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Dopamine Levels In The Brain Of Rat Models Of Human Rheumatoid Arthritis, Amelia Stinson 2017 University of the Incarnate Word

Dopamine Levels In The Brain Of Rat Models Of Human Rheumatoid Arthritis, Amelia Stinson

Theses & Dissertations

Research Focus. Rheumatoid arthritis (RA) is a chronic, debilitating, autoimmune disease that causes the destruction of bone tissue and the articular structures of joints. At least 30% of RA patient populations have cognitive impairment. Acidic dopamine (DA) is the principal neuroimmunotransmitter that links the central nervous system and peripheral nervous system together. The aim of the present study was to determine the levels of DA and its two acidic metabolites: 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in arthritic induced rats, and whether their levels vary across four different parts of the brain: amygdala (AMG), front cerebral cortex (CX ...


Characterization Of Notch1 And Pi3k-Pten-Akt/Mtor Pathway Interaction In Head And Neck Squamous Cell Carcinoma, Kyriante' Henry 2017 The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences

Characterization Of Notch1 And Pi3k-Pten-Akt/Mtor Pathway Interaction In Head And Neck Squamous Cell Carcinoma, Kyriante' Henry

UT GSBS Dissertations and Theses (Open Access)

Head and neck squamous cell carcinoma (HNSCC) affects various mucosal sites of the upper aerodigestive tract, including the nasal and oral cavities, the nasopharynx, and the oropharynx. More than five hundred thousand new cases of HNSCC occurred in 2011 alone, with 50,000 reported cases in the United States. This trend made HNSCC the seventh most common non-skin cancer worldwide (Ferlay et al., 2015). Although significant epidemiological and pathological advancements have been made, survival rates have not improved much over the last 40 years, leaving a mortality rate that remains at approximately 50%. An unbiased drug screen demonstrated that HNSCC ...


Surveillance Of Wisconsin Organisms For Trends In Antimicrobial Resistance And Epidemiology (Swotare): Epidemiologic Correlates For 2016 Surveillance Isolates, Erik Munson, Heather Zeman, Erin Hueppchen 2017 Marquette University

Surveillance Of Wisconsin Organisms For Trends In Antimicrobial Resistance And Epidemiology (Swotare): Epidemiologic Correlates For 2016 Surveillance Isolates, Erik Munson, Heather Zeman, Erin Hueppchen

Clinical Lab Sciences Faculty Research and Publications

Background: The Centers for Disease Control and Prevention advocate data collection and monitoring as one facet of a comprehensive approach to combat antimicrobial resistance in the United States. However, a paucity of such data exists at the local/state level for common disease-causing organisms.

Methods: To begin to characterize epidemiologic correlates of antibacterial resistance in Wisconsin, data analyses were performed with respect to isolates in the Surveillance of Wisconsin Organisms for Trends in Antimicrobial Resistance and Epidemiology (SWOTARE) 2016 collection. In addition to submitting isolates of Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, and Streptococcus pneumoniae, participating laboratories were also requested ...


Testing Vert™ Accelerometer To Identify Validity And Reliability When Compared To Switch Mat, Tara McDonald 2017 East Tennessee State University

Testing Vert™ Accelerometer To Identify Validity And Reliability When Compared To Switch Mat, Tara Mcdonald

Undergraduate Honors Theses

This present study was intended to identify the reliability and validity of the Vert™ device when compared to a Switch mat. Vert is a wireless device intended to measure jump count and jump height through an application on a smartphone or tablet and the Switch mat provides jump height using wireless sensors. Jump height is an important factor in many sports such as volleyball and basketball and it is important to have devices that coaches and trainers can use for testing that they can rely on. If this device is found to be valid and reliable, coaches and trainers could ...


