Microbiome Of Commercial Broilers Through Evisceration And Immersion Chilling, 2018 University of Arkansas, Fayetteville
Microbiome Of Commercial Broilers Through Evisceration And Immersion Chilling, John A. Handley
Theses and Dissertations
The United States poultry industry generated 38.6 billion pounds (17,500 metric ton) of meat in 2014 which averaged to 121 pounds (55 kg) per individual of the U.S that same year. Of that meat generated by the poultry industry, an estimated 1 million cases of Salmonellosis will occur. Out of the 1 million cases approximately 40, 000 to 50,000 will be confirmed cases by the CDC. Recently, the USDA has requested changes in the inspection process and are currently allowing processors more freedom to utilize innovation to drive the increase in safer and more desirable foods ...
The Effect Of Coriander Extract On The Replication Of T2 Bacteriophage, 2018 Ohio Wesleyan University
The Effect Of Coriander Extract On The Replication Of T2 Bacteriophage, Marielle Buss
Coriander, also known as cilantro or Chinese parsley, is an annual herb in the family Apiaceae. All parts of the plant are edible, but the fresh leaves and the dried seeds are the parts most traditionally used in cooking. Coriander has been shown to have antibacterial and antiviral activity for viruses of humans in vitro. Increasing concentrations of coriander seed extract added to Luria-Bertani broth have no effect on the growth of E. coli. The addition of E. coli coriander seed extract to Luria-Bertani broth at a concentration of 0.00012 g/ml enhances the yield of T2 bacteriophage up ...
Glyphosate Inhibits Keratinolytic Activity Of Bacillus Spp. Isolated From Wild Songbirds, 2018 Ohio Wesleyan University
Glyphosate Inhibits Keratinolytic Activity Of Bacillus Spp. Isolated From Wild Songbirds, Elizabeth Urbanski
Glyphosate is a frequently-used herbicide both domestically and in the agricultural industry worldwide. While the effect of glyphosate ingestion on bird health has been studied, less is known about the potential impact of glyphosate on bacteria found on avian plumage. This study investigated the effect of glyphosate on the degradation of bird feathers by keratinolytic plumage bacteria. To determine the effect of glyphosate on bacterial feather degradation, two strains of Bacillus spp. isolated from the plumage of wild songbirds captured in mist nets were tested. A disk-diffusion assay was used to determine that 8.44 g/L of glyphosate inhibited ...
The Effects Of Inulin And Galactooligosaccharides On The Production Of Reuterin By Lactobacillus Reuteri, 2018 Olivet Nazarene University
The Effects Of Inulin And Galactooligosaccharides On The Production Of Reuterin By Lactobacillus Reuteri, Micah Forshee
Scholar Week 2016 - present
The microbiome is a dynamic community that can positively and negatively influence host health. Lactobacillus reuteri is a probiotic that has received much attention for its ability to inhibit pathogens such as Salmonella Typhimurium, Escherichia coli, and Clostridium difficile. It does so by its unique ability to metabolize glycerol into the antimicrobial compound 3-HPA, which is commonly referred to as reuterin. The ability to secrete reuterin is dependent not only on glycerol availability but also the concentration of glucose. In fact, there appears to be a “goldilocks” ratio between glucose and glycerol as either too much or too little glucose ...
The Subject Librarian Newsletter, Biology, Fall 2016, 2018 University of Central Florida
The Subject Librarian Newsletter, Biology, Fall 2016, Sandy Avila
No abstract provided.
The Subject Librarian Newsletter, Biology, Spring 2017, 2018 University of California, Irvine School of Law
The Subject Librarian Newsletter, Biology, Spring 2017, Sandy Avila
No abstract provided.
Microbiology (Sgsc), 2018 South Georgia State College
Microbiology (Sgsc), Molly Smith, Sara Selby
Biological Sciences Grants Collections
This Grants Collection for Microbiology was created under a Round Six ALG Textbook Transformation Grant.
Affordable Learning Georgia Grants Collections are intended to provide faculty with the frameworks to quickly implement or revise the same materials as a Textbook Transformation Grants team, along with the aims and lessons learned from project teams during the implementation process.
