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Investigation Of Competence Heterogeneity In Streptococcus Milleri Group Clinical Isolates, Anne-Marie G. Lacroix 2014 McMaster University

Investigation Of Competence Heterogeneity In Streptococcus Milleri Group Clinical Isolates, Anne-Marie G. Lacroix

Open Access Dissertations and Theses

The Streptococcus Milleri/Anginosus group (SMG) includes Streptococcus anginosus, Streptococcus constellatus and Streptococcus intermedius. The SMG is found in healthy individuals but these bacteria are most known clinically for being associated with invasive disease and more recently, airway infections including cystic fibrosis (CF). The SMG like many other streptococci are naturally competent, being able to actively bind, uptake and integrate extracellular DNA. Competence regulation involves a competence-stimulating peptide (CSP) derived from the ComC precursor and a two- component signaling system (a histidine kinase ComD and its response regulator ComE). In this study, I examined the distribution of CSP/ComD sequences ...


From Progesterone In Biopsies To Estimates Of Pregnancy Rates: Large Scale Reproductive Patterns Of Two Sympatric Species Of Common Dolphin, Delphinus Spp. Off California, Usa And Baja, Mexico, Nicholas Marc Kellar, Marisa L. Trego, Susan J. Chivers, Fredrick I. Archer, Wayne L. Perryman 2014 Occidental College

From Progesterone In Biopsies To Estimates Of Pregnancy Rates: Large Scale Reproductive Patterns Of Two Sympatric Species Of Common Dolphin, Delphinus Spp. Off California, Usa And Baja, Mexico, Nicholas Marc Kellar, Marisa L. Trego, Susan J. Chivers, Fredrick I. Archer, Wayne L. Perryman

Bulletin of the Southern California Academy of Sciences

Blubber progesterone levels were measured in biopsy samples and used to predict the pregnancy status of 507 female common dolphins (204 long-beaked common dolphins, Delphinus capensis, and 303 short-beaked common dolphins, D. delphis). Samples were collected in the coastal waters of the eastern North Pacific between central California, USA and the southern end of Baja California, Mexico. The percentage of females pregnant was similar between the two species: 22.1% (n = 45) of D. capensis and 28.1% (n = 85) of D. delphis. For both species we found strong geographic patterns in pregnancy, suggesting that some areas were more conducive ...


Permaculture Design: On The Practice Of Radical Imagination, Katja Rothe 2014 University of Massachusetts - Amherst

Permaculture Design: On The Practice Of Radical Imagination, Katja Rothe

communication +1

Permaculture design is a concept that aims at transforming not only agriculture, but also city planning, architecture, development, etc. In short it aims to change human habitats. It is part of a new ecological paradigm that is currently spreading in popularity from the urban gardening movement to various other alternative movements such as the slow movement, sustainable architecture, etc. Permaculture design defines itself as building on systems theory (as formulated in particular by Howard Thomas Odum and Christopher Alexander). However I would like to propose that the afterlife of systems theory as expressed in the concept of permaculture, first developed ...


Fermented Food: Putting The Power Of Good Health Back Into The Hands Of The People, Marsha L. Thadison 2014 Tuskegee University

Fermented Food: Putting The Power Of Good Health Back Into The Hands Of The People, Marsha L. Thadison

Professional Agricultural Workers Journal

Editors’ Note: At the December 2013 Professional Agricultural Workers Conference, several women were invited to share, information about their unique businesses. One such presenter was Marsha L. Thadison, owner of Yesterday’s Kitchen 4 Today, a company that focuses on promoting functional foods. Thadison shared that food is produce and consumed differently from previous generations. Today's food, she argued, involves many more agricultural inputs that aid in food preservation, extension of shelf life, and promotion of animal health. She espoused the benefits of fermented food.


