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Total Mercury Determination In Muscle And Liver Tissue Samples From Brazilian Amazon Fish Using Slurry Sampling, João Vitor de Queiroz, José Cavalcante Souza Vieira, Izabela da Cunha Bataglioli, Alis Correia Bittarello, Camila Pereira Braga, Grasieli de Oliveira, Cilene do Carmo Federici Padilha, Pedro de Magalhães Padilha 2018 São Paulo State University

Total Mercury Determination In Muscle And Liver Tissue Samples From Brazilian Amazon Fish Using Slurry Sampling, João Vitor De Queiroz, José Cavalcante Souza Vieira, Izabela Da Cunha Bataglioli, Alis Correia Bittarello, Camila Pereira Braga, Grasieli De Oliveira, Cilene Do Carmo Federici Padilha, Pedro De Magalhães Padilha

Biochemistry -- Faculty Publications

This paper presents a slurry sampling method for total mercury determination by graphite furnace atomic absorption spectrometry (GFAAS) in tissue of fish from the Amazon. The tissue samples were lyophilized and macerated, and then the slurry samples were prepared by putting 20 mg of tissue, added to a solution containing Triton X-100, Suprapur HNO3, and zirconium nitrate directly in sampling vials of a spectrometer. Mercury standard solutions were prepared under the same conditions as the slurry samples. The slurry samples and the mercury standard solutions were sonicated for 20 s. Twenty microliters of slurry samples were injected into the ...


Mrub_1283, Mrub_1284 And Mrub_1285 Encode For A Glycine/Betaine Abc Transporter And Are Orthologs Of E. Coli Prov, Prow And Prox, Lan Dang, Dr. Lori Scott 2018 Augustana College, Rock Island Illinois

Mrub_1283, Mrub_1284 And Mrub_1285 Encode For A Glycine/Betaine Abc Transporter And Are Orthologs Of E. Coli Prov, Prow And Prox, Lan Dang, Dr. Lori Scott

Meiothermus ruber Genome Analysis Project

ABC transporters are essential for cellular transport; contribute to maintain the homeostasis of the cells. Generally, ABC transporters are multi-subunit; contain essential cytoplasmic factors which are critical to ATP hydrolysis activity. In this paper, we would like to take a closer look to Mrub_1283, Mrub_1284 and Mrub_1285, three consecutive genes in Meiothermus ruber genome. We hypothesize that these genes are in the same operon and encode for ABC glycine/ betaine transporters. To confirm our hypothesis, we utilizes several bioinformatics tools to predict the potential function of Mrub_1283, Mrub_1284 and Mrub_1285 and to search for their orthologs in Escherichia coli genome ...


Mechanical Test Methods For Assessing Porcine Carotid And Uterine Artery Burst Pressure Following Ex Vivo Ultrasonic Ligature Seal And Transection, Carissa M. Krane, Margaret Pinnell, Courtney Gardner, Mercedes Thompson, James Coleman, Robert Wilkens 2017 University of Dayton

Mechanical Test Methods For Assessing Porcine Carotid And Uterine Artery Burst Pressure Following Ex Vivo Ultrasonic Ligature Seal And Transection, Carissa M. Krane, Margaret Pinnell, Courtney Gardner, Mercedes Thompson, James Coleman, Robert Wilkens

Robert J. Wilkens

A test method was developed to identify those variables important for assessing the performance of ultrasonic surgical devices in ex vivo ligature sealing of porcine carotid and uterine arteries. Ruggedness testing using a small sample size in pilot experiments was conducted using a newly developed test method in an effort to assess the usefulness of this methodology and to identify test variables that might warrant further testing. The development of this test method included the use of a custom-designed prototypic tension device for load-controlled ex vivo vessel stretching during saline perfusion and subsequent seal and transection of porcine arteries with ...


Genetically Modified Crops: Marvel Or Malady?, Peter Goldsbrough 2017 Purdue University

Genetically Modified Crops: Marvel Or Malady?, Peter Goldsbrough

Integrated Crop Management Conference

The first transgenic plants were produced in 1983 by three different groups of scientists in the United States and Europe. The successful transfer of genes from other species into petunia and tobacco plants was a major technological achievement that led to important advances in our understanding of how plants grow and develop. This technology also led to the development of genetically modified (GM) agronomic crop plants that were planted by farmers for the first time in 1996. This technology has generated more controversy than any other advance in agriculture in the last twenty years. Further scientific advances made during the ...


