Dairy Science Commons^™

Absorption And Utilization Of Choline And Vitamin B12 In Lactating Dairy Cows Using Different Delivery Methods, Virginia Maria Artegoitia Etcheverry

Doctoral Dissertations

Choline and vitamin B12 are essential nutrients for growth and performances of production animals. However, both nutrients are extensively degraded during digestion in the rumen. This thesis comprised three experiments. First, four cows equipped with a rumen cannula and catheters in the portal vein and a mesenteric artery received a post-ruminal bolus of: 1) cyanocobalamin (CN-CBL) alone (0.1 g) [gram], 2) CN-CBL (0.1 g) + casein (10 g) or 3) CN-CBL (0.1 g) + whey proteins (10 g). After the bolus, blood samples were taken until 24 h [hour] post-bolus. The intestinal absorption of CN-CBL was greater when the vitamin …

Methane Production By A Packed-Bed Anaerobic Digester Fed Dairy Barn Flush Water, Sean Richard Thomson

Master's Theses

Packed-bed digesters are an alternative to covered lagoon digesters for methane production and anaerobic treatment of dilute wastewaters such as dairy barn flush water. The physical media of packed-beds retain biofilms, often allowing increased treatment rates. Previous studies have evaluated several types of media for digestion of dilute wastewaters, but cost and media fouling have setback commercial development. A major operational cost has been effluent recirculation pumping.

In the present effort, a novel approach to anaerobic digestion of flush dairy water was developed at pilot-scale: broken walnut shells were used as a low-cost packed-bed medium and effluent recirculation was replaced …

The Effects Of Hmtba (2-Hydroxy-4-Methylthio-Butanoic Acid) Supplementation On Ruminal Microbial Crude Protein Synthesis And Community Structure In Dairy Cattle, Chad J. R. Jenkins

Department of Animal Science: Dissertations, Theses, and Student Research

Metabolizable protein (MP) is protein that reaches the small intestine and is available for absorption and utilization by the cow. Dairy rations may be limited in the supply of MP essential to meeting the demands of milk synthesis, however as much as half of the MP flowing to the small intestine may be attributed to microbial origins and is referred to as microbial CP (MCP). Experiment 1 utilized a technique in which DNA was used as a microbial marker to estimate the concentration of bacterial CP (BCP) in the solid and liquid portions of rumen digesta. Rumen digesta was sampled …

Efficient Gene Targeting In Golden Syrian Hamsters By The Crispr/Cas9 System, Zhiqiang Fan, Wei Li, Sang R. Lee, Qinggang Meng, Tom D. Bunch, Kenneth L. White, Zhongde Wang, Il-Keun Kong

Animal, Dairy, and Veterinary Science Faculty Publications

The golden Syrian hamster is the model of choice or the only rodent model for studying many human diseases. However, the lack of gene targeting tools in hamsters severely limits their use in biomedical research. Here, we report the first successful application of the CRISPR/Cas9 system to efficiently conduct gene targeting in hamsters. We designed five synthetic single-guide RNAs (sgRNAs)—three for targeting the coding sequences for different functional domains of the hamster STAT2 protein, one for KCNQ1, and one for PPP1R12C—and demonstrated that the CRISPR/Cas9 system is highly efficient in introducing site-specific mutations in hamster somatic cells. We …

Effect Of Conditioned Medium On The Recovery Of Dormant Mycobacteria In Culture, Kelly E. Rock

Journal of Undergraduate Research at Minnesota State University, Mankato

Mycobacterium avium subsp. paratuberculosis (Mpt) is the etiologic agent of Johne’s disease, a chronic intestinal disease in cattle that threatens the economic viability of dairy farming. Diagnostic culture is typically unrewarding until several years after infection when clinical signs can be observed. This leads to widespread infection within the herd. Difficulty in culturing Mpt may be a result of oxidative damage due to the increased metabolic rate when dormant organisms are recovered in a nutrient rich medium. In order to improve recovery it is believed that some organisms secrete a growth factor in times of environmental stress which enables them …

