Dairy Science Commons^™

Methane Production By A Packed-Bed Anaerobic Digester Fed Dairy Barn Flush Water, Sean Richard Thomson

Master's Theses

Packed-bed digesters are an alternative to covered lagoon digesters for methane production and anaerobic treatment of dilute wastewaters such as dairy barn flush water. The physical media of packed-beds retain biofilms, often allowing increased treatment rates. Previous studies have evaluated several types of media for digestion of dilute wastewaters, but cost and media fouling have setback commercial development. A major operational cost has been effluent recirculation pumping.

In the present effort, a novel approach to anaerobic digestion of flush dairy water was developed at pilot-scale: broken walnut shells were used as a low-cost packed-bed medium and effluent recirculation was replaced …

The Effects Of Hmtba (2-Hydroxy-4-Methylthio-Butanoic Acid) Supplementation On Ruminal Microbial Crude Protein Synthesis And Community Structure In Dairy Cattle, Chad James Robert Jenkins

Department of Animal Science: Dissertations, Theses, and Student Research

Metabolizable protein (MP) is protein that reaches the small intestine and is available for absorption and utilization by the cow. Dairy rations may be limited in the supply of MP essential to meeting the demands of milk synthesis, however as much as half of the MP flowing to the small intestine may be attributed to microbial origins and is referred to as microbial CP (MCP). Experiment 1 utilized a technique in which DNA was used as a microbial marker to estimate the concentration of bacterial CP (BCP) in the solid and liquid portions of rumen digesta. Rumen digesta was sampled …

Efficient Gene Targeting In Golden Syrian Hamsters By The Crispr/Cas9 System, Zhiqiang Fan, Wei Li, Sang R. Lee, Qinggang Meng, Tom D. Bunch, Kenneth L. White, Zhongde Wang, Il-Keun Kong

Animal, Dairy, and Veterinary Science Faculty Publications

The golden Syrian hamster is the model of choice or the only rodent model for studying many human diseases. However, the lack of gene targeting tools in hamsters severely limits their use in biomedical research. Here, we report the first successful application of the CRISPR/Cas9 system to efficiently conduct gene targeting in hamsters. We designed five synthetic single-guide RNAs (sgRNAs)—three for targeting the coding sequences for different functional domains of the hamster STAT2 protein, one for KCNQ1, and one for PPP1R12C—and demonstrated that the CRISPR/Cas9 system is highly efficient in introducing site-specific mutations in hamster somatic cells. We …

Effect Of Conditioned Medium On The Recovery Of Dormant Mycobacteria In Culture, Kelly E. Rock

Journal of Undergraduate Research at Minnesota State University, Mankato

Mycobacterium avium subsp. paratuberculosis (Mpt) is the etiologic agent of Johne’s disease, a chronic intestinal disease in cattle that threatens the economic viability of dairy farming. Diagnostic culture is typically unrewarding until several years after infection when clinical signs can be observed. This leads to widespread infection within the herd. Difficulty in culturing Mpt may be a result of oxidative damage due to the increased metabolic rate when dormant organisms are recovered in a nutrient rich medium. In order to improve recovery it is believed that some organisms secrete a growth factor in times of environmental stress which enables them …

Choline And Choline Metabolite Patterns And Associations In Blood And Milk During Lactation In Dairy Cows, Virginia M. Artegoitia, Jesse L. Foote, Federico M. Harte, Shawn R. Campagna, Michael J. De Veth

Department of Animal Science: Faculty Publications

Milk and dairy products are an important source of choline, a nutrient essential for human health. Infant formula derived from bovine milk contains a number of metabolic forms of choline, all contribute to the growth and development of the newborn. At present, little is known about the factors that influence the concentrations of choline metabolites in milk. The objectives of this study were to characterize and then evaluate associations for choline and its metabolites in blood and milk through the first 37 weeks of lactation in the dairy cow. Milk and blood samples from twelve Holstein cows were collected in …

