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Assessing Selective Export Of Fluorescent Ssa4 Reporters, Caroline Firmin, Rebecca Adams 2022 Belmont University

Assessing Selective Export Of Fluorescent Ssa4 Reporters, Caroline Firmin, Rebecca Adams

Science University Research Symposium (SURS)

The process of creating proteins from mRNA is necessary for cell viability. Importantly, the proteins that are generated are differentially regulated so that the cell makes specific proteins in response to its environment. For example, mRNA is selectively exported during stress conditions to allow response to this stress. During heat shock at 42°C, in which proteins are denatured, the gene SSA4 encodes a selectively transported protein chaperone that allows refolding of denatured proteins. This study sought to explore the mechanisms of this selective export. To do this, I analyzed the expression of a fluorescent reporter wherein GFP is expressed ...


Comparison Of Kanamycin-Resistant Bacteria In Earthworm Digestive Tracts And Soil From Compost Bins, Samantha Dooyema, Martin Dolan, Abigal Morse 2022 Belmont University

Comparison Of Kanamycin-Resistant Bacteria In Earthworm Digestive Tracts And Soil From Compost Bins, Samantha Dooyema, Martin Dolan, Abigal Morse

Science University Research Symposium (SURS)

Antibiotic-resistant bacteria are becoming more prevalent in soil as humans have increased impacts on the environment. Previous research proposed that earthworms could affect bacteria distribution as soil is ingested and filtered through their bodies. This research aims to see if kanamycin-resistance bacteria is the same in earthworm digestive tracts as in the soil the earthworms inhabit. We expect that the gut contents and soil will vary in the occurrence of kanamycin-resistant bacteria. Earthworms and composted soil samples were collected from compost bins at a residential home in Nashville, Tennessee. Digestive tracts were dissected from the earthworms and homogenized. Kanamycin-resistant bacteria ...


Investigating The Pi3k/Akt/Atm Pathway, Telomeric Dna Damage, T Cell Death, And Crispr/Cas9-Mediated Gene Editing During Acute And Chronic Hiv Infection, Sushant Khanal 2022 East Tennessee State University

Investigating The Pi3k/Akt/Atm Pathway, Telomeric Dna Damage, T Cell Death, And Crispr/Cas9-Mediated Gene Editing During Acute And Chronic Hiv Infection, Sushant Khanal

Electronic Theses and Dissertations

Human Immunodeficiency Virus (HIV) infection initiates major metabolic and cell- survival complications. Anti-retroviral therapy (ART) is the current approach to suppress active HIV replication to a level of undetected viral load, but it is not a curative approach. Newer and sophisticated gene editing technologies could indeed be a potent antiviral therapy to achieve a clinical sterilization/cure of HIV infection. Chronic HIV patients, even under a successful ART regimen, exhibit a low-grade inflammation, immune senescence, premature aging, telomeric DNA attrition, T cell apoptosis, and cellular homeostasis. In this dissertation, we investigated CD4 T cell homeostasis, degree of T cell apoptosis ...


Algae And Cyanobacteria In The Aphotic Habitats Of Veternica Cave (Medvednica Mt., Croatia) And Selected Caves Of The Dinaric Karst (South-Eastern Europe), Najla Baković, Tanja Pipan, Robert Baković, Roman Ozimec, Josip Rubinić, Renata Matoničkin Kepčija 2022 ADIPA – Society for Research and Conservation of Croatian Natural Diversity, Croatia

Algae And Cyanobacteria In The Aphotic Habitats Of Veternica Cave (Medvednica Mt., Croatia) And Selected Caves Of The Dinaric Karst (South-Eastern Europe), Najla Baković, Tanja Pipan, Robert Baković, Roman Ozimec, Josip Rubinić, Renata Matoničkin Kepčija

International Journal of Speleology

Microphototrophs (algae and cyanobacteria) in karst environments have been intensively studied in aquatic epigean habitats. In recent decades knowledge about the communities inhabiting cave entrances and lampenflora has grown substantially, but the data about the communities in aphotic cave zone are scarce. This study aimed to investigate spatio-temporal presence of microphototrophs in the aphotic zone of Veternica Cave (Mt. Medvednica karst) and to present additional preliminary data from 22 caves of the Dinaric karst. The data were collected over ten years, in parallel with research on cave phagotrophic protists. In addition to the remains of microphototrophs, living algae and cyanobacteria ...


