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Laboratory and Basic Science Research Commons

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Relationship Between Perceived And Actual Exertion And Enjoyment Of Exercise In Individuals With Autism, Nicole Fiscella 2018 James Madison University

Relationship Between Perceived And Actual Exertion And Enjoyment Of Exercise In Individuals With Autism, Nicole Fiscella

Masters Theses, 2010-2019

Fiscella, N.A., Moran, T.E., Wigglesworth, J.K., and McKay, C.A. Relationship between Perceived and Actual Exertion and Enjoyment of Exercise in Individuals with Autism. Purpose: The purpose of this study was to examine the relationship between perceived exertion, actual exertion and enjoyment of exercise in individuals with Autism Spectrum Disorder (ASD). Methods: A total of 16 participants (12 males and 4 females) between the ages of 5 and 38 who were diagnosed with ASD participated in the study. The intervention lasted 10 weeks and consisted of participation in one of James Madison University’s Overcoming Barriers hour ...


Literature Review And Proposal: Yoga As Group Exercise Involving Oxytocin Release For Positive Mood Improvement, Rachel Fenton 2018 Bowling Green State University

Literature Review And Proposal: Yoga As Group Exercise Involving Oxytocin Release For Positive Mood Improvement, Rachel Fenton

Honors Projects

A literature review discusses yoga and health involving oxytocin creates the premise for a proposal combining the knowledge of yoga and its health benefits along with oxytocin's potential involvement during group exercise, yoga specifically. The proposed study's results of oxytocin measures and questionnaires have the potential to develop an understanding of the possible impacts of yoga on mood, particularly relationships between group exercise and yoga, which may help develop forms of group exercise or implement group yoga to assist or replace treatment for stress-caused or stress-related disorders.


The Ush2a Gene: An Analysis Of Ultrasonic Vocalizations In A Mouse Model Of Usher Syndrome Type 2, Kiana R. Akhundzadeh 2018 University of Connecticut

The Ush2a Gene: An Analysis Of Ultrasonic Vocalizations In A Mouse Model Of Usher Syndrome Type 2, Kiana R. Akhundzadeh

Honors Scholar Theses

Usher syndrome type 2 is a complex autosomal recessive genetic disorder that is characterized by moderate to severe congenital sensorineural hearing loss, the onset of retinitis pigmentosa in the second decade of life, and in some cases, vestibular dysfunction. Mutations in the USH2A gene account for 85% of cases of type 2. The USH2A gene is responsible for encoding the protein usherin, which has an important role in the development and function of inner ear hair cells and retinal photoreceptors. Until recently, it has been believed that carriers of the USH2A mutation were phenotype free. However, recent data has suggested ...


Identification And Utility Of Dna In Exosomes, Paul Kurywchak 2018 The University of Texas M D Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences

Identification And Utility Of Dna In Exosomes, Paul Kurywchak

The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences Dissertations and Theses (Open Access)

Cancer-associated mortality has been declining for two decades but remains a significant public health problem, especially when patients initially present with advanced disease. Early detection methods have improved survival rates but remain unavailable for a majority of cancers due to a lack of sensitive biomarkers or numerous limitations associated with current diagnosis strategies. Approaches to develop “liquid biopsies” by detecting tumor cells or DNA in the blood have led to several breakthroughs and create the potential for non-invasive, routine assessment of diseases status. However, these biomarkers are rare and currently difficult to isolate, especially in the early stages of disease ...


Different Methodologies To Characterize And Diagnose Sickle Cell Disease In Both Developed And Developing Nations, Mohammed AlHarbi 2018 Dominican University of California

Different Methodologies To Characterize And Diagnose Sickle Cell Disease In Both Developed And Developing Nations, Mohammed Alharbi

Graduate Master's Theses, Capstones, and Culminating Projects

Sickle cell disease (SCD) is a genetic blood disorder that causes the RBC to become sickle shaped due to a mutation in the β-globin gene encoding the protein hemoglobin. This disease causes reduced oxygen carrying capacity of RBC resulting in painful crisis, hemolytic anemia, and infection susceptibility. SCD affects around 100,000 individuals in USA alone and 14 million people globally. SCD affected individuals have high mortality rates. Early detection and constant monitoring of this disease is essential. The following review focuses on various methodologies that have emerged in the diagnosis of SCD. Also, low cost methods that can be ...


