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417 full-text articles. Page 1 of 14.

Evaluation Of Novel Multi-Dimensional Tissue Culturing Methods Using Autonomously Bioluminescent Human Cell Lines, James Dean Webb 2014 University of Tennessee, Knoxville

Evaluation Of Novel Multi-Dimensional Tissue Culturing Methods Using Autonomously Bioluminescent Human Cell Lines, James Dean Webb

University of Tennessee Honors Thesis Projects

No abstract provided.


General Mills Turns To 'Raja Of The Country Of World Peace' To Certify Its Costlier, Non-Gmo Cheerios, Drew L. Kershen 2014 University of Oklahoma College of Law

General Mills Turns To 'Raja Of The Country Of World Peace' To Certify Its Costlier, Non-Gmo Cheerios, Drew L. Kershen

Drew L. Kershen

No abstract provided.


Tattletales And T-Bow Update 20140313thur, George McNamara 2014 The University of Texas

Tattletales And T-Bow Update 20140313thur, George Mcnamara

George McNamara

George McNamara 20140313Thur

http://works.bepress.com/gmcnamara/42

Tattletales and T-Bow Update

Please see also http://works.bepress.com/gmcnamara/16

Tattletales: multiplex fluorescent protein biosensors by spatial localization with TALE-FPs, Cas9-FPs, ZF-FPs, LacI-FPs, TetR-FPs, etc.

T-Bow: Rainbow T-cells and Tumor cells (and ES cells, iPS cells, other cells and organisms). You can think of this as "Brainbow meets TALENs/Cas9/ZFNs/other DNA sequence specific binding proteins".

If not familiar with Brainbow, see

http://en.wikipedia.org/wiki/Brainbow

If not familiar with TALENs, Cas9, etc, see

http://www.addgene.org/genome_engineering/

Big idea: localizing fluorescent proteins - and ...


Using Progesterone As An Indicator Of Ovarian Response To Stimulation In Cattle, Kayla Mariah Kauffman 2014 California Polytechnic State University

Using Progesterone As An Indicator Of Ovarian Response To Stimulation In Cattle, Kayla Mariah Kauffman

Animal Science

No abstract provided.


Zyflamend, A Polyherbal Mixture, Down Regulates Class I And Class Ii Histone Deacetylases And Increases P21 Levels In Castrate-Resistant Prostate Cancer Cells, E-Chu Huang, Yi Zhao, Guoxun Chen, Seung Joon Baek, Michael F. McEntee, Steven Minkin, John P. Biggerstaff, Jay Whelan 2014 University of Tennessee, Knoxville

Zyflamend, A Polyherbal Mixture, Down Regulates Class I And Class Ii Histone Deacetylases And Increases P21 Levels In Castrate-Resistant Prostate Cancer Cells, E-Chu Huang, Yi Zhao, Guoxun Chen, Seung Joon Baek, Michael F. Mcentee, Steven Minkin, John P. Biggerstaff, Jay Whelan

Nutrition Publications and Other Works

Background

Zyflamend, a mixture containing extracts of ten herbs, has shown promise in a variety of preclinical cancer models, including prostate cancer. The current experiments were designed to investigate the effects of Zyflamend on the expression of class I and II histone deacetylases, a family of enzymes known to be over expressed in a variety of cancers.

Methods

CWR22Rv1 cells, a castrate-resistant prostate cancer cell line, were treated with Zyflamend and the expression of class I and II histone deacetylases, along with their downstream target the tumor suppressor gene p21, was investigated. Involvement of p21 was confirmed with siRNA knockdown ...


