Superresolution Imaging Identifies That Conventional Trafficking Pathways Are Not Essential For Endoplasmic Reticulum To Outer Mitochondrial Membrane Protein Transport., 2017 George Washington University
Superresolution Imaging Identifies That Conventional Trafficking Pathways Are Not Essential For Endoplasmic Reticulum To Outer Mitochondrial Membrane Protein Transport., Kyle Salka, Shivaprasad Bhuvanendran, Kassandra Wilson, Petros Bozidis, Mansi Mehta, Kristin Rainey, Hiromi Sesaki, George H Patterson, Jyoti K. Jaiswal, Anamaris M. Colberg-Poley
Integrative Systems Biology Faculty Publications
Most nuclear-encoded mitochondrial proteins traffic from the cytosol to mitochondria. Some of these proteins localize at mitochondria-associated membranes (MAM), where mitochondria are closely apposed with the endoplasmic reticulum (ER). We have previously shown that the human cytomegalovirus signal-anchored protein known as viral mitochondria-localized inhibitor of apoptosis (vMIA) traffics from the ER to mitochondria and clusters at the outer mitochondrial membrane (OMM). Here, we have examined the host pathways by which vMIA traffics from the ER to mitochondria and clusters at the OMM. By disruption of phosphofurin acidic cluster sorting protein 2 (PACS-2), mitofusins (Mfn1/2), and dynamin related protein 1 ...
Measuring The Presence Of The Amphibian Pathogen Batrachochytrium Dendrobatidis In East Tennessee, 2017 University of Tennessee at Chattanooga
Measuring The Presence Of The Amphibian Pathogen Batrachochytrium Dendrobatidis In East Tennessee, Erin A. Schrenker
I examined the presence and absence of the amphibian pathogen Batrachochytrium dendrobatidis in three survey areas in Eastern Tennessee. Amphibians were swabbed to assess for Bd and other ecological qualities like canopy cover, and air and water temperature are a few of the measurements evaluated. DNA was extracted from the swabs using a Qiagen DNeasy Blood and Tissue Kit and samples were PCRed in triplicate to confirm presence or absence. All 48 animals that were swabbed in the field were negative for Bd. These results parallel other findings that few Bd positive assays have been conducted in East Tennessee from ...
Modelling The Role Of Chance In Mating Dynamics, 2017 University of Tennessee at Chattanooga
Modelling The Role Of Chance In Mating Dynamics, Libby K. Boykin
This undergraduate honors thesis examines the role of chance in mating dynamics, focusing on the popular measures of Is and OSR and whether or not they are accurate measures of sexual selection. The first chapter is an overview of sexual selection and how biologists typically measure it, and the second chapter describes the modelling scenarios we created to assess the interaction of Is and OSR with both chance and parental investment. Our results show that Is and OSR have indeed been used incorrectly, and that we should shift our focus to trait-based methods when measuring sexual selection.
Prevalence Of Batrachochytrium Dendrobatidis (Bd) In Watercourses Situated In Southeast Tennessee, 2017 University of Tennessee at Chattanooga
Prevalence Of Batrachochytrium Dendrobatidis (Bd) In Watercourses Situated In Southeast Tennessee, Macall A. Nabors
Four hundred and twenty-seven amphibian species are critically endangered. The fungal disease Batrachochytrium dendrobatidis (Bd) has been correlated with amphibian decline and extinction. Numerous studies concerning the nature of Bd have been conducted; however, additional studies are needed to adequately evaluate Bd in southeastern Tennessee. As a result of previous studies, the following hypothesizes were proposed: watercourses in southeastern Tennessee will test positive for Bd, non-impacted streams will have a greater presence of Bd than impacted streams, and increased canopy coverage has a positive correlation with the presence of Bd. Field sampling took place from June 2nd 2016 to August ...
