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Growth Characteristics Of Lactobacillus Wasatchensis And Its Detection And Enumeration Using Quantitative Polymerase Chain Reaction, Isaac Brockbank Bowen 2018 Utah State University

Growth Characteristics Of Lactobacillus Wasatchensis And Its Detection And Enumeration Using Quantitative Polymerase Chain Reaction, Isaac Brockbank Bowen

All Graduate Theses and Dissertations

There are numerous challenges encountered during the manufacturing and storage of cheese by both the large-scale and artesian producers. One such challenge has been the formation of late gassy defect, which occurs when gas is produced by certain lactic acid bacteria found in the cheese block during storage and aging over a three month time period. Negative consequences of late gas production are slits and cracks in the cheese block and puffy cheese packaging, which cause significant financial losses for manufacturers along with poor consumer acceptance.

Lactobacillus wasatchensis is one such lactic acid bacterium shown to produce gas during cheese ...


Reporter Bacteriophage T7nlc Utilizes A Novel Nanoluc::Cbm Fusion For The Ultrasensitive Detection Of Escherichia Coli In Water., T.C. Hinkley, S. Garing, S. Singh, A-L. M. Le Ny, K.P. Nichols, J.E. Peters, Joey N. Talbert, S.R. Nugen 2018 Cornell University

Reporter Bacteriophage T7nlc Utilizes A Novel Nanoluc::Cbm Fusion For The Ultrasensitive Detection Of Escherichia Coli In Water., T.C. Hinkley, S. Garing, S. Singh, A-L. M. Le Ny, K.P. Nichols, J.E. Peters, Joey N. Talbert, S.R. Nugen

Food Science and Human Nutrition Publications

Rapid detection of bacteria responsible for foodborne diseases is a growing necessity for public health. Reporter bacteriophages (phages) are robust biorecognition elements uniquely suited for the rapid and sensitive detection of bacterial species. The advantages of phages include their host specificity, ability to distinguish viable and non-viable cells, low cost, and ease of genetic engineering. Upon infection with reporter phages, target bacteria express reporter enzymes encoded within the phage genome. In this study, the T7 coliphage was genetically engineered to express the newly developed luceriferase, NanoLuc (NLuc), as an indicator of bacterial contamination. While several genetic approaches were employed to ...


Implementation Of Iso/Iec Practices In Small And Academic Laboratories, Eric Layne Oliver 2018 University of Nebraska-Lincoln

Implementation Of Iso/Iec Practices In Small And Academic Laboratories, Eric Layne Oliver

Dissertations, Theses, & Student Research in Food Science and Technology

Food Manufacturers are facing challenging times due to regulations such as the Food Safety Modernization Act (FSMA) requiring them to provide evidence they are producing safe foods. Food testing laboratories aid in the mitigation of food safety issues providing evidence that a manufacturers food safety system is acceptable. To perform these activities laboratories are required to adhere to certain standards such as ISO/IEC 17025 General Requirements for the Competence of Testing and Calibration Laboratories. However, implementation of ISO/IEC 17025 practices is challenging, especially for small and academic laboratories, due to lack of available guidance. A long-term goal of ...


Anti-Inflammatory Activity Of Phenolic And Volatile Compounds In Cranberries (Vaccinium Macrocarpon L), Katherine Moore 2018 University of Arkansas, Fayetteville

Anti-Inflammatory Activity Of Phenolic And Volatile Compounds In Cranberries (Vaccinium Macrocarpon L), Katherine Moore

Theses and Dissertations

The primary objective of this study was to compare the anti-inflammatory effects of phenolic and volatile compounds extracted from cranberries. The Griess Reagent System assay was used to measure the in vitro anti-inflammatory capabilities of cranberry phenolic and volatile extracts on RAW 264.7 mouse macrophage cells. This study tested the anti-inflammatory capabilities of the cranberry phenolic and volatile extracts before, as a preventative treatment, and after, as a means of treating pre-existing inflammation, inducing inflammation with lipopolysaccharide (LPS). All experiments were conducted in the following manner, varying only in whether treated with the extracts before or after LPS: 1 ...