Documents are in .pdf format, with a separate .docx (Word) version available for download. Each collection contains the following materials:
- Linked Syllabus
- Initial Proposal
- Final Report
A Cultured Learning Environment: Implementing A Problem- And Service-Based Microbiology Capstone Course To Assess Process- And Skill-Based Learning Objectives, Rachel M. Watson, John D. Willford, Mariel A. Pfeifer
Interdisciplinary Journal of Problem-Based Learning
In this study, a problem-based capstone course was designed to assess the University of Wyoming Microbiology Program’s skill-based and process-based student learning objectives. Students partnered with a local farm, a community garden, and a free downtown clinic in order to conceptualize, propose, perform, and present studies addressing problems experienced by these partners. Instructor assessments enabled understanding of student competencies, and according to external subject matter experts students demonstrated mastery of all learning objectives on the final research presentation. Community partners were completely satisfied with the students’ solutions, professionalism, and communication. Instructional diagnosis and student course evaluations showed satisfaction, engagement ...
Integrating Tuberculosis And Antimicrobial Resistance Control Programmes, 2018 Aga Khan University
Integrating Tuberculosis And Antimicrobial Resistance Control Programmes, Rumina Hasan, Sadia Shakoor, Johanna Hanefeld, Mishal Khan
Department of Pathology and Laboratory Medicine
Many low- and middle-income countries facing high levels of antimicrobial resistance, and the associated morbidity from ineffective treatment, also have a high burden of tuberculosis. Over recent decades many countries have developed effective laboratory and information systems for tuberculosis control. In this paper we describe how existing tuberculosis laboratory systems can be expanded to accommodate antimicrobial resistance functions. We show how such expansion in services may benefit tuberculosis case-finding and laboratory capacity through integration of laboratory services. We further summarize the synergies between high-level strategies on tuberculosis and antimicrobial resistance control. These provide a potential platform for the integration of ...
Gas Plasma Pre-Treatment Increases Antibiotic Sensitivity And Persister Eradication In Methicillin-Resistant Staphylococcus Aureus, Li Guo, Ruobing Xu, Yiming Zhao, Dingxin Liu, Zhijie Liu, Xiaohua Wang, Hailan Chen, Michael G. Kong
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of serious nosocomial infections, and recurrent MRSA infections primarily result from the survival of persister cells after antibiotic treatment. Gas plasma, a novel source of ROS (reactive oxygen species) and RNS (reactive nitrogen species) generation, not only inactivates pathogenic microbes but also restore the sensitivity of MRSA to antibiotics. This study further found that sublethal treatment of MRSA with both plasma and plasma-activated saline increased the antibiotic sensitivity and promoted the eradication of persister cells by tetracycline, gentamycin, clindamycin, chloramphenicol, ciprofloxacin, rifampicin, and vancomycin. The short-lived ROS and RNS generated by plasma ...
Emergence Of An Extensively Drug-Resistant Salmonella Enterica Serovar Typhi Clone Harboring A Promiscuous Plasmid Encoding Resistance To Fluoroquinolones And Third-Generation Cephalosporins, 2018 Agha Khan University
Emergence Of An Extensively Drug-Resistant Salmonella Enterica Serovar Typhi Clone Harboring A Promiscuous Plasmid Encoding Resistance To Fluoroquinolones And Third-Generation Cephalosporins, Rumina Hasan, Sadia Shakoor, Farah Naz Farid, Dania Khalid Saeed, Ghazala Shaheen, Shahida Qureshi, Mohammad Tahir Yousafzai, Muhammad Khalid Salim, Zahra Hasan, Elizabeth J. Klemma, Andrew J. Page, Kim Judge, Vanessa K. Wong, Timothy J. Dallman, Satheesh Nair, Stephen Baker, Gordon Dougan
Department of Pathology and Laboratory Medicine
Antibiotic resistance is a major problem in Salmonella enterica serovar Typhi, the causative agent of typhoid. Multidrug-resistant (MDR) isolates are prevalent in parts of Asia and Africa and are often associated with the dominant H58 haplotype. Reduced susceptibility to fluoroquinolones is also widespread, and sporadic cases of resistance to third-generation cephalosporins or azithromycin have also been reported. Here, we report the first large-scale emergence and spread of a novel S. Typhi clone harboring resistance to three first-line drugs (chloramphenicol, ampicillin, and trimethoprim-sulfamethoxazole) as well as fluoroquinolones and third-generation cephalosporins in Sindh, Pakistan, which we classify as extensively drug resistant (XDR ...