The Innate Immune Response In Eisenia Fetida To Microbial Challenges, Aaron Schindler 2014 Minnesota State University, Mankato

The Innate Immune Response In Eisenia Fetida To Microbial Challenges, Aaron Schindler

Journal of Undergraduate Research at Minnesota State University, Mankato

The common earthworm, Eisenia fetida, exhibits a rudimentary immune system. The earthworm needs cellular and chemical responses against a constant microbial exposure from its natural environment. Some cellular and chemical responses are found in the coelomic fluid and have been shown to demonstrate anti-microbial characteristics. This project uses microscopy and modified staining techniques to differentiate and categorize the cellular components found in the coelomic fluid. Following a microbial challenge by Klebsiella pneumoniae, an inflammatory response was initiated. Six groups of earthworms were injected with 0.05 ml of 1.0 x 106 cfu /ml K. pneumoniae on day one and ...


Transcriptomic Analysis Of Clostridium Thermocellum Populus Hydrolysate-Tolerant Mutant Strain Shows Increased Cellular Efficiency In Response To Populus Hydrolysate Compared To The Wild Type Strain, Jessica L. Linville, Miguel Rodriguez, Steve D. Brown, Jonathan R. Mielenz, Chris D. Cox 2014 University of Tennessee, Knoxville

Transcriptomic Analysis Of Clostridium Thermocellum Populus Hydrolysate-Tolerant Mutant Strain Shows Increased Cellular Efficiency In Response To Populus Hydrolysate Compared To The Wild Type Strain, Jessica L. Linville, Miguel Rodriguez, Steve D. Brown, Jonathan R. Mielenz, Chris D. Cox

Microbiology Publications and Other Works

Background: The thermophilic, anaerobic bacterium, Clostridium thermocellum is a model organism for consolidated processing due to its efficient fermentation of cellulose. Constituents of dilute acid pretreatment hydrolysate are known to inhibit C. thermocellum and other microorganisms. To evaluate the biological impact of this type of hydrolysate, a transcriptomic analysis of growth in hydrolysate-containing medium was conducted on 17.5%?v/v Populus hydrolysate-tolerant mutant (PM) and wild type (WT) strains of C. thermocellum.ResultsIn two levels of Populus hydrolysate medium (0% and 10%?v/v), the PM showed both gene specific increases and decreases of gene expression compared to the ...


Diversity Of Bacteria Carried By Pinewood Nematode In Usa And Phylogenetic Comparison With Isolates From Other Countries, Diogo Neves Proença, Luís Fonseca, Thomas Powers, Isabel M.O. Abrantes, Paula V. Morais 2014 University of Nebraska - Lincoln

Diversity Of Bacteria Carried By Pinewood Nematode In Usa And Phylogenetic Comparison With Isolates From Other Countries, Diogo Neves Proença, Luís Fonseca, Thomas Powers, Isabel M.O. Abrantes, Paula V. Morais

Papers in Plant Pathology

Pine wilt disease (PWD) is native to North America and has spread to Asia and Europe. Lately, mutualistic relationship has been suggested between the pinewood nematode (PWN), Bursaphelenchus xylophilus the causal nematode agent of PWD, and bacteria. In countries where PWN occurs, nematodes from diseased trees were reported to carry bacteria from several genera. However no data exists for the United States. The objective of this study was to evaluate the diversity of the bacterial community carried by B. xylophilus, isolated from different Pinus spp. with PWD in Nebraska, United States. The bacteria carried by PWN belonged to Gammaproteobacteria (79 ...


Characterization Of Protein-Protein Interactions Mediated By The Second Exon Of The Varicella-Zoster Virus Orf45/42 Terminase Subunit, Elizabeth A. Syster-Clevenger 2014 Indiana University – Purdue University Fort Wayne

Characterization Of Protein-Protein Interactions Mediated By The Second Exon Of The Varicella-Zoster Virus Orf45/42 Terminase Subunit, Elizabeth A. Syster-Clevenger

Master's Theses

Varicella-zoster virus (VZV), a member of the Herpesviridae, is ubiquitous human pathogen with significant morbidity and mortality in healthy and immunocompromised individuals. Primary infection causes a systemic viremia and skin rash known as varicella, and more commonly referred to as chickenpox. Latency is established in the nervous system during primary infection and can reactivate to cause zoster, more commonly known as shingles. Current treatments for VZV infection target viral DNA replication, primarily the DNA polymerase. However, novel small molecule inhibitors that target other viral replication processes, for example viral DNA encapsidation, offer additional therapeutic options. Seven conserved open reading frames ...