Inclusive Teaching Strategies: An Evaluation Of Course Structure And Summative Assessment In Introductory Biology, 2017 East Tennessee State University
Inclusive Teaching Strategies: An Evaluation Of Course Structure And Summative Assessment In Introductory Biology, Oluwaseun O. Agboola
Electronic Theses and Dissertations
Several active learning strategies have been used when increasing the structure of a course as increasing course structure has been known to improve student learning in introductory STEM courses. Much has been studied on the value of frequent formative assessment; however, few studies have evaluated the effective modes of delivering summative assessment. This study examines the use of summative assessment as an inclusive teaching practice to improve first generation college student success in introductory biology and also uses faculty surveys to find out how instructors structure their introductory biology course and why they are structured that way. Final exams were ...
Molecular And Physiological Mechanisms Of Toxin Resistance In Toad-Eating Snakes, 2017 Utah State University
Molecular And Physiological Mechanisms Of Toxin Resistance In Toad-Eating Snakes, Shabnam Mohammadi
All Graduate Theses and Dissertations
Many plants and animals are defended by toxic compounds, and circumvention of those defenses often has involved the evolution of elaborate mechanisms for tolerance or resistance of the toxins. Toads synthesize potent cardiotonic steroids known as bufadienolides (BDs) from cholesterol and store those toxins in high concentrations in their cutaneous glands. Those toxins protect toads from the majority of predators, including most snakes that readily consume other species of frogs. BDs exert their effect by inhibiting ion transport by the Na+/K+-ATPase (NKA). This ubiquitous transmembrane enzyme consists of a catalytic alpha-subunit, which carries out the enzyme's functions ...
Variation In Density Dependent Seedling Survival Across Forests Of Different Successional Age And Hunting Protection Status, 2017 University of Nebraska-Lincoln
Variation In Density Dependent Seedling Survival Across Forests Of Different Successional Age And Hunting Protection Status, Nohemi Huanca-Nunez
Dissertations and Theses in Biological Sciences
Over 50% of the original extent of tropical forests has been cleared. Restoration of secondary forests is important for maintaining the ecosystem services that mature tropical forests provide. Density dependence (DD) is thought to be a major mechanism for shaping forest community structure and may cause reduced spatial aggregation among individuals of the same species, allowing for maintenance of diversity. While much research has focused on DD in mature tropical forests, few studies have examined how DD may influence community structure in secondary forests, many of which are also exposed to hunting. There are several important agents of negative and ...
Neural Expression And Function Of Autism Candidate Genes In Zebrafish, 2017 University of Texas at Tyler
Neural Expression And Function Of Autism Candidate Genes In Zebrafish, Elyse P. Kite
The Autism Spectrum Disorders (ASD) refer to a large heterogeneous set of developmental disorders defined by two behavioral domains: 1) Impairment of social comprehension and abnormal development of social communication, and 2) the presence of stereotypical and repetitive behaviors. The etiology of ASD is complex, with both genetic and environmental components. De novo and inherited mutations within DPP6 and PSMD12 have been identified within individuals diagnosed with ASD suggesting an etiologic role. Understanding the functionality of these genes could lead novel treatments for ASD. Zebrafish were utilized as a model system for the assessment of functionality of the orthologous genes ...
Cloning And Expression Of Pseudomonas Aeruginosa Elastase For Elucidating The Pathophysiological Effect On Lung Tissue, 2017 University of Texas at Tyler
Cloning And Expression Of Pseudomonas Aeruginosa Elastase For Elucidating The Pathophysiological Effect On Lung Tissue, Derek T. Draper
Pseudomonas aeruginosa causes acute nosocomial as well as community acquired pneumonia. In cystic fibrosis patients, this organism causes recurrent infections and inflammation with a high rate of morbidity and mortality. To support research in elucidating the molecular mechanisms of Pseudomonas elastase (PE) on disease progression, PE was expressed in E. coli and the protein purified for further application. This study represents a valid experimental protocol to produce. Genomic DNA was isolated from P. aeruginosa and selected regions were amplified using PCR with lasB gene specification. Amplified DNA was ligated into plasmids and E. coli were transformed to support the recombinant ...