Effect Of An Arginine-To-Isoleucine Active Site Mutation On Escherichia Coli Malate Dehydrogenase Enzymatic Activity, Jon Zatorski, Bruce J. Heyen 2018 Olivet Nazarene University

Effect Of An Arginine-To-Isoleucine Active Site Mutation On Escherichia Coli Malate Dehydrogenase Enzymatic Activity, Jon Zatorski, Bruce J. Heyen

Scholar Week 2016 - present

Citric acid cycle enzymes function in an environment with numerous substrate analogues and therefore contain active site residue organizations that confer high substrate specificity. Extensive research into the catalytic mechanism of Escherichia coli malate dehydrogenase (eMDH) has identified arginine81 as being crucial to catalysis. In this investigation, an engineered eMDH having an Ile81 rather than an Arg81 (R81I) was isolated using a hexahistadine (His6) tag. Enzymatic activity of the R81I mutant with respect to malate, lactate, and pyruvate was explored. The R81I mutant did show significant activity toward malate, but did not show significant activity toward lactate or pyruvate. Investigations ...


Paclitaxel Enhances Oncolytic Potential Of Herpes Simplex Virus Type-1 In Cancer Cells, misagh naderi 2018 Louisiana State University and Agricultural and Mechanical College

Paclitaxel Enhances Oncolytic Potential Of Herpes Simplex Virus Type-1 In Cancer Cells, Misagh Naderi

LSU Master's Theses

Taxanes are spindle poisons that bind to and stabilize microtubules resulting in mitotic arrest. Herpes simplex Typ-1 (HSV-1) virions utilize the microtubular network for intracellular transport during both virus entry and virus egress from infected cells. It has been reported previously that taxanes may synergize with oncolytic herpes simplex viruses in the treatment of experimental prostate and breast tumors in mice. Other reports have indicated that taxanes may inhibit viral replication in infected cells. In this study the previously characterized Oncolytic Herpes Simplex Virus type 1 (OSVP), which was constructed in Kousoulas lab was used in conjugation with paclitaxel (taxol ...


Assay Of The Reverse Osmosis Purified Water In The Life Science Building At Bowling Green State University, Ohio, Nicholas Mendenhall, Scott O. Rogers, Neocles B. Leontis 2018 Bowling Green State University

Assay Of The Reverse Osmosis Purified Water In The Life Science Building At Bowling Green State University, Ohio, Nicholas Mendenhall, Scott O. Rogers, Neocles B. Leontis

Honors Projects

Contaminated water sources can cause problems for scientific research and result in costly delays and failures of experiments. At Bowling Green State University, the reverse osmosis supply circulating in the Life Sciences Building has been measurably contaminated for nearly three years, corresponding to a change in servicing of the system. While servicing has been accelerated, the contamination in the system remains. The focus of this research was to identify the species of bacteria and fungi growing inside of the water system so that it might alert those servicing the system, and to begin to eliminate the contamination. Reverse osmosis water ...


Development And Application Of Aquatic Toxicology Studies For The Assessment Of Impacts Due To Chemical Stressors Using Non-Standard Indigenous Organisms, Abraham Jeffrey Smith 2018 Florida International University

Development And Application Of Aquatic Toxicology Studies For The Assessment Of Impacts Due To Chemical Stressors Using Non-Standard Indigenous Organisms, Abraham Jeffrey Smith

FIU Electronic Theses and Dissertations

Research in the multidisciplinary science of ecotoxicology is crucial to assess injuries to ecosystem resources from chemical spills or other stressors used to support environmental decision-making. Established guidelines recommend the use of non-standard native species in toxicity investigations. This work focused on the use of native species for aquatic toxicity assessment to make more relevant conclusions on the potential for adverse biological effects to occur as a result to single chemical exposures or exposures to a complex mixture like oil. We apply these studies to investigate petroleum product impacts from the Deepwater Horizon incident and concerns for metal toxicity in ...


Developing A Method For Fluorescent Antibody Tagging For Identification Of Female Cells In Mixed Forensic Samples, Reilly Price 2018 Rappahannock Community College

Developing A Method For Fluorescent Antibody Tagging For Identification Of Female Cells In Mixed Forensic Samples, Reilly Price

Student Writing

In the subject of forensic science and crime scene investigation, DNA has become more valuable than ever in providing crucial information for investigators. As the number of wrongful convictions decreases and the number of exonerations increases, DNA testing is the answer to accurately solving crimes. The purpose of this experiment was to study whether or not fluorescent tagging would be an effective method of identifying and separating male and female cells. It sought to determine if immunofluorescence can be applied to forensic science and technology. Rather than spending time sorting through the victim’s DNA in order to get to ...


