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Predation On Stink Bugs (Hemiptera: Pentatomidae) In Cotton And Soybean Agroecosystems [Predator Capture Numbers], Kacie J. Athey, John R. Ruberson, Dawn Olson, James D. Harwood 2019 University of Kentucky

Predation On Stink Bugs (Hemiptera: Pentatomidae) In Cotton And Soybean Agroecosystems [Predator Capture Numbers], Kacie J. Athey, John R. Ruberson, Dawn Olson, James D. Harwood

Entomology Research Data

Stink bugs (Hemiptera: Pentatomidae) are significant pests of cotton and soybeans in the southeastern United States with annual control costs exceeding $14 million in these crops. Three of the most prominent stink bug pests are the southern green (Nezara viridula), brown (Euschistus servus) and green (Chinavia hilaris) stink bugs. To determine trophic linkages between generalist arthropod predators and these pests, species-specific 16S molecular markers were designed and used to detect the presence of prey DNA in predator gut-contents. Over 2700 predators were collected over two growing seasons in cotton and soybean in southern Georgia in 2011 and 2012 and screened ...


Rna Sequencing, Selection Of Reference Genes And Demonstration Of Feeding Rnai In Thrips Tabaci (Lind.) (Thysanoptera: Thripidae), Satnam Singh, Mridula Gupta, Suneet Pandher, Gurmeet Kaur, Neha Goel, Pankaj Rathore, Subba Reddy Palli 2019 Punjab Agricultural University, India

Rna Sequencing, Selection Of Reference Genes And Demonstration Of Feeding Rnai In Thrips Tabaci (Lind.) (Thysanoptera: Thripidae), Satnam Singh, Mridula Gupta, Suneet Pandher, Gurmeet Kaur, Neha Goel, Pankaj Rathore, Subba Reddy Palli

Entomology Faculty Publications

Background: Thrips tabaci is a severe pest of onion and cotton. Due to lack of information on its genome or transcriptome, not much is known about this insect at the molecular level. To initiate molecular studies in this insect, RNA was sequenced; de novo transcriptome assembly and analysis were performed. The RNAseq data was used to identify reference and RNAi pathway genes in this insect. Additionally, feeding RNAi was demonstrated in T. tabaci for the first time.

Results: From the assembled transcriptome, 27,836 coding sequence (CDS) with an average size of 1236 bp per CDS were identified. About 85 ...


Fifty Years Of Cave Arthropod Sampling: Techniques And Best Practices, J. Judson Wynne, Francis G. Howarth, Stefan Sommer, Brett G. Dickson 2019 Northern Arizona University

Fifty Years Of Cave Arthropod Sampling: Techniques And Best Practices, J. Judson Wynne, Francis G. Howarth, Stefan Sommer, Brett G. Dickson

International Journal of Speleology

Ever-increasing human pressures on cave biodiversity have amplified the need for systematic, repeatable, and intensive surveys of cave-dwelling arthropods to formulate evidence-based management decisions. We examined 110 papers (from 1967 to 2018) to: (i) understand how cave-dwelling invertebrates have been sampled; (ii) provide a summary of techniques most commonly applied and appropriateness of these techniques, and; (iii) make recommendations for sampling design improvement. Of the studies reviewed, over half (56) were biological inventories, 43 ecologically focused, seven were techniques papers, and four were conservation studies. Nearly one-half (48) of the papers applied systematic techniques. Few papers (24) provided enough information ...


