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Inconceivable: An Analysis Of Assisted Reproductive Technology For The Church, Mary Elizabeth Gresham 2020 Liberty University

Inconceivable: An Analysis Of Assisted Reproductive Technology For The Church, Mary Elizabeth Gresham

Senior Honors Theses

Infertility pushes the boundaries of emotional and physical health, which is why many couples inside and outside the church turn to Assisted Reproductive Technology (ART) for a solution. Despite what has seemed like silence from the Church, some individuals have braved the biological confusion and ethical dilemmas to evaluate the technology. Three major ethical viewpoints have emerged that each prioritize something over medical technology, namely community, order, or human dignity. This paper serves to educate pastors and church leaders on the ever-changing biology of ART as well as give voice to Christians that have spoken out on this issue. At ...


Insight On Thermal Stability Of Magnetite Magnetosomes: Implications For The Fossil Record And Biotechnology, Jefferson Cypriano, Mounib Bahri, Kassioge Dembele, Walid Baaziz, Pedro Leao, Dennis A. Bazylinski, Fernanda Abreu, Ovidiu Ersen, Marcos Farina, Jacques Werckmann 2020 Universidade Federal do Rio de Janeiro

Insight On Thermal Stability Of Magnetite Magnetosomes: Implications For The Fossil Record And Biotechnology, Jefferson Cypriano, Mounib Bahri, Kassioge Dembele, Walid Baaziz, Pedro Leao, Dennis A. Bazylinski, Fernanda Abreu, Ovidiu Ersen, Marcos Farina, Jacques Werckmann

Life Sciences Faculty Publications

Magnetosomes are intracellular magnetic nanocrystals composed of magnetite (Fe3O4) or greigite (Fe3S4), enveloped by a lipid bilayer membrane, produced by magnetotactic bacteria. Because of the stability of these structures in certain environments after cell death and lysis, magnetosome magnetite crystals contribute to the magnetization of sediments as well as providing a fossil record of ancient microbial ecosystems. The persistence or changes of the chemical and magnetic features of magnetosomes under certain conditions in different environments are important factors in biotechnology and paleomagnetism. Here we evaluated the thermal stability of magnetosomes in a temperature range between 150 and 500 °C subjected ...


The Growing Threat Of Agroterrorism And Strategies For Agricultural Defense, Alyssa Forrest 2020 Liberty University

The Growing Threat Of Agroterrorism And Strategies For Agricultural Defense, Alyssa Forrest

Senior Honors Theses

Due to the dynamic nature of human conflict, non-traditional terror tactics have evolved to undermine the socioeconomic stability of targeted societies. Considering the landscape in which terrorists operate, emphasis on more subversive methods of biological terror have become prominent in recent decades. Agroterrorism, or the use of plant pathogens to infect a nation’s cultivated crops, is an emerging topic due to its threat to global food security and economic stability. Although emergency preparedness objectives have been enacted at national, state, and even local levels, preemptive measures can no longer remain the sole responsibility of intelligence and law enforcement agencies ...


Effective Faculty Onboarding, Larry Buckley, Andre O. Hudson 2020 Rochester Institute of Technology

Effective Faculty Onboarding, Larry Buckley, Andre O. Hudson

Academic Chairpersons Conference Proceedings

New Academic Faculty face a deluge of new information when they arrive on campus. This is in addition to the stresses associated with a new campus environment, and for many the first position requiring completely independent planning and organization. Many faculty contribute career success or failure to successful and comprehensive support from colleagues from the very start of new positions. This workshop will require and assist all participants to develop an onboarding framework document that they can employ at their respective academic units/institutions. Topics to be discussed and included in such an onboarding guide include clear articulations of (1 ...