Arizona Clinical Research Workforce Survey, Cris E. Wells, Julie A. Robbins, Gaye Luna 2014 Southern Illinois University Carbondale

Arizona Clinical Research Workforce Survey, Cris E. Wells, Julie A. Robbins, Gaye Luna

Online Journal for Workforce Education and Development

Objective: We characterize the current clinical research workforce in Arizona with the goal of identifying possible educational training gaps for the future workforce. Background: The science community, government entities, educational institutions, and community programs continue to advocate for additional funding and resources to educate the bioscience workforce, of which clinical research is a part. However, there is little evidence that additional training and/or education is needed within the clinical research profession. Method: We utilized an electronic survey tool that was designed to solicit workforce data from clinical research professionals actively working or seeking employment in the field of clinical ...


Time Series Data For 3b Image Processing, George McNamara 2014 The University of Texas

Time Series Data For 3b Image Processing, George Mcnamara

George McNamara

Time series data for 3B image processing

Four time series data sets of Stellaris single molecules RNA FISH (fluorescence in situ hybridization). All four datasets are 301 imaqe planes. FISH probes are to TOP1 mRNA (Topoisomerase I)in Saos osterosarcoma cells.

One dataset is 10 millisecond exposures (very dim), acquired in Streaming acquisition mode (no hardware overhead).

The other three datasets are a three consecutive planes of the same XY field of view.

I have included image processing results for:

PiMP - a fast method developed by Sebastian Munck et al 2012, http://www.ncbi.nlm.nih.gov/pubmed/?term=22357945 ...


The Effects Of Salinity, Ph, Temperature, And Dissolved Oxygen On Sensitivity Of Pcr Identification Of T4 Bacteriophage, Joesph F. Cannon, Nicholas A. Thurn, Paul E. Richardson 2013 University of South Carolina

The Effects Of Salinity, Ph, Temperature, And Dissolved Oxygen On Sensitivity Of Pcr Identification Of T4 Bacteriophage, Joesph F. Cannon, Nicholas A. Thurn, Paul E. Richardson

Journal of the South Carolina Academy of Science

Bacteriophages are used as indicators of pathogenic bacteria in drinking, and wastewaters. They also show potential in limiting aquatic bacterial populations through their lytic properties. The effect of different water characteristics (salinity, pH, dissolved oxygen, and temperature) on the sensitivity of the PCR identification of virus particles were analyzed to determine at what levels bacteriophage can be detected in environmental samples. Results from this preliminary study indicate that a PCR bacteriophage detection technique has potential as a relatively efficient and economical indicator of coliform contamination in multiple aquatic environments. While further evaluation is needed, the protocol appears to function in ...


Role Of Carotenoid Cleavage Dioxygenases In Volatile Emissions And Insect Resistance In Arabidopsis, Shailu Lakshminarayan 2013 Western University

Role Of Carotenoid Cleavage Dioxygenases In Volatile Emissions And Insect Resistance In Arabidopsis, Shailu Lakshminarayan

University of Western Ontario - Electronic Thesis and Dissertation Repository

In Arabidopsis thaliana, carotenoid degradation by enzymatic oxidative cleavage is catalyzed by nine CCD and NCED enzymes; this cleavage produces an array of terpenoid products that are collectively known as apocarotenoids, which include volatile and non-volatile compounds. Previous studies on CCD1 revealed that its overexpression in A. thaliana enhances β-ionone emission and reduces damage caused by flea beetles. In consideration of these findings, I generated CCD4 and CCD8 Arabidopsis overexpression lines. Further, I characterized these overexpression lines on the basis of their carotenoid content and their volatile profiles. Surprisingly, accumulation of key carotenoids in the leaves was observed in both ...


Ogden College Of Science & Engineering Newsletter, Odgen College of Science & Engineering, Western Kentucky University, Cheryl Stevens, Dean 2013 Western Kentucky University

Ogden College Of Science & Engineering Newsletter, Odgen College Of Science & Engineering, Western Kentucky University, Cheryl Stevens, Dean

Ogden College of Science & Engineering Publications

No abstract provided.