Optimizing Sperm Collection Procedures In Zebrafish, Madelyn B. Wasden, Rachel L. Roberts, April DeLaurier 2017 University of South Carolina Aiken

Optimizing Sperm Collection Procedures In Zebrafish, Madelyn B. Wasden, Rachel L. Roberts, April Delaurier

Journal of the South Carolina Academy of Science

Zebrafish are a highly-valued model organism used for developmental biology research. Zebrafish can be used for genetic manipulation and hence, many mutant and transgenic lines exist. It is impractical to maintain lines of adult zebrafish, due to resource constraints and the need to continuously produce new generations. Therefore, a practical way to preserve zebrafish lines is to freeze sperm and retrieve lines using in vitro fertilization of fresh eggs. Most existing in vitro protocols used by research labs have a wide variety of fertilization rates (ranging from 0% to >90%). Due to this variability, lines may be at risk of ...


Mitochondrial Reactive Oxygen Species In Lipotoxic Hearts Induces Post-Translational Modifications Of Akap121, Drp1 And Opa1 That Promote Mitochondrial Fission, Kensuke Tsushima, Heiko Bugger, Adam R. Wende, Jamie Soto, Gregory A. Jenson, Austin R. Tor, Rose McGlauflin, Helena C. Kenny, Yuan Zhang, Rhonda Souvenir, Xiao X. Hu, Crystal L. Sloan, Renata O. Pereira, Vitor A. Lira, Kenneth W. Spitzer, Terry L. Sharp, Kooresh I. Shoghi, Genevieve C. Sparagna, Eva A. Rog-Zielinska, Peter Kohl, Oleh Khalimonchuk, Jean E. Schaffer, E. Dale Abel 2017 University of Iowa

Mitochondrial Reactive Oxygen Species In Lipotoxic Hearts Induces Post-Translational Modifications Of Akap121, Drp1 And Opa1 That Promote Mitochondrial Fission, Kensuke Tsushima, Heiko Bugger, Adam R. Wende, Jamie Soto, Gregory A. Jenson, Austin R. Tor, Rose Mcglauflin, Helena C. Kenny, Yuan Zhang, Rhonda Souvenir, Xiao X. Hu, Crystal L. Sloan, Renata O. Pereira, Vitor A. Lira, Kenneth W. Spitzer, Terry L. Sharp, Kooresh I. Shoghi, Genevieve C. Sparagna, Eva A. Rog-Zielinska, Peter Kohl, Oleh Khalimonchuk, Jean E. Schaffer, E. Dale Abel

Biochemistry -- Faculty Publications

Rationale: Cardiac lipotoxicity, characterized by increased uptake, oxidation and accumulation of lipid intermediates, contributes to cardiac dysfunction in obesity and diabetes. However, mechanisms linking lipid overload and mitochondrial dysfunction are incompletely understood.

Objective: To elucidate the mechanisms for mitochondrial adaptations to lipid overload in postnatal hearts in vivo.

Methods and Results: Using a transgenic mouse model of cardiac lipotoxicity overexpressing long-chain acyl-CoA synthetase 1 in cardiomyocytes, we show that modestly increased myocardial fatty acid uptake leads to mitochondrial structural remodeling with significant reduction in minimum diameter. This is associated with increased palmitoyl-carnitine oxidation and increased reactive oxygen species (ROS) generation ...


Newest Kid On The Block: Characterization Of The Novel Multidrugresistant Pathogen, Candida Auris, Lindsey Masone, Susanna Mirabelli 2017 Long Island University

Newest Kid On The Block: Characterization Of The Novel Multidrugresistant Pathogen, Candida Auris, Lindsey Masone, Susanna Mirabelli

Post & Beyond

Candida auris is a novel and emerging fungal pathogen capable of causinginvasive, and often fatal, bloodstream and wound infections in immunocompromised patients. Several outbreaks have been reported in hospitals across the world, including the United States, and predominantly in New York. C.auris exhibits extensive multidrug resistance that has never been seen in any Candida species, and is often misdiagnosed for other Candida species, thus limiting treatment options for patients. Our laboratory obtained 10 C. auris isolates in order to perform in vitro antifungal susceptibility testing and determine the Minimum Inhibitory Concentration (MIC) under Clinical and Laboratory Standards Institute guidelines ...


