Open Access. Powered by Scholars. Published by Universities.®

Laboratory and Basic Science Research Commons

Open Access. Powered by Scholars. Published by Universities.®

900 Full-Text Articles 1,928 Authors 227,800 Downloads 141 Institutions

All Articles in Laboratory and Basic Science Research

Faceted Search

900 full-text articles. Page 6 of 35.

22 - Bugs In Bugs, Josemaria Garcia 2018 University of North Georgia

22 - Bugs In Bugs, Josemaria Garcia

Georgia Undergraduate Research Conference (GURC)

The primary purpose of the Bugs in Bugs project is to measure a student’s ability to go complete a research report and to observe demonstrations of key fundamental research aspects such as understanding scientific works, collecting and entering data, analyzing and creating graphs, and overall how to write a lab report. Working with Principles of Biology II and Microbiology classes, students were provided with a topic and methodology respective to their class; however, groups within the classes had the freedom to create their own hypotheses and theses, choose how to collect and interpret data, make charts and graphs, and ...


Mycobacterium Tuberculosis Inhibitors: Action And Resistance, Pamela K. Garcia-Moreno 2018 Florida International University

Mycobacterium Tuberculosis Inhibitors: Action And Resistance, Pamela K. Garcia-Moreno

FIU Electronic Theses and Dissertations

Tuberculosis, an infectious disease caused by Mycobacterium tuberculosis, has been a global health problem for years. The emergence of drug resistance in this organism generates the necessity of exploring novel targets and developing new drugs. Topoisomerases are enzymes found in all kingdoms of life responsible for overcoming the topological barriers encountered during essential cellular processes. The genomes of mycobacteria encode only one type IA topoisomerase (MtopI), which has been validated as a novel TB drug target. The goal of this study is to obtain new information on the mechanism and resistance of endogenous and synthetic inhibitors of MtopI.

Rv1495 is ...


The Interplay Between Polarity Regulators, Calcium, And The Actin Cytoskeleton During Tip Growth, Carlisle Bascom Jr 2018 University of Massachusetts Amherst

The Interplay Between Polarity Regulators, Calcium, And The Actin Cytoskeleton During Tip Growth, Carlisle Bascom Jr

Doctoral Dissertations

Plant cell growth is a meticulously regulated process whereby the cell wall is selectively loosened to allow for turgor-pressure driven expansion. The rate of expansion must equal delivery of new material, or the cell will lyse. In many plant cells, this process happens diffusely around the cell. However, a number of plant cells have anisotropic shapes that require exquisite spatial control of secretion. One simple example of anisotropic patterning is tip growth; highly polarized cell expansion utilized by pollen tubes, root hairs, and moss protonemata. Investigating the role various molecules have in tip growth sheds light on how plant cells ...


Adapting Cell-Free Protein Synthesis As A Platform Technology For Education, Grace W. Chu, Max Z. Levine, Nicole E. Gregorio, Javin P. Oza 2018 California State University, Los Angeles

Adapting Cell-Free Protein Synthesis As A Platform Technology For Education, Grace W. Chu, Max Z. Levine, Nicole E. Gregorio, Javin P. Oza

STAR (STEM Teacher and Researcher) Presentations

Cell-free protein synthesis (CFPS) has emerged as an enabling biotechnology for research and biomanufacturing as it allows for the production of protein without the need for a living cell. Applications of CFPS include the construction of libraries for functional genomics and structural biology, the production of personalized medicine, and the expression of virus-like particles. The absence of a cell wall provides an open platform for direct manipulation of the reaction conditions and biological machinery. This project focuses on adapting the CFPS biotechnology to the classroom, making a hands-on bioengineering approach to learning protein synthesis accessible to students grades K-16 through ...