Energy Content Of Reduced-Fat Dried Distillers’ Grains And Solubles For Lactating Dairy Cows And Effects On Energy And Nitrogen Balance, Alison J. Foth

Department of Animal Science: Dissertations, Theses, and Student Research

Eight Holstein and 8 Jersey multiparous, lactating cows were used to complete 56 energy balances to determine the energy content of reduced-fat distillers grains and solubles (RFDDGS). A repeated switchback design was used to compare treatments with and without RFDDGS. Diets consisted of 24.2 % corn silage, 18.4 % alfalfa hay, 6.94 % brome hay with either 22.9 % rolled corn and 14.8 % soybean meal (Control), or 8.95 % rolled corn, 28.8 % RFDDGS, and 0 % soybean meal (Co-P; DM basis). The inclusion of RFDDGS did not affect (P = 0.86) DMI averaging 21.4 ± 0.53 kg …

2013 Annual Report, Various Authors

Annual Reports

No abstract provided.

The Effects Of Different Cryopreservation Techniques On The Survival Rate Of In Vitro Produced (Ivp) And Biopsied Bovine Blastocysts, Diana J. Gumber

Animal Science

This study’s objectives were to determine if the biopsy of expanded blastocysts prior to cryopreservation affected the survival rates and determine if there is a difference between slow-freezing in 10% glycerol, slow freezing in ethylene glycol, and vitrification in terms of survival rate of the embryos. Blastocoeles were reduced prior to cryopreservation by means of biopsy. Embryos for slow freezing were exposed to ethylene glycol (SynGRO Ethylene Glycol with Sucrose, BIONICHE, Pullman, WA, USA) or 10% glycerol (SynGRO 10% Glycerol, BIONICHE, Pullman, WA, USA) as cryoprotectants. Vitrified embryos were exposed to 10% and 40% ethylene glycol. Survival rate of biopsied …

Ghrelin Concentrations In Milk And Plasma Of Dairy Cows During Early Lactation, Sameer M. Alhojaily

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ghrelin is a hormone produced mainly by the cells lining the gastric mucosa. Ghrelin was first extracted from human and rat stomachs, and identified as an endogenous stimulator of growth hormone release. Ghrelin is synthesized and produced in several tissues, but the gastric mucosa remains the major source of circulating ghrelin. Besides growth hormone release, ghrelin stimulates appetite and plays some major roles in different organs. In several studies, ghrelin was described as a hormone with multiple functions and diverse biological actions. Ghrelin exists in two major forms, active ghrelin and inactive ghrelin, and only the active from binds to …

Physiological Impacts And Lactational Performance Of Dairy Cows Fed Brown Midrib Corn Silage During Dry Period Through Early To Midlactation, Alexandra Windley Kelley

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Developing solutions to the metabolic stress experienced by cows during the transition period is very important because it can negatively influence lactational performance. The objectives were to: 1) compare physiological changes through body weight (BW) and concentrations of non-esterified fatty acids (NEFA) and β-hydroxybutyric acid (BHBA) and 2) evaluate feed intake, milk production, and energy balance (EB) of cows fed brown midrib corn silage (BMRCS)-based diets when compared with conventional corn silage (CCS)-based diets during the transition. At 4 wk prior to parturition, 40 dry multiparous Holstein cows …

Methods Of Bovine Reproduction, Kelsey L. O'Donnell

Honors Scholar Theses

The beef and dairy cattle industries are of immense economic value in the United States and have made impressive advancements over the past century. Their success depends strongly upon the reproductive efficiency of their breeding programs. This overview discusses the major advancements in bovine reproduction that have been integrated into the beef and dairy cattle industries and also the emerging technologies that are currently being researched. Current practices like artificial insemination and estrus synchronization resulted from the development of a strong understanding of cattle physiology and the endocrinology of the estrous cycle. With these technologies, selection of superior genetic traits …

Tracking Heat-Resistant, Sporeforming Bacteria In The Milk Chain: A Farm To Table Approach, Maricarmen Estrada Anzueto