Integrating Environmental And Social Sustainability Into Performance Evaluation: A Balanced Scorecard-Based Grey-Danp Approach For The Food Industry, Gazi M. Duman, Murat Taskaynatan, Elif Kongar, Kurt A. Rosentrater 2018 University of Bridgeport

Integrating Environmental And Social Sustainability Into Performance Evaluation: A Balanced Scorecard-Based Grey-Danp Approach For The Food Industry, Gazi M. Duman, Murat Taskaynatan, Elif Kongar, Kurt A. Rosentrater

Agricultural and Biosystems Engineering Publications

In addition to retaining high levels of customer satisfaction, sustainability of businesses is also heavily reliant on the efficiency of their internal and external processes. Continuous performance evaluations using key performance metrics to leverage operations are essential in maintaining a sustainable business while achieving growth objectives for revenue and profitability. Traditionally, companies have considered various financial criteria, quality characteristics, and targeted levels of service as their primary factors for performance evaluation. However, increasing environmental and social awareness and accompanying governmental legislations are now requiring companies to integrate these two aspects into their performance evaluations. With this motivation, this study proposes ...


Whole Cell Cross-Linking To Discover Host-Microbe Protein Cognate Receptor/Ligand Pairs, Bart C. Weimer, Poyin Chen, Prerak T. Desai, Dong Chen, Jigna Shah 2018 University of California, Davis

Whole Cell Cross-Linking To Discover Host-Microbe Protein Cognate Receptor/Ligand Pairs, Bart C. Weimer, Poyin Chen, Prerak T. Desai, Dong Chen, Jigna Shah

Biological Engineering Faculty Publications

Bacterial surface ligands mediate interactions with the host cell during association that determines the specific outcome for the host–microbe association. The association begins with receptors on the host cell binding ligands on the microbial cell to form a partnership that initiates responses in both cells. Methods to determine the specific cognate partnerships are lacking. Determining these molecular interactions between the host and microbial surfaces are difficult, yet crucial in defining biologically important events that are triggered during association of the microbiome, and critical in defining the initiating signal from the host membrane that results in pathology or commensal association ...


Techno-Economic Analysis (Tea) Of Extruded Aquafeeds, Rashid Suleiman, Kurt A. Rosentrater 2018 Sokoine University of Agriculture

Techno-Economic Analysis (Tea) Of Extruded Aquafeeds, Rashid Suleiman, Kurt A. Rosentrater

Agricultural and Biosystems Engineering Publications

The worldwide decline and overexploitation of ocean fisheries stocks had provided an incentive for the rapid growth of aquaculture. The aquaculture industry has been recognized as the fastest-growing food production system globally, with a 10% increase in production per year and is one of the most reliable and sustainable growth markets for manufactured feeds. Extrusion technology has been extensively used in the modern aquatic feed manufacturing, due to nutritional, physical properties improvements and cost effectiveness of feeds. Cost related to aquatic feed remains the biggest challenge, especially for small-scale producers. In order to understand costs and potential breakeven points, a ...


Influences Of Spiciness And Visual Color Cue On Salty Taste Intensity Perception In Reduced-Sodium Cheese Dips, Valentina Rosasco Silva 2018 Louisiana State University

Influences Of Spiciness And Visual Color Cue On Salty Taste Intensity Perception In Reduced-Sodium Cheese Dips, Valentina Rosasco Silva

LSU Master's Theses

The current high levels of dietary sodium intake are negatively affecting consumers’ health. Reducing sodium consumption to the recommended amount could help prevent around 2.5 million deaths yearly and save up to $18 billion dollars in health care costs worldwide. The main objective of this research was to evaluate the possibility of increasing the saltiness perception and acceptability of reduced-sodium cheese dips by using the multisensory approach and analyzing gender differences towards salty taste expectations. Two experiments were conducted: I) The combined effects of gender and visual color cue on salty taste expectations, liking, and purchase intent (PI) of ...