Exogenous Ubiquitin: Role In Myocardial Inflammation And Remodeling Post- Ischemia/Reperfusion Injury, Stephanie Scofield 2017 East Tennessee State University

Exogenous Ubiquitin: Role In Myocardial Inflammation And Remodeling Post- Ischemia/Reperfusion Injury, Stephanie Scofield

Electronic Theses and Dissertations

Sympathetic stimulation occurs in the heart after injuries such as ischemia/reperfusion (I/R) and myocardial infarction and affects myocardial remodeling. Prolonged sympathetic stimulation can result in myocardial dysfunction through its effects on cardiac myocyte apoptosis and myocardial fibrosis. Ubiquitin (UB) is well known for its role of tagging old or damaged proteins for degradation via the UB-proteosome pathway. The role of exogenous UB however, is not fully understood. Previously, our lab showed that β-adrenergic receptor (β-AR) stimulation increased levels of extracellular UB in the conditioned media of adult rat ventricular myocytes and that UB inhibits β-AR-stimulated apoptosis. This study ...


Intracellular Signaling And Trafficking In Cancer: Role Of Rab5-Gtpase In Migration And Invasion Of Breast Cells, Nicole Porther 2017 Biological Sciences

Intracellular Signaling And Trafficking In Cancer: Role Of Rab5-Gtpase In Migration And Invasion Of Breast Cells, Nicole Porther

Nicole Porther

Metastasis is characterized pathologically by uncontrolled cell invasion, proliferation, migration and angiogenesis. Steroid hormones, such as estrogen, and growth factors, which include insulin growth factor I/II (IGF-1/IGF-2) therapy has been associated with most if not all of the features of metastasis. It has been determined that IGF-1 increases cell survival of cancer cells and potentiate the effect of E2 and other ligand growth factors on breast cancer cells. However not much information is available that comprehensively expounds on the roles of insulin growth factor receptor (IGFR) and Rab GTPases may play in breast cancer. The latter, Rab GTPases ...


Neuronal Degeneration And Short-Term Memory Impairment After Tbi, Obiamaraije Igwe 2017 University of Louisville

Neuronal Degeneration And Short-Term Memory Impairment After Tbi, Obiamaraije Igwe

Posters-at-the-Capitol

Traumatic brain injury (TBI) was associated with impaired short-term memory with causes of vehicle accidents and falls. Protein plaques containing fibrinogen (Fg), are associated with memory loss. After TBI, Fg in blood was higher than normal (>~2 mg/ml), which resulted in increased Fg in extravascular space. Therefore, Fg bonded to its endothelial receptor intercellular adhesion molecule-1 (ICAM-1). Fg then interacted with cellular prion protein (PrPC), which had a strong effect on the loss of memory and cognition. Mechanisms of Fg and PrPC complex formation and its functional implication are not known. This present study tested the level of Fg-PrPC ...


Answer To November Photo Quiz: Campylobacter Jejuni Pleuritis, Robin Olson, Kimber L. Munson, Maureen Napierala, Erik Munson 2017 Wheaton Franciscan Laboratory

Answer To November Photo Quiz: Campylobacter Jejuni Pleuritis, Robin Olson, Kimber L. Munson, Maureen Napierala, Erik Munson

Clinical Lab Sciences Faculty Research and Publications

No abstract provided.


Photo Quiz: A 69-Year-Old Immunosuppressed Male With Chest Pain And Shortness Of Breath, Robin Olson, Kimber L. Munson, Maureen Napierala, Erik Munson 2017 Wheaton Franciscan Laboratory

Photo Quiz: A 69-Year-Old Immunosuppressed Male With Chest Pain And Shortness Of Breath, Robin Olson, Kimber L. Munson, Maureen Napierala, Erik Munson

Clinical Lab Sciences Faculty Research and Publications

No abstract provided.