Research And Review Of Polymethyl-Methacrylate Copolymers In The Development Of Enhanced Pharmaceutical Delivery Systems, Jake Dyess 2020 Liberty University

Research And Review Of Polymethyl-Methacrylate Copolymers In The Development Of Enhanced Pharmaceutical Delivery Systems, Jake Dyess

Senior Honors Theses

The following thesis shall primarily explore the current research regarding the implementation of modifiable microscopic and nanoscopic polymer particles as novel pharmaceutical delivery systems. Polymethacrylate-based copolymers, such as EudragitTM, are sensitive to alterations in pH levels, becoming increasingly more soluble in response to an increase in pH. The selective solubility and modifiable nature of these polymers allows for greater flexibility of treatment options for patients (Patra et al., 2017). The wide range of functionality of polymethacrylate (PMA) may provide solutions to challenges relating to the current treatment and therapy of certain conditions. Research indicates that PMA based micro- and ...


Chemoenzymatic Study Of Coa-Linked Rna In Bacteria, Krishna Sapkota 2020 The University of Southern Mississippi

Chemoenzymatic Study Of Coa-Linked Rna In Bacteria, Krishna Sapkota

Dissertations

The ability of RNA to store genetic information and to catalyze biochemical transformations led to the speculation of the existence of RNA world before the evolution of contemporary ribonucleoprotein (RNP) world. Recent discovery of RNA molecules containing metabolic cofactors including coenzyme A and its various thioesters at their 5’ end further supported the RNA world hypothesis as these CoA-linked RNA molecules could be the molecular fossils with very ancient origin. As both RNA and Coenzyme A are believed to have co-existed since last universal common ancestor (LUCA) or even before, the CoA-RNA conjugates in current biology may reveal fundamental molecular ...


Assessing Seed Handling Processes To Facilitate A Community-Engaged Approach To Regional Forest Restoration, Meredith Cobb, Michaela J. Woods, Ryan McEwan 2020 Five RIvers Metroparks

Assessing Seed Handling Processes To Facilitate A Community-Engaged Approach To Regional Forest Restoration, Meredith Cobb, Michaela J. Woods, Ryan Mcewan

Biology Faculty Publications

In the Midwestern United States, there is a strong management impetus toward reforestation to replace trees lost to due to tree mortality and to establish forests on previously cleared properties. Here, we describe a public outreach and volunteer effort that aimed to generate positive, community-engaged restoration outcomes and report on a practical experiment involving seed handling. We obtained tree seeds that were donated by members of the community to local land managers. We evaluated the size of the seeds collected and tested the influence of common stratification media (none, sand and vermiculite) and seed size on germination success of three ...


Optimization Of Xylanase Activity In The Presence Of Salts Of Different Metals For The Pulping Of Indigenous Non-Woody Raw Materials (Gossypium Arborium), Muhammad Suleman, Abu ul Hassan Faiz, Lariab Zahra Faiz 2020 Riphah International University, Faisalabad campus, Pakistan

Optimization Of Xylanase Activity In The Presence Of Salts Of Different Metals For The Pulping Of Indigenous Non-Woody Raw Materials (Gossypium Arborium), Muhammad Suleman, Abu Ul Hassan Faiz, Lariab Zahra Faiz

Journal of Bioresource Management

This study was conducted to optimize the process of degradation of agricultural wastes using xylanase by pulping. The cotton stalk was treated with enzyme and sugar with different concentration was obtained. The substrate concentration, incubation time, cofactors and activators on xylanase were investigated in the pulping and bleaching process. The study presents results that at pH 6.0 with time span (3, 6 and 9 hours) of incubation produced 56 mg/ml, 75 mg/ml and 112.5 mg/ml sugar respectively. While at pH 7.0 with time duration (3, 6 and 9 hours) of incubation 64 mg/ml ...


