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Mcnamara 20171227w Draft - Smfish And Qif Plus Optical Clearing Li Germain Gerner 2017 Pnas - Gi Division Short Talk.Pdf, George McNamara 2017 M.D. Anderson Cancer Center

Mcnamara 20171227w Draft - Smfish And Qif Plus Optical Clearing Li Germain Gerner 2017 Pnas - Gi Division Short Talk.Pdf, George Mcnamara

George McNamara

Unpublished (except here at bepress) presentation on single molecule RNA fluorescence in situ hybridization (smFISH) and quantitative immunofluorescence microscopy (qIF) plus optical clearing of tissues. Features "Ce3D" mpaper by Li Germain Gerner 2017 PNAS.


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Effects Of Trophic Relationships On Oyster Reef Restoration Success In The Mississippi Sound, Virginia Robin Fleer 2017 The University of Southern Mississippi

Effects Of Trophic Relationships On Oyster Reef Restoration Success In The Mississippi Sound, Virginia Robin Fleer

Dissertations

Natural and anthropogenic changes resulting from altered hydrology, hurricanes, variable precipitation, and the BP oil spill have all taken their toll on oyster reefs in Mississippi. In response, oyster reef restoration efforts are currently underway within the Northern Gulf of Mexico. In order to understand why these efforts succeed or fail, it is crucial to consider predator-prey relationships within the context of the trophic dynamics of oyster reefs. Thus, for this dissertation study I integrated a multidisciplinary approach to understanding key trophic interactions affecting oyster recruitment, growth and survival, comprising field sampling, manipulative lab experiments, and individual-based modeling. Spat settlement ...


Signature Whistle Production During A Bottlenose Dolphin Group Integration, Megan S. Broadway 2017 University of Southern Mississippi

Signature Whistle Production During A Bottlenose Dolphin Group Integration, Megan S. Broadway

Dissertations

Bottlenose dolphins are an important species of interest because they possess a variety of abilities that are relatively rare in the animal kingdom, one being complex acoustic communication. Signature whistles - distinctive calls that are unique for each individual – are one of the most studied call types, but we know little about how these calls are used in various contexts, such as during an introduction. Looking at the socio-behavioral context in which signature whistles are used is likely the best way of learning how these whistles are used in a particular context (Caldwell, Caldwell, & Tyack, 1990). For this project, the behavior ...


Structural Insights Into The Potency Of Sk Channel Positive Modulators, Young-Woo Nam, Razan Orfali, Tingting Liu, Kunqian Yu, Meng Cui, Heike Wulff, Miao Zhang 2017 Chapman University

Structural Insights Into The Potency Of Sk Channel Positive Modulators, Young-Woo Nam, Razan Orfali, Tingting Liu, Kunqian Yu, Meng Cui, Heike Wulff, Miao Zhang

Pharmacy Faculty Articles and Research

Small-conductance Ca2+-activated K+ (SK) channels play essential roles in the regulation of cellular excitability and have been implicated in neurological and cardiovascular diseases through both animal model studies and human genetic association studies. Over the past two decades, positive modulators of SK channels such as NS309 and 1-EBIO have been developed. Our previous structural studies have identified the binding pocket of 1-EBIO and NS309 that is located at the interface between the channel and calmodulin. In this study, we took advantage of four compounds with potencies varying over three orders of magnitude, including 1-EBIO, NS309, SKS-11 (6-bromo-5-methyl-1H-indole-2 ...


Spinal Cord Injury Disrupts Circadian Rhythms In Rats, Emily M. Bateman 2017 University of Colorado Boulder

Spinal Cord Injury Disrupts Circadian Rhythms In Rats, Emily M. Bateman

Psychology and Neuroscience Undergraduate Contributions

Traumatic spinal cord injury affects 282,000 people in the United States alone. Individuals often suffer from partial paralysis and chronic pain along with physiological impairments, such as abnormal thermoregulation, decreased motor function, and poor sleep quality. Current clinical therapies focus on symptomatic treatments. Despite extensive research, all of the physiologic effects and the molecular mechanism underlying spinal cord injury, and how they affect each other, remains elusive. Here, we tested whether spinal cord injury disrupted circadian rhythms, which could prolong the recovery process. We used implanted transmitters in male and female rats to measure a marker, body temperature, and ...


