Dairy Science Commons^™

Cross-Reactivity Of 15 Jev Antigen-Directed Rabbit Antisera With Three Clinically Important Jev Serogroup Members: Wnv, Mvev, And Slev, Sang-Im Yun, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Japanese encephalitis virus (JEV), the prototype member of the JEV serogroup within the genus Orthoflavivirus (family Flaviviridae), is closely related to West Nile virus (WNV), Murray Valley encephalitis virus (MVEV), and St. Louis encephalitis virus (SLEV). To characterize antigenic cross-reactivity within this group, we evaluated 15 region-specific rabbit antisera, previously generated against nearly the entire JEV protein-coding region, by immunoblotting whole-cell lysates from BHK-21 cells infected with WNV, MVEV, or SLEV, with JEV included as a reference. Six antisera (α-E^N-term, α-NS2B, α-NS3^N-term, α-NS3^C-term, α-NS5^N-term, and α-NS5^C-term) robustly recognized homologous …

Coenzyme Q10 Improves Functional And Structural Parameters Of Dairy Goat Sperm During Cooling And Cryopreservation, Ranadheer Narlagiri, Abdallah M. Shahat, Courtney Henry, Ashvini Pawar, Niki C. Whitley, Iman B. Shaheed, Mahipal Singh, Brou Kouakou, Irina A. Polejaeva, Adel R. Moawad

Animal, Dairy, and Veterinary Science Faculty Publications

Cryopreservation of gametes is crucial for conserving genetic diversity in livestock and endangered species, but the process can significantly impair sperm quality due to oxidative stress. Our aim was to evaluate the impacts of coenzyme Q10 (CoQ10) supplementation on the in vitro quality of cooled and cryopreserved goat semen. Semen samples collected from six mature Saanen bucks were pooled then diluted with AndroMed^® semen extender to a final concentration of 800 × 10⁶ sperm/mL. Diluted semen was supplemented with 0, 1, 2, 5, 10, and 20 µM CoQ10. Extended semen was either cooled at 4 °C for 72 …

A Bovine Cell Line Resistant To Japanese Encephalitis Virus Entry But Permissive To Post-Entry Replication, Sang-Im Yun, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Japanese encephalitis virus (JEV) is a mosquito-borne zoonotic orthoflavivirus that poses a significant global health threat. It causes severe neuroinflammatory disease in humans and reproductive failure in swine. Because of the broad host range and cell tropism of JEV, identifying animal cell lines resistant to infection has been a persistent challenge. In this study, we demonstrate that Madin–Darby bovine kidney (MDBK) cells are resistant to JEV infection yet remain fully permissive to viral replication when transfected with viral genomic RNA. Using immunoblotting, immunofluorescence, and flow cytometry, we show that MDBK cells, unlike the highly susceptible baby hamster kidney (BHK-21) cells …

Effect Of The Ahr Inhibitor Ch223191 As An Adjunct Treatment For Mammarenavirus Infections, Miguel Angel Pelaez, Jonna B. Westover, Dionna Scharton, Cybele Carina García, Brian B. Gowen

Animal, Dairy, and Veterinary Science Faculty Publications

The family Arenaviridae encompasses zoonotic, rodent-borne pathogens (e.g., Lassa, Machupo, and Junín viruses) that cause severe viral hemorrhagic fevers with high case fatality rates. The current therapeutic landscape is severely limited, underscoring the urgent need for novel antiviral strategies. A promising approach involves combining directly acting antivirals with host-targeted antivirals. A compelling host-targeted antiviral target is the aryl hydrocarbon receptor (AHR). This ubiquitous ligand-activated transcription factor is a recognized pro-viral host factor across multiple viral families. Building on prior work with Junín and Tacaribe viruses, we investigated whether the AHR inhibitor CH223191 could enhance the virus-directed antiviral activity of favipiravir …

Homologous And Heterologous Vaccination Regimens With Mrna And Rvsv Platforms Induce Potent Immune Responses Against Sftsv Glycoprotein, Tomaz B. Manzoni, Jonna B. Westover, Kendall A. Lundgreen, Philip D. Hicks, Raegan J. Petch, Jordan T. Ort, Drew Weissman, Steven H. Y. Fan, Scott E. Hensley, Norbert Pardi, Brian B. Gowen, Paul Bates

