Dairy Science Commons^™

Distillers' Grains With Solubles For Feedlot Cattle: Finishing Performance, Lipid Metabolism, And Ethanol Greenhouse Gas Balance, Virgil R. Bremer

Department of Animal Science: Dissertations, Theses, and Student Research

Previous University of Nebraska feedlot research trials have characterized the feed value of wet distillers grains plus solubles (WDGS). These trials were summarized with meta-analysis methodology and indicated the feed value of WDGS interacts with corn processing type, cattle age (calf-fed or yearling), and inclusion level. Two steer finishing studies and a metabolism study were conducted to understand the impact of different lipid sources in WDGS on WDGS feed value. A biphasic lipid extraction procedure was developed to analyze feed samples from these trials that was more effective than Goldfisch ether extraction at lipid analysis of byproduct feds. These trials …

A Novel Method For Evaluating The Release Of Fermentable Sugars From Cellulosic Biomass, Rafael Jiménez-Flores, Gina Fake, Jennifer Carroll, Elizabeth Hood, John Howard

Dairy Science

Efficient hydrolysis of cellulosic biomass into free sugars for the production of bioethanol would allow utilization of an abundant and renewable natural resource. However, complex microcrystalline cellulose in biomass resists digestion. A preferred method to deconstruct cellulose is using enzyme cocktails, but the presence of multiple enzymes with synergistic outcomes for this process complicates direct biochemical analysis as a reliable indicator of deconstruction. Methods to detect release of free sugars rather than direct measurement of enzymatic activity are available but are time-consuming, and do not reflect the production of metabolic inhibitors of microbial growth which may interfere with the subsequent …

Phosphorous In Dairy Cattle Diets, Rhonda Miller, Allen Young, Jennifer Major, Lydia Trinca

Agriculture

No abstract provided.

Report On Phosphorus And Potassium Experiments Conducted On Partner Farms Of The Greener Pastures Project, Mike Bolland, Ian Guthridge, Graham Blincow, Peter Needs

Bulletins 4000 -

Dairy production in south-western Australia occurs predominantly on sandy soils in the greater than 800 mm annual average rainfall areas of the region. The climate is Mediterranean-type, typically with cool, wet May-October growing seasons and hot, dry November-April. Most dairy pastures are rain-fed and comprise the annual species Subterranean Clover (clover, Trifolium subterraneum L.), Annual Ryegrass (Lolium rigidum Gaud.) and Italian Ryegrass (L. multiflorum Lam.).

Paddock grown pasture is the cheapest source of feed for dairy production in the region (Dilley and Howes 1998). To remain profitable in the face of diminishing returns, dairy farmers have modified their grazing management …

Phosphorous In Dairy Cattle Diets, Rhonda Miller, Allen Young, Jennifer Major, Lydia Trinca

All Current Publications

No abstract provided.

The Effect Of Claw Horn Disruption Lesions And Body Condition Score At Dry-Off On Survivability, Reproductive Performance, And Milk Production In The Subsequent Lactation, V. S. Machado, L. S. Caixeta, J. A. A. Mcart, R. C. Bicalho

Farm Animal Welfare Collection

The objective of this study was to evaluate the effects of claw horn disruption lesions (CHDL; sole ulcers and white line disease) and body condition score (BCS) at dry-off on survivability, milk production, and reproductive performance during the subsequent lactation. An observational prospective cohort study was conducted on a large commercial dairy in Cayuga County, New York, from September 2008 until January 2009. A total of 573 cows enrolled at dry-off were scored for body condition and hoof trimmed; digits were visually inspected for the presence of CHDL. The BCS data were recategorized into a 3-level variable BCS group (BCSG), …

Delivering Health And Wellness, Phillip S. Tong

Dairy Science

No abstract provided.

The Impact Of Antioxidant Addition On Flavor Of Cheddar And Mozzarella Whey And Cheddar Whey Protein Concentrate, I. W. Liaw, H. Eshpari, P. S. Tong, M. A. Drake

Dairy Science

Lipid oxidation products are primary contributors to whey ingredient off-flavors. The objectives of this study were to evaluate the impact of antioxidant addition in prevention of flavor deterioration of fluid whey and spray-dried whey protein. Cheddar and Mozzarella cheeses were manufactured in triplicate. Fresh whey was collected, pasteurized, and defatted by centrifugal separation. Subsequently, 0.05% (w/w) ascorbic acid or 0.5% (w/w) whey protein hydrolysate (WPH) were added to the pasteurized whey. A control with no antioxidant addition was also evaluated. Wheys were stored at 3 °C and evaluated after 0, 2, 4, 6, and 8 d. In a subsequent experiment, …

Effects On Lactation Performance When Slick Hair Gene Is Simulated In Dairy Cattle In The Tropics, R. M. Mejía, J. A. Ortuño, G. J. Lascano, M. Vélez

Dairy Science

No abstract provided.

