Dairy Science Commons^™

Crop/Livestock Integration Effects On Soil Quality, Crop Production, And Soil Nitrogen Dynamics, Ellen B. Mallory

Electronic Theses and Dissertations

Regional integration of potato and dairy farms has developed in Maine through arrangements where manure, feed, and sometimes land, are exchanged between neighboring farms. The effects of integration on soil quality, crop production, nitrogen (N) cycling, and N loss were investigated in field and laboratory studies of contrasting amended (manure, compost, green manure, and supplemental fertilizer) and nonamended (fertilizer only) soil management systems within 2-year potato (Solanum tuberosum L.) rotations in the Maine Potato Cropping Systems Project (MPEP). Additionally, soil quality of 48 integrated and nonintegrated Maine potato and dairy farm fields was assessed. The MPEP’s amended soil system enhanced …

Effect Of Processing On The Composition And Microstructure Of Buttermilk And Its Milk Fat Globule Membranes, Pierre Morin, Rafael Jiménez-Flores, Yves Pouliot

Dairy Science

The effect of cream pasteurization on the composition and microstructure of buttermilk after pasteurization, evaporation and spray-drying was studied. The composition of milk fat globule membrane (MFGM) isolated from buttermilk samples was also characterized. Pasteurization of cream resulted in higher lipid recovery in the buttermilk. Spray-drying of buttermilk had a significant effect on phospholipid content and composition. After spray-drying, the phospholipid content decreased by 38.2% and 40.6%, respectively in buttermilk from raw or pasteurized cream when compared with initial buttermilks. Pasteurization of cream resulted in the highest increase in whey protein recovery in MFGM isolates compared with all other processing …

Evaluación De La Estabilidad Proteica En La Leche Y Su Relación Con La Calidad Microbiológica En Los Municipios De Tocancipá, Tabio Y Zipaquirá, Edgar Mauricio Ariza Aya

Zootecnia

Se realizó la evaluación de la estabilidad proteica por medio de la técnica de Ramsdell y la calidad higiénica de la leche a través de recuentos totales de mesofilos y coliformes, en 18 hatos de la sabana de Bogota, para establecer alguna relación entre estas dos variables evaluadas. El muestreo se organizó por rutas de recolección establecidas por la empresa Alquería S.A y se realizó durante los meses de noviembre de 2006 y enero del 2007, se empleó la técnica de recuento en placa para el recuento de Coliformes con el medio VRB y de Mesofilos con el medio Plate …

Interview With Melissa & Rod Volbeda, Willamette Valley Cheese, 2007 (Audio), Melissa Volbeda, Rod Volbeda

All Sustainability History Project Oral Histories

Interview of Melissa and Rod Volbeda by Magda Gaytan on August 6th, 2007.

Willamette Valley Cheese Company tour photos available for download.

Digestibility Of Limit Fed High And Low Concentrate Diets With Corn Silage As The Sole Forage For Dairy Heifers With Saccharomyces Cerevisiae, G. J. Lascano, A. J. Heinrichs

Dairy Science

No abstract provided.

Rumen Fermentation Patterns Of Dairy Heifers Fed Restricted Amounts Of High, Medium, And Low Concentrate Diets And The Addition Of Saccharomyces Cerevisiae, G. J. Lascano, A. J. Heinrichs

Dairy Science

No abstract provided.

Influence Of Free-Stall Base On Tarsal Joint Lesions And Hygiene In Dairy Cows, W. K. Fulwider, T. Grandin, D. J. Garrick, T. E. Engle, W. D. Lamm, N. L. Dalsted, B. E. Rollin

Housing and Confinement of Farm Animals Collection

The objective was to quantify the incidence of tarsal lesions and level of hygiene by stall bed type. Cows were scored on 100 dairies from Wisconsin, Minnesota, Indiana, Iowa, and New York in the fall and winter. Thirty-eight dairies used rubber-filled mattresses (RFM), 27 had sand beds, 29 had waterbeds, and 6 used compost packs (CPk). Stocking density, stall dimensions, bedding amount, bedding frequency, and type of bedding were recorded. One pen of early-lactation multiparous cows on each dairy was scored based on injury of the tarsal joints at the lateral and medial surfaces and tuber calcis at the dorsal, …

A Comparison Of Pyronin Y-Methyl Green Stain And Methylene Blue Stain For Somatic Cell Count In Sheep Milk, Emily Mirek, Stacey O’Donnell

Senior Honors Projects

Somatic cell count is a key method used to evaluate the quality of milk in today’s growing dairy sheep industry. Somatic cells are body cells, primarily the white blood cells, found in a milk sample. If an infection such as mastitis is present, the number of somatic cells in the milk increases (Gonzalo, et al. 1992). Producers routinely perform somatic cell counts on cow and goat milk. For dairy cattle in the United States, the somatic cell count can not exceed 750,000 cells per milliliter, for commercial milk. For dairy goats the accepted limit is 1,000,000 cells per mL of …

