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Full-Text Articles in Biotechnology

Effect Of External Energy On The Atomic, Crystalline, And Powder Characteristics Of Antimony And Bismuth Powders, Mahendra Kumar Trivedi Sep 2099

Effect Of External Energy On The Atomic, Crystalline, And Powder Characteristics Of Antimony And Bismuth Powders, Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Next to atoms and molecules the powders are the smallest state of matter available in high purities and large quantities. The effect of any external energy on the shape, morphology and structure can thus be studied with relative ease. The present investigation deals with the effect of a non-contact external energy on the powders of antimony and bismuth. The characteristics of powders treated by external energy are compared with the as received powders (control). The average particle sizes, d50 and d99, the sizes below which 99% of the particles are present showed significant increase and decrease indicating that the energy ...


Integrative Computational Approach Identifies Drug Targets In Cd4+ T-Cell-Mediated Immune Disorders, Bhanwar L. Puniya, Rada Amin, Bailee Lichter, Robert Moore, Alex Ciurej, Sydney J. Bennett, Ab Rauf Shah, Matteo Barberis, Tomáš Helikar May 2021

Integrative Computational Approach Identifies Drug Targets In Cd4+ T-Cell-Mediated Immune Disorders, Bhanwar L. Puniya, Rada Amin, Bailee Lichter, Robert Moore, Alex Ciurej, Sydney J. Bennett, Ab Rauf Shah, Matteo Barberis, Tomáš Helikar

Biochemistry -- Faculty Publications

CD4+ T cells provide adaptive immunity against pathogens and abnormal cells, and they are also associated with various immunerelated diseases. CD4+ T cells’ metabolism is dysregulated in these pathologies and represents an opportunity for drug discovery and development. Genome-scale metabolic modeling offers an opportunity to accelerate drug discovery by providing high-quality information about possible target space in the context of a modeled disease. Here, we develop genome-scale models of naïve, Th1, Th2, and Th17 CD4+ T-cell subtypes to map metabolic perturbations in rheumatoid arthritis, multiple sclerosis, and primary biliary cholangitis. We subjected these models to in silico simulations for drug ...


Agrobacterium Mediated Transformation Optimizations For Sugarcane (Saccharum Officinarum L.) Cultivar Spf-234 With Direct Organogenesis, Muhammad Nawaz, Naeem Iqbal, Rabia Hameed, Mehwish Mehwish, Shakra Jamil May 2021

Agrobacterium Mediated Transformation Optimizations For Sugarcane (Saccharum Officinarum L.) Cultivar Spf-234 With Direct Organogenesis, Muhammad Nawaz, Naeem Iqbal, Rabia Hameed, Mehwish Mehwish, Shakra Jamil

Journal of Bioresource Management

Sugarcane (Saccharum officinarum L.) is the most important food and energy crop worldwide. In the present study, an efficient Agrobacterium mediated transformation and regeneration system for sugarcane cultivar SPF-234 was established. Agrobacterium tumefaciens strains EHA101and LBA4404 using vector pIG121 Hm, having GUS, HPTII and NPTII genes were used. Polymerase chain reaction (PCR) and histochemical assays confirmed the GUS gene expression. A 620 bp fragment from GUS positive plants was amplified. The GUS expressing putative transformants were 35% of the total plants formed under 30 minute immersion time and 72 hr of incubation period. The co-cultivation media having 60 µM acetosyringone ...


Partial Expression Of The Vbss Gene In Rhizobium Leguminosarum Atcc 14479 And In-Silico Analysis Of The Vbs Gene Cluster In Various Microorganisms, Afreen Siddiqui May 2021

Partial Expression Of The Vbss Gene In Rhizobium Leguminosarum Atcc 14479 And In-Silico Analysis Of The Vbs Gene Cluster In Various Microorganisms, Afreen Siddiqui

Undergraduate Honors Theses

Iron is extremely important for many organisms. Despite its abundancy, it exists in insoluble forms that makes its usability difficult. Some organisms secrete siderophores, low molecular weight compounds, that can chelate iron and convert it into usable forms for cells. One such organism, Rhizobium leguminosarum, is a nitrogen fixing symbiont proteobacteria that infects leguminous plants. The genome of Rhizobium leguminosarum ATCC 14479, which infects the red clover, Trifoli pratense, has previously been completely sequenced in our lab. Our lab has identified several genes in this strain involved in the biosynthesis of a siderophore, vicibactin. The protein product of one of ...


