Dairy Science Commons^™

Temporary Cattle Identification: Benefits Of Marking With Hair Bleach, Melanie Heaton, Reganne K. Briggs, Rebekah Iverson, Brooke Holgate, David Secrist

All Current Publications

Temporary identification is an important management tool for beef producers. Animals are routinely marked following breeding, pregnancy diagnosis, vaccination, treatment, sorting, shipping, and marketing so they can be quickly identified without repeated handling. Effective temporary identification improves labor efficiency, reduces animal stress, and helps ensure management decisions are applied to the correct animals. This fact sheet provides information on temporary cattle marking using hair bleach.

Improving Public Perceptions Of Dairy Production Through On-Farm Educational Tours, Drew Swartz, Justin Wyatt Clawson, Jaydee Gunnell, Elise Jenson, Dylan Waldron, Jenna Dyckman

Outcomes and Impact Quarterly

Utah dairy producers identified public perceptions as a top concern. Utah State University Extension partnered with Cache County organizations to offer dairy farm tours. Participants reported increased knowledge, more favorable perceptions, and stronger intentions to engage with agriculture, supporting experiential education as a means to strengthen understanding of dairy production.

Cross-Reactivity Of 15 Jev Antigen-Directed Rabbit Antisera With Three Clinically Important Jev Serogroup Members: Wnv, Mvev, And Slev, Sang-Im Yun, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Japanese encephalitis virus (JEV), the prototype member of the JEV serogroup within the genus Orthoflavivirus (family Flaviviridae), is closely related to West Nile virus (WNV), Murray Valley encephalitis virus (MVEV), and St. Louis encephalitis virus (SLEV). To characterize antigenic cross-reactivity within this group, we evaluated 15 region-specific rabbit antisera, previously generated against nearly the entire JEV protein-coding region, by immunoblotting whole-cell lysates from BHK-21 cells infected with WNV, MVEV, or SLEV, with JEV included as a reference. Six antisera (α-E^N-term, α-NS2B, α-NS3^N-term, α-NS3^C-term, α-NS5^N-term, and α-NS5^C-term) robustly recognized homologous …

Lameness In Dairy Cattle: A Practical Overview, Drew Swartz, Kalen Taylor, Jacob Hadfield, Justin Wyatt Clawson

All Current Publications

This fact sheet provides an overview of lameness in dairy cattle, including what it is, why it matters, and how to recognize and manage it. Information is geared toward producers, consultants, and educators.

Three Common Hoof Problems In Dairy Cows: What To Look For And How To Prevent Them, Drew Swartz, Kalen Taylor, Jacob Hadfield, Justin Wyatt Clawson

All Current Publications

This fact sheet covers the three most common hoof lesions in dairy cows and explains why they matter and how to prevent them on your farm.

Digital Dermatitis, Sole Ulcers, And White Line Disease In Dairy Cattle: Pathogens, Diagnosis, And Treatment, Drew Swartz, Kalen Taylor, Jacob Hadfield, Justin Wyatt Clawson

All Current Publications

This fact sheet focuses on three common hoof lesions in dairy cattle: digital dermatitis, sole ulcers, and white line disease, and describes how differences in pathogenesis and risk factors influence diagnosis, prevention, and treatment.

Colostrum Management For Dairy Calves: Key Practices For Calf Health And Performance, Drew Swartz, Jacob Hadfield, Kalen Taylor, Maggi Mathews

All Current Publications

This fact sheet provides practical recommendations for dairy producers to improve colostrum management and support calf health and performance.

Colostrum Management For Dairy Calves: Technical Guidelines For Consultant Evaluation And Implementation, Drew Swartz, Jacob Hadfield, Kalen Taylor, Maggi Mathews

All Current Publications

This fact sheet provides a technical overview of colostrum management in dairy calves, including evaluation methods and evidence-based recommendations for consultants, educators, and students.

Colostrum And Rethinking Passive Transfer In Dairy Calves, Drew Swartz, Kalen Taylor, Maggi Mathews

All Current Publications

This fact sheet provides an overview of colostrum and passive transfer in dairy calves, with information applicable to producers, consultants, educators, and students.