Functional And Structural Mimicry Of A-Kinase Anchoring Proteins By Human Adenovirus E1a, 2018 The University of Western Ontario
Functional And Structural Mimicry Of A-Kinase Anchoring Proteins By Human Adenovirus E1a, Cason R. King
Electronic Thesis and Dissertation Repository
As an obligate intracellular parasite, human adenovirus (HAdV) must utilize host factors for survival and replication. Early during infection, its multifunctional E1A protein interacts with an impressive range of cellular target proteins to exert control over the cellular environment. Through these virus-host interactions, E1A massively reprograms both viral and cellular transcription to activate the other HAdV genes, downregulate the host’s immune response, and induce the cell cycle. Consequently, E1A converts the infected cell into a compliant state more amenable for HAdV replication, resulting from its numerous protein-protein interactions. I sought to examine E1A’s interaction with cellular protein kinase ...
Lysine Biosynthesis In Bacteria: A Metallodesuccinylase As A Potential Antimicrobial Target, 2018 Silesian University of Technology
Lysine Biosynthesis In Bacteria: A Metallodesuccinylase As A Potential Antimicrobial Target, Danuta M. Gillner, Daniel P. Becker Ph.D., Richard C. Holz
Richard C. Holz
In this review, we summarize the recent literature on dapE-encoded N-succinyl-l,l-diaminopimelic acid desuccinylase (DapE) enzymes, with an emphasis on structure–function studies that provide insight into the catalytic mechanism. Crystallographic data have also provided insight into residues that might be involved in substrate and hence inhibitor recognition and binding. These data have led to the design and synthesis of several new DapE inhibitors, which are described along with what is known about how inhibitors interact with the active site of DapE enzymes, including the efficacy of a moderately strong DapE inhibitor.
Sodium Polyphosphate And Polyethylenimine Enhance The Antimicrobial Activities Of Plant Essential Oils, 2018 Iowa State University
Sodium Polyphosphate And Polyethylenimine Enhance The Antimicrobial Activities Of Plant Essential Oils, Heidi A. Wright, Byron F. Brehm-Stecher
Byron F. Brehm-Stecher
Plant extracts have been used for millennia for treatment of disease, with much recent interest focusing on the antimicrobial activities of plant essential oils (EOs). Although EOs are active against common microbial pathogens, their effective use as topical, environmental, or food antimicrobials will require EO-based formulations with enhanced antimicrobial activities. In this study, two polyionic compounds, sodium polyphosphate (polyP, a polyanion) and polyethylenimine (PEI, a polycation), were evaluated for their abilities to enhance the antimicrobial activities of six EOs against the human pathogens Escherichia coli O157:H7, Salmonella enterica subsp. enterica ser. Minnesota, Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus aureus, and ...
Water Supply In Developing Countries, 2018 Purdue University
Water Supply In Developing Countries, Jason K. Hawes, Ernest R. Blatchley Iii, Audrey Caprio, Andrew T. Kanach
Engagement & Service-Learning Summit: Reciprocal & Sustainable Partnerships
No abstract provided.
Is There A Link Between Aging And Microbiome Diversity In Exceptional Mammalian Longevity?, 2018 University College Dublin - Ireland
Is There A Link Between Aging And Microbiome Diversity In Exceptional Mammalian Longevity?, Graham M. Hughes, John Leech, Sebastien J. Puechmaille, Jose V. Lopez, Emma C. Teeling
Biology Faculty Articles
A changing microbiome has been linked to biological aging in mice and humans, suggesting a possible role of gut flora in pathogenic aging phenotypes. Many bat species have exceptional longevity given their body size and some can live up to ten times longer than expected with little signs of aging. This study explores the anal microbiome of the exceptionally long-lived Myotis myotis bat, investigating bacterial composition in both adult and juvenile bats to determine if the microbiome changes with age in a wild, long-lived non-model organism, using non-lethal sampling. The anal microbiome was sequenced using metabarcoding in more than 50 ...
