers and farmers together, and acknowledging the high-risk nature of occupational exposures ...


Targeting Neuropeptides To Bone Fractures For Accelerated Healing, Nicholas A. Young, Jeffery J. Nielsen, Philip S. Low 2018 Purdue University

Targeting Neuropeptides To Bone Fractures For Accelerated Healing, Nicholas A. Young, Jeffery J. Nielsen, Philip S. Low

The Summer Undergraduate Research Fellowship (SURF) Symposium

In patients over the age of 65 especially, bone fractures represent a significant disease burden. Non-invasive drug therapies are not available for bone fractures which represents a problem for this population. Vasoactive intestinal peptide (VIP) and Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP), two neuromodulator peptides in the glucagon superfamily, have demonstrated positive regulation of osteoblast proliferation and activity. Using acidic oligopeptides, we have developed ligands that target to and accumulate at fracture sites. These targeting ligands can be synthesized in sequence with bone anabolic peptides to minimize off target effects and increase potency at the fracture site to create safer and ...


Investigation Of Zebrafish Larvae Behavior As Precursor For Suborbital Flights: Feasibility Study, Pedro Llanos, Kristina Andrijauskaite, Mark Rubinstein, Sherine S.L. Chan 2018 Embry-Riddle Aeronautical University - Daytona Beach

Investigation Of Zebrafish Larvae Behavior As Precursor For Suborbital Flights: Feasibility Study, Pedro Llanos, Kristina Andrijauskaite, Mark Rubinstein, Sherine S.L. Chan

Pedro J. Llanos (www.AstronauticsLlanos.com)


Suborbital spaceflights, carrying scientific payloads, allow scientists not only to test the feasibility of their payloads, but they also provide the basis for refining scientific hypotheses to be later tested on the International Space Station (ISS). Therefore, it is essential to establish robust pre-flight procedures in order to take advantage of this unique research platform to facilitate payload delivery. In the present study, we assessed zebrafish larvae behavior as a precursor for the future suborbital spaceflight involving research on the musculoskeletal system. Zebrafish larvae were exposed to the same physiological stressors they would encounter during suborbital spaceflight: alterations in light ...


Identifying The 5’End Of The Camta1 Genes In Zebrafish, Morgan Fronk 2018 Brigham Young University

Identifying The 5’End Of The Camta1 Genes In Zebrafish, Morgan Fronk

Undergraduate Honors Theses

Congenital heart diseases (CHDs) are a significant cause of infant death and are frequently caused by mutations in transcription factors. Camta1 (calmodulin binding transcription activator 1) is a transcription factor that has been proposed as a modulator in embryonic heart development and a possible cause of CHDs. The only other known member of its family in vertebrates is involved in activating a hypertrophy gene program in adult heart failure. Unlike camta2, camta1 is expressed in the embryonic heart during heart looping. However, few studies have been done on camta1. In zebrafish, there are two camta1 ohnologs (homologs created through a ...


Investigations Of Biotremors In The Veiled Chameleon (Chamaeleo Calyptratus), Kathryn C. Laslie 2018 Western Kentucky University

Investigations Of Biotremors In The Veiled Chameleon (Chamaeleo Calyptratus), Kathryn C. Laslie

Masters Theses & Specialist Projects

While substrate-borne vibrations are utilized by different reptile species, true conspecific communication via biotremors has not yet been demonstrated in reptiles. This study follows a preliminary report that the veiled chameleon (Chamaeleo calyptratus) could produce biotremors in communicative contexts. I tested chameleon behavioral sensitivity to vibrations by placing them on a dowel attached to a shaker emitting vibrations of 25, 50, 150, 300, and 600 Hz and then measured their changes in velocity before and after the stimulus. I then paired chameleons in various social contexts [anthropogenic disturbance (human disruption of animal); dominance (malemale; female-female C. calyptratus); courtship (male-female C ...


Mapping Molecular Datasets Back To The Brain Regions They Are Extracted From: Remembering The Native Countries Of Hypothalamic Expatriates And Refugees, Arshad M. Khan, Alice H. Grant, Anais Martinez, Gully APC Burns, Brendan S. Thatcher, Vishwanath T. Anekonda, Benjamin W. Thompson, Zachary S. Roberts, Daniel H. Moralejo, James E. Blevins 2018 University of Texas at El Paso

Mapping Molecular Datasets Back To The Brain Regions They Are Extracted From: Remembering The Native Countries Of Hypothalamic Expatriates And Refugees, Arshad M. Khan, Alice H. Grant, Anais Martinez, Gully Apc Burns, Brendan S. Thatcher, Vishwanath T. Anekonda, Benjamin W. Thompson, Zachary S. Roberts, Daniel H. Moralejo, James E. Blevins

Arshad M. Khan, Ph.D.

This article, which includes novel unpublished data along with commentary and analysis,
focuses on approaches to link transcriptomic, proteomic, and peptidomic datasets mined from
brain tissue to the original locations within the brain that they are derived from using digital atlas
mapping techniques. We use, as an example, the transcriptomic, proteomic and peptidomic
analyses conducted in the mammalian hypothalamus. Following a brief historical overview, we
highlight studies that have mined biochemical and molecular information from the hypothalamus
and then lay out a strategy for how these data can be linked spatially to the mapped locations in a
canonical brain atlas ...