Evaluation Of A Microalgal Bioassay Based On Community Level Physiological Profiling (Clpp), Jun-Woo Kim 2017 The University of Western Ontario

Evaluation Of A Microalgal Bioassay Based On Community Level Physiological Profiling (Clpp), Jun-Woo Kim

Electronic Thesis and Dissertation Repository

This thesis examined the use of commercially available Biolog 96-well plates containing different carbon sources for a microalgal bioassay. Quaternary ammonium compounds (QACs) were used as potentially toxic model compounds to demonstrate the applicability of the assay. Toxicity of dodecyl trimethyl ammonium chloride (DTAC) and didecyl dimethyl ammonium bromide (DDAB) on the growth of Scenedesmus obliquus varied significantly under autotrophic, heterotrophic and mixotrophic growth conditions. The concentration for 50% growth inhibition effect (EC50) of DTAC followed the order: autotrophic (0.48 ± 0.03 mg of DTAC/L) > heterotrophic (1.46 ± 0.04 mg of DTAC/L) > mixotrophic (2.11 ...


Purification From Human Milk Of Matriptase Complexes With Secreted Serpins: Mechanism For Inhibition Of Matriptase Other Than Hai-1, I-Chu Tseng, Feng-Pai Chou, Sheng-Feng Su, Michael Oberst, Nandakumar Madayiputhiya, Ming-Shyue Lee, Jehng-Kang Wang, David E. Sloane, Michael Johnson, Chen-Yong Lin 2017 University of Maryland at Baltimore

Purification From Human Milk Of Matriptase Complexes With Secreted Serpins: Mechanism For Inhibition Of Matriptase Other Than Hai-1, I-Chu Tseng, Feng-Pai Chou, Sheng-Feng Su, Michael Oberst, Nandakumar Madayiputhiya, Ming-Shyue Lee, Jehng-Kang Wang, David E. Sloane, Michael Johnson, Chen-Yong Lin

Michael C. Johnson

Matriptase, a type 2 transmembrane serine protease, is predominately expressed by epithelial and carcinoma cells in which hepatocyte growth factor activator inhibitor 1 (HAI-1), a membrane-bound, Kunitz-type serine protease inhibitor, is also expressed. HAI-1 plays dual roles in the regulation of matriptase, as a conventional protease inhibitor and as a factor required for zymogen activation of matriptase. As a consequence, activation of matriptase is immediately followed by HAI-1-mediated inhibition, with the activated matriptase being sequestered into HAI-1 complexes. Matriptase is also expressed by peripheral blood leukocytes, such as monocytes and macrophages; however, in contrast to epithelial cells, monocytes and macrophages ...


Ydj1 Governs Fungal Morphogenesis And Stress Response, And Facilitates Mitochondrial Protein Import Via Mas1 And Mas2, Jinglin L. Xie, Iryna Bohovych, Erin O.Y. Wong, Jean-Philippe Lambert, Anne-Claude Gingras, Oleh Khalimonchuk, Leah E. Cowen, Michelle D. Leach 2017 University of Aberdeen

Ydj1 Governs Fungal Morphogenesis And Stress Response, And Facilitates Mitochondrial Protein Import Via Mas1 And Mas2, Jinglin L. Xie, Iryna Bohovych, Erin O.Y. Wong, Jean-Philippe Lambert, Anne-Claude Gingras, Oleh Khalimonchuk, Leah E. Cowen, Michelle D. Leach

Biochemistry -- Faculty Publications

Mitochondria underpin metabolism, bioenergetics, signalling, development and cell death in eukaryotes. Most of the ~1,000 yeast mitochondrial proteins are encoded in the nucleus and synthesised as precursors in the cytosol, with mitochondrial import facilitated by molecular chaperones. Here, we focus on the Hsp40 chaperone Ydj1 in the fungal pathogen Candida albicans, finding that it is localised to both the cytosol and outer mitochondrial membrane, and is required for cellular stress responses and for filamentation, a key virulence trait. Mapping the Ydj1 protein interaction network highlighted connections with co-chaperones and regulators of filamentation. Furthermore, the mitochondrial processing peptidases Mas1 and ...


Plant Variety In The Republic Of Korea: Seed Industry Law, Yoonjin Buyn 2017 University of Oklahoma College of Law

Plant Variety In The Republic Of Korea: Seed Industry Law, Yoonjin Buyn

Oklahoma Journal of Law and Technology

No abstract provided.


The European Patent Office And The European Patent: An Open Avenue For Biotechnologists And "Living Inventions", Katrina McClatchey 2017 University of Oklahoma College of Law

The European Patent Office And The European Patent: An Open Avenue For Biotechnologists And "Living Inventions", Katrina Mcclatchey

Oklahoma Journal of Law and Technology

No abstract provided.


Agricultural Biogechnology: United States Statutory Law, Jo Lynn Jeter 2017 University of Oklahoma College of Law

Agricultural Biogechnology: United States Statutory Law, Jo Lynn Jeter

Oklahoma Journal of Law and Technology

No abstract provided.


