Dairy Science Commons^™

Rumen-Protected Methionine Supplementation Improves Lactation, Oxidative Status, And Immune Response During A Subclinical Mastitis Challenge In Lactating Dairy Cows., Anita Paz Lopez

Electronic Theses and Dissertations

Mastitis is a significant problem in the dairy industry, posing numerous challenges for dairy farmers worldwide. Mastitis refers to the inflammation of the mammary gland, primarily caused by bacterial infections. This condition affects the health and well-being of dairy cows and has severe economic implications for farmers and overall dairy production. The prevalence of mastitis substantially threatens milk quality, productivity, and profitability, making it a top concern in the dairy industry. Preventing mastitis in cows is crucial for maintaining the herd's health and ensuring highquality milk production. Nutritional strategies have recently gained greater attention due to a direct impact on …

Comparison Of Bovine Mammary Involution And Intramammary Infections Following Intramammary Treatment With Casein Hydrolysate And Other Conventional Treatments At Dry-Off, Justine E. Britten, Kerry A. Rood, David J. Wilson

Animal, Dairy, and Veterinary Science Student Research

Alternatives to routine antibiotic treatment of dairy cattle during the dry period before their next calving are of interest. This was a preliminary study of whether intramammary infusion of casein hydrolysate, administered alone or combined with standard dry treatment, accelerated the rate of mammary involution early in the dry period. Four treatments were studied in a split udder design. One udder half was assigned a treatment, and the contralateral half was administered dry cow treatment + internal teat sealant as a control. Treatments were casein hydrolysate, casein hydrolysate + dry cow treatment, casein hydrolysate + teat sealant and casein hydrolysate …

Profile Of Antimicrobial Susceptibility From Cattles’S Milk Isolates Suffering From Mastitis In District Lahore, Muhammad Imran, Iqra Rehman, Abdul Qayyum Khan Sulehria, Yasser Mustafa Butt, Abdul Majid Khan, Azizah Ziauddin

Journal of Bioresource Management

Mastitis is highly prevalent infection in cattle causing cost-effective loss in dairy milk production. Escherichia coli is the most frequently isolated bacteria causing mastitis worldwide. The current study was performed to investigate the mastitis prevalence and effect of different antibiotics against pathogens causing it. In sum, 216 milk samples were collected randomly including 108 each from both cows and buffaloes. These samples were subjected to Surf Field Mastitis Test for prevalence of mastitis that found 18.50% and 23.14% in buffaloes and cows respectively. Mastitis was more prevalent both in buffaloes and cows at the age of 9-10 years that was …

Evaluation Of Casein Hydrolysate As An Alternative Dry-Off Treatment And Milk Quality Management Tool In Dairy Cows, Justine Elena Britten

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Mastitis, an infection of the mammary gland, is the most common and expensive animal health problem for the dairy industry and affects every dairy farm to some degree. This disease complex is painful for dairy cows, increases the on-farm use of antibiotics, presents a threat to milk quality and is a waste of time, money and milk production. Each year, the dairy industry loses as much as a billion dollars to mastitis.

Many cows will experience mastitis at least once during a lactation cycle and some animals will develop recurring mastitis episodes in a single mammary quarter. These mastitic quarters …

The Role Of T-Cell Lymphoma Invasion And Metastasis 2 (Tiam2) In The Barrier Function Of Mammary Epithelial Cells Following Streptococcus Uberis Inoculation, Catherine E. Donnell

Chancellor’s Honors Program Projects

No abstract provided.

Diseño De Un Protocolo De Ordeño Mecánico En El Hato De La Hacienda La Alcancía En El Municipio De Sopó, Cundinamarca, Alejandro Suárez

Zootecnia

El presente estudio se realizó en la hacienda La Alcancía, ubicada en el municipio de Sopó, Cundinamarca, para lo cual, se tomaron 20 vacas de segundo y tercer parto como unidades experimentales, las cuales fueron distribuidas aleatoriamente en dos grupos; 10 animales como grupo control y 10 como grupo experimental. Los animales del grupo experimental, entraron a ser tratados por el nuevo protocolo de ordeño; este diseño, consiste básicamente en la reducción de los tiempos de pre-ordeño, ordeño y post ordeño a un máximo de seis minutos por animal; disminución del estrés y adrenalina, evitando la inhibición de oxitocina para …