Theta-Burst Microstimulation In The Human Entorhinal Area Improves Memory Specificity, Ali S. Titiz, Michael R. H. Hill, Emily A. Mankin, Zahra M. Aghajan, Dawn Eliashiv, Natalia Tchemodanov, Uri Maoz, John Stern, Michelle E. Tran, Peter Schuette, Eric Behnke, Nanthia A. Suthana, Itzhak Fried 2017 University of California, Los Angeles

Theta-Burst Microstimulation In The Human Entorhinal Area Improves Memory Specificity, Ali S. Titiz, Michael R. H. Hill, Emily A. Mankin, Zahra M. Aghajan, Dawn Eliashiv, Natalia Tchemodanov, Uri Maoz, John Stern, Michelle E. Tran, Peter Schuette, Eric Behnke, Nanthia A. Suthana, Itzhak Fried

Psychology Faculty Articles and Research

The hippocampus is critical for episodic memory, and synaptic changes induced by long-term potentiation (LTP) are thought to underlie memory formation. In rodents, hippocampal LTP may be induced through electrical stimulation of the perforant path. To test whether similar techniques could improve episodic memory in humans, we implemented a microstimulation technique that allowed delivery of low-current electrical stimulation via 100 μm-diameter microelectrodes. As thirteen neurosurgical patients performed a person recognition task, microstimulation was applied in a theta-burst pattern, shown to optimally induce LTP. Microstimulation in the right entorhinal area during learning significantly improved subsequent memory specificity for novel portraits; participants ...


Alcohol Consumption Impairs The Ependymal Cilia Motility In The Brain Ventricles, Alzahra J. Al Omran, Hannah C. Saternos, Yusuf S. Althobaiti, Alexander Wisner, Youssef Sari, Surya M. Nauli, Wissam A. AbouAlaiwi 2017 University of Toledo

Alcohol Consumption Impairs The Ependymal Cilia Motility In The Brain Ventricles, Alzahra J. Al Omran, Hannah C. Saternos, Yusuf S. Althobaiti, Alexander Wisner, Youssef Sari, Surya M. Nauli, Wissam A. Aboualaiwi

Pharmacy Faculty Articles and Research

Ependymal cilia protrude into the central canal of the brain ventricles and spinal cord to circulate the cerebral spinal fluid (CSF). Ependymal cilia dysfunction can hinder the movement of CSF leading to an abnormal accumulation of CSF within the brain known as hydrocephalus. Although the etiology of hydrocephalus was studied before, the effects of ethanol ingestion on ependymal cilia function have not been investigated in vivo. Here, we report three distinct types of ependymal cilia, type-I, type-II and type-III classified based upon their beating frequency, their beating angle, and their distinct localization within the mouse brain-lateral ventricle. Our studies show ...


Phylogenetic Evidence From Freshwater Crayfishes That Cave Adaptation Is Not An Evolutionary Dead-End., David B Stern, Jesse Breinholt, Carlos Pedraza-Lara, Marilú López-Mejía, Christopher L Owen, Heather Bracken-Grissom, James W Fetzner, Keith A Crandall 2017 George Washington University

Phylogenetic Evidence From Freshwater Crayfishes That Cave Adaptation Is Not An Evolutionary Dead-End., David B Stern, Jesse Breinholt, Carlos Pedraza-Lara, Marilú López-Mejía, Christopher L Owen, Heather Bracken-Grissom, James W Fetzner, Keith A Crandall

Computational Biology Institute

Caves are perceived as isolated, extreme habitats with a uniquely specialized biota, which long ago led to the idea that caves are "evolutionary dead-ends." This implies that cave-adapted taxa may be doomed for extinction before they can diversify or transition to a more stable state. However, this hypothesis has not been explicitly tested in a phylogenetic framework with multiple independently evolved cave-dwelling groups. Here, we use the freshwater crayfish, a group with dozens of cave-dwelling species in multiple lineages, as a system to test this hypothesis. We consider historical patterns of lineage diversification and habitat transition as well as current ...


Evaluation Of Surgical Methods For Treatment Of Cubital Tunnel Syndrome - Statistical Perspectives, Ahmed Mohammed Morsy 2017 Assiut University, South Egypt Cancer Institute, Pediatric Oncology Department

Evaluation Of Surgical Methods For Treatment Of Cubital Tunnel Syndrome - Statistical Perspectives, Ahmed Mohammed Morsy

Dr/ Ahmed Mohammed Morsy

This commentary addresses strengths and weaknesses of the clinical trial study protocol designed by Liu et al., in particular with regard of the methodology, taking into consideration the commentary article "Assessing surgical methods for treatment of cubital tunnel syndrome - which is the best?" Our commentary emphasizes the importance of adjustment for known prognostic covariates, such as duration of symptoms and advanced age that have been negatively correlated with outcomes in previous studies. Subgroup analysis for the treatment groups of interest, namely moderate and severe cubital tunnel syndrome, which have previously shown conflicting differences in efficacy of surgical options is highly ...