Determination Of Mic And Disk Diffusion Quality Control Guidelines For Meropenem–Vaborbactam, A Novel Carbapenem/Boronic Acid Β-Lactamase Inhibitor Combination, Erik Munson, Michael D. Huband, Mariana Castanheira, Kelley A. Fedler, Robert K. Flamm 2018 Marquette University

Determination Of Mic And Disk Diffusion Quality Control Guidelines For Meropenem–Vaborbactam, A Novel Carbapenem/Boronic Acid Β-Lactamase Inhibitor Combination, Erik Munson, Michael D. Huband, Mariana Castanheira, Kelley A. Fedler, Robert K. Flamm

Clinical Lab Sciences Faculty Research and Publications

Meropenem–vaborbactam is a carbapenem/cyclic boronic acid β-lactamase inhibitor combination primarily active against Gram-negative bacilli, including those harboring class A serine carbapenemases such as Klebsiella pneumoniae carbapenemase (KPC). A Clinical and Laboratory Standards Institute M23-A4 (Tier 2) quality control study established broth microdilution and disk diffusion ranges for reference strains. Two KPC-producing K. pneumoniae ATCC strains are recommended for quality control testing.


Biochemical Analysis Of Dimethyl Suberimidate-Crosslinked Yeast Nucleosomes, Yuichi Ichikawa, Paul D. Kaufman 2018 University of Massachusetts Medical School

Biochemical Analysis Of Dimethyl Suberimidate-Crosslinked Yeast Nucleosomes, Yuichi Ichikawa, Paul D. Kaufman

Open Access Articles

Nucleosomes are the fundamental unit of eukaryotic chromosome packaging, comprised of 147 bp of DNA wrapped around two molecules of each of the core histone proteins H2A, H2B, H3, and H4. Nucleosomes are symmetrical, with one axis of symmetry centered on the homodimeric interaction between the C-termini of the H3 molecules. To explore the functional consequences of nucleosome symmetry, we designed an obligate pair of H3 heterodimers, termed H3X and H3Y, allowing us to compare cells with single or double H3 alterations. Our biochemical validation of the heterodimeric X-Y interaction included intra-nucleosomal H3 crosslinking using dimethyl suberimidate (DMS). Here, we ...


History Of The 3rs In Toxicity Testing: From Russell And Burch To 21st Century Toxicology, Martin L. Stephens, Nina S. Mak 2018 Johns Hopkins University

History Of The 3rs In Toxicity Testing: From Russell And Burch To 21st Century Toxicology, Martin L. Stephens, Nina S. Mak

Martin Stephens, PhD

Toxicity testing is a key part of the process of assessing the hazards, safety, or risk that chemicals and other substances pose to humans, animals, or the environment. Standardized methods for such testing, typically involving animals, began to emerge during the first half of the 20th century. In 1959, British scientists William Russell and Rex Burch proposed a framework for reducing, refining, or replacing animal use in toxicology and other forms of biomedical experimentation. This “3Rs” or “alternatives” approach emerged at a time of growing sensitivity to the use of animals in experimentation, and progress in its implementation has been ...


Developing Novel Therapeutics For Bacterial Lung Infections, Brandon J. Baer, Ruud Veldhuizen, Cory Yamashita 2018 University of Western Ontario

Developing Novel Therapeutics For Bacterial Lung Infections, Brandon J. Baer, Ruud Veldhuizen, Cory Yamashita

Western Research Forum

Background: Bacterial lung infections are leading causes of death worldwide. Unfortunately, increasing resistance to antibiotics and the inflammation often accompanying these infections are leading to poor outcomes despite antibiotic intervention. Complicating treatment further, the tree-like branching structure of the lung makes drug delivery to distal sites of infection difficult. Our research aims to address these challenges by developing new therapeutics and new tools to improve and assess drug delivery, bacterial killing and inflammation. Our therapy combines host defense peptides, which have been shown to kill antibiotic-resistant bacteria and down regulate inflammation, with a pulmonary vehicle, exogenous surfactant, that can improve ...


Studies Of Amino Acid Mutations In Drug Resistance Of The Smo Protein, Eunice Wintona 2018 University of Nebraska at Omaha

Studies Of Amino Acid Mutations In Drug Resistance Of The Smo Protein, Eunice Wintona

Student Research and Creative Activity Fair

Smoothened receptor (SMO) is a protein that in humans, is encoded by the SMO gene. A systemic mutation in its binding pocket helps predict the sensitivity of mutant proteins to different drugs. Known as a GPCR-like receptor, it is a component of the hedgehog signaling pathway; a pathway involved in body patterning and the regulation of adult stem cells. An uncontrolled or inappropriate activation of the Hedgehog pathway drives tumor progression in cancers and a number of birth defects. To achieve these goals, the molecular modeling software MOE was used to build small molecules and drug molecules like Vismodegib and ...