Beta-Hydroxybutyrate Alters The Mrna Cytokine Profile From Mouse Macrophages Challenged With Streptococcus Uberis, T. H. Swartz, L. K. Mamedova, B. J. Bradford

Kansas Agricultural Experiment Station Research Reports

The objective of this study was to determine if β-hydroxybutyrate (BHB) altered inflammatory responses in macrophages challenged with a common mastitis pathogen,Streptococcus uberis. Mouse macrophages (RAW 264.7 line) were cultured either in the presence or absence of BHB for 24 h, and then challenged or not withS. uberis. Relative transcript abundance of cell membrane receptors (TLR2 and GPR109a), cytokines (IL-1β, IL-10, TNFα, and TGFβ), and chemokines (CXCL2 and CCL5) were determined using quantitative real-time polymerase chain reaction (qPCR) and normalized against the geometric mean of HPRT and B2M.Streptococcus uberisactivated the macrophages, noted by greater …

The Effects Of Housing On Dairy Cow Comfort, Immune Function, Stress, Productivity, And Milk Quality, Matthew Richard Borchers

Theses and Dissertations--Animal and Food Sciences

Mastitis and milk quality affect every dairy farmer across the globe. Sand bedded freestalls are the industry standard for cow comfort, welfare, and the control of environmental mastitis. Compost bedded packs may be a viable alternative to the sand bedded freestall.

Compost bedded packs are maintained at a consistent level of moisture, nutrients, and aeration to favor compost microorganisms. Greater bacteria counts in bedding have traditionally been associated with increased mastitis rates and mastitis pathogens can be found in the pack and on the teats of cattle housed in even well managed compost bedded pack barns. In spite of this, …

Identifying Genome Associations With Unique Mastitis Phenotypes In Response To Intramammary Streptococcus Uberis Challenge, Lydia Jean Siebert

Doctoral Dissertations

Mastitis, the inflammation of the mammary gland, dramatically decreases dairy industry revenues and milk quality, making improved control and prevention methods a goal of the industry. Prior research has observed variation in response to experimental challenge with regards to inflammation indicators, S. uberis concentrations, and the need for antibiotics. To determine possible causes of the observed variation following experimental S. uberis intramammary challenge, we performed genome association analyses (N = 34 – 36 Holstein dairy cows), a linkage analysis, and S. uberis milk inoculation (N = 21 Holstein dairy cows) trials. Association analyses were done using Illumina’s BovineSNP50 BeadChip and …

Precision Dairy Farming Technology Solutions For Detecting Dairy Cow Disease To Improve Dairy Cow Well-Being, Amanda Stone

Theses and Dissertations--Animal and Food Sciences

Dairy cow health is multifactorial and complex. High producing dairy cows have been described as metabolic athletes, but metabolic and infectious diseases around calving affect many cows. These diseases have drastic negative effects on dairy cow well-being, milk production, and dairy farm economics. Early disease detection could potentially improve disease management, treatment, and future prevention techniques. The first objective of this research was to evaluate the use of activity, lying behavior, reticulorumen temperature, and rumination time determined by precision dairy farming technologies to detect transition cow diseases including hypocalcemia, ketosis, and metritis. The second objective was to evaluate the ability …

Identifying Genetic Factors In Streptococcus Uberis That Enable Evasion Of The Host Immune Response, Alexis Christine Burnham

Chancellor’s Honors Program Projects

No abstract provided.

Efecto Del Sistema De Ordeño En La Calidad De La Leche De Los Productores De La Cooperativa Colega, Juliana Arguello Benítez

Zootecnia

Este trabajo se desarrolló con el apoyo de los asociados de la Cooperativa Colega que se encuentra ubicada en Guatavita, Cundinamarca, cuenta con 21 productores vinculados, un inventario de 421 animales de los cuales 200 se encuentran en producción, con un promedio de producción de 19.4 litros / animal / día, manejan distintas razas (Holstein, Ayrshire, Rojo sueco, Normando, Jersey y sus cruces), se pretende establecer la relación entre el sistema de ordeño implementado y la calidad composicional e higiénica (recuento de células somáticas) en los sistemas productivos de la Cooperativa Colega. El objetivo principal de este estudio fue relacionar …

Genetic Resistance To Staphylococcus Aureus Mastitis Associated With Bovine Cxcr1, Olivia Jean Crisp

Chancellor’s Honors Program Projects

No abstract provided.

