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Thomas, Norman (Fa 1129), Manuscripts & Folklife Archives 2018 Western Kentucky University

Thomas, Norman (Fa 1129), Manuscripts & Folklife Archives

FA Finding Aids

Finding aid only for Folklife Archives Project 1129. Paper titled “Traditional Recipes” in which Norman Thomas details how to prepare various dishes ranging from opossum and porcupine to maple syrup and molasses taffy. Paper is based on information collected by Thomas from close relatives and family friends.


Salmonella And Fecal Indicator Bacteria Survival In Soils Amended With Poultry Manure, Claire E. Hruby, Michelle L. Soupir, Thomas B. Moorman, Carl H. Pederson, Rameshwar S. Kanwar 2018 Iowa State University

Salmonella And Fecal Indicator Bacteria Survival In Soils Amended With Poultry Manure, Claire E. Hruby, Michelle L. Soupir, Thomas B. Moorman, Carl H. Pederson, Rameshwar S. Kanwar

Agricultural and Biosystems Engineering Publications

Minimizing the risks associated with manure-borne pathogenic microorganisms requires an understanding of microbial survival under realistic field conditions. The objective of this 3-year study was to assess the fate of Salmonella (SALM) and fecal indicator bacteria (FIB), E. coli (EC) and enterococci (ENT), in glacial till-derived soils, after application of poultry manure (PM) to cornfields under chisel-plowed (CP) or no-till (NT) management. From 2010 to 2012, soil samples were obtained each spring at 0–15- and 15–30-cm depths, to determine whether over-wintering of target bacteria had occurred. Sampling was followed by application of PM at low (PM1) and high ...


Design And Evaluation Of 16s Rrna-Targeted Peptide Nucleic Acid Probes For Whole-Cell Detection Of Members Of The Genus Listeria, Byron F. Brehm-Stecher, Jens J. Hyldig-Nielsen, Eric A. Johnson 2018 Iowa State University

Design And Evaluation Of 16s Rrna-Targeted Peptide Nucleic Acid Probes For Whole-Cell Detection Of Members Of The Genus Listeria, Byron F. Brehm-Stecher, Jens J. Hyldig-Nielsen, Eric A. Johnson

Byron F. Brehm-Stecher

Six fluorescein-labeled peptide nucleic acid oligomers targeting Listeria-specific sequences on the 16S ribosomal subunit were evaluated for their abilities to hybridize to whole cells by fluorescence in situ hybridization (FISH). Four of these probes yielded weak or no fluorescent signals after hybridization and were not investigated further. The remaining two FISH-compatible probes, LisUn-3 and LisUn-11, were evaluated for their reactivities against 22 Listeria strains and 17 other bacterial strains belonging to 10 closely related genera. Hybridization with BacUni-1, a domain-specific eubacterial probe, was used as a positive control for target accessibility in both Listeria spp. and nontarget cells. RNase ...


Flow Cytometry For Rapid Detection Of Salmonella Spp. In Seed Sprouts, Bledar Bisha, Byron F. Brehm-Stecher 2018 University of Wyoming

Flow Cytometry For Rapid Detection Of Salmonella Spp. In Seed Sprouts, Bledar Bisha, Byron F. Brehm-Stecher

Byron F. Brehm-Stecher

Seed sprouts (alfalfa, mung bean, radish, etc.) have been implicated in several recent national and international outbreaks of salmonellosis. Conditions used for sprouting are also conducive to the growth of Salmonella. As a result, this pathogen can quickly grow to very high cell densities during sprouting without any detectable organoleptic impact. Seed sprouts typically also support heavy growth (~108 CFU g−1) of a heterogeneous microbiota consisting of various bacterial, yeast, and mold species, often dominated by non-pathogenic members of the family Enterobacteriaceae. This heavy background may present challenges to the detection of Salmonella, especially if this pathogen is present ...