Effect Of Homofermentative Inoculant On Fermentation Characteristics And Nutritive Values Of Corn Silage, Nafeesa Qudsia Hanif, Iffat Tahira, Nighat Sultana, Mujahid Hasan 2020 Agripak Labs, Rawalpindi, Pakistan

Effect Of Homofermentative Inoculant On Fermentation Characteristics And Nutritive Values Of Corn Silage, Nafeesa Qudsia Hanif, Iffat Tahira, Nighat Sultana, Mujahid Hasan

Journal of Bioresource Management

An in vitro study was planned to assess the effects of a homofermentative microbial inoculant on the fermentation parameters and nutritive value of corn silage. The inoculant was applied at concentrations of 5x104cfu/g of forage (T1), 1x105cfu/g of forage (T2) 1.5x105cfu/g of forage (T3) and a negative control group (T0) without bacterial inoculant in three replicates each. At day 3, 7, 45 and 90 of the experiment individual buckets were opened to characterize the material, quick acidification, dry matter recovery, and aerobic stability of silage respectively. The temperature of the ...


Practical Applications And Future Directions Of Genetic Code Expansion: Validation Of Novel Akt1 Substrates And The Design Of A Synthetic Auxotroph Strain Of B. Subtilis, McShane M. McKenna 2020 The University of Western Ontario

Practical Applications And Future Directions Of Genetic Code Expansion: Validation Of Novel Akt1 Substrates And The Design Of A Synthetic Auxotroph Strain Of B. Subtilis, Mcshane M. Mckenna

Electronic Thesis and Dissertation Repository

In Chapter 1, site-specifically phosphorylated variants of the oncogene Akt1 were made in Escherichia coli using the orthogonal translation system that enable genetic code expansion with phosphoserine. The differentially phosphorylated variants of Akt1 were used to validate newly predicted Akt1 substrates. The predicted target sites of the peptide substrates were synthesized and subjected to in vitro kinase assays to quantify the activity of each Akt1 phosphorylated variant towards the predicted peptide. A previously uncharacterized kinase-substrate interaction between Akt1 and a peptide derived from RAB11 Family Interacting Protein 2 (RAB11FIP2) was validated in vitro. Chapter 2 describes the preliminary development of ...


Crispr-Cas12a Has Widespread Off-Target And Dsdna-Nicking Effects, Karthik Murugan, Arun S. Seetharam, Andrew J. Severin, Dipali G. Sashital 2020 Iowa State University

Crispr-Cas12a Has Widespread Off-Target And Dsdna-Nicking Effects, Karthik Murugan, Arun S. Seetharam, Andrew J. Severin, Dipali G. Sashital

Office of Biotechnology Publications

Cas12a (Cpf1) is an RNA-guided endonuclease in the bacterial type V-A CRISPR-Cas anti-phage immune system that can be repurposed for genome editing. Cas12a can bind and cut dsDNA targets with high specificity in vivo, making it an ideal candidate for expanding the arsenal of enzymes used in precise genome editing. However, this reported high specificity contradicts Cas12a’s natural role as an immune effector against rapidly evolving phages. Here, we employed high-throughput in vitro cleavage assays to determine and compare the native cleavage specificities and activities of three different natural Cas12a orthologs (FnCas12a, LbCas12a, and AsCas12a). Surprisingly, we observed pervasive ...


Transcriptome Analysis-Identified Long Noncoding Rna Crnde In Maintaining Endothelial Cell Proliferation, Migration, And Tube Formation, Matthew Moran, Xiao Cheng Zeng, Mohamed Sham Shihabudeen Haider Ali, Nishikant Wase, Nghi Nguyen, Weilong Yang, Chi Zhang, Concetta C. DiRusso, Xinghui Sun 2020 University of Nebraska - Lincoln

Transcriptome Analysis-Identified Long Noncoding Rna Crnde In Maintaining Endothelial Cell Proliferation, Migration, And Tube Formation, Matthew Moran, Xiao Cheng Zeng, Mohamed Sham Shihabudeen Haider Ali, Nishikant Wase, Nghi Nguyen, Weilong Yang, Chi Zhang, Concetta C. Dirusso, Xinghui Sun

Biochemistry -- Faculty Publications

Obesity is a leading risk factor for type-2 diabetes. Diabetes often leads to the dysregulation of angiogenesis, although the mechanism is not fully understood. Previously, long noncoding RNAs (lncRNAs) have been found to modulate angiogenesis. In this study, we asked how the expression levels of lncRNAs change in endothelial cells in response to excessive palmitic acid treatment, an obesitylike condition. Bioinformatics analysis revealed that 305 protein-coding transcripts were upregulated and 70 were downregulated, while 64 lncRNAs were upregulated and 46 were downregulated. Gene ontology and pathway analysis identified endoplasmic reticulum stress, HIF-1 signaling, and Toll-like receptor signaling as enriched after ...