A Brief Guide To Striped Bass Ecology & Management In Chesapeake Bay, Mary C. Fabrizio, Troy D. Tuckey, Susanna Musick 2017 Virginia Institute of Marine Science

A Brief Guide To Striped Bass Ecology & Management In Chesapeake Bay, Mary C. Fabrizio, Troy D. Tuckey, Susanna Musick

Reports

Chesapeake Bay striped bass support important recreational fisheries along the US Atlantic coast; in the late 1970s, the population of striped bass collapsed as a result of overfishing and poor water quality in rivers used for spawning and rear-ing of young. Informed by stock assessments, strict management regulations were enacted in the mid-1980s and early 1990s; these highly effective regulations resulted in the recovery of the population in 1995. A key to the successful recov-ery of the Atlantic coast striped bass was the wide range of ages of spawning females and the associated differences in spawning behavior among ages. Age ...


Molecular Diagnostics Update For The Emerging (If Not Already Widespread) Sexually Transmitted Infection Agent Mycoplasma Genitalium: Just About Ready For Prime Time, Munson Erik 2017 Marquette University

Molecular Diagnostics Update For The Emerging (If Not Already Widespread) Sexually Transmitted Infection Agent Mycoplasma Genitalium: Just About Ready For Prime Time, Munson Erik

Clinical Lab Sciences Faculty Research and Publications

Mycoplasma genitalium is an important and emerging agent of sexually transmitted infection in females and males, carrying the potential for postinfection genital tract sequelae. Past efforts to identify this organism on a routine basis, which were problematic due to the fastidious nature of the bacterium and its antigenic intricacies, have recently become supplemented by molecular diagnostics. A number of these assays are available commercially. This minireview describes the format and performance indices of a number of M. genitalium DNA- and RNA-based amplification assays; many of these assays have contributed to an improved clinical and epidemiologic understanding of this organism.


Experimental In-Vivo Models Used In Fat Grafting Research For Volume Augmentation In Soft Tissue Reconstruction, Jorge Lujan-Hernandez, Raghu Appasani, Kylee Sullivan, Leah Siegel-Reamer, Janice F. Lalikos 2017 University of Massachusetts Medical School

Experimental In-Vivo Models Used In Fat Grafting Research For Volume Augmentation In Soft Tissue Reconstruction, Jorge Lujan-Hernandez, Raghu Appasani, Kylee Sullivan, Leah Siegel-Reamer, Janice F. Lalikos

Open Access Articles

As the popularity of fat grafting research increases, animal models are being used as the source of pre-clinical experimental information for discovery and to enhance techniques. To date, animal models used in this research have not been compared to provide a standardized model. We analyzed publications from 1968-2015 to compare published accounts of animal models in fat grafting research. Data collected included: species used, graft characteristics (donor tissue, recipient area, amount injected, injection technique), time of sacrifice and quantification methods. Mice were most commonly used (56% of studies), with the "athymic nude" strain utilized most frequently (44%). Autologous fat was ...


Identification Of Stiffness-Induced Signalling Mechanisms In Cells From Patent And Fused Sutures Associated With Craniosynostosis., Sara Barreto, Arlyng Gonzalez Vazquez, Andrew R. Cameron, Fergal J. O'Brien, Dylan J Murray 2017 Royal College of Surgeons in Ireland

Identification Of Stiffness-Induced Signalling Mechanisms In Cells From Patent And Fused Sutures Associated With Craniosynostosis., Sara Barreto, Arlyng Gonzalez Vazquez, Andrew R. Cameron, Fergal J. O'Brien, Dylan J Murray

Anatomy Articles

Craniosynostosis is a bone developmental disease where premature ossification of the cranial sutures occurs leading to fused sutures. While biomechanical forces have been implicated in craniosynostosis, evidence of the effect of microenvironmental stiffness changes in the osteogenic commitment of cells from the sutures is lacking. Our aim was to identify the differential genetic expression and osteogenic capability between cells from patent and fused sutures of children with craniosynostosis and whether these differences are driven by changes in the stiffness of the microenvironment. Cells from both sutures demonstrated enhanced mineralisation with increasing substrate stiffness showing that stiffness is a stimulus capable ...