Sodium Polyphosphate And Polyethylenimine Enhance The Antimicrobial Activities Of Plant Essential Oils, Heidi A. Wright, Byron F. Brehm-Stecher 2018 Iowa State University

Sodium Polyphosphate And Polyethylenimine Enhance The Antimicrobial Activities Of Plant Essential Oils, Heidi A. Wright, Byron F. Brehm-Stecher

Byron F. Brehm-Stecher

Plant extracts have been used for millennia for treatment of disease, with much recent interest focusing on the antimicrobial activities of plant essential oils (EOs). Although EOs are active against common microbial pathogens, their effective use as topical, environmental, or food antimicrobials will require EO-based formulations with enhanced antimicrobial activities. In this study, two polyionic compounds, sodium polyphosphate (polyP, a polyanion) and polyethylenimine (PEI, a polycation), were evaluated for their abilities to enhance the antimicrobial activities of six EOs against the human pathogens Escherichia coli O157:H7, Salmonella enterica subsp. enterica ser. Minnesota, Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus aureus, and ...


Design And Evaluation Of Peptide Nucleic Acid Probes For Specific Identification Of Candida Albicans., H. J. Kim, Byron F. Brehm-Stecher 2018 Iowa State University

Design And Evaluation Of Peptide Nucleic Acid Probes For Specific Identification Of Candida Albicans., H. J. Kim, Byron F. Brehm-Stecher

Byron F. Brehm-Stecher

Candida albicans is an important cause of systemic fungal infections, and rapid diagnostics for identifying and differentiating C. albicans from other Candida species are critical for the timely application of appropriate antimicrobial therapy, improved patient outcomes, and pharmaceutical cost savings. In this work, two 28S rRNA-directed peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) probes, P-Ca726 (targeting a novel region of the ribosome) and P-CalB2208 (targeting a previously reported region), were evaluated. Hybridization conditions were optimized by using both fluorescence microscopy (FM) and flow cytometry (FCM), and probes were screened for specificity and discriminative ability against a panel of C. albicans ...


Wide-Spectrum Biomimetic Antimicrobial Systems, Heidi A. Wright, Byron F. Brehm-Stecher 2018 Iowa State University

Wide-Spectrum Biomimetic Antimicrobial Systems, Heidi A. Wright, Byron F. Brehm-Stecher

Byron F. Brehm-Stecher

Antimicrobial peptides (AMPs) are effective components of the host immune response and are widely distributed throughout nature. Recently, nontoxic antimicrobial polymers that mimic the structures of naturally occurring AMPs have been designed and are under development commercially as novel therapeutics. These compounds have several potential advantages over natural AMPs, including greater stability and reduced immunogenicity compared to natural peptides, relatively simple and scalable syntheses and the ability to tailor or “fine tune” their activities through combinatorial approaches. In previous work, we demonstrated the utility of certain generally regarded as safe (GRAS) flavorant and aroma compounds as enhancers of uptake and ...


Murphy, Pat (Fa 1125), Manuscripts & Folklife Archives 2018 Western Kentucky University

Murphy, Pat (Fa 1125), Manuscripts & Folklife Archives

FA Finding Aids

Finding aid only for Folklife Archives Project 1125. Student paper titled “Wine-Making (Zymurgy)” in which Pat Murphy details the process of producing homemade wine. Murphy’s informants, which include close family members and friends, describe the growing and harvesting of grapes and blackberries, the fermentation period, ingredients, bottling, and distribution. The paper also includes interview transcripts.


Arizona Registered Dietitians Show Gaps In Knowledge Of Bean Health Benefits, Donna M. Winham, Andrea M. Hutchins, Sharon V. Thompson, Mariah K. Dougherty 2018 Iowa State University

Arizona Registered Dietitians Show Gaps In Knowledge Of Bean Health Benefits, Donna M. Winham, Andrea M. Hutchins, Sharon V. Thompson, Mariah K. Dougherty

Donna Winham

Registered Dietitians (RDs) promote nutrition practices and policies and can influence food consumption patterns to include nutrient dense foods such as beans. Although many evidence-based health benefits of bean consumption (e.g., cholesterol reduction, glycemic control) have been demonstrated, there is limited research on the knowledge, attitudes, and perceptions of RDs regarding the inclusion of beans in a healthy diet. To fill this existing research gap, this cross-sectional survey explored the perceptions, knowledge, and attitudes of 296 RDs in Arizona, USA, toward beans. The RDs largely held positive attitudes toward the healthfulness of beans and were aware of many health ...


