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Affluent Populations And Their Effect On Biological Diversity Through The Consumption Of Meat, Electronics, And Motor Vehicles, Melody Flores 2014 Pace University

Affluent Populations And Their Effect On Biological Diversity Through The Consumption Of Meat, Electronics, And Motor Vehicles, Melody Flores

Honors College Theses

The human has caused a far greater impact on the planet's biodiversity than any other species in existence, due to the impact of population, afflluence, and technology. This thesis will argue the importance of biological diversity and how affluent populations are reducing biodiversity through the consumption of meat, electronics, and motor vehicles. Aldo Leopold's "The Land Ethic" and Herman Daly's "The Impossibility Theorem", among others, create a rubric evaluating human activities and provide alternative views on economic impossibilities. Consumption is reviewed from an ecocentric perspective, a holistic outlook placing emphasis on the ecosystem. The reader will become ...


Tattletales And T-Bow Update 20140602mon, George McNamara 2014 The University of Texas

Tattletales And T-Bow Update 20140602mon, George Mcnamara

George McNamara

Tattletales and T-Bow Update 20140602Mon

http://works.bepress.com/gmcnamara/42

Please see also http://works.bepress.com/gmcnamara/26

Tattletales: multiplex fluorescent protein biosensors by spatial localization with TALE-FPs, Cas9-FPs, ZF-FPs, LacI-FPs, TetR-FPs, etc.

T-Bow: Rainbow T-cells and Tumor cells (and ES cells, iPS cells, other cells and organisms). You can think of this as "Brainbow meets TALENs/Cas9/ZFNs/other DNA sequence specific binding proteins".

If not familiar with Brainbow, see

http://en.wikipedia.org/wiki/Brainbow

If not familiar with TALENs, Cas9, etc, see

http://www.addgene.org/genome_engineering/

Big idea: localizing fluorescent proteins - and/or Nano-Lanterns ...


Water Water Everywhere: Analyzing Long Island's Water Issues And Finding Solutions For A Sustainable Future, Anthony T. Becker 2014 Fordham University

Water Water Everywhere: Analyzing Long Island's Water Issues And Finding Solutions For A Sustainable Future, Anthony T. Becker

2014 Student Theses

Over three million people call Long Island their home. With access to beautiful landscapes, world-renowned beaches, and proximity to New York City, it is no wonder that so many proudly call this geographic stretch of glacial till their home. However, throughout the years our actions do not necessarily reflect this affection we have to our home. Years of sprawl and human infestation across the island have resulted in widespread environmental degradation. Specifically, the water we drink and the beaches we enjoy have become endangered. I plan on studying the urban ecology of how intensified population growth led to the eutrophication ...


Characterization Of Jumonji-Domain Containing Proteins In Arabidopsis Thaliana, Jennifer Lawson 2014 Syracuse University

Characterization Of Jumonji-Domain Containing Proteins In Arabidopsis Thaliana, Jennifer Lawson

Syracuse University Honors Program Capstone Projects

Jumonji-C (JmjC) domain containing proteins have been shown to play a critical role in regulating gene expression by regulating chromatin remodeling in a variety of organisms. In Arabidopsis, JmjC protein family consists of 21 members, some of which have been shown to be involved in regulating histone demethylation. Most of these proteins have been characterized for their role in regulating plant development, especially for their role in regulating flowering time. Here we report characterization of three JmjC domain containing proteins - JUMONJI1, JUMONJI2, and JUMONJI27 and show that they play a critical role in regulating both pathogen defense and flowering time ...


Evaluation Of Novel Multi-Dimensional Tissue Culturing Methods Using Autonomously Bioluminescent Human Cell Lines, James Dean Webb 2014 University of Tennessee, Knoxville

Evaluation Of Novel Multi-Dimensional Tissue Culturing Methods Using Autonomously Bioluminescent Human Cell Lines, James Dean Webb

University of Tennessee Honors Thesis Projects

No abstract provided.