Food Waste Posing A Serious Threat To Sustainability In The Kingdom Of Saudi Arabia – A Systematic Review, Mirza B. Baig, Khodran H. Al-Zahrani, Felicitas Schneider, Gary S. Straquadine, Marie Mourad 2018 King Saud University

Food Waste Posing A Serious Threat To Sustainability In The Kingdom Of Saudi Arabia – A Systematic Review, Mirza B. Baig, Khodran H. Al-Zahrani, Felicitas Schneider, Gary S. Straquadine, Marie Mourad

Applied Sciences, Technology and Education Faculty Publications

Worldwide, food waste is one of the prime issues threatening food security and the Kingdom of Saudi Arabia (KSA) is not an exception. With 427 kg of food wasted per capita per year, the country ranks among the top food wasters. Ironically, the Kingdom has limited arable lands and scarce water resources to support mass-scale agriculture and to feed its increasing population, KSA relies heavily on imports and subsidized food to meet needs. Yet, food is wasted at restaurants, caterers, cafeterias and, especially, by households such that food waste is the single-largest component of the landfills. The review article is ...


Potential Mechanisms For Nns-Induced Metabolic Deviances: Satiety Hormone Secretion And Alterations In The Gut Microbiota, Kyle J. Decker 2018 Rosalind Franklin University of Medicine and Science

Potential Mechanisms For Nns-Induced Metabolic Deviances: Satiety Hormone Secretion And Alterations In The Gut Microbiota, Kyle J. Decker

DePaul Discoveries

Rising rates of obesity and type 2 diabetes have prompted the usage and recommendation of nonnutritive sweeteners (NNS) as harmless sugar substitutes in attempts to decrease caloric intake. Contrary to the common belief that NNS remain physiologically inert post-consumption, evidence highlights their ability to alter metabolic processes via interactions in the gastrointestinal tract. An extensive review was conducted on the potential NNS-induced metabolic deviances by way of two non-mutually exclusive mechanisms. One possible mechanism involves their ability (or inability) to induce the secretion of GLP-1, a hormone produced in the gut that promotes satiety and accelerates glucose-dependent insulin secretion by ...


Integration Of Complementary And Alternative Medicine Into The Healthcare System In The United States, Nikki Lu 2018 University of Washington – Tacoma

Integration Of Complementary And Alternative Medicine Into The Healthcare System In The United States, Nikki Lu

Global Honors Theses

Chronic diseases are a prevalent issue around the world and chronic diseases are hard to prevent due to various systemic factors in the healthcare system. This paper mainly focused on socioeconomic issues and highlighted a few systemic factors in the US healthcare system. These factors have created various health disparities, inequities among socially constructed groups, and financial expenditures in the US healthcare system. Socioeconomic factors significantly impact the health and healthcare among socially constructed groups. Additionally, in this paper there are current approaches in addressing these healthcare factors such as social determinants of health and precision medicine as well as ...


Inefficient Metabolism Of The Human Milk Oligosaccharides Lacto-N-Tetraose And Lacto-N-Neotetraose Shifts Bifidobacterium Longum Subsp. Infantis Physiology, Ezgi Ozcan, David Sela 2018 University of Massachusetts, Amherst

Inefficient Metabolism Of The Human Milk Oligosaccharides Lacto-N-Tetraose And Lacto-N-Neotetraose Shifts Bifidobacterium Longum Subsp. Infantis Physiology, Ezgi Ozcan, David Sela

Open Access Articles

Human milk contains a high concentration of indigestible oligosaccharides, which likely mediated the coevolution of the nursing infant with its gut microbiome. Specifically, Bifidobacterium longum subsp. infantis (B. infantis) often colonizes the infant gut and utilizes these human milk oligosaccharides (HMOs) to enrich their abundance. In this study, the physiology and mechanisms underlying B. infantis utilization of two HMO isomers lacto-N-tetraose (LNT) and lacto-N-neotetraose (LNnT) was investigated in addition to their carbohydrate constituents. Both LNT and LNnT utilization induced a significant shift in the ratio of secreted acetate to lactate (1.7-2.0) in contrast to the catabolism of their ...


