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Cyclin C Regulated Oxidative Stress Responsive Transcriptome In Mus Musculus Embryonic Fibroblasts, David C Stieg, Kai-Ti Chang, Katrina F Cooper, Randy Strich 2019 Rowan University

Cyclin C Regulated Oxidative Stress Responsive Transcriptome In Mus Musculus Embryonic Fibroblasts, David C Stieg, Kai-Ti Chang, Katrina F Cooper, Randy Strich

School of Osteopathic Medicine Faculty Scholarship

The transcriptional changes that occur in response to oxidative stress help direct the decision to maintain cell viability or enter a cell death pathway. Cyclin C-Cdk8 is a conserved kinase that associates with the RNA polymerase II Mediator complex that stimulates or represses transcription depending on the locus. In response to oxidative stress, cyclin C, but not Cdk8, displays partial translocation into the cytoplasm. These findings open the possibility that cyclin C relocalization is a regulatory mechanism governing oxidative stress-induced transcriptional changes. In the present study, the cyclin C-dependent transcriptome was determined and compared to transcriptional changes occurring in oxidatively ...


Crystal Structure Of Zymonic Acid And A Redetermination Of Its Precursor, Pyruvic Acid, Dominik Heger, Alexis J. Eugene, Sean R. Parkin, Marcelo I. Guzman 2019 University of Kentucky

Crystal Structure Of Zymonic Acid And A Redetermination Of Its Precursor, Pyruvic Acid, Dominik Heger, Alexis J. Eugene, Sean R. Parkin, Marcelo I. Guzman

Chemistry Faculty Publications

The structure of zymonic acid (systematic name: 4-hy­droxy-2-methyl-5-oxo-2,5-di­hydro­furan-2-carb­oxy­lic acid), C6H6O5, which had previously eluded crystallographic determination, is presented here for the first time. It forms by intra­molecular condensation of parapyruvic acid, which is the product of aldol condensation of pyruvic acid. A redetermination of the crystal structure of pyruvic acid (systematic name: 2-oxo­propanoic acid), C3H4O3, at low temperature (90 K) and with increased precision, is also presented [for the previous structure, see: Harata et al. (1977). Acta Cryst. B33, 210–212 ...


Anti-Human Cd9 Antibody Fab Fragment Impairs The Internalization Of Extracellular Vesicles And The Nuclear Transfer Of Their Cargo Proteins., Mark F. Santos, Germana Rappa, Jana Karbanová, Cheryl Vanier, Chikao Morimoto, Denis Corbeil, Aurelio Lorico 2019 Touro University Nevada

Anti-Human Cd9 Antibody Fab Fragment Impairs The Internalization Of Extracellular Vesicles And The Nuclear Transfer Of Their Cargo Proteins., Mark F. Santos, Germana Rappa, Jana Karbanová, Cheryl Vanier, Chikao Morimoto, Denis Corbeil, Aurelio Lorico

College of Osteopathic Medicine (TUN) Publications and Research

The intercellular communication mediated by extracellular vesicles (EVs) has gained international interest during the last decade. Interfering with the mechanisms regulating this cellular process might find application particularly in oncology where cancer cell-derived EVs play a role in tumour microenvironment transformation. Although several mechanisms were ascribed to explain the internalization of EVs, little is our knowledge about the fate of their cargos, which are crucial to mediate their function. We recently demonstrated a new intracellular pathway in which a fraction of endocytosed EV-associated proteins is transported into the nucleoplasm of the host cell via a subpopulation of late endosomes penetrating ...


Integrating Online Instruction And Hands-On Laboratory Activities For Summer Learning For Students Of Color: A Design Case In Forensic Science, Douglas Elrick, Jiaqi Yu, Connie Hargrave 2019 Iowa State University

Integrating Online Instruction And Hands-On Laboratory Activities For Summer Learning For Students Of Color: A Design Case In Forensic Science, Douglas Elrick, Jiaqi Yu, Connie Hargrave

Constance P. Hargrave

The popularity of TV shows such as Crime Scene Investigation (CSI) has generated high school students’ interest in forensics. Yet, forensic science is not commonly accessible to students, and especially students of color who often attend under-resourced high schools. This article presents the design, development, and evaluation of an online forensics course created for high school students of color who were a part of an informal science, technology, engineering, and mathematics (STEM) educational development program. Two essential elements guided the course design: the target learners (high school students of color) and integrating online instruction and hands-on laboratory activities involving real-world ...