Coenzyme Q10 Improves Functional And Structural Parameters Of Dairy Goat Sperm During Cooling And Cryopreservation, Ranadheer Narlagiri, Abdallah M. Shahat, Courtney Henry, Ashvini Pawar, Niki C. Whitley, Iman B. Shaheed, Mahipal Singh, Brou Kouakou, Irina A. Polejaeva, Adel R. Moawad

Animal, Dairy, and Veterinary Science Faculty Publications

Cryopreservation of gametes is crucial for conserving genetic diversity in livestock and endangered species, but the process can significantly impair sperm quality due to oxidative stress. Our aim was to evaluate the impacts of coenzyme Q10 (CoQ10) supplementation on the in vitro quality of cooled and cryopreserved goat semen. Semen samples collected from six mature Saanen bucks were pooled then diluted with AndroMed^® semen extender to a final concentration of 800 × 10⁶ sperm/mL. Diluted semen was supplemented with 0, 1, 2, 5, 10, and 20 µM CoQ10. Extended semen was either cooled at 4 °C for 72 …

A Bovine Cell Line Resistant To Japanese Encephalitis Virus Entry But Permissive To Post-Entry Replication, Sang-Im Yun, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Japanese encephalitis virus (JEV) is a mosquito-borne zoonotic orthoflavivirus that poses a significant global health threat. It causes severe neuroinflammatory disease in humans and reproductive failure in swine. Because of the broad host range and cell tropism of JEV, identifying animal cell lines resistant to infection has been a persistent challenge. In this study, we demonstrate that Madin–Darby bovine kidney (MDBK) cells are resistant to JEV infection yet remain fully permissive to viral replication when transfected with viral genomic RNA. Using immunoblotting, immunofluorescence, and flow cytometry, we show that MDBK cells, unlike the highly susceptible baby hamster kidney (BHK-21) cells …

Effect Of The Ahr Inhibitor Ch223191 As An Adjunct Treatment For Mammarenavirus Infections, Miguel Angel Pelaez, Jonna B. Westover, Dionna Scharton, Cybele Carina García, Brian B. Gowen

Animal, Dairy, and Veterinary Science Faculty Publications

The family Arenaviridae encompasses zoonotic, rodent-borne pathogens (e.g., Lassa, Machupo, and Junín viruses) that cause severe viral hemorrhagic fevers with high case fatality rates. The current therapeutic landscape is severely limited, underscoring the urgent need for novel antiviral strategies. A promising approach involves combining directly acting antivirals with host-targeted antivirals. A compelling host-targeted antiviral target is the aryl hydrocarbon receptor (AHR). This ubiquitous ligand-activated transcription factor is a recognized pro-viral host factor across multiple viral families. Building on prior work with Junín and Tacaribe viruses, we investigated whether the AHR inhibitor CH223191 could enhance the virus-directed antiviral activity of favipiravir …

Homologous And Heterologous Vaccination Regimens With Mrna And Rvsv Platforms Induce Potent Immune Responses Against Sftsv Glycoprotein, Tomaz B. Manzoni, Jonna B. Westover, Kendall A. Lundgreen, Philip D. Hicks, Raegan J. Petch, Jordan T. Ort, Drew Weissman, Steven H. Y. Fan, Scott E. Hensley, Norbert Pardi, Brian B. Gowen, Paul Bates

Animal, Dairy, and Veterinary Science Faculty Publications

Background: Severe fever with thrombocytopenia syndrome virus (SFTSV) is a highly pathogenic bunyavirus with a high case-fatality ratio for which there is no approved vaccine. Studies have assessed different vaccine technologies. However, few studies have yet assessed the immunogenicity of heterologous prime-boost regimens. Methods: Here, we compare a lipid nanoparticle (LNP)-encapsulated nucleoside-modified mRNA-based vaccine encoding the SFTSV glycoproteins, Gn and Gc, to our recently described recombinant VSV SFTSV (rVSV-SFTSV) vaccine in single dose, homologous, and heterologous prime-boost regimens in mice. Results: We show that all regimens protect from pathogenic SFTSV challenge and elicit strong long-lasting antibody responses. Furthermore, strong cellular …

Tube-Feeding A Calf, Cheyenne Reid, Reganne K. Briggs, Melanie Heaton, Rebekah Esplin, David Secrist

All Current Publications

Producers may need to tube-feed a newborn calf to provide milk if calves are unable to nurse or suck from the nipple bottle or when calves are sick and need electrolytes. This fact sheet outlines the materials required and the steps to successfully tube-feed a calf.

Warming A Calf, Cheyenne Reid, Reganne K. Briggs, Melanie Heaton, Rebekah Esplin, David Secrist

All Current Publications

Calves born in the winter or spring are often exposed to harsh weather and environmental conditions. After birth, calves may become chilled due to cold temperatures or not being dried off promptly. A chilled calf may be experiencing hypothermia. This fact sheet explains the different types of hypothermia and methods for warming a calf until it can be safely returned outside.