Personal Safety Culture: A New Measure For General Aviation Pilots, Bradley S. Baugh, Kimberly M. Bracewell, Urara Takano, Mattie N. Milner, Emily C. Anania, Nadine K. Ragbir, Mudhur Bharat Gupta, Diego M. Garcia, Drishti O. Valecha, Daniel A. Marte, Scott R. Winter, Stephen Rice 2018 Embry-Riddle Aeronautical University

Personal Safety Culture: A New Measure For General Aviation Pilots, Bradley S. Baugh, Kimberly M. Bracewell, Urara Takano, Mattie N. Milner, Emily C. Anania, Nadine K. Ragbir, Mudhur Bharat Gupta, Diego M. Garcia, Drishti O. Valecha, Daniel A. Marte, Scott R. Winter, Stephen Rice

National Training Aircraft Symposium (NTAS)

Safety culture has been a subject of research for over three decades and is now widely accepted as a critical component of organizational safety programs both domestically and internationally. Through the development of a healthy safety culture, aviation organizations can improve safety processes, reduce mishaps, and mitigate risk more effectively. This is done through the holistic team efforts of an organization’s members and the organization’s leadership. How about aviators who are not part of an organization? Is it possible to identify a personal safety culture defined outside of the traditional organization? And, is it possible to create an ...


Promoting Adherence To Best Practice Related To Urine Reflex To Culture Testing, Maykel Verdecia Alonso BA,RN-CPHN 2018 The University of San Francisco

Promoting Adherence To Best Practice Related To Urine Reflex To Culture Testing, Maykel Verdecia Alonso Ba,Rn-Cphn

Nursing and Health Professions Faculty Research and Publications

Over a two week period, the infection control nurse, from a trauma designated facility located in norther California, planned a practice improvement project in collaboration with the laboratory microsystem to educate referring physicians and increase adherence to the Centers for Disease Control (CDC) protocol, which delineates recommended best practices related to urine reflex to culture testing. Urinalysis (UA) is a test that triggers a reflex urine culture when pyuria is present. Frequent urine culturing without the presence of pyuria can cause unnecessary treatment with antimicrobials. From January 2017 to December 2017, 10% of UAs from 200 urine samples from asymptomatic ...


Computational Analysis Of Genomic Variants Affecting Predicted Microrna:Target Interactions In Prostate Cancer., Angélica Paola Hernández Pérez 2018 Keck Graduate Institute, KGI

Computational Analysis Of Genomic Variants Affecting Predicted Microrna:Target Interactions In Prostate Cancer., Angélica Paola Hernández Pérez

KGI Theses and Dissertations

Prostate cancer (PCa) is the most common cancer of men in the United States and is third only to lung and colon as a cause of cancer death. Clinical behavior of the disease is variable and the combination of prostate-specific antigen (PSA) screening and Gleason score staging are currently the best available molecular and pathology tools to predict outcomes. Cancer biology research establishes microRNAs (miRNAs) as key molecular components in both normal and pathological states. Thus, elucidating miRNAs perturbed by genomic alterations will expand our understanding of the molecular taxonomy of PCa with the aim to complement current practices in ...


Investigation Of Zebrafish Larvae Behavior As Precursor For Suborbital Flights: Feasibility Study, Pedro Llanos, Kristina Andrijauskaite, Mark Rubinstein, Sherine S.L. Chan 2018 Embry-Riddle Aeronautical University - Daytona Beach

Investigation Of Zebrafish Larvae Behavior As Precursor For Suborbital Flights: Feasibility Study, Pedro Llanos, Kristina Andrijauskaite, Mark Rubinstein, Sherine S.L. Chan

Pedro J. Llanos (www.AstronauticsLlanos.com)


Suborbital spaceflights, carrying scientific payloads, allow scientists not only to test the feasibility of their payloads, but they also provide the basis for refining scientific hypotheses to be later tested on the International Space Station (ISS). Therefore, it is essential to establish robust pre-flight procedures in order to take advantage of this unique research platform to facilitate payload delivery. In the present study, we assessed zebrafish larvae behavior as a precursor for the future suborbital spaceflight involving research on the musculoskeletal system. Zebrafish larvae were exposed to the same physiological stressors they would encounter during suborbital spaceflight: alterations in light ...