Biographical Feature: Richard B. (Tom) Thomson, Jr., Ph.D., D(Abmm), F(Aam), Erik Munson 2018 Marquette University

Biographical Feature: Richard B. (Tom) Thomson, Jr., Ph.D., D(Abmm), F(Aam), Erik Munson

Clinical Lab Sciences Faculty Research and Publications

No abstract provided.


A Standard Nomenclature For Referencing And Authentication Of Pluripotent Stem Cells, Andreas Kurtz, Stefanie Seltmann, Kelly P. Smith 2018 Charité - Universitätsmedizin Berlin

A Standard Nomenclature For Referencing And Authentication Of Pluripotent Stem Cells, Andreas Kurtz, Stefanie Seltmann, Kelly P. Smith

Open Access Articles

Unambiguous cell line authentication is essential to avoid loss of association between data and cells. The risk for loss of references increases with the rapidity that new human pluripotent stem cell (hPSC) lines are generated, exchanged, and implemented. Ideally, a single name should be used as a generally applied reference for each cell line to access and unify cell-related information across publications, cell banks, cell registries, and databases and to ensure scientific reproducibility. We discuss the needs and requirements for such a unique identifier and implement a standard nomenclature for hPSCs, which can be automatically generated and registered by the ...


Modeling Canopy Effect In The Great Lakes Cladophora Model, Ankita Bakshi 2018 Michigan Technological University

Modeling Canopy Effect In The Great Lakes Cladophora Model, Ankita Bakshi

Dissertations, Master's Theses and Master's Reports

Cladophora glomerata is a filamentous green alga native to the Great Lakes. However, its nuisance growth in phosphorus rich waters negatively affects the lakes’ aesthetic and water quality. The Great Lakes Cladophora Model (GLCM) v1, developed in 1982, was the first mechanistic model to simulate Cladophora growth basing phosphorus availability and environmental conditions followed by Cladophora Growth Model and GLCM v2. In this study, the light and temperature mediation factors for Cladophora net growth are revised as a necessary step prior to the development of a self-shading algorithm. The concept of a fixed-value, maximum achievable biomass (carrying capacity) employed in ...


Lps-Induced Inos Mrna And The Pro-Apoptotic Signaling Pathway In Leukocytes Of Fit And Unfit Males, Tiffany M. Zuniga 2018 Virginia Commonwealth University

Lps-Induced Inos Mrna And The Pro-Apoptotic Signaling Pathway In Leukocytes Of Fit And Unfit Males, Tiffany M. Zuniga

Theses and Dissertations

Overexpression of the enzyme iNOS induces apoptotic cellular death by increasing indices of pro-inflammation and oxidative stress. Aerobic physical activity has been known to have anti- inflammatory benefits and reduce oxidative stress. Purpose: Therefore, this study aimed to examine the impact of aerobic fitness on LPS-induced iNOS mRNA expression and the relationship of this expression with indices of oxidative stress, pro-inflammation and apoptosis in isolated leukocytes. Methods: Whole blood samples from aerobically fit and unfit males were stimulated with and without LPS. Thereafter, iNOS mRNA expression and MDA, TNF-α and p53 concentrations were analyzed. Results: iNOS mRNA expression levels following ...


Investigation Of Female Versus Male Firefighter Anthropometrics And Ergonomic Mobility, Meredith McQuerry 2018 Florida State University

Investigation Of Female Versus Male Firefighter Anthropometrics And Ergonomic Mobility, Meredith Mcquerry

International Textile and Apparel Association (ITAA) Annual Conference Proceedings

The purpose of this study was to assess the impact of fit on female versus male firefighter range of motion (ROM) when donning a structural turnout ensemble. Three test ensembles were evaluated: base layers (BL), turnout suit (TS), and turnout ensemble (TE). Sixteen career firefighters (10 males; 6 females) completed a user needs survey on fit and mobility restrictions, were measured in a three-dimensional body scanner, performed a static ROM protocol, and recorded ease of movement and comfort perceptions for each ensemble. Average body measurements and absolute ROM values were calculated for each test ensemble and analyzed by gender. Results ...


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