Evaluating A Proprietary Tannin-Blend Product As An Alternative To Monensin And Tylosin Phosphate In Feedlot Cattle Diets, Luana D. Felizari, Luke K. Fuerniss, Jonathon L. Beckett, David S. Secrist, Guy D. Hufstedler, Bradley J. Johnson

Animal, Dairy, and Veterinary Science Faculty Publications

This study evaluates the effects of a proprietary tannin blend (BX), supplemented with or without sodium monensin (MON), on beef cattle performance, carcass traits, and health. Steers (n = 2986; initial shrunk body weight (SBW) 254 ± 9.2 kg) were allocated into 48 pens (61–62 steers/pen; 12 pens/treatment) fed for 230 d. Treatments included (1) no feed additives (CTL); (2) BX (7.95 g/animal daily); (3) MON + Tylosin phosphate (TYL; 437.52 mg MON/animal daily + 80 mg TYL/animal daily); or (4) MON+BX (437.52 mg MON/animal daily + 7.95 g BX/animal daily). Data were analyzed in R 4.2.1 using a …

Genomic-Transcriptomic Analysis Identifies The Syrian Hamster As A Superior Animal Model For Human Diseases, Chuchu Wang, Zhenguo Cheng, Jinxin Miao, Xia Xue, Yunshu Dong, Li Zhao, Haoran Guo, Jianyao Wang, Zhizhong Wang, Shuangshuang Lu, Guangming Fang, Ying Peng, Yafei Zhai, Zhongxian Zhang, Dongling Gao, Zhimin Wang, Pengju Wang, Lirong Zhang, Louisa S. Chard Dunmall, Jun Wang, Wenxue Tang, Xiaowei Li, Zhongren Ding, Xiaoyan Zhao, Ling Li, Nicholas R. Lemoine, Zhongde Wang, Daniel Tonge, Wenjie Tan, Jianzeng Dong, Yaohe Wang

Animal, Dairy, and Veterinary Science Faculty Publications

Background The Syrian hamster (Mesocricetus auratus) has shown promise as a human diseases model, recapitulating features of different human diseases including COVID-19. However, the landscape of its genome and transcriptome has not been systematically dissected, restricting its potential applications.

Results Here we provide a complete analysis of the genome and transcriptome of the Syrian hamster and found that its lineage diverged from that of the Chinese hamster (Cricetulus griseus) around 29.4 million years ago. 21,387 protein-coding genes were identified, with 90.03% of the 2.56G base pair sequence being anchored to 22 chromosomes. Further comparison of the …

Species Differences In The Biotransformation Of Aflatoxin B1: Primary Determinants Of Relative Carcinogenic Potency In Different Animal Species, David L. Eaton, David E. Williams, Roger A. Coulombe

Animal, Dairy, and Veterinary Science Faculty Publications

It has been known since the early days of the discovery of aflatoxin B1 (AFB1) that there were large species differences in susceptibility to AFB1. It was also evident early on that AFB1 itself was not toxic but required bioactivation to a reactive form. Over the past 60 years there have been thousands of studies to delineate the role of ~10 specific biotransformation pathways of AFB1, both phase I (oxidation, reduction) and phase II (hydrolysis, conjugation, secondary oxidations, and reductions of phase I metabolites). This review provides a historical context and substantive analysis of each of these pathways as contributors …

Safety, Immunogenicity, And Efficacy Of A Recombinant Vesicular Stomatitis Virus Vectored Vaccine Against Severe Fever With Thrombocytopenia Syndrome Virus And Heartland Bandavirus, Philip Hicks, Tomaz B. Manzoni, Jonna B. Westover, Raegan J. Petch, Brianne Roper, Brian B. Gowen, Paul Bates

Animal, Dairy, and Veterinary Science Faculty Publications

Background: Severe fever with thrombocytopenia syndrome virus (STSV) is a recently emerged tickborne virus in east Asia with over 18,000 confirmed cases. With a high case fatality ratio, SFTSV has been designated a high priority pathogen by the WHO and the NIAID. Despite this, there are currently no approved therapies or vaccines to treat or prevent SFTS. Vesicular stomatitis virus (VSV) represents an FDA-approved vaccine platform that has been considered for numerous viruses due to its low sero-prevalence in humans, ease in genetic manipulation, and promiscuity in incorporating foreign glycoproteins into its virions. Methods: In this study, we developed a …

Differential Hepatic Expression Of Mirna In Response To Aflatoxin B1 Challenge In Domestic And Wild Turkeys, Kade Jorud, Kristelle M. Mendoza, Thomas Kono, Roger A. Coulombe, Kent M. Reed