Investigating The Role Of Transcription Factor Ap-2�� In Reproduction, Anne Rachelle Howlett

Undergraduate Honors Capstone Projects

The proper formation of gametes is critical for the propagation of species and for the fertility of the individual. The molecular pathways involved in gamete formation remains elusive, therefore, identification of genes involved is an important prerequisite to further our understanding of reproduction. This research will improve infertility treatments and prevention methods in animals and humans. We created mutants that lacked AP-2�� transcription factor after the mouse had entered meiosis and initiated folliculogenesis. The AP-2�� mutants were still fertile and phenotypically similar to normal mice that expressed AP-2�� transcription factor. We also produced mutants that lacked AP-2�� transcription factor during …

Ua66/5/2 Newsletter, Wku Department Of Agriculture

WKU Archives Records

Newsletter regarding programs, events, students and alumni of the WKU Agriculture department.

2006 Annual Report, Various Authors

Annual Reports

No abstract provided.

Characterization Of Dairy Milk House Waste Water In Kentucky, Anshu Singh, Czarena L. Crofcheck, Gail M. Brion

Biosystems and Agricultural Engineering Faculty Publications

This study focuses on characterization of milk house waste water from eight different farms in Kentucky. The farms were separated into three groups based on the number of cows: small (20-30), medium (30-60), and large (over 60 cows). Samples were collected once a month from four farms and twice a month from the remainder. Samples were analyzed for chemical, biochemical, and microbiological characteristics. Results indicated a large and significant variation in the chemical and microbiological characteristics between the farms. Farm size had a significant effect on the nutrient content of the waste water. Though samples exhibited seasonal variation, there was …

Returns To R&D Investment Of Dafwa: Benefit Cost Analysis, 2005-2006, Nazrul Islam

Bulletins 4000 -

The primary outcome or objective of the projects that are assessed, is to increase the market competitiveness and profitability of agri-industry.

Effects Of Refrigeration And Calcium On Whey Protein Aggregation, M. R. Costa, Guillaume Brisson, M. L. Gigante, Phillip S. Tong, Rafael Jiménez-Flores

Dairy Science

Abstract of paper presented at the 2007 Joint Annual Meeting of the American Dairy Science Association & the American Society of Animal Science.

Lipid Binding Characterization Of Lactic Acid Bacteria In Dairy Products, D. Bachiero, Salvador Uson, Rafael Jiménez-Flores

Dairy Science

Abstract of paper presented at the 2007 Joint Annual Meeting of the American Dairy Science Association & the American Society of Animal Science.

Flavor Partition And Fat Reduction In Cheese By Supercritical Fluid Extraction: Processing Variables, Jessica L. Yee, Hany Khalil, Rafael Jiménez-Flores

Dairy Science

Developing low-fat cheese with flavor to match that of full-fat cheese has been a challenge in the dairy industry. The objective of this investigation was to develop lower fat Cheddar and Parmesan grated cheese using supercritical fluid extraction (SFE) and characterize its flavor profile comparative to a full-fat product. Specifically, enabling flavor compound partition between the matrices of cheese and extracted lipids. Carbon dioxide (CO₂) was the supercritical fluid for fat extraction. Extraction took place in a 500 mL SFE vessel using 100 g of grated cheese. Fractional factorial design was utilized to investigate two levels of treatment …

Dairy Digest 2007, South Dakota State University Dairy Club

Dairy Digest

No abstract provided.

The Jackrabbit Dairy Review, Vikram Mistry

The Jackrabbit Dairy & Food Review

No abstract provided.

Caspase 3-Dependent Cell Death Of Neurons Contributes To The Pathogenesis Of West Nile Virus Encephalitis, M. A. Samuel, John D. Morrey, M. S. Diamond

John D. Morrey

West Nile virus (WNV) is a neurotropic, arthropod-borne flavivirus that has become a significant global cause of viral encephalitis. To examine the mechanisms of WNV-induced neuronal death and the importance of apoptosis in pathogenesis, we evaluated the role of a key apoptotic regulator, caspase 3. WNV infection induced caspase 3 activation and apoptosis in the brains of wild-type mice. Notably, congenic caspase 3–/– mice were more resistant to lethal WNV infection, although there were no significant differences in the tissue viral burdens or the kinetics of viral spread. Instead, decreased neuronal death was observed in the cerebral cortices, brain stems, …

Defining Limits Of Treatment With Humanized Neutralizing Monoclonal Antibody For West Nile Virus Neurological Infection In A Hamster Model, John D. Morrey, V. Siddharthan, Aaron L. Olsen, H. Wang, Justin G. Julander, Jeffery O. Hall, H. Li, J. L. Nordstrom, S. Koenig, S. Johnson, M. S. Diamond