Growing Conditions For Algae, Angeles Mora, Tamar Melkonian, Alejandro Calderon-Urrea 2014 California Polytechnic State University

Growing Conditions For Algae, Angeles Mora, Tamar Melkonian, Alejandro Calderon-Urrea

STEM Teacher and Researcher (STAR) Program Posters

With the growing demands of water in California and the increasing cost of fossil fuels to operate water-cleaning equipment, Algae was cultivated in different concentrations of Nitrogen, Phosphorus, and Potassium (NPK) during different trails to identify the best growing conditions for the removal water contaminates. Before testing algae in waste water, the Algae: Dunaliella Primolecta, Chlorella Vulgaris, and Scenedesmus Dimorphus were cultivated in four different media containing distilled water and plant vitamins. The different mediums used include: Orchid Grow More (20-20-20), Miracle Grow (30-10-10), Murashige & Skoog, and Orchid Bloom Boster (11-35-15). As a result of the investigation, the algae, Scenedesmus ...


Phylogenetic Diversity Of Microbial Isolates From The Mars Pathfinder, Kyla BradyLong, Adriana Blachowicz, Parag Vaishampayan, James N. Benardini, Wayne Schubert 2014 California Polytechnic State University

Phylogenetic Diversity Of Microbial Isolates From The Mars Pathfinder, Kyla Bradylong, Adriana Blachowicz, Parag Vaishampayan, James N. Benardini, Wayne Schubert

STEM Teacher and Researcher (STAR) Program Posters

As spacecraft are sent to different planets, they take with them microscopic pieces of life from Earth. It is the task of the Biotechnology and Planetary Protection Group to keep as much of this life off other planets as possible as well as document any life that may have been sent. During the construction of the Mars Pathfinder, samples were collected from various locations on the spacecraft to test for contamination. These samples were then isolated, grown, documented, preserved and their 16S rRNA genes were sequenced for identification. The 16S rRNA gene sequence is utilized because it is a highly ...


Detection Of Microbial Catecholamines In A Model Wound Environment, Ryan F. Wasurick 2014 California Polytechnic State University

Detection Of Microbial Catecholamines In A Model Wound Environment, Ryan F. Wasurick

STEM Teacher and Researcher (STAR) Program Posters

Chronic wounds are defined as an injury that does not heal in a predictable pattern or time frame. The healing process is composed of specific stages and chronic wounds have been interrupted in one or more stages of this process. This project focuses on the bacterial component of an infected wound, specifically on the catecholamines influencing the bacteria in the wound site. The assay developed in this experiment is designed to optimally grow bacteria in a simulated eukaryotic wound environment, and evaluate biofilm expression and catecholamine production of the bacteria. Bacteria used in this study are Staphylococcus aureus and Pseudomonas ...


Ice Caves On Extraterrestrial Bodies: What Are The Prospects For Speleogenesis And Detection?, Penelope J. Boston 2014 University of South Florida

Ice Caves On Extraterrestrial Bodies: What Are The Prospects For Speleogenesis And Detection?, Penelope J. Boston

The International Workshop on Ice Caves

Potential mechanisms for creating cavities in icy extraterrestrial bodies have been tentatively explored by several authors. On one hand we have examples of mechanisms that create caves in water ice here on Earth. In addition, there may be unique mechanisms on other Solar System objects that do not occur on Earth but might produce cavities, e.g. sublimation of comets upon perihelion passage. The methods of detecting such cavities depend upon the nature of the icy body in question, the potential for orbital or landed missions to visit those bodies in the future, and remote or landed methods for detecting ...