Effects Of Continuous Combined Oral Contraceptives On Mouse Mammary Gland Structure And Tm2h Mammary Tumor Progression, Evelyn Esteves-Natal 2014 The College at Brockport: State University of New York

Effects Of Continuous Combined Oral Contraceptives On Mouse Mammary Gland Structure And Tm2h Mammary Tumor Progression, Evelyn Esteves-Natal

2014 Master's Level Graduate Research Conference

Cyclic oral contraceptive (OC) use has been associated with increased risk of breast cancer, particularly in African American women, current OC users, and women who begin use early in life. In contrast, the effect of continuous (extended) OC on breast cancer risk is unknown. The purpose of this study was to test the hypothesis that continuous and cyclic OC differ in their effects on mammary gland structure and susceptibility to tumor growth. Starting at day 50 of age, BALB/C mice were fed liquid diets +/- human equivalent doses of ethinyl estradiol and levonorgestrel. After 28 days, 10 mice per group ...


Genetic Engineering Of Yeast-Based Bioluminescent Reporters For Testing The Effect Of Human Estrogen Receptor Alpha Mutations On Its Transcriptional Activity, Peter Bowen Hjorth 2014 University of Tennessee, Knoxville

Genetic Engineering Of Yeast-Based Bioluminescent Reporters For Testing The Effect Of Human Estrogen Receptor Alpha Mutations On Its Transcriptional Activity, Peter Bowen Hjorth

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Exhibition of Undergraduate Research and Creative Achievement (EURēCA)


The Role Of Sirtuin 1 And Ceramide In T10C12 Conjugated Linoleic Acid Induced Delipidation In 3t3-L1 Adipocytes, Wei Wang 2014 University of Nebraska - Lincoln

The Role Of Sirtuin 1 And Ceramide In T10C12 Conjugated Linoleic Acid Induced Delipidation In 3t3-L1 Adipocytes, Wei Wang

Theses and Dissertations in Animal Science

Project 1: Trans-10, cis-12 conjugated linoleic acid (t10c12 CLA) reduces triglyceride (TG) levels in adipocytes through multiple pathways, with AMP-activated protein kinase (AMPK) generally facilitating, and peroxisome proliferator-activated receptor γ (PPARγ) generally opposing these reductions. Sirtuin 1 (SIRT1), a histone/protein deacetylase that affects energy homeostasis, often functions coordinately with AMPK, and is capable of binding to PPARγ, thereby inhibiting its activity. This study investigated the role of SIRT1 in the response of 3T3-L1 adipocytes to t10c12 CLA by testing the following hypotheses: 1) SIRT1 is functionally required for robust TG reduction ...


Issue Brief: Asset Management For Stormwater, New England Environmental Finance Center, Sustainable Communities Learning Network 2014 University of Southern Maine

Issue Brief: Asset Management For Stormwater, New England Environmental Finance Center, Sustainable Communities Learning Network

Sustainable Communities Capacity Building

Asset management is a strategic approach to maintaining and sustaining infrastructure in order to meet the needs of the community at the lowest overall life cycle cost. This approach helps communities know how and where to prioritize limited funds in order to achieve the greatest benefit. Often applied to drinking water and wastewater infrastructure, this method is well suited to managing any assets, including stormwater systems.

This issue brief is intended to introduce local governments to the asset management process and to show how it can be applied in managing stormwater assets. It was adapted from an appendix written by ...


General Mills Turns To 'Raja Of The Country Of World Peace' To Certify Its Costlier, Non-Gmo Cheerios, Drew L. Kershen 2014 University of Oklahoma College of Law

General Mills Turns To 'Raja Of The Country Of World Peace' To Certify Its Costlier, Non-Gmo Cheerios, Drew L. Kershen

Drew L. Kershen

No abstract provided.


Using Progesterone As An Indicator Of Ovarian Response To Stimulation In Cattle, Kayla Mariah Kauffman 2014 California Polytechnic State University

Using Progesterone As An Indicator Of Ovarian Response To Stimulation In Cattle, Kayla Mariah Kauffman

Animal Science

No abstract provided.


