Life Sciences Commons^™

The Reveal: A Technical Study And Conservation Treatment Of An Overpaint Portrait, Camille Ferrer

Art Conservation Master's Projects

A severely damaged 19th-century oil painting depicting a portrait of a woman was treated at Patricia H. and E. Garman Art Conservation Department. A typed letter provided by the owner mentioned that it has been previously restored yet returned with unsatisfactory results. After further examination, the painting appeared to have been previously treated multiple times by different people. There was overpaint distinctly present on the face and later discovered to be present overall. The full state of condition of the painting was initially unknown due to the sum of the surface being overpainted. However, there were evidence of paint loss …

Ncaa Di Student-Athletes’ Understanding Of And Attitudes Toward Mental Performance Services, Allison Rudisill

Honors Theses

Mental performance services are designed to assist student-athletes with psychological challenges affiliated with performance (McHenry et al., 2022). Currently, only 65 NCAA DI athletic institutions employ a psychological provider, with only 23.2% of those being mental performance consultants (MPCs; Jones et al., 2022). As student athletes are aware of the mental demand of sport (Bemiller & Wrisberg, 2011) and the NCAA begins to bring awareness to the importance of the mental well-being of the athlete (NCAA Transformational Committee, 2023), it is important to understand student athletes’ attitudes toward these services. Therefore, the purpose of this study was to learn about …

Optimization Of Tyrosine Hydroxylase Antibody For Immunohistochemistry Fluorescence Detection In Zebrafish (Danio Rerio), Madison Thurber

Theses/Capstones/Creative Projects

Dopamine is an important neurotransmitter produced through the catecholamine synthesis pathway that affects brain activity. Unregulated dopamine levels can lead to various diseases such as Parkinson’s Disease or attention deficit hyperactivity disorder (ADHD). Optimization of an immunohistochemistry protocol will allow for the quantification of tyrosine hydroxylase antibody, which indirectly allows for dopamine quantification in dopaminergic regions within the brain. However, the antibody concentration to give the optimal signal-to-noise ratio in IHC varies across studies. Through this experiment, I determined the concentration of tyrosine hydroxylase (TyrH) antibody for immunohistochemistry that gave the best signal-to-background noise ratio within several known dopaminergic regions …

The Future Of The Organization Of The Petroleum Exporting Countries, Mikayla Gross

Honors Theses

The Organization of the Petroleum Exporting Countries (OPEC) is a multinational and intergovernmental organization that dictates the petroleum exportation policies of 13 countries: Algeria, Angola, Equatorial Guinea, Gabon, Iran, Iraq, Kuwait, Libya, Nigeria, the Republic of the Congo, Saudi Arabia, the United Arab Emirates and Venezuela (About Us, 2023). The mission of OPEC is to ensure a stable supply of petroleum through its shared policies for its Member Countries and consumers (Our Mission, 2023). Iran, Iraq, Kuwait, Saudi Arabia, and Venezuela founded OPEC in 1960 under the foundations and principles that dictated the international world order. Those foundations and principles …

Machine Learning-Based Soybean Yield Prediction And Optimizing Lidar-Mounted Uav Efficiency, Leticia Santos

LSU Master's Theses

The first chapter of this thesis explores the predictive capabilities of random forests algorithm on datasets obtained from field plot experiments on crop management systems in soybean. Furthermore, the chapter presents a complementary analysis of model performance according to dataset sizes and two techniques on how to impute and deal with missing data. Random forests are being compared with standard statistical techniques such as linear regression on a well-structured, information-rich agronomic experiment. The key findings of this chapter includes the best hyperparameters adjustment and the identification of the dataset threshold for optimal algorithms performance. The second chapter has a research …

Estrogen Receptor (Er) Alpha Regulatory Mechanisms And Therapeutic Strategies In Er+ Breast Cancer, Bianca A. Romo

Dartmouth College Ph.D Dissertations

Breast cancer is among the most frequently diagnosed cancers in the U.S. and is one of the leading causes of cancer-related mortalities, second to lung cancer. Estrogen receptor alpha-positive (ER+) breast cancer accounts for 2/3 of diagnosed cases. Patients diagnosed with this subtype of breast cancer typically undergo endocrine therapy that aims to mitigate the growth-promoting effects of estrogen/ER. While therapies are effective, 1/3 of patients will experience recurrence. To begin addressing this drug-resistant patient population, we investigated potential drug targets involved in response to treatment.

Coregulators have been implicated in the regulation of ER transcriptional activity and subsequently affecting …

Target Selection And Enhancement During Attentional Tracking, Marvin R. Maechler

Dartmouth College Ph.D Dissertations

At any waking moment, we are bombarded with more sensory information than we can fully process. Attention is necessary to deal with the dynamic world we live in. One fundamental function of vision and attention is to keep track of moving objects, but what are the targets of attention during tracking?