Near-Infrared Surface-Enhanced Fluorescence Using Silver Nanoparticles In Solution, Michael D. Furtaw 2013 University of Nebraska - Lincoln

Near-Infrared Surface-Enhanced Fluorescence Using Silver Nanoparticles In Solution, Michael D. Furtaw

Mechanical (and Materials) Engineering -- Dissertations, Theses, and Student Research

Fluorescence spectroscopy is a widely used detection technology in many research and clinical assays. Further improvement to assay sensitivity may enable earlier diagnosis of disease, novel biomarker discovery, and ultimately, improved outcomes of clinical care along with reduction in costs. Near-infrared, surface-enhanced fluorescence (NIR-SEF) is a promising approach to improve assay sensitivity via simultaneous increase in signal with a reduction in background. This dissertation describes research conducted with the overall goal to determine the extent to which fluorescence in solution may be enhanced by altering specific variables involved in the formation of plasmonactive nanostructures of dye-labeled protein and silver nanoparticles ...


Heterologous Production And Charaterization Of Cell Wall Degrading Enzymes Using Plants As A Bioreactor, Eridan Orlando Rodrigues Pereira 2013 Western University

Heterologous Production And Charaterization Of Cell Wall Degrading Enzymes Using Plants As A Bioreactor, Eridan Orlando Rodrigues Pereira

University of Western Ontario - Electronic Thesis and Dissertation Repository

Plants are wonderful living organisms. They are able to store solar energy into carbohydrates by fixing CO2 through photosynthesis which can be subsequently harvested and used for fuel production. However, one of the major limitations for transforming these carbohydrates into liquid fuels is the recalcitrance of the plant cell wall. Although microorganisms have evolved a series of cell wall degrading enzymes to harvest efficiently this energy and are considered the main source of these biocatalysts, harnessing these microorganisms for the production of enzymes is a costly process and a major factor limiting the commercialization of lignocellulosic biomass-to-ethanol processes. The ...


Detection Of Foodborne Pathogens By Micro-Filtration Using A Continuous Cell Concentrator Device, Klaire E. Jeffries, Eduardo Ximenes, Michael R. Ladisch 2013 Purdue University

Detection Of Foodborne Pathogens By Micro-Filtration Using A Continuous Cell Concentrator Device, Klaire E. Jeffries, Eduardo Ximenes, Michael R. Ladisch

The Summer Undergraduate Research Fellowship (SURF) Symposium

Protecting consumers from foodborne illness is an important health concern facing the food industry today. An important deficiency exposed by foodborne illness is the inability to track contaminated food back to the source in a timely manner. Although there are established methods that detect bacterial pathogen contamination, they are limited in distinguishing viable bacteria reliably and quickly. Currently, food pathogen testing requires lengthy culture steps, which many times are delayed even longer due to the lack of in-house testing labs. Typically, two to three days elapses between when the food is sampled and the test results are available. This study ...


Bicistronic Design For Precise And Reliable Gene Expression, Nidhi N. Menon, Jenna Rickus 2013 Purdue University

Bicistronic Design For Precise And Reliable Gene Expression, Nidhi N. Menon, Jenna Rickus

The Summer Undergraduate Research Fellowship (SURF) Symposium

Despite having progressed extensively in the field of synthetic biology in terms of DNA synthesis, analysis and transplanting, we still cannot reliably, quantitatively measure expression of new genetic constructs. We engineered a biobrick compatible expression cassette to control transcription and translation initiation which can be reused in new genetic contexts. Previous research has shown that the Bicistronic design have much lesser variations in expression with varying genes of interest as compared to the regular monocistronic design.(Mutalik, Endy, Guimaraes, Cambray, Lam, Juul, Tran & Paull, 2013) The Bicistronic design(BCD) consists of two Shine-Dalgarno sequences in its translation element which when ...