Potential Use Of Proteolytic Bacteria Paenibacillus Dendritiformis (Bt7) Isolated From Batu Tannery Effluents For The Detergent Industry, Chandran Masi, Degafneh Tadesse, Abate Ayele 2022 Department of Biotechnology, College of Biological and Chemical Engineering, Addis Ababa Science and Technology University,

Potential Use Of Proteolytic Bacteria Paenibacillus Dendritiformis (Bt7) Isolated From Batu Tannery Effluents For The Detergent Industry, Chandran Masi, Degafneh Tadesse, Abate Ayele

Karbala International Journal of Modern Science

This research was aimed at identifying a bacterium that can produce alkaline proteases. As a result, bacteria that produce proteases were isolated from Batu tannery effluents, tested for protease synthesis on skim milk agar plates, and validated with a protease assay. Microscopic and molecular phylogenetic analyses identified Paenibacillus dendritiformis (BT7) as the bacterial isolate with the highest alkaline protease production. The isolate's maximum enzyme production was obtained by 2% inoculum size, 40°C temperature, 9.0 pH, and a 48-hour incubation time with production media components such as glucose, casein, MgCl2, and 2% NaCl. The maximal enzyme activity was ...


The Presence Of Microcystin In The Northwest Indiana Watershed, Breeann Mild 2022 Purdue University

The Presence Of Microcystin In The Northwest Indiana Watershed, Breeann Mild

The Journal of Purdue Undergraduate Research

No abstract provided.


College Of Natural Sciences Newsletter, September & October 2022, College of Natural Sciences 2022 South Dakota State University

College Of Natural Sciences Newsletter, September & October 2022, College Of Natural Sciences

College of Natural Sciences Newsletters and Reports

Volume 3, Issue 6

Page 1 Dean's Message
Page 2 Awards & Recognition
Page 3 Midwest Regional ACS Meeting
Page 4 North Central ASM Meeting
Page 5 Geography Department Travel
Page 6-7 Media Coverage of CNS
Page 7 REMAST Program at SDState receives national spotlight
Page 8 Celebrating the lives of those who touched the College
Page 9 Innovative Learning Spaces
Page 10 Open PRAIRIE Data
Page 11 2022 CNS Scholarship Brunch
Page 12-14 Fall 2022 Outreach Events


Occurrence Of Kanamycin-Resistant Bacteria Relative To Anthropogenic Pollution Along Richland Creek In Nashville, Tn​, Jolene Ho Mach, Annie Le, Brandon Torres Ramirez 2022 Belmont University

Occurrence Of Kanamycin-Resistant Bacteria Relative To Anthropogenic Pollution Along Richland Creek In Nashville, Tn​, Jolene Ho Mach, Annie Le, Brandon Torres Ramirez

Science University Research Symposium (SURS)

The overuse of antibiotics has caused an increase in antibiotic-resistant (AR) bacteria, which is a serious public health concern. Previous studies showed a significant correlation between anthropogenic pollution and AR bacteria. This project aims to identify AR bacteria in Richland Creek relative to local anthropogenic pollution. Water samples were collected at four locations along Richland Creek in Nashville, Tennessee. Bacteria resistant to the antibiotic kanamycin were isolated from the water samples, identified to genera using DNA barcoding, and compared among the sites. We expect to see a greater abundance and diversity of kanamycin-resistant bacteria closer to the end than near ...


Comparison Of The Humoral Immune Response Following Both Bacterial Challenge And Rnai Of Major Factors On Proliferation Of Bartonella Quintana In The Human Louse, Jake Zina 2022 University of Massachusetts Amherst

Comparison Of The Humoral Immune Response Following Both Bacterial Challenge And Rnai Of Major Factors On Proliferation Of Bartonella Quintana In The Human Louse, Jake Zina

Masters Theses

Human body lice, Pediculus humanus humanus, and head lice, Pediculus humanus capitis, have been hematophagous ectoparasites of humans for thousands of years. Despite being ecotypes, only body lice are known to transmit bacterial diseases to humans, and it appears that lower humoral and cellular immune responses allow body lice to possess a higher vector competence. We previously observed that the transcription level of the defensin 1 gene was up-regulated only in head lice following oral challenge of Bartonella quintana, a causative agent of trench fever, and also that body lice excreted more viable B. quintana in their feces. In this ...


