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Federal Technology Transfer: Should We Build Subarus In Bethesda, Christopher J. Harnett 2016 University of New Hampshire

Federal Technology Transfer: Should We Build Subarus In Bethesda, Christopher J. Harnett

RISK: Health, Safety & Environment

A critical examination of a policy designed to encourage commercial exploitation of federally funded biomedical research. The author argues that the implementation of this policy threatens the integrity of basic science in America.


Abe Fermentation From Low Cost Substrates, Kai Gao 2016 The University of Western Ontario

Abe Fermentation From Low Cost Substrates, Kai Gao

Electronic Thesis and Dissertation Repository

The high cost of substrate and product inhibition in the fermentation broth remains two major problems associated with bio-butanol production. This thesis aims to solve these problems by examining abundant lignocellulosic biomass as potential feedstocks and exploring novel substrates such as carbohydrates derived from microalgae for ABE fermentation. The commonly observed toxic effect after pretreatment of lignocelluslosic biomass was removed by resin adsorption, where the resin could also serve as an in-situ butanol recvoery devices.

Corn cobs (an agricultural waste), switchgrass (an energy crop) and phragmites (an invasive plant in North America) were investigated as substrates for ABE fermentation by ...


Structural Determinants Allowing Transferase Activity In Sensitive To Freezing 2, Classified As A Family I Glycosyl Hydrolase, Rebecca Roston, Kun Wang, Leslie A. Kuhn, Christoph Benning 2016 University of Nebraska- Lincoln

Structural Determinants Allowing Transferase Activity In Sensitive To Freezing 2, Classified As A Family I Glycosyl Hydrolase, Rebecca Roston, Kun Wang, Leslie A. Kuhn, Christoph Benning

Biochemistry -- Faculty Publications

Background: SENSITIVE TO FREEZING 2 (SFR2) is classified as a glycosyl hydrolase, and by using glycosyltransferase activity, it modifies membrane lipids to promote freeze tolerance.

Results: Although the active site of SFR2 is identical to hydrolases, adjacent loop regions contribute to its transferase activity.

Conclusion: Transferase activity evolved by modifications external to the core catalytic site.

Significance: Defined structure-function relationships will inform engineering of transferases and freeze tolerance.


Optimizing Gdna Purification And Qpcr Techniques For Specific Detection And Enumeration Of Probiotic Bacteria, Katelynn C. Lee, Jennifer VanderKelen, Christopher Kitts 2016 California Polytechnic State University, San Luis Obispo

Optimizing Gdna Purification And Qpcr Techniques For Specific Detection And Enumeration Of Probiotic Bacteria, Katelynn C. Lee, Jennifer Vanderkelen, Christopher Kitts

STAR (STEM Teacher and Researcher) Program Posters

Probiotics are live microorganisms, like bacteria and yeast, which are thought to provide health benefits when ingested. Large manufacturers produce blends of probiotics for supplementing the diets of agriculturally important animals. Commercial probiotic products are labeled with the number of cells present at the time of manufacture. Our assignment was to develop an assay which can be used to quantify the number of specific lactic acid bacteria present in certain probiotic products, thus verifying the number reported on the product labels. This quantification process involves isolating genomic DNA (gDNA) from the given samples and then running the DNA through a ...


Contribution Of A Putative Up Element Dna Sequence To The Activity Of A Newly Identified Phage Promoter, Courtney Hamilton 2016 Western Kentucky University

Contribution Of A Putative Up Element Dna Sequence To The Activity Of A Newly Identified Phage Promoter, Courtney Hamilton

Honors College Capstone Experience/Thesis Projects

In transcription, a universal step in gene expression, information from a DNA sequence is copied into RNA. A key component in gene expression is the promoter sequence, a region of DNA to which RNA polymerase binds during the initiation of transcription of downstream genes. Most bacterial promoters contain a -10 and a -35 sequence that are bound by the RNA polymerase. Some promoters also contain an Upstream Promoter (UP) element. UP elements have been shown to boost promoter activity. We recently identified a new promoter in a mutant bacteriophage that grows on a bacterial host that prevents antitermination of phage ...