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Sporeforming bacteria (such as Bacillus and Paenibacillus spp.) can survive pasteurization conditions (Collins, 1981) and grow in pasteurized fluid milk during refrigerated storage (Huck et al., 2008; Ivy et al., 2012), causing fluid milk spoilage and limiting the further extension of fluid milk’s shelf life (Fromm and Boor, 2004; Durak et al., 2006). Moreover, Bacillus and related genera have been found in raw milk, pasteurized milk and environmental samples from dairy farms, indicating that these organisms are ubiquitous in nature and can enter the milk chain from different sources (Huck et al., 2007b; Huck et al., 2008; Ranieri and Boor, …

Halogeton (H. Glomeratus) Poisoning In Cattle: Case Report, Kerry A. Rood, Kip E. Panter, Dale R. Gardner, Bryan L. Stegelmeier, Jeffery O. Hall

Animal, Dairy, and Veterinary Science Faculty Publications

Historically, the most significant losses from halogeton poisoning have been reported in sheep, with multiple catastrophic deaths documented. While recorded death losses in cattle from halogeton poisoning are less common than in sheep, recent cases, including 2 reported here, and anecdotal reports from other ranchers suggest that the impact of halogeton losses in cattle herds in the western United States is much more widespread than originally thought. Halogeton may accumulate up to 30% oxalates; a small amount of the plant (300 g) is enough to cause death in sheep. Oxalates precipitate calcium from the blood, resulting in hypocalcemia, formation of …

P-28 The Prevalence Of Encysted Toxoplasma & Sarcocystis In Consumer-Grade Pork, Beef, And Mutton In Michiana, Ross Trecartin

Honors Scholars & Undergraduate Research Poster Symposium Programs

Commonly present in the muscle of animals are the tissue cysts of Toxoplasma and Sarcocystis. The aim of this study was to determine the presence and levels of Toxoplasma/Sarcocystis tissue cysts in retail pork, beef, and mutton throughout the Michiana area. A total of 36 samples, 12 from each species, were exposed to a digestive solution mimicking stomach conditions. The solution consisted of Pepsin 0.75% , NaCl 0.86%, and HCl adjusted to a pH of 1-2. The digested samples were then strained through several layers of cheesecloth, centrifuged, and examined for the presence of parasites.

Triple Immunoglobulin Gene Knockout Transchromosomic (Tc) Cattle: Bovine Lambda Cluster Deletion And Its Effect On Fully Human Polyclonal Antibody Production, H. Matsushita, A. Sano, H. Wu, J. Jiao, P. Kasinathan, E. J. Sullivan, Zhongde Wang, K. Kuroiwa

Animal, Dairy, and Veterinary Science Faculty Publications

Towards the goal of producing fully human polyclonal antibodies (hpAbs or hIgGs) in transchromosomic (Tc) cattle, we previously reported that Tc cattle carrying a human artificial chromosome (HAC) comprising the entire unrearranged human immunoglobulin (Ig) heavy-chain (hIGH), kappa-chain (hIGK), and lambda-chain (hIGL) germline loci produced physiological levels of hIgGs when both of the bovine immunoglobulin mu heavy-chains, bIGHM and bIGHML1, were homozygously inactivated (bIGHM-/-, bIGHML1-/-; double knockouts or DKO). However, because endogenous bovine immunoglobulin light chain loci are still intact, the light chains are produced both from the hIGK and hIGL genomic loci on the HAC and from the endogenous …

Effects Of Covering System, Sealing Time, And Packing On Fermentation, Nutritional Quality, And Organic Matter Loss Of Corn Silage In A Drive-Over Pile, Katie Rose Nutcher

Dairy Science

This project involved an experiment conducted using a 550-ton capacity drive-over pile of whole-plant corn silage. The main effects compared were: packing the final forage surface with a loader or crawler, delay or immediate sealing, and covering with standard plastic or an oxygen barrier film.