Animal, Dairy, and Veterinary Science Faculty Publications

Background: Severe fever with thrombocytopenia syndrome virus (SFTSV) is a highly pathogenic bunyavirus with a high case-fatality ratio for which there is no approved vaccine. Studies have assessed different vaccine technologies. However, few studies have yet assessed the immunogenicity of heterologous prime-boost regimens. Methods: Here, we compare a lipid nanoparticle (LNP)-encapsulated nucleoside-modified mRNA-based vaccine encoding the SFTSV glycoproteins, Gn and Gc, to our recently described recombinant VSV SFTSV (rVSV-SFTSV) vaccine in single dose, homologous, and heterologous prime-boost regimens in mice. Results: We show that all regimens protect from pathogenic SFTSV challenge and elicit strong long-lasting antibody responses. Furthermore, strong cellular …

Evaluating A Proprietary Tannin-Blend Product As An Alternative To Monensin And Tylosin Phosphate In Feedlot Cattle Diets, Luana D. Felizari, Luke K. Fuerniss, Jonathon L. Beckett, David S. Secrist, Guy D. Hufstedler, Bradley J. Johnson

Animal, Dairy, and Veterinary Science Faculty Publications

This study evaluates the effects of a proprietary tannin blend (BX), supplemented with or without sodium monensin (MON), on beef cattle performance, carcass traits, and health. Steers (n = 2986; initial shrunk body weight (SBW) 254 ± 9.2 kg) were allocated into 48 pens (61–62 steers/pen; 12 pens/treatment) fed for 230 d. Treatments included (1) no feed additives (CTL); (2) BX (7.95 g/animal daily); (3) MON + Tylosin phosphate (TYL; 437.52 mg MON/animal daily + 80 mg TYL/animal daily); or (4) MON+BX (437.52 mg MON/animal daily + 7.95 g BX/animal daily). Data were analyzed in R 4.2.1 using a …

Genomic-Transcriptomic Analysis Identifies The Syrian Hamster As A Superior Animal Model For Human Diseases, Chuchu Wang, Zhenguo Cheng, Jinxin Miao, Xia Xue, Yunshu Dong, Li Zhao, Haoran Guo, Jianyao Wang, Zhizhong Wang, Shuangshuang Lu, Guangming Fang, Ying Peng, Yafei Zhai, Zhongxian Zhang, Dongling Gao, Zhimin Wang, Pengju Wang, Lirong Zhang, Louisa S. Chard Dunmall, Jun Wang, Wenxue Tang, Xiaowei Li, Zhongren Ding, Xiaoyan Zhao, Ling Li, Nicholas R. Lemoine, Zhongde Wang, Daniel Tonge, Wenjie Tan, Jianzeng Dong, Yaohe Wang

Animal, Dairy, and Veterinary Science Faculty Publications

Background The Syrian hamster (Mesocricetus auratus) has shown promise as a human diseases model, recapitulating features of different human diseases including COVID-19. However, the landscape of its genome and transcriptome has not been systematically dissected, restricting its potential applications.

Results Here we provide a complete analysis of the genome and transcriptome of the Syrian hamster and found that its lineage diverged from that of the Chinese hamster (Cricetulus griseus) around 29.4 million years ago. 21,387 protein-coding genes were identified, with 90.03% of the 2.56G base pair sequence being anchored to 22 chromosomes. Further comparison of the …

Species Differences In The Biotransformation Of Aflatoxin B1: Primary Determinants Of Relative Carcinogenic Potency In Different Animal Species, David L. Eaton, David E. Williams, Roger A. Coulombe

Animal, Dairy, and Veterinary Science Faculty Publications

It has been known since the early days of the discovery of aflatoxin B1 (AFB1) that there were large species differences in susceptibility to AFB1. It was also evident early on that AFB1 itself was not toxic but required bioactivation to a reactive form. Over the past 60 years there have been thousands of studies to delineate the role of ~10 specific biotransformation pathways of AFB1, both phase I (oxidation, reduction) and phase II (hydrolysis, conjugation, secondary oxidations, and reductions of phase I metabolites). This review provides a historical context and substantive analysis of each of these pathways as contributors …

Safety, Immunogenicity, And Efficacy Of A Recombinant Vesicular Stomatitis Virus Vectored Vaccine Against Severe Fever With Thrombocytopenia Syndrome Virus And Heartland Bandavirus, Philip Hicks, Tomaz B. Manzoni, Jonna B. Westover, Raegan J. Petch, Brianne Roper, Brian B. Gowen, Paul Bates