Interacting Stressors Matter: Diet Quality And Virus Infection In Honeybee Health, Adam G. Dolezal, Jimena Carrillo-Tripp, Timothy M. Judd, W. Allen Miller, Bryony C. Bonning, Amy L. Toth 2019 University of Illinois at Urbana-Champaign

Interacting Stressors Matter: Diet Quality And Virus Infection In Honeybee Health, Adam G. Dolezal, Jimena Carrillo-Tripp, Timothy M. Judd, W. Allen Miller, Bryony C. Bonning, Amy L. Toth

Ecology, Evolution and Organismal Biology Publications

Honeybee population declines have been linked to multiple stressors, including reduced diet diversity and increased exposure to understudied viral pathogens. Despite interest in these factors, few experimental studies have explored the interaction between diet diversity and viral infection in honeybees. Here, we used a mixture of laboratory cage and small semi-field nucleus hive experiments to determine how these factors interact. In laboratory experiments, we found that high-quality diets (polyfloral pollen and high-quality single-source pollen) have the potential to reduce mortality in the face of infection with Israeli acute paralysis virus (IAPV). There was a significant interaction between diet and virus ...


Hexapod Herald - Vol. 31, No. 1, February 2019, 2019 University of Nebraska - Lincoln

Hexapod Herald - Vol. 31, No. 1, February 2019

Hexapod Herald & Other Entomology Department Newsletters

Welcome -- Congratulations -- Note from John Ruberson -- Grants -- Faculty News -- Student News -- Publications -- Blast from the Past -- Brewer Appreciation Reception -- Intro Beekeeping Class-Year 1 -- Condolences -- Retirement Reception for Kalisch and Roselle


Genome-Wide Characterization, Molecular Evolution And Mexpression Profiling Of The Metacaspases In Potato (Solanum Tuberosum L.), Nehal Dubey, Maitri Trivedi, Suresh Varsani, Vishal Vyas, Manisha Farsodia, Sunil Kumar Singh 2019 The M.S. University of Baroda

Genome-Wide Characterization, Molecular Evolution And Mexpression Profiling Of The Metacaspases In Potato (Solanum Tuberosum L.), Nehal Dubey, Maitri Trivedi, Suresh Varsani, Vishal Vyas, Manisha Farsodia, Sunil Kumar Singh

Faculty Publications: Department of Entomology

Metacaspases are distant relatives of animal caspases found in plants, protozoa and fungi. Some recent studies have demonstrated that metacaspases are involved in regulating the developmental and environmentally induced programmed cell death in plants. In this study, we identified metacaspase gene family in potato (Solanum tuberosum L.) and analyzed their expression pattern in various developmental tissues and stress responses of plants. There were eight metacaspase genes identified in the Peptidase (Cysteine protease) C14 family and based upon sequence alignment and phylogenetic analysis, a systematic nomenclature of potato metacaspases (SotubMCs) has been proposed. Three of the eight candidate genes showing homology ...


South Dakota Aquatic Invertebrate Collection And Database, Ashlee Nilson, Nels H. Troelstrup Jr. 2019 South Dakota State University

South Dakota Aquatic Invertebrate Collection And Database, Ashlee Nilson, Nels H. Troelstrup Jr.

Oak Lake Field Station 30th Anniversary Retreat Presentations

No abstract provided.


Native Grass And Legume Biology And Establishment, Arvid A. Boe, P. J. Johnson 2019 South Dakota State University

Native Grass And Legume Biology And Establishment, Arvid A. Boe, P. J. Johnson

Oak Lake Field Station 30th Anniversary Retreat Presentations

No abstract provided.


Differential Spatial Gradients Of Wheat Streak Mosaic Virus Into Winter Wheat From A Central Mite-Virus Source, Abby R. Stilwell, Donald Rundquist, David B. Marx, Gary L. Hein 2019 USDA APHIS

Differential Spatial Gradients Of Wheat Streak Mosaic Virus Into Winter Wheat From A Central Mite-Virus Source, Abby R. Stilwell, Donald Rundquist, David B. Marx, Gary L. Hein

Faculty Publications: Department of Entomology

The wheat curl mite (WCM), Aceria tosichella Keifer, transmits three potentially devastating viruses to winter wheat. An increased understanding of mite movement and subsequent virus spread through the landscape is necessary to estimate the risk of epidemics by the virus in winter wheat. Owing to the small size of WCMs, their dispersal via wind is hard to monitor; however, the viruses they transmit produce symptoms that can be detected with remote sensing. The objective of this study was to characterize the spatial dispersal of the virus from a central mite-virus source. Virus infection gradients were measured spatially by using aerial ...