Comparison Of The Promega And Kikkoman Atp Devices, Ryan Hendrickson, James Nick Benardini, Kasthuri Venkateswaran 2014 California Polytechnic State University

Comparison Of The Promega And Kikkoman Atp Devices, Ryan Hendrickson, James Nick Benardini, Kasthuri Venkateswaran

STAR (STEM Teacher and Researcher) Program Posters

Planetary Protection (PP) is a scientific discipline that aims to protect other celestial bodies from microorganisms found on Earth by preventing forward and backward contamination. Forward contamination is bringing life from Earth to a given celestial body and backward contamination is preventing contamination of eventual sample return missions from Mars back to Earth. PP verifies that NASA Planetary Protection requirements are met in part via the NASA Standard Assay in order to support the launch approval process and to comply with international law. The NASA Standard Assay verifies these bioburden requirements by quantifying any aerobic, heterotrophic, and heat-shock resistant isolates ...


Potential Roles Of Peroxidases In Caenorhabditis Elegans Innate Immunity, George R. Tiller, george r. tiller 2014 Texas Medical Center Library

Potential Roles Of Peroxidases In Caenorhabditis Elegans Innate Immunity, George R. Tiller, George R. Tiller

UT GSBS Dissertations and Theses (Open Access)

The production of ROS (reactive oxygen species) in response to pathogen detection is a rapid, nonspecific response that is evolutionarily conserved from nematodes to humans. ROS serve as direct and indirect effectors of innate and adaptive immunity. In Caenorhabditis elegans, a ROS burst is observed during infection and is mediated by the dual oxidase BLI-3, which produces H2O2. RNAi (RNA interference) to reduce the amount of BLI-3 results in a significant increase in susceptibility to pathogens, suggesting BLI-3 has a role in the immune response. However, H2O2 by itself is not a potent antimicrobial ...


The Show Cave Of Diros Vs. Wild Caves Of Peloponnese, Greece - Distribution Patterns Of Cyanobacteria, Vasiliki Lamprinou, Daniel B. Danielidis, Adriani Pantazidou, Alexandra Oikonomou, Athena Economou-Amilli 2014 University of South Florida

The Show Cave Of Diros Vs. Wild Caves Of Peloponnese, Greece - Distribution Patterns Of Cyanobacteria, Vasiliki Lamprinou, Daniel B. Danielidis, Adriani Pantazidou, Alexandra Oikonomou, Athena Economou-Amilli

International Journal of Speleology

The karst cave ‘Vlychada’of Diros, one of the oldest show caves in Peloponnese, sustains extended phototrophic biofilms on various substrata – on rocks inside the cave including speleothems, and especially near the artificial lighting installation (‘Lampenflora’). After a survey of the main abiotic parameters (Photosynthetically Active Radiation -PAR, Temperature -T, Relative Humidity -RH, Carbon Dioxide -CO2) three clusters of sampling sites were revealed according to Principal Component Analysis (PCA): i) the water gallery section predominately influenced by CO2, ii) the dry passages influenced by RH and PAR, and iii) the area by the cave exit at the dry ...


Ammonia-Oxidizing Bacteria And Archaea Under Continuous Maize: The Influence Of Tillage, N Input And Aggregation On Abundance And Community Composition, Lauren Segal 2014 University of Nebraska - Lincoln

Ammonia-Oxidizing Bacteria And Archaea Under Continuous Maize: The Influence Of Tillage, N Input And Aggregation On Abundance And Community Composition, Lauren Segal

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Nitrification involves the oxidation of ammonium (NH4+) and is an important component of the overall N cycle. Nitrification occurs in two steps; first by oxidizing ammonium to nitrite, and then to nitrate. The first step is often the rate limiting step. Until recently ammonia-oxidizing bacteria (AOB) were thought to be the sole contributors to this process; however, the discovery of crenarchaeota, ammonia-oxidizing archaea (AOA), in marine environments has led to further study of their role in nitrification. Current literature supports the dominance of AOA over AOB in terrestrial ecosystems; however, little is known about what drives their abundance. To ...


