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Generation And Assessment Of Muscular Mutations In Caenorhabditis Elegans, Katie N. Reget 2015 DePaul University

Generation And Assessment Of Muscular Mutations In Caenorhabditis Elegans, Katie N. Reget

DePaul Discoveries

A study of egg laying muscular mutations in C. elegans was conducted over a span of ten weeks. Parent (EGL-19) and wild type (N2) were exposed to mutagenesis and integration mutation techniques to generate genetic and physical different mutants. Overall, four genetic, physical and phenotypically unique worms were generated for the process of mutagenesis. The worms used in the process of integration were found to have shortened life spans, reduced size and decrease numbers of progeny.


Chronic Inflammation As A Result Of Hepatitis C Virus Infection: A Review Of The Literature, Samantha L. Lane 2015 DePaul University

Chronic Inflammation As A Result Of Hepatitis C Virus Infection: A Review Of The Literature, Samantha L. Lane

DePaul Discoveries

Approximately 170 million people are infected with Hepatitis C virus (HCV) worldwide5,6. It is estimated that roughly 80% of those infected suffer from persistent infection with the virus; this persistence of infection is progressive, and over time can lead to fibrosis, cirrhosis, and hepatocellular carcinoma7. Chronic inflammation and apoptotic deregulation are both hallmarks of chronic HCV infection, and many molecular pathways are initiated in both the innate and adaptive immune responses during infection with this viral pathogen. The aim of this review was to survey some of the major molecular mechanisms responsible for the induction of chronic ...


Functional Analysis Of The Molluscum Contagiosum Virus Mc160 Death Effector Domain-Containing Protein Rxdl Motif, Sarah Weber 2015 Seton Hall University

Functional Analysis Of The Molluscum Contagiosum Virus Mc160 Death Effector Domain-Containing Protein Rxdl Motif, Sarah Weber

Seton Hall University Dissertations and Theses (ETDs)

The Molluscum contagiosum virus (MCV) is a member of the Poxviridae family that causes benign skin lesions. MCV lesions persist on average for 8-12 months in otherwise healthy individuals. MCV lesions are characterized by reduced inflammation. The persistence and reduction of inflammation at the site of MCV lesions have been attributed to MCV immune evasion genes. MCV encodes two death effector domain (DED) containing proteins, MC159 and MC160. DEDs are found in cellular proteins such as FADD and procaspase-8. These cellular proteins are involved in several innate immune responses such as apoptosis and activation of interferon (IFN). Presumably, MC159 and ...


The C-Terminal Linker Of Ftsz Acts As An Intrinsically Disordered Peptide During Cell Division In Bacillus Subtilis, Steven Grigsby 2015 Washington University in St Louis

The C-Terminal Linker Of Ftsz Acts As An Intrinsically Disordered Peptide During Cell Division In Bacillus Subtilis, Steven Grigsby

Undergraduate Theses—Unrestricted

The bacterial tubulin homologue FtsZ polymerizes in vitro in a GTP-dependent manner to form long, single stranded filaments. In cells, these filaments assemble at the nascent division site, interacting laterally to form the contractile Z ring. The Z ring serves as a scaffold for the rest of the division machinery and constricts at the leading edge of the invaginating septum during cytokinesis. The FtsZ polypeptide consists of three primary domains: the N-terminal globular core consisting of 315 residues that contains the GTP binding site, a variable and flexible C-terminal linker (CTL) consisting of 50 residues, and a conserved region at ...


Cave Cyanobacteria Showing Antibacterial Activity, Vasiliki Lamprinou, Kyriaki Tryfinopoulou, Emmanuel N. Velonakis, Alkiviadis Vatopoulos, Smaragdi Antonopoulou, Elizabeth Fragopoulou, Adriani Pantazidou, Athena Economou-Amilli 2015 University of Athens, Faculty of Biology, Department of Ecology & Systematics

Cave Cyanobacteria Showing Antibacterial Activity, Vasiliki Lamprinou, Kyriaki Tryfinopoulou, Emmanuel N. Velonakis, Alkiviadis Vatopoulos, Smaragdi Antonopoulou, Elizabeth Fragopoulou, Adriani Pantazidou, Athena Economou-Amilli

International Journal of Speleology

Cave Cyanobacteria - thriving in an ‘extreme’ environment with interesting species biodiversity - are supposed to be a potential source of bioactive compounds. Lipid extracts from pure cultures of two recently established Cyanobacteria from Greek caves, Toxopsis calypsus and Phormidium melanochroun, were used for antibacterial screening against human pathogenic bacteria (reference and clinical isolates). Antimicrobial Susceptibility testing for both taxa was carried out using the disc-diffusion (Kirby Bauer) method, while preliminary data applying the standard broth microdilution method for the determination of the Minimal Inhibitory Concentration (MIC) are given only for T. calypsus. Antibacterial activity was demonstrated against the Gram-positive clinical and ...