Evaluation Of Xpert Mtb/Rif Testing For Rapid Diagnosis Of Childhood Pulmonary Tuberculosis In Children By Xpert Mtb/Rif Testing Of Stool Samples In A Low Resource Setting, Zahra Hasan, Sadia Shakoor, Fehmina Arif, Aisha Mehnaz, Alnoor Akber, Marium Haider, Akber Kanji, Rumina Hasan 2017 Aga Khan University

Evaluation Of Xpert Mtb/Rif Testing For Rapid Diagnosis Of Childhood Pulmonary Tuberculosis In Children By Xpert Mtb/Rif Testing Of Stool Samples In A Low Resource Setting, Zahra Hasan, Sadia Shakoor, Fehmina Arif, Aisha Mehnaz, Alnoor Akber, Marium Haider, Akber Kanji, Rumina Hasan

Department of Pathology and Laboratory Medicine

Objective: Children with tuberculosis (TB) remain underdiagnosed due to difculty in testing for Mycobacterium tuberculosis (MTB) infection. We evaluated the Xpert MTB/RIF assay for respiratory and stool testing in children for pulmonary TB through a cross-sectional study at tertiary care facilities in Karachi, Pakistan. Fifty children aged 0–15 years screened by a modifed Kenneth-Jones (KJ) score were included. Mycobacterial culture of respiratory samples was the microbiological standard against stool Xpert TB results. All positive TB cases were compared against a treatment response standard (TRS).
Results: Twelve study subjects were diagnosed by Xpert TB and nine by MTB culture ...


Point Of Care Diagnosis Of Multiple Schistosome Parasites: Species-Specific Dna Detection In Urine By Loop-Mediated Isothermal Amplification (Lamp), Nilanjan Lodh, Kei Mikita, Kwabena M. Bosompem, William K. Anyan, Joseph K. Quartey, Joseph Otchere, Clive J. Shiff 2017 Marquette University

Point Of Care Diagnosis Of Multiple Schistosome Parasites: Species-Specific Dna Detection In Urine By Loop-Mediated Isothermal Amplification (Lamp), Nilanjan Lodh, Kei Mikita, Kwabena M. Bosompem, William K. Anyan, Joseph K. Quartey, Joseph Otchere, Clive J. Shiff

Clinical Lab Sciences Faculty Research and Publications

Schistosomes are easily transmitted and high chance of repeat infection, so if control strategies based on targeted mass drug administration (MDA) are to succeed it is essential to have a test that is sensitive, accurate and simple to use. It is known and regularly demonstrated that praziquantel does not always eliminate an infection so in spite of the successes of control programs a residual of the reservoir survives to re-infect snails. The issue of diagnostic sensitivity becomes more critical in the assessment of program effectiveness. While serology, such as antigen capture tests might improve sensitivity, it has been shown that ...


Results Of A Local Combination Therapy Antibiogram For Pseudomonas Aeruginosa Isolates: Is Double Worth The Trouble?, Matthew Song, Thomas J. Dilworth, Erik Munson, Jim Davis, Ramy Elshaboury 2017 Norton Healthcare

Results Of A Local Combination Therapy Antibiogram For Pseudomonas Aeruginosa Isolates: Is Double Worth The Trouble?, Matthew Song, Thomas J. Dilworth, Erik Munson, Jim Davis, Ramy Elshaboury

Clinical Lab Sciences Faculty Research and Publications

Purpose:

To determine the frequency at which fluoroquinolones and aminoglycosides demonstrate in vitro activity against non-urinary, non-skin/skin structure Pseudomonas aeruginosa isolates exhibiting decreased susceptibilities to one or more β-lactam agents.

Methods:

β-lactam-non-susceptible P. aeruginosa isolates recovered from blood, bone, lower respiratory tract, pleural fluid, cerebrospinal fluid, or peritoneal fluid cultures between October 2010 and October 2014 were reviewed from four community hospitals within a single health-system. Only the first isolate per patient was included for analysis. The likelihood that each isolate was susceptible to a non-β-lactam antimicrobial was then determined and summarized within a combination antibiogram.