The Effect Of An Enhanced Isopentenyl Monophosphate Pool On Terpenoid Biosynthesis In Vivo, Evan T. Adams, Laura K. Henry, Dr. Natalia Dudareva 2016 Department of Biochemistry, Purdue University

The Effect Of An Enhanced Isopentenyl Monophosphate Pool On Terpenoid Biosynthesis In Vivo, Evan T. Adams, Laura K. Henry, Dr. Natalia Dudareva

The Summer Undergraduate Research Fellowship (SURF) Symposium

Found in all living organisms, terpenoids make up the largest group of natural products and are essential compounds for many major processes, including photosynthesis, respiration, hormone production, and electron transport. Additionally, they have commercial and medical value in products including fragrances, cosmetics, and medicines. Terpenoids originate from the five-carbon building blocks isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), which are synthesized by the mevalonic acid (MVA) and methylerithritol phosphate (MEP) pathways. An alternative MVA pathway was discovered in Archaea with the final two enzymes being phosphomevalonate decarboxylase (MPD) and isopentenyl phosphate kinase (IPK). Even though this alternative pathway is not ...


Design And Development Of A Plasmid Vector For Protein Expression And Purification, Mahima Grover, Craig Sweet, David H. Thompson 2016 Purdue University

Design And Development Of A Plasmid Vector For Protein Expression And Purification, Mahima Grover, Craig Sweet, David H. Thompson

The Summer Undergraduate Research Fellowship (SURF) Symposium

Production and isolation of proteins are difficult, costly and time-consuming processes. The aim of this project is for the development of plasmids, which allow for streamlined production and isolation of proteins. To allow for modular insertion of varying segments of DNA we are using ‘recursive directional ligation by plasmid reconstruction’. This technique uses type II restriction endonucleases, which cut downstream from their recognition site allowing multiple insertions without losing a restriction site. Using this process, we can ligate multiple DNA sequences together and express them to be able to construct a scar less fusion protein. In order to accomplish this ...


The Evolution Of The Bacterial Luciferase Gene Cassette (Lux) As A Real-Time Bioreporter, Dan Close, Tingting Xu, Abby Smartt, Alexandra Rogers, Robert Crossley, Sarah Price, Steven Ripp, Gary Sayler 2016 University of Tennessee - Knoxville

The Evolution Of The Bacterial Luciferase Gene Cassette (Lux) As A Real-Time Bioreporter, Dan Close, Tingting Xu, Abby Smartt, Alexandra Rogers, Robert Crossley, Sarah Price, Steven Ripp, Gary Sayler

Steven Ripp

The bacterial luciferase gene cassette (lux) is unique among bioluminescent bioreporter systems due to its ability to synthesize and/or scavenge all of the substrate compounds required for its production of light. As a result, the lux system has the unique ability to autonomously produce a luminescent signal, either continuously or in response to the presence of a specific trigger, across a wide array of organismal hosts. While originally employed extensively as a bacterial bioreporter system for the detection of specific chemical signals in environmental samples, the use of lux as a bioreporter technology has continuously expanded over the last ...


Comparison Of Human Optimized Bacterial Luciferase, Firefly Luciferase, And Green Fluorescent Protein For Continuous Imaging Of Cell Culture And Animal Models, Dan M. Close, Ruth E. Hahn, Stacey S. Patterson, Seung J. Baek, Steven Ripp, Gary S. Sayler 2016 University of Tennessee - Knoxville

Comparison Of Human Optimized Bacterial Luciferase, Firefly Luciferase, And Green Fluorescent Protein For Continuous Imaging Of Cell Culture And Animal Models, Dan M. Close, Ruth E. Hahn, Stacey S. Patterson, Seung J. Baek, Steven Ripp, Gary S. Sayler

Steven Ripp

Bioluminescent and fluorescent reporter systems have enabled the rapid and continued growth of the optical imaging field over the last two decades. Of particular interest has been noninvasive signal detection from mammalian tissues under both cell culture and whole animal settings. Here we report on the advantages and limitations of imaging using a recently introduced bacterial luciferase (lux) reporter system engineered for increased bioluminescent expression in the mammalian cellular environment. Comparison with the bioluminescent firefly luciferase (Luc) system and green fluorescent protein system under cell culture conditions demonstrated a reduced average radiance, but maintained a more constant level of bioluminescent ...