Bovine Mammary Interleukin-8 Receptor Expression And Genetic Association With Streptococcus Uberis Based Mastitis., Lydia Jean Siebert

Masters Theses

Mastitis is an economically distressing disease in the dairy industry. Bacterial pathogens enter the gland and encounter cell types that release immune mediators including interleukin (IL)-8. IL-8 has two membrane bound receptors: CXCR1 and CXCR2. CXCR1 and CXCR2 are expressed on neutrophils and other cell types in other species but their expression is unknown in the bovine mammary gland. To test this, mammary tissue samples from six Holstein dairy cows were subject to dual immunofluorescence with bovine specific CXCR1 and CXCR2 antibodies and cell type markers. CXCR1 was expressed on alveolar epithelial cells, fibroblasts, and leukocytes. CXCR2 expression was identified …

Testing The Effect Of Grapefruit Seed Extract (Gfse) On The Mastitsis-Causing Alga, Prototheca, Kendra Janelle Macdonald

Honors College

Mastitis is an inflammation of the mammary gland that costs the United States dairy industry between $1.7 and $2 billion annually [9]. Approximately 97% of mastitis infections are caused by bacteria, which can be treated with antibiotics [2]. However, mastitis infections can be caused by the alga, Prototheca. Specifically, it is P. zopfii (genotype 2) and P. blaschkeae that have been found in cows with mastitis [7]. There are currently no approved treatments for protothecal mastitis. The following experiments a tested the effects of grapefruit seed extract (GFSE) on P. zopfii (genotype 2) using spectrophotometry and plating techniques. GFSE was …

Milking Protocols At The Cal Poly Dairy Farm, Kevin Mohan

Dairy Science

Milking parlors are the center of a dairy operation and directly responsible for its profits, yet dairy training is frequently neglected and often lackluster. The Cal Poly dairy farm, despite having a milker pool that is comparatively inexperienced with farm labor has no written manual for training of milkers. This results in a workplace that in the event of an accident or point of confusion leaves workers nowhere to turn to for clarification save contacting a supervisor directly. It also may also result in a lack of established guidelines, and a frequent need for clarification due to misunderstood expectations and …

Management And Technology Solutions For Improving Milk Quality, Amanda E. Sterrett

Theses and Dissertations--Animal and Food Sciences

Mastitis is one of the most common and expensive dairy cattle diseases. Mastitis prevention and management are key factors in herd health and improved milk quality. One objective of this research was to evaluate management solutions to maintain a low somatic cell count, based on survey responses from Kentucky dairy producers. Because hyperkeratosis may increase mastitis incidence, another objective of this research was to examine changes in teat end hyperkeratosis in a herd transitioning from a standard pulsation milking system to an individual quarter pulsation milking system. The last objective of this research was to evaluate technologies that monitored rumination …

The Efficacy Of Antibiotic Residue Screening Tests For The Detection Of Natural Antimicrobials In Milk, Stacey L. O'Donnell

Master's Theses

No abstract provided.

Identifying Mechanisms Associated With Innate Immunity In Cows Genetically Susceptible To Mastitis, Alexandra Alida Elliott

Doctoral Dissertations

Mastitis, or mammary gland inflammation, causes the greatest loss in profit for dairy producers. Mastitis susceptibility differs among cows due to environmental, physiological, and genetic factors. Prior research identified a genetic marker in a chemokine receptor, CXCR1, associated with mastitis susceptibility and decreased neutrophil migration. Current research seeks to identify reasons behind mastitis susceptibility by validating this model through in vivo challenge with Streptococcus uberis and studying specific mechanisms causing impaired neutrophil migration. Holstein cows with GG (n=19), GC (n=28), and CC (n=20) genotypes at CXCR1+777 were challenged intramammarily with S. uberis strain UT888. After challenge 68% of quarters from …

A Comparison Of Pyronin Y-Methyl Green Stain And Methylene Blue Stain For Somatic Cell Count In Sheep Milk, Emily Mirek, Stacey O’Donnell