Animal, Dairy, and Veterinary Science Faculty Publications

Aflatoxin B1 (AFB₁) is a major foodborne mycotoxin that poses a significant economic risk to poultry due to a greater degree of susceptibility compared to other agricultural species. Domesticated turkeys (Meleagris gallopavo) are especially sensitive to AFB₁; however, wild turkeys (M. g. silvestris) are more resistant. A lack of functional isoforms of hepatic glutathione S-transferases (GSTs), an enzyme that plays a role in the detoxification of aflatoxin, is suspected as the reason for the increased sensitivity. Previous studies comparing the gene expression of domesticated and wild turkeys exposed to AFB₁ identified …

Dietary Milk Phospholipids Increase Body Fat And Modulate Gut Permeability, Systemic Inflammation, And Lipid Metabolism In Mice, Albert Lihong Zhou, Robert E. Ward

Nutrition, Dietetics, and Food Sciences Faculty Publications

The study aimed at how dietary milk polar lipids affect gut permeability, systemic inflammation, and lipid metabolism during diet-induced obesity (DIO). C57BL/6J mice (n = 6x3) were fed diets with 34% fat as energy for 15 weeks: (1) modified AIN-93G diet (CO); (2) CO with milk gangliosides (GG); (3) CO with milk phospholipids (MPL). Gut permeability was assessed by FITC-dextran and sugar absorption tests. Intestinal tight junction proteins were evaluated by Western blot. Plasma cytokines were measured by immunoassay. Body composition was assessed by magnetic resonance imaging. Tissue lipid profiles were obtained by thin layer chromatography. Hepatic expression of genes …

Effects Of Premortem Stress On Protein Expression, Steak Color, Oxidation, And Myofibrillar Fragmentation Index In The Longissimus Lumborum, Reganne K. Briggs, Jerrad F. Legako, Paul R. Broadway, Jeff A. Carroll, Nicole C. Burdick Sanchez, Nikole E. Ineck, Zachary K. Smith, Ranjith Ramanathan, Kara J. Thornton

Animal, Dairy, and Veterinary Science Faculty Publications

No abstract provided.

Expression And Secretion Of Sparc, Fgf-21 And Dcn In Bovine Muscle Cells: Effects Of Age And Differentiation, Katie A. Shira, Kara J. Thornton, Brenda M. Murdoch, Gabrielle M. Becker, Gwinyai E. Chibisa, Gordon K. Murdoch

Animal, Dairy, and Veterinary Science Faculty Publications

Skeletal muscle growth is an economically important trait in the cattle industry. Secreted muscle-derived proteins, referred to as myokines, have important roles in regulating the growth, metabolism, and health of skeletal muscle in human and biomedical research models. Accumulating evidence supports the importance of myokines in skeletal muscle and whole-body health, though little is known about the potential presence and functional significance of these proteins in cattle. This study evaluates and confirms that secreted proteins acidic and rich in cysteine (SPARC), fibroblast growth factor 21 (FGF-21), myostatin (MSTN), and decorin (DCN) are …

Cytokine-Supplemented Maturation Medium Enhances Cytoplasmic And Nuclear Maturation In Bovine Oocytes, Renata Blocher, Ying Liu, Tayler Patrick, Irina A. Polejaeva

Animal, Dairy, and Veterinary Science Faculty Publications

Bovine in vitro oocyte maturation (IVM) is an easy way to obtain oocytes for subsequent assisted reproductive techniques but is inefficient compared to in vivo maturation. Supplementation of three cytokines, fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1), or FLI, has increased oocyte maturation and embryo development in multiple species, but studies have not explored the oocyte differences caused by FLI IVM supplementation. This study aimed to assess important nuclear and cytoplasmic maturation events in high-quality oocytes. FLI-supplemented oocytes had a decreased GV (3.0% vs. 13.7%, p < 0.01) and increased telophase I incidence (34.6% vs. 17.6%, p < 0.05) after IVM, increased normal meiotic spindles (68.8% vs. 50.0%, p < 0.001), and an increased nuclear maturation rate (75.1% vs. 66.8%, p < 0.001). Moreover, in metaphase II oocytes, the percentage of FLI-treated oocytes with a diffuse mitochondrial distribution was higher (87.7% vs. 77.5%, p < 0.05) and with a cortical mitochondrial distribution was lower (11.6% vs. 17.4%, p < 0.05). Additionally, FLI-supplemented oocytes had more pattern I cortical granules (21.3% vs. 14.4%, p < 0.05). These data suggest that FLI supplementation in bovine in vitro maturation medium coordinates nuclear and cytoplasmic maturation to produce higher-quality oocytes.