Casein Micelles And Their Properties: Polydispersity, Association With Vitamin A And Effect Of Ultra-High Pressure Homogenization, Maneesha Sheenu Mohan

Doctoral Dissertations

The internal structure of casein micelles is not fully understood. In the present work, we explore some of the basic questions about casein micelles in bovine milk including its size distribution, native binding properties and effect of ultra-high pressure homogenization (ultra-HPH) on technological properties of casein micelles. The size distribution of casein micelles was studied by cryo-TEM using skim milk from four cows. The degree of variation in casein micelle sizes (polydispersity) ranged between 0.39 and 0.47 for a size distribution ranging from 10 to 693 nm. These results indicated high polydispersity of casein micelles in milk from single cows. …

Choline And Choline Metabolite Patterns And Associations In Blood And Milk During Lactation In Dairy Cows, Virginia M. Artegoitia, Jesse L. Foote, Federico M. Harte, Shawn R. Campagna, Michael J. De Veth

Department of Animal Science: Faculty Publications

Milk and dairy products are an important source of choline, a nutrient essential for human health. Infant formula derived from bovine milk contains a number of metabolic forms of choline, all contribute to the growth and development of the newborn. At present, little is known about the factors that influence the concentrations of choline metabolites in milk. The objectives of this study were to characterize and then evaluate associations for choline and its metabolites in blood and milk through the first 37 weeks of lactation in the dairy cow. Milk and blood samples from twelve Holstein cows were collected in …

Impact Of Matrix Metallopeptidase-9 Supplementation During In Vitro Maturation Of Bovine Oocytes, Megan Rene Goodwin

Masters Theses

Heat-induced decreases in bovine blastocyst development have been related to reductions in latent matrix metallopeptidase-9 (proMMP9) production from maturing cumulus-oocyte complexes. Elevated intrafollicular proMMP9 levels at the time of oocyte retrieval have been positively related to pregnancy following human IVF. Thus, we hypothesized that heat-induced reductions in proMMP9 levels during oocyte maturation may be responsible for decreased blastocyst development. As a first step towards testing this hypothesis, bovine cumulus-oocyte complexes were matured at 38.5°C for 24 h with 0 or 300 ng/mL recombinant human proMMP9 (rhMMP9) added at 0 h of in vitro maturation (hIVM). No differences were found in …

Energy Content Of Reduced-Fat Dried Distillers Grains And Solubles For Lactating Dairy Cows And Effects On Energy And Nitrogen Balance, Alison J. Foth

Department of Animal Science: Dissertations, Theses, and Student Research

Eight Holstein and 8 Jersey multiparous, lactating cows were used to complete 56 energy balances to determine the energy content of reduced-fat distillers grains and solubles (RFDDGS). A repeated switchback design was used to compare treatments with and without RFDDGS. Diets consisted of 24.2 % corn silage, 18.4 % alfalfa hay, 6.94 % brome hay with either 22.9 % rolled corn and 14.8 % soybean meal (Control), or 8.95 % rolled corn, 28.8 % RFDDGS, and 0 % soybean meal (Co-P; DM basis). The inclusion of RFDDGS did not affect (P = 0.86) DMI averaging 21.4 ± 0.53 kg …

The Effects Of Different Cryopreservation Techniques On The Survival Rate Of In Vitro Produced (Ivp) And Biopsied Bovine Blastocysts, Diana J. Gumber

Animal Science

This study’s objectives were to determine if the biopsy of expanded blastocysts prior to cryopreservation affected the survival rates and determine if there is a difference between slow-freezing in 10% glycerol, slow freezing in ethylene glycol, and vitrification in terms of survival rate of the embryos. Blastocoeles were reduced prior to cryopreservation by means of biopsy. Embryos for slow freezing were exposed to ethylene glycol (SynGRO Ethylene Glycol with Sucrose, BIONICHE, Pullman, WA, USA) or 10% glycerol (SynGRO 10% Glycerol, BIONICHE, Pullman, WA, USA) as cryoprotectants. Vitrified embryos were exposed to 10% and 40% ethylene glycol. Survival rate of biopsied …

2013 Annual Report, Various Authors

Annual Reports

No abstract provided.