Stress-Induced Reorganization Of The Mycobacterial Membrane Domain, 2018 University of Massachusetts Amherst
Stress-Induced Reorganization Of The Mycobacterial Membrane Domain, Jennifer M. Hayashi, Kirill Richardson, Emily S. Melzer, Steven J. Sandler, Bree B. Aldridge, M. Sloan Siegrist, Yasu S. Morita
Microbiology Department Faculty Publication Series
Cell elongation occurs primarily at the mycobacterial cell poles, but the molecular mechanisms governing this spatial regulation remain elusive. We recently reported the presence of an intracellular membrane domain (IMD) that was spatially segregated from the conventional plasma membrane in Mycobacterium smegmatis. The IMD is enriched in the polar region of actively elongating cells and houses many essential enzymes involved in envelope biosynthesis, suggesting its role in spatially restricted elongation at the cell poles. Here, we examined reorganization of the IMD when the cells are no longer elongating. To monitor the IMD, we used a previously established reporter strain expressing ...
Genome Sequence Of Verrucomicrobium Sp. Strain Gas474, A Novel Bacterium Isolated From Soil, 2018 University of Massachusetts Amherst
Genome Sequence Of Verrucomicrobium Sp. Strain Gas474, A Novel Bacterium Isolated From Soil, Grace Pold, Erin M. Conlon, Marcel Huntemann, Manoj Pillay, Natalia Mikhailova, Dimitrios Stamatis, T.B.K. Reddy, Chris Daum, Nicole Shapiro, Nikos Kyrpides, Tanja Woyke, Kristen Deangelis
Microbiology Department Faculty Publication Series
Verrucomicrobium sp. strain GAS474 was isolated from the mineral soil of a temperate deciduous forest in central Massachusetts. Here, we present the complete genome sequence of this phylogenetically novel organism, which consists of a total of 3,763,444 bp on a single scaffold, with a 65.8% GC content and 3,273 predicted open reading frames.
Draft Genome Sequence Of Streptomyces Sp. Strain Jv178, A Producer Of Clifednamide-Type Polycyclic Tetramate Macrolactams, 2018 Washington University in St. Louis
Draft Genome Sequence Of Streptomyces Sp. Strain Jv178, A Producer Of Clifednamide-Type Polycyclic Tetramate Macrolactams, Yunci Qi, John M. D’Alessandro, Joshua A.V Blodgett
Biology Faculty Publications & Presentations
Here, we report the draft genome sequence of Streptomyces sp. JV178, a strain originating from Connecticut (USA) garden soil. This strain produces the polycyclic tetramate macrolactam compounds clifednamides A and B. The draft genome contains 10.65 Mb, 9,045 predicted protein coding sequences, and several natural product biosynthetic loci.
Mrub_2120, Mrub_2121, Mrub_2122, Mrub_2123 And Mrub_2124 Are Orthologs Of E. Coli Genes B3458, B3457, B3456, B3455 And B3454, Respectively, And Make Up An Operon That Codes For The Branched-Chain Amino Acid Abc Transporter In Meiothermus Ruber Dsm 1279, 2018 Augustana College, Rock Island Illinois
Mrub_2120, Mrub_2121, Mrub_2122, Mrub_2123 And Mrub_2124 Are Orthologs Of E. Coli Genes B3458, B3457, B3456, B3455 And B3454, Respectively, And Make Up An Operon That Codes For The Branched-Chain Amino Acid Abc Transporter In Meiothermus Ruber Dsm 1279, Aaron Jones, Madelyn Huber, Dr. Lori Scott
Meiothermus ruber Genome Analysis Project
In this project we investigated the biological function of the genes Mrub_2120, Mrub_2121, Mrub_2122, Mrub_2123 and Mrub_2124 (KEGG map number 02010). We predict these genes encode components of a branched-chain amino acid ATP Binding Cassette (ABC) transporter: 1) Mrub_2120 (DNA coordinates 2169247-2170416 on the reverse strand) encodes the branched-chain amino acid binding protein that is localized to the periplasm; 2) Mrub_2121 (DNA coordinates 2170433..2171353 on the reverse strand) encodes the first TMD; 3) Mrub_2122 (DNA coordinates 2171365..2172279 on the reverse strand) encodes the second TMD; 4) Mrub_2123 (DNA coordinates 2172276..2173028 on the reverse strand) encodes the first ...