Differential Roles Of Mammalian Target Of Rapamycin Complexes 1 And 2 In Migration Of Prostate Cancer Cells, Smrruthi Vaidegi Venugopal 2019 Clark Atlanta University

Differential Roles Of Mammalian Target Of Rapamycin Complexes 1 And 2 In Migration Of Prostate Cancer Cells, Smrruthi Vaidegi Venugopal

Electronic Theses & Dissertations Collection for Atlanta University & Clark Atlanta University

In this study, we investigated differential activation and the role of two mTOR complexes in cell migration of prostate cancer cells. Specific knock-down of endogenous RAPTOR and RICTOR by siRNA resulted in decreased cell migration in LNCaP, DU145, and PC3 cells indicating that both mTORC1 and mTORC2 are required for cell migration. EGF treatment induced the activation of both mTORC1 and mTORC2 as determined by complex-specific phosphorylation of mTOR protein. Specific knock-down or inhibition of Rac1 activity in PC3 cells blocked EGF-induced activation of mTORC2, but had no effect on mTORC1 activation. Furthermore, the over-expression of constitutively active Rac1 (Rac1Q61L ...


Establishment Of Crispr/Cas-9 Aided Knockout Of The Zic2 Gene In The African-American Prostate Cancer Cell Line E006aa-Pr, Janelle Moore 2019 Atlanta University Center

Establishment Of Crispr/Cas-9 Aided Knockout Of The Zic2 Gene In The African-American Prostate Cancer Cell Line E006aa-Pr, Janelle Moore

Electronic Theses & Dissertations Collection for Atlanta University & Clark Atlanta University

The largest U.S. cancer health disparity exists in prostate cancer, with African American men having the highest incidence and mortality rates. The present study evaluated the effects of ZIC2 and the underlying mechanisms in the E006 parental African-American cell line that produces tumors at accelerated growth rates because of the increase of ZIC2 genes in African-American males. We analyzed the experimental research that the overexpression of ZIC2 contributes to progression of prostate cancer. E006AA cells with overexpressed or suppressed ZIC2 were analyzed to determine phenotypic differences, PCR, cell proliferation and immunoblot assays. The expression levels of ZIC2 were analyzed ...


Htlv-1 Latency-Maintenance Factor P30ii Modulates Tax-Induced Nf-Kappa B Signaling Through A P53-Regulated Mechanism, Aditi Malu 2019 Southern Methodist University

Htlv-1 Latency-Maintenance Factor P30ii Modulates Tax-Induced Nf-Kappa B Signaling Through A P53-Regulated Mechanism, Aditi Malu

Biological Sciences Theses and Dissertations

The human T-cell leukemia virus type-1 (HTLV-1) infects CD4+ T-cells and is the etiological agent of adult T-cell leukemia/lymphoma (ATL), an aggressive and often-fatal lymphoproliferative neoplastic disease. The major transactivator protein, Tax, drives proviral gene expression and replication and plays a key role during the early-stage immortalization of T-cells by HTLV-1. Tax has also been shown to regulate many host cellular signaling pathways, including CREB/ATF, SRF, and NFκB. Another accessory viral protein p30II acts as a latency-maintenance factor and negatively regulates proviral gene expression and replication. The p30II protein counters Tax-dependent gene expression at the transcriptional ...


Jet Lag: What’S Light Got To Do With It?, Melissa Richardson 2019 Andrews University

Jet Lag: What’S Light Got To Do With It?, Melissa Richardson

Adventist Human-Subject Researchers Association

Jet lag results in memory loss and sleepiness. Current treatments for jet lag focus on symptoms and not the cause of jet lag; disrupted light environment. This study in mice, uncovered mechanisms of how light regulates jet lag recovery and memory. This work informs our future studies in humans.


A Discrepancy Between The Human Reference Genome (Grch37) And Transcriptome (Refseq) Results In The Incorrect Annotation Of A Clinically-Relevant Sequence Variant In Recql4, Lisa A. Lansdon 2019 Children's Mercy Kansas City

A Discrepancy Between The Human Reference Genome (Grch37) And Transcriptome (Refseq) Results In The Incorrect Annotation Of A Clinically-Relevant Sequence Variant In Recql4, Lisa A. Lansdon

Research Days

No abstract provided.


Landscape Pattern And Wild Bee Communities In Maine, Brianne Du Clos 2019 University of Maine

Landscape Pattern And Wild Bee Communities In Maine, Brianne Du Clos

Electronic Theses and Dissertations

Commercial production of lowbush blueberry (Vaccinium angustifolium Aiton) in Maine relies primarily on managed honeybee hives; however, naturally occurring wild bees are more efficient pollinators of the crop. Wild bees have short foraging distances and must nest near crop fields to provide pollination services. After crop bloom, the surrounding landscape must provide sufficient forage to maintain wild bee populations for the remainder of the growing season. Lowbush blueberries in Maine are produced in a mixed-use landscape with two distinct landscape contexts. Here, we document bee communities and habitat resources (nesting and floral) in power line rights-of-way and eight land cover ...