Evaluating Colostrum Quality With Niacin Supplementation In The Diet Of Dairy Cattle, Allison Marie Pike

Student Research Projects

Dairy calves are born with a naive immune system, and must obtain passive immunity through ingestion of colostrum. Colostrum provides the newborn with antimicrobial proteins called immunoglobulins, such as immunoglobulin G (IgG), that protect the calf for the first few weeks of life. Calves that receive good quality colostrum (>50 g/L of IgG) are more likely to survive to adulthood. Niacin is a vasodilator that may increase blood flow to the mammary gland during production of colostrum in the mother. This experiment investigated the effect of niacin supplementation at a rate of 48 g/cow/day for 3 weeks before calving …

Genetic Resistance To Staphylococcus Aureus Mastitis Associated With Bovine Cxcr1, Olivia Jean Crisp

Chancellor’s Honors Program Projects

No abstract provided.

Physiological Impacts And Lactational Performance Of Dairy Cows Fed Brown Midrib Corn Silage During Dry Period Through Early To Midlactation, Alexandra Windley Kelley

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Developing solutions to the metabolic stress experienced by cows during the transition period is very important because it can negatively influence lactational performance. The objectives were to: 1) compare physiological changes through body weight (BW) and concentrations of non-esterified fatty acids (NEFA) and β-hydroxybutyric acid (BHBA) and 2) evaluate feed intake, milk production, and energy balance (EB) of cows fed brown midrib corn silage (BMRCS)-based diets when compared with conventional corn silage (CCS)-based diets during the transition. At 4 wk prior to parturition, 40 dry multiparous Holstein cows …

The Effect Of Bedding Surface And Muscid Fly Populations On The Welfare Of Pre Weaned Holstein And Jersey Calves, Christa Anne Kurman

Masters Theses

The first objective of this study was to determine the effect of bedding on the welfare of Holstein and Jersey calves housed using individual hutches bedded with gravel, rubber mats, or sand. A second objective was to determine the effects of stable and house fly populations on calf welfare. Bedding quality and fly management are aspects of improving animal welfare on dairy farms. It was hypothesized that sand or mat would increase lying time and decrease lying bouts, but not affect BW gain or feed intake in calves indicating that these bedding surfaces increased calf welfare. It was hypothesized that …

Ghrelin Concentrations In Milk And Plasma Of Dairy Cows During Early Lactation, Sameer M. Alhojaily

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ghrelin is a hormone produced mainly by the cells lining the gastric mucosa. Ghrelin was first extracted from human and rat stomachs, and identified as an endogenous stimulator of growth hormone release. Ghrelin is synthesized and produced in several tissues, but the gastric mucosa remains the major source of circulating ghrelin. Besides growth hormone release, ghrelin stimulates appetite and plays some major roles in different organs. In several studies, ghrelin was described as a hormone with multiple functions and diverse biological actions. Ghrelin exists in two major forms, active ghrelin and inactive ghrelin, and only the active from binds to …

The Spread And Potential Control Of Disease Across The Domestic Cattle-Wildlife Interface, Rachel Jackson

Chancellor’s Honors Program Projects

No abstract provided.