Digestibility Of Fresh Sugarcane-Based Diets With Slow-Release Non Protein Nitrogen Addition For Limit-Fed Dairy Heifers In The Tropics, G. J. Lascano, M. Velez, J. M. Tricarico, A. J. Heinrichs

Dairy Science

No abstract provided.

Dietary Starch Level And Dose Response Of Saccharomyces Cerevisiae For Limit Fed-Dairy Heifers, G. J. Lascano, J. M. Tricarico, A. J. Heinrichs

Dairy Science

No abstract provided.

Nutrient Utilization Of Different Levels Of Dietary Fiber In Dairy Heifers Limit-Fed High And Low Concentrate Diets, G. J. Lascano, A. J. Heinrichs

Dairy Science

No abstract provided.

Dairy Farmer Attitudes And Empathy Toward Animals Are Associated With Animal Welfare Indicators, Camilla Kielland, Eystein Skjerve, Olav Østerås, Adroaldo José Zanella

Societal Attitudes Toward Animals Collection

Attitudes and empathy of farmers influence human–animal interaction, thereby affecting their behavior toward animals. The goal was to investigate how measures of attitude and empathy toward animals were associated with animal welfare indicators such as milk yield, mastitis incidence, fertility index, and the prevalence of skin lesions on cows. To assess empathy toward animals, a photo-based pain assessment instrument was developed depicting various conditions that could be associated with some degree of pain in cattle and included questions aimed at assessing attitudes toward animals. Photos of painful conditions are useful in eliciting measurable empathic responses to pain in humans. A …

Growth Hormone Alters Lipid Composition And Increases The Abundance Of Casein And Lactalbumin Mrna In The Mac-T Cell Line, Tasha L. Johnson, Brent A. S. Fujimoto, Rafael Jiménez-Flores, Daniel G. Peterson

Dairy Science

The MAC-T cell line has been used extensively to investigate bovine mammary epithelial cell function. A lactogenic phenotype is generally induced in this cell line by a combination of dexamethasone, insulin and prolactin and has typically been assessed by milk protein production. Few studies have focused on identifying other factors that may affect milk protein synthesis in the MAC-T cell line, and none have considered the lipid class distribution of MAC-T cells as a component of the lactogenic phenotype. Growth hormone (GH) has been shown to increase milk protein synthesis both in vivo and in mammary cell models, and has …

Lipid Rafts In The Bovine Milk Fat Globule Membrane Revealed By The Lateral Segregation Of Phospholipids And Heterogeneous Distribution Of Glycoproteins, Christelle Lopez, Marie-Noëlle Madec, Rafael Jiménez-Flores

Dairy Science

This study reveals the lateral organisation of the milk fat globule membrane (MFGM). Using confocal laser scanning microscopy (CLSM) and a lipid soluble molecule, an exogenous phospholipid and two lectins as fluorescent probes we located triacylglycerols in the core of fat globules and investigated the organisation of the polar lipids and glycoproteins of the MFGM, in situ in milk. Lipid rafts corresponding to the lateral segregation of sphingolipids in liquid-ordered phases surrounded by liquid-disordered domains composed by the glycerophospholipids were observed in the MFGM. These lipid rafts which correspond to rigid sphingolipid-rich domains have a circular shape at room temperature. …

Culturally Speaking: Beyond Yogurt, Phillip S. Tong

Dairy Science

No abstract provided.