The Sos Response In Escherichia Coli K12: An Exploration Of Mutations In Lexa And Reca Using Fluorescence Microscopy, Steven Van Alstine 2022 University of Massachusetts Amherst

The Sos Response In Escherichia Coli K12: An Exploration Of Mutations In Lexa And Reca Using Fluorescence Microscopy, Steven Van Alstine

Doctoral Dissertations

Faithful replication of the genome is paramount for maintaining the fitness of an organism. Therefore, life has evolved inducible mechanisms to be able to repair damaged DNA and maintain evolutionary fitness. The SOS response is a highly conserved DNA damage inducible response that is tightly regulated. Multiple factors contribute to the ability of the cell to perform proper DNA repair and induction of the SOS response including the amount of RecA, mutations in RecA that affect competition for DNA, and other proteins that interact with the RecA filament. The complex relationship between RecA and LexA is the subject of this ...


Principles Of Aaa+ Proteases, Samar Mahmoud 2022 University of Massachusetts Amherst

Principles Of Aaa+ Proteases, Samar Mahmoud

Doctoral Dissertations

ATPases associated with diverse cellular activities (AAA+) proteases in bacteria help maintain protein homeostasis by degrading misfolded and regulatory proteins. While a handful of protein targets for these proteases have been identified in Caulobacter crescentus and other organisms, more research is needed to elucidate mechanisms that govern substrate specificity. In the second chapter of this thesis, I will elaborate on how AAA+ substrate specificity is less rigid than previous work has suggested and how limiting ATP or mutations can alter substrate preferences of the ClpXP protease. In the third chapter, I will highlight our efforts to use a quantitative proteomics ...


Biomedical Applications Of Protein Films And Polymeric Nanomaterials, Sanjana Gopalakrishnan 2022 University of Massachusetts Amherst

Biomedical Applications Of Protein Films And Polymeric Nanomaterials, Sanjana Gopalakrishnan

Doctoral Dissertations

Biomaterials are widely applied for the diagnosis and treatment of numerous diseases. In addition to fulfilling specific biological functions, biomaterials must also be non-toxic, biocompatible, and sterilizable to be regarded as safe-for-use. Polymers are excellent candidates for fabricating functional biomaterials due to their wide availability and varied properties and may be natural or synthetic. Polymer precursors are fabricated into coatings, foams, scaffolds, gels, composites, and nanomaterials for several biomedical applications. This dissertation focuses on two types of polymeric biomaterials – protein-based materials and synthetic polymeric nanoparticles. Proteins are biopolymers that naturally occur with a variety of structural and functional properties. However ...


Viruses In Astrobiology, Ignacio de la Higuera, Ester Lázaro 2022 Portland State University

Viruses In Astrobiology, Ignacio De La Higuera, Ester Lázaro

Biology Faculty Publications and Presentations

Viruses are the most abundant biological entities on Earth, and yet, they have not received enough consideration in astrobiology. Viruses are also extraordinarily diverse, which is evident in the types of relationships they establish with their host, their strategies to store and replicate their genetic information and the enormous diversity of genes they contain. A viral population, especially if it corresponds to a virus with an RNA genome, can contain an array of sequence variants that greatly exceeds what is present in most cell populations. The fact that viruses always need cellular resources to multiply means that they establish very ...


Sars-Cov-2 Omicron (B.1.1.529) Variant: Structural Features, Biological Characteristics, Impact On Scientific Research, General Precautions And Protective Procedures; A Systematic Review, Prithivi Nagarajan, Anitha Vetrivel, Jayanthi Kumar, Anusheela Howlader, Kumar Rangarajalu, Satheesh Kumar Sabapathy, Muthu Gopal, Saravanaavel Kumar 2022 ASSISTANT PROFESSOR, MULTI-DISCIPLINARY CENTRE FOR BIOMEDICAL RESEARCH, AARUPADAI VEEDU MEDICAL COLLEGE & HOSPITAL, INDIA