Methods Of Bovine Reproduction, Kelsey L. O'Donnell

Honors Scholar Theses

The beef and dairy cattle industries are of immense economic value in the United States and have made impressive advancements over the past century. Their success depends strongly upon the reproductive efficiency of their breeding programs. This overview discusses the major advancements in bovine reproduction that have been integrated into the beef and dairy cattle industries and also the emerging technologies that are currently being researched. Current practices like artificial insemination and estrus synchronization resulted from the development of a strong understanding of cattle physiology and the endocrinology of the estrous cycle. With these technologies, selection of superior genetic traits …

Tracking Heat-Resistant, Sporeforming Bacteria In The Milk Chain: A Farm To Table Approach, Maricarmen Estrada Anzueto

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Sporeforming bacteria (such as Bacillus and Paenibacillus spp.) can survive pasteurization conditions (Collins, 1981) and grow in pasteurized fluid milk during refrigerated storage (Huck et al., 2008; Ivy et al., 2012), causing fluid milk spoilage and limiting the further extension of fluid milk’s shelf life (Fromm and Boor, 2004; Durak et al., 2006). Moreover, Bacillus and related genera have been found in raw milk, pasteurized milk and environmental samples from dairy farms, indicating that these organisms are ubiquitous in nature and can enter the milk chain from different sources (Huck et al., 2007b; Huck et al., 2008; Ranieri and Boor, …

Halogeton (H. Glomeratus) Poisoning In Cattle: Case Report, Kerry A. Rood, Kip E. Panter, Dale R. Gardner, Bryan L. Stegelmeier, Jeffery O. Hall

Animal, Dairy, and Veterinary Science Faculty Publications

Historically, the most significant losses from halogeton poisoning have been reported in sheep, with multiple catastrophic deaths documented. While recorded death losses in cattle from halogeton poisoning are less common than in sheep, recent cases, including 2 reported here, and anecdotal reports from other ranchers suggest that the impact of halogeton losses in cattle herds in the western United States is much more widespread than originally thought. Halogeton may accumulate up to 30% oxalates; a small amount of the plant (300 g) is enough to cause death in sheep. Oxalates precipitate calcium from the blood, resulting in hypocalcemia, formation of …

P-28 The Prevalence Of Encysted Toxoplasma & Sarcocystis In Consumer-Grade Pork, Beef, And Mutton In Michiana, Ross Trecartin

Honors Scholars & Undergraduate Research Poster Symposium Programs

Commonly present in the muscle of animals are the tissue cysts of Toxoplasma and Sarcocystis. The aim of this study was to determine the presence and levels of Toxoplasma/Sarcocystis tissue cysts in retail pork, beef, and mutton throughout the Michiana area. A total of 36 samples, 12 from each species, were exposed to a digestive solution mimicking stomach conditions. The solution consisted of Pepsin 0.75% , NaCl 0.86%, and HCl adjusted to a pH of 1-2. The digested samples were then strained through several layers of cheesecloth, centrifuged, and examined for the presence of parasites.

Triple Immunoglobulin Gene Knockout Transchromosomic (Tc) Cattle: Bovine Lambda Cluster Deletion And Its Effect On Fully Human Polyclonal Antibody Production, H. Matsushita, A. Sano, H. Wu, J. Jiao, P. Kasinathan, E. J. Sullivan, Zhongde Wang, K. Kuroiwa

Animal, Dairy, and Veterinary Science Faculty Publications

Towards the goal of producing fully human polyclonal antibodies (hpAbs or hIgGs) in transchromosomic (Tc) cattle, we previously reported that Tc cattle carrying a human artificial chromosome (HAC) comprising the entire unrearranged human immunoglobulin (Ig) heavy-chain (hIGH), kappa-chain (hIGK), and lambda-chain (hIGL) germline loci produced physiological levels of hIgGs when both of the bovine immunoglobulin mu heavy-chains, bIGHM and bIGHML1, were homozygously inactivated (bIGHM-/-, bIGHML1-/-; double knockouts or DKO). However, because endogenous bovine immunoglobulin light chain loci are still intact, the light chains are produced both from the hIGK and hIGL genomic loci on the HAC and from the endogenous …

Effects Of Covering System, Sealing Time, And Packing On Fermentation, Nutritional Quality, And Organic Matter Loss Of Corn Silage In A Drive-Over Pile, Katie Rose Nutcher

Dairy Science

This project involved an experiment conducted using a 550-ton capacity drive-over pile of whole-plant corn silage. The main effects compared were: packing the final forage surface with a loader or crawler, delay or immediate sealing, and covering with standard plastic or an oxygen barrier film.