Forward Osmosis Flow Rate Differential Using Different Osmotic Agents, John M. Trzinski 2019 Embry Riddle Aeronautical University

Forward Osmosis Flow Rate Differential Using Different Osmotic Agents, John M. Trzinski

Beyond: Undergraduate Research Journal

Life support is one of the most vital systems flown on manned spaceflight missions. The systems currently used are large, heavy, inefficient, and power consuming in an environment that requires nearly perfect conditions to function. Forward Osmosis (FO) is a form of filtration that uses the natural properties of water and concentration gradients to filter water without the need for any electricity. An experiment was conducted during the Embry Riddle Summer 2018 study abroad to the LUNARES research station in Piła, Poland. This experiment explored how the flow rate of water through a FO filtration system could be affected by ...


Thiol-Based Misfolding: Linking Redox Balance To Cytosolic Proteostasis, Ford Amy 2019 The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences

Thiol-Based Misfolding: Linking Redox Balance To Cytosolic Proteostasis, Ford Amy

The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences Dissertations and Theses (Open Access)

The eukaryotic cytosolic proteome is vulnerable to changes in proteostatic and redox balance caused by temperature, pH, oxidants and xenobiotics. Cysteine-containing proteins are especially at risk as the thiol side chain is subject to oxidation, adduction and chelation by thiol-reactive compounds. All of these thiol-modifiers have been demonstrated to induce the heat shock response and recruit protein chaperones to sites of presumed protein aggregation in the budding yeast Saccharomyces cerevisiae. However, endogenous targets of thiol stress toxicity responsible for these outcomes are largely unknown. Furthermore, I hypothesize proteins identified as redox-active are prone to misfolding and aggregation by thiol-specific stress ...


Identification Of Anaerobes In Clinical Specimens Comparison Between The Rapid Ana Ii System And The Bruker Maldi-Tof Ms System Utilized In The Clinical Laboratory, Raymond Chow 2019 Dominican University of California

Identification Of Anaerobes In Clinical Specimens Comparison Between The Rapid Ana Ii System And The Bruker Maldi-Tof Ms System Utilized In The Clinical Laboratory, Raymond Chow

Natural Sciences and Mathematics | Clinical Laboratory Sciences Culminating Projects

Matrix-Assisted Laser Desorption and Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has been revealed as an invaluable platform for identifying anaerobic bacteria in the clinical laboratory over traditional methods such as the RapID ANA II System.

A qualitative comparison is made, through the analysis of methodologies and specifications, to determine whether the RapID ANA II system or Bruker MALDI-TOF MS is more suitable for identifying anaerobic organism in the clinical laboratory. Based on the data reviewed, the MALDI-TOF MS is a more intuitive platform within the clinical laboratory due to its increased specificity, cost-effectiveness, and shorten turnaround time for the identification ...


Detection Of Genetic Thrombophilia (Fa V Leiden & Pt20210 Mutation) Using Genexpert Technology With Iqcp Implementation, Mariegrace Saquilayan 2019 Dominican University of California

Detection Of Genetic Thrombophilia (Fa V Leiden & Pt20210 Mutation) Using Genexpert Technology With Iqcp Implementation, Mariegrace Saquilayan

Natural Sciences and Mathematics | Clinical Laboratory Sciences Culminating Projects

Abstract

Individuals having Genetic Thrombophilia pose a higher risk of having a thrombotic event. It is crucial to determine these gene variants to prevent a possible episode of thromboembolism. With the current PCR method, it involves individual processing of DNA isolation, amplification, and detection using three (3) different instruments resulting to an increased turnaround time of 5 to 7 days and additional staff utilization. It is performed by repetitive manual sample pipetting and preparation of reagent master mixes in small vials. Results interpretations are entered manually to a worklist built initially for final verification. These processes increase the risk of ...


Rejuvenation Of The Epigenetic Landscape Of The Aged Brain Through Manipulation Of Circulating Factors, Edward Koellhoffer 2019 The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences

Rejuvenation Of The Epigenetic Landscape Of The Aged Brain Through Manipulation Of Circulating Factors, Edward Koellhoffer

The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences Dissertations and Theses (Open Access)

The aging population of the United States is expanding at an alarming rate. The Center for Disease Control and Prevention estimates that the population of those age 65 years and older will reach over 50 million by 2020 and will double to 100 million by 2060. This will not only put a massive strain on national healthcare resources, but will also increase the number of those who are not able to live and function independently. It is becoming increasingly vital to understand how the brain changes with age and mechanisms to possibly protect and rejuvenate the aged brain to a ...