Animal, Dairy, and Veterinary Science Faculty Publications

Background: Severe fever with thrombocytopenia syndrome virus (STSV) is a recently emerged tickborne virus in east Asia with over 18,000 confirmed cases. With a high case fatality ratio, SFTSV has been designated a high priority pathogen by the WHO and the NIAID. Despite this, there are currently no approved therapies or vaccines to treat or prevent SFTS. Vesicular stomatitis virus (VSV) represents an FDA-approved vaccine platform that has been considered for numerous viruses due to its low sero-prevalence in humans, ease in genetic manipulation, and promiscuity in incorporating foreign glycoproteins into its virions. Methods: In this study, we developed a …

Differential Hepatic Expression Of Mirna In Response To Aflatoxin B1 Challenge In Domestic And Wild Turkeys, Kade Jorud, Kristelle M. Mendoza, Thomas Kono, Roger A. Coulombe, Kent M. Reed

Animal, Dairy, and Veterinary Science Faculty Publications

Aflatoxin B1 (AFB₁) is a major foodborne mycotoxin that poses a significant economic risk to poultry due to a greater degree of susceptibility compared to other agricultural species. Domesticated turkeys (Meleagris gallopavo) are especially sensitive to AFB₁; however, wild turkeys (M. g. silvestris) are more resistant. A lack of functional isoforms of hepatic glutathione S-transferases (GSTs), an enzyme that plays a role in the detoxification of aflatoxin, is suspected as the reason for the increased sensitivity. Previous studies comparing the gene expression of domesticated and wild turkeys exposed to AFB₁ identified …

Effects Of Premortem Stress On Protein Expression, Steak Color, Oxidation, And Myofibrillar Fragmentation Index In The Longissimus Lumborum, Reganne K. Briggs, Jerrad F. Legako, Paul R. Broadway, Jeff A. Carroll, Nicole C. Burdick Sanchez, Nikole E. Ineck, Zachary K. Smith, Ranjith Ramanathan, Kara J. Thornton

Animal, Dairy, and Veterinary Science Faculty Publications

No abstract provided.

Expression And Secretion Of Sparc, Fgf-21 And Dcn In Bovine Muscle Cells: Effects Of Age And Differentiation, Katie A. Shira, Kara J. Thornton, Brenda M. Murdoch, Gabrielle M. Becker, Gwinyai E. Chibisa, Gordon K. Murdoch

Animal, Dairy, and Veterinary Science Faculty Publications

Skeletal muscle growth is an economically important trait in the cattle industry. Secreted muscle-derived proteins, referred to as myokines, have important roles in regulating the growth, metabolism, and health of skeletal muscle in human and biomedical research models. Accumulating evidence supports the importance of myokines in skeletal muscle and whole-body health, though little is known about the potential presence and functional significance of these proteins in cattle. This study evaluates and confirms that secreted proteins acidic and rich in cysteine (SPARC), fibroblast growth factor 21 (FGF-21), myostatin (MSTN), and decorin (DCN) are …

Cytokine-Supplemented Maturation Medium Enhances Cytoplasmic And Nuclear Maturation In Bovine Oocytes, Renata Blocher, Ying Liu, Tayler Patrick, Irina A. Polejaeva

Animal, Dairy, and Veterinary Science Faculty Publications

Bovine in vitro oocyte maturation (IVM) is an easy way to obtain oocytes for subsequent assisted reproductive techniques but is inefficient compared to in vivo maturation. Supplementation of three cytokines, fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1), or FLI, has increased oocyte maturation and embryo development in multiple species, but studies have not explored the oocyte differences caused by FLI IVM supplementation. This study aimed to assess important nuclear and cytoplasmic maturation events in high-quality oocytes. FLI-supplemented oocytes had a decreased GV (3.0% vs. 13.7%, p < 0.01) and increased telophase I incidence (34.6% vs. 17.6%, p < 0.05) after IVM, increased normal meiotic spindles (68.8% vs. 50.0%, p < 0.001), and an increased nuclear maturation rate (75.1% vs. 66.8%, p < 0.001). Moreover, in metaphase II oocytes, the percentage of FLI-treated oocytes with a diffuse mitochondrial distribution was higher (87.7% vs. 77.5%, p < 0.05) and with a cortical mitochondrial distribution was lower (11.6% vs. 17.4%, p < 0.05). Additionally, FLI-supplemented oocytes had more pattern I cortical granules (21.3% vs. 14.4%, p < 0.05). These data suggest that FLI supplementation in bovine in vitro maturation medium coordinates nuclear and cytoplasmic maturation to produce higher-quality oocytes.