Probiotic Therapy For Heart Failure: Investigating The Potential Anti-Hypertrophic Properties Of Probiotics, Grace L. Ettinger 2014 Western University

Probiotic Therapy For Heart Failure: Investigating The Potential Anti-Hypertrophic Properties Of Probiotics, Grace L. Ettinger

University of Western Ontario - Electronic Thesis and Dissertation Repository

Heart failure patients face a five-year 50% mortality rate, due to impaired cardiac function and hypertrophy of the heart. Probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host. Considering the established cardiovascular benefits of some probiotics, including reduction of cholesterol and hypertension, it was hypothesized that probiotics can improve outcomes of heart failure. Probiotics or controls were administered orally to an animal model for heart failure. Those receiving probiotics showed a significant improvement in cardiac hypertrophy and an attenuation of heart failure compared to control. No changes in gut microbial composition occurred ...


Understanding The Factors Affecting Microbiological Quality Of Wheat Milled Products: From Wheat Fields To Milling Operations, Luis E. Sabillón Galeas 2014 University of Nebraska - Lincoln

Understanding The Factors Affecting Microbiological Quality Of Wheat Milled Products: From Wheat Fields To Milling Operations, Luis E. Sabillón Galeas

Dissertations & Theses in Food Science and Technology

Although regarded as a low-risk commodity, wheat flour-based mixes have been implicated in several food safety incidents. The present thesis is a compilation of five scientific manuscripts on the effect of weather variation, milling steps and implementation of pre-milling interventions on the microbiological quality and safety of wheat and milled products. The first manuscript is a review of the microbiological quality and safety of wheat-based products. Despite the low water activity, wheat flour may harbor dormant but viable microorganisms, which could lead to safety concerns when flour is used to produce refrigerated dough products. The second manuscript illustrates the effect ...


Interactions Of Various Bacterial Populations With Chemical And Physical Factors From Seasonal Inputs And Outputs Of Retention Ponds, Felicia A. Krelwitz 2014 Governors State University

Interactions Of Various Bacterial Populations With Chemical And Physical Factors From Seasonal Inputs And Outputs Of Retention Ponds, Felicia A. Krelwitz

Student Theses

Retention ponds at Governors State University play an important role in collecting and treating storm water runoff before leaving campus and entering Thorn Creek. Many chemical and physical factors influence the diversity of bacterial populations in freshwater ecosystems. The main objective of this study was to compare the interactions of various bacterial populations with chemical and physical factors from seasonal inputs and outputs of Governors State University retention ponds before being discharged into Thorn Creek. The retention ponds studied include the Café Settling pond, Café pond and Beaver pond. Water and sediment were collected from inputs and outputs every other ...


Fty720 (Fingolimod) Provides Insight Into The Molecular Mechanisms Of Multiple Sclerosis, Madelyn Elizabeth Crawford 2014 University of Tennessee, Knoxville

Fty720 (Fingolimod) Provides Insight Into The Molecular Mechanisms Of Multiple Sclerosis, Madelyn Elizabeth Crawford

Pursuit - The Journal of Undergraduate Research at the University of Tennessee

Multiple sclerosis (MS) is a neurodegenerative disorder caused by a prolonged immune- mediated inflammatory response that targets myelin. Nearly all of the drugs approved for the treatment of MS are general immunosuppressants or only function in symptom management. The oral medication fingolimod, however, is reported to have direct therapeutic effects on cells of the central nervous system in addition to immunomodulatory functions. Fingolimod is known to interact with sphingosine-1-phosphate (S1P) receptors, and the most widely- accepted theory for its mechanism of action is functional antagonism of the receptor. This review examines significant neuromodulatory effects achieved by functional antagonism of the ...


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