One of the first theories of attentional tracking predicted that targets would be selected at early processing stages. By employing the double-drift illusion, which dissociates physical and perceived positions of moving objects, we investigated which of these positions is selected for tracking. Contrary to earlier theories and in line …

Assessing Lipid Composition Of Cell Membrane In Escherichia Coli Under Aerobic And Anaerobic Conditions, Isabelle Johnson

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Escherichia coli is a highly studied model organism that is tightly tied to the mammalian gastrointestinal system. This microorganism has the capability to be a beneficial gut microbe or a life-threatening pathogen. In this study, the lipid membrane of Escherichia coli was investigated using Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) to observe the change in its composition in aerobic and anaerobic conditions. Evidence of desaturation was discovered in the spectra, though more investigation is needed to understand the metabolic processes and drives that result in this change. Elucidation of these pathways in the future could result in …

Tropical Fish Study In Tahiti, French Polynesia, Miranda Brainard, Caitlyn Swango, Paityn Houglan, Richard Londraville

Williams Honors College, Honors Research Projects

In May of 2023, I embarked on an exciting research journey to Moorea, French Polynesia, alongside fellow students and faculty members from the University of Akron and Syracuse University. This expedition was part of the university-sponsored Tropical Vertebrate Biology course, where we delved into the exploration of various tropical species inhabiting the island, including sea urchins, geckos, and my primary focus, the blackspotted rockskipper.

My research team, composed of my co-authors and me, was particularly intrigued by the unique refuge-seeking behavior displayed by blackspotted rockskippers. These amphibious fish are renowned for their remarkable ability to inhabit tide pools and rocky …

Zebrafish Electroretinogram Responses, Brooke Campbell

Williams Honors College, Honors Research Projects

The goal of this project is to be able to streamline a protocol for conducting ERGs on zebrafish from mice ERG protocol already present in Dr. Renna’s lab. The objective is to create a protocol specifically for zebrafish and to eliminate any issues that occur when transiting from a mice ERG protocol to a zebrafish ERG protocol and to obtain a light response from zebrafish retinae in differing light intensities. Dr. Renna’s lab has designed an Ex Vivo ERG system with less electrical interference creating defined electrical responses from mouse retina. The setup allows for continual perfusion of the retinal …

From Grain To Malt: Tracking Changes Of Ultra-Low-Gluten Barley Storage Proteins After Malting, Mahya Bahmani, Angéla Juhász, Utpal Bos, Mitchell G. Nye-Wood, Malcolm Blundell, Crispin A. Howitt, Michelle L. Colgrave

Research outputs 2022 to 2026

Barley (Hordeum vulgare L.) is a major cereal crop produced globally. Hordeins, the major storage proteins in barley, can trigger immune responses leading to celiac disease or symptoms associated with food allergy. Here, proteomics approaches were employed to investigate the proteome level changes of grain and malt from the malting barley cultivar, Sloop, and single-, double- and triple hordein-reduced lines. The triple hordein-reduced line is an ultra-low gluten barley cultivar, Kebari®. Using discovery proteomics, 2,688 and 3,034 proteins in the barley and malt samples were detected respectively. Through the application of targeted proteomics, a significant reduction in the quantity …

Synthesis And Biophysical Analysis Of Modified Cell-Penetrating Peptides, Joel Mitchell

Theses and Dissertations (Comprehensive)

Cell-penetrating peptides (CPPs) are a family of peptides that have the ability to penetrate biological membranes. They were discovered in the late 1980s and have been the topic of many studies. Much of the interest in CPPs has been due to their ability to translocate biological membranes, and the possibility that they could offer a novel drug delivery method by conjugation to biologically active molecules. Linear CPPs can be modified to form cyclic structures. This change in structure has been observed to enhance the stability and penetrative ability of the CPPs which have been studied. The current thesis focuses on …

Variation In Mainland Northwest Territories Late-Winter Muskox (Ovibos Moschatus) Density Estimations And Habitat Associations Above And Below Treeline., Charlotte Rentmeister

Theses and Dissertations (Comprehensive)

The Arctic and Sub-Arctic ecosystems are seeing accelerated changes in temperature, landcover, and consequently species abundance and distributions. Reliable distributions, and associated population density estimates, are essential for effective conservation and management efforts. Growing concerns from northern communities regarding the relationship between muskox and declining caribou populations strengthens the need for updated information on muskox populations within mainland Northwest Territories (NWT). The first objective for my research was to quantify and map updated winter estimates of abundance, density, and distribution of muskoxen within three recent survey regions located in mainland NWT, using a multiple covariate distance sampling method (MCDS), paired …

Mitochondrial Metabolism In Blood More Reliably Predicts Whole-Animal Energy Needs Compared To Other Tissues, Stefania Casagrande, Maciej Dzialo, Lisa Trost, Kasja Malkoc, Edyta T. Sadowska, Michaela Hau, Barbara J. Pierce, Scott R. Mcwilliams, Ulf Bauchinger

Biology Faculty Publications

Understanding energy metabolism in free-ranging animals is crucial for ecological studies. In birds, red blood cells (RBCs) offer a minimally invasive method to estimate metabolic rate (MR). In this study with European starlings Sturnus vulgaris, we examined how RBC oxygen consumption relates to oxygen use in key tissues (brain, liver, heart, and pectoral muscle) and versus the whole-organism measured at basal levels. The pectoral muscle accounted for 34-42% of organismal MR, while the heart and liver, despite their high mass-specific metabolic rate, each contributed 2.5-3.0% to organismal MR. Despite its low contribution to organismal MR (0.03-0.04%), RBC MR best predicted …