The Role Of Tributaries In Structuring Mussel Communities, 2017 University of Texas at Tyler
The Role Of Tributaries In Structuring Mussel Communities, Cassie M. Vaughan
Mussel species composition in tributaries compared to the river mainstem, and the influence of tributaries on the river mainstem of mussel communities, are poorly understood. This paper aims to understand differences in mussel habitat associations between tributaries and the Neches River mainstem and if the mussel communities are nested in tributaries. During the months June through October in 2016, 28 tributaries and 22 mainstem sites were surveyed in the Neches River using tactile time search. A total of 3,620 individuals were collected, of which 44.5% were collected from tributaries. However, only eight of the 28 tributaries contained mussels ...
Transcriptomic Analysis Of Maternally Provisioned Cues For Phenotypic Plasticity In The Annual Killifish, Austrofundulus Limnaeus, Amie L. Romney, Jason E. Podrabsky
Biology Faculty Publications and Presentations
Background: Genotype and environment can interact during development to produce novel adaptive traits that support life in extreme conditions. The development of the annual killifsh Austrofundulus limnaeus is unique among vertebrates because the embryos have distinct cell movements that separate epiboly from axis formation during early development, can enter into a state of metabolic dormancy known as diapause and can survive extreme environmental conditions. The ability to enter into diapause can be maternally programmed, with young females producing embryos that do not enter into diapause. Alternately, embryos can be programmed to “escape” from diapause and develop directly by both maternal ...
Compromised External Validity: Federally Produced Cannabis Does Not Reflect Legal Markets, Daniela Vergara, L. Cinnamon Bidwell, Steep Hill, Kent E. Hutchison, Nolan C. Kane
University Libraries Open Access Fund Supported Publications
As the most widely used illicit drug worldwide, and as a source of numerous under-studied pharmacologically-active compounds, a precise understanding of variability in psychological and physiological effects of Cannabis varieties is essential. The National Institute on Drug Abuse (NIDA) is designated as the sole legal producer of Cannabis for use in US research studies. We sought to compare the chemical profiles of Cannabis varieties that are available to consumers in states that have statelegalized use versus what is available to researchers interested in studying the plant and its effects. Our results demonstrate that the federally-produced Cannabis has significantly less variety ...
Determination Of The Binding Site Of Adenovirus E4 11k On The Cellular Protein Ddx6, 2017 Georgia College and State University
Determination Of The Binding Site Of Adenovirus E4 11k On The Cellular Protein Ddx6, Clint E. Edmunds, Courtney F. Moon
Georgia College Student Research Events
Adenovirus is a double-stranded DNA virus that is responsible for localized infections, such as upper respiratory tract infections. The virus takes over the target cell through many mechanisms, in particular taking control of host cell gene expression mechanisms as well as controlling host cell protein synthesis machinery. One of the functions of the adenovirus E4 11k protein is in turning off host cell protein synthesis and regulating late viral gene expression. E4 11k from all adenovirus subclasses has been shown to disrupt cellular RNA processing bodies (P-bodies), and adenovirus serotype 5 has a direct interaction with a P-body protein, Ddx6 ...
Characterization Of A Neuroprotective Retinal Synapse After Inducing Glaucoma In Long Evans Rats, 2017 Western Michigan University
Characterization Of A Neuroprotective Retinal Synapse After Inducing Glaucoma In Long Evans Rats, Cynthia A. Cooley-Themm
Research and Creative Activities Poster Day
Glaucoma is a group of degenerative retinal diseases characterized by progressive loss of retinal ganglion cells (RGCs) and their axons leading to irreversible loss of vision. Neuroprotection has been defined as any intervention that prevents optic nerve damage or RGC death. The neurotransmitter, acetycholine (ACh), has been linked to neuroprotection against excitotoxic cell death and neurodegenerative diseases of the central nervous system (CNS). Results from our lab have demonstrated that ACh provides neuroprotection against glutamate-induced excitotoxicity in isolated pig and rat RGCs in vitro. Additional studies using an in vivo rat model of glaucoma have shown that intravitreal injections or ...
An Electrochemical Analysis Of Fretting Corrosion In Metal-On-Metal Hip Implants Subjected To High Impaction Loads, Joe Morin, Timothy L. Norman, Thomas K. Fehring
The Research and Scholarship Symposium
The metal-on-metal total hip arthroplasty, a procedure where the hip joint is replaced by a femoral prosthesis with a metal femoral head and a metal socket, has been a popular option for patients requiring a hip joint replacement. Metal on metal hip implants have been a successful implant design until recently where there has been an increased number of failures of this type of implant due to fretting corrosion, believed to be caused from the use of large femoral heads. Fretting corrosion in hip implants results from cyclic micromotion at the taper-trunnion interface; this interface motion removes the protective oxidation ...