Human And Murine Clonal Cd8+ T Cell Expansions Arise During Tuberculosis Because Of Tcr Selection, Claudio Nunes-Alves, Matthew G. Booty, Stephen M. Carpenter, Alissa C. Rothchild, Constance J. Martin, Danielle Desjardins, Katherine Steblenko, Henrik N. Kløverpris, Rajhmun Madansein, Duran Ramsuran, Alasdair Leslie, Margarida Correia-Neves, Samuel M. Behar 2015 University of Massachusetts Medical School Worcester

Human And Murine Clonal Cd8+ T Cell Expansions Arise During Tuberculosis Because Of Tcr Selection, Claudio Nunes-Alves, Matthew G. Booty, Stephen M. Carpenter, Alissa C. Rothchild, Constance J. Martin, Danielle Desjardins, Katherine Steblenko, Henrik N. Kløverpris, Rajhmun Madansein, Duran Ramsuran, Alasdair Leslie, Margarida Correia-Neves, Samuel M. Behar

Microbiology and Physiological Systems Publications and Presentations

The immune system can recognize virtually any antigen, yet T cell responses against several pathogens, including Mycobacterium tuberculosis, are restricted to a limited number of immunodominant epitopes. The host factors that affect immunodominance are incompletely understood. Whether immunodominant epitopes elicit protective CD8+ T cell responses or instead act as decoys to subvert immunity and allow pathogens to establish chronic infection is unknown. Here we show that anatomically distinct human granulomas contain clonally expanded CD8+ T cells with overlapping T cell receptor (TCR) repertoires. Similarly, the murine CD8+ T cell response against M. tuberculosis is dominated by TB10.44-11-specific T cells ...


Effect Of Cmvil-10 On Exosome Production By Human Breast Cancer Cells, Susanna N. Basappa 2015 University of San Francisco

Effect Of Cmvil-10 On Exosome Production By Human Breast Cancer Cells, Susanna N. Basappa

Undergraduate Honors Theses

Human cytomegalovirus (HCMV) is a ubiquitous virus that infects 70-90% of the general population, primarily the immunocompromised, but has been implicated in several forms of cancer, including breast cancer. Breast cancer is the second leading cause of cancer related deaths in women in North America, usually from metastasis. Exosomes are 30-100nm vesicles produced by most cells which carry protein and RNA to cells in their microenvironment. The aim of this study is to investigate the impact of HCMV-infection of a secreted viral cytokine, cmvIL-10, on exosome production by highly metastatic breast cancer cells.

MDA-MB-231 cells were cultured in vitro, and ...


Characterization And Fluorescence Of Yellow Biofilms In Karst Caves, Southwest Slovenia, Janez Mulec, Andreea Oarga-Mulec, Rok Tomazin, Tadeja Matos 2015 Karst Research Institute, Research Centre of the Slovenian Academy of Sciences and Arts

Characterization And Fluorescence Of Yellow Biofilms In Karst Caves, Southwest Slovenia, Janez Mulec, Andreea Oarga-Mulec, Rok Tomazin, Tadeja Matos

International Journal of Speleology

Biofilms of different colours that colonize surfaces within karst caves represent a source of nutrients. They occur commonly and abundantly at sites with sediments, and close to seepages or underground rivers. Golden-yellow subaerial biofilms are particularly well observed because of their contrast with their surroundings, the characteristics of the pigment and recently, even more, due to the characteristics of light-emitting diode (LED) illumination. Yellow microbial biofilms were sampled from three caves in southwestern Slovenia, Dimnice, Križna jama and Sveta jama. The highest concentration of cultivable microbes (2.33×108 CFU/g) and the biggest number of identified bacteria (66 ...