Light Intensity Control For Plc-Based Hydroponic Demonstration System, Christian Hitt May 2021

Light Intensity Control For Plc-Based Hydroponic Demonstration System, Christian Hitt

Biological and Agricultural Engineering Undergraduate Honors Theses

In order to demonstrate a skill set obtained through the University of Arkansas Biological Engineering, a group of students and I developed a hydroponic system where plants could be grown in a chamber. System parameters such as air quality, irrigation, and lighting were controlled by a Programmable Logic Computer (PLC) and an array of sensors and actuators. Each student focused on a certain aspect of this system, mine being the lighting. Developing this system involved researching the relationship between light intensity and plant growth, designing a system to suspend the lights above the growth chamber, and develop a system to ...


Understanding The Genetics Of Schizophrenia, Matthew Toohey Apr 2021

Understanding The Genetics Of Schizophrenia, Matthew Toohey

Thinking Matters Symposium

Schizophrenia has been considered heritable for a long time, but only with the advent of new technologies such as whole-genome sequencing and genome-wide association studies can we begin to identify specific molecular causes of schizophrenia. This poster will review some of the genetic variants that research groups have associated with schizophrenia. Current research has indicated that schizophrenia is a polygenic disease and has been linked to many genes. Some of these common risk variants are in protein coding sections of the DNA. These proteins are often linked to neurological development or immune system function. Other variants that have been associated ...


Importance Of Understanding Genetic Predisposition, Andrew Carter Apr 2021

Importance Of Understanding Genetic Predisposition, Andrew Carter

Thinking Matters Symposium

Hypersensitivity reactions can be sudden and are often fatal. Many of these reactions occur as a result of allergies to prescribed medication. Adverse drug reactions or (ADR’s) were show in a recent study to affect over 6% of hospitalized patients and resulted in over 100,000 deaths a year in the US alone. This poster will review the importance of understanding genetic predisposition, through articles about possible genetic causes of allergies to penicillin and other beta-lactams. Changes in a specific HLA gene located on chromosome 6 showed a correlation to penicillin reactions in over 600,000 participants. Another study ...


A Pseudomolecule Assembly Of The Rocky Mountain Elk Genome, Rick E. Masonbrink, David Alt, Darrell O. Bayles, Paola Boggiatto, William Edwards, Fred Tatum, Jeffrey Williams, Jennifer Wilson-Welder, Aleksey Zimin, Andrew Severin, Steven Olsen Apr 2021

A Pseudomolecule Assembly Of The Rocky Mountain Elk Genome, Rick E. Masonbrink, David Alt, Darrell O. Bayles, Paola Boggiatto, William Edwards, Fred Tatum, Jeffrey Williams, Jennifer Wilson-Welder, Aleksey Zimin, Andrew Severin, Steven Olsen

Office of Biotechnology Publications

Rocky Mountain elk (Cervus canadensis) populations have significant economic implications to the cattle industry, as they are a major reservoir for Brucella abortus in the Greater Yellowstone area. Vaccination attempts against intracellular bacterial diseases in elk populations have not been successful due to a negligible adaptive cellular immune response. A lack of genomic resources has impeded attempts to better understand why vaccination does not induce protective immunity. To overcome this limitation, PacBio, Illumina, and Hi-C sequencing with a total of 686-fold coverage was used to assemble the elk genome into 35 pseudomolecules. A robust gene annotation was generated resulting in ...


Impact Of Krasg13d Mutation On Colorectal Cancer Cell Line Hct116 Organelle Structure, Function, And Growth, Rachel Craig, Abigail Solitro Apr 2021

Impact Of Krasg13d Mutation On Colorectal Cancer Cell Line Hct116 Organelle Structure, Function, And Growth, Rachel Craig, Abigail Solitro

Liberty University Research Week

Undergraduate

Theoretical Proposal


The Relationship Between Kras And The Endoplasmic Reticulum-Mitochondrial Calcium Flux In Isogenic Colorectal Cancer Cell Lines, Emma Anderson, Abigail Solitro Apr 2021

The Relationship Between Kras And The Endoplasmic Reticulum-Mitochondrial Calcium Flux In Isogenic Colorectal Cancer Cell Lines, Emma Anderson, Abigail Solitro

Liberty University Research Week

Undergraduate

Theoretical Proposal


B25: Generation And Characterization Of Sars-Cov-2 Spike Protein-Pseudotyped Lentivirus Particles For Use In Neutralizing Antibody Titer Testing Of Human Serum, Luis Sanchez Apr 2021

B25: Generation And Characterization Of Sars-Cov-2 Spike Protein-Pseudotyped Lentivirus Particles For Use In Neutralizing Antibody Titer Testing Of Human Serum, Luis Sanchez

Annual Research Symposium

No abstract provided.