Importance Of Welfare And Ethics Competence Regarding Animals Kept For Scientific Purposes To Veterinary Students In Australia And New Zealand, Teresa Collins, Amelia Cornish, Jennifer Hood, Chris Degeling, Andrew D. Fisher, Rafael Freire, Susan J. Hazel, Jane Johnson, Jennifer K.F. Lloyd, Clive J.C. Phillips, Vicky Tzioumis, Paul D. McGreevy 2018 Murdoch University

Importance Of Welfare And Ethics Competence Regarding Animals Kept For Scientific Purposes To Veterinary Students In Australia And New Zealand, Teresa Collins, Amelia Cornish, Jennifer Hood, Chris Degeling, Andrew D. Fisher, Rafael Freire, Susan J. Hazel, Jane Johnson, Jennifer K.F. Lloyd, Clive J.C. Phillips, Vicky Tzioumis, Paul D. Mcgreevy

Professional Science Research Ethics Collection

Veterinarians are in a strong position of social influence on animal-related issues. Hence, veterinary schools have an opportunity to raise animal health and welfare standards by improving veterinary students’ animal welfare and ethics (AWE) education, including that related to animals used for scientific purposes. A survey of 818 students in the early, mid, and senior stages of their courses at all eight veterinary schools across Australia and New Zealand was undertaken on their first day of practice (or Day One Competences) to explore how veterinary students viewed the importance of their competence in the management of welfare and ethical decision-making ...


Bioethics (Gcsu), Kasey Karen, Jennifer Townes 2018 Georgia College and State University

Bioethics (Gcsu), Kasey Karen, Jennifer Townes

Biological Sciences Grants Collections

This Grants Collection for Bioethics was created under a Round Ten ALG Textbook Transformation Grant.

Affordable Learning Georgia Grants Collections are intended to provide faculty with the frameworks to quickly implement or revise the same materials as a Textbook Transformation Grants team, along with the aims and lessons learned from project teams during the implementation process.

Documents are in .pdf format, with a separate .docx (Word) version available for download. Each collection contains the following materials:

  • Linked Syllabus
  • Initial Proposal
  • Final Report


Spatial And Temporal Patterns Of Arctic Nearshore Fish Community And Food Web Structures, Mark B. Barton 2018 Florida International University

Spatial And Temporal Patterns Of Arctic Nearshore Fish Community And Food Web Structures, Mark B. Barton

FIU Electronic Theses and Dissertations

Climate change and increasing anthropogenic activities are causing rapid changes to environmental and ecological processes in the Arctic Ocean. To better understand these changes, scientists have increased research efforts in these regions, but to date the number of studies on Arctic nearshore habitats are lacking. My dissertation responds to the paucity of information and investigates patterns in Arctic nearshore fish communities and food webs to gain insight to how these ecosystems may shift as these changes continue. I used multivariate statistical analysis to examine patterns in community structure and composition to determine that Arctic nearshore fish communities are largely driven ...


Mapping Molecular Datasets Back To The Brain Regions They Are Extracted From: Remembering The Native Countries Of Hypothalamic Expatriates And Refugees, Arshad M. Khan, Alice H. Grant, Anais Martinez, Gully APC Burns, Brendan S. Thatcher, Vishwanath T. Anekonda, Benjamin W. Thompson, Zachary S. Roberts, Daniel H. Moralejo, James E. Blevins 2018 University of Texas at El Paso

Mapping Molecular Datasets Back To The Brain Regions They Are Extracted From: Remembering The Native Countries Of Hypothalamic Expatriates And Refugees, Arshad M. Khan, Alice H. Grant, Anais Martinez, Gully Apc Burns, Brendan S. Thatcher, Vishwanath T. Anekonda, Benjamin W. Thompson, Zachary S. Roberts, Daniel H. Moralejo, James E. Blevins

Arshad M. Khan, Ph.D.

This article, which includes novel unpublished data along with commentary and analysis,
focuses on approaches to link transcriptomic, proteomic, and peptidomic datasets mined from
brain tissue to the original locations within the brain that they are derived from using digital atlas
mapping techniques. We use, as an example, the transcriptomic, proteomic and peptidomic
analyses conducted in the mammalian hypothalamus. Following a brief historical overview, we
highlight studies that have mined biochemical and molecular information from the hypothalamus
and then lay out a strategy for how these data can be linked spatially to the mapped locations in a
canonical brain atlas ...