Numerous studies have shown that the absence of oxygen in silage stored in a bunker silo or pile is crucial for proper fermentation and to ensure the highest quality silage at feed out. When oxygen is allowed to permeate through the covering material, it leads to visible spoilage. This trial showed that the oxygen barrier …

Improving Cow Comfort To Reduce Health Issues And Increase Milk Yield On Dairy Farms, Shonlyn Ferrari

Dairy Science

In recent years the dairy industry has been looking more and more into cow comfort. This is because there are many benefits to ensuring cows are in some of the best new facilities. I looked into some of the major issues relating to cow comfort which include how to improve your own dairy, what health problems are common with inadequate cow comfort, and how these issues relate back to milk yield. First cow comfort is outlined and explained, then health problems are shown to be caused by improper cow care and lastly how cow comfort can improve milk yield. In …

Setting Up A Safety Program For California Dairies., Shelby Locke

Dairy Science

The purpose of this project was to take the best research for dairy safety in California and combine it in everyday terminology that the average dairy producer could understand. In today’s dairy industry, money is tight and decreasing costs is very important. By implementing a safety program that is in compliance with CAL/OSHA’s standards, injuries will decrease, saving the dairy producer money. This paper has highlighted the major topics for a new dairy to start their safety training program. It includes descriptions of the hazards, responsibilities for employers and employees and ways to prevent accidents from occurring. By summarizing the …

Effects Of Alternative Protein Milk Replacer Versus All-Milk Protein Milk Replacer On Weight Gain And Grain Intake In Pre-Weaned Dairy Calves, Kayla Sandoval

Dairy Science

The objective of this study was to determine if there was a significant difference in weight gain and grain intake between Holstein calves fed milk replacer with an alternative protein source and an all-milk replacer. The study took place on a large commercial calf operation in the Central Valley of California. All calves on trial were Holstein bull calves and started the trial within three to ten days of birth. Calves on trial were weighed a total of four times. The 236 treatments did not start until the second weight was measured at about 30 days of age and was …

A Recommendation For The California Dairy System Reform Based On A Comparative Study Of Dairy Market Structuring And Pricing Systems Of Seven Leading Dairy Industries In 2014., Meredith Frisius

Dairy Science

No abstract provided.

Best Management Practices For Raising Dairy Calves From Birth To Weaning, Alexa Cabral

Dairy Science

There are many different ways to raise calves on dairy farms in the United States. The way calves are raised can depend on the resources, natural environment and operation of the farm. There is no right or wrong way to raise calves. However, the aim of raising calves is to find the best way to raise a healthy calf so that she will become a productive cow. The objective of this literature review was to determine the ideal management practices for raising a healthy calf from birth to weaning. This literature analyzed numerous articles that covered all aspects of calf …

Preparing Samples Of Rancid Milk For Milk Judging Contests, Danielle Li

Dairy Science

Due to the growing concern of raw milk, there is a growing need for other methods of preparing rancid milk for milk judging. The origin of rancidity in milk is due to lipolysis caused the lipase enzyme. However, with pasteurization, the lipase enzyme is inactivated. The purpose of this experiment was to compare the effect of using lipase powder to other current methods of preparing rancid milk, such as utilizing raw milk. Result showed that all treatments caused desired rancidity. The second purpose was to compare the rancidity between Method A, two to three hour incubation at room temperature, and …

Haccp-Based Program For Central Coast Creamery: A Small-Scale Artisan Cheese Production Facility, Catherine Machado

Dairy Science

This project was designed to outline a HACCP-based program that could be used for the start-up of Central Coast Creameries personal plan. The goal was to first provide a layout to more easily distinguish the hazards that the plant might face. After doing so the HACCP plan was made to eliminate those hazards both efficiently and successfully.