The Effects Of Salinity On Zootechnical Performance Of Spotted Seatrout (Cynoscion Nebulosus) In Recirculating Aquaculture Systems, Eric Gigli 2019 University of Southern Mississippi

The Effects Of Salinity On Zootechnical Performance Of Spotted Seatrout (Cynoscion Nebulosus) In Recirculating Aquaculture Systems, Eric Gigli

Master's Theses

The spotted seatrout (Cynoscion nebulosus) is a euryhaline finfish candidate for marine aquaculture in the northern Gulf of Mexico. Aquaculture of this species currently uses salinities of 25 psu or greater making production challenging in areas where high salinity water is not available. This study aimed to document the effects of salinity on zootechnical performance of spotted seatrout embryos, early larvae, and juveniles in recirculating aquaculture systems to assist with the development of protocols for low salinity culture.

Embryos obtained from a volitional spawn at 29 psu were incubated at three salinities (12.5, 18.75, or 25 psu) until ...


Acute Promyelocytic Leukemia: A Case Study Correlating Cytogenetic Abnormality With Prognosis, Alyssa Lamond, Theresa Tellier-Castellone 2019 University of Rhode Island

Acute Promyelocytic Leukemia: A Case Study Correlating Cytogenetic Abnormality With Prognosis, Alyssa Lamond, Theresa Tellier-Castellone

Senior Honors Projects

Advancements in medical technology today have positively impacted the diagnosis, treatment, and prognosis of cancers. Particularly acute promyelocytic leukemia (APL) has completely improved from having the poorest prognosis to one of the best. Acute promyelocytic leukemia is a malignant disease of hematopoietic tissue classified by WHO as leukemia with >20% blasts from the myeloid lineage, specifically promyelocytes. Determined in 1976, FAB classified AML subtypes M1-M7, with APL being M3. Specific characteristics classify the subtype of AML, with each resulting from a different genetic abnormality. The focus of APL diagnosis, treatment, and prognosis occurs around the known PML-RARα fusion gene. Flow ...


The Relationship Between Acute Exposure To Hypoxia And Heart Rate Variability In Unacclimatized Individuals, Grace Becker 2019 William & Mary

The Relationship Between Acute Exposure To Hypoxia And Heart Rate Variability In Unacclimatized Individuals, Grace Becker

Undergraduate Honors Theses

Acute exposure to hypoxia results in a stress response categorized by sympathetic dominance, including hyperventilation to prevent arterial desaturation. As an estimation of overall stress, heart rate variability (HRV) reflects the balance between sympathetic and parasympathetic autonomic regulation. PURPOSE: The purpose of this study was to determine whether resting sea level (SL) HRV is correlated with arterial desaturation and respiratory responses to acute normobaric hypoxia exposure (equivalent 3500 m). METHODS: Resting HRV, %SpO2, and respiratory rate (RR) were measured in 19 subjects (male n=9, female n=10) at SL for 15 minutes. HRV was measured (Firstbeat Bodyguard2) including ...


Animal Experimentation: Working Towards A Paradigm Change, Kathrin Herrmann (ed.), Kimberley Jayne (ed.) 2019 Johns Hopkins Bloomberg School of Public Health

Animal Experimentation: Working Towards A Paradigm Change, Kathrin Herrmann (Ed.), Kimberley Jayne (Ed.)

eBooks

Animal experimentation has been one of the most controversial areas of animal use, mainly due to the intentional harms inflicted upon animals for the sake of hoped-for benefits in humans. Despite this rationale for continued animal experimentation, shortcomings of this practice have become increasingly more apparent and well-documented. However, these limitations are not yet widely known or appreciated, and there is a danger that they may simply be ignored. The 51 experts who have contributed to Animal Experimentation: Working Towards a Paradigm Change critically review current animal use in science, present new and innovative non-animal approaches to address urgent scientific ...


Potential Antimicrobial Activity Of Plants In The Genus Plantago, McKenna Hunt 2019 University of North Alabama

Potential Antimicrobial Activity Of Plants In The Genus Plantago, Mckenna Hunt

Scholars Week 2019

The aim of this study was to investigate potential antimicrobial activity of phytochemical extracts of commercially available species of Plantago. Plantago is a medically relevant genus, with several species traditionally used worldwide to treat inflammation and various infections. Dried leaf material of both species was extracted using a Soxhlet apparatus. Nonpolar (hexane) extracts were obtained from both P. subnuda and P. eriopoda, as well as a polar (methanol) extract from P. eriopoda. Minimum inhibitory concentration (MIC) assays were performed using 96-well microtiter plates against seven different bacterial species (Escherichia coli, Bacillus megaterium, Klebsiella pneumoniae, Mycobacterium smegmatis, Pseudomonas aeruginosa, Serratia marcescens ...


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