Numerous studies have shown that the absence of oxygen in silage stored in a bunker silo or pile is crucial for proper fermentation and to ensure the highest quality silage at feed out. When oxygen is allowed to permeate through the covering material, it leads to visible spoilage. This trial showed that the oxygen barrier …

A Recommendation For The California Dairy System Reform Based On A Comparative Study Of Dairy Market Structuring And Pricing Systems Of Seven Leading Dairy Industries In 2014., Meredith Frisius

Dairy Science

No abstract provided.

Best Management Practices For Raising Dairy Calves From Birth To Weaning, Alexa Cabral

Dairy Science

There are many different ways to raise calves on dairy farms in the United States. The way calves are raised can depend on the resources, natural environment and operation of the farm. There is no right or wrong way to raise calves. However, the aim of raising calves is to find the best way to raise a healthy calf so that she will become a productive cow. The objective of this literature review was to determine the ideal management practices for raising a healthy calf from birth to weaning. This literature analyzed numerous articles that covered all aspects of calf …

Preparing Samples Of Rancid Milk For Milk Judging Contests, Danielle Li

Dairy Science

Due to the growing concern of raw milk, there is a growing need for other methods of preparing rancid milk for milk judging. The origin of rancidity in milk is due to lipolysis caused the lipase enzyme. However, with pasteurization, the lipase enzyme is inactivated. The purpose of this experiment was to compare the effect of using lipase powder to other current methods of preparing rancid milk, such as utilizing raw milk. Result showed that all treatments caused desired rancidity. The second purpose was to compare the rancidity between Method A, two to three hour incubation at room temperature, and …

Development Of Applied Standard Operating Procedures And Monitoring Practices, Nick Southfield

Dairy Science

Development of a standard operating procedure program for Southfield Dairy was chosen in order to improve the work environment and animal health with an underlying goal to improve profitability. Critical control points for the milk barns, maternity barn and calf barn on the dairy, were identified as being critical to the production of quality milk, both for sale and calf feed. Data recording implementation through identification of recordable data, equipment purchases, and log sheet production, was done so to increase relevant information available for veterinary services and specialty consultants. Implementation of procedural documents and training programs are discussed to see …

A Literature Review On Injection-Site Lesions In Dairy Cows And Culling Decisions, Eric B. Parsons

Dairy Science

Currently dairy producers face particular problems concerning their culled cows and bulls, these problems must be addressed so dairy producers have the opportunity to improve the quality of beef produced from their cull dairy cows. At the moment, not much is known about culling decisions outside “break-even” numbers and poor cow conditioning. This literature review outlines the strategy behind culling decisions and looks into potential plans to develop progressive body conditioning and quality of meat sent to auction. Currently the dairy industry is not placing much demand into providing the livestock auctions and harvest plants with higher quality meat. This …

Using Progesterone As An Indicator Of Ovarian Response To Stimulation In Cattle, Kayla Mariah Kauffman

Animal Science

No abstract provided.

The Implications Of Global Warming Upon United States Dairy Herds: Effects On The Economics Of Dairying And Stability Of Herds With Low Genetic Diversity, Lauren Lusby

Dairy Science

Global warming (GW) is of real concern for the current U.S. agricultural industry. The agricultural industry has had a hand in emitting into the atmosphere. Severe heat spells seen nationwide and volatile climate shifts have pushed states such as California to implement governmental support to high impact areas due to dwindling water sources. Motivated dairy industry members have created plans to reduce dairy GHE contribution. Some research has suggested a benefit in GHG emissions from fewer cow-higher production approaches to dairy farmers. Most U.S. dairy cattle are specialized due to high inbreeding values created by selecting for few production traits …

Effects Of Alternative Protein Milk Replacer Versus All-Milk Protein Milk Replacer On Weight Gain And Grain Intake In Pre-Weaned Dairy Calves, Kayla Sandoval