3d Printing Of Biodegradable Scaffolds For Tissue Engineering Applications, 2017 Cedarville University
3d Printing Of Biodegradable Scaffolds For Tissue Engineering Applications, Joe Morin, Michael Pickett, Amy Abraham, Tiera Martinelli
The Research and Scholarship Symposium
With the recent improvements in three dimensional (3D) printing technologies, the potential for tissue engineering and regenerative medicine have significantly improved. One key idea in tissue engineering is to specifically design scaffolds to aid in the healing process by being incorporated into the body’s own tissue. The overall goal of this project is to investigate 3D printable scaffold design to access suitability for tissue replacement. This was accomplished by analyzing the effect of the material used to create the scaffolds, pore size, and pore shape on mechanical stiffness and cell culturability. Based on published literature, it was determined that ...
Migration Frustrations Of Mir-146a Regulation, 2017 Cedarville University
Migration Frustrations Of Mir-146a Regulation, Emma Baccus, Victoria Gahman, Hannah Phillips, Shannon Rappaport, Alyssa Reiter, Kaleb M. Pauley
The Research and Scholarship Symposium
The autoimmune disease, Sjögren’s Syndrome (SS), causes the degradation of salivary and lacrimal glands due to an influx of immune cells. In previous studies, a significant increase in miR-146a was observed in the peripheral blood mononuclear cells of SS patients. Since immune cell infiltration is critical in SS pathogenesis, the following research examines the effect of miR-146a on cell migration. We hypothesize that transfecting THP-1 human monocytes with synthetic miR-146a will downregulate migration of the monocytes based on other studies stating that miR-146a downregulates migration in vivo. In order to execute our experiment, we transfected THP-1 cells with ...
Mir-146a Upregulation Of Phagocytosis In Human Macrophages, 2017 Cedarville University
Mir-146a Upregulation Of Phagocytosis In Human Macrophages, Ryan Marquardt, Daniel J. Stank, Kaleb M. Pauley
The Research and Scholarship Symposium
Sjögrens Syndrome (SjS) is an autoimmune disease that attacks exocrine glands such as salivary and lacrimal glands resulting in severe dryness of the mouth and eyes. Previous studies have linked increased microRNA-146a (miR-146a) expression in peripheral blood mononuclear cells in SjS patients compared to healthy controls. MicroRNAs (miRNAs), small non-coding RNA molecules that post-transcriptionally regulate gene expression, are known to play key regulatory roles in immune responses and have been implicated in a growing number of autoimmune disorders. Further investigation into the role of increased miR-146a expression in SjS revealed links to several immune functions including phagocytosis.Our goal was ...
Effect Of Metformin On Mir-146a Expression, 2017 Cedarville University
Effect Of Metformin On Mir-146a Expression, S. Jackson Grout, Bryce C. Macturk, Amanda D. Sims, Kaleb M. Pauley
The Research and Scholarship Symposium
Sjögren’s Syndrome (SjS) is an autoimmune disorder that affects secretory glands in the human body, restricting their function and causing extreme dryness in areas like the mouth and eyes. miR-146a is an anti-inflammatory microRNA that targets the NFκB activation pathway. Previous studies have shown that SjS patients have increased miR-146a expression, despite having high levels of inflammation. The objective of this study was to investigate whether metformin, a diabetes drug with a wide variety of effects and potential functions, reduces levels of miR-146a expression. Metformin is known to reduce inflammation by inhibiting the activation of NFκB. THP-1 human ...
Cichlid Behavioral Ecology: Streams Of Costa Rica, Ali Saheb, Erfan Ranjbar, Shannon Dieffenderfer, Yazmeen Almutairri, Nicole Williams-Ugula, Ella Brook Gray, Adel Pereira
Georgia State Undergraduate Research Conference
No abstract provided.