Sars-Cov-2 Omicron (B.1.1.529) Variant: Structural Features, Biological Characteristics, Impact On Scientific Research, General Precautions And Protective Procedures; A Systematic Review, Prithivi Nagarajan, Anitha Vetrivel, Jayanthi Kumar, Anusheela Howlader, Kumar Rangarajalu, Satheesh Kumar Sabapathy, Muthu Gopal, Saravanaavel Kumar

Journal of Mind and Medical Sciences

The SARS-CoV-2 mutations that have occurred have alarmed the entire medical community. Thus, global concerns have been expressed regarding the transmission, pathogenicity and immune evasion of the Omicron strain, which shares mutations with the variants of concern Alpha, Beta, and Gamma strains. Still, Omicron transmission has crossed numerous borders worldwide compared to other types of SARS-CoV-2. The number of confirmed cases has increased and is reappearing in India. Given the worrisome situation created by the Omicron outbreak, scientists and postgraduates have had to make decisions about current research programs at institutions around the globe. The Omicron variants under development have ...


Identification And Antibiotic-Susceptibility Profiling Of Non-Hospital Wound Infecting Staphylococcus Aureus Isolated From Ghail-Bawazeer Patients, Yemen, Maged Salem Bin-Masalam, A. Ba-Swaqi, A. Hwydi, A. Mhedy, E. Alamudi, H. Bin-Nashe, M. Abozid, S. Aljomhy, S. Badaood 2022 Hadhramout University

Identification And Antibiotic-Susceptibility Profiling Of Non-Hospital Wound Infecting Staphylococcus Aureus Isolated From Ghail-Bawazeer Patients, Yemen, Maged Salem Bin-Masalam, A. Ba-Swaqi, A. Hwydi, A. Mhedy, E. Alamudi, H. Bin-Nashe, M. Abozid, S. Aljomhy, S. Badaood

Hadhramout University Journal of Natural & Applied Sciences

The continuous increase in the resistance of pathogenic Staphylococcus aureus isolated from non-hospital infected wounds to the novel chemical classes of antibiotics is of great public health importance. This problem prompted the researchers to study the antibiotic susceptibility of such strains. Twenty wound pus samples were collected and selected randomly from 20 non-hospital wound infected patients of different ages who attended different medical laboratories in Ghail-Bawazeer, Yemen, from October to December 2020, and 24 bacterial isolates were analyzed using morphological, cultural, and biochemical characterization for the isolation and identification of S. aureus. Based on the morphological and cultural characteristics, only ...


Leaf Phenology And Freeze Tolerance Of The Invasive Tree Pyrus Calleryana (Roseaceae) And Potential Native Competitors, Margaret E. Maloney, Abby Hay, Eric B. Borth, Ryan W. McEwan 2022 University of Dayton

Leaf Phenology And Freeze Tolerance Of The Invasive Tree Pyrus Calleryana (Roseaceae) And Potential Native Competitors, Margaret E. Maloney, Abby Hay, Eric B. Borth, Ryan W. Mcewan

Biology Faculty Publications

Pyrus calleryana is one of the most problematic invasive species in the eastern United States. The mechanisms that enable Py. calleryana to establish and outcompete native plants are not fully understood but likely include a profile of advantageous traits. Extended leaf phenology is a characteristic noted in many woody invasive plants. Leaf phenology of Py. calleryana and two native woody species, Populus deltoides and Platanus occidentalis, was observed in natural areas near Dayton, OH from December 2019 to November 2020. A frost event in May also gave us the serendipitous opportunity to assess frost tolerance of these species. We found ...


Development And Optimization Of An Immunoassay For The Detection Of Antibodies Against Sars-Cov-2 With In-House Recombinant Rbd Protein, Safira Pinaka Pramestika Ratu, Silmi Mariya, Rachmitasari Noviana, Uus Saepuloh, Huda Salahudin Darusman 2022 Biotechnology, Multidiciplinary Program, Institut Pertanian Bogor, Bogor 16680, Indonesia

Development And Optimization Of An Immunoassay For The Detection Of Antibodies Against Sars-Cov-2 With In-House Recombinant Rbd Protein, Safira Pinaka Pramestika Ratu, Silmi Mariya, Rachmitasari Noviana, Uus Saepuloh, Huda Salahudin Darusman

Makara Journal of Science

COVID-19 caused by SARS-CoV-2 poses a major threat to the global community, particularly in Indonesia. Countermeasures to prevent the spread of this disease have also been implemented, including the implementation of a vaccination program. An immunoassay technique that can be used to analyze antibodies that might develop following vaccination is the indirect enzyme-linked immunosorbent assay (ELISA). We produced the recombinant spike protein used in this study. The optimization comprised adjusted concentrations of spike recombinant protein (5 and 10 ng/mL), blocking agent (2.5% and 5%), and conjugate (1:1000 and 1:5000). The optimal conditions in this study included ...