The Zinc Transporter Zipt-7.1 Regulates Sperm Activation In Nematodes, Yanmei Zhao, Chieh-Hsiang Tan, Amber Krauchunas, Andrea Scharf, Nicholas Dietrich, Kurt Warnhoff, Zhiheng Yuan, Marina Druzhinina, Sam Guoping Gu, Long Miao, Andrew Singson, Ronald E Ellis, Kerry Kornfeld 2018 Rowan University

The Zinc Transporter Zipt-7.1 Regulates Sperm Activation In Nematodes, Yanmei Zhao, Chieh-Hsiang Tan, Amber Krauchunas, Andrea Scharf, Nicholas Dietrich, Kurt Warnhoff, Zhiheng Yuan, Marina Druzhinina, Sam Guoping Gu, Long Miao, Andrew Singson, Ronald E Ellis, Kerry Kornfeld

School of Osteopathic Medicine Faculty Scholarship

Sperm activation is a fascinating example of cell differentiation, in which immotile spermatids undergo a rapid and dramatic transition to become mature, motile sperm. Because the sperm nucleus is transcriptionally silent, this transition does not involve transcriptional changes. Although Caenorhabditis elegans is a leading model for studies of sperm activation, the mechanisms by which signaling pathways induce this transformation remain poorly characterized. Here we show that a conserved transmembrane zinc transporter, ZIPT-7.1, regulates the induction of sperm activation in Caenorhabditis nematodes. The zipt-7.1 mutant hermaphrodites cannot self-fertilize, and males reproduce poorly, because mutant spermatids are defective in responding ...


Biological Soil Crusts In A Northeastern Pine Barren: Composition And Ecological Effects, Jessica Gilbert 2018 Union College - Schenectady, NY

Biological Soil Crusts In A Northeastern Pine Barren: Composition And Ecological Effects, Jessica Gilbert

Honors Theses

Biological soil crusts (BSCs), otherwise known as cryptogamic soil crusts, biocrusts, or cyanobacterial crusts, are soil aggregations hosting diverse biotic communities. They are composed of cyanobacteria and algae, and generally have a covering of moss and/or lichen. BSCs are typically found in arid to semi-arid regions throughout the world, and are integral soil stabilizers, moisture retainers, and nitrogen fixers in these communities. Along with these factors, BSCs are able to impact germination and establishment of plants, either as an accompanying influence, or direct result of those listed above. BSCs have yet to be formally described in the inland northeastern ...


Light-Induced Expression Of A Blue Coral Protein In An Industrial Fungus, Lindsay Tomczak 2018 Union College

Light-Induced Expression Of A Blue Coral Protein In An Industrial Fungus, Lindsay Tomczak

Honors Theses

The ultimate goal of this research project is to improve the growth and structural characteristics of an Ecovative Design LLC (Green Island, NY) production strain to produce commercial biomaterials for packaging. These biomaterials are produced from renewable resources and can be easily broken down after they fulfill their purpose, unlike the commonly used materials today (such as Styrofoam). In an effort to quantify the light-reactivity of the fungus, a codon-optimized DNA sequence coding a blue chromoprotein was introduced and utilized as a visual reporter gene. Transcriptional controlling sequences were identified from orthologs to specific light-regulated genes and were combined with ...


Analysis Of Metallic Components Of Gsr From Various Types Of Ammunition And Firearms Utilizing An Sem-Edx, Jenna A. Campbell 2018 Duquesne University

Analysis Of Metallic Components Of Gsr From Various Types Of Ammunition And Firearms Utilizing An Sem-Edx, Jenna A. Campbell

Graduate Student Research Symposium

Gunshot residue (GSR) is a chemical reaction that occurs when a firearm is discharged, which emits a cloud of gases from the barrel of the gun. It consists of both the burned and unburned propellant and primer components. These components are either organic or inorganic in nature, respectively. The organic components are located in the gunpowder part of the bullet, while the inorganic components are located in the primer part of the bullet. The primer consists of lead, barium, and antimony. These elements are considered heavy metals. Additionally, lead, barium, and antimony are the three key elements found in the ...