A Multi-Institutional Partnership Catalyzing The Commercialization Of Medical Devices And Biotechnology Products., Nathaniel Hafer, Bryan Buchholz, Denise Dunlap, Brennan Fournier, Scott Latham, Mary Ann Picard, Steven Tello, Laura L. Gibson, Craig M. Lilly, David D. Mcmanus Apr 2021

A Multi-Institutional Partnership Catalyzing The Commercialization Of Medical Devices And Biotechnology Products., Nathaniel Hafer, Bryan Buchholz, Denise Dunlap, Brennan Fournier, Scott Latham, Mary Ann Picard, Steven Tello, Laura L. Gibson, Craig M. Lilly, David D. Mcmanus

COVID-19 Publications by UMMS Authors

The commercialization of medical devices and biotechnology products is characterized by high failure rates and long development lead times particularly among start-up enterprises. To increase the success rate of these high-risk ventures, the University of Massachusetts Lowell (UML) and University of Massachusetts Medical School (UMMS) partnered to create key academic support centers with programs to accelerate entrepreneurship and innovation in this industry. In 2008, UML and UMMS founded the Massachusetts Medical Device Development Center (M2D2), which is a business and technology incubator that provides business planning, product prototyping, laboratory services, access to clinical testing, and ecosystem networking to medical device ...


Development Of Miriplatin-Loaded Nanoparticles Against Non-Small Cell Lung Cancer, Zhongyue Yuan, Zizhao Xu, Xin Guo Apr 2021

Development Of Miriplatin-Loaded Nanoparticles Against Non-Small Cell Lung Cancer, Zhongyue Yuan, Zizhao Xu, Xin Guo

Graduate and Professional Student Research Showcase

Lung cancer claims the highest mortality and the second-most new cases among all oncological diseases. NSCLC accounts for approximately 85% of all newly diagnosed lung cancers. Although platinum-based drugs are standard first-line chemotherapy for stage IIIB/IV NSCLC, accumulating reports have shown the failure of conventional platinum-based regimens due to drug resistance. Miriplatin is a lipophilic anti-cancer drug that has been approved in Japan for transcatheter arterial chemoembolization treatment of hepatocellular carcinoma. Lipid-based nanoparticles such as liposomes, micelles, and solid lipid nanoparticles (SLNs) can encapsulate anti-cancer drugs to improve their water solubility and bioavailability.


Systematic In Vitro Specificity Profiling Reveals Nicking Defects In Natural And Engineered Crispr–Cas9 Variants, Karthik Murugan, Shravanti K. Suresh, Arun S. Seetharam, Andrew J. Severin, Dipali G. Sashital Mar 2021

Systematic In Vitro Specificity Profiling Reveals Nicking Defects In Natural And Engineered Crispr–Cas9 Variants, Karthik Murugan, Shravanti K. Suresh, Arun S. Seetharam, Andrew J. Severin, Dipali G. Sashital

Office of Biotechnology Publications

Cas9 is an RNA-guided endonuclease in the bacterial CRISPR–Cas immune system and a popular tool for genome editing. The commonly used Streptococcus pyogenes Cas9 (SpCas9) is relatively non-specific and prone to off-target genome editing. Other Cas9 orthologs and engineered variants of SpCas9 have been reported to be more specific. However, previous studies have focused on specificity of double-strand break (DSB) or indel formation, potentially overlooking alternative cleavage activities of these Cas9 variants. In this study, we employed in vitro cleavage assays of target libraries coupled with high-throughput sequencing to systematically compare cleavage activities and specificities of two natural Cas9 ...