Zyflamend, A Polyherbal Mixture, Down Regulates Class I And Class Ii Histone Deacetylases And Increases P21 Levels In Castrate-Resistant Prostate Cancer Cells, E-Chu Huang, Yi Zhao, Guoxun Chen, Seung Joon Baek, Michael F. McEntee, Steven Minkin, John P. Biggerstaff, Jay Whelan 2014 University of Tennessee, Knoxville

Zyflamend, A Polyherbal Mixture, Down Regulates Class I And Class Ii Histone Deacetylases And Increases P21 Levels In Castrate-Resistant Prostate Cancer Cells, E-Chu Huang, Yi Zhao, Guoxun Chen, Seung Joon Baek, Michael F. Mcentee, Steven Minkin, John P. Biggerstaff, Jay Whelan

Nutrition Publications and Other Works

Background

Zyflamend, a mixture containing extracts of ten herbs, has shown promise in a variety of preclinical cancer models, including prostate cancer. The current experiments were designed to investigate the effects of Zyflamend on the expression of class I and II histone deacetylases, a family of enzymes known to be over expressed in a variety of cancers.

Methods

CWR22Rv1 cells, a castrate-resistant prostate cancer cell line, were treated with Zyflamend and the expression of class I and II histone deacetylases, along with their downstream target the tumor suppressor gene p21, was investigated. Involvement of p21 was confirmed with siRNA knockdown ...


Arizona Clinical Research Workforce Survey, Cris E. Wells, Julie A. Robbins, Gaye Luna 2014 Southern Illinois University Carbondale

Arizona Clinical Research Workforce Survey, Cris E. Wells, Julie A. Robbins, Gaye Luna

Online Journal for Workforce Education and Development

Objective: We characterize the current clinical research workforce in Arizona with the goal of identifying possible educational training gaps for the future workforce. Background: The science community, government entities, educational institutions, and community programs continue to advocate for additional funding and resources to educate the bioscience workforce, of which clinical research is a part. However, there is little evidence that additional training and/or education is needed within the clinical research profession. Method: We utilized an electronic survey tool that was designed to solicit workforce data from clinical research professionals actively working or seeking employment in the field of clinical ...


Time Series Data For 3b Image Processing, George McNamara 2014 The University of Texas

Time Series Data For 3b Image Processing, George Mcnamara

George McNamara

Time series data for 3B image processing

Four time series data sets of Stellaris single molecules RNA FISH (fluorescence in situ hybridization). All four datasets are 301 imaqe planes. FISH probes are to TOP1 mRNA (Topoisomerase I)in Saos osterosarcoma cells.

One dataset is 10 millisecond exposures (very dim), acquired in Streaming acquisition mode (no hardware overhead).

The other three datasets are a three consecutive planes of the same XY field of view.

I have included image processing results for:

PiMP - a fast method developed by Sebastian Munck et al 2012, http://www.ncbi.nlm.nih.gov/pubmed/?term=22357945 ...


Understanding The Chemical Gymnastics Of Enzyme-Catalyzed 1’-1 And 1’-3 Triterpene Linkages, Stephen A. Bell 2014 University of Kentucky

Understanding The Chemical Gymnastics Of Enzyme-Catalyzed 1’-1 And 1’-3 Triterpene Linkages, Stephen A. Bell

Theses and Dissertations--Plant and Soil Sciences

Squalene synthase (SS) is an essential enzyme in eukaryotic systems responsible for an important branch point in isoprenoid metabolism that leads to sterol formation. The mechanistic complexity of SS has made it a difficult enzyme to study. The green alga Botryococcus braunii race B possesses several squalene synthase-like (SSL) enzymes that afford a unique opportunity to study the complex mechanism of triterpene biosynthesis. SSL-1 catalyzes presqualene diphosphate (PSPP) formation, which can either be converted to squalene by SSL-2 or botryococcene by SSL-3. A rationally designed mutant study of B. braunii squalene synthase (BbSS) and SSL-3 was conducted to understand structure-function ...


Systematic Screen Of Chemotherapeutics In Drosophila Stem Cell Tumors, Michele Markstein, Samantha Detorree, Julio Cho, Ralph Neumüller, Soren Craig-Müller, Norbert Perrimon 2014 University of Massachusetts - Amherst

Systematic Screen Of Chemotherapeutics In Drosophila Stem Cell Tumors, Michele Markstein, Samantha Detorree, Julio Cho, Ralph Neumüller, Soren Craig-Müller, Norbert Perrimon

Michele Markstein

Here we report the development of an in vivo system to study the interaction of stem cells with drugs using a tumor model in the adult Drosophila intestine. Strikingly, we find that some Food and Drug Administration-approved chemotherapeutics that can inhibit the growth of Drosophila tumor stem cells can paradoxically promote the hyperproliferation of their wild-type counterparts. These results reveal an unanticipated side effect on stem cells that may contribute to tumor recurrence. We propose that the same side effect may occur in humans based on our finding that it is driven in Drosophila by the evolutionarily conserved Janus kinase-signal ...