Molecular Differentiation Of Astragalus Species And Varieties From The Western United States: The Chloroplast Dna Bridge Between Evolution And Molecular Systematics, Marwa Neyaz, Daniel Cook, Rebecca Creamer 2020 New Mexico State University

Molecular Differentiation Of Astragalus Species And Varieties From The Western United States: The Chloroplast Dna Bridge Between Evolution And Molecular Systematics, Marwa Neyaz, Daniel Cook, Rebecca Creamer

Poisonous Plant Research (PPR)

Locoweeds are the most widespread poisonous plant problem in the world and have been reported in the Western United States since the 1800s, causing tremendous losses in livestock. Consumption of locoweeds by grazing animals stimulates the neurological disease, locoism, characterized by weight loss, ataxia, and lack of muscular coordination. The name locoweed is used for Astragalus and Oxytropis species known to contain swainsonine, the toxic principle produced by the plant endophytic fungus Undifilum. Astragalus includes 2,500-3,000 species and many varieties that have almost identical morphological characteristics that overlap among species, leading to improper identification. Therefore, the aim of ...


Compounds For Increasing Lipid Synthesis And Storage, Concetta C. DiRusso, Nishikant Wase 2020 University of Nebraska - Lincoln

Compounds For Increasing Lipid Synthesis And Storage, Concetta C. Dirusso, Nishikant Wase

Biochemistry -- Faculty Publications

This invention relates to methods for increasing lipid production in cells. Methods of producing biofuel from cells and preparing mutraceuticals comprising lipids produced according to a method provided herein are also provided.


A Positive Feedback Loop Of Hippo- And C-Jun-Amino-Terminal Kinase Signaling Pathways Regulates Amyloid-Beta-Mediated Neurodegeneration, Madison Irwin, Meghana Tare, Aditi Singh, Oorvashi Roy Puli, Neha Gogia, Matthew Riccetti, Prajakta Deshpande, Madhuri Kango-Singh, Amit Singh 2020 University of Dayton

A Positive Feedback Loop Of Hippo- And C-Jun-Amino-Terminal Kinase Signaling Pathways Regulates Amyloid-Beta-Mediated Neurodegeneration, Madison Irwin, Meghana Tare, Aditi Singh, Oorvashi Roy Puli, Neha Gogia, Matthew Riccetti, Prajakta Deshpande, Madhuri Kango-Singh, Amit Singh

Biology Faculty Publications

Alzheimer's disease (AD, OMIM: 104300) is an age-related disorder that affects millions of people. One of the underlying causes of AD is generation of hydrophobic amyloid-beta 42 (Aβ42) peptides that accumulate to form amyloid plaques. These plaques induce oxidative stress and aberrant signaling, which result in the death of neurons and other pathologies linked to neurodegeneration. We have developed a Drosophila eye model of AD by targeted misexpression of human Aβ42 in the differentiating retinal neurons, where an accumulation of Aβ42 triggers a characteristic neurodegenerative phenotype. In a forward deficiency screen to look for genetic modifiers, we identified a ...