Comparative Expression Studies Of Fiber Related Genes In Cotton Spp., Muhammad Nadeem, Nadia Iqbal, Ummara Waheed, Zia Ullah Zia, Muqarrab Ali, Zulqurnain Khan Mar 2021

Comparative Expression Studies Of Fiber Related Genes In Cotton Spp., Muhammad Nadeem, Nadia Iqbal, Ummara Waheed, Zia Ullah Zia, Muqarrab Ali, Zulqurnain Khan

Journal of Bioresource Management

Cotton fibers are the seed trichomes that are developed around the seed and are used to make clothes and yarn for the textile industry. Expression profiling of cotton fiber genes is very important to estimate the differential gene expression level at different fiber developmental stages. Expression analysis of fiber developing genes are very important to enhance the fiber length of cotton. The expression profiling of three gene families in five stages (0, 5, 10, 15 and 20 DPA) of cotton fiber tissues was carried out through real-time PCR. Expression analysis revealed that transcripts of GA-20 Oxidase, XTH, and PEPc ...


The Look Ahead Trace Back Optimizer For Genomic Selection Under Transparent And Opaque Simulators, Fatemeh Amini, Felipe Restrepo Franco, Guiping Hu, Lizhi Wang Feb 2021

The Look Ahead Trace Back Optimizer For Genomic Selection Under Transparent And Opaque Simulators, Fatemeh Amini, Felipe Restrepo Franco, Guiping Hu, Lizhi Wang

Industrial and Manufacturing Systems Engineering Publications

Recent advances in genomic selection (GS) have demonstrated the importance of not only the accuracy of genomic prediction but also the intelligence of selection strategies. The look ahead selection algorithm, for example, has been found to significantly outperform the widely used truncation selection approach in terms of genetic gain, thanks to its strategy of selecting breeding parents that may not necessarily be elite themselves but have the best chance of producing elite progeny in the future. This paper presents the look ahead trace back algorithm as a new variant of the look ahead approach, which introduces several improvements to further ...


Disruptive Technologies With Applications In Airline & Marine And Defense Industries, Randall K. Nichols, Hans C. Mumm, Wayne Lonstein, Suzanne Sincavage, Candice M. Carter, John-Paul Hood, Randall Mai, Mark Jackson, Bart Shields Feb 2021

Disruptive Technologies With Applications In Airline & Marine And Defense Industries, Randall K. Nichols, Hans C. Mumm, Wayne Lonstein, Suzanne Sincavage, Candice M. Carter, John-Paul Hood, Randall Mai, Mark Jackson, Bart Shields

NPP eBooks

Disruptive Technologies With Applications in Airline, Marine, Defense Industries is our fifth textbook in a series covering the world of Unmanned Vehicle Systems Applications & Operations On Air, Sea, and Land. The authors have expanded their purview beyond UAS / CUAS / UUV systems that we have written extensively about in our previous four textbooks. Our new title shows our concern for the emergence of Disruptive Technologies and how they apply to the Airline, Marine and Defense industries. Emerging technologies are technologies whose development, practical applications, or both are still largely unrealized, such that they are figuratively emerging into prominence from a background of nonexistence or obscurity. A Disruptive technology is one that displaces an established technology and shakes up the industry or a ground-breaking product that creates a completely new industry.That is what our book is about. The authors think we have found technology trends that will replace the status quo or disrupt the conventional technology paradigms.The authors have collaborated to write some explosive chapters in Book 5:Advances in Automation & Human Machine Interface; Social Media as a Battleground in Information Warfare (IW); Robust cyber-security alterative / replacement for the popular Blockchain Algorithm and a clean solution for Ransomware; Advanced sensor technologies that are used by UUVs for munitions characterization, assessment, and classification and counter hostile use of UUVs against U.S. capital assets in the South China Seas. Challenged the status quo and debunked the climate change fraud with verifiable facts; Explodes our minds with nightmare technologies that if they ...


Mining Selected Metagenomes/Metatranscriptomes For Biosynthetic Gene Clusters And Antimicrobial Resistance Genes, Ahmed Yamany Jan 2021

Mining Selected Metagenomes/Metatranscriptomes For Biosynthetic Gene Clusters And Antimicrobial Resistance Genes, Ahmed Yamany

Theses and Dissertations

Antimicrobial resistance is one of the serious global challenges in the current century. The fact that resistance genes transfer between bacteria, coupled with the fact that the world is connected through complex dynamics. Studying microbial behavior and understanding the different factors coffering microbial resistance to a broad spectrum of the available drug classes, parallel with a comprehensive analysis of the natural microbial products as the primary source of the novel antibiotics, might shed some light on solutions for this problem. Microbial environments harbor a wide range of secondary metabolites (SM) with different functional groups. SMs are not directly involved in ...