Influence Of Steroidal Implants And Zinc Sulfate Supplementation On Growth Performance, Trace Mineral Status, Circulating Metabolites, And Transcriptional Changes In Skeletal Muscle Of Feedlot Steers, Dathan T. Smerchek, Emma L. Rients, Amy M. Mclaughlin, Kara J. Thornton, Stephanie L. Hansen

Animal, Dairy, and Veterinary Science Faculty Publications

Angus-cross steers (n = 144; 362 kg ± 20.4) were used to determine the effect of Zn and steroidal implants on performance, trace mineral status, circulating metabolites, and transcriptional changes occurring in skeletal muscle. Steers (n = 6 per pen) were stratified by body weight (BW) in a 3 x 2 factorial. GrowSafe bunks recorded individual feed intake (steer as experimental unit; n = 24 per treatment). Dietary treatments (ZINC; eight pens per treatment) included supplemental Zn as ZnSO₄ at 1) 0 (analyzed 54 mg Zn/kg DM; Zn0); 2) 30 mg/kg DM ( …

Mice As An Animal Model For Japanese Encephalitis Virus Research: Mouse Susceptibility, Infection Route, And Viral Pathogenesis, Jordan C. Frank, Byung-Hak Song, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Japanese encephalitis virus (JEV), a zoonotic flavivirus, is principally transmitted by hematophagous mosquitoes, continually between susceptible animals and incidentally from those animals to humans. For almost a century since its discovery, JEV was geographically confined to the Asia-Pacific region with recurrent sizable outbreaks involving wildlife, livestock, and people. However, over the past decade, it has been detected for the first time in Europe (Italy) and Africa (Angola) but has yet to cause any recognizable outbreaks in humans. JEV infection leads to a broad spectrum of clinical outcomes, ranging from asymptomatic conditions to self-limiting febrile illnesses to life-threatening neurological complications, particularly …

Understanding The Effects Of Trenbolone Acetate, Polyamine Precursors, And Polyamines On Proliferation, Protein Synthesis Rates, And The Abundance Of Genes Involved In Myoblast Growth, Polyamine Biosynthesis, And Protein Synthesis In Murine Myoblasts, Laura A. Motsinger, Lillian L. Okamoto, Nikole E. Ineck, Brynne A. Udy, Christopher L. Erickson, Youssef Harraq, Caleb C. Reichhardt, Gordon K. Murdoch, Kara Jean Thornton-Kurth

Animal, Dairy, and Veterinary Science Faculty Publications

Research suggests that androgens increase skeletal muscle growth by modulating polyamine biosynthesis. As such, the objective of this study was to investigate effects of anabolic hormones, polyamine precursors, and polyamines relative to proliferation, protein synthesis, and the abundance of mRNA involved in polyamine biosynthesis, proliferation, and protein synthesis in C2C12 and Sol8 cells. Cultures were treated with anabolic hormones (trenbolone acetate and/or estradiol), polyamine precursors (methionine or ornithine), or polyamines (putrescine, spermidine, or spermine). Messenger RNA was isolated 0.5 or 1, 12, or 24 h post-treatment. The cell type had no effect (p > 0.10) on proliferation, protein synthesis, or …

Basal Diet Fed To Recipient Mice Was The Driving Factor For Colitis And Colon Tumorigenesis, Despite Fecal Microbiota Transfer From Mice With Severe Or Mild Disease, Daphne Michelle Rodriguez Jimenez, Korry J. Hintze, Giovanni Rompato, Eliza C. Stewart, Abbey H. Barton, Emily Mortensen-Curtis, Porter A. Green, Arnaud J. Van Wettere, Aaron J. Thomas, Abby D. Benninghoff

Animal, Dairy, and Veterinary Science Faculty Publications

Consumption of the total Western diet (TWD) in mice has been shown to increase gut inflammation, promote colon tumorigenesis, and alter fecal microbiome composition when compared to mice fed a healthy diet, i.e., AIN93G (AIN). However, it is unclear whether the gut microbiome contributes directly to colitis-associated CRC in this model. The objective of this study was to determine whether dynamic fecal microbiota transfer (FMT) from donor mice fed either the AIN basal diet or the TWD would alter colitis symptoms or colitis-associated CRC in recipient mice, which were fed either the AIN diet or the TWD, using a 2 …