Native Solitary Bees Provide Economically Significant Pollination Services To Confection Sunflowers (Helianthus Annuus L.) (Asterales: Asteraceae) Grown Across The Northern Great Plains, Rachel E. Mallinger, Jeff Bradshaw, Adam J. Varenhorst, Jarrad R. Prasifka 2019 University of Florida

Native Solitary Bees Provide Economically Significant Pollination Services To Confection Sunflowers (Helianthus Annuus L.) (Asterales: Asteraceae) Grown Across The Northern Great Plains, Rachel E. Mallinger, Jeff Bradshaw, Adam J. Varenhorst, Jarrad R. Prasifka

Faculty Publications: Department of Entomology

The benefits of insect pollination to crop yields depend on genetic and environmental factors including plant selffertility, pollinator visitation rates, and pollinator efficacy. While many crops benefit from insect pollination, such variation in pollinator benefits across both plant cultivars and growing regions is not well documented. In this study, across three states in the northern Great Plains, United States, from 2016 to 2017, we evaluated the pollinatormediated yield increases for 10 varieties of confection sunflowers, Helianthus annuus L. (Asterales: Asteraceae), a plant that is naturally pollinator-dependent but was bred for self-fertility. We additionally measured pollinator visitation rates and compared per-visit ...


Advances In Molecular Research On Bed Bugs (Hemiptera: Cimicidae), Sanjay Basnet, Shripat T. Kamble 2019 University of Nebraska - Lincoln

Advances In Molecular Research On Bed Bugs (Hemiptera: Cimicidae), Sanjay Basnet, Shripat T. Kamble

Faculty Publications: Department of Entomology

With the resurgence and increase in infestations of the common bed bug, Cimex lectularius L. (Hemiptera: Cimicidae), across the world, there has been renewed interest in molecular research on this pest. In this paper, we present current information on the biology, medical importance, management practices, behavior and physiology, and molecular research conducted on bed bugs. The majority of molecular studies are focused towards understanding the molecular mechanism of insecticide resistance. Bed bugs are hematophagous insects with no prior record of vectoring any disease organisms. An improved understanding of how bed bugs lack vector competency may provide information to prevent disease ...


Rnai Targeting Of Rootworm Troponin I Transcripts Confers Root Protection In Maize, Elane Fishilevich, Andrew J. Bowling, Meghan L. F. Frey, Po-Hao Wang, Wendy Lo, Murugesan Rangasamy, Sarah E. Worden, Heather E. Pence, Premchand Gandra, Shannon L. Whitlock, Greg Schulenberg, Eileen Knorr, Linda Tenbusch, Jamie R. Lutz, Stephen Novak, Ronda L. Hamm, Karl D. Schnelle, Andreas Vilcinskas, Kenneth E. Narva 2019 University of Nebraska-Lincoln

Rnai Targeting Of Rootworm Troponin I Transcripts Confers Root Protection In Maize, Elane Fishilevich, Andrew J. Bowling, Meghan L. F. Frey, Po-Hao Wang, Wendy Lo, Murugesan Rangasamy, Sarah E. Worden, Heather E. Pence, Premchand Gandra, Shannon L. Whitlock, Greg Schulenberg, Eileen Knorr, Linda Tenbusch, Jamie R. Lutz, Stephen Novak, Ronda L. Hamm, Karl D. Schnelle, Andreas Vilcinskas, Kenneth E. Narva

Faculty Publications: Department of Entomology

Western corn rootworm, Diabrotica virgifera virgifera, is the major agronomically important pest of maize in the US Corn Belt. To augment the repertoire of the available dsRNA-based traits that control rootworm, we explored a potentially haplolethal gene target, wings up A (wupA), which encodes Troponin I. Troponin I, a component of the Troponin-Tropomyosin complex, is an inhibitory protein involved in muscle contraction. In situ hybridization showed that feeding on wupA-targeted dsRNAs caused systemic transcript knockdown in D. v. virgifera larvae. The knockdown of wupA transcript, and by extension Troponin I protein, led to deterioration of the striated banding pattern ...