Long Non-Coding Rna Meg3 Deficiency Impairs Glucose Homeostasis And Insulin Signaling By Inducing Cellular Senescence Of Hepatic Endothelium In Obesity, Xiao Cheng, Mohamed Sham Shihabudeen Haider Ali, Matthew Moran, Martonio Ponte Viana, Sarah L. Schlichte, Matthew C. Zimmerman, Oleh Khalimonchuk, Mark W. Feinberg, Xinghui Sun Jan 2021

Long Non-Coding Rna Meg3 Deficiency Impairs Glucose Homeostasis And Insulin Signaling By Inducing Cellular Senescence Of Hepatic Endothelium In Obesity, Xiao Cheng, Mohamed Sham Shihabudeen Haider Ali, Matthew Moran, Martonio Ponte Viana, Sarah L. Schlichte, Matthew C. Zimmerman, Oleh Khalimonchuk, Mark W. Feinberg, Xinghui Sun

Biochemistry -- Faculty Publications

Obesity-induced insulin resistance is a risk factor for diabetes and cardiovascular disease. However, the mechanisms underlying endothelial senescence in obesity, and how it impacts obesity-induced insulin resistance remain incompletely understood. In this study, transcriptome analysis revealed that the long non-coding RNA (lncRNA) Maternally expressed gene 3 (Meg3) is one of the top differentially expressed lncRNAs in the vascular endothelium in diet-induced obese mice. Meg3 knockdown induces cellular senescence of endothelial cells characterized by increased senescence-associated β–galactosidase activity, increased levels of endogenous superoxide, impaired mitochondrial structure and function, and impaired autophagy. Moreover, Meg3 knockdown causes cellular senescence of hepatic endothelium ...


Teaching Metabolism In Upper-Division Undergraduate Biochemistry Courses Using Online Computational Systems And Dynamical Models Improves Student Performance, Christine S. Booth, Changsoo Song, Michelle E. Howell, Achilles Rasquinha, Aleš Saska, Resa M. Helikar, Sharmin M. Sikich, Brain A. Couch, Karin Van Dijk, Rebecca Roston, Tomáš Helikar Jan 2021

Teaching Metabolism In Upper-Division Undergraduate Biochemistry Courses Using Online Computational Systems And Dynamical Models Improves Student Performance, Christine S. Booth, Changsoo Song, Michelle E. Howell, Achilles Rasquinha, Aleš Saska, Resa M. Helikar, Sharmin M. Sikich, Brain A. Couch, Karin Van Dijk, Rebecca Roston, Tomáš Helikar

Biochemistry -- Faculty Publications

Understanding metabolic function requires knowledge of the dynamics, interdependence, and regulation of metabolic networks. However, multiple professional societies have recognized that most undergraduate biochemistry students acquire only a surface-level understanding of metabolism. We hypothesized that guiding students through interactive computer simulations of metabolic systems would increase their ability to recognize how individual interactions between components affect the behavior of a system under different conditions. The computer simulations were designed with an interactive activity (i.e., module) that used the predict–observe–explain model of instruction to guide students through a process in which they iteratively predict outcomes, test their predictions ...


Titanium Dioxide Nanoparticles Binding To Human Blood Plasma Proteins, Morgan Miller Jan 2021

Titanium Dioxide Nanoparticles Binding To Human Blood Plasma Proteins, Morgan Miller

Williams Honors College, Honors Research Projects

Titanium Dioxide (TiO2, titania) nanoparticles are widely studied in cosmetology, food administration, and medical fields. This is due to the nanoparticles’ suitable properties, specific combination of strength and high corrosion resistance. With the increasing attention to these particles, there is concern with potential health complications. Some of the complications involved with titania nanoparticles include blood clots and immune reactions (Sit et al., 2019). In this study, we continue the study conducted in the Sahai research group (J. Chen. MS thesis, 2020), on the binding of titania nanoparticles with blood plasma proteins, focusing on potential effects of shape only. Hydrophobicity ...