Lymphocyte Soluble Factors From Pregnant Cows Modulate Mrna Transcript Abundances Encoding For Proteins Associated With Trophoblast Growth And Development, Kelsy A. Leppo, Preston Collins, Kira Morgado, Ana C. Silva, Aaron Thomas, Heloisa M. Rutigliano

Animal, Dairy, and Veterinary Science Faculty Publications

This study was conducted to determine whether T cell populations are responsible for modulating placental development during gestation in cattle. It was hypothesized that CD4+CD25+ and γ/δ+ T cells modulate gene expression, based on mRNA transcript abundances, and promote proliferation and survival of trophoblast cells. Peripheral blood was collected from cows at 160 to 180 days of gestation and non-pregnant cows, T cell populations CD8+, CD4+, CD4+CD25+, CD24+CD25-, and γ/δ+ T cells were isolated, cultured for 48 h, and supernatant was collected. Placental samples were digested, and trophoblast cells were cultured for 24 h. Trophoblast cells were cultured with 50 …

Memantine Treatment Reduces The Incidence Of Flaccid Paralysis In A Zika Virus Mouse Model Of Temporary Paralysis With Similarities To Guillain-Barré Syndrome, Venkatraman Siddharthan, Hong Wang, Alexandre Lr De Oliveria, Xin Dai, John D. Morrey

Animal, Dairy, and Veterinary Science Faculty Publications

Clinical evidence suggests that Zika virus contributes to Guillain-Barré syndrome that causes temporary paralysis. We utilized a recently described Zika virus mouse model of temporary flaccid paralysis to address the hypothesis that treatment with an N-methyl-D-aspartate receptor antagonist, memantine, can reduce the incidence of paralysis. Aged interferon alpha/beta-receptor knockout mice were used because of their sublethal susceptibility to Zika virus infection. Fifteen to twenty-five percent of mice infected with a Puerto Rico strain of Zika virus develop acute flaccid paralysis beginning at days 8–9 and peaked at days 10–12. Mice recover from paralysis within a week of onset. In …

Association Between Single Nucleotide Polymorphisms In Sirt1 And Sirt2 Loci And Growth In Tibetan Sheep, Lin-Sheng Gui, Sayed Haidar Abbas Raza, Li Zhou, Matthew Garcia, Ayman Hassan Abd El-Aziz, Dawei Wei, Shengzhen Hou, Jianlei Jia, Zhiyou Wang

Animal, Dairy, and Veterinary Science Faculty Publications

Silent information regulator 1 and 2 (SIRT1, 2) were NAD+-dependent histone or non-histone deacetylase, which emerged as key metabolic sensors in several tissues of mammals. In the present study, the search for polymorphisms within the ovine SIRT1 and SIRT2 loci as well as association analyses between SNPs and growth-related traits were performed in Tibetan sheep. To determine the expression pattern of SIRT1 and SIRT2 genes in Tibetan sheep, the quantitative real-time polymerase chain reaction (qPCR) analysis revealed that those two genes were widely expressed in diverse tissues. Expression of SIRT1 was less in abomasum of lamb, whereas it …

Characterization Of A Novel Stat 2 Knock Out Hamster Model Of Crimean Congo Hemorrhagic Fever Virus Pathogenesis, Charlene Ranadheera, Emelissa J. Valcourt, Bryce M. Warner, Guillaume Poliquin, Kyle Rosenke, Kathy Frost, Kevin Tierney, Greg Saturday, Jinxin Miao, Jonna B. Westover, Brian B. Gowen, Stephanie Booth, Heinz Feldmann, Zhongde Wang, David Safronetz

Animal, Dairy, and Veterinary Science Faculty Publications

Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne pathogen causing a febrile illness in humans, which can progress to hemorrhagic manifestations, multi-organ failure, and death. Current mouse models of CCHFV infection reliably succumb to virus challenge but vary in their ability to reflect signs of disease similar to humans. In this study, we established a signal transducer and activator of transcription 2 (STAT2) knockout hamster model to expand the repertoire of animal models of CCHFV pathogenesis that can be used for therapeutic development. These hamsters demonstrated a systemic and lethal disease in response to infection. Hallmarks of human …

Raman Spectroscopy Characterization Extracellular Vesicles From Bovine Placenta And Peripheral Blood Mononuclear Cells, Han Zhang, Ana Caroline Silva, Wei Zhang, Heloisa M. Rutigliano, Anhong Zhou