A New Species And Synonymy In North American Phymatodes (Phymatodes) Mulsant, 1839 (Coleoptera: Cerambycidae: Cerambycinae: Callidiini), James E. Wappes, Antonio Santos-Silva 2019 American Coleoptera Museum, San Antonio, TX

A New Species And Synonymy In North American Phymatodes (Phymatodes) Mulsant, 1839 (Coleoptera: Cerambycidae: Cerambycinae: Callidiini), James E. Wappes, Antonio Santos-Silva

Insecta Mundi

Phymatodes (Phymatodes) huetheri Wappes and Santos-Silva (Coleoptera: Cerambycidae: Cerambycinae: Callidiini) from upstate New York, USA and Canada is described and illustrated. Phymatodes (Phymatodes) rainieri Van Dyke, 1937 is placed in synonymy with Phymatodes (Phymatodes) fulgidus Hopping, 1928.


Description Of A New Species Of Melanesiandra Santos-Silva Et Al. (Coleoptera: Cerambycidae: Parandrinae) Found In Vanuatu, Shunsuke Kakinuma 2019 Tokyo University of Agriculture and Technology

Description Of A New Species Of Melanesiandra Santos-Silva Et Al. (Coleoptera: Cerambycidae: Parandrinae) Found In Vanuatu, Shunsuke Kakinuma

Insecta Mundi

Melanesiandra anatomensis Kakinuma, new species (Coleoptera: Cerambycidae: Parandrinae), found in Aneityum Island (Vanuatu), is described and illustrated. An updated key is presented to separate the six known species of the genus.


Descripción De Una Especie Nueva Y Dos Nuevos Registros De Paraleurolobus Sampson Y Drews (Hemiptera: Aleyrodidae) De Guatemala, José Francisco García Ochaeta, Oscar Ángel Sánchez-Flores 2019 Ministerio de Agricultura Ganadería y Alimentación, Petén, Guatemala

Descripción De Una Especie Nueva Y Dos Nuevos Registros De Paraleurolobus Sampson Y Drews (Hemiptera: Aleyrodidae) De Guatemala, José Francisco García Ochaeta, Oscar Ángel Sánchez-Flores

Insecta Mundi

Se describe Paraleurolobus evansi García-Ochaeta y Sánchez-Flores sp. nov. (Hemiptera: Aleyrodidae) de Guatemala. Se comentan los caracteres diferenciales de esta especie con sus congéneres. Se incluye una clave para determinar las especies de Paraleurolobus Sampson y Drews, y se reportan por primera vez para Guatemala a P. chamaedoreae Russell y P. imbricatus Sampson y Drews.

Paraleurolobus evansi García-Ochaeta y Sánchez-Flores sp. nov. (Hemiptera: Aleyrodidae) is described from Guatemala. Differential characters between this and related species are discussed. A key is included to identify Paraleurolobus Sampson y Drews species, and P. chamaedoreae Russell and P. imbricatus Sampson y Drews are reported ...


Three New Madagascan Species Of Ochodaeus Dejean (Coleoptera: Scarabaeoidea: Ochodaeidae), M. J. Paulsen 2019 University of Nebraska State Museum

Three New Madagascan Species Of Ochodaeus Dejean (Coleoptera: Scarabaeoidea: Ochodaeidae), M. J. Paulsen

Insecta Mundi

Adults of three known species of Ochodaeus Dejean (Coleoptera: Scarabaeoidea: Ochodaeidae) in Madagascar have a strongly granulate pronotum. Three new species are described herein that have a smooth or weakly granulate pronotum: Ochodaeus modopunctatus, O. polypollicatus, and O. meandrus. A key to the species of Ochodaeus from Madagascar is provided.