Results:

In total ...


Intracellular Signalling Crosstalk In The Differentiation Of F9 Cells Into Extraembryonic Endoderm, Tina Nicole Cuthbert 2017 The University of Western Ontario

Intracellular Signalling Crosstalk In The Differentiation Of F9 Cells Into Extraembryonic Endoderm, Tina Nicole Cuthbert

Electronic Thesis and Dissertation Repository

Mouse F9 cells differentiate into primitive extraembryonic endoderm (PrE) with retinoic acid (RA) treatment, resulting in up-regulation of Gata6, which when translated directly activates Wnt6. Canonical Wnt signalling is required for PrE differentiation, and this, like most developmental processes, requires input from one or more additional pathways, including hedgehog (Hh). The Hh pathway is regulated by GATA6, and crosstalks positively/negatively with Wnt signalling. Ihh up-regulation in F9 cells accompanies PrE induction, but a role for GATA6 or Hh pathway activation in obligatory Wnt/ß-catenin signalling is not known. To address this, I show that RA induces Ihh and altered ...


The Role Of The Epithelial-To-Mesenchymal Transition (Emt) In Lung Cancer Progression, David H. Peng 2017 The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences

The Role Of The Epithelial-To-Mesenchymal Transition (Emt) In Lung Cancer Progression, David H. Peng

UT GSBS Dissertations and Theses (Open Access)

Lung cancer is the leading cause of cancer-related deaths due to conventional therapy resistance and metastatic disease, therefore understanding the mechanisms governing these biological functions is vital for improving patient survival. Approximately 30% of patients with the adenocarcinoma histologic subset of lung cancer possess an activating KRAS mutation, characterized by a lack of response to chemotherapies with a poor overall 5-year survival rate. Despite the mutational frequency, KRAS remains a challenge to pharmacologically inhibit and current drugs undergoing clinical trials that target specific downstream effector proteins of KRAS, such as MEK inhibitors, have failed to produce significant clinical benefits. Previous ...


Preclinical Development Of Therapeutic Strategies Against Triple-Negative And Inflammatory Breast Cancer, Angie M. Torres-Adorno 2017 The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences

Preclinical Development Of Therapeutic Strategies Against Triple-Negative And Inflammatory Breast Cancer, Angie M. Torres-Adorno

UT GSBS Dissertations and Theses (Open Access)

Triple-negative (TNBC) and inflammatory (IBC) breast cancer are the most aggressive forms of breast cancer, accounting for 20% and 10% of cancer-related deaths, respectively. Among IBC cases, 30% are additionally classified with TNBC molecular pathology, a diagnosis that significantly worsens patient’s prognosis. The current lack of TNBC and IBC molecular understanding prevents the development of effective therapeutic strategies. To identify effective treatments, we explored aberrant apoptosis pathways and cell membrane fluidity as novel therapeutic targets.

We first identified an effective therapeutic strategy against TNBC and IBC by pro-apoptotic protein NOXA-mediated inhibition of the anti-apoptotic protein MCL1 following inhibition of ...


The Effect Of Hemodynamic Force On The Maturation Of Blood Vessels During Embryogenesis, Rachel Lee Padget 2017 Missouri State University

The Effect Of Hemodynamic Force On The Maturation Of Blood Vessels During Embryogenesis, Rachel Lee Padget

MSU Graduate Theses

Throughout embryonic development, blood vessels are derived from endothelial cells by way of vasculogenesis. During angiogenesis, vessels remodel to form a hierarchy of large-diameter arteries that branch into small-diameter capillaries. In this maturation, vessels respond to unidentified signaling events to become surrounded with an outer layer of vascular smooth muscle cells (vSMCs). This results in arteries that have a thick vSMC layer, veins that have a thin vSMC layer, and capillaries that have a very thin or absent vSMC layer. What remains to be determined is the cause of the thicker layer of vSMCs around proximal arteries. Previous studies have ...


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