Artificial Yeast Polarization Controlled By Chemical Gradient, James K. Nolan, Bernard Tao 2013 Purdue University

Artificial Yeast Polarization Controlled By Chemical Gradient, James K. Nolan, Bernard Tao

The Summer Undergraduate Research Fellowship (SURF) Symposium

Engineering synthetic multicellular systems will lead to new synthetic biology technological platforms, inform developmental biology through recapitulation of natural systems and possibly unveil novel morphologies with practical applications not before reached throughout natural history (Maharbiz, 2012). Creating an exogenous molecular circuit that will polarize unicellular cells into “apical” and “basal” domains relative to a substrate plane would fulfill a missing component towards fully multicellular synthetic cellular communities (Maharbiz, 2012). To this end, a PIP3 polarization network previously designed by Chau and associates (Chau, Walter, Gerardin, Tang, Lim 2012) was coupled to the specific activation by niacin of a recombinant ...


Development Of A Metabolomic Method To Define The Phenylalanome In Arabidopsis Thaliana, Cole G. Wunderlich, Clint Chapple, Xu Li 2013 Purdue University

Development Of A Metabolomic Method To Define The Phenylalanome In Arabidopsis Thaliana, Cole G. Wunderlich, Clint Chapple, Xu Li

The Summer Undergraduate Research Fellowship (SURF) Symposium

In the study of metabolomics, one of the greatest challenges can be accurately identifying compounds detected in biological extracts, especially when standards are not readily available. Current metabolomic methods are also limited in that they provide little to no information about a compound’s metabolic origin. In this study, we sought to address these issues by developing a novel metabolomic method that employs stable isotope feeding, LC-MS, Xcms, and an analytical software algorithm to study the ‘phenylalanome’ of Arabidopsis thaliana. Using this approach we were able to develop a method that, based on current results, is capable of detecting over ...


Fabrication Of Multilayered Structure For Coherent Random Lasing, John Rauchenstein, Young L. Kim 2013 Purdue University

Fabrication Of Multilayered Structure For Coherent Random Lasing, John Rauchenstein, Young L. Kim

The Summer Undergraduate Research Fellowship (SURF) Symposium

High powered lasers have many applications, including medical treatment and surgery. However, these lasers are extremely expensive and are therefore not widely available. The aim of this study was to demonstrate a method to create such a laser with significantly decreased overall cost and increased efficiency. In order to do this, we explored a phenomenon called random lasing which is a light amplification process. To start with, a low-cost pumping laser is directed at normal incidence toward a multi-layered sample with two alternating layers. At first pearl, a naturally found material that has many organic nano-scale layers (similar to the ...


Why Is Charcoal So Effective For Plant Growth?, Makoto Ogawa 2013 University of Massachusetts - Amherst

Why Is Charcoal So Effective For Plant Growth?, Makoto Ogawa

USBI Biochar Conferences

No abstract provided.


Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, Rajesh Pati 2013 SelectedWorks

Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, Rajesh Pati

Rajesh Pati

A standard uniform protocol of regeneration is the prime and foremost prerequisite for not only improve the productivity, the production from the existing area but also development of transgenics in mango for various traits. Polyembryonic cultivars are good rootstock because of moderate canopy, tolerant to draught, salt, environmental stress (wind, high temperature, air pollution), fungal disease (anthracnose, powdery mildew, gummosis) and hoppers.


Genetic Transformation Of Bael (Aegle Marmelos Corr.), Rajesh Pati 2013 SelectedWorks

Genetic Transformation Of Bael (Aegle Marmelos Corr.), Rajesh Pati

Rajesh Pati

Aegle marmelos Corr. is better known for its medicinal values rather than its edible quality. Every part of the plant viz. leaves, fruits, roots, and the bark are used for treating various diseases related to heart, stomach, bacterial, fungal, viral, worms, fertility, fever and cosmetics. Marmelosin is one of the major compounds (coumarin), used to treat various diseases. Till date, there are no varieties developed for commercial orchards of Bael and hence, regarded as one among the underutilized fruits of India. Therefore, there is an urgent need for breeding new and cultivable varieties in Aegle marmelos. But the problem for ...


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