In Vivo Bioluminescent Imaging (Bli): Noninvasive Visualization And Interrogation Of Biological Processes In Living Animals, Dan M. Close, Tingting Xu, Gary S. Sayler, Steven Ripp 2016 University of Tennessee - Knoxville

In Vivo Bioluminescent Imaging (Bli): Noninvasive Visualization And Interrogation Of Biological Processes In Living Animals, Dan M. Close, Tingting Xu, Gary S. Sayler, Steven Ripp

Steven Ripp

In vivo bioluminescent imaging (BLI) is increasingly being utilized as a method for modern biological research. This process, which involves the noninvasive interrogation of living animals using light emitted from luciferase-expressing bioreporter cells, has been applied to study a wide range of biomolecular functions such as gene function, drug discovery and development, cellular trafficking, protein-protein interactions, and especially tumorigenesis, cancer treatment, and disease progression. This article will review the various bioreporter/biosensor integrations of BLI and discuss how BLI is being applied towards a new visual understanding of biological processes within the living organism.

doi:10.3390/s110100180


The Evolution Of The Bacterial Luciferase Gene Cassette (Lux) As A Real-Time Bioreporter, Dan Close, Tingting Xu, Abby Smartt, Alexandra Rogers, Robert Crossley, Sarah Price, Steven Ripp, Gary Sayler 2016 University of Tennessee - Knoxville

The Evolution Of The Bacterial Luciferase Gene Cassette (Lux) As A Real-Time Bioreporter, Dan Close, Tingting Xu, Abby Smartt, Alexandra Rogers, Robert Crossley, Sarah Price, Steven Ripp, Gary Sayler

Steven Ripp

The bacterial luciferase gene cassette (lux) is unique among bioluminescent bioreporter systems due to its ability to synthesize and/or scavenge all of the substrate compounds required for its production of light. As a result, the lux system has the unique ability to autonomously produce a luminescent signal, either continuously or in response to the presence of a specific trigger, across a wide array of organismal hosts. While originally employed extensively as a bacterial bioreporter system for the detection of specific chemical signals in environmental samples, the use of lux as a bioreporter technology has continuously expanded over the last ...


Comparison Of Human Optimized Bacterial Luciferase, Firefly Luciferase, And Green Fluorescent Protein For Continuous Imaging Of Cell Culture And Animal Models, Dan M. Close, Ruth E. Hahn, Stacey S. Patterson, Seung J. Baek, Steven Ripp, Gary S. Sayler 2016 University of Tennessee - Knoxville

Comparison Of Human Optimized Bacterial Luciferase, Firefly Luciferase, And Green Fluorescent Protein For Continuous Imaging Of Cell Culture And Animal Models, Dan M. Close, Ruth E. Hahn, Stacey S. Patterson, Seung J. Baek, Steven Ripp, Gary S. Sayler

Steven Ripp

Bioluminescent and fluorescent reporter systems have enabled the rapid and continued growth of the optical imaging field over the last two decades. Of particular interest has been noninvasive signal detection from mammalian tissues under both cell culture and whole animal settings. Here we report on the advantages and limitations of imaging using a recently introduced bacterial luciferase (lux) reporter system engineered for increased bioluminescent expression in the mammalian cellular environment. Comparison with the bioluminescent firefly luciferase (Luc) system and green fluorescent protein system under cell culture conditions demonstrated a reduced average radiance, but maintained a more constant level of bioluminescent ...


In Vivo Bioluminescent Imaging (Bli): Noninvasive Visualization And Interrogation Of Biological Processes In Living Animals, Dan M. Close, Tingting Xu, Gary S. Sayler, Steven Ripp 2016 University of Tennessee - Knoxville

In Vivo Bioluminescent Imaging (Bli): Noninvasive Visualization And Interrogation Of Biological Processes In Living Animals, Dan M. Close, Tingting Xu, Gary S. Sayler, Steven Ripp

Steven Ripp

In vivo bioluminescent imaging (BLI) is increasingly being utilized as a method for modern biological research. This process, which involves the noninvasive interrogation of living animals using light emitted from luciferase-expressing bioreporter cells, has been applied to study a wide range of biomolecular functions such as gene function, drug discovery and development, cellular trafficking, protein-protein interactions, and especially tumorigenesis, cancer treatment, and disease progression. This article will review the various bioreporter/biosensor integrations of BLI and discuss how BLI is being applied towards a new visual understanding of biological processes within the living organism.

doi:10.3390/s110100180


Nanocomposite Adhesive Of English Ivy (Hedera Helix): Bioproduction, Nanoparticle Isolation, And Molecular Analysis, Jason Neil Burris 2016 University of Tennessee, Knoxville