Bird Abundance At Bird Feeders In Response To Temperature, Wind Speed And Precipitation During The Winter Season, Siddhant Kahal 2018 California Polytechnic State University, San Luis Obispo

Bird Abundance At Bird Feeders In Response To Temperature, Wind Speed And Precipitation During The Winter Season, Siddhant Kahal

Master's Theses and Project Reports

The goal of this project is to explore how 23 different bird species respond to 3 climatic attributes. These attributes are lower than average temperatures, wind speed and precipitation level. Information about the bird species and all of the data associated with them is provided by Project FeederWatch (PFW). This is a citizen based survey study that provides key information about bird species abundance through the use of backyard and community feeders. The study volunteers from across the United States and Canada monitor these bird feeders and note important information about the species such as the number of individuals seen ...


Recta: Regulon Identification Based On Comparative Genomics And Transcriptomics Analysis, Xin Chen, Anjun Ma, Adam McDermaid, Hanyuan Zhang, Chao Liu, Huansheng Cao, Qin Ma 2018 Tianjin University,

Recta: Regulon Identification Based On Comparative Genomics And Transcriptomics Analysis, Xin Chen, Anjun Ma, Adam Mcdermaid, Hanyuan Zhang, Chao Liu, Huansheng Cao, Qin Ma

CSE Journal Articles

Regulons, which serve as co-regulated gene groups contributing to the transcriptional regulation of microbial genomes, have the potential to aid in understanding of underlying regulatory mechanisms. In this study, we designed a novel computational pipeline, regulon identification based on comparative genomics and transcriptomics analysis (RECTA), for regulon prediction related to the gene regulatory network under certain conditions. To demonstrate the effectiveness of this tool, we implemented RECTA on Lactococcus lactis MG1363 data to elucidate acid-response regulons. A total of 51 regulons were identified, 14 of which have computational-verified significance. Among these 14 regulons, five of them were computationally predicted to ...


The Wide Utility Of Rabbits As Models Of Human Diseases, Pedro J. Esteves, Yuxing Chen, Shuying Liu, Shan Lu, Michael Vaine, Shixia Wang, Rose Mage 2018 Universidade do Porto, Portugal

The Wide Utility Of Rabbits As Models Of Human Diseases, Pedro J. Esteves, Yuxing Chen, Shuying Liu, Shan Lu, Michael Vaine, Shixia Wang, Rose Mage

Open Access Articles

Studies using the European rabbit Oryctolagus cuniculus contributed to elucidating numerous fundamental aspects of antibody structure and diversification mechanisms and continue to be valuable for the development and testing of therapeutic humanized polyclonal and monoclonal antibodies. Additionally, during the last two decades, the use of the European rabbit as an animal model has been increasingly extended to many human diseases. This review documents the continuing wide utility of the rabbit as a reliable disease model for development of therapeutics and vaccines and studies of the cellular and molecular mechanisms underlying many human diseases. Examples include syphilis, tuberculosis, HIV-AIDS, acute hepatic ...


Digital Tools For Managing Different Steps Of The Systematic Review Process, Wendy Wu, Katherine Akers, Ella Hu, Alexandra Sarkozy, Patricia Vinson 2018 Wayne State University

Digital Tools For Managing Different Steps Of The Systematic Review Process, Wendy Wu, Katherine Akers, Ella Hu, Alexandra Sarkozy, Patricia Vinson

Library Scholarly Publications

Performing systematic reviews (SR) and meta-analyses is an arduous and time-consuming process that involves not only comprehensive literature searching but also record de-duplication, title/abstract and full-text screening, data extraction, quality assessment, statistical analysis, data visualization, report writing, and the creation of a bibliography. In the past several years, several digital tools and software have become available to facilitate different steps of the systematic review process. However, due to the growing number of tools, it can be difficult for systematic reviewers to make fully informed decisions about which tool(s) to use. Here, we (1) compile a comprehensive list of ...


Juvenile Amphibian Response To Oak And Maple Leaf Litter, Benjamin Breslau 2018 University of Connecticut - Storrs

Juvenile Amphibian Response To Oak And Maple Leaf Litter, Benjamin Breslau

Honors Scholar Theses

The composition of tree species within New England forests has changed significantly in recent decades, with an increase in maple (Acer spp.) abundance and a decrease in oak (Quercus spp.) abundance. Changing forest structure results in changing leaf litter composition of the forest floor, which influences the ground-dwelling amphibians that live in the litter. To better understand how changes to forest composition alters amphibian habitat quality, we recorded the growth and survival of 27 juvenile wood frogs (Lithobates sylvaticus or Rana sylvatica) and 27 juvenile American toads (Anaxyrus americanus) in response to leaf litter type. Between early August and late ...