Amelioration Of Prenatal Alcohol Effects By Environmental Enrichment In A Mouse Model Of Fasd, Aniruddho Chokroborty-Hoque 2017 The University of Western Ontario

Amelioration Of Prenatal Alcohol Effects By Environmental Enrichment In A Mouse Model Of Fasd, Aniruddho Chokroborty-Hoque

Electronic Thesis and Dissertation Repository

Maternal alcohol consumption during pregnancy results in a spectrum of behavioural and cognitive deficits collectively known as Fetal Alcohol Spectrum Disorders (FASD). Currently, little is know about if and how the external environment may modulate these deficits. I have used C57BL/6 mice to study this interaction between prenatal alcohol exposure and the postnatal environment. Alcohol exposure during synaptogenesis produces high levels of anxiety-like traits and decreased memory performance. Alcohol-exposed mice (and matched unexposed controls) were put in 'environmentally-enriched' conditions of voluntary exercise, physical activities and cognitive stimulation to ascertain the effects of a positive postnatal environment. The results show ...


T-Time: A Data Repository Of T Cell And Calcium Release-Activated Calcium Channel Activation Imagery, Cody Arbuckle, Milton L. Greenberg, Adrienne Bergh, Rene German, Nick Sirago, Erik J. Linstead 2017 Chapman University

T-Time: A Data Repository Of T Cell And Calcium Release-Activated Calcium Channel Activation Imagery, Cody Arbuckle, Milton L. Greenberg, Adrienne Bergh, Rene German, Nick Sirago, Erik J. Linstead

Mathematics, Physics, and Computer Science Faculty Articles and Research

BACKGROUND: A fundamental understanding of live-cell dynamics is necessary in order to advance scientific techniques and personalized medicine. For this understanding to be possible, image processing techniques, probes, tracking algorithms and many other methodologies must be improved. Currently there are no large open-source datasets containing live-cell imaging to act as a standard for the community. As a result, researchers cannot evaluate their methodologies on an independent benchmark or leverage such a dataset to formulate scientific questions.

FINDINGS: Here we present T-Time, the largest free and publicly available data set of T cell phase contrast imagery designed with the intention of ...


A Review Of The Institute Of Medicine’S Analysis Of Using Chimpanzees In Biomedical Research, Robert C. Jones, Ray Greek 2017 California State University, Chico

A Review Of The Institute Of Medicine’S Analysis Of Using Chimpanzees In Biomedical Research, Robert C. Jones, Ray Greek

Robert C. Jones, Ph.D.

We argue that the recommendations made by the Institute of Medicine’s 2011 report, Chimpanzees in Biomedical and Behavioral Research: Assessing the Necessity, are methodologically and ethically confused. We argue that a proper understanding of evolution and complexity theory in terms of the science and ethics of using chimpanzees in biomedical research would have had led the committee to recommend not merely limiting but eliminating the use of chimpanzees in biomedical research. Specifically, we argue that a proper understanding of the difference between the gross level of examination of species and examinations on finer levels can shed light on important ...


Transcriptomic Differentiation Underlying Marine‐To‐Freshwater Transitions In The South American Silversides Odontesthes Argentinensis And O. Bonariensis (Atheriniformes), Lily Hughes, Gustavo Somoza, Bryan Nguyen, James Bernot, Mariano Gonzalez-Castro, Juan Martin Diaz de Astarloa, Guillermo Orti 2017 George Washington University

Transcriptomic Differentiation Underlying Marine‐To‐Freshwater Transitions In The South American Silversides Odontesthes Argentinensis And O. Bonariensis (Atheriniformes), Lily Hughes, Gustavo Somoza, Bryan Nguyen, James Bernot, Mariano Gonzalez-Castro, Juan Martin Diaz De Astarloa, Guillermo Orti

Computational Biology Institute

Salinity gradients are critical habitat determinants for freshwater organisms. Silverside fishes in the genus Odontesthes have recently and repeatedly transitioned from marine to freshwater habitats, overcoming a strong ecological barrier. Genomic and transcriptomic changes involved in this kind of transition are only known for a few model species. We present new data and analyses of gene expression and microbiome composition in the gills of two closely related silverside species, marine O. argentinensis and freshwater O. bonariensis and find more than three thousand transcripts differentially expressed, with osmoregulatory/ion transport genes and immune genes showing very different expression patterns across species ...