The Wide Utility Of Rabbits As Models Of Human Diseases, Pedro J. Esteves, Yuxing Chen, Shuying Liu, Shan Lu, Michael Vaine, Shixia Wang, Rose Mage 2018 Universidade do Porto, Portugal

The Wide Utility Of Rabbits As Models Of Human Diseases, Pedro J. Esteves, Yuxing Chen, Shuying Liu, Shan Lu, Michael Vaine, Shixia Wang, Rose Mage

Open Access Articles

Studies using the European rabbit Oryctolagus cuniculus contributed to elucidating numerous fundamental aspects of antibody structure and diversification mechanisms and continue to be valuable for the development and testing of therapeutic humanized polyclonal and monoclonal antibodies. Additionally, during the last two decades, the use of the European rabbit as an animal model has been increasingly extended to many human diseases. This review documents the continuing wide utility of the rabbit as a reliable disease model for development of therapeutics and vaccines and studies of the cellular and molecular mechanisms underlying many human diseases. Examples include syphilis, tuberculosis, HIV-AIDS, acute hepatic ...


Effects Of Magnesium Deprivation On The Flora Of The Gastrointestinal Tract, Tricia Cooke 2018 The College at Brockport

Effects Of Magnesium Deprivation On The Flora Of The Gastrointestinal Tract, Tricia Cooke

Senior Honors Theses

Crohn’s disease, a pathological condition characterized by gastrointestinal (GI) inflammation and mucosal changes is often associated with hypomagnesemia resulting from changes in the GI mucosa and an increased GI transit rate. Furthermore, certain types of bacteria are also implicated in the formation of GI neoplasia and carcinogenesis. A variety of physiologic changes such as an increase in the systemic stress response, severity of the inflammatory response, or an exaggerated immune response is known to occur in Magnesium (Mg2+) deficient mice and rats. To understand to what extent Mg2+ deficiency could contribute to the aggravation of such diseases by ...


Schistosomiasis In The Wild: A Transcriptomics Perspective On Field-Derived Biomphalaria Pfeifferi And Schistosoma Mansoni, And Their Interactions, Sarah K. Buddenborg 2018 University of New Mexico - Main Campus

Schistosomiasis In The Wild: A Transcriptomics Perspective On Field-Derived Biomphalaria Pfeifferi And Schistosoma Mansoni, And Their Interactions, Sarah K. Buddenborg

Biology ETDs

Schistosomiasis, caused by trematodes in the genus Schistosoma, is a widespread neglected tropical disease with the species S. mansoni infecting over 100 million people. We aimed to better understand the snail host and parasite responses during intramolluscan stages of infection by performing dual RNA-Seq on field-collected snails with natural infections from western Kenya. We collected uninfected Biomphalaria pfeifferi, B. pfeifferi with a patent cercariae-producing S. mansoni infection, and B. pfeifferi exposed to field-collected S. mansoni at 1 and 3d (days post infection).

We first created a high-quality B. pfeifferi transcriptome to identify the snail response to S. mansoni infection. As ...


Modeling And Analyzing An Optogenetic System For Photoactivatable Protein Dissociation, Anvin Thomas, James Schaff 2018 University of Connecticut

Modeling And Analyzing An Optogenetic System For Photoactivatable Protein Dissociation, Anvin Thomas, James Schaff

Honors Scholar Theses

Computational modeling of cell-cell interactions can grant clues and can answer questions about an experiment, especially for observations about binding interactions and kinetics. This approach was used to investigate an interaction between a light-oxygen-voltage (LOV) domain and an engineered protein called Zdark (Zdk). The LOV domain is membrane-bound while Zdk is cytosolic. The LOV domain and Zdk bind strongly in dark (Kd 26.2 nM), and weakly upon exposure to blue light (Kd > 4 μM). Total internal reflection fluorescence (TIRF) images are acquired of Zdk, the fluorescent species bound to a mCherry tag, and the loss of fluorescence ...


Digital Commons powered by bepress