John D. Morrey

A potent anti-West Nile virus (anti-WNV)-neutralizing humanized monoclonal antibody, hE16, was previously shown to improve the survival of WNV-infected hamsters when it was administered intraperitoneally (i.p.), even after the virus had infected neurons in the brain. In this study, we evaluated the therapeutic limit of hE16 for the treatment of WNV infection in hamsters by comparing single-dose peripheral (i.p.) therapy with direct administration into the pons through a convection-enhanced delivery (CED) system. At day 5 after infection, treatments with hE16 by the peripheral and the CED routes were equally effective at reducing morbidity and mortality. In contrast, at day 6 …

Efficacy Of Orally Administered T-705 On Lethal Avian Influenza A (H5n1) Virus Infections In Mice, R. W. Sidwell, Dale L. Barnard, C. W. Day, Donald F. Smee, K. W. Bailey, M. H. Wong, John D. Morrey, Y. Furuta

John D. Morrey

T-705 (6-fluoro-3-hydroxy-2-pyrazinecarboxamide) was inhibitory to four strains of avian H5N1 influenza virus in MDCK cells, with the 90% effective concentrations ranging from 1.3 to 7.7 µM, as determined by a virus yield reduction assay. The efficacy was less than that exerted by oseltamivir carboxylate or zanamivir but was greater than that exerted by ribavirin. Experiments with mice lethally infected with influenza A/Duck/MN/1525/81 (H5N1) virus showed that T-705 administered per os once, twice, or four times daily for 5 days beginning 1 h after virus exposure was highly inhibitory to the infection. Dosages from 30 to 300 mg/kg of body weight/day …

Neutralizing Viruses In Suspensions By Copper Oxide-Based Filters, G. Borkow, R. W. Sidwell, Donald F. Smee, Dale L. Barnard, John D. Morrey, H. H. Lara-Villegas, Y. Shemer-Avni, J. Gabbay

John D. Morrey

We report the capacity of copper oxide-containing filters to reduce infectious titers of a panel of viruses spiked into culture media. Enveloped, nonenveloped, RNA, and DNA viruses were affected, suggesting the possibility of using copper oxide-containing devices to deactivate a wide spectrum of infectious viruses found in filterable suspensions.

Use Of Exogenous Fibrolytic Enzymes To Enhance In Vitro Fermentation Of Alfalfa Hay And Corn Silage, Jong-Su Eun, K. A. Beauchemin, H. Schulze

Animal, Dairy, and Veterinary Science Faculty Publications

Two in vitro experiments were performed to identify promising exogenous fibrolytic enzyme products (EFE) and optimum dose rates (DR) for improving the degradation of alfalfa hay and corn silage. The relationship between enzymatic activity and fermentation responses was examined to identify optimum formulations. In experiment 1, 5 EFE containing mainly endoglucanase and xylanase activities, with different ratios between the 2 activities, were assessed at a DR of 0.7, 1.4, and 2.1 mg/g of DM forage. Milled alfalfa hay or corn silage was incubated in an in vitro batch culture with buffer, ruminal fluid, and EFE. Gas production (GP) was measured …

Enhancing In Vitro Degradation Of Alfalfa Hay And Corn Silage Using Feed Enzymes, Jong-Su Eun, K. A. Beauchemin

Animal, Dairy, and Veterinary Science Faculty Publications

A series of in vitro fermentation experiments was performed to assess the effects of 4 feed enzyme products (FE) that varied in enzymatic activities on the degradation of alfalfa hay and corn silage. The FE contained a range of endoglucanase, exoglucanase, xylanase, and protease activities, and a range of dose rates (DR) was used. The objective of the study was to identify effective formulations and optimum DR, and to establish if combining FE would further improve fiber degradation. For alfalfa hay, quadratic increases in gas production and degradation of dry matter (DM) and fiber were observed for all FE, with …

Producción Y Calidad De Leche En Vacas Holstein En Dos Arreglos Silvopastoriles De Acacia Decurrens Y Alnus Acuminata Asociadas Con Pasto Kikuyo, Pennisetum Clandestinum, Eugenio Gualdron Calderón, Cesar Eduardo Padilla Charry

Zootecnia

La investigación se llevó a cabo en la finca Villa Nataly, Vereda Olarte, localidad de Usme. Según clasificación Holdridge es Bosque muy húmedo montano bajo; precipitaciones de 800 a 1000 mm, temperaturas entre 6 y 12 C°. Los suelos predominantes en la finca son de textura franca, buen drenaje y notable susceptibilidad a la erosión. Los tratamientos evaluados fueron dos arreglos silvopastoriles uno conformado por Alnus acuminata asociado con Pennisetum clandestinum denominado sistema silvopastoril de sombra (SSPS), y otro arreglo conformado por Acacia decurrens + A. acuminata asociados con P. clandestinum, denominado sistema silvopastoril de sombra y ramoneo (SSPSR), comparados …