Glycemic Response To Black Beans And Chickpeas As Part Of A Rice Meal: A Randomized Cross-Over Trial, Donna M. Winham, Andrea M. Hutchins, Sharon V. Thompson 2018 Iowa State University

Glycemic Response To Black Beans And Chickpeas As Part Of A Rice Meal: A Randomized Cross-Over Trial, Donna M. Winham, Andrea M. Hutchins, Sharon V. Thompson

Donna Winham

Legumes, such as black beans (Phaseolus vulgaris L.) and chickpeas (Cicer arietinum L.), have a low glycemic index, and may reduce the glycemic load of meals in which they are included. Although the low glycemic response of beans consumed alone has been documented, few studies have examined the glycemic response to traditional food combinations such as black beans and rice or chickpeas and rice. This randomized cross-over study examined the glycemic and insulinemic impact of 50 grams of available carbohydrate from three test meals: plain white rice (control), black beans with rice, and chickpeas with rice among healthy adult women ...


Knowledge Gaps Of The Health Benefits Of Beans Among Low-Income Women, Shelly M. Palmer, Donna Winham, Christine A. Hradek MPH 2018 Iowa State University

Knowledge Gaps Of The Health Benefits Of Beans Among Low-Income Women, Shelly M. Palmer, Donna Winham, Christine A. Hradek Mph

Donna Winham

Objectives: We determined knowledge of the health benefits of consuming beans, and assessed if awareness varied by acculturation status among Hispanic and non-Hispanic low-income women. Methods: We used a self-administered survey with Iowa women aged 18-65 years who were eligible to receive income-based services through 2 healthcare clinics, a WIC clinic, and Extension Outreach. Chi-square and ANOVA were used to compare bean health benefit knowledge, demographics, health-risk factors, nutrition information seeking, and self-efficacy by acculturation categories. Results: Of the 158 women who completed the survey, 58% were Hispanic, with a mean age of 36 years. In terms of acculturation, 24 ...


Interaction Of Cocoa Powder With Intestinal Microbiota, Zuyin Li 2018 Louisiana State University

Interaction Of Cocoa Powder With Intestinal Microbiota, Zuyin Li

LSU Doctoral Dissertations

Cocoa powder contains polyphenolic compounds, which can provide potential health benefits to humans. The human gastroinestinal tract hosts diverse microorganisms which are called the human gut microbiota. These microbes can utilize carbohydrates through fermentation resulting in the production of short chain fatty acids (SCFAs), which cause a reduction of pH in gastroinestinal tract. The cocoa processing can change the composition of the carbohydrates, which can affect the fermentation patterns.

In this study we established an in vitro model system which simulated gastric digestion and colon fermentation. Five different processed cocoa samples (alkalized cocoa samples: D-11-B and D-11-S; non-alkalized cocoa samples ...


Enhanced Destruction Of Salmonella Enterica In Carrot And Berry Juices By A Combination Of Cinnamaldehyde And High Pressure Processing, aura Darabe, Aubrey F. Mendonca, David Manu, James S. Dickson, Joseph G. Sebranek, Angela M. Shaw, Alan A. DiSpirito 2018 Iowa State University

Enhanced Destruction Of Salmonella Enterica In Carrot And Berry Juices By A Combination Of Cinnamaldehyde And High Pressure Processing, Aura Darabe, Aubrey F. Mendonca, David Manu, James S. Dickson, Joseph G. Sebranek, Angela M. Shaw, Alan A. Dispirito