Senior Honors Projects

Somatic cell count is a key method used to evaluate the quality of milk in today’s growing dairy sheep industry. Somatic cells are body cells, primarily the white blood cells, found in a milk sample. If an infection such as mastitis is present, the number of somatic cells in the milk increases (Gonzalo, et al. 1992). Producers routinely perform somatic cell counts on cow and goat milk. For dairy cattle in the United States, the somatic cell count can not exceed 750,000 cells per milliliter, for commercial milk. For dairy goats the accepted limit is 1,000,000 cells per mL of …

Efficacy Of Prepartum Intramammary Lactating Cow Treatment In Dairy Heifers, Christopher B. Norman

LSU Master's Theses

Mastitis in prepartum dairy heifers has been recognized as a significant economic problem for the dairy industry. Intramammary infusion of cephapirin sodium was completed in 20 treatment animals and the results were compared to 25 non-treated control animals. Bacteriological data from the quarter milk samples were used to determine and compare initial infection rates and cure rates following calving between treatment and control groups. Comparisons were also made between groups for differences in reproductive performance, milk yield, somatic cell count and milk ketone concentration. Milk yield did not differ between groups for either DHIA monthly average kg/day, 305 day actual …

Tail Docking Dairy Cattle: Effects On Cow Cleanliness And Udder Health, Cassandra B. Tucker, David Fraser, Daniel M. Weary

Farm Animal Husbandry Collection

To determine whether tail docking would influence cow cleanliness and udder health in a free-stall system, we monitored milking cows after half the animals in a herd were docked. A sample of 223 docked and 190 undocked cows (reducing to 169 and 105 over the study as cows were dried off) were monitored for 8 wk. Cow cleanliness was scored in two areas: along the spine, and the rump adjacent to the tail at 1, 2, 3, 5, and 8 wk after docking. Cleanliness was evaluated by counting squares that were soiled (0 to 14 on a 5- × 17.5-cm …

Effects Of Mastitis On Milk Yield And Composition, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

EVERY DAIRY farmer knows how much he spends on the treatment of cows affected with mastitis, but the actual loss in production is more difficult to estimate.

As part of the current mastitis research programme the Department of Agriculture's Animal and Dairying Divisions surveyed 37 dairy herds with a believed high incidence of mastitis.

Can Mastitis Be Eliminated?, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

Can mastitis be eliminated from a dairy herd? The results of a 12-month continuous study of four commercial herds show that it could be done.

Mastitis In Western Australian Dairy Cattle, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

IN 1964/65, as a first step towards a State-wide mastitis control programme, the Department of Agriculture undertook a survey of mastitis in West Australian dairy herds.

The survey was designed to obtain a clear picture of the incidence and severity of the disease and is the most comprehensive mastitis survey carried out in Australia.

Mastitis : Modified Teat Cups Give Better Back-Flushing, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

Back-flushing of the teat cups has not brought the expected reduction in the spread of mastitis in some dairy herds.

Replacement of the conventional nut-and-tail nipple with a special flushing nipple, or use of a one-piece teat cup liner, will ensure that flushing is efficient.

Animal Husbandry Research At Bramley, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

A progress report of animal husbandry investigations at the Bramley Animal Husbandry Research Station, Margaret River.

BRAMLEY RESEARCH STATION is four miles north of Margaret River.

The average annual rainfall of 45 inches falls mainly in the winter, providing a seven-month growing season which starts in March or April.

The June-July period is excessively wet, causing extensive waterlogging of the soils.

Mastitis : Prevention And Control. 3. Implementing The Mastitis Control Programme, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

FARMERS who have followed the first two articles in this series should by now have established the recommended milking routine and be familiar with it.

Mastitis : Prevention And Control. 2. Principles Of Mastitis Control, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

MASTITIS is a major cause of financial loss in over half our dairy herds.

Losses result from treatment costs, milk discarded, permanent loss of milk production, culling, lowering of solids-not-fat content and loss of butter-fat production.

Mastitis CAN be controlled. This article outlines the basic principles of mastitis control in the milking shed.

Mastitis : Its Prevention And Control. 1. The Udder In Health And Disease, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

This is the first of a series of four articles on mastitis which will appear in consecutive issues of the Journal of Agriculture.

This is an introductory article describing the cow's udder in health and disease.