Dairy Science

The objective of this study was to determine if there was a significant difference in weight gain and grain intake between Holstein calves fed milk replacer with an alternative protein source and an all-milk replacer. The study took place on a large commercial calf operation in the Central Valley of California. All calves on trial were Holstein bull calves and started the trial within three to ten days of birth. Calves on trial were weighed a total of four times. The 236 treatments did not start until the second weight was measured at about 30 days of age and was …

Haccp-Based Program For Central Coast Creamery: A Small-Scale Artisan Cheese Production Facility, Catherine Machado

Dairy Science

This project was designed to outline a HACCP-based program that could be used for the start-up of Central Coast Creameries personal plan. The goal was to first provide a layout to more easily distinguish the hazards that the plant might face. After doing so the HACCP plan was made to eliminate those hazards both efficiently and successfully.

The Analysis Of Solar Photovoltaic Power System On A Large Central Valley Dairy Farm, Brogan Rose Fernandes

Dairy Science

The objective of this paper was to determine the financial benefits of installing a PV solar system on a dairy farm in California to create a more efficient and profitable business. The data used was based off Fernoak Farms electrical bills from Southern California Edison before and after solar installation. The background knowledge of how PV solar systems work and their design was incorporated, along with the research of the past, present, and future solar markets. The characteristics of energy consumption on an average dairy farm is given to show the amount of electricity needed to keep the dairy facility …

The Effects Of Implementing Cow Cooling For Oostdam Dairy: Benefits From Milk Production, Feed Intake, And Reproduction, Mark Oostdam

Dairy Science

Heat stress has a major effect on management decisions for a dairy operation because of the decrease in the cows productivity. Dairy cattle loose efficiency of economic production due to lower milk yields and decreased pregnancy rates. Milk loss due to heat stressed cows in California averages a loss of 293 kg of milk/cow/year(St.-Pierre et al., 2003), and with milk prices reaching record highs, dairies are losing out on increased revenue opportunity. Increases in reproductive infertility from heat stress also affect revenue for a dairy operation by reducing conception rates (Hansen 1997) and pregnancy rates, which causes the average days …

Improving Cow Comfort To Reduce Health Issues And Increase Milk Yield On Dairy Farms, Shonlyn Ferrari

Dairy Science

In recent years the dairy industry has been looking more and more into cow comfort. This is because there are many benefits to ensuring cows are in some of the best new facilities. I looked into some of the major issues relating to cow comfort which include how to improve your own dairy, what health problems are common with inadequate cow comfort, and how these issues relate back to milk yield. First cow comfort is outlined and explained, then health problems are shown to be caused by improper cow care and lastly how cow comfort can improve milk yield. In …

Setting Up A Safety Program For California Dairies., Shelby Locke

Dairy Science

The purpose of this project was to take the best research for dairy safety in California and combine it in everyday terminology that the average dairy producer could understand. In today’s dairy industry, money is tight and decreasing costs is very important. By implementing a safety program that is in compliance with CAL/OSHA’s standards, injuries will decrease, saving the dairy producer money. This paper has highlighted the major topics for a new dairy to start their safety training program. It includes descriptions of the hazards, responsibilities for employers and employees and ways to prevent accidents from occurring. By summarizing the …

The Effects Of Substituting True Protein With Non-Protein Nitrogen In Holstein Dairy Heifers Precision-Fed Different Forage To Concentrate Ratios, Noe Alberto Gomez

Animal Science

Understanding the efficiency and digestibility of dietary nutrients in dairy heifers is essential for minimizing costs and environmental impacts of the dairy industry. The objective of this study was to interpret the effects of manipulating rumen-degradable protein (RDP) in a precision feeding system. Eight Holstein ruminally cannulated heifers (14.6 ± 0.1 mo of age, and 386 ± 9.1 kg of weight) were randomly assigned to 2 forage levels: HC (25% forage) and LC (75% forage) and to a degradable protein sequence [0% degradable protein from urea U, 100% casein C (no urea treatment U0); 33% U, 67% C …