Measures Of Weight Distribution Of Dairy Cows To Detect Lameness And The Presence Of Hoof Lesions, M. Pastell, L. Hänninen, A. M. De Passillé, J. Rushen

Farm Animal Welfare Collection

There is increasing interest in automated methods of detecting lame cows. Hoof lesion data and measures of weight distribution from 61 lactating cows were examined in this study. Lame cows were identified with different numerical rating scores (NRS) used as thresholds (NRS >3 and NRS ≥3.5) for lameness. The ratio of weight applied to a pair of legs (LWR) when the cow was standing was calculated using a special weigh scale, and the cows were gait scored using a 1 to 5 NRS. Hoof lesions were scored and the cows placed into 1 of 4 mutually exclusive categories of hoof …

Assessment Of Lameness Prevalence And Associated Risk Factors In Dairy Herds In England And Wales, Z. E. Barker, K. A. Leach, H. R. Whay, N. J. Bell, D. C. J. Main

Farm Animal Welfare Collection

Visits were made to 205 dairy farms in England and Wales between October 2006 and May 2007 by 1 or more of 4 researchers. At each visit, all milking cows were locomotion scored (lameness scored) using a 4-point scale (0 = sound locomotion, 1 = imperfect locomotion, 2 = lame, 3 = severely lame). The mean prevalence of lameness (scores 2 and 3) across the study farms was 36.8% (range = 0–79.2%). On each farm, the presence within the housing and grazing environments of commonly reported risks for increased lameness was recorded. Each farmer was interviewed to gauge the ability …

Culturally Speaking: The Year Of Sodium, Phillip S. Tong

Dairy Science

No abstract provided.

Dairy Animal Care: Quality Assurance

Nebraska Beef Quality Assurance Program

Dairy producers are encouraged to participate in the voluntary Dairy Animal Care and Quality Assurance (DACQA) Certification program. The purpose of this program is to enhance and demonstrate quality animal care practices, which assure food safety, quality and value as well as enhance consumer confidence in the milk and beef products that are harvested from cattle on America’s dairy farms.

Respiratory Disease In Young Dairy Calves., A. D. Garcia, R. Daly

Dairy Science Publication Database

Respiratory disease is the second leading cause of death losses (scours is the first) in un-weaned heifer calves. Respiratory problems have increased by 34 percent in the last twenty years resulting in nearly 21 percent of all newborn calf losses (NAHMS. 2007). Heifers that survive continue to perform poorly as adult cows. In order to prevent this costly problem it is important to address both determinant and predisposing causes.

Sdsu Dairy Digest 2010, South Dakota State University Dairy Club

Dairy Digest

No abstract provided.

The Jackrabbit Dairy Review, Vikram Mistry

The Jackrabbit Dairy & Food Review

No abstract provided.

Enfermedad Respiratoria En Los Terneros Lecheros, Como Prevenirla, A. D. Garcia, A. R. Daly

Dairy Science Publication Database

La enfermedad respiratoria es la segunda causa de muerte (diarrea es la primera) en las terneras lecheras no destetadas. Los problemas respiratorios han aumentado en un 34 por ciento en los últimos 20 años, causando cerca de 21 por ciento de la mortandad de los terneros (NAHMS 2007). Las terneras que sobreviven continúan con un desempeño pobre al convertirse en vacas adultas. Para prevenir este problema, es importante tener en cuenta tanto causas predisponentes como determinantes.

Investigating The Protective Properties Of Milk Phospholipids Against Ultraviolet Light Exposure In A Skin Equivalent Model, Ashley Russell, Andrea Laubscher, Rafael Jiménez-Flores, Lily H. Laiho

Dairy Science

Current research on bioactive molecules in milk has documented health advantages of bovine milk and its components. Milk Phospholipids, selected for this study, represent molecules with great potential benefit in human health and nutrition. In this study we used confocal reflectance and multiphoton microscopy to monitor changes in skin morphology upon skin exposure to ultraviolet light and evaluate the potential of milk phospholipids in preventing photodamage to skin equivalent models. The results suggest that milk phospholipids act upon skin cells in a protective manner against the effect of ultraviolet (UV) radiation. Similar results were obtained from MTT tissue viability assay …

Development Of A New Tacaribearenavirus Infection Model And Its Use To Explore Antiviral Activity Of A Novelaristeromycin Analog, Brian B. Gowen, M. H. Wong, D. Larson, W. Ye, K. H. Jung, E. J. Sefing, Ramona T. Skirpstunas, Donald F. Smee, John D. Morrey, S. W. Schneller

Animal, Dairy, and Veterinary Science Faculty Publications

Background

A growing number of arenaviruses can cause a devastating viral hemorrhagic fever (VHF) syndrome. They pose a public health threat as emerging viruses and because of their potential use as bioterror agents. All of the highly pathogenic New World arenaviruses (NWA) phylogenetically segregate into clade B and require maximum biosafety containment facilities for their study. Tacaribe virus (TCRV) is a nonpathogenic member of clade B that is closely related to the VHF arenaviruses at the amino acid level. Despite this relatedness, TCRV lacks the ability to antagonize the host interferon (IFN) response, which likely contributes to its inability to …