Ivd Formation In Monodelphis Domestica, Emma C.B. Gray, Jen Maier, Karen Sears 2017 Parkland College

Ivd Formation In Monodelphis Domestica, Emma C.B. Gray, Jen Maier, Karen Sears

Phenotypic Plasticity Research Experience for Community College Students

The research summarized on this poster supports the use of the gray short-tailed opossum (Monodelphis domestica) as a lab animal for research into invertebral disc formation and degeneration. Preliminary data suggests that some genes involved in the formation and maintenance of the notochord in mice are the same in the short-tailed opossum. Data also suggests the ossification of the vertebral column of pups proceeds anteriorly to posteriorly and that much of the maturation of the nucleus puposus happens in the first ten days.


Candida And Pseudomonas Interact To Enhance Mucosal Infection In Transparent Zebrafish, Audrey C. Bergeron 2017 The University of Maine

Candida And Pseudomonas Interact To Enhance Mucosal Infection In Transparent Zebrafish, Audrey C. Bergeron

Electronic Theses and Dissertations

Polymicrobial communities exist throughout the human body and include both fungi and bacteria. During disease, cross-kingdom interactions among bacteria, fungi, and/or the immune system can alter virulence and lead to complex polymicrobial infections. The fungus C. albicans is among the most commonly isolated fungi in the context of fungal-bacterial co-infections and is often accompanied by the bacterium P. aeruginosa at a variety of sites throughout the body including mucosal tissues such as the lung. In vitro, C. albicans and P. aeruginosa have a cyclic, bi-directional, and largely antagonistic relationship, but these interactions do not account for the role of ...


Letter -- Re: Biomedical Experiments Involving Non-Human Primates, Jarrod Bailey 2017 Cruelty Free International

Letter -- Re: Biomedical Experiments Involving Non-Human Primates, Jarrod Bailey

Jarrod Bailey, Ph.D.

One of the main motivations for my 2016 review was my frustration at the repeated, unsound case put forth by many of those who advocate for continued, and greater, invasive neuroscience on our primate cousins. In my view, this case is multiply and seriously flawed. It overlooks the opposition of the public to animal experiments, in particular to NHP neuroscience; it skews the balance of associated harms and proposed benefits by understating the former and overstating the latter; it assumes a default human relevance without critically questioning this assumption and without appreciating the abundant evidence against it; it plays down ...


Finding A Link Between Circadian Rhythms And The Immune System Of Captive Zebra Finches (Taeniopygia Guttata), Amber Bishop 2017 Western Kentucky University

Finding A Link Between Circadian Rhythms And The Immune System Of Captive Zebra Finches (Taeniopygia Guttata), Amber Bishop

Honors College Capstone Experience/Thesis Projects

Circadian rhythms are commonplace in organisms and are normally controlled by a master clock. More recent evidence suggests that autonomous clocks operate in various systems in the body, including the immune system. This study looks for such a connection between the circadian rhythm and the immune system. In this study, captive zebra finches (Taeniopygia guttata) were exposed to different light and dark cycles and blood samples were taken every six hours. Birds were exposed to 12 hours of light and then 12 hours of darkness (LD), 24 hours of darkness (DD), or 48 hours of DD. After collecting whole blood ...


Evaluating The Social Behavior And Activity Patterns Of Clouded Leopards (Neofelis Nebulosa) At The Nashville Zoo: Research And Literature Review, Meghan Ryckeley 2017 Western Kentucky University

Evaluating The Social Behavior And Activity Patterns Of Clouded Leopards (Neofelis Nebulosa) At The Nashville Zoo: Research And Literature Review, Meghan Ryckeley

Honors College Capstone Experience/Thesis Projects

The behavior of the elusive clouded leopard (Neofelis nebulosa) is not well understood. In captivity, they are prone to exhibit stress-related behaviors and are often aggressive to conspecifics when paired. The present study built on past research by examining clouded leopards at the Nashville Zoo at Grassmere (NZG) to determine their activity patterns and temperament. Twelve leopards were exposed to familiar and unfamiliar objects and people, as well as their reflection in a mirror over nine trials. The males were often more reactive than the females, and the 5-year olds more reactive than their younger counterparts. The mirror image and ...


Analysis Of Population Structure In A California Newt (Taricha Torosa) Metapopulation, Jessica Vincent 2017 Western Kentucky University

Analysis Of Population Structure In A California Newt (Taricha Torosa) Metapopulation, Jessica Vincent

Honors College Capstone Experience/Thesis Projects

As anthropogenic influences take an ever-increasing toll on the environment, understanding how environmental change affects species is paramount. Concern regarding decline in amphibian populations has spurred research examining the effects of habitat change on the dynamics of populations at landscape levels. One important goal is to understand how gene flow among populations is affected by changes in habitat. Biologists need to consider the relationship between gene flow and habitat alterations so that movements among individual breeding ponds can be maintained over time, reducing risk of local extinction events. This study focuses on patterns of gene flow among thirteen populations of ...


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