Molecular Epidemiology, Genotyping, And Mat Testing Of Toxoplasma Gondii, Jack Kang 2015 University of Tennessee, Knoxville

Molecular Epidemiology, Genotyping, And Mat Testing Of Toxoplasma Gondii, Jack Kang

University of Tennessee Honors Thesis Projects

No abstract provided.


Positive Regulation Of Localization Of Cell Division Proteins In Escherichia Coli, William P. Deaderick 2015 University of Tennessee, Knoxville

Positive Regulation Of Localization Of Cell Division Proteins In Escherichia Coli, William P. Deaderick

University of Tennessee Honors Thesis Projects

No abstract provided.


Characterization And Investigation Of Fungi Inhabiting The Gastrointestinal Tract Of Healthy And Diseased Humans, Mallory J. Suhr 2015 University of Nebraska-Lincoln

Characterization And Investigation Of Fungi Inhabiting The Gastrointestinal Tract Of Healthy And Diseased Humans, Mallory J. Suhr

Dissertations & Theses in Food Science and Technology

Gastrointestinal microbiome studies have failed to include fungi in total community analyses. As a result, their diversity and function in the gut is poorly understood. Recent work has begun to uncover the role intestinal fungi play in diet, immune system development, interactions with other microorganisms in the gut, and pathogenesis of diseases. Advances in sequencing technologies allow for the ability to profile the fungal gut microbiome (“mycobiome”) in healthy and diseased states. This thesis explores the mycobiome in 1) healthy humans with a vegetarian diet and 2) pediatric small bowel transplant recipients that develop fungal bloodstream infections.

The gut mycobiome ...


A Two-Component Regulatory System Associated With Scytonemin Biosynthesis In Nostoc Punctiforme Atcc 29133, Jacob P. Janssen 2015 Indiana University - Purdue University Fort Wayne

A Two-Component Regulatory System Associated With Scytonemin Biosynthesis In Nostoc Punctiforme Atcc 29133, Jacob P. Janssen

Masters' Theses

As phototrophic bacteria, cyanobacteria are continually exposed to ultraviolet radiation as they harvest solar energy. In particular, long-wavelength ultraviolet radiation (UVA) damages living cells by releasing reactive oxygen species. To mitigate damage to the cell, some cyanobacteria produce a UVA-absorbing pigment in the extracellular sheath, known as scytonemin. Scytonemin is a heterocyclic, dimeric molecule that is only produced upon induction by UVA. In Nostoc punctiforme ATCC 29133, it is hypothesized that scytonemin is regulated by the two component regulatory system (TCRS) of NpF1277 (sensor kinase) and NpF1278 (response regulator). Gene expression of the TCRS was studied after exposure to UVA ...


Characterization Of Seed Transmission Of Soybean Mosaic Virus In Soybean, Tanvir Bashar 2015 The University of Western Ontario

Characterization Of Seed Transmission Of Soybean Mosaic Virus In Soybean, Tanvir Bashar

University of Western Ontario - Electronic Thesis and Dissertation Repository

Infection by Soybean mosaic virus (SMV) is recognized as a serious, long-standing threat in most soybean (Glyince max (L.)Merr.) producing areas of the world. The aim of this work was to understand how SMV transmits from infected soybean maternal tissues to the next generation by investigating the possible routes and amounts of seed transmission of SMV. Analysis of seeds at various reproductive stages revealed that SMV infects all parts of the seed, including the embryo, cotyledon and testa. In situ hybridization and immunofluorescence studies detected the presence of negative sense RNA and dsRNA in the suspensor base regions and ...


How Does The Exploitation And Degradation Of Finite Natural Resources By Industrial Oil Producers Effect Our Future Planetary Environment?, Zachary J. King Mr. 2015 Kennesaw State University

How Does The Exploitation And Degradation Of Finite Natural Resources By Industrial Oil Producers Effect Our Future Planetary Environment?, Zachary J. King Mr.

Symposium of Student Scholars

Zachary King

Dr. Tu

26 March 2015

GEOG 3700

Abstract

The purpose of this presentation is to communicate content of the reports given by credible institution on the degradation and exploitation of finite natural resources by corporate institutions, for short term financial gain, and how it will effect the future planetary environment. Methods used in obtaining this research range from point source observation directly from oil spills in the Gulf or pipe leaks in the Mid-West, to analytical and numerical data quantifications gathered over a period of two decades. This presentation encompasses a wide scope of information concerning the way ...