Animal, Dairy, and Veterinary Science Faculty Publications

Placenta-derived extracellular vesicles (EVs) are involved in communication between the placenta and maternal immune cells possibly leading to a modulation of maternal T-cell signaling components. The ability to identify EVs in maternal blood may lead to the development of diagnostic and treatment tools for pregnancy complications. The objective of this work was to differentiate EVs from bovine placenta (trophoblast) and peripheral blood mononuclear cells (PBMC) by a label-free, non-invasive Raman spectroscopy technique. Extracellular vesicles were isolated by ultracentrifugation. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) were applied to verify the presence and the size distribution of EVs. Raman …

Comparison Of In Vitro Inactivation Of Sars Cov-2 With Hydrogen Peroxide And Povidone-Iodine Oral Antiseptic Rinses, Avinash S. Bidra, Jesse S. Pelletier, Jonna B. Westover, Samantha Frank, Seth M. Brown, Belachew Tessema

Animal, Dairy, and Veterinary Science Faculty Publications

Purpose

To evaluate the in vitro inactivation of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) with hydrogen peroxide (H2O2) and povidone‐iodine (PVP‐I) oral antiseptic rinses at clinically recommended concentrations and contact times.

Materials and Methods

SARS‐CoV‐2, USA‐WA1/2020 strain virus stock was prepared prior to testing by growing in Vero 76 cells. The culture media for prepared virus stock was minimum essential medium (MEM) with 2% fetal bovine serum (FBS) and 50 µg/mL gentamicin. Test compounds consisting of PVP‐I oral rinse solutions and H2O2 aqueous solutions were mixed directly with the virus solution so that the final concentration was 50% of …

Rapid In-Vitro Inactivation Of Severe Acute Respiratory Syndrome Coronavirus 2 (Sars-Cov-2) Using Povidone-Iodine Oral Antiseptic Rinse, Avinash S. Bidra, Jesse S. Pelletier, Jonna B. Westover, Samantha Frank, Seth M. Brown, Belachew Tessema

Animal, Dairy, and Veterinary Science Faculty Publications

Purpose

To investigate the optimal contact time and concentration for viricidal activity of oral preparation of povidone‐iodine (PVP‐I) against SARS‐CoV‐2 (‘corona virus’) to mitigate the risk and transmission of the virus in the dental practice.

Materials and Methods

The severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) USA‐WA1/2020 strain, virus stock was tested against oral antiseptic solutions consisting of aqueous povidone‐iodine (PVP‐I) as the sole active ingredient. The PVP‐I was tested at diluted concentrations of 0.5%, 1%, and 1.5%. Test media without any virus was added to 2 tubes of the compounds to serve as toxicity and neutralization controls. Ethanol (70%) …

Development, Characterization, And Application Of Two Reporter-Expressing Recombinant Zika Viruses, Sang-Im Yun, Byung-Hak Song, Michael E. Woolley, Jordan C. Frank, Justin G. Julander, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV), a mosquito-borne transplacentally transmissible flavivirus, is an enveloped virus with an ~10.8 kb plus-strand RNA genome that can cause neurological disease. To facilitate the identification of potential antivirals, we developed two reporter-expressing ZIKVs, each capable of expressing an enhanced green fluorescent protein or an improved luminescent NanoLuc luciferase. First, a full-length functional ZIKV cDNA clone was engineered as a bacterial artificial chromosome, with each reporter gene under the cap-independent translational control of a cardiovirus-derived internal ribosome entry site inserted downstream of the single open reading frame of the viral genome. Two reporter-expressing ZIKVs were then generated by …

Influence Of Total Western Diet On Docosahexaenoic Acid Suppression Of Silica-Triggered Lupus Flaring In Nzbwf1 Mice, Kristen N. Gilley, Kathryn A. Wierenga, Preeti S. Chauhaun, James G. Wagner, Ryan P. Lewandowski, Elizbeth A. Ross, A. L. Lock, Jack R. Harkema, Abby D. Benninghoff, James J. Pestka