Angela M. Shaw

Statement of the Problem: In recent years several disease outbreaks were linked to unpasteurized juices contaminated with human enteric pathogens such as Salmonella enterica and Escherichia coli O157:H7 (CDC 2011; EFSA 2015). While heat pasteurization and canning can inactivate vegetative pathogens, such processes can destroy heat labile nutrients and negatively alter sensory characteristics of juices. Also growing consumer demands for foods which are nutritious, fresh-like, and devoid of synthetic preservatives, have forced juice manufacturers to explore non-thermal processes and natural antimicrobials for pathogen control in juices. The present study investigated the effect of low concentrations of cinnamaldehyde combined with ...


The Effects Of Produce Washes On The Quality And Shelf Life Of "Cantaloupe" (Cucumis Melo Var. Cantalupensis) And "Watermelon" (Cirtullus Lantus Var. Lanatus), Amanda Svoboda, Angela M. Shaw, Lester A. Wilson, Aubrey F. Mendonca, Ajay Nair, Aura Daraba 2018 Iowa State University

The Effects Of Produce Washes On The Quality And Shelf Life Of "Cantaloupe" (Cucumis Melo Var. Cantalupensis) And "Watermelon" (Cirtullus Lantus Var. Lanatus), Amanda Svoboda, Angela M. Shaw, Lester A. Wilson, Aubrey F. Mendonca, Ajay Nair, Aura Daraba

Angela M. Shaw

Our research objective was to evaluate the ability of produce washes to maintain the texture and color quality attributes of watermelon and cantaloupe while reducing the levels of natural fungi population on the melon surface. Melons were submerged for 2 min into either water control or water containing quaternary ammonium chloride (300 ppm) or an 18% hydrogen peroxide and 12% peroxyacetic acid combination (100 ppm), or an acetic acid, peroxyacetic acid and hydrogen peroxide combination (0.78%). Microbial analysis and instrumental measurements were utilized to assess the melons on day 0, 7, 14 and 21 in three separate trials. The ...


"Clickers" And Haccp: Educating A Diverse Food Industry Audience With Technology, Angela M. Shaw, Aubrey F. Mendonca, Aura Daraba 2018 Iowa State University

"Clickers" And Haccp: Educating A Diverse Food Industry Audience With Technology, Angela M. Shaw, Aubrey F. Mendonca, Aura Daraba

Angela M. Shaw

Hazard Analysis Critical Control Points (HACCP) is a systematic approach to food safety education for the food industry. To receive a HACCP certificate, participants must receive an 80% or higher on the final examination. Language barriers, educational levels, and age have been noted as primary reasoning's for not passing the final examination. Clicker technology has been shown to improve knowledge transfer to students in various classroom settings. Incorporation of mock final examination questions using Clickers into a traditional HACCP course has been shown in a small pilot study to increase the pass rate.


Knowledge Gained From Good Agricultural Practices Courses For Iowa Growers, Angela M. Shaw, Catherine H. Strohbehn, Linda L. Naeve, Paul A. Domoto, Lester A. Wilson 2018 Iowa State University

Knowledge Gained From Good Agricultural Practices Courses For Iowa Growers, Angela M. Shaw, Catherine H. Strohbehn, Linda L. Naeve, Paul A. Domoto, Lester A. Wilson

Angela M. Shaw

Good Agricultural Practices (GAP) educational courses provide produce growers with the fundamental information for producing and processing safe produce. To determine the effectiveness of the current 7- hour GAP course provided in Iowa, growers were surveyed before and 7-14 days after the course to determine changes in knowledge and opinions. Results show that growers positively changed their knowledge and opinions on key food safety principles and regulations, which provides evidence that Extension programming is an effective method to educate small growers. Food safety educators should focus their training on practical methods for documentation, sanitation, and facilities.