The C-Terminal Linker Of Ftsz Acts As An Intrinsically Disordered Peptide During Cell Division In Bacillus Subtilis, Steven Grigsby 2015 Washington University in St Louis

The C-Terminal Linker Of Ftsz Acts As An Intrinsically Disordered Peptide During Cell Division In Bacillus Subtilis, Steven Grigsby

Undergraduate Research Symposium Posters & Abstracts

The bacterial tubulin homologue FtsZ polymerizes in vitro in a GTP-dependent manner to form long, single stranded filaments. In cells, these filaments assemble at the nascent division site, interacting laterally to form the contractile Z-ring, which serves as a scaffold for the rest of the division machinery and constricts at the leading edge of the invaginating septum during cytokinesis. FtsZ consists of three primary domains: the N-terminal globular core consisting of 315 residues that contains the GTP-binding site, a variable and flexible C-terminal linker (CTL) consisting of 50 residues, and a conserved region at the C-terminus consisting of 17 residues ...


Isolation And Comparative Genomic Analysis Of Mycobacteriophage Enkatz, Thomas Van Horn, Micah Rickles-Young, Shaarada Srivasta, Tina Zudock 2015 Washington University in St Louis

Isolation And Comparative Genomic Analysis Of Mycobacteriophage Enkatz, Thomas Van Horn, Micah Rickles-Young, Shaarada Srivasta, Tina Zudock

Thomas Van Horn

Phage Enkatz is a temperate mycobacteriophage isolated from an un-enriched soil sample collected from the South Forty housing area of the Washington University in St. Louis campus. Enkatz displays unequally sized plaques with a clear center that become cloudier with radial distance from the center. Genome analysis indicates that Enkatz is a cluster A1 mycobacteriophage with a genome size of 49,738 bases and 82 identified genes, 33 of which have been assigned functions. This analysis reveals that the majority of the genes in the positive strand code for structural proteins, while the majority of the genes in the negative ...


Analysis Of Activation Of The Nf-Κb Pathway By Shfv Infection, Ashley Aisabor 2015 Georgia State University

Analysis Of Activation Of The Nf-Κb Pathway By Shfv Infection, Ashley Aisabor

Georgia State Undergraduate Research Conference

No abstract provided.


Complex Eukaryotic Protein Expression Using A Prokaryotic Chassis (Escherichia Coli), Derrica Burke, Julia Dave, Jasmine Padalla, Tre Landry 2015 Georgia State University

Complex Eukaryotic Protein Expression Using A Prokaryotic Chassis (Escherichia Coli), Derrica Burke, Julia Dave, Jasmine Padalla, Tre Landry

Georgia State Undergraduate Research Conference

No abstract provided.


Preliminary Report On The Production Of A Shiga-Like Toxoid Fusion Protein As Associated With Novel Flic Vaccine, Michael Maulin 2015 Georgia State University

Preliminary Report On The Production Of A Shiga-Like Toxoid Fusion Protein As Associated With Novel Flic Vaccine, Michael Maulin

Georgia State Undergraduate Research Conference

No abstract provided.


Bone Marrow Stromal Antigen 2 (Bst-2) Dna Is Demethylated In Breast Tumors And Breast Cancer Cells, Wadie D. Mahauad-Fernandez, Nicholas C. Borcherding, Weizhou Zhang, Chioma M. Okeoma 2015 University of Iowa

Bone Marrow Stromal Antigen 2 (Bst-2) Dna Is Demethylated In Breast Tumors And Breast Cancer Cells, Wadie D. Mahauad-Fernandez, Nicholas C. Borcherding, Weizhou Zhang, Chioma M. Okeoma

Department of Microbiology Publications

BACKGROUND: Bone marrow stromal antigen 2 (BST-2) is a known anti-viral gene that has been recently identified to be overexpressed in many cancers, including breast cancer. BST-2 is critical for the invasiveness of breast cancer cells and the formation of metastasis in vivo. Although the regulation of BST-2 in immune cells is unraveling, it is unknown how BST-2 expression is regulated in breast cancer. We hypothesized that meta-analyses of BST-2 gene expression and BST-2 DNA methylation profiles would illuminate mechanisms regulating elevated BST-2 expression in breast tumor tissues and cells.

MATERIALS AND METHODS: We performed comprehensive meta-analyses of BST-2 gene ...


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