Bioprocessing Of Brewers' Spent Grain For Production Of Xylanopectinolytic Enzymes By Mucor Sp., Shady S. Hassan, Brijesh K. Tiwari, Gwilym A. Williams, Amit K. Jaiswal 2020 Technological University Dublin

Bioprocessing Of Brewers' Spent Grain For Production Of Xylanopectinolytic Enzymes By Mucor Sp., Shady S. Hassan, Brijesh K. Tiwari, Gwilym A. Williams, Amit K. Jaiswal

Articles

The potential of microwave and ultrasound was evaluated for the pretreatment of brewer's spent grain (BSG). Under optimal conditions of microwave and ultrasound pretreatments, reducing sugar yields per 1 g of pretreated BSG were 64.4 ± 7 mg and 39.9 ± 6 mg, respectively. Subsequently, the pretreated BSG was evaluated as a substrate for production of Xylanopectinolytic enzymes using fungi isolated from spoiled fruits. Out of twenty-nine (29) isolates recovered, Mucor sp. (AB1) isolated from Bramley apple (Malus domestica) produced xylanopectinolytic enzymes with higher specific activity, and was selected for further studies. The highest enzyme activity (137 U/g ...


Potential Use Of Biotherapeutic Bacteria To Target Colorectal Cancer-Associated Taxa, Gareth Lawrence, Maire Begley, Paul D. Cotter, Caitríona M. Guinane 2020 Department of Biological Sciences, Cork Institute of Technology, Cork T12 P928, Ireland

Potential Use Of Biotherapeutic Bacteria To Target Colorectal Cancer-Associated Taxa, Gareth Lawrence, Maire Begley, Paul D. Cotter, Caitríona M. Guinane

Department of Biological Sciences Publications

The role of the gut microbiome in human health and disease is the focus of much attention. It has been widely agreed upon that our gut bacteria play a role in host immunity, nutrient absorption, digestion, metabolism, and other key drivers of health. Furthermore, certain microbial signatures and specific taxa have also been associated with the development of diseases, such as obesity; inflammatory bowel disease; and, indeed, colorectal cancer (CRC), which is the focus of this review. By extension, such taxa represent potential therapeutic targets. In particular, the emerging human pathogen Fusobacterium nucleatum represents an important agent in CRC development ...


Development Of A Novel Highly-Sensitive Brucellosis Sensor Based On Surface Plasmon Resonance Spectroscopy, Amal Kasry, Ihab Adly, Asharf Sayour, Hossam Sayour 2020 The British University in Egypt

Development Of A Novel Highly-Sensitive Brucellosis Sensor Based On Surface Plasmon Resonance Spectroscopy, Amal Kasry, Ihab Adly, Asharf Sayour, Hossam Sayour

Nanotechnology Research Centre

Brucellosis is considered a significant health threat, it is an infectious disease caused by the bacteria Brucella, which can spread from animals to humans causing severe diseases. Through this project, we aim to develop a very highly sensitive biosensor to detect Brucella early before spreading. This sensor is based on surface plasmon resonance (SPR) technique, which is used to analyze kinetics of interaction between biomolecules. It can detect down to picomolar concentrations of some proteins.


Biotech Connector Brochure, 2020 University of Nebraska - Lincoln

Biotech Connector Brochure

Biotech Connector

The Biotech Connector represents an important opportunity to serve the economic needs of the people of Nebraska through further diversification of Nebraska's economy, and by helping forge and illuminate a pathway to jobs in the biotechnology space for Nebraska students.

The Biotech Connector is 7,700 sq. ft. of well-equipped wet-lab space located on Nebraska Innovation Campus. We provide incubation space and services to bioscience startups and high-growth biotech and research-based businesses.

Wet lab space provides aspiring startups and technology businesses with access to very expensive laboratory equipment that would be outside of the budget of most startups. Lack ...


Advancing Knowledge Management (Km) As An Ich Q10 Enabler In The Biopharmaceutical Industry, Paige Kane, Martin Lipa, Anne Greene (editor), Nuala Calnan (editor), Anne Murphy (editor) 2020 Technological University Dublin

Advancing Knowledge Management (Km) As An Ich Q10 Enabler In The Biopharmaceutical Industry, Paige Kane, Martin Lipa, Anne Greene (Editor), Nuala Calnan (Editor), Anne Murphy (Editor)

Books

A monograph based on doctoral research by the authors through the School of Chemistry & Pharmaceutical Science, Technological University Dublin, Ireland


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