Identification And Characterisation Of Putative Drug Binding Sites In Human Atp-Binding Cassette B5 (Abcb5) Transporter, Lokeswari P. Tangella, Mahreen Arooj, Evelyne Deplazes, Elin S. Gray, Ricardo L. Mancera Jan 2021

Identification And Characterisation Of Putative Drug Binding Sites In Human Atp-Binding Cassette B5 (Abcb5) Transporter, Lokeswari P. Tangella, Mahreen Arooj, Evelyne Deplazes, Elin S. Gray, Ricardo L. Mancera

ECU Publications Post 2013

© 2020 The Author(s) The human ATP-binding cassette B5 (ABCB5) transporter, a member of the ABC transporter superfamily, is linked to chemoresistance in tumour cells by drug effluxion. However, little is known about its structure and drug-binding sites. In this study, we generated an atomistic model of the full-length human ABCB5 transporter with the highest quality using the X-ray crystal structure of mouse ABCB1 (Pgp1), a close homologue of ABCB5 and a well-studied member of the ABC family. Molecular dynamics simulations were used to validate the atomistic model of ABCB5 and characterise its structural properties in model cell membranes. Molecular ...


Origami-Inspired Approaches For Biomedical Applications, Abdor Rahman Ahmed, Olivia C. Gauntlett, Gulden Camci-Unal Dec 2020

Origami-Inspired Approaches For Biomedical Applications, Abdor Rahman Ahmed, Olivia C. Gauntlett, Gulden Camci-Unal

Open Access Publications by UMMS Authors

Modern day biomedical applications require progressions that combine advanced technology with the conformability of naturally occurring, complex biosystems. These advancements yield conformational interactions between the biomedical devices and the biological organisms' structures. Biomedical applications that adapt origami-inspired approaches have accrued aspired advances. Along with application-specific advantages, the most pertinent advances provided by origami-inspired strategies include voluminous structures with the ability to conform to biosystems, shape-shifting from two-dimensional (2D) to three-dimensional (3D) structures, and biocompatibility. Throughout this paper, the exploration of new studies, primarily within the past decade, with origami-based applications of biomedical devices, including their theories, experimental results, and plans ...


Controlled Membrane Remodeling By Nanospheres And Nanorods: Experiments Targeting The Design Principles For Membrane-Based Materials, Sarah Zuraw-Weston Dec 2020

Controlled Membrane Remodeling By Nanospheres And Nanorods: Experiments Targeting The Design Principles For Membrane-Based Materials, Sarah Zuraw-Weston

Doctoral Dissertations

In this thesis we explore two experimental systems probing the interactions of nanoparticles with lipid bilayer membranes. Inspired by the ability of cell membranes to alter their shape in response to bound particles, we report two experimental studies: one of nanospheres the other of long, slender nano-rods binding to lipid bilayer vesicles and altering the membrane shape. Our work illuminates the role of particle geometry, particle concentration, adhesion strength and membrane tension in how membrane morphology is determined. We combine giant unilamellar vesicles with oppositely charged nanoparticles, carefully tuning adhesion strength, membrane tension and particle concentration.

In the case of ...


Micro-Physiological Models To Mimic Mucosal Barrier Complexity Of The Human Intestine In Vitro, Abhinav Sharma Dec 2020

Micro-Physiological Models To Mimic Mucosal Barrier Complexity Of The Human Intestine In Vitro, Abhinav Sharma

Doctoral Dissertations

The mucosal barrier in the intestine is vital to maintain selective absorption of nutrients while protecting internal tissues and maintaining symbiotic relationship with luminal microbiota. This bio-barrier consists of a cellular epithelial barrier and an acellular mucus barrier. Secreted mucus regulates barrier function via in situ biochemical and biophysical interaction with luminal content that continually evolves during digestion and absorption. Increasing evidence suggests that a mucus barrier is indispensable to maintain homeostasis in the gastrointestinal tract. However, the importance of mucus barrier is largely underrated for in vitro mucosal tissue modeling. The major gap is the lack of experimental material ...


Industrial Biotechnology—An Industry At An Inflection Point, Nathan Danielson, Sarah Mckay, Paul Bloom, Jennifer Dunn, Neal Jakel, Timothy Bauer, John Hannon, Michael C. Jewett, Brent H. Shanks Dec 2020

Industrial Biotechnology—An Industry At An Inflection Point, Nathan Danielson, Sarah Mckay, Paul Bloom, Jennifer Dunn, Neal Jakel, Timothy Bauer, John Hannon, Michael C. Jewett, Brent H. Shanks

Chemical and Biological Engineering Publications

Industrial biotechnology is poised for dramatic growth. A confluence of consumer demand; attractive feedstock quantity, quality, and price; and technical innovation has created a perfect situation for the industry to significantly expand. Since the 2004 Werpy and Peterson paper, “Top Value Added Chemicals from Biomass,” the biobased manufacturing industry and the technologies that make these processes and products possible has evolved significantly.1 New technology continues to advance, and there is increasing consumer and industry demand for biobased materials. Paired with increasing on-farm efficiency and sustainability considerations, these are all factors that are driving the bioeconomy forward. This paper reviews ...