Radical Social Ecology As Deep Pragmatism: A Call To The Abolition Of Systemic Dissonance And The Minimization Of Entropic Chaos, Arielle Brender 2018 Fordham University

Radical Social Ecology As Deep Pragmatism: A Call To The Abolition Of Systemic Dissonance And The Minimization Of Entropic Chaos, Arielle Brender

Student Theses 2015-Present

This paper aims to shed light on the dissonance caused by the superimposition of Dominant Human Systems on Natural Systems. I highlight the synthetic nature of Dominant Human Systems as egoic and linguistic phenomenon manufactured by a mere portion of the human population, which renders them inherently oppressive unto peoples and landscapes whose wisdom were barred from the design process. In pursuing a radical pragmatic approach to mending the simultaneous oppression and destruction of the human being and the earth, I highlight the necessity of minimizing entropic chaos caused by excess energy expenditure, an essential feature of systems that aim ...


Impact Of Ultrasonication On Physicochemical Properties And Digestibility Of Sorghum Starch, Zhihong Wang 2018 University of Tennessee, Knoxville

Impact Of Ultrasonication On Physicochemical Properties And Digestibility Of Sorghum Starch, Zhihong Wang

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Sorghum, a highly nutritious grain, is usually not considered as a main staple in many families because it is not as easy to be digested as other grains such as rice or maize. But recent studies of ultrasonication shed a light on potential utilization of this world’s fifth important cereal other than being raw material source of biofuel or liquor. This experiment was meant to compare the differences between unsonicated and sonicated sorghum starch in terms of their physical chemical properties and digestion ability. Despite their similarities in shapes under microscopes and their thermal properties, but sonicated sorghum starch ...


Bg-4, A Bioactive Peptide From Momordica Charantia, Promotes Apoptosis In Ovarian Cancer Cells, Ashley D. Bloom 2018 University of Tennessee, Knoxville

Bg-4, A Bioactive Peptide From Momordica Charantia, Promotes Apoptosis In Ovarian Cancer Cells, Ashley D. Bloom

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Momordica charantia is a perennial plant with reported health benefits. Food-derived molecules, like the novel peptide BG-4 found in Momordica charantia, have been shown to have anticancer properties by promoting apoptosis in colon cancer cells. Ovarian cancer (OVCA) is the deadliest form of all gynecological cancers. The high fatality rate of OVCA is due to late presentation of the disease, cancer persistence, and recurrence in patients. The objective of this study was to determine the ability of BG-4 to cause cytotoxicity to ovarian cancer cells (A27801AP and COV318) and determine the mechanism involved by measuring proteins associated with apoptosis. BG-4 ...


Extraction And Purification Of (E)-Resveratrol From The Bark Of Maine's Native Spruces, Panduka S. Piyaratne 2018 University of Maine

Extraction And Purification Of (E)-Resveratrol From The Bark Of Maine's Native Spruces, Panduka S. Piyaratne

Electronic Theses and Dissertations

Resveratrol is a plant secondary metabolite which protects plants from pathogenic invasions and acts as a remedial agent against injury, stress, and tissue damage by UV radiation. With its characteristic stilbene structure, resveratrol adopts (E) and (Z) isomeric forms. However, the (E) isomer is the most biologically active and abundant form of resveratrol in nature.

(E)- Resveratrol confers a variety of benefits to human health and well-being. Besides its antioxidant activity, the role of (E)-resveratrol as an anti-inflammatory, anti-aging, cardioprotection, and anticancer agent has been a widely explored research interest. Many plant species including mulberry, peanut, and grape are ...