Animal, Dairy, and Veterinary Science Faculty Publications

Lupus is a debilitating multi-organ autoimmune disease clinically typified by periods of flare and remission. Exposing lupus-prone female NZBWF1 mice to crystalline silica (cSiO₂), a known human autoimmune trigger, mimics flaring by inducing interferon-related gene (IRG) expression, inflammation, ectopic lymphoid structure (ELS) development, and autoantibody production in the lung that collectively accelerate glomerulonephritis. cSiO₂-triggered flaring in this model can be prevented by supplementing mouse diet with the ω-3 polyunsaturated fatty acid (PUFA) docosahexaenoic acid (DHA). A limitation of previous studies was the use of purified diet that, although optimized for rodent health, does not reflect the …

Evolutionary Selection Against Short Nucleotide Sequences In Viruses And Their Related Hosts, Yoram Zarai, Zohar Zafrir, Bunpote Siridechadilok, Amporn Suphatrakul, Modi Roopin, Justin G. Julander, Tamir Tuller

Animal, Dairy, and Veterinary Science Faculty Publications

Viruses are under constant evolutionary pressure to effectively interact with the host intracellular factors, while evading its immune system. Understanding how viruses co-evolve with their hosts is a fundamental topic in molecular evolution and may also aid in developing novel viral based applications such as vaccines, oncologic therapies, and anti-bacterial treatments. Here, based on a novel statistical framework and a large-scale genomic analysis of 2,625 viruses from all classes infecting 439 host organisms from all kingdoms of life, we identify short nucleotide sequences that are under-represented in the coding regions of viruses and their hosts. These sequences cannot be explained …

Theoretical Risk Of Genetic Reassortment Should Not Impede Development Of Live, Attenuated Rift Valley Fever (Rvf) Vaccines Commentary On The Draft Who Rvf Target Product Profile, Thomas P. Monath, Jeroen Kortekaas, Douglas M. Watts, Rebecca C. Christofferson, Angelle Desiree Labeaud, Brian B. Gowen, Clarence J. Peters, Darci R. Smith, Robert Swanepoel, John C. Morrill, Thomas G. Ksiazek, Phillip R. Pittman, Brian H. Bird, George Bettinger

Animal, Dairy, and Veterinary Science Faculty Publications

In November 2019, The World Health Organization (WHO) issued a draft set of Target Product Profiles (TPPs) describing optimal and minimally acceptable targets for vaccines against Rift Valley fever (RVF), a Phlebovirus with a three segmented genome, in both humans and ruminants. The TPPs contained rigid requirements to protect against genomic reassortment of live, attenuated vaccines (LAVs) with wild-type RVF virus (RVFV), which place undue constraints on development and regulatory approval of LAVs. We review the current LAVs in use and in development, and conclude that there is no evidence that reassortment between LAVs and wild-type RVFV has occurred during …

Consumption Of The Total Western Diet Promotes Colitis And Inflammation-Associated Colorectal Cancer In Mice, Abby D. Benninghoff, Korry J. Hintze, Stephany P. Monsanto, Daphne Michelle Rodriguez Jimenez, Ashli H. Hunter, Sumira Phatak, James J. Pestka, Arnaud J. Van Wettere, Robert E. Ward

Animal, Dairy, and Veterinary Science Faculty Publications

Consumption of a Western type diet is a known risk factor for colorectal cancer. Our group previously developed the total Western diet (TWD) for rodents with energy and nutrient profiles that emulate a typical Western diet. In this study, we tested the hypothesis that consumption of the TWD would enhance colitis, delay recovery from gut injury and promote colon tumorigenesis. In multiple experiments using the azoxymethane + dextran sodium sulfate or Apc^Min/+ mouse models of colitis-associated colorectal carcinogenesis (CAC), we determined that mice fed TWD experienced more severe and more prolonged colitis compared to their counterparts fed the standard …

Guinea Pig Transferrin Receptor 1 Mediates Cellular Entry Of Junín Virus And Other Pathogenic New World Arenaviruses, Brady T. Hickerson, Jonna B. Westover, Zhongde Wang, Young-Min Lee, Brian B. Gowen

Animal, Dairy, and Veterinary Science Faculty Publications

Several clade B New World arenaviruses (NWAs) can cause severe and often fatal hemorrhagic fever, for which preventive and therapeutic measures are severely limited. These NWAs use human transferrin receptor 1 (hTfR1) as a host cell receptor for virus entry. The most prevalent of the pathogenic NWAs is Junín virus (JUNV), the etiological agent of Argentine hemorrhagic fever. Small animal models of JUNV infection are limited because most laboratory rodent species are refractory to disease. Only guinea pigs are known to develop disease following JUNV infection, but the underlying mechanisms are not well characterized. In the present study, we demonstrate …