The Effects Of Salinity, Ph, Temperature, And Dissolved Oxygen On Sensitivity Of Pcr Identification Of T4 Bacteriophage, Joesph F. Cannon, Nicholas A. Thurn, Paul E. Richardson 2013 University of South Carolina

The Effects Of Salinity, Ph, Temperature, And Dissolved Oxygen On Sensitivity Of Pcr Identification Of T4 Bacteriophage, Joesph F. Cannon, Nicholas A. Thurn, Paul E. Richardson

Journal of the South Carolina Academy of Science

Bacteriophages are used as indicators of pathogenic bacteria in drinking, and wastewaters. They also show potential in limiting aquatic bacterial populations through their lytic properties. The effect of different water characteristics (salinity, pH, dissolved oxygen, and temperature) on the sensitivity of the PCR identification of virus particles were analyzed to determine at what levels bacteriophage can be detected in environmental samples. Results from this preliminary study indicate that a PCR bacteriophage detection technique has potential as a relatively efficient and economical indicator of coliform contamination in multiple aquatic environments. While further evaluation is needed, the protocol appears to function in ...


Role Of Carotenoid Cleavage Dioxygenases In Volatile Emissions And Insect Resistance In Arabidopsis, Shailu Lakshminarayan 2013 Western University

Role Of Carotenoid Cleavage Dioxygenases In Volatile Emissions And Insect Resistance In Arabidopsis, Shailu Lakshminarayan

University of Western Ontario - Electronic Thesis and Dissertation Repository

In Arabidopsis thaliana, carotenoid degradation by enzymatic oxidative cleavage is catalyzed by nine CCD and NCED enzymes; this cleavage produces an array of terpenoid products that are collectively known as apocarotenoids, which include volatile and non-volatile compounds. Previous studies on CCD1 revealed that its overexpression in A. thaliana enhances β-ionone emission and reduces damage caused by flea beetles. In consideration of these findings, I generated CCD4 and CCD8 Arabidopsis overexpression lines. Further, I characterized these overexpression lines on the basis of their carotenoid content and their volatile profiles. Surprisingly, accumulation of key carotenoids in the leaves was observed in both ...


Effects Of Biodiesel Concentration On Microbial Deterioration Of Polyethylene In A Simulated Fuel Storage Tank, Juan Manuel Restrepo-Florez 2013 Western University

Effects Of Biodiesel Concentration On Microbial Deterioration Of Polyethylene In A Simulated Fuel Storage Tank, Juan Manuel Restrepo-Florez

University of Western Ontario - Electronic Thesis and Dissertation Repository

In this study a simulated fuel storage tank was used to investigate the effect of biodiesel concentration on biodegradation of polyethylene. This research is relevant in the field of fuel storage. The simulated storage system consisted of a number of identical conical flasks. Each flask was comprised of two layers, an upper one consisting of a fuel blend of diesel with biodiesel in concentrations ranging from 0 to 100% of biodiesel and the bottom layer containing an aqueous mineral media inoculated with a community obtained from a real fuel storage facility. Polyethylene slabs cut to a specific size were immersed ...


Near-Infrared Surface-Enhanced Fluorescence Using Silver Nanoparticles In Solution, Michael D. Furtaw 2013 University of Nebraska - Lincoln

Near-Infrared Surface-Enhanced Fluorescence Using Silver Nanoparticles In Solution, Michael D. Furtaw

Mechanical (and Materials) Engineering -- Dissertations, Theses, and Student Research

Fluorescence spectroscopy is a widely used detection technology in many research and clinical assays. Further improvement to assay sensitivity may enable earlier diagnosis of disease, novel biomarker discovery, and ultimately, improved outcomes of clinical care along with reduction in costs. Near-infrared, surface-enhanced fluorescence (NIR-SEF) is a promising approach to improve assay sensitivity via simultaneous increase in signal with a reduction in background. This dissertation describes research conducted with the overall goal to determine the extent to which fluorescence in solution may be enhanced by altering specific variables involved in the formation of plasmonactive nanostructures of dye-labeled protein and silver nanoparticles ...


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