Effect Of Processing On In-Vitro Protein Digestibility And Other Nutritional Aspects Of Nebraska Crops, Paridhi Gulati 2018 University of Nebraska - Lincoln

Effect Of Processing On In-Vitro Protein Digestibility And Other Nutritional Aspects Of Nebraska Crops, Paridhi Gulati

Dissertations, Theses, & Student Research in Food Science and Technology

Among plant based agricultural products, Nebraska ranks first nationwide in the production of Great Northern beans (GNB), second in proso millet production, and eighth in production of winter wheat. The present research was focused on the effect of processing on nutritive components and in vitroprotein digestibility of these crops with the aim of promoting their human consumption. Proso millet based extrudates had physical properties similar to commonly extruded rice but had lower expansion than corn. GNB extrudates had limited expansion and high bulk density mostly due to high fiber and protein content. Extrusion significantly reduced the anti-nutritional components in ...


College Students’ Understandings Of, Perceptions Towards, And Usage Of Canned Foods Based On Exposure To Canned Foods During Childhood, Robert H. Drury, Lora Beth Brown, Pauline Williams, Dennis Eggett, Rickelle Richards 2018 Brigham Young University - Provo

College Students’ Understandings Of, Perceptions Towards, And Usage Of Canned Foods Based On Exposure To Canned Foods During Childhood, Robert H. Drury, Lora Beth Brown, Pauline Williams, Dennis Eggett, Rickelle Richards

Undergraduate Honors Theses

ABSTRACT

Objective: To evaluate if frequent usage of canned foods in childhood households affected college students’ current understandings of, perceptions towards, and usage of canned foods.

Methods: A secondary data analysis was conducted based on data collected from students who completed The Perceptions and Use of Canned Foods questionnaire. ANOVA statistics were used to make comparisons between students exposed (ECF; n=65) and not exposed (NECF; n=237) to canned foods during childhood.

Results: Compared with NECF, the ECF more strongly agreed that canned foods counted towards United States’ dietary recommendations (p

Conclusions and Implications: Frequent usage of canned foods ...


Effects Of Mitochondrial Nadp+-Dependent Isocitrate Dehydrogenase Deficiency On Fructose-Induced Obesity In Mice, Allison Michelle Montalbano, Kaleigh Elizabeth Beane 2018 University of Arkansas, Fayetteville

Effects Of Mitochondrial Nadp+-Dependent Isocitrate Dehydrogenase Deficiency On Fructose-Induced Obesity In Mice, Allison Michelle Montalbano, Kaleigh Elizabeth Beane

Human Nutrition and Hospitality Innovation Undergraduate Honors Theses

Obesity prevalence in the United States continues to increase and is associated with health consequences such as type 2 diabetes, hypertension, atherosclerosis, and hyperlipidemia. Among many contributing factors to obesity, fructose may be one of the major reasons as it disrupts the antioxidant system thereby resulting in an accumulation of reactive oxidative species and leading to obese conditions. The enzyme, isocitrate dehydrogenase 2 (IDH2), reduces nicotinamide adenine dinucleotide phosphate from the TCA Cycle, hence might be implicated with not only energy metabolism but also cellular redox homeostasis. Therefore, the hypothesis was that IDH2 deficiency in mice would exacerbate hepatic lipid ...


The Effect Of Breakfast On Energy Metabolism, Appetite, And Food Intake In Young Versus Older Men, Allie Murphy 2018 University of Arkansas, Fayetteville

The Effect Of Breakfast On Energy Metabolism, Appetite, And Food Intake In Young Versus Older Men, Allie Murphy

Human Nutrition and Hospitality Innovation Undergraduate Honors Theses

As life expectancy continues to rise, it is important to consider optimal nutrition recommendations to improve health outcomes, quality of life, and physical independence in older adults. Previous studies have focused on the impact of breakfast on postprandial appetite in younger adults. Research on the effects of breakfast on appetite in older adults is limited. Several studies have identified protein as a key nutrient for older adults. Protein has been shown to increase satiety for longer periods of time and decrease cravings later in the day when compared to high carbohydrate meals. However, more research is needed to determine the ...


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