Molecular Identification, Antioxidant Efficacy Of Phenolic Compounds, And Antimicrobial Activity Of Beta-Carotene Isolated From Fruiting Bodies Of Suillus Sp, Shimal Yonuis Abdulhadi, Raghad Nawaf Gergees, Ghazwan Qasim Hasan Dec 2020

Molecular Identification, Antioxidant Efficacy Of Phenolic Compounds, And Antimicrobial Activity Of Beta-Carotene Isolated From Fruiting Bodies Of Suillus Sp, Shimal Yonuis Abdulhadi, Raghad Nawaf Gergees, Ghazwan Qasim Hasan

Karbala International Journal of Modern Science

Suillus species, in general, are edible mushrooms, and environmentally important that are associated mostly with pine trees in the tropics regions. These fungi considered a remarkable source of phenolic compounds that play a crucial role as antioxidants which may reduce the risk of most human chronic diseases such as cancer, diabetes, asthma, atherosclerosis, Alzheimer, and others. On the other hand, carotenoids (β carotene) are the most popular natural pigments which play an important role to protect the plants from photo-oxidative reactions. In human, these compounds prevent oxidative stress and expects to have antimicrobial activity. Here, the phenolic compounds were extracted ...


Carbon Dots As Artificial Peroxidases For Analytical Applications, Shih-Chun Wei, Yang-Wei Lin, Huan-Tsung Chang Dec 2020

Carbon Dots As Artificial Peroxidases For Analytical Applications, Shih-Chun Wei, Yang-Wei Lin, Huan-Tsung Chang

Journal of Food and Drug Analysis

Nanozymes have become attractive in analytical and biomedical fields, mainly because of their low cost, long shelf life, and less environmental sensitivity. Particularly, nanozymes formed from nanomaterials having high surface area and rich active sites are interesting since their activities can be tuned through carefully controlling their size, morphology, and surface properties. This review article focuses on preparation of carbon dots (C dots) possessing peroxidase-like activity and their analytical applications. We highlight the important roles of the oxidation states and surface residues of C dots and their nanocomposites with metal, metal oxides, or metal sulfides playing on determining their specificity ...


Antibacterial Activity Of Selected Plant Extracts On Methicillin Resistant Staphylococcus Aureus, Lubna Abdallah Nov 2020

Antibacterial Activity Of Selected Plant Extracts On Methicillin Resistant Staphylococcus Aureus, Lubna Abdallah

Journal of the Arab American University مجلة الجامعة العربية الامريكية للبحوث

Based on the importance of herbal plants in medicine, this study was conducted to evaluate the antibacterial efficacy of aqueous, ethanol and methanol extracts of Thymbra spicata, Nepeta curviflora, and Verbascum fruticulosum against two clinical isolates (I and II) of the gram positive methicillin resistant Staphylococcus aureus (MRSA). Agar well diffusion method was performed to examine the antibacterial activity of all studied plant extracts. Micro-broth dilution method was used to measure the minimum inhibitory concentration (MIC) then the minimum bacteriocidal concentration (MBC) for all active extracts. The examined isolates were susceptible to the prepared plant extracts. Ethanol and methanol extracts ...


In Vitro Invistigation Of The Heterodimerization Between Angiotensin Ii And Thrombin Receptors, Isra Mansur Al Zamel Nov 2020

In Vitro Invistigation Of The Heterodimerization Between Angiotensin Ii And Thrombin Receptors, Isra Mansur Al Zamel

Biology Theses

The renin angiotensin system (RAS) through its hormone angiotensin (AngII) and the protease thrombin are two major physiological regulators of vascular and renal functions. RAS is involved in the regulation of blood pressure and water-electrolytes hemostasis, while thrombin has a crucial role in platelets activation and thrombosis. The interplay between these two systems at the physiological and the pathophysiological levels has been documented in many studies. However, such an interplay at the pharmacological level between the AngII and thrombin receptors still has not been explored. Our study aimed to explore the interplay between the AngII type 1 receptor (AT1R) and ...