Mutation Of Aspergillus Sp. Using Ultraviolet Light And Nitrous Acid For Amylase Production From Banana Peels, Cyprian Erumiseli Oshoma, Johnson Oche Akor, Beckley Ikhajiagbe, Marcel James Ikenebomeh 2022 Department of Microbiology, University of Benin, Benin City 300213, Nigeria

Mutation Of Aspergillus Sp. Using Ultraviolet Light And Nitrous Acid For Amylase Production From Banana Peels, Cyprian Erumiseli Oshoma, Johnson Oche Akor, Beckley Ikhajiagbe, Marcel James Ikenebomeh

Makara Journal of Science

Strain improvement through mutagenesis is important in the commercial development of microbial fermentation. This study aimed to produce amylase from banana (Musa sapientum) peels by using mutant Aspergillus sp. strains obtained via ultraviolet (UV) radiation and nitrous acid-based mutagenesis. Results revealed that banana peels pretreated with 0.8 N HCl had a biomass yield of 3.02 g/L and amylase activity of 2.81 U/L. Of the UV mutants, strain AUV1 had the highest biomass and amylase activity of 4.50 ± 0.21 g/L and 3.46 ± 0.14 U/L, respectively. Subsequently, HNO2 mutants showed ...


Ridinilazole: A Novel, Narrow-Spectrum Antimicrobial Agent Targeting Clostridium (Clostridioides) Difficile, Deirdre A. Collins, Thomas V. Riley 2022 Edith Cowan University

Ridinilazole: A Novel, Narrow-Spectrum Antimicrobial Agent Targeting Clostridium (Clostridioides) Difficile, Deirdre A. Collins, Thomas V. Riley

Research outputs 2022 to 2026

Clostridium (Clostridioides) difficile infection (CDI) remains an urgent threat to patients in health systems worldwide. Recurrent CDI occurs in up to 30% of cases due to sustained dysbiosis of the gut microbiota which normally protects against CDI. Associated costs of initial and recurrent episodes of CDI impose heavy financial burdens on health systems. Vancomycin and metronidazole have been the mainstay of therapy for CDI for many years; however, these agents continue to cause significant disruption to the gut microbiota and thus carry a high risk of recurrence for CDI patients. Treatment regimens are now turning towards novel narrow spectrum antimicrobial ...


The Cryptococcus Neoformans Flc1 Homologue Controls Calcium Homeostasis And Confers Fungal Pathogenicity In The Infected Hosts, Piotr R. Stempinski, Kristie D. Goughenour, Lukas M. du Plooy, J. Andrew Alspaugh, Michal A. Olszewski, Lukasz Kozubowski 2022 Clemson University

The Cryptococcus Neoformans Flc1 Homologue Controls Calcium Homeostasis And Confers Fungal Pathogenicity In The Infected Hosts, Piotr R. Stempinski, Kristie D. Goughenour, Lukas M. Du Plooy, J. Andrew Alspaugh, Michal A. Olszewski, Lukasz Kozubowski

Open Access Publishing Fund

Cryptococcus neoformans, an opportunistic yeast pathogen, relies on a complex network of stress response pathways that allow for proliferation in the host. In Saccharomyces cerevisiae, stress responses are regulated by integral membrane proteins containing a transient receptor potential (TRP) domain, including the flavin carrier protein 1 (Flc1), which regulates calcium homeostasis and flavin transport. Here, we report that deletion of C. neoformans FLC1 results in cytosolic calcium elevation and increased nuclear content of calcineurin-dependent transcription factor Crz1, which is associated with an aberrant cell wall chitin overaccumulation observed in the flc1Δ mutant. Absence of Flc1 or inhibition of calcineurin ...


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