The Effect Of Diet On Craniofacial Growth In Osteogenesis Imperfecta Mouse Model, Summer H. Ladd 2018 University of North Texas Health Science Center at Fort Worth

The Effect Of Diet On Craniofacial Growth In Osteogenesis Imperfecta Mouse Model, Summer H. Ladd

Theses and Dissertations

Osteogenesis imperfecta (OI, or “brittle bone disease”) is a rare disorder that is caused by genetic point mutations (COL1A1/COL1A2) that affect type 1 collagen. In OI type III (severe) patients, limb bones are more susceptible to skeletal fractures and the bones of the craniofacial region are underdeveloped. Some OI type III patients also suffer from dental malocclusions or fractures (dentinogenesis imperfecta, DI). The goals of this project are 1) to describe the facial phenotype in an OI mouse model, to see if this model can be used to test potential behavioral and pharmaceutical interventions; and 2) to determine if ...


Pyruvate Kinase Isoform M2 Influences Autophagy And Related Processes In Hepatocellular Carcinoma Cells, Matthew Lin 2018 University of Connecticut

Pyruvate Kinase Isoform M2 Influences Autophagy And Related Processes In Hepatocellular Carcinoma Cells, Matthew Lin

University Scholar Projects

Hepatocellular carcinoma (HCC) is the most common form of liver cancer that affects ~14 million people in the world. Like all cancers, HCC is a disease that arises from unstinted cellular growth initiated by genetic alterations, metabolic changes, and dysregulation in key cellular pathways. Of interest is the relationship between metabolism and cell proliferation/degradation for therapeutic targeting. Pyruvate kinase M2 is a dimeric, glycolytically inactive isoform of the final enzyme involved in glycolysis, that is often upregulated in cancerous tissue. It is thought that the enzymatic function of PKM2 outside of glycolysis contributes to the biosynthesis of anabolic intermediates ...


Evolutionary Competition As Religion: A Religio-Biological Model Of The Maori And Vaisnava Sahajiya Traditions, Benjamin Highland 2018 College of William and Mary

Evolutionary Competition As Religion: A Religio-Biological Model Of The Maori And Vaisnava Sahajiya Traditions, Benjamin Highland

Undergraduate Honors Theses

Within the modern field of Religious Studies, there exists an epistemological divide between structuralist and post-structuralist thought. Structuralists seek to find underlying and universally applicable knowledge about religion, while post-structuralists argue that traditions must be studied within their own specific geographic, temporal, and cultural contexts. In an aim to reconcile these two disparate paradigms, I introduce the Religio-Biological Model and apply it to two distinct religious traditions: the Maori and the Vaisnava Sahajiya. Drawing from both religious and biological theory (specifically multilevel selection theory), this thesis seeks to create an interdisciplinary framework for the future study of religion that cohesively ...


Diversity, Structure And Sources Of Bacterial Communities In Earthworm Cocoons., Manuel Aira, Marcos Pérez-Losada, Jorge Domínguez 2018 George Washington University

Diversity, Structure And Sources Of Bacterial Communities In Earthworm Cocoons., Manuel Aira, Marcos Pérez-Losada, Jorge Domínguez

Computational Biology Institute

Animals start interactions with the bacteria that will constitute their microbiomes at embryonic stage. After mating, earthworms produce cocoons externally which will be colonized with bacteria from their parents and the environment. Due to the key role bacterial symbionts play on earthworm fitness, it is important to study bacterial colonization during cocoon formation. Here we describe the cocoon microbiome of the earthworms Eisenia andrei and E. fetida, which included 275 and 176 bacterial species, respectively. They were dominated by three vertically-transmitted symbionts, Microbacteriaceae, Verminephrobacter and Ca. Nephrothrix, which accounted for 88% and 66% of the sequences respectively. Verminephrobacter and Ca ...


Respiratory Disease, Behavior, And Survival Of Mountain Goat Kids, Julie A. Blanchong, Christopher A. Anderson, Nicholas Clark, Robert W. Klaver, Paul J. Plummer, Mike Cox, Caleb Mcadoo, Peregrine L. Wolff 2018 Iowa State University

Respiratory Disease, Behavior, And Survival Of Mountain Goat Kids, Julie A. Blanchong, Christopher A. Anderson, Nicholas Clark, Robert W. Klaver, Paul J. Plummer, Mike Cox, Caleb Mcadoo, Peregrine L. Wolff

Natural Resource Ecology and Management Publications

Bacterial pneumonia is a threat to bighorn sheep (Ovis canadensis) populations. Bighorn sheep in the East Humboldt Mountain Range (EHR), Nevada, USA, experienced a pneumonia epizootic in 2009–2010. Testing of mountain goats (Oreamnos americanus) that were captured or found dead on this range during and after the epizootic detected bacteria commonly associated with bighorn sheep pneumonia die‐offs. Additionally, in years subsequent to the bighorn sheep epizootic, the mountain goat population had low kid:adult ratios, a common outcome for bighorn sheep populations that have experienced a pneumonia epizootic. We hypothesized that pneumonia was present and negatively affecting mountain ...


The Subject Librarian Newsletter, Biology, Spring 2017, Sandy Avila 2018 University of Central Florida

The Subject Librarian Newsletter, Biology, Spring 2017, Sandy Avila

Sandy Avila

No abstract provided.


Digital Commons powered by bepress