Influence Of Steroidal Implants And Zinc Sulfate Supplementation On Growth Performance, Trace Mineral Status, Circulating Metabolites, And Transcriptional Changes In Skeletal Muscle Of Feedlot Steers, Dathan T. Smerchek, Emma L. Rients, Amy M. Mclaughlin, Kara J. Thornton, Stephanie L. Hansen

Animal, Dairy, and Veterinary Science Faculty Publications

Angus-cross steers (n = 144; 362 kg ± 20.4) were used to determine the effect of Zn and steroidal implants on performance, trace mineral status, circulating metabolites, and transcriptional changes occurring in skeletal muscle. Steers (n = 6 per pen) were stratified by body weight (BW) in a 3 x 2 factorial. GrowSafe bunks recorded individual feed intake (steer as experimental unit; n = 24 per treatment). Dietary treatments (ZINC; eight pens per treatment) included supplemental Zn as ZnSO₄ at 1) 0 (analyzed 54 mg Zn/kg DM; Zn0); 2) 30 mg/kg DM ( …

Cocoa Polyphenols Modulate The Fecal Microbiome In A Mouse Model Of Colitis, Independent Of Basal Diet And Colon Inflammation, Abby D. Benninghoff

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Chronic inflammation increases the risk of developing multiple chronic diseases, including cancer. The risk of developing colorectal cancer (CRC) specifically is increased in individuals who suffer from colitis, a characteristic of Inflammatory Bowel Disease (IBD). Diet is another risk factor for developing CRC, particularly an inflammation-promoting Western-type diet. Thus, supplementing a Western diet with functional foods containing anti-inflammatory polyphenols is a potential approach to decreasing CRC risk by reducing gut inflammation and altering gut microbiome profile. This study aimed to determine the effects of cocoa polyphenol (CP) supplementation on inflammation status and microbiome profile before, during, and after colitis when …

Colloidal Calcium Phosphate (Ccp) Causes Problematic Acid Variation In Cheddar Cheese, Sakshi Singh

Research on Capitol Hill

pH variation in cheese during the early ripening stages causes changes in the texture, body, and flavor of cheese. The dairy industry, encompassing over 50,000 dairy farms nationwide and several regional dairy farmers and companies in Utah, suffers substantial financial loss as cheese with varying pH is rendered mostly useless.

Efficacy Of A Laser As A Starling Deterrent And Its Effect On Lactating Dairy Cow Behavior, Callan A. Lichtenwalter, Marcos Marcondes, Kyle R. Taylor, Craig Mcconnel, Amber Adams Progar

Human–Wildlife Interactions

European starlings (Sturnus vulgaris) cause damage (including structural damage) on dairies, eat cattle feed, and can potentially spread disease through their fecal matter. Deterring starlings from dairies without affecting cow (Bos taurus) welfare is vital, and lasers have been effective starling deterrence tools in urban roosts and in some crops. To evaluate if the use of lasers on dairies would affect starling use of freestall barns as night roosts, or lactating cow behavior, 1 laser was installed in each of the 2 freestall barns at the Knott Dairy Center at Washington State University, Pullman, Washington, USA. …

Characterization Of Enterovirus D68 In A Mouse Model And Evaluation Of Antiviral Therapies, Zoe K. Taylor

All Graduate Theses and Dissertations, Fall 2023 to Present

Enterovirus D68 (EV-D68) is a virus that causes respiratory disease. Children are most commonly affected by EV-D68 but immunocompromised adults can also get infected and develop severe disease signs. In 2014, the United States experienced an unprecedented outbreak of EV-D68. Similar outbreaks were also observed in 2016, 2018 and 2022. Interestingly, few EV-D68 infections were observed in 2020, likely due to public health measures put in place to reduce the spread of another respiratory disease, coronavirus disease 2019 (COVID-19). EV-D68 is in the same virus family as polio, and one of the most concerning findings from these outbreaks is that …

Assessing The Needs Of Utah Dairy Farmers To Inform Extension Programming, Bruce F. Richards, Lendel Narine, Justin Clawson, Kalen Taylor, Jacob Hadfield, Jody Gale

Outcomes and Impact Quarterly

A needs assessment was conducted by the Utah State University Dairy Extension Team to identify the most urgent issues faced by Utah dairy farmers. The assessment found that the top five priority needs were implementing new technology, improving public perception of water use, managing risk, succession planning, and reducing feed costs. The results will guide dairy Extension programming in Utah. Several workshops and events have already been planned to address these needs. Overall, the assessment will help USU Extension provide research-based educational programs to meet the needs of the dairy industry in Utah.