Opda Regulates Maize Defense Against Aphids, Suresh Varsani, Sajjan Grover, Shaoqun Zhou, Kyle G. Koch, Pei-Cheng Huang, Michael V. Kolomiets, W. Paul Williams, Tiffany Heng-Moss, Gautam Sarath, Dawn S. Luthe, Georg Jander, Joe Louis 2019 University of Nebraska-Lincoln

Opda Regulates Maize Defense Against Aphids, Suresh Varsani, Sajjan Grover, Shaoqun Zhou, Kyle G. Koch, Pei-Cheng Huang, Michael V. Kolomiets, W. Paul Williams, Tiffany Heng-Moss, Gautam Sarath, Dawn S. Luthe, Georg Jander, Joe Louis

Faculty Publications: Department of Entomology

Copyright © 2019 American Society of Plant Biologists.

DOI:10.1104/pp.18.01472


Cold Tolerance, Temperature Mediated Discontinuous Gas Exchange, And Emergence Of The Blue Orchard Mason Bee (Osmia Lignaria), Logan Kral 2019 Central Washington University

Cold Tolerance, Temperature Mediated Discontinuous Gas Exchange, And Emergence Of The Blue Orchard Mason Bee (Osmia Lignaria), Logan Kral

All Master's Theses

The relationship between low temperatures, emergence and supercooling point of Osmia lignaria were the subject of this study. One hundred sixty-eight bees were subjected to 5 pre-wintering temperature treatments (two constant temperature controls - 22oC, 14oC, one of which with and one without a 12h photoperiod, and three 12h:12h thermoperiod treatments – 14:10oC, 14:5oC, and 14:0oC) and were then evaluated in terms of emergence time and post-emergence vigor. An additional 70 bees were tested for metabolic rate and discontinuous gas exchange in response to test temperature conditions. An ...


Effects Of Diet Quality And Temperature On Stable Fly (Diptera: Muscidae) Development, Melina Florez-Cuadros, Dennis Berkebile, Gary Brewer 2019 University of Nebraska - Lincoln

Effects Of Diet Quality And Temperature On Stable Fly (Diptera: Muscidae) Development, Melina Florez-Cuadros, Dennis Berkebile, Gary Brewer

Faculty Publications: Department of Entomology

The effects of diet quality and temperature on the development time and size of stable flies, Stomoxys calcitrans (L.), was evaluated. Both development time and size varied relative to diet quality and temperature, and their effects were additive. Diet quality and temperature made similar contributions to the variance in size whereas temperature was responsible for >97% of the variance in development time. Regression analysis predicted the shortest development time, egg to adult, to be 12.7 days at 32 C and 70% nutrients. Egg to adult development varied curvilinearly relative to diet quality and temperature on the degree day 10 ...


Characterization Of Lipids And The Protein Co-Products From Various Food Sources Using A One-Step Organic Solvent Extraction Process, Alleda Rose 2019 West Virginia University

Characterization Of Lipids And The Protein Co-Products From Various Food Sources Using A One-Step Organic Solvent Extraction Process, Alleda Rose

Graduate Theses, Dissertations, and Problem Reports

Traditional lipid extraction methods are labor intensive and time-consuming so interest in one-step methods using various organic solvents are gaining in popularity. Extraction efficiency depends heavily on the solvents used yet comparisons between different solvents are lacking. Defatting techniques are commonly used to concentrate crude protein and may be useful for enhancing protein content in food sources. The objective of this research was to 1) determine the lipid extraction efficiencies of hexane (H), 3:2 hexane-isopropanol (HI), chloroform (C), 2:1 chloroform-methanol (CM), and methyl-tert-butyl-ether (MTBE) using a one-step organic solvent lipid extraction method on whole insect, egg yolk, and ...


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