Nanocomposite Adhesive Of English Ivy (Hedera Helix): Bioproduction, Nanoparticle Isolation, And Molecular Analysis, Jason Neil Burris

Doctoral Dissertations

English ivy naturally produces organic nanoparticles from its adventitious root hairs, and possesses characteristics that may allow them to replace metal-based nanoparticles in common applications, such as sunscreen. At the onset of the research, it was hypothesized that a physical mechanism of attachment, similar to the gecko footpad, was used to generate the adhesive force for attachment; however, through the results obtained from recent work, it is clear that a biochemical mechanism is involved in the generation of the strength of adhesion. Therefore, the goal of this research was to provide a better understanding of the genetic basis of nanoparticle ...


Optimization Of A Genomic Editing System Using Crispr/Cas9-Induced Site-Specific Gene Integration, Jillian L. McCool Ms., Nick Hum, Gabriela G. Loots 2016 California State University, Chico

Optimization Of A Genomic Editing System Using Crispr/Cas9-Induced Site-Specific Gene Integration, Jillian L. Mccool Ms., Nick Hum, Gabriela G. Loots

STAR (STEM Teacher and Researcher) Program Posters

The CRISPR-Cas system is an adaptive immune system found in bacteria which helps protect against the invasion of other microorganisms. This system induces double stranded breaks at precise genomic loci (1) in which repairs are initiated and insertions of a target are completed in the process. This mechanism can be used in eukaryotic cells in combination with sgRNAs (1) as a tool for genome editing. By using this CRISPR-Cas system, in addition to the “safe harbor locus,” ROSAβ26, the incorporation of a target gene into a site that is not susceptible to gene silencing effects can be achieved through few ...


Fermentative Processes Requiring Low Solubility Feed Gases: An Investigation Into Gas-Dependent Microorganisms, Eric W. Doerr 2016 The University of Western Ontario

Fermentative Processes Requiring Low Solubility Feed Gases: An Investigation Into Gas-Dependent Microorganisms, Eric W. Doerr

Electronic Thesis and Dissertation Repository

Two bioprocesses were separately investigated based on their common interest of using gaseous substrates that have low solubility. The first process involved the development of a mixed culture using two organisms capable of utilizing glycerol and carbon monoxide separately to increase biobutanol production, while the second process involved an investigation of different production media used in aerobic xanthan production of Xanthomonas campestris with pressurization effects. It was determined that Clostridium pasteurianum should be used with an organism like Clostridium carboxidivorans or Eubacterium limosum in order for butyrate uptake at 3 g/L to occur with a minimum 0.1 g ...


Microbubble Generation By Piezoelectric Transducers For Biomedical Studies, Mohammed Alkhazal 2016 Old Dominion University

Microbubble Generation By Piezoelectric Transducers For Biomedical Studies, Mohammed Alkhazal

Engineering Technology Theses & Dissertations

Bubbles induced by blast waves or shocks are speculated as the major cause of damage in biological cells in mild traumatic brain injuries (TBI). Microbubble collapse was found to induce noticeable cell detachment from the cell substrate, changes in focal adhesion, and biomechanics. To better understand the bubble mechanism, a system needs to be constructed which allows clear differentiation on the impact of bubbles from that of shocks. Such a generator needs to be low profile in order to place under a microscope. A piezoelectric transducer system was designed to meet the need. The system uses either a flat or ...


Membrane Disruption Antimicrobial Mechanism Of Channa Striatus Lysozyme-Derived Antimicrobial Peptide, Venkatesh Kumaresan 2016 SRM University

Membrane Disruption Antimicrobial Mechanism Of Channa Striatus Lysozyme-Derived Antimicrobial Peptide, Venkatesh Kumaresan

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


Channa Striatus Tnfr-1: Molecular Cloning, Characterization And Gene Expression, Rajesh Palanisamy 2016 SRM Institute of Science and Technology

Channa Striatus Tnfr-1: Molecular Cloning, Characterization And Gene Expression, Rajesh Palanisamy

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


A Comparative Statement On Molecular Approach Of Large Hsps From Macrobrachium Rosenbergii, Mukesh Kumar Chaurasia 2016 SRM UNIVERSITY

A Comparative Statement On Molecular Approach Of Large Hsps From Macrobrachium Rosenbergii, Mukesh Kumar Chaurasia

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


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