Arizona Registered Dietitians Show Gaps In Knowledge Of Bean Health Benefits, Donna M. Winham, Andrea M. Hutchins, Sharon V. Thompson, Mariah K. Dougherty 2018 Iowa State University

Arizona Registered Dietitians Show Gaps In Knowledge Of Bean Health Benefits, Donna M. Winham, Andrea M. Hutchins, Sharon V. Thompson, Mariah K. Dougherty

Food Science and Human Nutrition Publications

Registered Dietitians (RDs) promote nutrition practices and policies and can influence food consumption patterns to include nutrient dense foods such as beans. Although many evidence-based health benefits of bean consumption (e.g., cholesterol reduction, glycemic control) have been demonstrated, there is limited research on the knowledge, attitudes, and perceptions of RDs regarding the inclusion of beans in a healthy diet. To fill this existing research gap, this cross-sectional survey explored the perceptions, knowledge, and attitudes of 296 RDs in Arizona, USA, toward beans. The RDs largely held positive attitudes toward the healthfulness of beans and were aware of many health ...


Influence Of Dietary Ractopamine And Supranutritional Supplementation Of Vitamin E On Proteome Profile Of Postmortem Beef Longissimus Lumborum Muscle, Hyun Mok Kim 2018 University of Kentucky

Influence Of Dietary Ractopamine And Supranutritional Supplementation Of Vitamin E On Proteome Profile Of Postmortem Beef Longissimus Lumborum Muscle, Hyun Mok Kim

Theses and Dissertations--Animal and Food Sciences

The effects of dietary ingredients on the proteome profile of postmortem beef longissimus lumborum (LL) muscle were evaluated. In the first experiment, the influence of dietary ractopamine on the whole-muscle proteome of beef LL was examined. Five proteins were differentially abundant between ractopamine-fed (RAC) and non-ractopamine fed (CON) groups. The differentially abundant proteins were over-abundant in RAC and were related to muscle structure development (F-actin-capping protein subunit beta-2 and PDZ and LIM domain protein-3), chaperone (heat shock protein beta-1), oxygen transportation (myoglobin), and glycolysis (L-lactate dehydrogenase A chain). These findings indicated that ractopamine influences the abundance of proteins associated with ...


Knowledge Gaps Of The Health Benefits Of Beans Among Low-Income Women, Shelly M. Palmer, Donna Winham, Christine A. Hradek MPH 2018 Iowa State University

Knowledge Gaps Of The Health Benefits Of Beans Among Low-Income Women, Shelly M. Palmer, Donna Winham, Christine A. Hradek Mph

Food Science and Human Nutrition Publications

Objectives: We determined knowledge of the health benefits of consuming beans, and assessed if awareness varied by acculturation status among Hispanic and non-Hispanic low-income women. Methods: We used a self-administered survey with Iowa women aged 18-65 years who were eligible to receive income-based services through 2 healthcare clinics, a WIC clinic, and Extension Outreach. Chi-square and ANOVA were used to compare bean health benefit knowledge, demographics, health-risk factors, nutrition information seeking, and self-efficacy by acculturation categories. Results: Of the 158 women who completed the survey, 58% were Hispanic, with a mean age of 36 years. In terms of acculturation, 24 ...


Nutritional, Nutraceutical And Functional Properties Of Soybeans, Suzanne Hendrich 2017 Iowa State University

Nutritional, Nutraceutical And Functional Properties Of Soybeans, Suzanne Hendrich

Food Science and Human Nutrition Publications

Soybeans, foods derived from soybeans (e.g., tofu, soymilk, soy infant formula, tempeh) and food and dietary supplement ingredients derived from soybeans (e.g., soybean oil, soybean proteins, isoflavones) have been under intensive research for their health effects especially over the past 25 years. 2 This intensive research derives from the recognition of soybeans as having desirable nutritional properties, containing about twice the protein of other legumes/serving, with good protein quality, such that some soybean protein ingredients have protein digestibility corrected amino acid scores commensurate with proteins thought to be optimal to meet human protein needs (Messina 1999). Soybean ...


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