Influence Of Low Homogenization Pressures On Acid Tolerance, Bile Tolerance, Protease Activity, And Growth Characteristics Of Lactobacillus Delbrueckii Ssp Bulgaricus Lb-12, Streptococcus Salivarius Ssp Thermophilus St-M5, And Lactobacillus Acidophilus La-K, Tanuja Muramalla

LSU Master's Theses

Lactobacillus delbrueckii ssp bulgaricus LB-12, Streptococcus salivarius ssp thermophilus ST-M5 and Lactobacillus acidophilus LA-K are dairy cultures widely used in the fermentation of dairy products. Homogenization is an essential process in dairy industry for making several products. High homogenization pressures (>50 MPa) are used to create stable emulsion, inactivate the pathogens and increase the protease activity of dairy cultures. Sparse literature is available on the influence of low homogenization pressures, and their effect on beneficial characteristics of dairy cultures is not well understood. The objective of this study was to determine the effect of low homogenization pressures on acid …

Dopaminergic Input To The Equine Pituitary: Seasonal And Estradiol Effects, Sarah Case Clavier

LSU Master's Theses

Two experiments were conducted to study the effects of season and estradiol administration on dopaminergic input to the equine adenohypophysis. Experiment 1 began in the spring with 8 mares and 8 stallions and was repeated again in the summer, fall, and winter. Horses were given sulpiride injections of eight incremental doses (0.25 to 32 µg/kg BW). Within each group of 8 horses, half received the sulpiride in an increasing manner, the other half in a decreasing manner. Prolactin concentrations peaked in the first 15 to 30 min in all horses in all seasons. Prolactin areas under the curve increased (P < 0.001) with increasing doses of sulpiride, and were highest (P < 0.05) in March for stallions, but in June for mares. The calculated half-maximum values, which should be proportional to the dopaminergic input to the pituitary, were lowest (P < 0.05) in June and greatest in September. The variation in half-maximum values with season indicated a change in dopaminergic input to the pituitary, with lowest input occurring in June in both sexes. Experiment 2 was designed to determine if the degree of dopaminergic input to the adenohypophysis is altered by estradiol administration. Twelve geldings were used. On day 0, geldings received an i.m. injection of either estradiol cypionate (ECP; n = 6) or vegetable oil (n = 6) at one of two dose volumes (2 vs. 10 mL). On day 6, all geldings received an injection of sulpiride at 0.082 µg/kg BW in saline. Sulpiride injections were repeated every other day with the dose increasing each day, from 0.164 to 100 µg/kg BW. Estradiol pretreatment increased (P < 0.05) the prolactin response to sulpiride at the 2.56 µg/kg dose and higher. However, the half-maximum values for ECP-treated and control geldings did not differ, indicating that the amount of sulpiride needed to counterbalance the amount of dopamine reaching the pituitary was unaltered by estradiol treatment. It was concluded that estradiol likely stimulates prolactin production and secretion after sulpiride directly at the lactotrope level, rather than by decreasing hypothalamic dopamine input to the lactotropes.

Induction Of Ip-10 By Sars-Cov Infection Of Calu-3cells And Balb/C Mice, Y. Kumaki, C. W. Day, K. W. Bailey, M. H. Wong, M. K. Wandersee, R. Madsen, J. S. Madsen, N. M. Nelson, J. D. Hoopes, J. D. Woolcott, Z. T. Mclean, L. M. Blatt, A. M. Salazar, Dale L. Barnard

Animal, Dairy, and Veterinary Science Faculty Publications

Destruction of the architectural and subsequently the functional integrity of the lung following pulmonary viral infections is attributable to both the extent of pathogen replication and to the host-generated inflammation associated with the recruitment of immune responses. The presence of antigenically disparate pulmonary viruses and the emergence of novel viruses assures the recurrence of lung damage with infection and resolution of each primary viral infection. Thus, there is a need to develop safe broad spectrum immunoprophylactic strategies capable of enhancing protective immune responses in the lung but which limits immune-mediated lung damage. The immunoprophylactic strategy described here utilizes a protein …

Culturally Speaking: Ethnic Dairy Foods, Phillip S. Tong

Dairy Science

No abstract provided.