Timed Artificial Insemination Conception Rates In Response To A Progesterone Insert In Lactating Dairy Cows (2007), C.A Martel, B.S. Buttery, M.G. Burns, W.E. Brown

Kansas Agricultural Experiment Station Research Reports

Our objective was to determine the effectiveness of exogenous progesterone in the form of an intravaginal insert (controlled internal drug release, CIDR) in conjunction with an ovulation synchronization protocol in lactating dairy cows. Cows received a Presynch protocol (two injections of prostaglandin F2α[PGF2α] 14 days apart) beginning 30 and 36 days in milk, respectively, in two herds. Cows were inseminated after the second Presynch injection when estrus was detected. Remaining cows were treated with the Ovsynch protocol, and alternate cows were assigned randomly to receive a progesterone insert(CIDR). Blood was collected, and body condition scores (BCS) were assigned to treated …

Impact Of Slope And Pipe Diameter On Flush Plume Design (2007), Joseph P. Harner, John F. Smith, Michael J. Brouk

Kansas Agricultural Experiment Station Research Reports

Manning's equation provides a method to evaluate the flow characteristics of a flush plume system used to move a diluted, sandladen manure stream from a freestall building to sand or solid separation equipment. Evaluation of a 16, 18, and 24-inch plume showed pipe slope is critical in maintaining a 5 feet per second water velocity through the pipe. A 24 inch or larger plume placed on a 0.5% slope is able to obtain water velocity of 5 feet per second if the pump capacity exceeds 3,600 gpm. The flow velocity never reached or exceeded 5 feet per second in a …

Intracellular Lipids In Bos Indicus And Box Taurus Oocytes, Casey Bryant Ballard

LSU Master's Theses

It has been proposed that Bos indicus when compared with Bos taurus embryos are more sensitive to cryopreservation procedures due to higher intracellular lipid content of the embryos. With cyropreservation of Bos indicus embryos increasing for export, it is essential to improve the viability of these cryopreserved embryos. In Experiment I, M-II oocytes from mice, pigs and cows were subjected to two different procedures to evaluate lipid content. The lipid content present in pig oocytes was significantly (P<0.05) higher when compared with mouse and cow oocytes. Pig oocytes had a lower settling point in the sucrose buoyant density gradient and higher Nile Red units, which indicated higher lipids present in the oocytes. In Experiment II, it was determined that M-II oocytes aspirated from superstimulated Brahman donors were significantly higher (P<0.05) in lipid content compared with English breed donors. The lipid content present in Brahman oocytes was higher when compared with English breeds when tested with a sucrose buoyant density gradient and Nile Red staining. In addition, donor females with higher body condition scores were significantly (P<0.05) higher in oocyte lipid content when compared with donor females of lower body condition scores. Furthermore, animal body condition scores, cholesterol and triglyceride levels prior to oocyte aspiration may be indicators of intracellular lipids in the oocytes and embryos. To our knowledge, this is the first report that evaluated intracellular lipid content of M-II oocytes of different beef breed types using a sucrose buoyant density gradient and Nile Red staining.

Detection Of Noncyling Cows By Heatmount Detectors And Ultrasound Before Treatment With Progesterone (2007), D.E. Tenhouse, R.L. Krisher, G.C. Lamb, C.R. Dahlen, D.J. Brusveen, M. Burkhart, R.S. Youngquist, H.A. Garverick, Jeffrey S. Stevenson

Kansas Agricultural Experiment Station Research Reports

Our objective was to determine accuracy of identifying anovulatory lactating dairy cows before the application of a timed AI protocol [with or without progesterone supplementation via a controlled internal drug release (CIDR) insert and 2 different timings of AI] by using heatmount detectors and a single ovarian ultrasound examination. At 6 Midwest locations, 1,072 cows were enrolled in a Presynch protocol (2 injections of prostaglandin F2α(PGF2α) 14 days apart) with the second injection administered 14 days before initiating the Ovsynch protocol (injection of gonadotropin releasing hormone (GnRH) 7 days before and 48 hours after PGF2αinjection, with timed AI at 0 …

Nutritional Strategies For A Healthy Transition To Lactation: An Update (2007), Barry J. Bradford

Kansas Agricultural Experiment Station Research Reports

Reducing cull rates and improving fertility in early lactation has a dramatic effect on the profitability of many dairies. Continuing research on transition cow nutrition has led to the development of an array of nutritional strategies to prevent disorders during the transition period. It is important, however, to realize that some of these strategies have similar modes of action, and as such, their effects are not likely to be additive. Producers should work with their nutritionist and veterinarian to identify the most prevalent transition problems in the herd and review options for preventing those disorders.; Dairy Day, 2007, Kansas State …