The International Treaty On Plant Genetic Resources For Food And Agriculture: Friend Of The International Farmer, Steven M. Ruby 2017 University of Oklahoma College of Law

The International Treaty On Plant Genetic Resources For Food And Agriculture: Friend Of The International Farmer, Steven M. Ruby

Oklahoma Journal of Law and Technology

No abstract provided.


The Effect Of The "Onco-Mouse" Decisions On The Exception To Patentability For "Animal Varieties" Under The European Patent Convention, Katrina McClatchey 2017 University of Oklahoma College of Law

The Effect Of The "Onco-Mouse" Decisions On The Exception To Patentability For "Animal Varieties" Under The European Patent Convention, Katrina Mcclatchey

Oklahoma Journal of Law and Technology

No abstract provided.


The Impact Of Novartis On The European Patent Convention's Exception To Patentability For "Plant Varieties", Katrina McClatchey 2017 University of Oklahoma College of Law

The Impact Of Novartis On The European Patent Convention's Exception To Patentability For "Plant Varieties", Katrina Mcclatchey

Oklahoma Journal of Law and Technology

No abstract provided.


Agricultural Biotechnology: United States Case Law, Jo Lynn Jeter 2017 University of Oklahoma College of Law

Agricultural Biotechnology: United States Case Law, Jo Lynn Jeter

Oklahoma Journal of Law and Technology

No abstract provided.


Emerging Drivers And Business Models For Equipment Reuse And Remanufacturing In The Us Lessons From The Biotech Industry-2017.Pdf, Vesela R. Veleva, Gavin Bodkin 2017 University of Massachusetts Boston

Emerging Drivers And Business Models For Equipment Reuse And Remanufacturing In The Us Lessons From The Biotech Industry-2017.Pdf, Vesela R. Veleva, Gavin Bodkin

Vesela Veleva

While regulations have advanced product take-back in some markets, challenges to increasing product reuse and remanufacturing remain. Most research to date has focused on original equipment manufacturers (OEMs) taking back and remanufacturing their products, which is often problematic.  The present study demonstrates that there are emerging opportunities for small companies with innovative business models to enter the market and advance product end-of-life (EoL) management. The paper examines the biotechnology industry – a growing sector with high spending on lab equipment and relatively short lifespans of R&D instruments. Building on previous research and analysis of industry practices and emerging drivers for ...


Mixed Municipal Solid Waste Hygienisation For Refuse-Derived Fuel Production By Ozonation In The Novel Configuration Using Fluidized Bed And Horizontal Reactor, Witold Zukowski 2017 Cracow University of Technology

Mixed Municipal Solid Waste Hygienisation For Refuse-Derived Fuel Production By Ozonation In The Novel Configuration Using Fluidized Bed And Horizontal Reactor, Witold Zukowski

Witold Zukowski

Purpose
This study aimed to determine whether mixed municipal solid waste used for alternative fuel production can be effectively treated with ozone hygienisation in a gaseous phase.
Methods
It was determined that the use of a fluidised bed reactor and a horizontal reactor with respect to processing the light fraction and the heavy fraction of waste effectively contributes to the elimination or reduction of the number of selected groups of microorganisms inhabiting mixed municipal solid waste. With the use of microbiological analysis, the presence of vegetative bacteria, spores, fungi, actinomycetes and pathogenic bacteria, i.g. Staphylococcus spp., Escherichia coli, Salmonella ...


Identification Of Potential Tissue-Specific Cancer Biomarkers And Development Of Cancer Versus Normal Genomic Classifiers, Akram Mohammed, Greyson Biegert, Jiri Adamec, Tomáš Helikar 2017 University of Nebraska-Lincoln

Identification Of Potential Tissue-Specific Cancer Biomarkers And Development Of Cancer Versus Normal Genomic Classifiers, Akram Mohammed, Greyson Biegert, Jiri Adamec, Tomáš Helikar

Biochemistry -- Faculty Publications

Machine learning techniques for cancer prediction and biomarker discovery can hasten cancer detection and significantly improve prognosis. Recent “OMICS” studies which include a variety of cancer and normal tissue samples along with machine learning approaches have the potential to further accelerate such discovery. To demonstrate this potential, 2,175 gene expression samples from nine tissue types were obtained to identify gene sets whose expression is characteristic of each cancer class. Using random forests classification and ten-fold cross-validation, we developed nine single-tissue classifiers, two multi-tissue cancer-versus-normal classifiers, and one multi-tissue normal classifier. Given a sample of a specified tissue type, the ...


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