Single Night Surveys Of Moth Communities Can Serve As Ultra-Rapid Biodiversity Assessments, Daniel P. Duran, Matthew Timar, Blaine Rothauser

School of Earth & Environment Faculty Scholarship

Biodiversity conservation decisions are typically based on limited data and resources. For this reason, there is great interest in surveying taxa that may allow for a rapid assessment of the biodiversity at a site. Numerous taxa have been proposed and utilized for rapid assessments that allow for such a survey in a matter of weeks or less. Herein, we test the idea that nocturnal moths have many of the characteristics that make them ideal for such surveys, such as relative ease of identification, strong ecological association with specific plant species and habitats, high alpha diversity, extended seasonal activity, and ease …

Nitrogen Fertilization And Harvest Management Improve Forage And Crude Protein Content In Crabgrass, B. C. Pedreira, I. T. M. D. Barros, J. I. Yasuoka, D. Helwig, J. K. Farney, G. F. Sassenrath

IGC Proceedings (2001-2023)

Crabgrass (Digitaria spp.) is an annual summer grass that can provide high-quality forage, but optimal management strategies are unclear. Our objective was to compare the yield and quality of crabgrass (Mojo and Quick-N-Big) under different nitrogen rates and harvest management. The experimental design was a randomized complete block with five treatments and three replications for each crabgrass variety, totaling fifteen experimental units for both Mojo and Quick-N-Big, in adjacent sites. Treatments were nitrogen rates (0, 100, and 200 lb N/acre) and harvest management (cut once or twice per year) for two growing seasons (2020 and 2021). Total forage accumulation …

Chemical Composition And Microbial Diversity Of The Silage Of Forage Peanut Harvested At Two Regrowth Ages And Treated With Microbial Inoculants, A. J. S. Macêdo, O. G. Pereira, V. P. Da Silva, A. J. Dos Anjos, W. Alves, H. R. O. Santos, K. G. Ribeiro

IGC Proceedings (2001-2023)

The objective of this study was to evaluate the chemical composition and microbial diversity of the silage of forage peanut harvested at 40 and 80 days of regrowth and treated with microbial inoculants. The inoculants evaluated were commercial inoculant (CI) Sil-All 4×4 (Lallemand®, Brazil); the AV14.17, Lactiplantibacillus pentosus strain (ISO) isolated from alfalfa silage; and control (CTRL), without inoculant. A total of 500 g of forage was ensiled in nylon-polyethylene bags (25.40 × 35.56 cm). After 60 days of fermentation, the bags were opened and silages were analyzed for dry matter (DM), mineral matter (MM), organic matter (OM), crude protein …

Evapotranspiration Of Rewetted And Drained Fen Soils With Grass – Long Term Lysimeter Studies, M. Pesch, J. Hoysagk, F. Eulenstein, A. Behrendt

IGC Proceedings (2001-2023)

In the context of reducing greenhouse gas (GHG) emissions to combat climate change, rewetting of peatlands is of particular importance. High groundwater levels covering organic matter in the soil prevent its oxidation to CO₂. The amount of water needed to allow the rewetting of peatlands can be determined in lysimeter studies.

The evapotranspiration in fens depends essentially on the groundwater table, the vegetation (transpirational active biomass) and the saturation deficit of the atmosphere. After rewetting, these factors can lead to an evapotranspiration of 1000 l m^-2 a^-1 and even 2000 l m^-2 a^-1 in …

Industrial Hemp Forage Potential, Swarup Podder, John H. Fike, B. Wilson

IGC Proceedings (2001-2023)

Hemp (Cannabis sativa L.) has been a valuable species for humans throughout history due to its adaptability and diverse uses. Farmers are interested in hemp as a forage and feedstuff due to its unique nutritional properties and fast summer production. Moreover, the presence of cannabinoids in leaf and bud tissues may provide pharmacological benefits to animal health, productivity, and product quality under different regulatory frameworks. This study examined two hemp cultivars, Grandi and Joey, solely as forage crops, and samples were taken at different growth stages in Blacksburg, VA, USA, in 2021. The highest biomass production of 1.41 tons per …

Differential Responses Of Yield And Shoot Traits Of Five Tropical Grasses To N And Distance To Trees In Silvopastoral Systems, L. S. Pontes, Emilio A. Laca

IGC Proceedings (2001-2023)

Light intensity and nitrogen (N) availability are important factors influencing the growth of C₄ forage species. Trade-offs may occur in the adaptive responses of species to shading and N inputs, and functional shoot traits can help to explain the consequences of these responses for species performance. Our objective was to gain understanding of the mechanisms between traits of five C₄ perennial grasses determining above-ground dry matter yield (DMY) when both resources, light and N, vary. Forage grasses were grown in six shading conditions (full sunlight vs. five positions between Eucalyptus dunnii rows) with two N levels (0 vs. …